Pub Date : 2025-11-04DOI: 10.1093/clinchem/hvaf096
Jorieke Weiden,Susan D P W M de Jonge-Peeters,Johannes M W van den Ouweland
{"title":"Excess Leading to Deficiency: An Unusual Cause of Cytopenia.","authors":"Jorieke Weiden,Susan D P W M de Jonge-Peeters,Johannes M W van den Ouweland","doi":"10.1093/clinchem/hvaf096","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf096","url":null,"abstract":"","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"28 1","pages":"1105-1108"},"PeriodicalIF":9.3,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145433996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-04DOI: 10.1093/clinchem/hvaf107
Brian D Adkins,Sharon K Germans,Charles F Timmons,Hung S Luu
{"title":"Commentary on Excess Leading to Deficiency: An Unusual Cause of Cytopenia.","authors":"Brian D Adkins,Sharon K Germans,Charles F Timmons,Hung S Luu","doi":"10.1093/clinchem/hvaf107","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf107","url":null,"abstract":"","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"35 1","pages":"1109"},"PeriodicalIF":9.3,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145433992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-04DOI: 10.1093/clinchem/hvaf084
Nicholas C Spies
{"title":"Embracing Generative Artificial Intelligence as a Support Tool for Clinical Decision-Making.","authors":"Nicholas C Spies","doi":"10.1093/clinchem/hvaf084","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf084","url":null,"abstract":"","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"22 1","pages":"1178-1179"},"PeriodicalIF":9.3,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145433994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-04DOI: 10.1093/clinchem/hvaf108
Ravinder Sodi
{"title":"Commentary on Excess Leading to Deficiency: An Unusual Cause of Cytopenia.","authors":"Ravinder Sodi","doi":"10.1093/clinchem/hvaf108","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf108","url":null,"abstract":"","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"88 1","pages":"1110"},"PeriodicalIF":9.3,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145433993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BACKGROUNDDespite almost 3 decades of research, the mechanisms underlying the bidirectional trafficking of cells at the maternal-fetal interface that gives rise to microchimerism remain poorly understood. A major barrier to progress has been the lack of suitable detection methods capable of distinguishing maternal from fetal cells within the spatial context of the human placenta. To address this, we developed a novel detection method based on padlock probe technology to differentiate haploidentical cells in placental tissues.METHODSPadlock probes were designed to target single nucleotide polymorphisms (SNPs) present in messenger RNA transcripts. The assays were first validated in cell lines and subsequently applied to placental tissue to assess its ability to distinguish between maternal and fetal cells.RESULTSWe established a panel of 27 assays targeting 3 human leukocyte antigen-A alleles and 12 biallelic SNPs. The method demonstrated high specificity and sensitivity, detecting minor cell populations at dilutions as low as 1:10 000. Proof of concept was obtained in a decidua basalis specimen, showing the assays' capability to distinguish maternal and fetal cells within placental tissue.CONCLUSIONSWe present a novel, sex-unbiased methodology for the in situ visualization of haploidentical (microchimeric) cells. This approach enables the study of maternal-fetal cellular interactions within their native tissues at the maternal-fetal interface.
{"title":"Single Nucleotide Polymorphism Typing Going Spatial: In Situ Padlock Probes Targeting mRNA Variants to Identify Haploidentical Cells within the Tissue Environment.","authors":"Emiel Slaats,Katharina Schuch,Katja Sallinger,Julia Schönberger,Bernadette Luise Bramreiter,Jaqueline Anholts,Daniel Pitz Jacobsen,Anne Cathrine Staff,Amin El-Heliebi,Michael Eikmans,Thomas Kroneis","doi":"10.1093/clinchem/hvaf119","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf119","url":null,"abstract":"BACKGROUNDDespite almost 3 decades of research, the mechanisms underlying the bidirectional trafficking of cells at the maternal-fetal interface that gives rise to microchimerism remain poorly understood. A major barrier to progress has been the lack of suitable detection methods capable of distinguishing maternal from fetal cells within the spatial context of the human placenta. To address this, we developed a novel detection method based on padlock probe technology to differentiate haploidentical cells in placental tissues.METHODSPadlock probes were designed to target single nucleotide polymorphisms (SNPs) present in messenger RNA transcripts. The assays were first validated in cell lines and subsequently applied to placental tissue to assess its ability to distinguish between maternal and fetal cells.RESULTSWe established a panel of 27 assays targeting 3 human leukocyte antigen-A alleles and 12 biallelic SNPs. The method demonstrated high specificity and sensitivity, detecting minor cell populations at dilutions as low as 1:10 000. Proof of concept was obtained in a decidua basalis specimen, showing the assays' capability to distinguish maternal and fetal cells within placental tissue.CONCLUSIONSWe present a novel, sex-unbiased methodology for the in situ visualization of haploidentical (microchimeric) cells. This approach enables the study of maternal-fetal cellular interactions within their native tissues at the maternal-fetal interface.","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"90 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145440691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-04DOI: 10.1093/clinchem/hvaf037
Simona Ferraro, Simona Da Molin, Cristina Cereda, Gianvincenzo Zuccotti, Santica Marcovina, Bruno Mario Cesana
{"title":"Vitamin B12 Deficiency and New Recommendations by the National Institute for Health and Care Excellence: A Challenging Clinical and Laboratory Topic.","authors":"Simona Ferraro, Simona Da Molin, Cristina Cereda, Gianvincenzo Zuccotti, Santica Marcovina, Bruno Mario Cesana","doi":"10.1093/clinchem/hvaf037","DOIUrl":"10.1093/clinchem/hvaf037","url":null,"abstract":"","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":" ","pages":"1102-1104"},"PeriodicalIF":6.3,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-04DOI: 10.1093/clinchem/hvaf112
{"title":"Correction to: In Vitro Metabolic Profiling of 18 Semi-Synthetic Cannabinoids-Hexahydrocannabinol (HHC) and Its Analogs-with Identification in an Authentic Hexahydrocannabiphorol (HHCP) Urine Sample.","authors":"","doi":"10.1093/clinchem/hvaf112","DOIUrl":"10.1093/clinchem/hvaf112","url":null,"abstract":"","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":" ","pages":"1180"},"PeriodicalIF":6.3,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-23DOI: 10.1093/clinchem/hvaf111
Fabienne Lucas,Sindhu Cherian,Michael A Linden,Mehrnoosh Tashakori
BACKGROUNDFlow cytometry is a crucial diagnostic tool in hematopathology. It supports the identification and follow-up of hematolymphoid neoplasms by providing detailed immunophenotypic data and quantification, and is applicable to various specimen types.CONTENTClassification and management of acute myeloid leukemia (AML), the most common type of acute leukemia in adults, are complex and evolving, guided by International Consensus Classification and World Health Organization 5th edition diagnostic frameworks, as well as the clinically oriented European LeukemiaNet recommendations. These systems stratify AML based on genomic, morphologic, and immunophenotypic features, informing treatment and outcomes. While cytogenetic and molecular data are central to subclassification, flow cytometry remains essential for rapid diagnostic and prognostic insights. This review explores the role of flow cytometry in diagnosing AML, monitoring disease progression, and assessing measurable residual disease, a key relapse and/or outcome predictor. It details how flow cytometry establishes cell lineage, identifies immunophenotypic markers, and predicts genetic alterations in certain subtypes of AML. Examples of analysis strategies and common flow cytometry patterns, as well as references to in-depth recent review articles on the biology, diagnosis and management of AML in the broader context of myeloid malignancies are provided.SUMMARYFlow cytometry is indispensable in the diagnosis, classification, and monitoring of AML. Its ability to provide precise, quantitative information enables early and accurate diagnosis, optimizes treatment, and enhances posttherapy monitoring. Although sophisticated flow cytometry-based approaches may be limited to specialized laboratories, laboratory medicine professionals benefit from a thorough understanding of this technology.
{"title":"Flow Cytometry in Acute Myeloid Leukemia (AML): A Critical Tool for Accurate Diagnosis, Classification, and Monitoring.","authors":"Fabienne Lucas,Sindhu Cherian,Michael A Linden,Mehrnoosh Tashakori","doi":"10.1093/clinchem/hvaf111","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf111","url":null,"abstract":"BACKGROUNDFlow cytometry is a crucial diagnostic tool in hematopathology. It supports the identification and follow-up of hematolymphoid neoplasms by providing detailed immunophenotypic data and quantification, and is applicable to various specimen types.CONTENTClassification and management of acute myeloid leukemia (AML), the most common type of acute leukemia in adults, are complex and evolving, guided by International Consensus Classification and World Health Organization 5th edition diagnostic frameworks, as well as the clinically oriented European LeukemiaNet recommendations. These systems stratify AML based on genomic, morphologic, and immunophenotypic features, informing treatment and outcomes. While cytogenetic and molecular data are central to subclassification, flow cytometry remains essential for rapid diagnostic and prognostic insights. This review explores the role of flow cytometry in diagnosing AML, monitoring disease progression, and assessing measurable residual disease, a key relapse and/or outcome predictor. It details how flow cytometry establishes cell lineage, identifies immunophenotypic markers, and predicts genetic alterations in certain subtypes of AML. Examples of analysis strategies and common flow cytometry patterns, as well as references to in-depth recent review articles on the biology, diagnosis and management of AML in the broader context of myeloid malignancies are provided.SUMMARYFlow cytometry is indispensable in the diagnosis, classification, and monitoring of AML. Its ability to provide precise, quantitative information enables early and accurate diagnosis, optimizes treatment, and enhances posttherapy monitoring. Although sophisticated flow cytometry-based approaches may be limited to specialized laboratories, laboratory medicine professionals benefit from a thorough understanding of this technology.","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"52 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145351701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-22DOI: 10.1093/clinchem/hvaf126
Wayne Hall,Johannes G Ramaekers
{"title":"Challenges in Responding to Cannabis-Impaired Motor Vehicle Drivers.","authors":"Wayne Hall,Johannes G Ramaekers","doi":"10.1093/clinchem/hvaf126","DOIUrl":"https://doi.org/10.1093/clinchem/hvaf126","url":null,"abstract":"","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"51 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2025-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145339018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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