Objective : To investigate the effect of Cupping Therapy at a patho-physiological level for anterior knee pain and its impact on Quality of life and well-being. Method: Experimental survey utilising clinical trial and a questionnaire. A three week follow-up was conducted to determine longer term carry over of treatment effects utilising both objective and subjective assessment. This method enables the researcher to examine how much the independent variable causes participants to change (Dane, 1990). Results: There was statistically significance difference between the level of pain, well being and Range of Motion for patients with anterior knee pain pre and post Cupping (P <0.05). Conclusions : The efficacy of the treatment of Cupping Therapy for Anterior Knee Pain, well being and range of motion has been researched and results reveal improvements in participants as a result of Cupping Therapy. It is recommended that further studies are conducted with larger study samples and of longer duration.
{"title":"An investigation into the effect of Cupping Therapy as a treatment for Anterior Knee Pain and its potential role in Health Promotion.","authors":"K. Ullah, Ahmed Younis Ibrahim Younis, M. Wali","doi":"10.5580/796","DOIUrl":"https://doi.org/10.5580/796","url":null,"abstract":"Objective : To investigate the effect of Cupping Therapy at a patho-physiological level for anterior knee pain and its impact on Quality of life and well-being. Method: Experimental survey utilising clinical trial and a questionnaire. A three week follow-up was conducted to determine longer term carry over of treatment effects utilising both objective and subjective assessment. This method enables the researcher to examine how much the independent variable causes participants to change (Dane, 1990). Results: There was statistically significance difference between the level of pain, well being and Range of Motion for patients with anterior knee pain pre and post Cupping (P <0.05). Conclusions : The efficacy of the treatment of Cupping Therapy for Anterior Knee Pain, well being and range of motion has been researched and results reveal improvements in participants as a result of Cupping Therapy. It is recommended that further studies are conducted with larger study samples and of longer duration.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122693733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Complementary and alternative medicine use in adults with, or at risk for, osteopenia is common. Although most of the herbs and supplements appear to be fairly safe, there is insufficient evidence that demonstrates their beneficial effects. Aim of the Study: This study was done to determine whether the current nutrients improve indicators of calcium metabolism and bone status. Materials and Methods: This human clinical trial was randomized, double-blind, placebo-controlled, and prospective in design. Of a population of 72 individuals who were screened over the telephone, 47 post-menopausal females age 50-75 were included in the study. The enrolled subjects were randomly assigned to receive the active product or placebo, one capsule four times per day for six weeks. The major outcome variables were 24-hour urinary calcium per gram of creatinine, serum Bone Specific Alkaline Phosphatase (BSAP), C-Terminal Telopeptide (CTX), and Osteocalcin (OST). Results: At the conclusion of the study, subjects who received the active product showed reduction in the 24-hour urinary calcium loss and serum CTX levels. They revealed an increase in BSAP and no change was noted for OST. Conclusions: Nutrient-based supplementation is able to improve measures of calcium metabolism and bone health in postmenopausal females. Studies of longer duration using endpoints including fracture incidence and bone densitometry should be conducted in the future.
{"title":"Assessment Of A Nutrient-Based Approach On Bone Health","authors":"J. Blum","doi":"10.5580/1770","DOIUrl":"https://doi.org/10.5580/1770","url":null,"abstract":"Background: Complementary and alternative medicine use in adults with, or at risk for, osteopenia is common. Although most of the herbs and supplements appear to be fairly safe, there is insufficient evidence that demonstrates their beneficial effects. Aim of the Study: This study was done to determine whether the current nutrients improve indicators of calcium metabolism and bone status. Materials and Methods: This human clinical trial was randomized, double-blind, placebo-controlled, and prospective in design. Of a population of 72 individuals who were screened over the telephone, 47 post-menopausal females age 50-75 were included in the study. The enrolled subjects were randomly assigned to receive the active product or placebo, one capsule four times per day for six weeks. The major outcome variables were 24-hour urinary calcium per gram of creatinine, serum Bone Specific Alkaline Phosphatase (BSAP), C-Terminal Telopeptide (CTX), and Osteocalcin (OST). Results: At the conclusion of the study, subjects who received the active product showed reduction in the 24-hour urinary calcium loss and serum CTX levels. They revealed an increase in BSAP and no change was noted for OST. Conclusions: Nutrient-based supplementation is able to improve measures of calcium metabolism and bone health in postmenopausal females. Studies of longer duration using endpoints including fracture incidence and bone densitometry should be conducted in the future.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129207045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Olamide, A. Kayode, A. David, O. Idowu, U. O. Christina, O. Victor, A. A. Rotimi
The objective of this study was to evaluate testicular and epididymal alterations resulting from the administration of ethanolic extract of Garcinia cambogia by morphometric methods. Fifteen (15) rats weighing between 120-135g were used for the study. These were divided into control and experimental groups of 5 rats each. They were given ethanolic extract at doses of 0.00mg/kg B.W (control), 100mg/kg B.W (Group B), 200mg/kg B.W (Group C) respectively by gastric lavage for 6 weeks. Five slides chosen at random from the testicular slides of control and experimental were evaluated and analyzed. There was a reduction in the germinative cell thickness of the seminiferous tubules in the treated group compare with control. There was a significant increase in the sperm counts but reduction in motility in the treated groups in a dose dependent manner compare with control (P<0.05). The volume density ratio of lumen was increased in the treated groups which receive the higher dose of extract compared with the control.
{"title":"Micromorphometric and Stereological Effects of Ethanolic Extracts of Garcinia cambogia seeds on the Testes and Epididymides of Adult Wistar Rats","authors":"A. Olamide, A. Kayode, A. David, O. Idowu, U. O. Christina, O. Victor, A. A. Rotimi","doi":"10.5580/833","DOIUrl":"https://doi.org/10.5580/833","url":null,"abstract":"The objective of this study was to evaluate testicular and epididymal alterations resulting from the administration of ethanolic extract of Garcinia cambogia by morphometric methods. Fifteen (15) rats weighing between 120-135g were used for the study. These were divided into control and experimental groups of 5 rats each. They were given ethanolic extract at doses of 0.00mg/kg B.W (control), 100mg/kg B.W (Group B), 200mg/kg B.W (Group C) respectively by gastric lavage for 6 weeks. Five slides chosen at random from the testicular slides of control and experimental were evaluated and analyzed. There was a reduction in the germinative cell thickness of the seminiferous tubules in the treated group compare with control. There was a significant increase in the sperm counts but reduction in motility in the treated groups in a dose dependent manner compare with control (P<0.05). The volume density ratio of lumen was increased in the treated groups which receive the higher dose of extract compared with the control.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114199470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. G. Souza, Raghuvir Singh, P. P. Reddy, V. I. Hukkeri, V. Byahatti
Purpose: To evaluate aqueous and alcoholic extract of fruit pulp of Litchi chinensis for hepatoprotective activity on carbon tetrachloride induced hepatotoxicity in rats Methodology: Fruit pulp of Litchi chinensis was pulverized, first batch was extracted with alcohol (90% v/v) and second batch was extracted with distilled water. Both the extracts were concentrated and dried separately under vacuum. Extracts were screened for hepatoprotective activity using albino rats (250-300gms) of either sex. Control group was treated with normal saline. Hepatotoxicity was induced by administering carbon tetrachloride, LIV-52 a marketed product was taken as standard and other groups were treated with alcoholic and aqueous extracts. After nine days the serum was analyzed for Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxalate Transaminase (SGOT), Alkaline Phosphatase (ALP) and serum bilirubin. Livers were isolated, weighed and subjected for histopathological studies. Results: Carbon tetrachloride administration in rats elevated the level of SGPT, SGOT, ALP and bilirubin. Administration of LIV-52, alcoholic and aqueous extract significantly prevented this increase. Aqueous extract was found to be more effective than the alcoholic extract. Histopathological studies also confirmed the above investigation. Conclusion: Both alcoholic and aqueous extract of fruit pulp of Lichi chinensis has shown significant (p<0.05) hepatoprotective activity in carbon tetrachloride induced hepatotoxicity and aqueous extract is found to be more effective than the alcoholic extract.
{"title":"Hepatoprotective Activity of Fruit Pulp Extract of Litchi chinensis Sonner on Carbon tetrachloride Induced Hepatotoxicity in albino Rats","authors":"M. G. Souza, Raghuvir Singh, P. P. Reddy, V. I. Hukkeri, V. Byahatti","doi":"10.5580/570","DOIUrl":"https://doi.org/10.5580/570","url":null,"abstract":"Purpose: To evaluate aqueous and alcoholic extract of fruit pulp of Litchi chinensis for hepatoprotective activity on carbon tetrachloride induced hepatotoxicity in rats Methodology: Fruit pulp of Litchi chinensis was pulverized, first batch was extracted with alcohol (90% v/v) and second batch was extracted with distilled water. Both the extracts were concentrated and dried separately under vacuum. Extracts were screened for hepatoprotective activity using albino rats (250-300gms) of either sex. Control group was treated with normal saline. Hepatotoxicity was induced by administering carbon tetrachloride, LIV-52 a marketed product was taken as standard and other groups were treated with alcoholic and aqueous extracts. After nine days the serum was analyzed for Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxalate Transaminase (SGOT), Alkaline Phosphatase (ALP) and serum bilirubin. Livers were isolated, weighed and subjected for histopathological studies. Results: Carbon tetrachloride administration in rats elevated the level of SGPT, SGOT, ALP and bilirubin. Administration of LIV-52, alcoholic and aqueous extract significantly prevented this increase. Aqueous extract was found to be more effective than the alcoholic extract. Histopathological studies also confirmed the above investigation. Conclusion: Both alcoholic and aqueous extract of fruit pulp of Lichi chinensis has shown significant (p<0.05) hepatoprotective activity in carbon tetrachloride induced hepatotoxicity and aqueous extract is found to be more effective than the alcoholic extract.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114934395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laser Doppler flowmetry as well as laser Doppler imaging and thermal imaging play an important role in the scientific of peripheral effects of acupuncture. Microcirculation and temperature monitoring was performed in four healthy volunteers (mean age SD: 33.5 11.4 years; 3 female, 1 male) prior to, during and after insertion and stimulation of an acupuncture needle at the acupoint Jiexi (St.41). The average skin perfusion (Flux) increased significantly at the recording position Zhiyin (UB.67). This was not the case in a control point. It is supposed that similar effects are present as during stimulation of the acupoint Neiguan (Pe.6) at the wrist of the hand which showed a marked increase after 1 minute in microcirculation at the fingertip.
{"title":"Microcirculation And Temperature Monitoring During Acupuncture On Jiexi (St.41)","authors":"G. Litscher, Lu Wang, Ingrid Gaischek","doi":"10.5580/1844","DOIUrl":"https://doi.org/10.5580/1844","url":null,"abstract":"Laser Doppler flowmetry as well as laser Doppler imaging and thermal imaging play an important role in the scientific of peripheral effects of acupuncture. Microcirculation and temperature monitoring was performed in four healthy volunteers (mean age SD: 33.5 11.4 years; 3 female, 1 male) prior to, during and after insertion and stimulation of an acupuncture needle at the acupoint Jiexi (St.41). The average skin perfusion (Flux) increased significantly at the recording position Zhiyin (UB.67). This was not the case in a control point. It is supposed that similar effects are present as during stimulation of the acupoint Neiguan (Pe.6) at the wrist of the hand which showed a marked increase after 1 minute in microcirculation at the fingertip.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133736756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years, research on medicinal plants has attracted a lot of attention globally. Large body of evidence has accumulated to demonstrate promising potential of medicinal plants used in various traditional, complementary and alternative systems. Several Indian medicinal plants have been studied for pharmacological activity in recent years. To understand the mechanism of action, the researchers have worked at molecular levels and several significant phytochemicals have been isolated. The present review is aimed at compiling data on promising phytochemicals from Indian medicinal plants that have been tested in various disease models using modern scientific methodologies and tools.
{"title":"A Review of Pharmacology of Phytochemicals from Indian Medicinal Plants","authors":"S. Malhotra, A. Singh","doi":"10.5580/75c","DOIUrl":"https://doi.org/10.5580/75c","url":null,"abstract":"In recent years, research on medicinal plants has attracted a lot of attention globally. Large body of evidence has accumulated to demonstrate promising potential of medicinal plants used in various traditional, complementary and alternative systems. Several Indian medicinal plants have been studied for pharmacological activity in recent years. To understand the mechanism of action, the researchers have worked at molecular levels and several significant phytochemicals have been isolated. The present review is aimed at compiling data on promising phytochemicals from Indian medicinal plants that have been tested in various disease models using modern scientific methodologies and tools.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132588930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: At the moment generally accepted proof for meridians in Traditional Chinese Medicine (TCM) cannot be considered as being given. Goal of this study was to examine whether possible stimulation-induced meridian-like structures, as recently described by other authors, can be visualized and objectified in dead human subjects using infrared thermography. Methods: Two infrared cameras at different wavelength ranges (2 5 μm and 7.5 13 μm) were used. To validate the method described by other authors, investigations in two dead subjects (72 and 84 years) were performed. Results: After moxibustion of the body, different structures appear on thermographic images of the human body which are technical artifacts and which are not identical to what are known as meridians in TCM. Conclusion: The findings raise questions about the validity of the method described by other authors. Further studies in alive human subjects are necessary regarding the possible visualization of meridians.
{"title":"\"Meridian-like channels\" in dead human subjects?: A negative result","authors":"G. Litscher","doi":"10.5580/61e","DOIUrl":"https://doi.org/10.5580/61e","url":null,"abstract":"Background: At the moment generally accepted proof for meridians in Traditional Chinese Medicine (TCM) cannot be considered as being given. Goal of this study was to examine whether possible stimulation-induced meridian-like structures, as recently described by other authors, can be visualized and objectified in dead human subjects using infrared thermography. Methods: Two infrared cameras at different wavelength ranges (2 5 μm and 7.5 13 μm) were used. To validate the method described by other authors, investigations in two dead subjects (72 and 84 years) were performed. Results: After moxibustion of the body, different structures appear on thermographic images of the human body which are technical artifacts and which are not identical to what are known as meridians in TCM. Conclusion: The findings raise questions about the validity of the method described by other authors. Further studies in alive human subjects are necessary regarding the possible visualization of meridians.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130430218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Attention deficit hyperactivity disorder (ADHD) is a neurobiological disorder of self-control. It consists of problems with attention span, impulse control, and activity level. Depression is one of the mental, emotional, and behavior disorders that can appear during childhood and adolescence. Herbal medicines used for ADHD and depression are Brahmi, Ashwaganda, Ginkgo biloba, and St. John's wart. Questionnaire survey is conducted among 100 doctors (60 children's psychiatrists and 40 pediatricians) from mental health centers and hospitals in Karnataka , 50 pharmacists and 100 caregivers of children (70 Parents and 30 primary caregivers) to analyze whether herbal medicines were given to children receiving care for ADHD and depression. The main outcome measure was doctors and pharmacist view and caregivers self-report of the use of herbal therapy in their children. It was found from survey report that lifetime prevalence of herbal therapy in patients was 20% (20 patients). Eighteen patients (15%) had taken herbal medicines during the past year. Recommendations from a friend or relative resulted in the administration of herbal medicines by 61% of 23 caregivers. Gereforte and Mentat from Himalaya drugs are commonly used for ADHD and depression Almost 83% of caregivers gave herbal medicines alone, whereas 13% gave herbal medicines with prescription drugs. Most caregivers (78%) supervised the administration of herbal therapy in their children; the children's psychiatrists (70%), pediatricians (56%), or pharmacists (74%) typically were not aware of the use. Most caregivers supervised herbal therapy in their children, without communication with a health professional. A need exists for better communication between health professionals and parents or caregivers regarding the use of herbal therapy. THE SOURCE OF ANY SUPPORT RECEIVED Department of Pharmaceutical Marketing and Management, K.L.E.S' College of Pharmacy, Vidyanagar, Hubli580031, Karnataka, India. INTRODUCTION Attention deficit hyperactivity disorder (ADHD) is a neurobiological disorder of self-control. It consists of problems with attention span, impulse control, and activity level. Some of the factors responsible for ADHD in children include drug use during pregnancy, genetics, refined sugar or food additives, brain injury and toxins1. Behaviors associated with ADHD includeTrouble finishing daily tasks, Listening problems, Distracted easily, Difficulties finishing school work, Needing much supervision, Difficulty waiting in line for group activities, Excessively running and Climbing Difficulty sitting and staying seated8. Depression is a serious disorder that can cause significant problems in mood, thinking, and behavior at home, in school, and with peers2. Depressive disorders come in different forms, just as is the case with other illnesses such as heart disease. This pamphlet briefly describes three of the most common types of depressive disorders. However, within these types there are variat
{"title":"Herbal Therapy: Children With Adhd (Attention Deficit Hyperactivity Disorder) And Depression","authors":"P. P. Reddy, S. Devi","doi":"10.5580/47b","DOIUrl":"https://doi.org/10.5580/47b","url":null,"abstract":"Attention deficit hyperactivity disorder (ADHD) is a neurobiological disorder of self-control. It consists of problems with attention span, impulse control, and activity level. Depression is one of the mental, emotional, and behavior disorders that can appear during childhood and adolescence. Herbal medicines used for ADHD and depression are Brahmi, Ashwaganda, Ginkgo biloba, and St. John's wart. Questionnaire survey is conducted among 100 doctors (60 children's psychiatrists and 40 pediatricians) from mental health centers and hospitals in Karnataka , 50 pharmacists and 100 caregivers of children (70 Parents and 30 primary caregivers) to analyze whether herbal medicines were given to children receiving care for ADHD and depression. The main outcome measure was doctors and pharmacist view and caregivers self-report of the use of herbal therapy in their children. It was found from survey report that lifetime prevalence of herbal therapy in patients was 20% (20 patients). Eighteen patients (15%) had taken herbal medicines during the past year. Recommendations from a friend or relative resulted in the administration of herbal medicines by 61% of 23 caregivers. Gereforte and Mentat from Himalaya drugs are commonly used for ADHD and depression Almost 83% of caregivers gave herbal medicines alone, whereas 13% gave herbal medicines with prescription drugs. Most caregivers (78%) supervised the administration of herbal therapy in their children; the children's psychiatrists (70%), pediatricians (56%), or pharmacists (74%) typically were not aware of the use. Most caregivers supervised herbal therapy in their children, without communication with a health professional. A need exists for better communication between health professionals and parents or caregivers regarding the use of herbal therapy. THE SOURCE OF ANY SUPPORT RECEIVED Department of Pharmaceutical Marketing and Management, K.L.E.S' College of Pharmacy, Vidyanagar, Hubli580031, Karnataka, India. INTRODUCTION Attention deficit hyperactivity disorder (ADHD) is a neurobiological disorder of self-control. It consists of problems with attention span, impulse control, and activity level. Some of the factors responsible for ADHD in children include drug use during pregnancy, genetics, refined sugar or food additives, brain injury and toxins1. Behaviors associated with ADHD includeTrouble finishing daily tasks, Listening problems, Distracted easily, Difficulties finishing school work, Needing much supervision, Difficulty waiting in line for group activities, Excessively running and Climbing Difficulty sitting and staying seated8. Depression is a serious disorder that can cause significant problems in mood, thinking, and behavior at home, in school, and with peers2. Depressive disorders come in different forms, just as is the case with other illnesses such as heart disease. This pamphlet briefly describes three of the most common types of depressive disorders. However, within these types there are variat","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129227142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Rathore, Ranjana Punyasi, P. Joshi, Deepika Rathore, D. Bhatnagar
The present study was undertaken to find the protective role of myrobalan against lead (Pb) induced cytogenetic effects on mitosis in Allium-cepa root tip cells. Onions were initially cultivated in deionized water for 3 days and were then exposed at 100, 1000, 3000 and 10,000 ppm of lead nitrate solution for 1 hr. After exposure to lead, the onions were allowed to recover naturally or in myrobalan suspension (0.01 mg/ml) for 72 hrs. The root color, mean root length (MRL) and mitolic index (MI) were recorded and the metaphases and anaphases were scored for chromosomal aberrations. During the natural recovery (NR), roots did not grow following 1000, 3000 and 10,000 ppm Pb exposure but myrobalan treated drug recovery (DR) showed root growth following 1000 and 3000 ppm Pb exposure. The root growth was observed in both NR and DR, more in DR in 100 ppm Pb exposure. Among the control, root growth during these periods and MI did not change throughout the experimentation. Pb exposure at all concentrations, lowered MI. NR was ineffective in Pb treated root tip cells as these were seen in interphase with hypertrophied nucleoli showing mitostatic effect. DR could not revert the mitostatic effect in root tip cells exposed at 10,000 ppm Pb, however, drug could do so in 100, 1000 ppm and 3000 ppm Pb exposure. Both, NR and DR reverted mitostatic effect after 100 ppm Pb exposure and the effect was observed earlier in DR. No chromosomal aberrations could be seen at 1 hr, the cells failed to show typical metaphase arrangement. The effect appeared dose dependent. DR reverted mitostatic effect from 1 hr onwards and completed at 72 hrs in 1000 and 3000 ppm Pb exposure. At 100 ppm Pb exposure, mitostatic effect disappeared at 48 hr in NR while in DR, it reverted after 24 hr. Control root tip cells showed no mitostatic effect.
{"title":"Studies on the reversal of lead induced mitostatic effect in Allium Cepa root tip cells with myrobalan (fruit of Terminalia Chebula, Retz, Combretaceae)","authors":"H. Rathore, Ranjana Punyasi, P. Joshi, Deepika Rathore, D. Bhatnagar","doi":"10.5580/1de8","DOIUrl":"https://doi.org/10.5580/1de8","url":null,"abstract":"The present study was undertaken to find the protective role of myrobalan against lead (Pb) induced cytogenetic effects on mitosis in Allium-cepa root tip cells. Onions were initially cultivated in deionized water for 3 days and were then exposed at 100, 1000, 3000 and 10,000 ppm of lead nitrate solution for 1 hr. After exposure to lead, the onions were allowed to recover naturally or in myrobalan suspension (0.01 mg/ml) for 72 hrs. The root color, mean root length (MRL) and mitolic index (MI) were recorded and the metaphases and anaphases were scored for chromosomal aberrations. During the natural recovery (NR), roots did not grow following 1000, 3000 and 10,000 ppm Pb exposure but myrobalan treated drug recovery (DR) showed root growth following 1000 and 3000 ppm Pb exposure. The root growth was observed in both NR and DR, more in DR in 100 ppm Pb exposure. Among the control, root growth during these periods and MI did not change throughout the experimentation. Pb exposure at all concentrations, lowered MI. NR was ineffective in Pb treated root tip cells as these were seen in interphase with hypertrophied nucleoli showing mitostatic effect. DR could not revert the mitostatic effect in root tip cells exposed at 10,000 ppm Pb, however, drug could do so in 100, 1000 ppm and 3000 ppm Pb exposure. Both, NR and DR reverted mitostatic effect after 100 ppm Pb exposure and the effect was observed earlier in DR. No chromosomal aberrations could be seen at 1 hr, the cells failed to show typical metaphase arrangement. The effect appeared dose dependent. DR reverted mitostatic effect from 1 hr onwards and completed at 72 hrs in 1000 and 3000 ppm Pb exposure. At 100 ppm Pb exposure, mitostatic effect disappeared at 48 hr in NR while in DR, it reverted after 24 hr. Control root tip cells showed no mitostatic effect.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114762747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christina U. Okwuonu, K. A. Oluyemi, Baxter D. Grillo, O. A. Adesanya, V. Ukwenya, Blessing I. Odion, D. Ofusori
25 Cyclic female wistar rats were randomized into 5 groups (A, B, C, D & E) of 5 animals each. They were administered 0.00mg/Kg B.W, 100mg/KgB.W, 150 mg/Kg B.W, 200 mg/Kg B.W, 250 mg/Kg B.W & 300 mg/Kg B.W. of methanolic extract of Aspilia africana respectively, once in a day, everyday of the week for 30 days. The animals' weights were taking before, midway and at the end of the experiment. Reduced wet weights of the ovaries were recorded at the end of experiment. Routine histological analysis of the Ovaries revealed derangement of granulosa cells, degeneration and reduction of follicles and poor vascularity of ovarian stroma.
{"title":"Effects Of Methanolic Extract Of Aspilia Africana Leaf On The Ovarian Tissues And Weights Of Wistar Rats","authors":"Christina U. Okwuonu, K. A. Oluyemi, Baxter D. Grillo, O. A. Adesanya, V. Ukwenya, Blessing I. Odion, D. Ofusori","doi":"10.5580/3f5","DOIUrl":"https://doi.org/10.5580/3f5","url":null,"abstract":"25 Cyclic female wistar rats were randomized into 5 groups (A, B, C, D & E) of 5 animals each. They were administered 0.00mg/Kg B.W, 100mg/KgB.W, 150 mg/Kg B.W, 200 mg/Kg B.W, 250 mg/Kg B.W & 300 mg/Kg B.W. of methanolic extract of Aspilia africana respectively, once in a day, everyday of the week for 30 days. The animals' weights were taking before, midway and at the end of the experiment. Reduced wet weights of the ovaries were recorded at the end of experiment. Routine histological analysis of the Ovaries revealed derangement of granulosa cells, degeneration and reduction of follicles and poor vascularity of ovarian stroma.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128186183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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