Purpose: Novel coronavirus disease 2019 (COVID-19) is an infectious disease unknown before the 2019 outbreak in Wuhan. This study evaluated telomere length in COVID-19 (+) and (-) samples with clinical-demographic parameters.
Materials and Methods: DNA was isolated from COVID-19 (+) (n=70) and (-) (n=70) patients. Telomere length was determined by real-time-PCR (RT-PCR). The 2–∆∆Ct method was used to analyze the telomere length of the samples.
Results: There were significant differences in creatinine, LDH, ferritin, WBC, NEU and CRP in COVID-19 (+) patients compared to COVID-19 (-) patients. The NEU/LYM (or N/L) ratio was found higher in the patients with COVID-19 (+), than in COVID-19 (-). On the other hand, our COVID-19 (+) patients (mean±std:0.93±0.58) had significantly shorter telomere lengths than the COVID-19 (-) (mean±std:1.26±0.76). Moreover, COVID-19 (+) male patients (mean±std:1.06±0.50) had longer telomere length than female patients (mean±std:0.76±0.54). Telomere length was significantly shorter in patients with COVID-19 (+)with high blood urea nitrogen (BUN), high creatinine, high hematocrit, high NEU levels, normal platelets (PLT), and low WBC levels.
Conclusions: Our findings suggest that telomere length and blood parameter levels influence the severity of COVID-19. Blood parameters differed in patients with COVID-19 (+) and COVID-19 (-). As a result, increasing the number of similar studies in the future can demonstrate the significance of our findings.
Keywords: COVID-19, Telomere length, RT-PCR, NEU/LYM, blood
{"title":"Analysis of telomere length in patients with COVID-19 and investigation into its relationship with clinical- demographic data","authors":"Atakan SAVRUN, Ebubekir DİRİCAN","doi":"10.17826/cumj.1312436","DOIUrl":"https://doi.org/10.17826/cumj.1312436","url":null,"abstract":"Purpose: Novel coronavirus disease 2019 (COVID-19) is an infectious disease unknown before the 2019 outbreak in Wuhan. This study evaluated telomere length in COVID-19 (+) and (-) samples with clinical-demographic parameters. 
 Materials and Methods: DNA was isolated from COVID-19 (+) (n=70) and (-) (n=70) patients. Telomere length was determined by real-time-PCR (RT-PCR). The 2–∆∆Ct method was used to analyze the telomere length of the samples.
 Results: There were significant differences in creatinine, LDH, ferritin, WBC, NEU and CRP in COVID-19 (+) patients compared to COVID-19 (-) patients. The NEU/LYM (or N/L) ratio was found higher in the patients with COVID-19 (+), than in COVID-19 (-). On the other hand, our COVID-19 (+) patients (mean±std:0.93±0.58) had significantly shorter telomere lengths than the COVID-19 (-) (mean±std:1.26±0.76). Moreover, COVID-19 (+) male patients (mean±std:1.06±0.50) had longer telomere length than female patients (mean±std:0.76±0.54). Telomere length was significantly shorter in patients with COVID-19 (+)with high blood urea nitrogen (BUN), high creatinine, high hematocrit, high NEU levels, normal platelets (PLT), and low WBC levels. 
 Conclusions: Our findings suggest that telomere length and blood parameter levels influence the severity of COVID-19. Blood parameters differed in patients with COVID-19 (+) and COVID-19 (-). As a result, increasing the number of similar studies in the future can demonstrate the significance of our findings. 
 Keywords: COVID-19, Telomere length, RT-PCR, NEU/LYM, blood","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: In the acute phase of Cerebral Venous Sinus Thrombosis (CVST), approximately 35-50 % of patients diagnosed epileptic seizures. This study aimed to investigate the demographic and clinical characteristics, frequency of seizures, prognosis of patients during a one-year follow-up period.
Materials and Methods: The files of patients who were followed up with the diagnosis of acute ischemic stroke between January 1, 2018 and January 1, 2022 were evaluated in this retrospective study. Demographic features, presence of seizure history and seizure frequency, neuroimaging findings, affected cerebral venous sinuses, and anti-seizure medication use were recorded.
Results: A total of 701 patients with acute stroke had their medical records reviewed. Thirty-two of 701 patients (4.56 %) were diagnosed with CVST; 37.5 % of patients experienced early-onset seizures. Seizure control was achieved with monotherapy in 88.8 % (n:8) of patients with seizures. Involved sinuses in patients with seizures; 50 % (n=6) superior sagittal sinus was the most commonly involved sinus and inferior sagittal sinus and sinus rectus were not involved. In terms of sinus involvement, there was no statistically significant difference between patients with and without seizures. On cerebral magnetic resonance imaging, venous infarcts were detected in 66.7 % (n=8) of patients with seizures, compared to 35 % of patients without seizures.
Conclusion: This retrospective study revealed that seizures typically occur early in the disease, have a favorable prognosis over the long term, and can be managed with monotherapy.
{"title":"Sinus vein thrombosis and epileptic seizures: outcomes of long term follow-up","authors":"Mehmet Taylan PEKÖZ, Turgay DEMİR","doi":"10.17826/cumj.1326857","DOIUrl":"https://doi.org/10.17826/cumj.1326857","url":null,"abstract":"Purpose: In the acute phase of Cerebral Venous Sinus Thrombosis (CVST), approximately 35-50 % of patients diagnosed epileptic seizures. This study aimed to investigate the demographic and clinical characteristics, frequency of seizures, prognosis of patients during a one-year follow-up period.
 Materials and Methods: The files of patients who were followed up with the diagnosis of acute ischemic stroke between January 1, 2018 and January 1, 2022 were evaluated in this retrospective study. Demographic features, presence of seizure history and seizure frequency, neuroimaging findings, affected cerebral venous sinuses, and anti-seizure medication use were recorded.
 Results: A total of 701 patients with acute stroke had their medical records reviewed. Thirty-two of 701 patients (4.56 %) were diagnosed with CVST; 37.5 % of patients experienced early-onset seizures. Seizure control was achieved with monotherapy in 88.8 % (n:8) of patients with seizures. Involved sinuses in patients with seizures; 50 % (n=6) superior sagittal sinus was the most commonly involved sinus and inferior sagittal sinus and sinus rectus were not involved. In terms of sinus involvement, there was no statistically significant difference between patients with and without seizures. On cerebral magnetic resonance imaging, venous infarcts were detected in 66.7 % (n=8) of patients with seizures, compared to 35 % of patients without seizures.
 Conclusion: This retrospective study revealed that seizures typically occur early in the disease, have a favorable prognosis over the long term, and can be managed with monotherapy.","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Green tea is a widely consumed beverage renowned for its numerous positive health effects. However, it is crucial to be cognizant of certain considerations, as excessive consumption can result in adverse outcomes. Green tea offers numerous health benefits; however, certain considerations should be taken into account. Therefore, it is essential for individuals to tailor their green tea consumption based on their specific health status and requirements.
{"title":"Yeşil çay içerken neden dikkatli olmalıyız?","authors":"İzzet FİDANCI","doi":"10.17826/cumj.1311590","DOIUrl":"https://doi.org/10.17826/cumj.1311590","url":null,"abstract":"Green tea is a widely consumed beverage renowned for its numerous positive health effects. However, it is crucial to be cognizant of certain considerations, as excessive consumption can result in adverse outcomes. Green tea offers numerous health benefits; however, certain considerations should be taken into account. Therefore, it is essential for individuals to tailor their green tea consumption based on their specific health status and requirements.","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Özge ZORLU, Sevil KARABAĞ, Kivilcim ERDOGAN, Evin KUŞSEVER, İlke ÖZER ASLAN
Purpose: In animals, the appropriate levels of Enhancer of zeste homolog 2 (EZH2), a well-established epigenetic regulator, are essential for the embryonic development of skin and appendages. We aimed to explore the EZH2 expression patterns in the skin of human fetuses and compare them with adults.
Materials and Methods: We performed EZH2 immunohistochemical staining in skin samples from the scalp region of 67 fetuses and from the farthest surgical margin of the lip wedge resection, where no lesions were found, of 23 adults. EZH2 scores were evaluated according to the literature.
Results: Epidermal (r = − 0.528), dermal (rs = − 0.509), and hair follicle (r = − 0.576) EZH2 scores were inversely correlated with gestational age. Epidermal and hair follicle EZH2 scores were significantly higher in adults compared to fetuses. There were significant and positive correlations between epidermal, dermal, and hair follicle EZH2 scores among fetuses and adults.
Conclusion: Lower levels of EZH2 may be necessary for final cutaneous differentiation and maturation before birth. Our findings may have a therapeutic impact on cutaneous disorders with differentiation defects, chronic wounds, and alopecias.
{"title":"Gelişmekte olan insan fetüslerinin ve yetişkinlerin derisinde EZH2 ekspresyonu: karşılaştırmalı bir çalışma","authors":"Özge ZORLU, Sevil KARABAĞ, Kivilcim ERDOGAN, Evin KUŞSEVER, İlke ÖZER ASLAN","doi":"10.17826/cumj.1321745","DOIUrl":"https://doi.org/10.17826/cumj.1321745","url":null,"abstract":"Purpose: In animals, the appropriate levels of Enhancer of zeste homolog 2 (EZH2), a well-established epigenetic regulator, are essential for the embryonic development of skin and appendages. We aimed to explore the EZH2 expression patterns in the skin of human fetuses and compare them with adults.
 Materials and Methods: We performed EZH2 immunohistochemical staining in skin samples from the scalp region of 67 fetuses and from the farthest surgical margin of the lip wedge resection, where no lesions were found, of 23 adults. EZH2 scores were evaluated according to the literature. 
 Results: Epidermal (r = − 0.528), dermal (rs = − 0.509), and hair follicle (r = − 0.576) EZH2 scores were inversely correlated with gestational age. Epidermal and hair follicle EZH2 scores were significantly higher in adults compared to fetuses. There were significant and positive correlations between epidermal, dermal, and hair follicle EZH2 scores among fetuses and adults.
 Conclusion: Lower levels of EZH2 may be necessary for final cutaneous differentiation and maturation before birth. Our findings may have a therapeutic impact on cutaneous disorders with differentiation defects, chronic wounds, and alopecias.","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: The aim of this study was to investigate the structural and electrical changes in the heart associated with exercise in young athletes under 18 years of age and to examine the relationship between these changes and biochemical markers.
Materials and Methods: In this study, 32 male paediatric athletes (five rowers, five kayakers and 22 footballers) were prospectively evaluated. Dynamic exercise training for 75 minutes was applied to all athletes. An echocardiographic evaluation was performed on the athletes at rest. An electrocardiogram (ECG) was taken before and after the exercise. Blood samples were taken from all athletes before the exercise, during the first hour of exercise and after the fourth hour of the training session to analyse the serum levels of creatine kinase myocardial band, troponin T(cTnT) and pro-brain natriuretic peptide.
Results: The mean age of all athletes was 15.3±0.9 years. In the echocardiography examination, end-diastolic thickness, left ventricular end-diastolic diameter, posterior wall end-diastolic diameter, left ventricular end-systolic diameter, left ventricular mass measurements (LVM) and left ventricular mass indices (LVMI) were higher among the rowers than in the remaining groups. In the post-exercise ECG, T-wave peaks were observed in 16 individuals, including all rowers, all kayakers, and six footballers. The cTnT values in the first hour and after the fourth were 0.31–0.71 ng/ml in football players, 0.10–0.27 ng/ml in rowers, and 0.04–0.15 ng/ml kayakers. An increase in cTnT levels was detected after the fourth hour of exercise in all groups. There was a correlation between T-wave peaks in the ECG taken after exercise and age, cTnT serum level and increased LVM.
Conclusion: In this study, we found T-wave peaks in half of the athletes after exercise. The serum cTnT levels of young athletes may be elevated by exercise and that LVM and LVMI may be higher in athletes. Echocardiography and ECG evaluations should be performed in paediatric athletes.
{"title":"Evaluation of cardiac biomarkers, electrocardiogram changes and echocardiography findings before and after exercise in paediatric athletes","authors":"Semine ÖZDEMİR DİLEK, Sevcan ERDEM, Sanlı Sadi K:URDAK, Nazan ÖZBARLAS","doi":"10.17826/cumj.1325501","DOIUrl":"https://doi.org/10.17826/cumj.1325501","url":null,"abstract":"Purpose: The aim of this study was to investigate the structural and electrical changes in the heart associated with exercise in young athletes under 18 years of age and to examine the relationship between these changes and biochemical markers.
 Materials and Methods: In this study, 32 male paediatric athletes (five rowers, five kayakers and 22 footballers) were prospectively evaluated. Dynamic exercise training for 75 minutes was applied to all athletes. An echocardiographic evaluation was performed on the athletes at rest. An electrocardiogram (ECG) was taken before and after the exercise. Blood samples were taken from all athletes before the exercise, during the first hour of exercise and after the fourth hour of the training session to analyse the serum levels of creatine kinase myocardial band, troponin T(cTnT) and pro-brain natriuretic peptide.
 Results: The mean age of all athletes was 15.3±0.9 years. In the echocardiography examination, end-diastolic thickness, left ventricular end-diastolic diameter, posterior wall end-diastolic diameter, left ventricular end-systolic diameter, left ventricular mass measurements (LVM) and left ventricular mass indices (LVMI) were higher among the rowers than in the remaining groups. In the post-exercise ECG, T-wave peaks were observed in 16 individuals, including all rowers, all kayakers, and six footballers. The cTnT values in the first hour and after the fourth were 0.31–0.71 ng/ml in football players, 0.10–0.27 ng/ml in rowers, and 0.04–0.15 ng/ml kayakers. An increase in cTnT levels was detected after the fourth hour of exercise in all groups. There was a correlation between T-wave peaks in the ECG taken after exercise and age, cTnT serum level and increased LVM. 
 Conclusion: In this study, we found T-wave peaks in half of the athletes after exercise. The serum cTnT levels of young athletes may be elevated by exercise and that LVM and LVMI may be higher in athletes. Echocardiography and ECG evaluations should be performed in paediatric athletes.","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: The genus Trichosporon, which is a yeast-like basidiomycete, is ubiquitous in nature and a part of human microbiota. It’s an oppurtunistic fungal pathogen, which was once rare, but increasing dramatically recently, leading to fatal infections. The aim of this study was to determine the prevalence, and antifungal susceptibility of clinical Trichosporon spp. isolates, and to determine whether there was a change in incidence during the COVID-19 Pandemic.
Materials and Methods: This was a retrospective cross-sectional descriptive study, conducted between January 1, 2013 and February 5, 2023. Cultures which Trichosporon spp. isolated (n=125) were screened, and those met the eligibility criteria were included (n=77). Identification, antifungal susceptibility test results, age, gender records were identified from Microbiology Laboratory Information Management System.
Results: 97.4% of the isolates were Trichosporon asahii, and 2.6% were Trichosporon mucoides. The most frequent isolation period was the 2016 and 2019 year group (71.4%), not during the COVID-19 Pandemics (19.5%). The most common sample type was urine (58.4%), of whom predominantly (58.4%) hospitalized in the Intensive Care Unit. Amphotericin B MICs were ≤1µg/ml in 68.9% of the isolates. Fluconazole and voriconazole MICs were ≤1µg/ml in 20.0% and 94.8%, respectively. 59.7% of the isolates had a MIC of ≥4µg/ml for flucytosine. Micafungin and caspofungin MICs were ≥4µg/ml in 88.3% and 92.2% of the isolates, respectively. Voriconazole had the strongest in vitro activity, and amphotericin B had lower MICs than expected. The combination therapy of voriconazole and amphotericin B could be a therapeutic option in this setting, as well as monotherapy of voriconazole.
Conclusion: Trichosporon spp. isolated in clinical specimens and their antifungal susceptibility depend on the geographic region and the anatomic site. Identifying local data will contribute to both the management of these patients, and surveillance studies.
{"title":"Distribution and antifungal susceptibility of clinical Trichosporon spp. isolates: 10 years of single-center experience","authors":"Hatice Hale GÜMÜŞ","doi":"10.17826/cumj.1348790","DOIUrl":"https://doi.org/10.17826/cumj.1348790","url":null,"abstract":"Purpose: The genus Trichosporon, which is a yeast-like basidiomycete, is ubiquitous in nature and a part of human microbiota. It’s an oppurtunistic fungal pathogen, which was once rare, but increasing dramatically recently, leading to fatal infections. The aim of this study was to determine the prevalence, and antifungal susceptibility of clinical Trichosporon spp. isolates, and to determine whether there was a change in incidence during the COVID-19 Pandemic. 
 Materials and Methods: This was a retrospective cross-sectional descriptive study, conducted between January 1, 2013 and February 5, 2023. Cultures which Trichosporon spp. isolated (n=125) were screened, and those met the eligibility criteria were included (n=77). Identification, antifungal susceptibility test results, age, gender records were identified from Microbiology Laboratory Information Management System. 
 Results: 97.4% of the isolates were Trichosporon asahii, and 2.6% were Trichosporon mucoides. The most frequent isolation period was the 2016 and 2019 year group (71.4%), not during the COVID-19 Pandemics (19.5%). The most common sample type was urine (58.4%), of whom predominantly (58.4%) hospitalized in the Intensive Care Unit. Amphotericin B MICs were ≤1µg/ml in 68.9% of the isolates. Fluconazole and voriconazole MICs were ≤1µg/ml in 20.0% and 94.8%, respectively. 59.7% of the isolates had a MIC of ≥4µg/ml for flucytosine. Micafungin and caspofungin MICs were ≥4µg/ml in 88.3% and 92.2% of the isolates, respectively. Voriconazole had the strongest in vitro activity, and amphotericin B had lower MICs than expected. The combination therapy of voriconazole and amphotericin B could be a therapeutic option in this setting, as well as monotherapy of voriconazole.
 Conclusion: Trichosporon spp. isolated in clinical specimens and their antifungal susceptibility depend on the geographic region and the anatomic site. Identifying local data will contribute to both the management of these patients, and surveillance studies.","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136278782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: Traumatic brain injury (TBI) is a serious illness that causes behavioral disorders such as locomotor activity, learning, and memory. This study aims to investigate the behavioral effects of transcranial Direct Current Stimulation (tDCS) treatment on locomotor activity in an experimental traumatic brain injury model and to investigate the levels of IL-1β and IL-18 in the motor cortex tissue.
Materials and Methods: 30 male 3-month-old Wistar albino rats were used. The TBI model was established using the Marmarou method. 2 hours after TBI, sham and TBI+tDCS groups were treated with 0.5 mA 30 minutes anodal tDCS treatment for 2 days. Locomotor activity was evaluated in open field test. IL-1β and IL-18 levels in motor cortex tissue were measured by the ELISA method..
Results: Compared to the sham group, locomotor activity results showed significant decreases in the TBI group while the TBI+tDCS group showed significant increases compared to the TBI group. There were significant increases in IL-1β and IL-18 values in the motor cortex of the animals in the TBI group compared to the sham group, while there was a significant decrease in the TBI+tDCS group compared to the TBI group.
Conclusion: tDCS treatment was shown to have therapeutic effects on neuroinflammation against traumatic brain injury.
目的:创伤性脑损伤(TBI)是一种严重的疾病，可导致运动活动、学习和记忆等行为障碍。本研究旨在探讨经颅直流电刺激(tDCS)治疗对实验性颅脑损伤模型运动活动的行为影响，并探讨运动皮层组织中IL-1β和IL-18的水平。
材料与方法:选用雄性3月龄Wistar白化大鼠30只。采用Marmarou方法建立TBI模型。TBI后2小时，假手术组和TBI+tDCS组均给予0.5 mA 30分钟的负极tDCS治疗，持续2天。在野外试验中评估运动活动。ELISA法检测大鼠运动皮质组织中IL-1β、IL-18水平。& # x0D;结果:与假手术组相比，TBI组的运动活动结果明显降低，而TBI+tDCS组的运动活动结果明显高于TBI组。与假手术组相比，TBI组大鼠运动皮质IL-1β和IL-18值显著升高，而TBI+tDCS组与TBI组相比，IL-1β和IL-18值显著降低。
结论:tDCS治疗对外伤性脑损伤的神经炎症有一定的治疗作用。
{"title":"Therapeutic effects of transcranial direct current stimulation on loss of motor function caused by experimental mild traumatic brain injury","authors":"Güven AKÇAY, Recep BAYDEMİR","doi":"10.17826/cumj.1337529","DOIUrl":"https://doi.org/10.17826/cumj.1337529","url":null,"abstract":"Purpose: Traumatic brain injury (TBI) is a serious illness that causes behavioral disorders such as locomotor activity, learning, and memory. This study aims to investigate the behavioral effects of transcranial Direct Current Stimulation (tDCS) treatment on locomotor activity in an experimental traumatic brain injury model and to investigate the levels of IL-1β and IL-18 in the motor cortex tissue.
 Materials and Methods: 30 male 3-month-old Wistar albino rats were used. The TBI model was established using the Marmarou method. 2 hours after TBI, sham and TBI+tDCS groups were treated with 0.5 mA 30 minutes anodal tDCS treatment for 2 days. Locomotor activity was evaluated in open field test. IL-1β and IL-18 levels in motor cortex tissue were measured by the ELISA method.. 
 Results: Compared to the sham group, locomotor activity results showed significant decreases in the TBI group while the TBI+tDCS group showed significant increases compared to the TBI group. There were significant increases in IL-1β and IL-18 values in the motor cortex of the animals in the TBI group compared to the sham group, while there was a significant decrease in the TBI+tDCS group compared to the TBI group.
 Conclusion: tDCS treatment was shown to have therapeutic effects on neuroinflammation against traumatic brain injury.","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136276531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: The aim of this study was is to compare brain structure volume, including cortical and subcortical regions of adolescents- methamphetamine users versus non-users.
Materials and Methods: The study was designed to be cross-sectional, and structural magnetic resonance imaging scans were obtained from the participants, including ten methamphetamine users and nine non-users. volBrain program was used to evaluate the images.
Results: The results showed that methamphetamine users altered brain structures- temporal, parietal lobes, nucleus accumbens, amygdala, hippocampus, and thalamus volume. Also, the statistically significant difference in the volume between methamphetamine users and non-users was found in subcortical regions except putamen by age. Volumetric analysis of methamphetamine use in adolescents confirms a reduction in temporal lobes (methamphetamine users M±SD=3.43±0.18 non-users M±SD=3.48±0.22) and parietal lobes (methamphetamine users M±SD=2.23±0.24, non-users M±SD=2.37±0.33) in cortical regions in the brain as tissue volume. However, methamphetamine uses caused an increase in volume in the subcortical regions.
Conclusion: Methamphetamine use appears to show decreased volume in the brain regions with age, which has adverse effects on cognitive, emotional, memory, and social abilities.
{"title":"A neuroimaging study of altered cortical and subcortical volume in adolescent methamphetamine users","authors":"Zekeriya TEMİRCAN","doi":"10.17826/cumj.1349328","DOIUrl":"https://doi.org/10.17826/cumj.1349328","url":null,"abstract":"Purpose: The aim of this study was is to compare brain structure volume, including cortical and subcortical regions of adolescents- methamphetamine users versus non-users.
 Materials and Methods: The study was designed to be cross-sectional, and structural magnetic resonance imaging scans were obtained from the participants, including ten methamphetamine users and nine non-users. volBrain program was used to evaluate the images. 
 Results: The results showed that methamphetamine users altered brain structures- temporal, parietal lobes, nucleus accumbens, amygdala, hippocampus, and thalamus volume. Also, the statistically significant difference in the volume between methamphetamine users and non-users was found in subcortical regions except putamen by age. Volumetric analysis of methamphetamine use in adolescents confirms a reduction in temporal lobes (methamphetamine users M±SD=3.43±0.18 non-users M±SD=3.48±0.22) and parietal lobes (methamphetamine users M±SD=2.23±0.24, non-users M±SD=2.37±0.33) in cortical regions in the brain as tissue volume. However, methamphetamine uses caused an increase in volume in the subcortical regions.
 Conclusion: Methamphetamine use appears to show decreased volume in the brain regions with age, which has adverse effects on cognitive, emotional, memory, and social abilities.","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dilan ÇETİNAVCI, Engin YENİLMEZ, Ayşe Firuze BIYIK, Ahmet ALVER, Neslihan SAĞLAM
Purpose: Cyclophosphamide (CP) is an antineoplastic agent. It is used in the treatment of many types of cancer. Rosmarinic acid (RA) exhibits remarkable biological activities such as anti-inflammatory, antitumor, antibacterial, and antimicrobial effects. This study aimed to evaluate the effect of rosmarinic acid against CP-induced nephrotoxicity.
Materials and Methods: Eighteen male Sprague Dawley rats were randomly divided into 3 equal groups; Sham group (n=6): 0.9% saline solution/8 days/oral gavage + 0.9% saline solution/8th day/intraperitoneal, CP group (n=6): 0.9% saline solution/8 days/oral gavage + 200 mg/kg/8th day/intraperitoneal CP, and CP+RA group (n=6): 100 mg/kg/8 days/oral gavage RA + 200 mg/kg/8th day/intraperitoneal CP was applied. Hematoxylin and Eosin, Periodic Acid-Schiff, and Masson’s Trichrome staining were performed on the collected tissues
Results: Histopathological evaluation revealed tubular atrophy, glomerular damage, vascular congestion, vacuolization, and interstitial inflammation in the CP group. Histopathological scores were significantly lower in the CP+RA group compared to the CP group. Intertubular fibrosis was observed in the CP group compared to the Sham group. Fibrosis decreased with rosmarinic acid. PAS-stained sections from the CP group showed tubular epithelial vacuolization, brush border, and basal membrane disruption. These findings decreased with rosmarinic acid. The increased blood urea nitrogen level in the CP group was lower in the CP+RA group, while the decreased SOD level in the CP group was higher in the CP+RA group.
Conclusion: RA has protective effects against CP causing tubular atrophy, glomerular damage, vascular congestion, vacuolization, and interstitial inflammation in the kidney
{"title":"Effects of rosmarinic acid on cyclophosphamide-induced nephrotoxicity in rats","authors":"Dilan ÇETİNAVCI, Engin YENİLMEZ, Ayşe Firuze BIYIK, Ahmet ALVER, Neslihan SAĞLAM","doi":"10.17826/cumj.1317508","DOIUrl":"https://doi.org/10.17826/cumj.1317508","url":null,"abstract":"Purpose: Cyclophosphamide (CP) is an antineoplastic agent. It is used in the treatment of many types of cancer. Rosmarinic acid (RA) exhibits remarkable biological activities such as anti-inflammatory, antitumor, antibacterial, and antimicrobial effects. This study aimed to evaluate the effect of rosmarinic acid against CP-induced nephrotoxicity.
 Materials and Methods: Eighteen male Sprague Dawley rats were randomly divided into 3 equal groups; Sham group (n=6): 0.9% saline solution/8 days/oral gavage + 0.9% saline solution/8th day/intraperitoneal, CP group (n=6): 0.9% saline solution/8 days/oral gavage + 200 mg/kg/8th day/intraperitoneal CP, and CP+RA group (n=6): 100 mg/kg/8 days/oral gavage RA + 200 mg/kg/8th day/intraperitoneal CP was applied. Hematoxylin and Eosin, Periodic Acid-Schiff, and Masson’s Trichrome staining were performed on the collected tissues 
 Results: Histopathological evaluation revealed tubular atrophy, glomerular damage, vascular congestion, vacuolization, and interstitial inflammation in the CP group. Histopathological scores were significantly lower in the CP+RA group compared to the CP group. Intertubular fibrosis was observed in the CP group compared to the Sham group. Fibrosis decreased with rosmarinic acid. PAS-stained sections from the CP group showed tubular epithelial vacuolization, brush border, and basal membrane disruption. These findings decreased with rosmarinic acid. The increased blood urea nitrogen level in the CP group was lower in the CP+RA group, while the decreased SOD level in the CP group was higher in the CP+RA group. 
 Conclusion: RA has protective effects against CP causing tubular atrophy, glomerular damage, vascular congestion, vacuolization, and interstitial inflammation in the kidney","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"128 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ertuğrul BAYRAM, Burak METE, Pakize İrem KAHRAMANOĞLU, Ebru MELEKOĞLU, Mehmet TURKER, İ. Oğuz KARA
Purpose: This study aimed to compare the effectiveness and toxicity of neoadjuvant dual Human epidermal growth factor receptor-2 (HER-2) blockade combined with chemotherapy in advanced breast cancer.
Materials and Methods: Patients with HER2-positive breast cancer who received trastuzumab (T)+ pertuzumab (P) with weekly neoadjuvant paclitaxel or docetaxel were included in the study. Patients’ age, clinical stage, histological reports, ki-67 index, toxicity profiles, and the state of the pathological and radiological response following neoadjuvant therapy were evaluated.
Results: All 40 patients were women (mean age 50.9) and the overall rate of pathological complete responses was 62.5% (25/40). The rates of non-responsive patients and grade 2 neuropathy were statistically significantly higher in the group receiving P+T+Weekly Paclitaxel. When SUV values were analyzed based on hormone positivity, it was found that they decreased dramatically in both groups and were statistically significant. The logistic regression analysis developed to predict the precise response status to therapy was found to be significant.
Conclusion: When comparing the agents used in dual HER-2 targeted therapies, patient response rates and toxicity profiles may differ. Ductal carcinoma in situ (DCIS) and molecular subtype were found to be significant variables in the developed logistic regression model.
{"title":"Evaluation of clinical and pathological responses of HER-2 positive locally advanced breast cancer patients after neoadjuvant therapy and surgery","authors":"Ertuğrul BAYRAM, Burak METE, Pakize İrem KAHRAMANOĞLU, Ebru MELEKOĞLU, Mehmet TURKER, İ. Oğuz KARA","doi":"10.17826/cumj.1343734","DOIUrl":"https://doi.org/10.17826/cumj.1343734","url":null,"abstract":"Purpose: This study aimed to compare the effectiveness and toxicity of neoadjuvant dual Human epidermal growth factor receptor-2 (HER-2) blockade combined with chemotherapy in advanced breast cancer.
 Materials and Methods: Patients with HER2-positive breast cancer who received trastuzumab (T)+ pertuzumab (P) with weekly neoadjuvant paclitaxel or docetaxel were included in the study. Patients’ age, clinical stage, histological reports, ki-67 index, toxicity profiles, and the state of the pathological and radiological response following neoadjuvant therapy were evaluated. 
 Results: All 40 patients were women (mean age 50.9) and the overall rate of pathological complete responses was 62.5% (25/40). The rates of non-responsive patients and grade 2 neuropathy were statistically significantly higher in the group receiving P+T+Weekly Paclitaxel. When SUV values were analyzed based on hormone positivity, it was found that they decreased dramatically in both groups and were statistically significant. The logistic regression analysis developed to predict the precise response status to therapy was found to be significant.
 Conclusion: When comparing the agents used in dual HER-2 targeted therapies, patient response rates and toxicity profiles may differ. Ductal carcinoma in situ (DCIS) and molecular subtype were found to be significant variables in the developed logistic regression model.","PeriodicalId":10748,"journal":{"name":"Cukurova Medical Journal","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136277838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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