Pub Date : 2018-03-29DOI: 10.25040/ECPB2018.01.039
I. Popovych, V. Babelyuk, T. Korolyshyn, Walery Zukow, C. Moldova
Introduction. It was reported earlier that after two-week course of Intensive Neurophysiological Rehabilitation System (INRS) officially recognized as the Kozyavkin method [18-20], the parameters of the functional tests of hands in 108 children with spastic forms of cerebral palsy (SFCP) have been significantly improved. The increase has been detected in Box and Block Test by 22,9 ± 2,2% right and by 19,1 ± 1,3% left, in Nine Hole Peg Test by 16,7±1,9% right and by 18,8 ± 1,8% left, in Dynamometry by 30,6 ± 5,0% right and by 31,6 ± 6,1% left. In total the effectiveness of the restoration of functional parameters of hands by the Kozyavkin method makes on average 23,3 ± 1,6% versus 3,5 ± 1,4% in control. However, the average indicators obscure the significant differences between individual children. In particular, in 58% of patients, changes are very tangible, in 22% moderate, while in 20% are minor [21]. In another group of 29 children it was found out that reducing of the neural component of muscle tone (NCMT) was stated in 79,3% cases from 7,6 ±1,0 N to 1,6 ± 0,5 N (direct difference: -6,0 ± 0,8 N), while in 13,8% cases changes were not detected and in 2 children only NCMT increased from 1,6 to 3,4 and from 4,6 to 6,1 N respectively [13, 22]. It is known about abnormalities in the autonomous nervous system (ANS) in patients with CP [1, 14, 29]. Obviously, these abnormalities are associated with CNS damage. We have recently discovered the relationships between the parameters of heart rate variability (HRV) as markers of ANS activity and the background of EEG activity [31, 32]. Proceeding from this we hypothesized that such a variety of changes in NCMT is due to ambiguous changes in the background activity of the nerve centers. For their evaluation the following HRV and Electroencephalography (EEG) methods (about Neuroimaging in the conditions of Ukraine we can only dream) are available. Since such children are not always able to register EEG and HRV due to uncontrolled movements, the search for other methods for evaluating neural activity remains relevant. Back in 1880 Nikola Tesla demonstrated that when placing the man in the high-frequency field around the body there is a bright glow. In 1892 Y.O. Nardkevych-Yodko recorded a glow of human hands on a photographic plate. However, a well-known method of ‘high-frequency photography’ was due to the married couple S.D. and V.H. Kirlian who in 1939 independently
{"title":"Relationships between changes in parameters of the manual function and electroencephalogram, heart rate variability as well as gas discharge visualization in children with spastic cerebral palsy caused by the Kozyavkin method","authors":"I. Popovych, V. Babelyuk, T. Korolyshyn, Walery Zukow, C. Moldova","doi":"10.25040/ECPB2018.01.039","DOIUrl":"https://doi.org/10.25040/ECPB2018.01.039","url":null,"abstract":"Introduction. It was reported earlier that after two-week course of Intensive Neurophysiological Rehabilitation System (INRS) officially recognized as the Kozyavkin method [18-20], the parameters of the functional tests of hands in 108 children with spastic forms of cerebral palsy (SFCP) have been significantly improved. The increase has been detected in Box and Block Test by 22,9 ± 2,2% right and by 19,1 ± 1,3% left, in Nine Hole Peg Test by 16,7±1,9% right and by 18,8 ± 1,8% left, in Dynamometry by 30,6 ± 5,0% right and by 31,6 ± 6,1% left. In total the effectiveness of the restoration of functional parameters of hands by the Kozyavkin method makes on average 23,3 ± 1,6% versus 3,5 ± 1,4% in control. However, the average indicators obscure the significant differences between individual children. In particular, in 58% of patients, changes are very tangible, in 22% moderate, while in 20% are minor [21]. In another group of 29 children it was found out that reducing of the neural component of muscle tone (NCMT) was stated in 79,3% cases from 7,6 ±1,0 N to 1,6 ± 0,5 N (direct difference: -6,0 ± 0,8 N), while in 13,8% cases changes were not detected and in 2 children only NCMT increased from 1,6 to 3,4 and from 4,6 to 6,1 N respectively [13, 22]. It is known about abnormalities in the autonomous nervous system (ANS) in patients with CP [1, 14, 29]. Obviously, these abnormalities are associated with CNS damage. We have recently discovered the relationships between the parameters of heart rate variability (HRV) as markers of ANS activity and the background of EEG activity [31, 32]. Proceeding from this we hypothesized that such a variety of changes in NCMT is due to ambiguous changes in the background activity of the nerve centers. For their evaluation the following HRV and Electroencephalography (EEG) methods (about Neuroimaging in the conditions of Ukraine we can only dream) are available. Since such children are not always able to register EEG and HRV due to uncontrolled movements, the search for other methods for evaluating neural activity remains relevant. Back in 1880 Nikola Tesla demonstrated that when placing the man in the high-frequency field around the body there is a bright glow. In 1892 Y.O. Nardkevych-Yodko recorded a glow of human hands on a photographic plate. However, a well-known method of ‘high-frequency photography’ was due to the married couple S.D. and V.H. Kirlian who in 1939 independently","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"30 1","pages":"39-50"},"PeriodicalIF":0.0,"publicationDate":"2018-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73628689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-10DOI: 10.25040/ECPB2018.04.011
N. Babelyuk, Clinical Sanatorium, V. Babelyuk, V. Kikhtan, I. Popovych, M. M. Burkovs’Ka, Y. Dobrovolskyi, I. Korsuns’kyi, Moldova
Introduction. While constructing and creating the generator design for electrotherapy and stimulation of human nerve centers, we based on the following provisions. The influence of impulses of a rectangular shape (range 7-18 Hz) made it possible fix the frequency ranges of each basic nerve node. Low frequency had minimal effects of stimulation on the corresponding nerve node, while high frequency the maximum effect. For the effective excitation of nerve centers, the frequency beat method is used which consists in obtaining oscillations with close frequencies. To obtain the effect of the frequency, beats are generated by impulses of a rectangular shape to two signal channels. The channels differ in frequency which is known to be the beat frequency. For example, for obtaining a beat frequency of 6 Hz, impulses in the first channel were formed at a frequency of 30 Hz, in the second channel at a frequency of 36 Hz. When the first impulse is formed on both channels with a phase shift of 0o, we obtain an absolute zero current in the output. Figure 1 shows a periodic signal generated by frequency beats voltage in two channels to form a common output signal (a). Figure 1 is a graph of the current of the output signal (b). Such effect creates a shock wave through the object at the desired frequency. It also spins an electromagnetic field in the object. The generator has been assembled on the basis of the patent of Ukraine for the utility model 105875 "Portable device for electrotherapy and stimulation" [4]. Its operation is described in [6]. The generator has been assembled on the basis of a two-channel circuit using two frequency synthesizers, amplifiers, each of them generates its own frequency. Figure 2 shows a block diagram of the device indicating the movement of the electric current.
{"title":"The Influence of the Course of Electrostimulation by the Device \"VEB-1\" on Metabolic Parameters of Practically Healthy Men","authors":"N. Babelyuk, Clinical Sanatorium, V. Babelyuk, V. Kikhtan, I. Popovych, M. M. Burkovs’Ka, Y. Dobrovolskyi, I. Korsuns’kyi, Moldova","doi":"10.25040/ECPB2018.04.011","DOIUrl":"https://doi.org/10.25040/ECPB2018.04.011","url":null,"abstract":"Introduction. While constructing and creating the generator design for electrotherapy and stimulation of human nerve centers, we based on the following provisions. The influence of impulses of a rectangular shape (range 7-18 Hz) made it possible fix the frequency ranges of each basic nerve node. Low frequency had minimal effects of stimulation on the corresponding nerve node, while high frequency the maximum effect. For the effective excitation of nerve centers, the frequency beat method is used which consists in obtaining oscillations with close frequencies. To obtain the effect of the frequency, beats are generated by impulses of a rectangular shape to two signal channels. The channels differ in frequency which is known to be the beat frequency. For example, for obtaining a beat frequency of 6 Hz, impulses in the first channel were formed at a frequency of 30 Hz, in the second channel at a frequency of 36 Hz. When the first impulse is formed on both channels with a phase shift of 0o, we obtain an absolute zero current in the output. Figure 1 shows a periodic signal generated by frequency beats voltage in two channels to form a common output signal (a). Figure 1 is a graph of the current of the output signal (b). Such effect creates a shock wave through the object at the desired frequency. It also spins an electromagnetic field in the object. The generator has been assembled on the basis of the patent of Ukraine for the utility model 105875 \"Portable device for electrotherapy and stimulation\" [4]. Its operation is described in [6]. The generator has been assembled on the basis of a two-channel circuit using two frequency synthesizers, amplifiers, each of them generates its own frequency. Figure 2 shows a block diagram of the device indicating the movement of the electric current.","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"39 1","pages":"11-17"},"PeriodicalIF":0.0,"publicationDate":"2018-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86330223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-10DOI: 10.25040/ECPB2018.04.068
O. Motyka, K. Kapustiak, O. Korniychuk
{"title":"Changes of Cultural and Morphological Features and the Formation of the Antibiotic Resistance in Corynebacterium Diphtheriae Induced by Subinhibitory Concentrations of Penicillin","authors":"O. Motyka, K. Kapustiak, O. Korniychuk","doi":"10.25040/ECPB2018.04.068","DOIUrl":"https://doi.org/10.25040/ECPB2018.04.068","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"3 1","pages":"68-73"},"PeriodicalIF":0.0,"publicationDate":"2018-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86962326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-10DOI: 10.25040/ECPB2018.04.035
S. Cherkasov
{"title":"The Influence of the Degree of Impairment of Corticospinal Innervation on the Development of Spinal Hyperexcitability in Patients after Hemispheric Stroke","authors":"S. Cherkasov","doi":"10.25040/ECPB2018.04.035","DOIUrl":"https://doi.org/10.25040/ECPB2018.04.035","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"61 1","pages":"35-40"},"PeriodicalIF":0.0,"publicationDate":"2018-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75837068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-10DOI: 10.25040/ECPB2018.04.062
I. I. Kobza, O. Didenko, O. Yavorskyi, Т. Kobza, Y. Mota
{"title":"The Peculiarities of 24-hour Blood Pressure Monitoring before and after Carotid Endarterectomy in Patients with Arterial Hypertension and Type II Diabetes","authors":"I. I. Kobza, O. Didenko, O. Yavorskyi, Т. Kobza, Y. Mota","doi":"10.25040/ECPB2018.04.062","DOIUrl":"https://doi.org/10.25040/ECPB2018.04.062","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"4 1","pages":"62-67"},"PeriodicalIF":0.0,"publicationDate":"2018-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88830463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-10DOI: 10.25040/ecpb2018.04.024
N. Moskviak, I. Mudra
Background. The assessment of the intellectual development and the hygienic estimation of adaptation have been conducted among the primary schoolchildren of the city of Lviv in connection with the age of starting their education (6 and 7 years old) on the basis of attention and general emotional state estimation by means of the complex test of school-related anxiety that included a number of parameters (the frustration of the need to succeed, the fear of self-ex-pression, the fear of the situation of knowledge testing, the low physiologic stress resilience and issues of fear in the pupil-teacher relationships). Materials and methods. 200 pupils were involved in testing the attention and intellectual development parameters. The estimation of school-related anxiety was done on the basis of tests in which 150 primary schoolchildren took part. Results. According to the results of the intellectual development estimation of the children it has been determined that the number of children with the average intellectual development was higher among the children that started school at the age of 7 (74.5 versus 60.0 % in the younger age group); the number of children with the level of the intellectual development was lower in the older age group (10.9 % versus 14.7 % respectively). The study of attention switching as an ability to get oriented fast in a complex situation and to solve complicated tasks has demonstrated that, according to this parameter, the level of the psychological development of 7 year-olds was higher than their 6 year-old classmates. The reliable difference of the level of school-related anxiety parameters among the studied children of both age groups has shown the more adequate course of adaptation of those pupils who started school education at the age of 7. Conclusion. The obtained results have determined the necessity of the optimization of the course of adaptation of younger pupils to the systematic school activity, especially the 6-year olds, on the basis of psycho-physiological functions and psycho-emotional monitoring status as the objective characteristics of the formation and condition of adaptation.
{"title":"Peculiarities of Сhildren’s Adaptation to the Systematic Educational Activity in Different age Groups","authors":"N. Moskviak, I. Mudra","doi":"10.25040/ecpb2018.04.024","DOIUrl":"https://doi.org/10.25040/ecpb2018.04.024","url":null,"abstract":"Background. The assessment of the intellectual development and the hygienic estimation of adaptation have been conducted among the primary schoolchildren of the city of Lviv in connection with the age of starting their education (6 and 7 years old) on the basis of attention and general emotional state estimation by means of the complex test of school-related anxiety that included a number of parameters (the frustration of the need to succeed, the fear of self-ex-pression, the fear of the situation of knowledge testing, the low physiologic stress resilience and issues of fear in the pupil-teacher relationships). Materials and methods. 200 pupils were involved in testing the attention and intellectual development parameters. The estimation of school-related anxiety was done on the basis of tests in which 150 primary schoolchildren took part. Results. According to the results of the intellectual development estimation of the children it has been determined that the number of children with the average intellectual development was higher among the children that started school at the age of 7 (74.5 versus 60.0 % in the younger age group); the number of children with the level of the intellectual development was lower in the older age group (10.9 % versus 14.7 % respectively). The study of attention switching as an ability to get oriented fast in a complex situation and to solve complicated tasks has demonstrated that, according to this parameter, the level of the psychological development of 7 year-olds was higher than their 6 year-old classmates. The reliable difference of the level of school-related anxiety parameters among the studied children of both age groups has shown the more adequate course of adaptation of those pupils who started school education at the age of 7. Conclusion. The obtained results have determined the necessity of the optimization of the course of adaptation of younger pupils to the systematic school activity, especially the 6-year olds, on the basis of psycho-physiological functions and psycho-emotional monitoring status as the objective characteristics of the formation and condition of adaptation.","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"97 1","pages":"24-30"},"PeriodicalIF":0.0,"publicationDate":"2018-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77079947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-10DOI: 10.25040/ECPB2018.04.041
O. Barvinska, N. Olkhovych, N. Gorovenko
{"title":"Peculiarites of Metabolic Profiles of Amino Acids and Acylcarnitines in Dry Blood Spots of Children with Seizures","authors":"O. Barvinska, N. Olkhovych, N. Gorovenko","doi":"10.25040/ECPB2018.04.041","DOIUrl":"https://doi.org/10.25040/ECPB2018.04.041","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"72 1","pages":"41-47"},"PeriodicalIF":0.0,"publicationDate":"2018-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86155654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-10DOI: 10.25040/ECPB2018.04.048
M. O. Kostіv
{"title":"Changes of the Indicators of Peroxidation of Proteins in Pregnant Women with Diffuse Toxic Goiter","authors":"M. O. Kostіv","doi":"10.25040/ECPB2018.04.048","DOIUrl":"https://doi.org/10.25040/ECPB2018.04.048","url":null,"abstract":"","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"115 1","pages":"48-53"},"PeriodicalIF":0.0,"publicationDate":"2018-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79104298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-10DOI: 10.25040/ECPB2018.04.018
V. Fil, I. Flyunt, O. Mysakovets, O. Mel’nyk, H. Y. Matiyishyn
Background. In the tract of Pomyarky in Truskavets resort the water "Truskavetska", being extracted from the wells, is almost identical to Naftussya with the composition of the electrolytes and the gross organic carbon content, but artificially deprived of microflora. The purpose of this study is to investigate the vegetotropic effect of this water in comparison with the polyphenolic preparation "Enomelanin" as a possible water ameliorator. Material and methods. The experiment was conducted on 48 Wistar rats which were divid-ed into 5 groups of similar sex, body mass, hypoxic and swimming tests. 10 animals of the first group remained relatively intact while others 38 rats received a course of water loads through a tube at a dose 2 % of body mass once daily during seven days. The experimental rats were treated with tap water as a control group, table water "Truskavetska" taken from the bottle as well as "Enomelanin" (10 % paste dissolved in distilled water or ‘Truskavets"ka" water of 0,04 %). One day after the end of the course the ECG was recorded under light ether anesthesia to determine parameters of heart rate variability. Then animals of test groups were subjected to water immersion restraint stress. The day after acute stress the ECG was recorded again. Results. The weekly consumption of ordinary water has not affected the basal stress index at all, whereas water "Truskavetska" has caused a slight tendency to increase it. The water enriched with polyphenols from "Enomelanin" has alleviated this trend. A similar effect has been observed with a 0,04 % solution of "Enomelanin" in distilled water. In the control group after acute stress there has been a tendency to increase the sympathetic tone in combination with a significant reduction in the vagus tone and some sympathetic shift in the humoral channel of regulation. The water "Truskavet'ka" has not affected the latter, but significantly potentiated the sympathetic effect of stress. "Enomelanin" has acted in a similar way. Conclusion. It can be concluded that the vegetotropic effect of polyphenols is conditioned by some factors that accompany the stress reaction.
{"title":"The Сomparative Study of Vegetotropic Effects of Nonpharmacological Agents in Basal and Acute Stress Conditions in Rats of both Sexes","authors":"V. Fil, I. Flyunt, O. Mysakovets, O. Mel’nyk, H. Y. Matiyishyn","doi":"10.25040/ECPB2018.04.018","DOIUrl":"https://doi.org/10.25040/ECPB2018.04.018","url":null,"abstract":"Background. In the tract of Pomyarky in Truskavets resort the water \"Truskavetska\", being extracted from the wells, is almost identical to Naftussya with the composition of the electrolytes and the gross organic carbon content, but artificially deprived of microflora. The purpose of this study is to investigate the vegetotropic effect of this water in comparison with the polyphenolic preparation \"Enomelanin\" as a possible water ameliorator. Material and methods. The experiment was conducted on 48 Wistar rats which were divid-ed into 5 groups of similar sex, body mass, hypoxic and swimming tests. 10 animals of the first group remained relatively intact while others 38 rats received a course of water loads through a tube at a dose 2 % of body mass once daily during seven days. The experimental rats were treated with tap water as a control group, table water \"Truskavetska\" taken from the bottle as well as \"Enomelanin\" (10 % paste dissolved in distilled water or ‘Truskavets\"ka\" water of 0,04 %). One day after the end of the course the ECG was recorded under light ether anesthesia to determine parameters of heart rate variability. Then animals of test groups were subjected to water immersion restraint stress. The day after acute stress the ECG was recorded again. Results. The weekly consumption of ordinary water has not affected the basal stress index at all, whereas water \"Truskavetska\" has caused a slight tendency to increase it. The water enriched with polyphenols from \"Enomelanin\" has alleviated this trend. A similar effect has been observed with a 0,04 % solution of \"Enomelanin\" in distilled water. In the control group after acute stress there has been a tendency to increase the sympathetic tone in combination with a significant reduction in the vagus tone and some sympathetic shift in the humoral channel of regulation. The water \"Truskavet'ka\" has not affected the latter, but significantly potentiated the sympathetic effect of stress. \"Enomelanin\" has acted in a similar way. Conclusion. It can be concluded that the vegetotropic effect of polyphenols is conditioned by some factors that accompany the stress reaction.","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"39 1","pages":"18-23"},"PeriodicalIF":0.0,"publicationDate":"2018-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80596711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-02-10DOI: 10.25040/ECPB2018.04.005
A. Aleksandrov, V. Konopelniuk, O. V. Skopenko, I. Kompanets, L. Ostapchenko
Background. Obesity is one of the most common complex health problems in the world. Main reasons of the development of obesity are marketing the unhealthy food, passive lifestyle, hormonal disorders. Obesity can lead to the development of diabetes mellitus, ischemic heart disease and certain cancers. Numerous neuropeptides and transmitters as well as multiple peripheral hormones take part not only in the regulation of feeding but also in the physical activity. Peptides - serotonin, insulin, glucagon, hormones of hypothalamus and hypophysis, thyroid hormones are the examples of weight regulated hormones. The use of drugs based on steroid hormones, particularly progesterone, can also be one of many factors contributing to obesity. It is known that progesterone which enters the female body artificially leads to fat accumulation. Phenolic biopolymers are the most thoroughly studied natural genesis of macromolecular structures. Melanin occupies a special place among natural polymers. Melanin is chemically relatively inert, but at the same time it is the only polymer with biological properties of a stable free radical. These characteristics determine the structure of their photo- and radioprotective, antioxidant and other properties that is why melanin is widely used in medicine, pharmacology, cosmetology, agriculture and food industry. This article is focused on the molecular and clinical associations between melanin and serum amino acids under progesterone-induced obesity. Serum amino acids are a source for synthesis of hormones that have peptide and protein nature. Revealing these interactions and mechanisms of progesterone-induced obesity may improve our understanding of the complex treatment of obesity. In the current study we used white nonlinear female rats that were divided into three groups. Rats of group 1 – control; group 2 – progesterone-induced obese; group 3 – progesterone-induced obese group under melanin treatment (10 mg/kg body weight, treated daily during 28 days). Amino acid blood serum levels were determined by ion exchange chromatography analyzer (Spekman, Stein, Moore). The statistical analysis of data was carried out by the software package "Statistica 7.0". Values are statistically significant at p < 0.05. Results. The recent study has shown changes in amino acid levels in blood serum of rats under progesterone long-term administration and melanin treatment. Levels of the majority of amino acid content have decreased in rats under progesterone long-term administration and melanin treatment as compared with the control group. The obtained data have given some evidence that progesterone long-term administration has a significant influence on amino acids metabolism. Conclusion. Aromatic amino acids are the substrates for the of neurotransmit- Changes of content of aromatic amino acids can be a of disfunction of the synthesis of these neurotransmitters. The significant decrease of some amino acids, particularly BCAAs and aromatic am
{"title":"Serum Amino Acid Levels in Rats under Long-term Administration of Progesterone and Melanin Treatment","authors":"A. Aleksandrov, V. Konopelniuk, O. V. Skopenko, I. Kompanets, L. Ostapchenko","doi":"10.25040/ECPB2018.04.005","DOIUrl":"https://doi.org/10.25040/ECPB2018.04.005","url":null,"abstract":"Background. Obesity is one of the most common complex health problems in the world. Main reasons of the development of obesity are marketing the unhealthy food, passive lifestyle, hormonal disorders. Obesity can lead to the development of diabetes mellitus, ischemic heart disease and certain cancers. Numerous neuropeptides and transmitters as well as multiple peripheral hormones take part not only in the regulation of feeding but also in the physical activity. Peptides - serotonin, insulin, glucagon, hormones of hypothalamus and hypophysis, thyroid hormones are the examples of weight regulated hormones. The use of drugs based on steroid hormones, particularly progesterone, can also be one of many factors contributing to obesity. It is known that progesterone which enters the female body artificially leads to fat accumulation. Phenolic biopolymers are the most thoroughly studied natural genesis of macromolecular structures. Melanin occupies a special place among natural polymers. Melanin is chemically relatively inert, but at the same time it is the only polymer with biological properties of a stable free radical. These characteristics determine the structure of their photo- and radioprotective, antioxidant and other properties that is why melanin is widely used in medicine, pharmacology, cosmetology, agriculture and food industry. This article is focused on the molecular and clinical associations between melanin and serum amino acids under progesterone-induced obesity. Serum amino acids are a source for synthesis of hormones that have peptide and protein nature. Revealing these interactions and mechanisms of progesterone-induced obesity may improve our understanding of the complex treatment of obesity. In the current study we used white nonlinear female rats that were divided into three groups. Rats of group 1 – control; group 2 – progesterone-induced obese; group 3 – progesterone-induced obese group under melanin treatment (10 mg/kg body weight, treated daily during 28 days). Amino acid blood serum levels were determined by ion exchange chromatography analyzer (Spekman, Stein, Moore). The statistical analysis of data was carried out by the software package \"Statistica 7.0\". Values are statistically significant at p < 0.05. Results. The recent study has shown changes in amino acid levels in blood serum of rats under progesterone long-term administration and melanin treatment. Levels of the majority of amino acid content have decreased in rats under progesterone long-term administration and melanin treatment as compared with the control group. The obtained data have given some evidence that progesterone long-term administration has a significant influence on amino acids metabolism. Conclusion. Aromatic amino acids are the substrates for the of neurotransmit- Changes of content of aromatic amino acids can be a of disfunction of the synthesis of these neurotransmitters. The significant decrease of some amino acids, particularly BCAAs and aromatic am","PeriodicalId":10397,"journal":{"name":"Clinical physiology and biochemistry","volume":"10 1","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"2018-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87598937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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