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Beyond the Screen-Positive Rate: Racial Equity Considerations for Serum Screening for Open Neural Tube Defects. 超越筛查阳性率:开放性神经管缺陷血清筛查的种族公平考虑。
IF 7.1 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-12-02 DOI: 10.1093/clinchem/hvae143
Christina C Pierre, Dina N Greene, Daniel S Herman, Octavia M Peck Palmer, Shani Delaney
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引用次数: 0
Alkaline Phosphatase Activity Inconsistent with Patient's Clinical Presentation: A Cautionary Tale. 碱性磷酸酶活性与患者临床表现不一致:一个警世故事。
IF 7.1 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-12-02 DOI: 10.1093/clinchem/hvae108
Nicole J Mathewson, Kwaku Baryeh, Joseph W Rudolf, Vishnu Sundaresh, Vrajesh Pandya
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引用次数: 0
Applications of Mass Spectrometry Proteomic Methods to Immunoglobulins in the Clinical Laboratory. 质谱蛋白质组学方法在临床实验室免疫球蛋白检测中的应用。
IF 7.1 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-12-02 DOI: 10.1093/clinchem/hvae179
David L Murray, Maria A V Willrich

Background: Immunoglobulin (Ig) measurements in the clinical laboratory have been traditionally performed by nephelometry, turbidimetry, electrophoresis, and ELISA assays. Mass spectrometry (MS) measurements have the potential to provide deeper insights on the nature of these markers.

Content: Different approaches-top-down, middle-down, or bottom-up-have been described for measuring specific Igs for endogenous monoclonal immunoglobulins (M-proteins) and exogenous therapeutic monoclonal antibody therapies (t-mAbs). Challenges arise in distinguishing the Ig of interest from the polyclonal Ig background. MS is emerging as a practical method to provide quantitative analysis and information about structural and clonal features that are not easily determined by current clinical laboratory methods. This review discusses clinically implemented examples, including isotyping and quantification of M-proteins and quantitation of t-mAbs within the polyclonal Ig background, as examples of how MS can enhance our detection and characterization of Igs.

Summary: This review of current clinically available MS proteomic tests for Igs highlights both analytical and nonanalytical challenges for implementation. Given the new insight into Igs from these methods, it is hoped that vendors, laboratorians, healthcare providers, and payment systems can work to overcome these challenges and advance the care of patients.

背景:在临床实验室中,免疫球蛋白(Ig)的测定传统上是通过浊度法、浊度测定法、电泳法和酶联免疫吸附试验(ELISA)来进行的。质谱(MS)测量有可能更深入地揭示这些标记物的本质:在测量内源性单克隆免疫球蛋白(M-蛋白)和外源性治疗性单克隆抗体疗法(t-mAbs)的特异性 Igs 时,采用了自上而下、自中而下或自下而上的不同方法。从多克隆 Ig 背景中区分相关 Ig 是一项挑战。MS 正在成为一种实用的方法,可提供定量分析以及有关目前临床实验室方法难以确定的结构和克隆特征的信息。本综述讨论了临床应用的实例,包括 M 蛋白的同型和定量以及多克隆 Ig 背景中 t-mAbs 的定量,作为 MS 如何增强 Igs 检测和定性的范例。鉴于这些方法对 Igs 有了新的认识,我们希望供应商、实验室人员、医疗服务提供者和支付系统能够努力克服这些挑战,促进对患者的护理。
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引用次数: 0
Proteomic Signature of BMI and Risk of Cardiovascular Disease. 体重指数与心血管疾病风险的蛋白质组特征。
IF 7.1 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-12-02 DOI: 10.1093/clinchem/hvae149
Hao Ma, Xuan Wang, Yoriko Heianza, JoAnn E Manson, Lu Qi

Background: Obesity, defined by body mass index (BMI) alone, is a metabolically heterogeneous disorder with distinct cardiovascular manifestations across individuals. This study aimed to investigate the associations of a proteomic signature of BMI with risk of major subtypes of cardiovascular disease (CVD).

Methods: A total of 40 089 participants from UK Biobank, free of CVD at baseline, had complete data on proteomic data measured by the Olink assay. A BMI-proteomic score (pro-BMI score) was calculated from 67 pre-identified plasma proteins associated with BMI.

Results: A higher pro-BMI score was significantly associated with higher risks of ischemic heart disease (IHD) and heart failure (HF), but not with risk of stroke. Comparing the highest with the lowest quartiles, the adjusted hazard ratio (HR) for IHD was 1.49 (95% CI, 1.32-1.67) (P-trend < 0.001), and the adjusted HR for HF was 1.52 (95% CI, 1.25-1.85) (P-trend < 0.001). Further analyses showed that the association of pro-BMI score with HF risk was largely driven by the actual BMI, whereas the association of the pro-BMI score with IHD risk was independent of actual BMI and waist-to-hip ratio (WHR). The association between pro-BMI score and IHD risk appeared to be stronger in the normal BMI group than other BMI groups (P-interaction = 0.004) and stronger in the normal WHR group than the high WHR group (P-interaction = 0.049).

Conclusions: Higher pro-BMI score is significantly associated with higher IHD risk, independent of actual BMI levels. Our findings suggest that plasma proteins hold promise as complementary markers for diagnosing obesity and may facilitate personalized interventions.

背景:仅以体重指数(BMI)定义的肥胖症是一种代谢异质性疾病,不同个体的心血管表现各不相同。本研究旨在调查 BMI 蛋白质组特征与心血管疾病(CVD)主要亚型风险的关联:方法:英国生物库中共有 40 089 名基线时未患心血管疾病的参与者,他们的蛋白质组数据均由 Olink 检测法测定。根据与 BMI 相关的 67 种预先确定的血浆蛋白计算出 BMI 蛋白组评分(pro-BMI 评分):结果:pro-BMI 评分越高,患缺血性心脏病(IHD)和心力衰竭(HF)的风险越高,但与中风风险无关。比较最高四分位数和最低四分位数,IHD 的调整后危险比 (HR) 为 1.49(95% CI,1.32-1.67)(P-趋势 < 0.001),HF 的调整后危险比为 1.52(95% CI,1.25-1.85)(P-趋势 < 0.001)。进一步分析表明,pro-BMI 评分与心房颤动风险的关系主要受实际体重指数的影响,而 pro-BMI 评分与心房颤动风险的关系与实际体重指数和腰臀比(WHR)无关。正常体重指数组的pro-BMI评分与IHD风险之间的关联似乎比其他体重指数组更强(P-interaction = 0.004),正常WHR组的pro-BMI评分与IHD风险之间的关联比高WHR组更强(P-interaction = 0.049):结论:较高的 pro-BMI 评分与较高的 IHD 风险显著相关,与实际的 BMI 水平无关。我们的研究结果表明,血浆蛋白有望成为诊断肥胖的补充标记物,并可促进个性化干预。
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引用次数: 0
Forever Chemicals, Endless Testing? Expert Advice to Be Prepared for Per- and Polyfluoroalkyl Substances. 永恒的化学品,无休止的测试?专家建议为全氟和多氟烷基物质做好准备。
IF 7.1 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-12-02 DOI: 10.1093/clinchem/hvae165
Frederick G Strathmann, Susan Burden, Jenna Hua, Andrew Patterson, Robert Middleberg
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引用次数: 0
Commentary on Progressive Motor Regression in a 3-Year-Old: Dietary Trends Revive an Overlooked Diagnosis. 对3岁儿童进行性运动衰退的评论:饮食趋势使一个被忽视的诊断复苏。
IF 7.1 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-12-02 DOI: 10.1093/clinchem/hvae158
Ravinder Sodi
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引用次数: 0
"Fireworks" in Fatty Urine. 脂肪尿中的“烟花”。
IF 7.1 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-12-02 DOI: 10.1093/clinchem/hvae133
Jorik H Amesz, Erika Huijser, Rob F M Bevers, Arjan Albersen
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引用次数: 0
Commentary on Alkaline Phosphatase Activity Inconsistent with Patient's Clinical Presentation: A Cautionary Tale. 碱性磷酸酶活性与患者临床表现不一致的评论:一个警世故事。
IF 7.1 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-12-02 DOI: 10.1093/clinchem/hvae134
Isabelle Piec
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引用次数: 0
Virtual Gene Panels Have a Superior Diagnostic Yield for Inherited Rare Diseases Relative to Static Panels 与静态面板相比,虚拟基因面板对遗传性罕见病的诊断率更高
IF 9.3 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-11-21 DOI: 10.1093/clinchem/hvae183
Massomeh Sheikh Hassani, Ruchi Jain, Sathishkumar Ramaswamy, Shruti Sinha, Maha El Naofal, Nour Halabi, Sawsan Alyafei, Roudha Alfalasi, Shruti Shenbagam, Alan Taylor, Ahmad Abou Tayoun
Background Exome- or genome-based panels—also known as slices or virtual panels—are now a popular approach that involves comprehensive genomic sequencing while restricting analysis to subsets of genes based on patients’ phenotypes. This flexible strategy enables frequent gene updates based on novel disease associations as well as reflexing to analyzing other genes up to the whole exome or genome. With recent improvements addressing limitations associated with virtual panels, the advantages of this approach, relative to static custom-based panels, remain to be systematically characterized. Methods Here we perform slice testing on 1014 patients (50.5% females; average age 17 years) referred from multiple pediatric clinics within a single center in the Middle East (83% Arab population). Results Initial analysis uncovered molecular diagnoses for 235 patients for a diagnostic yield of 23% (235/1014). “On the fly” focused analysis in most negative cases (N = 779) identified clinically significant variants correlating with patients’ presentations in genes outside the originally ordered panel for another 35 patients (3.5% or 35/1024) increasing the overall diagnostic yield to 27%. The pathogenic variants underlying the additional cases (13% of all positive cases) were excluded from the original “panel” gene list, mainly as result of issues related to panel selection, novel gene–disease associations, phenotype spectrum broadening, or gene lists variability. The additional findings led to changes in clinical management in most patients (94%). Conclusions Our findings support slice testing as an efficient and flexible platform that facilitates updates to gene lists to achieve high clinical sensitivity and utility.
背景 基于外显子组或基因组的基因组分析--又称切片分析或虚拟分析--是目前一种流行的方法,它包括全面的基因组测序,同时根据患者的表型限制对基因子集进行分析。这种灵活的策略可根据新的疾病关联频繁更新基因,并可反射性地分析其他基因,直至整个外显子组或基因组。虽然最近的改进解决了与虚拟面板相关的局限性,但相对于静态的定制面板,这种方法的优势仍有待系统鉴定。方法 在这里,我们对中东地区(阿拉伯人口占 83%)一个中心的多个儿科诊所转来的 1014 名患者(50.5% 为女性,平均年龄 17 岁)进行了切片检测。结果 初步分析发现了 235 名患者的分子诊断结果,诊断率为 23%(235/1014)。对大多数阴性病例(N = 779)进行的 "即时 "重点分析发现,另有 35 名患者(3.5% 或 35/1024)的临床重要变异与患者表现相关,这些变异的基因不在最初订购的研究小组范围内,从而将总体诊断率提高到 27%。新增病例(占所有阳性病例的 13%)的致病变异基因被排除在最初的 "面板 "基因列表之外,这主要是由于面板选择、新基因与疾病相关、表型谱扩大或基因列表变异等相关问题造成的。额外的发现导致大多数患者(94%)的临床管理发生了变化。结论 我们的研究结果表明,切片检验是一种高效灵活的平台,可促进基因列表的更新,从而实现较高的临床灵敏度和实用性。
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引用次数: 0
Prospective and External Validation of an Ensemble Learning Approach to Sensitively Detect Intravenous Fluid Contamination in Basic Metabolic Panels 前瞻性外部验证组合学习方法,灵敏检测基础代谢面板中的静脉注射液污染
IF 9.3 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY Pub Date : 2024-11-15 DOI: 10.1093/clinchem/hvae168
Nicholas C Spies, Leah Militello, Christopher W Farnsworth, Joe M El-Khoury, Thomas J S Durant, Mark A Zaydman
Background Intravenous (IV) fluid contamination within clinical specimens causes an operational burden on the laboratory when detected, and potential patient harm when undetected. Even mild contamination is often sufficient to meaningfully alter results across multiple analytes. A recently reported unsupervised learning approach was more sensitive than routine workflows, but still lacked sensitivity to mild but significant contamination. Here, we leverage ensemble learning to more sensitively detect contaminated results using an approach which is explainable and generalizable across institutions. Methods An ensemble-based machine learning pipeline of general and fluid-specific models was trained on real-world and simulated contamination and internally and externally validated. Benchmarks for performance assessment were derived from in silico simulations, in vitro experiments, and expert review. Fluid-specific regression models estimated contamination severity. SHapley Additive exPlanation (SHAP) values were calculated to explain specimen-level predictions, and algorithmic fairness was evaluated by comparing flag rates across demographic and clinical subgroups. Results The sensitivities, specificities, and Matthews correlation coefficients were 0.858, 0.993, and 0.747 for the internal validation set, and 1.00, 0.980, and 0.387 for the external set. SHAP values provided plausible explanations for dextrose- and ketoacidosis-related hyperglycemia. Flag rates from the pipeline were higher than the current workflow, with improved detection of contamination events expected to exceed allowable limits for measurement error and reference change values. Conclusions An accurate, generalizable, and explainable ensemble-based machine learning pipeline was developed and validated for sensitively detecting IV fluid contamination. Implementing this pipeline would help identify errors that are poorly detected by current clinical workflows and a previously described unsupervised machine learning-based method.
背景 临床标本中的静脉注射液(IV)污染一旦被检测到,就会给实验室带来操作负担,而如果未被检测到,则可能对患者造成伤害。即使是轻度污染,也往往足以对多种分析物的检测结果造成有意义的改变。最近报道的一种无监督学习方法比常规工作流程更灵敏,但对轻微但严重的污染仍然缺乏敏感性。在这里,我们利用集合学习来更灵敏地检测污染结果,这种方法可以在不同机构之间进行解释和推广。方法 在真实世界和模拟污染的基础上,对通用模型和特定液体模型进行了基于集合的机器学习管道训练,并进行了内部和外部验证。性能评估基准来自硅学模拟、体外实验和专家评审。特定流体回归模型估计了污染严重程度。通过计算 SHapley Additive exPlanation(SHAP)值来解释标本级预测,并通过比较不同人群和临床亚群的标记率来评估算法的公平性。结果 内部验证集的灵敏度、特异性和马修斯相关系数分别为 0.858、0.993 和 0.747,外部验证集的灵敏度、特异性和马修斯相关系数分别为 1.00、0.980 和 0.387。SHAP 值为葡萄糖和酮症酸中毒相关的高血糖提供了合理的解释。流水线的标记率高于当前工作流程,对超出测量误差和参考变化值允许范围的污染事件的检测能力也有所提高。结论 为灵敏检测静脉输液污染,开发并验证了一种准确、可推广且可解释的基于集合的机器学习管道。实施该管道将有助于识别当前临床工作流程和之前描述的基于无监督机器学习方法难以检测到的错误。
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Clinical chemistry
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