Shradha, Vineeta Joshi, S. Maurya, U. P. Singh, G. Nath, Amitabh Singh
Kampillaka (Mallotus philippinensis Muell. Arg.), a promising drug used since 1000 B.C. as anthelmintic, in dermal problems and infectious wounds is commonly found adulterated in the crude drug market. An attempt was made to authenticate the crude drug samples collected from different places of India by subjecting them to HPLC analysis with particular reference to phenolic acids. The sample from Dehradun was found to have high amount of phenolic acids than other samples. Pharmacological activity of the phenolic acids was seen to correspond with the medicinal property of the drug.
{"title":"Authentication of Kampillaka (Mallotus philippinensis): An Important Drug of Ayurveda (Indian Traditional Medicine)","authors":"Shradha, Vineeta Joshi, S. Maurya, U. P. Singh, G. Nath, Amitabh Singh","doi":"10.5580/149b","DOIUrl":"https://doi.org/10.5580/149b","url":null,"abstract":"Kampillaka (Mallotus philippinensis Muell. Arg.), a promising drug used since 1000 B.C. as anthelmintic, in dermal problems and infectious wounds is commonly found adulterated in the crude drug market. An attempt was made to authenticate the crude drug samples collected from different places of India by subjecting them to HPLC analysis with particular reference to phenolic acids. The sample from Dehradun was found to have high amount of phenolic acids than other samples. Pharmacological activity of the phenolic acids was seen to correspond with the medicinal property of the drug.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126505106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Histological studies of the teratogenic effects of oral administration of extract of Aspilia africana, used in ethno medical practice in Africa for the management of various ailments, on the developing kidneys of pregnant matured female Wistar rats were studied. The rats (n=24), average weight of 180g were randomly assigned into two treatment (n=16) and a control (n=8) group. The rats in the treatment groups received 0.5g/kg and 1g/kg of aqueous extract of Aspilia africana orally through orogastric tube in the first seven days of gestation, while the control rats received equal volume of distilled water without the extract of Aspilia added. The rats were fed with growers' mash and were given water liberally. After parturition, the kidney sections were obtained from the pups or neonates and processed for routine histological investigation. Histological changes observed in the kidney sections revealed loss of renal corpuscle and varying degree of cyto-architectural distortion of the cortical structures, with degenerative and atrophic changes. This suggests the direct cytotoxic action of aqueous extract of Aspilia africana resulting from placental transfer during pregnancy to the neonates. This study highlights the possible abnormalities that could result in a newborn when a pregnant animal is exposed to an aqueous extract of Aspilia africana during the seven days of gestation. The results of this experiment suggest that the functions of the kidney could also have been affected as a result of the massive cyto-archictectural distortion. It is recommended that further studies aimed at corroborating these observations be conducted.
{"title":"Histological Studies Of The Teratogenic Effects Of Oral Administration Of Aspilia Africana (Asteraceae) Leaf Extract On The Developing Liver Of Neonatal Wistar Rats","authors":"A. O. Eweka","doi":"10.5580/2025","DOIUrl":"https://doi.org/10.5580/2025","url":null,"abstract":"Histological studies of the teratogenic effects of oral administration of extract of Aspilia africana, used in ethno medical practice in Africa for the management of various ailments, on the developing kidneys of pregnant matured female Wistar rats were studied. The rats (n=24), average weight of 180g were randomly assigned into two treatment (n=16) and a control (n=8) group. The rats in the treatment groups received 0.5g/kg and 1g/kg of aqueous extract of Aspilia africana orally through orogastric tube in the first seven days of gestation, while the control rats received equal volume of distilled water without the extract of Aspilia added. The rats were fed with growers' mash and were given water liberally. After parturition, the kidney sections were obtained from the pups or neonates and processed for routine histological investigation. Histological changes observed in the kidney sections revealed loss of renal corpuscle and varying degree of cyto-architectural distortion of the cortical structures, with degenerative and atrophic changes. This suggests the direct cytotoxic action of aqueous extract of Aspilia africana resulting from placental transfer during pregnancy to the neonates. This study highlights the possible abnormalities that could result in a newborn when a pregnant animal is exposed to an aqueous extract of Aspilia africana during the seven days of gestation. The results of this experiment suggest that the functions of the kidney could also have been affected as a result of the massive cyto-archictectural distortion. It is recommended that further studies aimed at corroborating these observations be conducted.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130898895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Oral candidiasis is presently a common problem affecting children in third world countries. This is probably due to the increasing prevalence of human immunodeficiency virus infections, poverty and malnutrition, which predisposes to candida infections. There are anecdotal reports suggesting the efficacy of the Nigerian grown species of Jatropha multifida herbs in the management of oral candidiasis. Aims of the study: To determine the efficacy of Jatropha multifida in the management of oral candidiasis and compare its efficacy with that of oral Nystatin. Subjects and Method: All the clinically detected cases of children with oral candidiasis at the children's outpatient department of the State Hospital, Osogbo and children's welfare clinic of the Wesley Guild Hospital, Ilesa were randomized into either Jatropha multifadum Juice extract therapy or the Nystatin group. The juice extracts from the Jatropha multifada leaves were applied to the tongue and the oral mucosal areas affected by candida lesions as a single application in the patients randomized to this group. Oral Nystatin was administered 4 times a day, for 7 consecutive days to the children randomized to the Nystatin group. Results: A total of 5 patients (3 boys and 2 girls) were studied with their ages ranging from 2 to 10 months. Clearance of the white lesions on the tongue was defined as cure and this was recorded within 24 hours in the patients on Jatropha multifada juice extracts, while those on oral Nystatin showed features of cure at 48 hours. Conclusion: Jatropha multifada is efficacious in the management of oral candidiasis. Compared to oral Nystatin suspension, it has the advantages of acting faster and being efficacious as a single dose. Its use in the management of oral candidiasis is recommended in third world countries where it is easily cultivated and accessible.
{"title":"The Efficacy Of Jatropha Multifida In The Management Of Oral Candidiasis: A Preliminary Study","authors":"Aladekomo Theophilus Adesola, O. O. Adetunji","doi":"10.5580/23e","DOIUrl":"https://doi.org/10.5580/23e","url":null,"abstract":"Background: Oral candidiasis is presently a common problem affecting children in third world countries. This is probably due to the increasing prevalence of human immunodeficiency virus infections, poverty and malnutrition, which predisposes to candida infections. There are anecdotal reports suggesting the efficacy of the Nigerian grown species of Jatropha multifida herbs in the management of oral candidiasis. Aims of the study: To determine the efficacy of Jatropha multifida in the management of oral candidiasis and compare its efficacy with that of oral Nystatin. Subjects and Method: All the clinically detected cases of children with oral candidiasis at the children's outpatient department of the State Hospital, Osogbo and children's welfare clinic of the Wesley Guild Hospital, Ilesa were randomized into either Jatropha multifadum Juice extract therapy or the Nystatin group. The juice extracts from the Jatropha multifada leaves were applied to the tongue and the oral mucosal areas affected by candida lesions as a single application in the patients randomized to this group. Oral Nystatin was administered 4 times a day, for 7 consecutive days to the children randomized to the Nystatin group. Results: A total of 5 patients (3 boys and 2 girls) were studied with their ages ranging from 2 to 10 months. Clearance of the white lesions on the tongue was defined as cure and this was recorded within 24 hours in the patients on Jatropha multifada juice extracts, while those on oral Nystatin showed features of cure at 48 hours. Conclusion: Jatropha multifada is efficacious in the management of oral candidiasis. Compared to oral Nystatin suspension, it has the advantages of acting faster and being efficacious as a single dose. Its use in the management of oral candidiasis is recommended in third world countries where it is easily cultivated and accessible.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125282587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
QM, 12-year-old girl, was diagnosed with pineocytoma. Surgical resection was not indicated. With time the tumour started to grow in size from 1.5 x 2 x 2.1 cm in 2002 to 2.5 x 2.7 x 3.1 cm in 2004. QM's mother declined radiotherapy and opted for herbal treatment. One year on the herbs, MRI indicated a tumour mass of 2.0 x 2.0 x 3.5 cm. The expected increase of tumour size did not occur. DECLARATION OF INTEREST Retired professor of botany / teacher, respectively, and currently practising herbalists. The senior author has partial financial interest in the use or marketing of the herbs mentioned in this article.
QM, 12岁女孩，被诊断为松果体细胞瘤。不需要手术切除。随着时间的推移，肿瘤的大小开始从2002年的1.5 x 2 x 2.1厘米增长到2004年的2.5 x 2.7 x 3.1厘米。QM的母亲拒绝了放疗，选择了草药治疗。服用草药一年，MRI显示肿瘤肿块为2.0 x 2.0 x 3.5 cm。预期的肿瘤增大并没有发生。退休植物学教授/教师，现为执业草药医师。资深作者在本文中提到的草药的使用或营销中有部分经济利益。
{"title":"Arresting the Progressive Growth of Pineocytoma with Herbal Therapy","authors":"C. Teo, Ch'ng Beng Im-Teo","doi":"10.5580/ef5","DOIUrl":"https://doi.org/10.5580/ef5","url":null,"abstract":"QM, 12-year-old girl, was diagnosed with pineocytoma. Surgical resection was not indicated. With time the tumour started to grow in size from 1.5 x 2 x 2.1 cm in 2002 to 2.5 x 2.7 x 3.1 cm in 2004. QM's mother declined radiotherapy and opted for herbal treatment. One year on the herbs, MRI indicated a tumour mass of 2.0 x 2.0 x 3.5 cm. The expected increase of tumour size did not occur. DECLARATION OF INTEREST Retired professor of botany / teacher, respectively, and currently practising herbalists. The senior author has partial financial interest in the use or marketing of the herbs mentioned in this article.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125571142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To show that the Ugu vegetable extracts (Telfaira Occidentals) is efficacious in the management of severe anemia in the paediatric age groups. Methods: Case reports of two severely anemic patients, whose parents refused blood transfusion and were subsequently managed with oral intakes of the Ugu vegetable extracts, with satisfactory rise in the hematocrit levels. Results: The preUgu administration pack cell volume of 15% in both of them, rose to 20% in one and 25% in the other, 24 hours post administration of the extract. Conclusion: The Ugu vegetable extract was efficacious in the management of severe anemia in these 2 children and may be useful in pediatric patients with severe anemia whose parents refuse blood transfusion. The vegetable extract may have an even greater role in the prevention of anemia if intake is instituted early. Wider studies are needed to investigate these hypotheses.
{"title":"The Efficacy Of Ugu (Telfaira Occidentals) In The Management of Childhood Anemia: A Report of 2 Cases","authors":"O. Oyedeji","doi":"10.5580/2551","DOIUrl":"https://doi.org/10.5580/2551","url":null,"abstract":"Purpose: To show that the Ugu vegetable extracts (Telfaira Occidentals) is efficacious in the management of severe anemia in the paediatric age groups. Methods: Case reports of two severely anemic patients, whose parents refused blood transfusion and were subsequently managed with oral intakes of the Ugu vegetable extracts, with satisfactory rise in the hematocrit levels. Results: The preUgu administration pack cell volume of 15% in both of them, rose to 20% in one and 25% in the other, 24 hours post administration of the extract. Conclusion: The Ugu vegetable extract was efficacious in the management of severe anemia in these 2 children and may be useful in pediatric patients with severe anemia whose parents refuse blood transfusion. The vegetable extract may have an even greater role in the prevention of anemia if intake is instituted early. Wider studies are needed to investigate these hypotheses.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133958658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants are sources of medication from the ancient times. Bidents tripartitata is a plant, which grows in Anatolia. It is believed that it relieves pain of arthritis, with per oral administration of its extract. This article presents 2 cases of massive skin necrosis due to topical application of bidents tripartitata. One of the patients had applied it topically on his knees and had developed severe necrosis. The other patient had contact with this plant unintentionally. She had skin necrosis on the dorsum of her hand and forearm. Bidents tripartitata is believed to have sedative, diuretic and analgesic effects. The plant includes ether, flavonoids, ascorbic acid, carotene and manganese. Today there is an increasing trend to use natural drugs in treatment of many diseases. It does not mean that all the plants are useful, sometimes the results may be very harmful.
{"title":"Massive Skin Necrosis Due To Pitirak (Bidents Tripartitata)","authors":"A. Terzioğlu, N. T. Başer, L. Levent, G. Aslan","doi":"10.5580/1cef","DOIUrl":"https://doi.org/10.5580/1cef","url":null,"abstract":"Plants are sources of medication from the ancient times. Bidents tripartitata is a plant, which grows in Anatolia. It is believed that it relieves pain of arthritis, with per oral administration of its extract. This article presents 2 cases of massive skin necrosis due to topical application of bidents tripartitata. One of the patients had applied it topically on his knees and had developed severe necrosis. The other patient had contact with this plant unintentionally. She had skin necrosis on the dorsum of her hand and forearm. Bidents tripartitata is believed to have sedative, diuretic and analgesic effects. The plant includes ether, flavonoids, ascorbic acid, carotene and manganese. Today there is an increasing trend to use natural drugs in treatment of many diseases. It does not mean that all the plants are useful, sometimes the results may be very harmful.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132614368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This article covers introductory concepts of pulmonary sarcoidosis from both a Western medical perspective and a Traditional Chinese Medical (TCM) perspective. An overview is given on the Western pathology of pulmonary sarcoidosis. Energetic functions of the Lungs in TCM are described, followed by the energetic etiology. Possible treatment strategies are enumerated for the main energetic types of pulmonary sarcoidosis. Also included are tables and figures to provide a visual context.
{"title":"Pulmonary Sarcoidosis and Traditional Chinese Medicine","authors":"Timothy L. Hardesty","doi":"10.5580/fdb","DOIUrl":"https://doi.org/10.5580/fdb","url":null,"abstract":"This article covers introductory concepts of pulmonary sarcoidosis from both a Western medical perspective and a Traditional Chinese Medical (TCM) perspective. An overview is given on the Western pathology of pulmonary sarcoidosis. Energetic functions of the Lungs in TCM are described, followed by the energetic etiology. Possible treatment strategies are enumerated for the main energetic types of pulmonary sarcoidosis. Also included are tables and figures to provide a visual context.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116671235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: Effective and objective method for decide the location of primary headache is needed. In this study we used corresponding points on the middle finger of hand of Korean Hand Therapy (KHT). Subjects and Methods: The 600 outpatients with primary headache were included. History, physical exam and routine laboratory tests were performed. On physical examination, we palpated head and middle finger together and compared with each other. We decided the right or left side and sites of one side. The palpated points were bilateral forehead, periorbital, preauricular, temporal, parietal, and occipital, 2-3 centimeter apart from midline of head and neck from forehead to occipital. On the middle finger we pressed KHT points such as Urinary Bladder micromeridian(I) I-1, I-2, I-3 and Gallbladder micromeridian(M) M-1, M-2, M-3, M-4, M-5, and M-6 using tip probe and or roller stimulator. Results: Among 400 migraineurs, 48 had pain sensitive points on the both sides of head and middle fingers, 196 had on the right side and 156 had on the left side. The painful sites were diffuse rather than localized. Among 200 patients with tension type headache, 67 had sensitive points on the right side along midline of head and middle finger, 105 had sensitive points on the left side along midline and 28 had sensitive points on both side along midline. Conclusion: For diagnosis and treatment of primary headache objectively and effectively, we can save the time to decide and to confirm location during physical examination if we apply corresponding points of KHT.
{"title":"Useful Method To Confirm Tender Points Of Primary Headache: Corresponding Points Of Koryo Hand Acupuncture Therapy","authors":"K. Park, T. Yoo","doi":"10.5580/dac","DOIUrl":"https://doi.org/10.5580/dac","url":null,"abstract":"Purpose: Effective and objective method for decide the location of primary headache is needed. In this study we used corresponding points on the middle finger of hand of Korean Hand Therapy (KHT). Subjects and Methods: The 600 outpatients with primary headache were included. History, physical exam and routine laboratory tests were performed. On physical examination, we palpated head and middle finger together and compared with each other. We decided the right or left side and sites of one side. The palpated points were bilateral forehead, periorbital, preauricular, temporal, parietal, and occipital, 2-3 centimeter apart from midline of head and neck from forehead to occipital. On the middle finger we pressed KHT points such as Urinary Bladder micromeridian(I) I-1, I-2, I-3 and Gallbladder micromeridian(M) M-1, M-2, M-3, M-4, M-5, and M-6 using tip probe and or roller stimulator. Results: Among 400 migraineurs, 48 had pain sensitive points on the both sides of head and middle fingers, 196 had on the right side and 156 had on the left side. The painful sites were diffuse rather than localized. Among 200 patients with tension type headache, 67 had sensitive points on the right side along midline of head and middle finger, 105 had sensitive points on the left side along midline and 28 had sensitive points on both side along midline. Conclusion: For diagnosis and treatment of primary headache objectively and effectively, we can save the time to decide and to confirm location during physical examination if we apply corresponding points of KHT.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130342163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An 82-year-old female was diagnosed with colon cancer. A large mass was found obstructing the colonic passage at the hepatic flexure. The family declined surgery and opted for herbal treatment. Within two weeks, the patient's condition was restored to normacy.
{"title":"Cancerous Colonic Obstruction at Hepatic Flexure Relieved by Herbs: A Case Study","authors":"C. Teo, Ch'ng Beng Im-Teo","doi":"10.5580/1f5f","DOIUrl":"https://doi.org/10.5580/1f5f","url":null,"abstract":"An 82-year-old female was diagnosed with colon cancer. A large mass was found obstructing the colonic passage at the hepatic flexure. The family declined surgery and opted for herbal treatment. Within two weeks, the patient's condition was restored to normacy.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115011689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim of the study: We aimed to measure lipid peroxidation and antioxidant enzymes in erythrocytes before and after ischemiareperfusion in a rat hindlimb model, and to compare the effects of reperfusion with Vitamin C and dexamethasone on the erythrocyte antioxidant capacity. Material and method: Thiobarbituric acid -reactive substances (TBARS), a measure of lipid peroxidation, Superoxide dismutase (SOD), Glutathion (GSH) and catalase (CAT) were determined in erythrocytes. Erythrocytes were prepared by classical washing method (0,9 %Nacl) from venous samples. A total of 42 rats were used in the study which were divided into 6 groups 7 rats in each as follows: control group or the sham-operated group, short ischemia-reperfusion, long ischemiareperfusion, short ischemia and reperfusion with ascorbic acid (AA), short ischemia and reperfusion with dexamethasone, the double extremity I-R with short duration groups. A non-traumatic vascular clamp (Acland, V3 vascular clamp) was placed accross the right iliac artery for 45 minutes in short ischemia and for 3 hours in long ischemia groups. Venous blood samples were taken to evaluate the Cat, SOD, GSH and TBARS levels in erythrocytes at the completion of reperfusion. Results: Elevated TBARS levels and decreased Cat, SOD and GSH levels in erythrocytes were seen after i-r of rat hindlimb (P<0.001). When compared with reperfusion with dexamethasone, AA had a more improved effect on antioxidant capacity of erythrocytes after ischemia of hindlimb in rats. Conclusions: Reperfusion with AA after ischemia resulted an improved antioxidant capacity preservation in erythrocytes. In clinical setting, addition of vitamin C to reperfusion solution may be benefical.
{"title":"Improved Preservation Of Erythrocyte Antioxidant Capacity With Ascorbic Acid Reperfusion After Ischemia: A Comparative Study In A Rat Hindlimb Model","authors":"Y. Coban, E. Bulbuloglu, A. Polat, F. Inanç","doi":"10.5580/c56","DOIUrl":"https://doi.org/10.5580/c56","url":null,"abstract":"Aim of the study: We aimed to measure lipid peroxidation and antioxidant enzymes in erythrocytes before and after ischemiareperfusion in a rat hindlimb model, and to compare the effects of reperfusion with Vitamin C and dexamethasone on the erythrocyte antioxidant capacity. Material and method: Thiobarbituric acid -reactive substances (TBARS), a measure of lipid peroxidation, Superoxide dismutase (SOD), Glutathion (GSH) and catalase (CAT) were determined in erythrocytes. Erythrocytes were prepared by classical washing method (0,9 %Nacl) from venous samples. A total of 42 rats were used in the study which were divided into 6 groups 7 rats in each as follows: control group or the sham-operated group, short ischemia-reperfusion, long ischemiareperfusion, short ischemia and reperfusion with ascorbic acid (AA), short ischemia and reperfusion with dexamethasone, the double extremity I-R with short duration groups. A non-traumatic vascular clamp (Acland, V3 vascular clamp) was placed accross the right iliac artery for 45 minutes in short ischemia and for 3 hours in long ischemia groups. Venous blood samples were taken to evaluate the Cat, SOD, GSH and TBARS levels in erythrocytes at the completion of reperfusion. Results: Elevated TBARS levels and decreased Cat, SOD and GSH levels in erythrocytes were seen after i-r of rat hindlimb (P<0.001). When compared with reperfusion with dexamethasone, AA had a more improved effect on antioxidant capacity of erythrocytes after ischemia of hindlimb in rats. Conclusions: Reperfusion with AA after ischemia resulted an improved antioxidant capacity preservation in erythrocytes. In clinical setting, addition of vitamin C to reperfusion solution may be benefical.","PeriodicalId":107168,"journal":{"name":"The Internet Journal of Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2004-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125802861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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