Background: Radon, a potent carcinogen, is a significant catalyst for lung cancer development. However, the molecular mechanisms triggering radon-induced lung cancer remain elusive.
Methods: Utilizing a radon exposure concentration of 20,000 Bq/m3 for 20 min/session, malignant transformation was induced in human bronchial epithelial cells (BEAS-2B).
Results: Radon-exposed cells derived from passage 25 (BEAS-2B-Rn) exhibited enhanced proliferation and increased colony formation. Analysis of differential gene expression (DEG) through transcription factors revealed 663 up-regulated and 894 down-regulated genes in radon-exposed cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed significant alterations in the malignant transformation pathway of cells, including those related to cancer and the PI3K/AKT signaling pathway. A PPI network analysis indicated a significant association of oncogenes, such as CCND1, KIT, and GATA3, with lung cancer among differentially expressed genes. In addition, the stability of the housekeeping gene was determined through RT-qPCR analysis, which also confirmed the results of transcriptome analysis.
Conclusions: The results suggest that transcription factors may play a pivotal role in conferring a survival advantage to radon-exposed cells. This is achieved by malignant transformation of human bronchial epithelial cells into lung carcinogenesis cell phenotypes.
{"title":"Transcriptomic analysis reveals transcription factors implicated in radon-induced lung carcinogenesis.","authors":"Xing Liu, Yuting Peng, Ruobing Chen, Yueyue Zhou, Xihuan Zou, Mingzhu Xia, Xinyi Wu, Meng Yu","doi":"10.1093/toxres/tfae161","DOIUrl":"10.1093/toxres/tfae161","url":null,"abstract":"<p><strong>Background: </strong>Radon, a potent carcinogen, is a significant catalyst for lung cancer development. However, the molecular mechanisms triggering radon-induced lung cancer remain elusive.</p><p><strong>Methods: </strong>Utilizing a radon exposure concentration of 20,000 Bq/m3 for 20 min/session, malignant transformation was induced in human bronchial epithelial cells (BEAS-2B).</p><p><strong>Results: </strong>Radon-exposed cells derived from passage 25 (BEAS-2B-Rn) exhibited enhanced proliferation and increased colony formation. Analysis of differential gene expression (DEG) through transcription factors revealed 663 up-regulated and 894 down-regulated genes in radon-exposed cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed significant alterations in the malignant transformation pathway of cells, including those related to cancer and the PI3K/AKT signaling pathway. A PPI network analysis indicated a significant association of oncogenes, such as CCND1, KIT, and GATA3, with lung cancer among differentially expressed genes. In addition, the stability of the housekeeping gene was determined through RT-qPCR analysis, which also confirmed the results of transcriptome analysis.</p><p><strong>Conclusions: </strong>The results suggest that transcription factors may play a pivotal role in conferring a survival advantage to radon-exposed cells. This is achieved by malignant transformation of human bronchial epithelial cells into lung carcinogenesis cell phenotypes.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-30eCollection Date: 2024-10-01DOI: 10.1093/toxres/tfae154
Rabab F Hindawy, Rana M M Refaat, Atef E Fouda, Mohamed A El-Shishtawy, Adarsh Kumar, Nagi M El-Shafai, Eman M Faruk, Ola E Nafea
Background: The tobacco epidemic signifies a major public health threat. Nicotine (NIC), a major active constituent in tobacco, impedes male fertility and semen quality. This work is implemented to explore the potential of selenium nanoparticles (SeNPs) and the newly fabricated SeNPs @vitamin C (SeNPs@VITC) nanocomposite in mitigating testicular toxicity induced by NIC.
Materials and methods: The six groups of 48 adult Wistar rats were designed as follows: the control group injected intraperitoneally with normal saline, the SeNPs group treated orally with 2 mg/kg of SeNPs, the SeNPs@VITC nanocomposite group treated orally with 2 mg/kg of SeNPs@VITC nanocomposite, the NIC group injected intraperitoneally with 1.25 mL/kg of NIC, the NIC+ SeNPs group received SeNPs plus NIC, and the NIC+ SeNPs@VITC nanocomposite group received SeNPs@VITC nanocomposite plus NIC. Treatments were administered over a 28-day period.
Results: NIC treatment significantly caused poor sperm quality, decreased serum testosterone, increased follicle-stimulating hormone (FSH), luteinizing hormone (LH) concentrations, reduced hemoglobin levels, leukocytosis, disrupted testicular oxidant/antioxidant balance, and disorganized testicular structure. The construction of the novel SeNPs@VITC nanocomposite, compared to NIC plus SeNPs alone, demonstrated a more potent ameliorative effect on NIC-induced reproductive toxicity in adult rats. The SeNPs@VITC nanocomposite significantly increased sperm count, reduced the percentage of sperm head abnormalities, lowered both serum FSH and LH concentrations, and improved the hemoglobin response.
Conclusions: Both SeNPs and SeNPs@VITC nanocomposite alleviated the testicular toxicity induced by NIC, but the SeNPs@VITC nanocomposite exhibited superior efficacy. The SeNPs@VITC nanocomposite could be employed to advance enhanced therapeutic strategies for addressing male infertility.
{"title":"Exploring the potential of selenium nanoparticles and fabricated selenium nanoparticles @vitamin C nanocomposite in mitigating nicotine-induced testicular toxicity in rats.","authors":"Rabab F Hindawy, Rana M M Refaat, Atef E Fouda, Mohamed A El-Shishtawy, Adarsh Kumar, Nagi M El-Shafai, Eman M Faruk, Ola E Nafea","doi":"10.1093/toxres/tfae154","DOIUrl":"10.1093/toxres/tfae154","url":null,"abstract":"<p><strong>Background: </strong>The tobacco epidemic signifies a major public health threat. Nicotine (NIC), a major active constituent in tobacco, impedes male fertility and semen quality. This work is implemented to explore the potential of selenium nanoparticles (SeNPs) and the newly fabricated SeNPs @vitamin C (SeNPs@VITC) nanocomposite in mitigating testicular toxicity induced by NIC.</p><p><strong>Materials and methods: </strong>The six groups of 48 adult Wistar rats were designed as follows: the control group injected intraperitoneally with normal saline, the SeNPs group treated orally with 2 mg/kg of SeNPs, the SeNPs@VITC nanocomposite group treated orally with 2 mg/kg of SeNPs@VITC nanocomposite, the NIC group injected intraperitoneally with 1.25 mL/kg of NIC, the NIC+ SeNPs group received SeNPs plus NIC, and the NIC+ SeNPs@VITC nanocomposite group received SeNPs@VITC nanocomposite plus NIC. Treatments were administered over a 28-day period.</p><p><strong>Results: </strong>NIC treatment significantly caused poor sperm quality, decreased serum testosterone, increased follicle-stimulating hormone (FSH), luteinizing hormone (LH) concentrations, reduced hemoglobin levels, leukocytosis, disrupted testicular oxidant/antioxidant balance, and disorganized testicular structure. The construction of the novel SeNPs@VITC nanocomposite, compared to NIC plus SeNPs alone, demonstrated a more potent ameliorative effect on NIC-induced reproductive toxicity in adult rats. The SeNPs@VITC nanocomposite significantly increased sperm count, reduced the percentage of sperm head abnormalities, lowered both serum FSH and LH concentrations, and improved the hemoglobin response.</p><p><strong>Conclusions: </strong>Both SeNPs and SeNPs@VITC nanocomposite alleviated the testicular toxicity induced by NIC, but the SeNPs@VITC nanocomposite exhibited superior efficacy. The SeNPs@VITC nanocomposite could be employed to advance enhanced therapeutic strategies for addressing male infertility.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: There is growing concern of the potential damage to vital organs after long term exposure to locally formulated pesticides in rural area of Nigeria. This study was designed to assessed the effects of the individual chemical compound and their combination on the kidney and liver of rats' model.
Methodology: Fifty-four rats divided into six groups and three sub-groups were exposed to 25, 50 and 75% dose of each of the pesticide's LD50 for 4 h at 3 days interval in an inhalation chamber for 28 days. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TOT_BIL), creatinine and urea assay showed significant increase at the aforementioned doses in comparison to the control group. The red blood cell counts, hematocrit and hemoglobin concentrations were significantly altered in the rats administered varying doses of the pesticides when compared with the control. Similar result was obtained for the differential white blood cell counts. Histopathological examinations of the liver tissue of rats showed infiltrated sinusoids, traces of karypyknosis, vacuolar degeneration and microvesicular steatosis while that of the renal tissue showed glomeruli atrophy leading to widened Bowman's spaces as well as few shrunken glomeruli and varied level of degenerative tubular changes to tubular necrosis.
Conclusion: This study established that individual pesticides and their mixture is toxic to the liver and kidney, as evidenced by the elevated markers of renal and liver functions and distortion of the structure of both organs as revealed by their photomicrographs. Therefore, it is a matter of public health significance to regularly monitor pesticide residues in foods and humans in order to assess the food safety risk and population exposure to pesticides.
{"title":"Assessment of the toxic influence of locally formulated pesticides on hepatic and renal biomarkers in male Wistar rats.","authors":"Esther Itunuoluwa Adeyele, Esther Olutomilayo Ayanyemi, Rufus Ojo Akomolafe, Olaoluwa Olukiran Sesan, Omolara Titilayo Aladesanmi, Aderonke Okoya Adetutu","doi":"10.1093/toxres/tfae157","DOIUrl":"10.1093/toxres/tfae157","url":null,"abstract":"<p><strong>Background: </strong>There is growing concern of the potential damage to vital organs after long term exposure to locally formulated pesticides in rural area of Nigeria. This study was designed to assessed the effects of the individual chemical compound and their combination on the kidney and liver of rats' model.</p><p><strong>Methodology: </strong>Fifty-four rats divided into six groups and three sub-groups were exposed to 25, 50 and 75% dose of each of the pesticide's LD<sub>50</sub> for 4 h at 3 days interval in an inhalation chamber for 28 days. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TOT_BIL), creatinine and urea assay showed significant increase at the aforementioned doses in comparison to the control group. The red blood cell counts, hematocrit and hemoglobin concentrations were significantly altered in the rats administered varying doses of the pesticides when compared with the control. Similar result was obtained for the differential white blood cell counts. Histopathological examinations of the liver tissue of rats showed infiltrated sinusoids, traces of karypyknosis, vacuolar degeneration and microvesicular steatosis while that of the renal tissue showed glomeruli atrophy leading to widened Bowman's spaces as well as few shrunken glomeruli and varied level of degenerative tubular changes to tubular necrosis.</p><p><strong>Conclusion: </strong>This study established that individual pesticides and their mixture is toxic to the liver and kidney, as evidenced by the elevated markers of renal and liver functions and distortion of the structure of both organs as revealed by their photomicrographs. Therefore, it is a matter of public health significance to regularly monitor pesticide residues in foods and humans in order to assess the food safety risk and population exposure to pesticides.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was performed to evaluate the therapeutic impact of Diospyros kaki fruit aqueous extract (DKFAE) on ethanol induced peptic ulcer. The phytochemical studies of DKFAE were investigated using colorometric analysis. Gastric ulcer was induced by one dose of ethanol (5 ml/Kg, b.w) on 24 h empty stomach. Then, the plant extract (200, 400 mg/kg) was orally administrated for 2 weeks. Famotidine (FAM: 40 mg/kg, b.w.): a reference drug was also tested. The effect of mixture dose between the fruit extract and FAM (DKFAE, 50 mg/kg PC, p.o. + FAM, 50 mg/kg PC, p.o.) was also evaluated. One hour after induction of ulcer blood samples were collected, stomach acidity and volume, as well as lesion counts were measured, then stomach and intestine of scarified rats were subjected to biochemical, macroscopic and microscopic studies. Results showed that DKFAE exhibited an important antioxidant potential. In vivo, the results showed that alcohol induced gastric damage, improving oxidative stress markers level such as MDA and H2O2, gastric and intestinal calcium and free iron. The intoxication by ethanol also produce an inflammation occurred by high level of the C-reactive protein (CRP) and alkaline phosphatase (ALP) activity in plasma. In contrast, DKFAE and the mixture dose significantly protect against macroscopic and histological injuries, the secretory profile disturbances, lipid peroxidation, antioxidant enzymes activities and non enzymatic antioxidant level decrease induced by ethanol administration. More impressively, the mixture dose exerted the more excellent effect than DKFAE and famotidine each alone showing is possible synergism.
{"title":"<i>Diospyros kaki</i> fruit aqueous extract individual/combined with famotidine mitigates peptic ulcer induced by alcohol in rats.","authors":"Nourhène Dhawefi, Saber Jedidi, Houcem Sammari, Ala Ayari, Mourad Jridi, Hichem Sebai","doi":"10.1093/toxres/tfae155","DOIUrl":"https://doi.org/10.1093/toxres/tfae155","url":null,"abstract":"<p><p>The present study was performed to evaluate the therapeutic impact of <i>Diospyros kaki</i> fruit aqueous extract (DKFAE) on ethanol induced peptic ulcer. The phytochemical studies of DKFAE were investigated using colorometric analysis. Gastric ulcer was induced by one dose of ethanol (5 ml/Kg, b.w) on 24 h empty stomach. Then, the plant extract (200, 400 mg/kg) was orally administrated for 2 weeks. Famotidine (FAM: 40 mg/kg, b.w.): a reference drug was also tested. The effect of mixture dose between the fruit extract and FAM (DKFAE, 50 mg/kg PC, p.o. + FAM, 50 mg/kg PC, p.o.) was also evaluated. One hour after induction of ulcer blood samples were collected, stomach acidity and volume, as well as lesion counts were measured, then stomach and intestine of scarified rats were subjected to biochemical, macroscopic and microscopic studies. Results showed that DKFAE exhibited an important antioxidant potential. In vivo, the results showed that alcohol induced gastric damage, improving oxidative stress markers level such as MDA and H2O2, gastric and intestinal calcium and free iron. The intoxication by ethanol also produce an inflammation occurred by high level of the C-reactive protein (CRP) and alkaline phosphatase (ALP) activity in plasma. In contrast, DKFAE and the mixture dose significantly protect against macroscopic and histological injuries, the secretory profile disturbances, lipid peroxidation, antioxidant enzymes activities and non enzymatic antioxidant level decrease induced by ethanol administration. More impressively, the mixture dose exerted the more excellent effect than DKFAE and famotidine each alone showing is possible synergism.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: A growing number of findings have focused on the distinctive physiochemical characteristics that marine microorganisms have acquired as a result of their adaptation to the challenging conditions inherent in the marine environment. It has been established that the marine environment is a very rich source of bioactive substances with a variety of biological effects and structural diversity. A major discovery was the extraction of xyloketals from Xylaria sp. Numerous thorough studies have subsequently been carried out to determine the medicinal potential of these bioactive components. Xyloketals are thought to be a very promising and significant class of naturally occurring substances with a wide range of potent biological activities, such as radical scavenging, suppression of cell proliferation, reduction of neonatal hypoxic-ischemic brain injury, antioxidant activity, inhibition of acetylcholine esterase, inhibition of L-calcium channels, and others. Xyloketal B is one of the most potent molecules with significant therapeutic properties among the numerous variants discovered.
Conclusion: This review summarizes the structural characterization of all naturally occurring xyloketal compounds, especially the B derivative with an emphasis on their bioactivity and provides an outline of how xyloketals operate in diverse disease scenarios.
背景:越来越多的研究结果关注海洋微生物因适应海洋环境固有的挑战性条件而获得的独特理化特性。已经证实,海洋环境是生物活性物质的一个非常丰富的来源,具有各种生物效应和结构多样性。从木贼中提取木酮素是一项重大发现。随后,人们进行了大量深入研究,以确定这些生物活性成分的药用潜力。木酮醇被认为是一类非常有前途的重要天然物质,具有广泛的强效生物活性,如清除自由基、抑制细胞增殖、减轻新生儿缺氧缺血性脑损伤、抗氧化、抑制乙酰胆碱酯酶、抑制 L-钙通道等。在已发现的众多变体中,木酮醇 B 是最有效的分子之一,具有显著的治疗特性:本综述总结了所有天然木酮化合物的结构特征,尤其是 B 衍生物,重点介绍了它们的生物活性,并概述了木酮类化合物如何在各种疾病中发挥作用。
{"title":"Multifaceted bioactivity of marine fungal derived secondary metabolite, xyloketal B -a review.","authors":"Sreelekshmi Puthuvalnikarthil Udayan, Sini Hariharan, Kottayath Govindan Nevin","doi":"10.1093/toxres/tfae156","DOIUrl":"https://doi.org/10.1093/toxres/tfae156","url":null,"abstract":"<p><strong>Background: </strong>A growing number of findings have focused on the distinctive physiochemical characteristics that marine microorganisms have acquired as a result of their adaptation to the challenging conditions inherent in the marine environment. It has been established that the marine environment is a very rich source of bioactive substances with a variety of biological effects and structural diversity. A major discovery was the extraction of xyloketals from <i>Xylaria</i> sp. Numerous thorough studies have subsequently been carried out to determine the medicinal potential of these bioactive components. Xyloketals are thought to be a very promising and significant class of naturally occurring substances with a wide range of potent biological activities, such as radical scavenging, suppression of cell proliferation, reduction of neonatal hypoxic-ischemic brain injury, antioxidant activity, inhibition of acetylcholine esterase, inhibition of L-calcium channels, and others. Xyloketal B is one of the most potent molecules with significant therapeutic properties among the numerous variants discovered.</p><p><strong>Conclusion: </strong>This review summarizes the structural characterization of all naturally occurring xyloketal compounds, especially the B derivative with an emphasis on their bioactivity and provides an outline of how xyloketals operate in diverse disease scenarios.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11425363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-24eCollection Date: 2024-10-01DOI: 10.1093/toxres/tfae152
Nguyen Quynh Anh Ngo, Huong Thi Nguyen, Xuan Thanh Dam, Dinh Nhi Bui, Thi Thao Minh
This study investigated the acute and repeated 28-day dose toxicity profiles of Bacillus clausii M31, isolated from children's feces, in Swiss rats and New Zealand rabbits. To investigate acute toxicity, rats were given varied doses of B. clausii M31 (1 × 1011 CFU/mL, 3 × 1011 CFU/mL, and 5 × 1011 CFU/mL) orally once daily for 14 days, in accordance with OECD recommendations No. 423. To evaluate toxicity, rabbits were given either a low dosage (1 × 1011 CFU/mL) or a high dose (5 × 1011 CFU/mL) during a 28-day period using the OECD Test Guideline 407 protocol. Neither death nor significant abnormalities were observed in the rats during the experiment. The microscopic examination of key organs revealed no substantial changes in organ morphology. Furthermore, analyses of serum biochemistry and hematological parameters did not reveal any treatment-associated variations. In sum, these findings suggest that the oral intake of B. clausii M31 at concentrations up to 5 × 1011 CFU/mL for 28 days poses no discernible risks.
{"title":"Oral toxicity evaluation of <i>Bacillus clausii</i> M31 isolated from the children's feces in the northern province of Vietnam.","authors":"Nguyen Quynh Anh Ngo, Huong Thi Nguyen, Xuan Thanh Dam, Dinh Nhi Bui, Thi Thao Minh","doi":"10.1093/toxres/tfae152","DOIUrl":"https://doi.org/10.1093/toxres/tfae152","url":null,"abstract":"<p><p>This study investigated the acute and repeated 28-day dose toxicity profiles of <i>Bacillus clausii</i> M31, isolated from children's feces, in Swiss rats and New Zealand rabbits. To investigate acute toxicity, rats were given varied doses of <i>B. clausii</i> M31 (1 × 10<sup>11</sup> CFU/mL, 3 × 10<sup>11</sup> CFU/mL, and 5 × 10<sup>11</sup> CFU/mL) orally once daily for 14 days, in accordance with OECD recommendations No. 423. To evaluate toxicity, rabbits were given either a low dosage (1 × 10<sup>11</sup> CFU/mL) or a high dose (5 × 10<sup>11</sup> CFU/mL) during a 28-day period using the OECD Test Guideline 407 protocol. Neither death nor significant abnormalities were observed in the rats during the experiment. The microscopic examination of key organs revealed no substantial changes in organ morphology. Furthermore, analyses of serum biochemistry and hematological parameters did not reveal any treatment-associated variations. In sum, these findings suggest that the oral intake of <i>B. clausii</i> M31 at concentrations up to 5 × 10<sup>11</sup> CFU/mL for 28 days poses no discernible risks.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Toxicological empirical research suggests that excessive utilization of paraquat, an herbicide, shows detrimental consequences on mammalian reproductive toxicity. The current study aims to study it as a reproductive toxin on the caprine testicular cells at 4- and 6-hour exposure duration. Paraquat treatment decreased the cell viability percentage and induced histological architectural alterations such as disruption of germinal epithelium, vacuolization, and pyknotic nuclei in the testis. The differential EB/AO staining also revealed an increased incidence of apoptosis after paraquat treatment at both dosages, i.e. 10 mM and 100 mM. Paraquat also induces oxidative stress, as evident via increased Malondialdehyde levels (a byproduct of lipid peroxidation) and a decline in the antioxidant capacity (FRAP). However, co-administration of Vitamin E significantly reduced the paraquat-mediated decline in cell viability percentage, histological alterations, and apoptosis incidences and generated oxidative stress, indicating its antioxidative properties against paraquat exposure. This research concludes that Vitamin E co-administration considerably reduced the toxicity of paraquat elicited in testicles, suggesting that Vitamin E may have advantageous potential in preventing the male gonadotoxicity caused by paraquat use in agriculture.
毒理学实证研究表明,过量使用除草剂百草枯会对哺乳动物的生殖毒性产生不利影响。本研究旨在研究百草枯在 4 小时和 6 小时暴露时间内对绒毛动物睾丸细胞产生的生殖毒性。百草枯处理降低了睾丸细胞的存活率,并诱发了组织学结构的改变,如生殖上皮破坏、空泡化和细胞核萎缩。不同的 EB/AO 染色也显示,百草枯处理两种剂量(即 10 毫摩尔和 100 毫摩尔)后,细胞凋亡的发生率均有所增加。百草枯还会诱发氧化应激,表现为丙二醛水平(脂质过氧化的副产物)升高和抗氧化能力(FRAP)下降。然而,同时服用维生素 E 能明显降低百草枯介导的细胞存活率下降、组织学改变、细胞凋亡发生率和氧化应激的产生,这表明维生素 E 对百草枯暴露具有抗氧化特性。这项研究的结论是,联合服用维生素 E 可大大降低百草枯对睾丸的毒性,这表明维生素 E 在预防农业中使用百草枯引起的男性性腺毒性方面可能具有优势。
{"title":"Effective attenuation of Paraquat induced oxidative stress and Genotoxicity in testicular germ cells by vitamin E in Caprines.","authors":"Vishavjeet Rathee, Prerna Bikal, Anshu Siwach, Jitender Kumar Bhardwaj","doi":"10.1093/toxres/tfae153","DOIUrl":"https://doi.org/10.1093/toxres/tfae153","url":null,"abstract":"<p><p>Toxicological empirical research suggests that excessive utilization of paraquat, an herbicide, shows detrimental consequences on mammalian reproductive toxicity. The current study aims to study it as a reproductive toxin on the caprine testicular cells at 4- and 6-hour exposure duration. Paraquat treatment decreased the cell viability percentage and induced histological architectural alterations such as disruption of germinal epithelium, vacuolization, and pyknotic nuclei in the testis. The differential EB/AO staining also revealed an increased incidence of apoptosis after paraquat treatment at both dosages, i.e. 10 mM and 100 mM. Paraquat also induces oxidative stress, as evident via increased Malondialdehyde levels (a byproduct of lipid peroxidation) and a decline in the antioxidant capacity (FRAP). However, co-administration of Vitamin E significantly reduced the paraquat-mediated decline in cell viability percentage, histological alterations, and apoptosis incidences and generated oxidative stress, indicating its antioxidative properties against paraquat exposure. This research concludes that Vitamin E co-administration considerably reduced the toxicity of paraquat elicited in testicles, suggesting that Vitamin E may have advantageous potential in preventing the male gonadotoxicity caused by paraquat use in agriculture.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: The number of new diagnosed cancer cases and cancer deaths are increasing worldwide. Perfluorinated compounds (PFCs) are synthetic chemicals, which are possible inducers of cancer in human and laboratory animals. Studies showed that PFCs induce breast, prostate, kidney, liver and pancreas cancer by inducing genes being involved in carcinogenic pathways.
Methodology: This study reviews the association between PFCs induced up-regulation/down-regulation of genes and signaling pathways that are important in promoting different types of cancer. To obtain chemical-gene interactions, an advanced search was performed in the Comparative Toxicogenomics Database platform.
Results: Five most prevalent cancers were studied and the maps of their signaling pathways were drawn, and colored borders indicate significantly differentially expressed genes if there had been reports of alterations in expression in the presence of PFCs.
Conclusion: In general, PFCs are capable of inducing cancer in human via altering PPARα and PI3K pathways, evading apoptosis, inducing sustained angiogenesis, alterations in proliferation and blocking differentiation. However, more epidemiological data and mechanistic studies are needed to better understand the carcinogenic effects of PFCs in human.
{"title":"A toxicogenomics-based identification of potential mechanisms and signaling pathways involved in PFCs-induced cancer in human.","authors":"Zahra Dehghani, Sara Ranjbar, Farbod Shahabinezhad, Pooria Sabouri, Afshin Mohammadi Bardbori","doi":"10.1093/toxres/tfae151","DOIUrl":"10.1093/toxres/tfae151","url":null,"abstract":"<p><strong>Introduction: </strong>The number of new diagnosed cancer cases and cancer deaths are increasing worldwide. Perfluorinated compounds (PFCs) are synthetic chemicals, which are possible inducers of cancer in human and laboratory animals. Studies showed that PFCs induce breast, prostate, kidney, liver and pancreas cancer by inducing genes being involved in carcinogenic pathways.</p><p><strong>Methodology: </strong>This study reviews the association between PFCs induced up-regulation/down-regulation of genes and signaling pathways that are important in promoting different types of cancer. To obtain chemical-gene interactions, an advanced search was performed in the Comparative Toxicogenomics Database platform.</p><p><strong>Results: </strong>Five most prevalent cancers were studied and the maps of their signaling pathways were drawn, and colored borders indicate significantly differentially expressed genes if there had been reports of alterations in expression in the presence of PFCs.</p><p><strong>Conclusion: </strong>In general, PFCs are capable of inducing cancer in human via altering PPARα and PI3K pathways, evading apoptosis, inducing sustained angiogenesis, alterations in proliferation and blocking differentiation. However, more epidemiological data and mechanistic studies are needed to better understand the carcinogenic effects of PFCs in human.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Prunella vulgaris is widely used as the main ingredient of herb tea in Southeast Asia, as well as a traditional Chinese medicine. However, the heavy metal contaminations such as arsenic, cadmium, mercury and lead in P. vulgaris may be a cause for concern due to the environment pollution around, plantation and processing contamination. Thus, this study intented to assess both non-carcinogenic risks and carcinogenic risks attributed to cumulative exposure to the four heavy metals in P. vulgaris. The contaminations levels of heavy metals were determined in 90 batches of P. vulgaris. And the consumption level was obtained through a questionnaire survey among a total of 6,235 adult participants in Guangdong province. This study estimated the probabilistic health risks using Monte Carlo simulation, and found that the estimated mean and the 95th percentile values for cumulative noncarcinogenic risk (HI value) and carcinogenic risk (TCR value) of P. vulgaris were all within the acceptable risk. And the assessment results indicated that arsenic was the primary contributors to both noncarcinogenic and carcinogenic risks through P. vulgaris consumption. These findings and continuing the surveillance of heavy metals in P. vulgaris will be particularly relevant to both consumers and policy makers.
毛蕊花在东南亚被广泛用作花草茶的主要成分,也是一种传统中药。然而,由于周围环境污染、种植和加工污染,普通樱草中的砷、镉、汞和铅等重金属污染可能会引起人们的关注。因此,本研究旨在评估因累积接触四种重金属而导致的非致癌风险和致癌风险。本研究测定了 90 批 P. vulgaris 的重金属污染水平。通过对广东省 6 235 名成年参与者进行问卷调查,获得了他们的消费水平。该研究采用蒙特卡洛模拟法对健康风险进行了概率估算,结果发现,菠萝的累积非致癌风险(HI 值)和致癌风险(TCR 值)的估算均值和第 95 百分位数均在可接受的风险范围内。评估结果表明,砷是通过食用 P. vulgaris 导致非致癌和致癌风险的主要因素。这些发现以及继续监测茨菰中的重金属对消费者和政策制定者都具有特别重要的意义。
{"title":"Heavy metals and probabilistic risk assessment via <i>Prunella vulgaris</i> (food and medicine homology) consumption in Guangdong Province, China.","authors":"Rui Huang, Shaowei Chen, Ping Wang, Pan Zhu, Xiumin Xu, Zihui Chen, Jiewen Peng","doi":"10.1093/toxres/tfae142","DOIUrl":"https://doi.org/10.1093/toxres/tfae142","url":null,"abstract":"<p><p><i>Prunella vulgaris</i> is widely used as the main ingredient of herb tea in Southeast Asia, as well as a traditional Chinese medicine. However, the heavy metal contaminations such as arsenic, cadmium, mercury and lead in <i>P. vulgaris</i> may be a cause for concern due to the environment pollution around, plantation and processing contamination. Thus, this study intented to assess both non-carcinogenic risks and carcinogenic risks attributed to cumulative exposure to the four heavy metals in <i>P. vulgaris.</i> The contaminations levels of heavy metals were determined in 90 batches of <i>P. vulgaris.</i> And the consumption level was obtained through a questionnaire survey among a total of 6,235 adult participants in Guangdong province. This study estimated the probabilistic health risks using Monte Carlo simulation, and found that the estimated mean and the 95th percentile values for cumulative noncarcinogenic risk (HI value) and carcinogenic risk (TCR value) of <i>P. vulgaris</i> were all within the acceptable risk. And the assessment results indicated that arsenic was the primary contributors to both noncarcinogenic and carcinogenic risks through <i>P. vulgaris</i> consumption. These findings and continuing the surveillance of heavy metals in <i>P. vulgaris</i> will be particularly relevant to both consumers and policy makers.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-23eCollection Date: 2024-10-01DOI: 10.1093/toxres/tfae150
Sautan Show, Debanjan Dutta, Upendra Nongthomba, Mahadesh Prasad A J
Paclitaxel (PCTX) is one of the most prevalently used chemotherapeutic agents. However, its use is currently beset with a host of problems: solubility issue, microplastic leaching, and drug resistance. Since drug discovery is challenging, we decided to focus on repurposing the drug itself by remedying its drawbacks and making it more effective. In this study, we have harnessed the aqueous solubility of sugars, and the high affinity of cancer cells for them, to entrap the hydrophobic PCTX within the hydrophilic shell of the carbohydrate β-cyclodextrin. We have characterized this novel drug formulation by testing its various physical and chemical parameters. Importantly, in all our in vitro assays, the conjugate performed better than the drug alone. We find that the conjugate is internalized by the cancer cells (A549) via caveolin 1-mediated endocytosis. Thereafter, it triggers apoptosis by inducing the formation of reactive oxygen species. Based on experiments on zebrafish larvae, the formulation displays lower toxicity compared to PCTX alone. Thus, our "Trojan Horse" approach, relying on minimal components and relatively faster formulation, enhances the anti-tumor potential of PCTX, while simultaneously making it more innocuous toward non-cancerous cells. The findings of this study have implications in the quest for the most cost-effective chemotherapeutic molecule.
{"title":"Effective paclitaxel: <i>β</i>-Cyclodextrin-based formulation boosts <i>in vitro</i> anti-tumor potential and lowers toxicity in zebrafish.","authors":"Sautan Show, Debanjan Dutta, Upendra Nongthomba, Mahadesh Prasad A J","doi":"10.1093/toxres/tfae150","DOIUrl":"https://doi.org/10.1093/toxres/tfae150","url":null,"abstract":"<p><p>Paclitaxel (PCTX) is one of the most prevalently used chemotherapeutic agents. However, its use is currently beset with a host of problems: solubility issue, microplastic leaching, and drug resistance. Since drug discovery is challenging, we decided to focus on repurposing the drug itself by remedying its drawbacks and making it more effective. In this study, we have harnessed the aqueous solubility of sugars, and the high affinity of cancer cells for them, to entrap the hydrophobic PCTX within the hydrophilic shell of the carbohydrate <i>β</i>-cyclodextrin. We have characterized this novel drug formulation by testing its various physical and chemical parameters. Importantly, in all our <i>in vitro</i> assays, the conjugate performed better than the drug alone. We find that the conjugate is internalized by the cancer cells (A549) via caveolin 1-mediated endocytosis. Thereafter, it triggers apoptosis by inducing the formation of reactive oxygen species. Based on experiments on zebrafish larvae, the formulation displays lower toxicity compared to PCTX alone. Thus, our \"Trojan Horse\" approach, relying on minimal components and relatively faster formulation, enhances the anti-tumor potential of PCTX, while simultaneously making it more innocuous toward non-cancerous cells. The findings of this study have implications in the quest for the most cost-effective chemotherapeutic molecule.</p>","PeriodicalId":105,"journal":{"name":"Toxicology Research","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11417963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142337570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}