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Ice crystallization and structural changes in cheese during freezing and frozen storage: implications for functional properties. 奶酪在冷冻和冷藏过程中的冰结晶和结构变化:对功能特性的影响。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2023-11-16 DOI: 10.1080/10408398.2023.2277357
Digvijay, Alan L Kelly, Prabin Lamichhane

Temperature-mediated preservation techniques offer a simple, scalable, effective, and fairly efficient method of long-term storage of food products. In order to ensure the uninterrupted availability of cheese across the globe, a critical understanding of its techno-functional properties as affected by freezing and frozen storage is essential. Detailed studies of temperature-mediated molecular dynamics are available for relatively simpler and homogeneous systems like pure water, proteins, and carbohydrates. However, for heterogeneous systems like cheese, inter-component interactions at sub-zero temperatures have not been extensively covered. Ice crystallization during freezing causes dehydration of caseins and the formation of concentration gradients within the cheese matrix, causing undesirable changes in texture-functional attributes, but findings vary due to experimental conditions. A suitable combination of sample size, freezing rate, aging, and tempering can extend the shelf life of high- and low-moisture Mozzarella cheese. However, limited studies on other cheeses suggest that effects and suitability differ by cheese type, in most cases adversely affecting texture and functional attributes. This review presents an overview of the understanding of the effects of refrigeration, freezing techniques, and frozen storage on structural components of cheese, most prominently Mozzarella cheese, and the corresponding impact on microstructure and functionality. Also included are the mechanism of ice formation and relevant mathematical models for estimation of the thermophysical properties of cheese to assist in designing optimized schemes for their frozen storage. The review also highlights the lack of unanimity in critical understanding concerning the effect of freezing on the long-term storage of Mozzarella cheese with respect to its functionality.

温度介导保存技术提供了一种简单、可扩展、有效且相当高效的食品长期储存方法。为了确保奶酪在全球范围内的不间断供应,对其受冷冻和冷冻储存影响的技术功能特性的批判性理解至关重要。温度介导的分子动力学的详细研究可用于相对简单和均匀的系统,如纯水,蛋白质和碳水化合物。然而,对于像奶酪这样的异质系统,组分间在零下温度下的相互作用还没有得到广泛的研究。冷冻过程中的冰结晶会导致酪蛋白脱水,并在奶酪基质中形成浓度梯度,从而导致质地功能属性的不良变化,但实验结果因实验条件而异。适当的样品大小、冷冻速率、陈化和回火组合可以延长高水分和低水分马苏里拉奶酪的保质期。然而,对其他奶酪的有限研究表明,奶酪的效果和适用性因奶酪类型而异,在大多数情况下会对质地和功能属性产生不利影响。这篇综述概述了冷藏、冷冻技术和冷冻储存对奶酪(尤其是马苏里拉奶酪)结构成分的影响,以及对其微观结构和功能的相应影响。还包括冰的形成机制和估算奶酪热物理性质的相关数学模型,以帮助设计其冷冻储存的优化方案。该综述还强调,在冷冻对马苏里拉奶酪长期储存的影响方面,对其功能的批判性理解缺乏一致。
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引用次数: 0
Degradation mechanism and development of detection technologies of ATP-related compounds in aquatic products: recent advances and remaining challenges. 水产品中ATP相关化合物的降解机制和检测技术的发展:最新进展和剩余挑战。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2023-10-19 DOI: 10.1080/10408398.2023.2267690
Bohan Chen, Qi Yan, Dapeng Li, Jing Xie

The degradation of ATP-related compounds is an important biochemical process that reflects the freshness of aquatic products after death. There has been considerable interest in investigating the factors affecting the degradation of ATP-related compounds in aquatic products and in developing techniques to detect them. This review provides the latest knowledge on the degradation mechanisms of ATP-related compounds during the storage of aquatic products and discusses the latest advances in ATP-related compound detection techniques. The degradation mechanisms discussed include mainly degradation pathways, endogenous enzymes, and microbial mechanisms of action. Microbial activity is the main reason for the degradation of IMP and related products during the mid to late storage of aquatic products, mainly through the related enzymes produced by microorganisms. Further elucidation of the degradation mechanisms of ATP-related compounds provides new ideas for quality control techniques in raw aquatic products during storage. The development of new technologies for the detection of ATP-related compounds has become a significant area of research. And, biosensors further improve the efficiency and accuracy of detection and have potential application prospects. The development of biosensor back-end modalities (test strips, fluorescent probes, and artificial intelligence) has accelerated the practical application of biosensors for the detection of ATP-related compounds.

ATP相关化合物的降解是一个重要的生化过程,反映了水产品死后的新鲜程度。人们对研究影响水产品中ATP相关化合物降解的因素以及开发检测技术非常感兴趣。这篇综述提供了有关水产品储存过程中ATP相关化合物降解机制的最新知识,并讨论了ATP相关化合物检测技术的最新进展。所讨论的降解机制主要包括降解途径、内源性酶和微生物作用机制。微生物活性是水产品中后期储存过程中IMP及相关产品降解的主要原因,主要是通过微生物产生的相关酶。进一步阐明ATP相关化合物的降解机制,为水产品贮藏过程中的质量控制技术提供了新的思路。开发检测ATP相关化合物的新技术已成为一个重要的研究领域。生物传感器进一步提高了检测的效率和准确性,具有潜在的应用前景。生物传感器后端模式(测试条、荧光探针和人工智能)的发展加速了生物传感器在检测ATP相关化合物方面的实际应用。
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引用次数: 0
Bacterial microbiota in different types of processed meat products: diversity, adaptation, and co-occurrence. 不同类型加工肉制品中的细菌微生物群:多样性、适应性和共现性。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2023-10-31 DOI: 10.1080/10408398.2023.2272770
Qianli Huang, Huijuan Zhang, Li Zhang, Baocai Xu

As a double-edged sword, some bacterial microbes can improve the quality and shelf life of meat products, but others mainly responsible for deterioration of the safety and quality of meat products. This review aims to present a landscape of the bacterial microbiota in different types of processed meat products. After demonstrating a panoramic view of the bacterial genera in meat products, the diversity of bacterial microbiota was evaluated in two dimensions, namely different types of processed meat products and different meats. Then, the influence of environmental factors on bacterial communities was evaluated according to the storage temperature, packaging conditions, and sterilization methods. Furthermore, microbes are not independent. To explore interactions among those genera, co-occurrence patterns were examined. In these respects, this review highlighted the recent advances in fundamental principles that underlie the environmental adaption tricks and why some species tend to occur together frequently, such as metabolic cross-feeding, co-aggregate at microscale, and the intercellular signaling system. Further investigations are required to unveil the underlying molecular mechanisms that govern microbial community systems, ultimately contributing to developing new strategies to harness beneficial microorganisms and control harmful microorganisms.

作为一把双刃剑,一些细菌微生物可以提高肉制品的质量和保质期,但另一些细菌微生物则是导致肉制品安全性和质量恶化的主要原因。这篇综述旨在介绍不同类型加工肉制品中的细菌微生物群。在展示了肉制品中细菌属的全景后,从两个维度评估了细菌微生物群的多样性,即不同类型的加工肉制品和不同的肉类。然后,根据储存温度、包装条件和杀菌方法,评估环境因素对细菌群落的影响。此外,微生物并不是独立的。为了探索这些属之间的相互作用,研究了共现模式。在这些方面,这篇综述强调了环境适应技巧背后的基本原理的最新进展,以及为什么一些物种经常出现在一起,例如代谢交叉喂养、微尺度共聚集和细胞间信号系统。需要进一步的研究来揭示控制微生物群落系统的潜在分子机制,最终有助于开发利用有益微生物和控制有害微生物的新策略。
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引用次数: 0
Lipid emulsion structure, digestion behavior, physiology, and health: a scoping review and future directions. 脂质乳液结构、消化行为、生理学和健康:范围综述和未来方向。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2023-11-10 DOI: 10.1080/10408398.2023.2273448
Erik Dassoff, Arshia Shireen, Amanda Wright

Research investigating the effects of the food matrix on health is needed to untangle many unresolved questions in nutritional science. Emulsion structure plays a fundamental role in this inquiry; however, the effects of oil-in-water emulsion structure on broad metabolic, physiological, and health-related outcomes have not been comprehensively reviewed. This systematic scoping review targets this gap and examines methodological considerations for the field of relating food structure and health. MEDLINE, Web of Science, and CAB Direct were searched from inception to December 2022, returning 3106 articles, 52 of which were eligible for inclusion. Many investigated emulsion lipid droplet size and/or gastric colloidal stability and their relation to postprandial weight-loss-related outcomes. The present review also identifies numerous novel relationships between emulsion structures and health-related outcomes. "Omics" endpoints present an exciting avenue for more comprehensive analysis in this area, yet interpretation remains difficult. Identifying valid surrogate biomarkers for long-term outcomes and disease risk will be a turning point for food structure research, leading to breakthroughs in the pace and utility of research that generates advancements in health. The review's findings and recommendations aim to support new hypotheses, future trial design, and evidence-based emulsion design for improved health and well-being.

需要研究食物基质对健康的影响,以解决营养科学中许多尚未解决的问题。乳液结构在这一研究中起着基础性作用;然而,水包油乳液结构对广泛的代谢、生理和健康结果的影响尚未得到全面的综述。这项系统的范围界定审查针对这一差距,并审查了相关食品结构和健康领域的方法考虑因素。MEDLINE、Web of Science和CAB Direct从成立到2022年12月进行了搜索,返回了3106篇文章,其中52篇符合入选条件。许多人研究了乳液脂滴大小和/或胃胶体稳定性及其与餐后减肥相关结果的关系。本综述还确定了乳液结构与健康相关结果之间的许多新关系。“奥密克戎”终点为该领域的更全面分析提供了一条令人兴奋的途径,但解释仍然很困难。确定长期结果和疾病风险的有效替代生物标志物将是食物结构研究的转折点,从而在研究的速度和效用方面取得突破,从而促进健康。该综述的发现和建议旨在支持新的假设、未来的试验设计和循证乳液设计,以改善健康和幸福感。
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引用次数: 0
Nutritional contributions and processability of pasta made from climate-smart, sustainable crops: A critical review. 气候智能、可持续作物制成的意大利面的营养贡献和加工性:一项重要综述。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2023-11-08 DOI: 10.1080/10408398.2023.2271952
P Pinel, M N Emmambux, C Bourlieu, V Micard

Total or partial replacement of traditional durum wheat semolina (DWS) by alternative flours, such as legumes or wholegrain cereals in pasta improves their nutritional quality and can make them interesting vector for fortification. Climate-smart gluten-free (C-GF) flours, such as legumes (bambara groundnut, chickpea, cowpea, faba bean, and pigeon pea), some cereals (amaranth, teff, millet, and sorghum), and tubers (cassava and orange fleshed sweet potato), are of high interest to face ecological transition and develop sustainable food systems. In this review, an overview and a critical analysis of their nutritional potential for pasta production and processing conditions are undertaken. Special emphasis is given to understanding the influence of formulation and processing on techno-functional and nutritional (starch and protein digestibility) properties. Globally C-GF flours improve pasta protein quantity and quality, fibers, and micronutrients contents while keeping a low glycemic index and increasing protein digestibility. However, their use introduces anti-nutritional factors and could lead to the alteration of their techno-functional properties (higher cooking losses, lower firmness, and variability in color in comparison to classical DWS pasta). Nevertheless, these alternative pasta remain more interesting in terms of nutritional and techno-functional quality than traditional maize and rice-based gluten free pasta.

用替代面粉(如意大利面中的豆类或全谷物)完全或部分取代传统的硬粒小麦粗面粉(DWS)可以提高其营养质量,并使其成为有趣的强化载体。气候智能无麸质(C-GF)面粉,如豆类(竹花生、鹰嘴豆、豇豆、蚕豆和鸽子豆)、一些谷物(苋、聚四氟乙烯、小米和高粱)和块茎(木薯和橙肉红薯),在面临生态转型和发展可持续粮食系统方面备受关注。在这篇综述中,对它们在意大利面生产和加工条件下的营养潜力进行了概述和批判性分析。特别强调了解配方和加工对技术功能和营养(淀粉和蛋白质消化率)特性的影响。在全球范围内,C-GF面粉提高了意大利面蛋白质的数量和质量、纤维和微量营养素的含量,同时保持了低血糖指数并提高了蛋白质的消化率。然而,它们的使用引入了抗营养因子,并可能导致其技术功能特性的改变(与经典DWS意大利面相比,烹饪损失更高,硬度更低,颜色多变)。尽管如此,与传统的玉米和大米无麸质意大利面相比,这些替代意大利面在营养和技术功能质量方面仍然更有趣。
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引用次数: 0
Interactions between dietary cholesterol and intestinal flora and their effects on host health. 膳食胆固醇与肠道菌群的相互作用及其对宿主健康的影响。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2023-11-10 DOI: 10.1080/10408398.2023.2276883
Yan Liu, Hang Xiao, Zhangtie Wang, Qiannan Pan, Xi Zhao, Baiyi Lu

The interactions between dietary cholesterol and intestinal microbiota strongly affect host health. In recent years, relevant studies have greatly advanced this field and need to be summarized to deepen the understanding of dietary cholesterol-intestinal microbiota interactions and their effects on host health. This review covers the most recent frontiers on the effects of dietary cholesterol on the intestinal microbiota and its metabolites, the metabolism of cholesterol by the intestinal microbiota, and the effects of the interactions on host health. Several animal-feeding studies reported that dietary cholesterol altered different intestinal microbiota in the body, while mainly causing alterations in intestinal microbial metabolites such as bile acids, short-chain fatty acids, and tryptophan derivatives. Alterations in these metabolites may be a novel mechanism mediating cholesterol-related diseases. The cholesterol microbial metabolite, coprostanol, has a low absorption rate and is excreted in the feces. Thus, microbial conversion of cholesterol-to-coprostanol may be an important way of cholesterol-lowering by the organism. Cholesterol-3-sulfate is a recently discovered microbial metabolite of cholesterol, mainly metabolized by Bacteroides containing the Bt_0416 gene. Its effects on host health have been preliminarily characterized and are mainly related to immune modulation and repair of the intestinal epithelium.

膳食胆固醇和肠道微生物群之间的相互作用强烈影响宿主的健康。近年来,相关研究极大地推动了这一领域的发展,需要对其进行总结,以加深对膳食胆固醇与肠道微生物群相互作用及其对宿主健康的影响的理解。这篇综述涵盖了饮食胆固醇对肠道微生物群及其代谢产物的影响、肠道微生物群对胆固醇的代谢以及相互作用对宿主健康的影响的最新前沿。几项动物喂养研究报告称,膳食胆固醇改变了体内不同的肠道微生物群,同时主要导致肠道微生物代谢产物如胆汁酸、短链脂肪酸和色氨酸衍生物的改变。这些代谢产物的改变可能是介导胆固醇相关疾病的一种新机制。胆固醇微生物代谢产物粪甾醇的吸收率较低,并通过粪便排出。因此,微生物将胆固醇转化为粪甾醇可能是生物体降低胆固醇的重要途径。胆固醇-3-硫酸盐是最近发现的胆固醇的微生物代谢产物,主要由含有Bt_0416基因的拟杆菌代谢。其对宿主健康的影响已得到初步表征,主要与免疫调节和肠上皮修复有关。
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引用次数: 0
Polyphenolic profile, processing impact, and bioaccessibility of apple fermented products. 苹果发酵产品的多酚特征、加工影响和生物可及性。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2024-01-22 DOI: 10.1080/10408398.2023.2277353
K Küçükgöz, J Echave, P Garcia-Oliveira, S Seyyedi-Mansour, P Donn, J Xiao, Monika Trząskowska, M A Prieto

Health-promoting foods have become increasingly popular due to intensified consumer interest and awareness of illnesses. There is a global market for apple fruits, which are affordable, nutritious, tasty, and produced in large quantities for direct consumption as well as food processing to make derived products. The food matrix of apples is suitable for fermentation, besides containing a high amount of phenolics and polyphenols. Fermentation of apples is one of the most common methods of preserving apple fruit and its byproducts. With different fermentation techniques, apple fruit can be used to make a wide range of products, such as fermented apple juice, cider, liqueurs, apple cider, apple vinegar and fermented apple solids, because it is not only a low-cost and simple method of processing the fruit, but it can also sometimes increase the bioavailability of nutrients and the levels of components that can improve health and sensory quality. To understand the health benefits of food products and how the fermentation process impacts polyphenols, it is also crucial to observe the effects of digestion on polyphenol bioaccessibility. Polyphenolic profile changes can be observed via both in vitro and in vivo digestion methods; however, in vitro digestion methods have the advantage of observing every step of gastrointestinal track effects and have less cost as well. In this review, the polyphenolic profile, processing impact, and bioaccessibility of apple-fermented products is assessed, with most available studies showing polyphenol profiles and bioaccessibility in apple varieties and fermented apple products.

由于消费者的兴趣和对疾病的认识不断提高,促进健康的食品越来越受欢迎。苹果果实价格低廉、营养丰富、味道鲜美、产量大,既可直接食用,也可用于食品加工,制成衍生产品。苹果的食物基质适合发酵,此外还含有大量酚类物质和多酚。苹果发酵是保存苹果果实及其副产品最常用的方法之一。通过不同的发酵技术,苹果果实可制成多种产品,如发酵苹果汁、苹果酒、利口酒、苹果汁、苹果醋和发酵苹果固体,因为它不仅是一种低成本、简单的果实加工方法,有时还能提高营养物质的生物利用率和成分水平,从而改善健康和感官质量。要了解食品对健康的益处以及发酵过程对多酚的影响,观察消化对多酚生物利用率的影响也至关重要。体外和体内消化方法都能观察到多酚类物质的变化;不过,体外消化方法的优点是能观察到胃肠道每一步的影响,而且成本较低。本综述对苹果发酵产品的多酚特征、加工影响和生物可及性进行了评估,大多数现有研究显示了苹果品种和发酵苹果产品的多酚特征和生物可及性。
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引用次数: 0
Recent advances in taste transduction mechanism, analysis methods and strategies employed to improve the taste of taste peptides. 味觉肽的味觉转导机制、分析方法及改善味觉的策略研究进展。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2023-11-15 DOI: 10.1080/10408398.2023.2280246
Chunyong Song, Zhijun Wang, Hanqi Li, Wenhong Cao, Zhongqin Chen, Huina Zheng, Jialong Gao, Haisheng Lin, Guoping Zhu

Taste peptides are oligopeptides that enhance both aroma and taste of food, and they are classified into five categories based on their taste characteristics: salty, sour, umami, sweet, bitter, and kokumi peptide. Recently, taste peptides have attracted the attention of several fields of research in food science and commercial applications. However, research on taste receptors of taste peptides and their taste transduction mechanisms are not clearly understood and we present a comprehensive review about these topics here. This review covers the aspects of taste peptides perceived by their receptors in taste cells, the proposed transduction pathway, as well as structural features of taste peptides. Apart from traditional methods, molecular docking, peptidomic analysis, cell and animal models and taste bud biosensors can be used to explore the taste mechanism of taste peptides. Furthermore, synergistic effect, Maillard reaction, structural modifications and changing external environment are employed to improve the taste of taste peptides. Consequently, we discussed the current challenges and future trends in taste peptide research. Based on the summarized developments, taste peptides derived from food proteins potentially appear to be important taste substances. Their applications meet the principles of "safe, nutritious and sustainable" in food development.

味觉肽是增强食物香气和口感的寡肽,根据其味觉特征分为咸、酸、鲜、甜、苦、厚肽五类。近年来,味觉肽在食品科学和商业应用领域的研究受到了广泛的关注。然而,关于味觉肽的味觉受体及其味觉转导机制的研究尚不清楚,本文就这方面的研究进展作一综述。本文综述了味觉细胞中受体感知的味觉肽、提出的味觉肽转导途径以及味觉肽的结构特征。除传统方法外,还可以通过分子对接、肽组学分析、细胞和动物模型、味蕾生物传感器等方法来探索味觉肽的味觉机制。此外,还通过协同效应、美拉德反应、结构修饰和外部环境的变化来改善味觉肽的口感。因此,我们讨论了味觉肽研究的当前挑战和未来趋势。综上所述,从食物蛋白中提取的味觉肽有可能成为重要的味觉物质。它们的应用符合食品开发中的“安全、营养和可持续”原则。
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引用次数: 0
New perspectives on different Sacha inchi seed oil extractions and its applications in the food and cosmetic industries. 不同核桃籽油提取物及其在食品和化妆品工业中的应用的新进展。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2023-11-11 DOI: 10.1080/10408398.2023.2276882
Wen-Chien Lu, Chien-Shan Chiu, Yung-Jia Chan, Amanda Tresiliana Mulio, Po-Hsien Li

Sacha inchi oil is growing in demand worldwide owing to its high fatty acid content of linolenic acid (44.30%-51.62%) and linoleic acid (34.08%-36.13%). In addition, Sacha inchi oil also contains phytosterols, such as stigmasterols (346- 456 μg/g), sitosterols (435-563 μg/g), and campesterols (10.47% ± 4.36%). Its main tocopherol is gamma-tocopherol (120.41-125.69 mg/100 g). The antinutrients in Sacha inchi seeds can be reduced by roasting prior to extraction. Various extractions, including both conventional and novel methods, have been used to extract Sacha inchi oil. However, the variety of extraction methods and origins of the seeds change the nutrient profiles, antinutrient content, and physicochemical properties. Incorporation of Sacha inchi oil into food products can increase its nutritional value, and it works as a moisturizing agent in cosmetic products. To obtain Sacha inchi oil with the desired properties and nutritional profile, this review summarizes the effects of different Sacha inchi seed oil extraction methods and processes on chemical compounds, antinutrient content, and physicochemical properties, including their potential and recent applications in food and cosmetic industries.

由于其亚麻酸(44.30%-51.62%)和亚油酸(34.08%-36.13%)的高脂肪酸含量,世界范围内对核桃油的需求正在增长。此外,茶籽油还含有豆甾醇(346 ~ 456 μg/g)、谷甾醇(435 ~ 563 μg/g)、油菜甾醇(10.47%±4.36%)等植物甾醇。其主要的生育酚是γ -生育酚(120.41-125.69毫克/100克)。在提取之前,可以通过烘烤来降低印度茶树种子中的抗营养成分。各种提取方法,包括传统方法和新方法,已被用于提取印度树油。然而,各种提取方法和种子的来源改变了其营养成分、抗营养成分和理化性质。将印度茶树油加入食品中可以增加其营养价值,它还可以作为化妆品的保湿剂。为了获得具有理想性能和营养成分的印度树籽油,本文综述了不同提取方法和工艺对印度树籽油的化学成分、抗营养成分和理化性质的影响,以及它们在食品和化妆品工业中的应用前景和前景。
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引用次数: 0
Plant-derived rennet: research progress, novel strategies for their isolation, identification, mechanism, bioactive peptide generation, and application in cheese manufacturing. 植物来源的凝乳酶:研究进展、分离、鉴定的新策略、机制、生物活性肽的产生以及在奶酪生产中的应用。
IF 7.3 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2025-01-01 Epub Date: 2023-10-30 DOI: 10.1080/10408398.2023.2275295
Xueting Zhang, Liang Tao, Guangqiang Wei, Min Yang, Zilin Wang, Chongying Shi, Yanan Shi, Aixiang Huang

Rennet, an aspartate protease found in the stomach of unweaned calves, effectively cuts the peptide bond between Phe105-Met106 in κ-casein, hydrolyzing the casein micelles to coagulate the milk and is a crucial additive in cheese production. Rennet is one of the most used enzymes of animal origin in cheese making. However, using rennet al.one is insufficient to meet the increasing demand for cheese production worldwide. Numerous studies have shown that plant rennet can be an alternative to bovine rennet and exhibit a good renneting effect. Therefore, it is crucial and urgent to find a reliable plant rennet. Based on our team's research on rennet enzymes of plant origin, such as from Dregea sinensis Hemsl. and Moringa oleifer Lam., for more than ten years, this paper reviews the relevant literature on rennet sources, isolation, identification, rennet mechanism, functional active peptide screening, and application in cheese production. In addition, it proposes the various techniques for targeted isolation and identification of rennet and efficient screening of functionally active peptides, which show excellent prospects for development.

Rennet是一种在未断奶小牛胃中发现的天冬氨酸蛋白酶,它能有效地切断κ-酪蛋白中Phe105-Met106之间的肽键,水解酪蛋白胶束使牛奶凝结,是奶酪生产中的重要添加剂。Rennet是奶酪制作中最常用的动物源酶之一。然而,使用凝乳酶等不足以满足全球奶酪生产日益增长的需求。大量研究表明,植物凝乳酶可以替代牛凝乳酶,并表现出良好的凝乳效果。因此,寻找一种可靠的植物凝乳酶是至关重要和紧迫的。基于我们团队对植物来源的凝乳酶的研究,如中华鳖的凝乳酶。和Moringa oleifer Lam。,本文综述了十多年来有关凝乳酶的来源、分离、鉴定、凝乳酶机制、功能活性肽筛选及在干酪生产中的应用等方面的相关文献。此外,还提出了靶向分离鉴定凝乳酶和高效筛选功能活性肽的各种技术,具有良好的发展前景。
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引用次数: 0
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