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Effect of Potassium Source and Rate on Yield, Quality, and Tobacco-Specific Nitrosamines in Dark and Burley Tobacco 钾源和钾用量对黑烟和白肋烟产量、品质和烟草特异性亚硝胺的影响
Pub Date : 2021-01-01 DOI: 10.3381/tobsci-d-20-00001
Andrea B. Keeney, R. Pearce, W. Bailey
Field trials were conducted in Princeton, Murray, and Lexington, KY in 2016–2018 to determine response of dark and burley tobacco to potassium source (potassium sulfate or potassium chloride) and potassium rate (0, 93, 186, or 279 kg K ha−1). Field sites that showed higher potential for potassium yield response were selected based on low soil test potassium levels from soil samples collected in early spring each year. All potassium applications were made between 1 and 10 days before transplanting. Significant yield responses to potassium were seen in 5 of 12 trials at sites that had initial soil test potassium levels of ≤150 kg K ha−1. Although cured leaf chloride levels were >1% on average where potassium chloride was used, negative effects on cured leaf moisture were only seen in 1 of 12 trials, and negative effects on quality grade index were not seen in any trial. The most consistent effect of potassium chloride application seen in this research was a 28% reduction in average total tobacco-specific nitrosamines (TSNA) compared to potassium sulfate application. These results showing lower TSNA from potassium chloride applications, along with minimal effects on moisture and quality grade index, may cause the tobacco industry to reconsider the long-standing preference for potassium sulfate as the potassium source for tobacco production.
2016-2018年,在肯塔基州的普林斯顿、默里和列克星敦进行了田间试验,以确定黑烟和白烟对钾源(硫酸钾或氯化钾)和钾速率(0、93、186和279 kg K ha−1)的响应。根据每年早春采集的土壤样品中较低的土壤试验钾水平,选择钾产量响应潜力较大的大田立地。所有钾肥均在移栽前1 ~ 10天施用。在初始土壤试验钾含量≤150 kg K ha−1的地点进行的12个试验中,有5个试验对钾的产量有显著的响应。虽然施用氯化钾的烤烟叶片氯含量平均>1%,但12个试验中只有1个试验对烤烟叶片水分有负面影响,没有试验对品质等级指数有负面影响。在本研究中,氯化钾施用最一致的效果是与硫酸钾施用相比,烟草特异性亚硝胺(TSNA)平均总量减少28%。这些结果表明,氯化钾施用的TSNA较低,对湿度和质量等级指数的影响极小,可能会导致烟草行业重新考虑长期以来首选硫酸钾作为烟草生产钾源的做法。
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引用次数: 0
The Effect of Suckercide Application Timing and Cultivar Maturity on Chemical Topping of Burley Tobacco 杀乳剂施用时间和品种成熟度对白利烟化学打顶的影响
Pub Date : 2020-01-01 DOI: 10.3381/tobsci-d-20-00003
M. Richmond, R. Pearce, W. Bailey
Experiments were initiated in 2015 to evaluate the efficacy of chemical topping for burley tobacco (Nicotiana tabacum L.). The major objectives for this study were to determine the optimum timing of suckercide application and appropriate cultivar maturity for effective chemical topping. Burley tobacco cultivars TN 90 (medium maturity), KT 210, and KT 215 (late maturity) were chemically topped at the 10% button, 50% button, and 10% bloom growth stages. The 10% button and 50% button application timings were best suited for chemical topping practices. Treatments that targeted the 10% bloom stage did not completely halt inflorescence growth; however, all application timings resulted in excellent sucker control. Both medium and late maturing burley cultivars proved to be acceptable for chemical topping methods; however, timing the suckercide application may be less difficult with later maturing cultivars. Chemically topped treatments generally resulted in shorter, narrower tip leaves than manually topped treatments. There were no significant differences in total yield of TN 90 when comparing tobacco that was manually topped at 10% bloom to tobacco that was chemically topped at 10% button, 50% button, or 10% bloom across all environments. In 4 out of 6 environments, total yield was not significantly different between manual topping and any chemically topped application timing in the late maturing burley cultivars; however, at least 1 chemically topped application timing had equivalent yield to manually topped tobacco in all environments.
2015年启动了化学打头对白肋烟(Nicotiana tabacum L.)的药效评价试验。本研究的主要目的是确定最适宜的杀虫剂施用时间和适宜的品种成熟度,以便进行有效的化学打顶。白烟品种TN 90(中熟)、KT 210和KT 215(晚熟)分别在10%扣期、50%扣期和10%花期施用化学顶。10%按钮和50%按钮的施用时间最适合化学打顶。以10%开花期为目标的处理没有完全停止花序的生长;然而,所有的应用时机都产生了出色的吸盘控制。中、晚熟白肋品种均可采用化学打顶方法;然而,对于成熟较晚的品种来说,施用杀油剂的时机可能不那么困难。化学打顶处理通常比人工打顶处理产生更短、更窄的叶尖。在所有环境下,人工灌顶10%花期与化学灌顶10%花期、50%花期和10%花期烟草的TN 90总产量无显著差异。在6个环境中有4个环境下,晚熟白肋品种人工打顶与化学打顶的总产量差异不显著;然而,在所有环境中,至少1个化学顶施用时间与人工顶的产量相当。
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引用次数: 0
The Effect of Suckercide Product and Application Rate on Chemical Topping of Burley Tobacco 杀烟剂及其用量对白利烟化学打顶的影响
Pub Date : 2020-01-01 DOI: 10.3381/tobsci-d-22-00002
M. Richmond, R. Pearce, T. Mark, W. Bailey
The act of topping tobacco (Nicotiana tabacum L.) involves the removal of the terminal bud or inflorescence of the tobacco plant. This practice ordinarily is accomplished by manually removing the top of each tobacco plant in an entire field, which is labor intensive and costly. The major objectives for this research were to determine which labeled suckercides could be used effectively for chemical topping of burley tobacco and the effect of suckercide rate on sucker control, yield, leaf quality, maleic hydrazide (MH) residues, and leaf chemistry. A study was initiated at Murray, Princeton, and Lexington, KY that investigated the efficacy of suckercide applications using combinations of MH, butralin, and fatty alcohols (FA). The terminal bud was not well controlled with FA or butralin alone, nor was adequate sucker control or total yield achieved. A significant reduction in total yield and sucker control were observed when plants were chemically topped with MH alone compared to manually topped or chemically topped with a tank mixture of MH and butralin at Princeton only. At the other locations, all chemically topped plants had similar yield to manually topped plants. Our data suggested that chemical topping of burley tobacco with a tank mixture of MH and a local systemic can be an acceptable alternative to manual topping as total yield and leaf quality grade index were not significantly different at any location. Total tobacco-specific nitrosamine (TSNA) content and MH residues were significantly lower with chemical topping treatments in some years and locations.
打顶烟草(Nicotiana tabacum L.)的动作包括去除烟草植株的顶芽或花序。这种做法通常是通过人工去除整个田地里每棵烟草植株的顶部来完成的,这是劳动密集型的,成本很高。本研究的主要目的是确定哪些标记的杀烟剂可以有效地用于白烟的化学打顶,以及杀烟剂用量对白烟控制、产量、叶片品质、马来酰肼残留量和叶片化学性质的影响。普林斯顿的默里和肯塔基州的列克星敦发起了一项研究,调查了使用MH、丁曲林和脂肪醇(FA)组合的杀蝇剂的效果。单独使用FA或butralin不能很好地控制顶芽,也不能达到足够的吸盘控制或总产量。在普林斯顿,与人工灌顶或仅用氢化氢和丁曲林的罐内混合物进行化学灌顶相比,单独用氢化氢进行化学灌顶可显著降低植株的总产量和吸盘控制。在其他地点,所有化学顶的植物与人工顶的植物产量相似。我们的数据表明,在总产量和叶质量等级指数在任何地点都没有显著差异的情况下,用MH和局部系统混合的化学打顶可以替代人工打顶。在某些年份和地点,化学打顶处理显著降低了烟草特异性亚硝胺(TSNA)含量和MH残留。
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引用次数: 0
Effect of Ordering Method on Tobacco-Specific Nitrosamines (TSNAs) Content in Dark Air-Cured and Burley Tobacco 排序方式对暗烤烟和白利烟中烟草特异性亚硝胺含量的影响
Pub Date : 2020-01-01 DOI: 10.3381/tobsci-d-22-00003
M. Richmond, Colin Fisher, A. M. Fisher, R. Pearce, W. Bailey
Tobacco-specific nitrosamines (TSNAs) are known carcinogens in cured tobacco. They are produced primarily during the curing process, but agronomic practices occurring in the field as well as handling practices after curing may also influence TSNA levels, particularly if cured leaf is stored at high moisture. After curing and during market preparation, the cured leaf must be supple to avoid breakage. Ideally, this is after a period of wet weather during which the leaf absorbs moisture and comes into order or case. Often the weather remains dry for long periods after curing, and growers resort to artificial ordering to take down a sufficient amount of their crop to work on for several days, during which time the tobacco is bulked. The effect of this artificial ordering on TSNAs during short-term storage is not known. Field experiments were conducted in each of 3 years at two locations in Kentucky to evaluate TSNA accumulation following several ordering methods in dark air-cured and burley tobacco types. Treatments included natural ordering and variants of steaming and misting, which are both commonly used artificial ordering methods. At the Princeton location, samples were taken within 24 hr after the ordering treatments were done. In Lexington, samples were taken sequentially at takedown, after ordering, and after 14 d in the bulk. There were limited and inconsistent differences in total TSNAs between methods of ordering, and the TSNA levels were not affected by the moisture content of the leaf during bulking. There was a significant increase in TSNAs in the 24-hr period between takedown and bulking, which cannot be explained. We conclude that, in Kentucky, growers should use ordering methods that are best suited for their production system, but this may not be the case in warmer climates.
烟草特异性亚硝胺(TSNAs)是已知的在烤烟中的致癌物。它们主要是在烘烤过程中产生的,但田间的农艺做法以及烘烤后的处理做法也可能影响TSNA水平,特别是如果烘烤后的叶子在高湿度下储存。在固化后和市场准备期间,固化的叶子必须柔软,以避免破损。理想情况下,这是经过一段时间的潮湿天气期间,叶片吸收水分,并进入秩序或情况。通常情况下,干燥后的天气会持续很长一段时间,种植者就会采取人工命令的方式,取下足够数量的烟草来种植几天,在这段时间里,烟草会变大。这种人为排序在短期储存期间对tsna的影响尚不清楚。在肯塔基州的两个地点,每3年进行一次田间试验,以评估几种排序方法在暗风干和白肋烟类型中TSNA的积累。处理方法包括自然排序和蒸汽和喷雾的变体,这两种方法都是常用的人工排序方法。在普林斯顿地区,样品在排序处理完成后24小时内采集。在列克星敦,样品在下架时、下订单后和批量生产14天后依次采集。不同排序方法的总TSNA含量差异有限且不一致,且TSNA水平不受叶片膨胀过程中水分含量的影响。在取下和堆积之间的24小时内,TSNAs显著增加,这无法解释。我们的结论是,在肯塔基州,种植者应该使用最适合他们生产系统的订购方法,但在温暖的气候条件下,情况可能并非如此。
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引用次数: 0
Organic Sucker Control: Screening Different Active Ingredients for Commercial Application 有机吸盘控制:筛选不同的有效成分用于商业应用
Pub Date : 2020-01-01 DOI: 10.3381/tobsci-d-20-00008
M. M. Short, M. Vann, D. Suchoff
Organically derived fatty alcohol is useful for the control of tobacco axillary buds (suckers) and is greatly needed by commercial organic tobacco farmers. Recently, its approval by the U.S. Department of Agriculture (USDA)-National Organic Program has been scrutinized. The objective of this research was to evaluate the suggested alternatives: pelargonic acid, vegetable oil, canola oil, and peppermint + spearmint oil using two different application methods, a standard 3-nozzle boom or a dropline. Chemical injury was not observed within any treatment except for those containing pelargonic acid. Injury was greatest when applied with the 3-nozzle boom and was reduced by nearly 50% with the dropline; however, injury after the dropline application was 2.5 to 7 times greater than any other treatment. Despite significant injury, sucker control was acceptable with pelargonic acid (≈90%) and was similar to that resulting from fatty alcohol (99–100%). Sucker control was <40% among all other treatments, with peppermint + spearmint oil providing better efficacy than canola (10 to 15%) or vegetable oil (−1 to −10%). Cured leaf yield, quality, and value were likewise greatest in fatty alcohol treatments because of maximized sucker control and minimized chemical injury. Producers are encouraged to utilize fatty alcohol until the alternative products can be reformulated and re-evaluated.
有机衍生的脂肪醇对控制烟草腋芽(吸盘)很有用,商业有机烟农非常需要。最近,美国农业部(USDA)国家有机计划对其批准进行了审查。本研究的目的是评估建议的替代品:天竺葵酸、植物油、菜籽油和薄荷+绿薄荷油,使用两种不同的应用方法,标准的3喷嘴吊杆或滴管。除含有天竺葵酸的处理外,其他处理均未见化学损伤。使用3喷嘴动臂时损伤最大,使用下拉管可减少近50%的损伤;然而,应用吊索后的损伤是任何其他处理的2.5至7倍。尽管有明显的损伤,但使用天竺酸(≈90%)对吸盘的控制是可以接受的,与使用脂肪醇(99-100%)的效果相似。在所有其他处理中,吸盘控制率<40%,薄荷油+绿薄荷油的效果优于菜籽油(10 ~ 15%)或植物油(- 1 ~ - 10%)。同样,在脂肪醇处理下,叶片产量、品质和价值也最高,因为吸盘控制最大化,化学伤害最小化。鼓励生产商使用脂肪醇,直到替代产品可以重新配制和重新评估。
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引用次数: 0
Nitrogen Fertilizer Programs for Organic Flue-Cured Tobacco (Nicotiana Tabacum L.) Seedling Production 有机烤烟氮肥方案研究苗生产
Pub Date : 2020-01-01 DOI: 10.3381/tobsci-d-22-00001
D. Suchoff, M. Vann, M. McGinnis, J. H. Mason, L. Fisher
Certified organic flue-cured tobacco (Nicotiana tabacum L.) production has experienced significant expansion in the United States. Despite this expansion, there is very little information available that outlines organic nitrogen (N) programs for seedling production. To develop grower recommendations, research was conducted to evaluate the effects of a Peruvian seabird guano (SG), sodium nitrate (SN), or a combination of the two (SN_SG) in a float system on float water chemistry and seedling vigor. A conventional treatment (Conv; SQM Ultrasol Premium) was included for comparison. A greenhouse study was conducted twice between June 2016 and January 2017. Nitrogen fertilizer treatments were applied to tobacco float system water twice during the germination and growth of tobacco transplants. Float system water was collected every 5 days and analyzed for N forms, pH, dissolved oxygen, and bicarbonate. At the end of each experiment, transplant dimensions were measured and percent of usable plants collected. Float water bicarbonate concentration was <1 meq L−1 in treatments absent of SG for the duration of the study, but were in excess of 12 meq L−1 25 days after seeding (DAS) when SG was the exclusive N source. Despite high ammonium and bicarbonate concentrations with SG, neither factor negatively impacted seedling growth. Both SG and SN_SG produced as many usable plants as Conv; however, seedling height and diameter tended to be lower in SG compared to the other two treatments. No usable transplants were produced when SN was the sole fertility source, likely because of lack of nutrients other than N. Furthermore, many of the organic fertility products require biological activity to mineralize organic N to a plant-available form. This activity can have potentially detrimental outcomes on float system solution pH, dissolved oxygen, and bicarbonate levels.
经过认证的有机烤烟(Nicotiana tabacum L.)的生产在美国经历了显著的扩张。尽管有这种扩大,但很少有信息概述有机氮(N)计划的苗木生产。为了制定种植者建议,研究了秘鲁海鸟鸟粪(SG)、硝酸钠(SN)或两者组合(SN_SG)在浮子系统中对浮子水化学和幼苗活力的影响。常规治疗(Conv;纳入SQM Ultrasol Premium进行比较。2016年6月至2017年1月期间进行了两次温室研究。在移栽植株萌发和生长过程中,对烟草浮水进行两次氮肥处理。浮子系统的水每5天收集一次,分析N形态、pH值、溶解氧和碳酸氢盐。在每次试验结束时,测量移栽尺寸并收集可用植株的百分比。在研究期间,在没有SG的处理中,浮水碳酸氢盐浓度<1 meq L−1,但在播种后(DAS),当SG是唯一的氮源时,浮水碳酸氢盐浓度超过12 meq L−1。尽管SG的铵和碳酸氢盐浓度较高,但这两种因素对幼苗生长都没有负面影响。SG和SN_SG生产的可用植物与Conv一样多;但SG处理的苗高和苗径均低于其他2个处理。当氮是唯一的肥力来源时,没有产生可用的移栽,可能是因为缺乏除氮以外的营养物质。此外,许多有机肥力产品需要生物活性才能将有机氮矿化为植物可利用的形式。这种活性可能会对浮子系统溶液的pH值、溶解氧和碳酸氢盐水平产生潜在的有害影响。
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引用次数: 0
Nicotine Biosynthesis inNicotiana: A Metabolic Overview 烟草中的尼古丁生物合成:代谢综述
Pub Date : 2019-05-10 DOI: 10.3381/18-063
Fernanda F Zenkner, M. Margis-Pinheiro, Alexandro Cagliari
Alkaloids are important compounds found in Nicotiana plants, essential in plant defense against herbivores. The main alkaloid of Nicotiana tabacum, nicotine, is produced in roots and translocated to the leaves. Nicotine is formed by a pyrrolidine and a pyridine ring in a process involving several enzymes. The pyridine ring of nicotine is derived from nicotinic acid, whereas the pyrrolidine ring originates from polyamine putrescine metabolism. After synthesis in root cortical cells, a set of transporters is known to transport nicotine upward to the aerial part and store it in leaf vacuoles. Moreover, nicotine can be metabolized in leaves, giving rise to nornicotine through the N-demethylation process. Some Nicotiana wild species produce acyltransferase enzymes, which allow the plant to make N-acyl-nornicotine, an alkaloid with more potent insecticidal properties than nicotine. However, although we can find a wealth of information about the alkaloid production in Nicotiana spp., our understanding about nicotine biosynthesis, transport, and metabolism is still incomplete. This review will summarize these pathways on the basis on recent literature, as well as highlighting questions that need further investigation.
生物碱是在烟草植物中发现的重要化合物,对植物抵御食草动物至关重要。烟草的主要生物碱尼古丁在根中产生,并转运到叶子中。尼古丁是由一个吡咯烷和一个吡啶环在一个涉及几种酶的过程中形成的。尼古丁的吡啶环来源于烟酸,而吡啶环来源于多胺腐胺代谢。在根皮质细胞中合成尼古丁后,一组转运蛋白将尼古丁向上运输到地上部分并储存在叶液泡中。此外,尼古丁可以在叶片中代谢,通过n -去甲基化过程产生去尼古丁。一些野生烟叶产生酰基转移酶,使植物能够制造n -酰基去烟碱,一种比尼古丁更有效的杀虫特性的生物碱。然而,尽管我们可以发现烟草属生物碱生产的丰富信息,但我们对尼古丁的生物合成、运输和代谢的了解仍然不完整。这篇综述将在最近文献的基础上总结这些途径,并强调需要进一步研究的问题。
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引用次数: 14
VERIFICATION OF NITROGEN AND PHOSPHORUS APPLICATION RATES TO FLUE-CURED TOBACCO 烤烟氮磷肥施用量的验证
Pub Date : 2018-01-12 DOI: 10.3381/17-061
M. Vann, L. Fisher
With rising input costs, flue-cured tobacco producers must consider modern fertility programs that focus on reduced application rates of alternative nutrient sources. To demonstrate the usability o...
随着投入成本的上升,烤烟生产商必须考虑以减少替代营养来源的施用量为重点的现代生育计划。演示的可用性…
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引用次数: 2
CYANTRANILIPROLE AND SPINOSAD RESIDUES IN FLUE-CURED TOBACCO 烤烟中氰氨酰胺和棘糖的残留
Pub Date : 2017-10-11 DOI: 10.3381/17-059
M. Vann, and L.R. Fisher, D. S. Whitley
From 2013 to 2015, research was conducted to estimate the maximum expected residue levels for the insecticides cyantraniliprole and spinosad following application to flue-cured tobacco. Data were generated in order to assist industry in establishing Guidance Residue Limits for both compounds. The insecticides were applied to fields of tobacco at maximum rates in accordance with the labeled rates and the harvested/cured leaf was analyzed in a lab for chemical residues. The findings indicated that the expected residues on cured leaf would be low or not quantifiable under existing detection techniques.
2013 - 2015年,研究了氰硝虫胺和spinosad在烤烟上施用后的最大预期残留水平。生成的数据是为了帮助工业界制定这两种化合物的指导残留限量。在田间按标示的最大用量施用杀虫剂,并在实验室对收获/烘烤后的烟叶进行化学残留分析。结果表明,在现有的检测技术下,烤漆叶片上的预期残留量很低或无法量化。
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引用次数: 1
Analysis of Variability in Curing Conditions and Tobacco-Specific Nitrosamines Within Barns of Dark Air-Cured Tobacco 暗烤烟烤房内烤制条件和烟草特异性亚硝胺的变异分析
Pub Date : 2017-01-01 DOI: 10.3381/17-060
M. Richmond, R. Pearce, B. Goff, W. Bailey
Significant variability in cured-leaf tobacco-specific nitrosamine (TSNA) content is commonly observed when sampling within dark air-curing barns. This variability may be due to inconsistency in th...
在黑暗空气烘烤仓内取样时,通常观察到烤烟特定亚硝胺(TSNA)含量的显著变化。这种可变性可能是由于……
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引用次数: 3
期刊
Chinese Tobacco Science
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