Mature cystic teratomas (MCTs) are the most common type of ovarian germ-cell tumor, rarely showing bladder invasion. We present a case of a premenopausal 47-year-old female who suffered from increased frequency to urinate followed by lower abdominal pain for 3 years. To the best of our knowledge, we present the first case of mature cystic ovarian teratoma invading the bladder, associated with lower abdominal pain after urination. Open partial cystectomy and lesion side accessory resection were done, which seemed effective in managing MCT that invades bladder.
{"title":"Mature cystic ovarian teratoma invading the bladder: A rare case report","authors":"Hong-xia Guo, Keqiang Yin, Yusheng Wang, Xu-nan Tong, Haosen Yang, Mancheng Xia, Weibing Shuang","doi":"10.4103/TS.TS_10_18","DOIUrl":"https://doi.org/10.4103/TS.TS_10_18","url":null,"abstract":"Mature cystic teratomas (MCTs) are the most common type of ovarian germ-cell tumor, rarely showing bladder invasion. We present a case of a premenopausal 47-year-old female who suffered from increased frequency to urinate followed by lower abdominal pain for 3 years. To the best of our knowledge, we present the first case of mature cystic ovarian teratoma invading the bladder, associated with lower abdominal pain after urination. Open partial cystectomy and lesion side accessory resection were done, which seemed effective in managing MCT that invades bladder.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125296828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xipeng Wang, K. Fukui, Mitsuteru Yokoyama, M. Tsuchiya, A. Kaneuji
Currently, elbow arthroplasty is used to treat elbow joint fracture, tumor, arthritis, and many other elbow-related diseases. However, due to the characteristics of the elbow joint, the success rate of elbow arthroplasty is lower than knee arthroplasty. The most common complication of this surgery is prosthesis infection and loosening. The focus of this case is on prosthesis infection after the elbow arthroplasty surgery.
{"title":"Postoperative infection of elbow joint replacement","authors":"Xipeng Wang, K. Fukui, Mitsuteru Yokoyama, M. Tsuchiya, A. Kaneuji","doi":"10.4103/TS.TS_8_18","DOIUrl":"https://doi.org/10.4103/TS.TS_8_18","url":null,"abstract":"Currently, elbow arthroplasty is used to treat elbow joint fracture, tumor, arthritis, and many other elbow-related diseases. However, due to the characteristics of the elbow joint, the success rate of elbow arthroplasty is lower than knee arthroplasty. The most common complication of this surgery is prosthesis infection and loosening. The focus of this case is on prosthesis infection after the elbow arthroplasty surgery.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125378632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: Intestinal fistula is a common condition with numerous infective complications. Adenocarcinoma in the anal fistula is a rare tumor, and most of the known cases have been documented as case reports. Osteomyelitis of the pelvis is uncommon. Whereas, adenocarcinoma associated with osteomyelitis of the pelvis and anal fistula is a rare case in clinical practice. We report an unusual case of multiple pelvic osteomyelitides, combined with intestinal tumors as a late complication following intestinal fistula. Methods: A case report and review of the pertinent English language literature. Results: To the best of our knowledge, this is the fist report in the English literature to describe multiple pelvic osteomyelitides combined with intestinal tumors as a serious complication following intestinal fistula. The diagnosis was made with the aid of computed tomography imaging, sinus imaging, X-ray film, needle aspiration biopsy, tumor marker tests, surgery, and pathological section. The complication was treated successfully with surgery. Conclusion: Multiple pelvic osteomyelitides combined with adenocarcinoma and anal fistula was indeed a rare case in clinical practice. Heightened awareness and better understanding of this complication would necessitate the early diagnosis and treatment.
{"title":"Multiple pelvic osteomyelitis combined with adenocarcinoma: An extremely rare complication following intestinal fistula","authors":"Zhen ping Zhang, Minghua Ji, Shuzhang Guo","doi":"10.4103/TS.TS_7_18","DOIUrl":"https://doi.org/10.4103/TS.TS_7_18","url":null,"abstract":"Aim: Intestinal fistula is a common condition with numerous infective complications. Adenocarcinoma in the anal fistula is a rare tumor, and most of the known cases have been documented as case reports. Osteomyelitis of the pelvis is uncommon. Whereas, adenocarcinoma associated with osteomyelitis of the pelvis and anal fistula is a rare case in clinical practice. We report an unusual case of multiple pelvic osteomyelitides, combined with intestinal tumors as a late complication following intestinal fistula. Methods: A case report and review of the pertinent English language literature. Results: To the best of our knowledge, this is the fist report in the English literature to describe multiple pelvic osteomyelitides combined with intestinal tumors as a serious complication following intestinal fistula. The diagnosis was made with the aid of computed tomography imaging, sinus imaging, X-ray film, needle aspiration biopsy, tumor marker tests, surgery, and pathological section. The complication was treated successfully with surgery. Conclusion: Multiple pelvic osteomyelitides combined with adenocarcinoma and anal fistula was indeed a rare case in clinical practice. Heightened awareness and better understanding of this complication would necessitate the early diagnosis and treatment.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126311545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neurodegenerative disease (ND) contains a range of conditions which are primarily characterized by progressive neuronal dysfunction and loss. ND is particularly difficult to cure, due to the vulnerability of the nervous system. Currently, few genes and pathways are found to be related to ND. However, no solitary mechanism appears to be primary in neurodegeneration, and these pathogenic mechanisms likely act synergistically through complex interactions to promote neurodegeneration. Moreover, the existing treatment is mainly focusing on alleviating the symptoms of the disease. Furthermore, recent studies reveal considerable overlaps of symptomatology and genetic risks across the disease subtypes by detailed studies at cellular, molecular and genetic level. To better understand the etiology and pathogenesis of ND, study of the abnormalities at patient level is most ideal. However, direct access to the brain tissues from healthy individuals and patients is very limited. Therefore, an alternative experimental model is required to study the mechanism of ND. The most commonly used models are animal models, including Caenorhabditis elegans, Drosophila, zebrafish, and genetically modified mice. However, animal models also have their limitations including partial recapitulation of the disease features and difficulties in modifying disease genes. The recent development in three-dimensional (3D) brain organoids might provide a better experimental model to study ND, because 3D brain organoid system carries great potential to expand the range of both physiological and pathological features that can be found during the development of disease, enabling higher order investigation of mechanism and functionality.
{"title":"Modeling neurodegenerative diseases by human pluripotent stem cell-induced brain organoid","authors":"Feng Tian, J. Hao, Longyu Hu, Xu Luo, Rui Sun","doi":"10.4103/TS.TS_4_18","DOIUrl":"https://doi.org/10.4103/TS.TS_4_18","url":null,"abstract":"Neurodegenerative disease (ND) contains a range of conditions which are primarily characterized by progressive neuronal dysfunction and loss. ND is particularly difficult to cure, due to the vulnerability of the nervous system. Currently, few genes and pathways are found to be related to ND. However, no solitary mechanism appears to be primary in neurodegeneration, and these pathogenic mechanisms likely act synergistically through complex interactions to promote neurodegeneration. Moreover, the existing treatment is mainly focusing on alleviating the symptoms of the disease. Furthermore, recent studies reveal considerable overlaps of symptomatology and genetic risks across the disease subtypes by detailed studies at cellular, molecular and genetic level. To better understand the etiology and pathogenesis of ND, study of the abnormalities at patient level is most ideal. However, direct access to the brain tissues from healthy individuals and patients is very limited. Therefore, an alternative experimental model is required to study the mechanism of ND. The most commonly used models are animal models, including Caenorhabditis elegans, Drosophila, zebrafish, and genetically modified mice. However, animal models also have their limitations including partial recapitulation of the disease features and difficulties in modifying disease genes. The recent development in three-dimensional (3D) brain organoids might provide a better experimental model to study ND, because 3D brain organoid system carries great potential to expand the range of both physiological and pathological features that can be found during the development of disease, enabling higher order investigation of mechanism and functionality.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124548089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Gul, E. Yuruk, M. Çulha, E. Şerefoğlu, A. Muslumanoglu
Aim: There are several treatment modalities for testosterone replacement therapy (TRT), including topical gels, subcutaneous testosterone pellets, transdermal patches, intramuscular injectables and oral forms. Despite the increasing usage of testosterone, there is limited information concerning patient adherence and compatibility of TRT. The aim of this study is to evaluate the factors which may have an impact on patients' adherence to TRT with topical gel. Methods: Between January 2013 and September 2013, 60 men from a tertiary urology clinic, who were prescribed 50 mg testosterone topical gel, were telephonically contacted to know if they continued or discontinued TRT, and when discontinued, the reasons for the same. Results: The mean age of the patients was 40.9 ± 9.9 (range: 21–59) years. The participation rate for the study through telephone was 51.6% (31/60). The most common reason for discontinued TRT was lack of perceived efficacy [n = 11 (35.5%)]. Factors, including age, weight, height, relationship status, and presence of comorbidity, were not associated with TRT adherence. The mean (standard error) time to TRT withdrawal was 5.9 (0.9) months. Conclusion: Most men voluntarily decided to discontinue testosterone and thus a close monitoring of patients by clinicians is essential to increase TRT adherence rate, with testosterone topical gel.
{"title":"Factors affecting adherence to testosterone replacement therapy","authors":"A. Gul, E. Yuruk, M. Çulha, E. Şerefoğlu, A. Muslumanoglu","doi":"10.4103/TS.TS_5_18","DOIUrl":"https://doi.org/10.4103/TS.TS_5_18","url":null,"abstract":"Aim: There are several treatment modalities for testosterone replacement therapy (TRT), including topical gels, subcutaneous testosterone pellets, transdermal patches, intramuscular injectables and oral forms. Despite the increasing usage of testosterone, there is limited information concerning patient adherence and compatibility of TRT. The aim of this study is to evaluate the factors which may have an impact on patients' adherence to TRT with topical gel. Methods: Between January 2013 and September 2013, 60 men from a tertiary urology clinic, who were prescribed 50 mg testosterone topical gel, were telephonically contacted to know if they continued or discontinued TRT, and when discontinued, the reasons for the same. Results: The mean age of the patients was 40.9 ± 9.9 (range: 21–59) years. The participation rate for the study through telephone was 51.6% (31/60). The most common reason for discontinued TRT was lack of perceived efficacy [n = 11 (35.5%)]. Factors, including age, weight, height, relationship status, and presence of comorbidity, were not associated with TRT adherence. The mean (standard error) time to TRT withdrawal was 5.9 (0.9) months. Conclusion: Most men voluntarily decided to discontinue testosterone and thus a close monitoring of patients by clinicians is essential to increase TRT adherence rate, with testosterone topical gel.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116723477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Nadeem, S. Mansoor, Shoab Saadat, Nadia Mehboob, H. Khan, Salman Assad, Bazeela Saeed, A. Saleem, Anam Zehra, A. Bukhari
Aim: Patients with chronic pain mostly suffer from additional psychiatric condition especially depression. The aim of this study is to determine a relation between depression and backache. Methods: We conducted a study between January 2015 and November 2015 at a tertiary care hospital in Islamabad, Pakistan. The study included 165 adult patients (≥20 years) with backache and were interviewed at the neurosurgery clinic. Visual analog scale (VAS) and Beck depression inventory (BDI) scores were analyzed. Results: According to VAS, 11.5% patients had mild, 67.2% patients had moderate, and 21.8% patients had severe backache. A striking difference in the frequency of depression was observed between the genders, 67.8% in females compared to 32.2% in males. BDI scores showed 12.1% normal, 13.9% mild, 3% borderline, 26% moderate, 24.8% severe, and 19.3% extreme depression. A statistically significant correlation was observed between severity of backache and depression (P < 0.003). Conclusion: A high number of patients suffering from backache also demonstrated mild to extreme depression. We recommend screening for depression among these patients to achieve a better treatment outcome.
{"title":"Visual analog scale and beck depression inventory assessing the clinical correlation of backache with depression","authors":"Muhammad Nadeem, S. Mansoor, Shoab Saadat, Nadia Mehboob, H. Khan, Salman Assad, Bazeela Saeed, A. Saleem, Anam Zehra, A. Bukhari","doi":"10.4103/TS.TS_6_18","DOIUrl":"https://doi.org/10.4103/TS.TS_6_18","url":null,"abstract":"Aim: Patients with chronic pain mostly suffer from additional psychiatric condition especially depression. The aim of this study is to determine a relation between depression and backache. Methods: We conducted a study between January 2015 and November 2015 at a tertiary care hospital in Islamabad, Pakistan. The study included 165 adult patients (≥20 years) with backache and were interviewed at the neurosurgery clinic. Visual analog scale (VAS) and Beck depression inventory (BDI) scores were analyzed. Results: According to VAS, 11.5% patients had mild, 67.2% patients had moderate, and 21.8% patients had severe backache. A striking difference in the frequency of depression was observed between the genders, 67.8% in females compared to 32.2% in males. BDI scores showed 12.1% normal, 13.9% mild, 3% borderline, 26% moderate, 24.8% severe, and 19.3% extreme depression. A statistically significant correlation was observed between severity of backache and depression (P < 0.003). Conclusion: A high number of patients suffering from backache also demonstrated mild to extreme depression. We recommend screening for depression among these patients to achieve a better treatment outcome.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130318728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kai Wang, Guang Yang, C. Han, Wei-Xuan Bi, Gui-xiang Zhang
Aim: The aim of this study was to discuss the common complications induced by long nasointestinal tubes (LTs) and prophylactic therapeutic measures. Methods: A retrospective analysis was performed, consisting of 88 patients treated with LT for acute bowel obstruction (including 61 patients who underwent plication of the small intestine by LT). Our study included 75 patients with an adhesive bowel obstruction, 5 patients with a malignant intestinal obstruction, 4 patients with an early postoperative inflammatory bowel obstruction, and 4 patients with a fecal obstruction. Results: The prevalence of nasopharyngeal discomfort and pain was 95.4%, and 44.3% of patients had an electrolyte disorder and another 3.4% were diagnosed with aspiration pneumonia. Among 25% of patients who had catheter-related accidents (15.9% with tube obstructions, 5.6% with unexpected catheter shedding, 1.1% with anterior balloon rupture, and 2.3% with catheter expelled from the anus), no tube breakage occurred. Conclusion: Nasopharyngeal discomfort and pain, electrolyte disorders, and tube obstructions are common complications when using LTs. Although their incidence rate is low, aspiration pneumonia, intestinal hemorrhage, perforation, and necrosis are more severe complications. Such severe complications should be taken seriously, and prophylactic therapeutic measures must be taken to minimize their occurrence. In general, the use of LTs is safe, effective, and worthy of wide use in clinical practice.
{"title":"Clinical analysis of common complications induced by long nasointestinal tubes: A retrospective cohort study","authors":"Kai Wang, Guang Yang, C. Han, Wei-Xuan Bi, Gui-xiang Zhang","doi":"10.4103/TS.TS_22_17","DOIUrl":"https://doi.org/10.4103/TS.TS_22_17","url":null,"abstract":"Aim: The aim of this study was to discuss the common complications induced by long nasointestinal tubes (LTs) and prophylactic therapeutic measures. Methods: A retrospective analysis was performed, consisting of 88 patients treated with LT for acute bowel obstruction (including 61 patients who underwent plication of the small intestine by LT). Our study included 75 patients with an adhesive bowel obstruction, 5 patients with a malignant intestinal obstruction, 4 patients with an early postoperative inflammatory bowel obstruction, and 4 patients with a fecal obstruction. Results: The prevalence of nasopharyngeal discomfort and pain was 95.4%, and 44.3% of patients had an electrolyte disorder and another 3.4% were diagnosed with aspiration pneumonia. Among 25% of patients who had catheter-related accidents (15.9% with tube obstructions, 5.6% with unexpected catheter shedding, 1.1% with anterior balloon rupture, and 2.3% with catheter expelled from the anus), no tube breakage occurred. Conclusion: Nasopharyngeal discomfort and pain, electrolyte disorders, and tube obstructions are common complications when using LTs. Although their incidence rate is low, aspiration pneumonia, intestinal hemorrhage, perforation, and necrosis are more severe complications. Such severe complications should be taken seriously, and prophylactic therapeutic measures must be taken to minimize their occurrence. In general, the use of LTs is safe, effective, and worthy of wide use in clinical practice.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115623215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: This study aimed to investigate the role of angiopoietin-2 (Ang-2) in the local control of malignant pleural effusion (MPE) and survival in patients with primary lung adenocarcinoma. Methods: Pleural effusion and blood samples were obtained from 85 patients with lung adenocarcinoma and MPE. Enzyme-linked immunosorbent assay was performed to investigate the expression levels of Ang-2 in pleural effusions and serums. SPSS software (Version 18.0) was used to analyze the data. Results: We found that the expression levels of Ang-2 in pleural effusion and serum were 26.82 ± 8.82 pg/mL and 358.78 ± 95.93 pg/mL, respectively. The expression level of Ang-2 in pleural effusion was found to be correlated with the control of pleural effusion. Based on receiver operating characteristic curve analysis, we found that when the cutoff value was 25.57 pg/mL, the efficacy of pleural effusion Ang-2 level in evaluating the control of pleural effusion could get the best result. Both the pleural effusion Ang-2 level and the local control of pleural effusion are independent factors affecting the prognosis of patients with lung cancer and MPE. However, this study also found that serum Ang-2 levels had no significant correlation with MPE local control or prognosis. Conclusion: Pleural effusion Ang-2 levels can be used as a molecular biomarker to predict both the MPE local control and prognosis of the MPE patients, which has significant clinical value.
{"title":"Role of Angiopoietin-2 in the control of malignant pleural effusion and survival in patients with primary lung adenocarcinoma","authors":"Xiaoxia Li, Wenkui Sun","doi":"10.4103/TS.TS_25_17","DOIUrl":"https://doi.org/10.4103/TS.TS_25_17","url":null,"abstract":"Aim: This study aimed to investigate the role of angiopoietin-2 (Ang-2) in the local control of malignant pleural effusion (MPE) and survival in patients with primary lung adenocarcinoma. Methods: Pleural effusion and blood samples were obtained from 85 patients with lung adenocarcinoma and MPE. Enzyme-linked immunosorbent assay was performed to investigate the expression levels of Ang-2 in pleural effusions and serums. SPSS software (Version 18.0) was used to analyze the data. Results: We found that the expression levels of Ang-2 in pleural effusion and serum were 26.82 ± 8.82 pg/mL and 358.78 ± 95.93 pg/mL, respectively. The expression level of Ang-2 in pleural effusion was found to be correlated with the control of pleural effusion. Based on receiver operating characteristic curve analysis, we found that when the cutoff value was 25.57 pg/mL, the efficacy of pleural effusion Ang-2 level in evaluating the control of pleural effusion could get the best result. Both the pleural effusion Ang-2 level and the local control of pleural effusion are independent factors affecting the prognosis of patients with lung cancer and MPE. However, this study also found that serum Ang-2 levels had no significant correlation with MPE local control or prognosis. Conclusion: Pleural effusion Ang-2 levels can be used as a molecular biomarker to predict both the MPE local control and prognosis of the MPE patients, which has significant clinical value.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127795876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiangyi Sun, Pin Zhang, Lei Zhang, Jianning Zhao, Li-wu Zhou
Aim: To investigate the role of miR-564 in promoting the proliferation and differentiation of synovial mesenchymal stem cells (SMSCs) to chondrocytes. Methods: Third-generation SMSCs were used, and the experiments involved untreated SMSCs (control; Group A), SMSCs transfected with Hsa-miR-564 inhibitor NC (inhibitor blank; Group B), and SMSCs transfected with Hsa-miR-564 inhibitor (Group C). The expression of miR-564 in SMSCs was determined by real-time quantitative polymerase chain reaction. The SMSCs were induced to form cartilage for 3 weeks. The morphology of the induced chondrocytes was observed by hematoxylin and eosin and toluidine blue staining and cell viability recorded. Chondrocyte differentiation of SMSCs related to genes and proteins (COL2A1, Aggrecan, SOX9, transforming growth factor beta 1 [TGF-β1], and Smad4) was assessed. The chondrogenic effect of miR-564 was examined after blocking the target gene TGF-β1. Results: The morphology and characteristics of the induced cells were consistent with those of chondrocytes. The cell proliferative rate of Group C (miR-564 downregulation) was significantly higher than that of other groups. The expression of genes and proteins related to chondrocyte differentiation was significantly decreased in Group C. The relative expression of genes related to cartilage differentiation decreased after blocking TGF-β1. Conclusion: The downregulation mediated by miR-564 can promote the differentiation and proliferation of SMSCs into chondrocytes by targeting TGF-β1.
{"title":"MicroRNA-564 promotes the differentiation and proliferation of synovial mesenchymal stem cells into chondrocytes by targeting transforming growth factor beta 1","authors":"Xiangyi Sun, Pin Zhang, Lei Zhang, Jianning Zhao, Li-wu Zhou","doi":"10.4103/TS.TS_23_17","DOIUrl":"https://doi.org/10.4103/TS.TS_23_17","url":null,"abstract":"Aim: To investigate the role of miR-564 in promoting the proliferation and differentiation of synovial mesenchymal stem cells (SMSCs) to chondrocytes. Methods: Third-generation SMSCs were used, and the experiments involved untreated SMSCs (control; Group A), SMSCs transfected with Hsa-miR-564 inhibitor NC (inhibitor blank; Group B), and SMSCs transfected with Hsa-miR-564 inhibitor (Group C). The expression of miR-564 in SMSCs was determined by real-time quantitative polymerase chain reaction. The SMSCs were induced to form cartilage for 3 weeks. The morphology of the induced chondrocytes was observed by hematoxylin and eosin and toluidine blue staining and cell viability recorded. Chondrocyte differentiation of SMSCs related to genes and proteins (COL2A1, Aggrecan, SOX9, transforming growth factor beta 1 [TGF-β1], and Smad4) was assessed. The chondrogenic effect of miR-564 was examined after blocking the target gene TGF-β1. Results: The morphology and characteristics of the induced cells were consistent with those of chondrocytes. The cell proliferative rate of Group C (miR-564 downregulation) was significantly higher than that of other groups. The expression of genes and proteins related to chondrocyte differentiation was significantly decreased in Group C. The relative expression of genes related to cartilage differentiation decreased after blocking TGF-β1. Conclusion: The downregulation mediated by miR-564 can promote the differentiation and proliferation of SMSCs into chondrocytes by targeting TGF-β1.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127744440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Esophageal bronchogenic cysts located in inferior esophagus are rare. Here, we report on the case of a 12-year-old female patient who presented at our institution with dyspepsia and abdominal distention for 2 years with no other symptoms. Physical examination of abdomen and chest was negative, and laboratory tests did not show any abnormal results. However, computed tomography imaging showed a mass of about 8 cm × 8 cm × 4 cm in the lower esophagus compressing the stomach. The preoperative diagnosis was determined to be an inferior esophageal duplication cyst. A transabdominal excision of the cyst was performed 5 days after hospital admission. Pathology revealed an esophageal bronchogenic cyst. The case report and literature shows that differences exist between esophageal duplication cysts and esophageal bronchogenic cysts.
{"title":"Differentiation of esophageal duplication cysts from esophageal bronchogenic cysts: A case report and literature review","authors":"Jun Yang, T. Zhou, Jiaqiang Fu, Yi-min Zhang","doi":"10.4103/TS.TS_1_18","DOIUrl":"https://doi.org/10.4103/TS.TS_1_18","url":null,"abstract":"Esophageal bronchogenic cysts located in inferior esophagus are rare. Here, we report on the case of a 12-year-old female patient who presented at our institution with dyspepsia and abdominal distention for 2 years with no other symptoms. Physical examination of abdomen and chest was negative, and laboratory tests did not show any abnormal results. However, computed tomography imaging showed a mass of about 8 cm × 8 cm × 4 cm in the lower esophagus compressing the stomach. The preoperative diagnosis was determined to be an inferior esophageal duplication cyst. A transabdominal excision of the cyst was performed 5 days after hospital admission. Pathology revealed an esophageal bronchogenic cyst. The case report and literature shows that differences exist between esophageal duplication cysts and esophageal bronchogenic cysts.","PeriodicalId":102077,"journal":{"name":"Translational Surgery","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125936494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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