首页 > 最新文献

Critical Reviews in Biochemistry and Molecular Biology最新文献

英文 中文
Distinct enzymatic strategies for de novo generation of disulfide bonds in membranes. 膜中从头生成二硫键的不同酶促策略。
IF 6.5 2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-02-01 Epub Date: 2023-04-25 DOI: 10.1080/10409238.2023.2201404
Weikai Li

Disulfide bond formation is a catalyzed reaction essential for the folding and stability of proteins in the secretory pathway. In prokaryotes, disulfide bonds are generated by DsbB or VKOR homologs that couple the oxidation of a cysteine pair to quinone reduction. Vertebrate VKOR and VKOR-like enzymes have gained the epoxide reductase activity to support blood coagulation. The core structures of DsbB and VKOR variants share the architecture of a four-transmembrane-helix bundle that supports the coupled redox reaction and a flexible region containing another cysteine pair for electron transfer. Despite considerable similarities, recent high-resolution crystal structures of DsbB and VKOR variants reveal significant differences. DsbB activates the cysteine thiolate by a catalytic triad of polar residues, a reminiscent of classical cysteine/serine proteases. In contrast, bacterial VKOR homologs create a hydrophobic pocket to activate the cysteine thiolate. Vertebrate VKOR and VKOR-like maintain this hydrophobic pocket and further evolved two strong hydrogen bonds to stabilize the reaction intermediates and increase the quinone redox potential. These hydrogen bonds are critical to overcome the higher energy barrier required for epoxide reduction. The electron transfer process of DsbB and VKOR variants uses slow and fast pathways, but their relative contribution may be different in prokaryotic and eukaryotic cells. The quinone is a tightly bound cofactor in DsbB and bacterial VKOR homologs, whereas vertebrate VKOR variants use transient substrate binding to trigger the electron transfer in the slow pathway. Overall, the catalytic mechanisms of DsbB and VKOR variants have fundamental differences.

二硫键的形成是一种催化反应,对分泌途径中蛋白质的折叠和稳定性至关重要。在原核生物中,二硫键是由 DsbB 或 VKOR 同源物生成的,它们将半胱氨酸对的氧化与醌还原结合起来。脊椎动物的 VKOR 和类似 VKOR 的酶具有环氧化物还原酶活性,支持血液凝固。DsbB 和 VKOR 变体的核心结构具有相同的结构,即一个支持耦合氧化还原反应的四跨膜螺旋束和一个含有另一对半胱氨酸的柔性区域,用于电子传递。尽管 DsbB 和 VKOR 变体的高分辨率晶体结构有很大的相似性,但它们最近的高分辨率晶体结构显示出显著的差异。DsbB 通过极性残基催化三元组激活半胱氨酸硫酸盐,这让人想起经典的半胱氨酸/丝氨酸蛋白酶。相比之下,细菌的 VKOR 同源物会形成一个疏水袋来激活硫代半胱氨酸。脊椎动物的 VKOR 和类 VKOR 保持了这个疏水袋,并进一步演化出两个强氢键,以稳定反应中间产物并提高醌的氧化还原电位。这些氢键对于克服环氧化物还原所需的较高能量屏障至关重要。DsbB 和 VKOR 变体的电子传递过程使用慢速和快速途径,但它们在原核细胞和真核细胞中的相对贡献可能不同。在 DsbB 和细菌 VKOR 同源物中,醌是一种紧密结合的辅助因子,而脊椎动物的 VKOR 变体则利用瞬时底物结合来触发慢速途径中的电子转移。总之,DsbB 和 VKOR 变体的催化机制有着本质的区别。
{"title":"Distinct enzymatic strategies for <i>de novo</i> generation of disulfide bonds in membranes.","authors":"Weikai Li","doi":"10.1080/10409238.2023.2201404","DOIUrl":"10.1080/10409238.2023.2201404","url":null,"abstract":"<p><p>Disulfide bond formation is a catalyzed reaction essential for the folding and stability of proteins in the secretory pathway. In prokaryotes, disulfide bonds are generated by DsbB or VKOR homologs that couple the oxidation of a cysteine pair to quinone reduction. Vertebrate VKOR and VKOR-like enzymes have gained the epoxide reductase activity to support blood coagulation. The core structures of DsbB and VKOR variants share the architecture of a four-transmembrane-helix bundle that supports the coupled redox reaction and a flexible region containing another cysteine pair for electron transfer. Despite considerable similarities, recent high-resolution crystal structures of DsbB and VKOR variants reveal significant differences. DsbB activates the cysteine thiolate by a catalytic triad of polar residues, a reminiscent of classical cysteine/serine proteases. In contrast, bacterial VKOR homologs create a hydrophobic pocket to activate the cysteine thiolate. Vertebrate VKOR and VKOR-like maintain this hydrophobic pocket and further evolved two strong hydrogen bonds to stabilize the reaction intermediates and increase the quinone redox potential. These hydrogen bonds are critical to overcome the higher energy barrier required for epoxide reduction. The electron transfer process of DsbB and VKOR variants uses slow and fast pathways, but their relative contribution may be different in prokaryotic and eukaryotic cells. The quinone is a tightly bound cofactor in DsbB and bacterial VKOR homologs, whereas vertebrate VKOR variants use transient substrate binding to trigger the electron transfer in the slow pathway. Overall, the catalytic mechanisms of DsbB and VKOR variants have fundamental differences.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10460286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10153293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sulfur incorporation into biomolecules: recent advances. 硫与生物分子的结合:最新进展。
IF 6.5 2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2022-10-01 Epub Date: 2022-11-20 DOI: 10.1080/10409238.2022.2141678
Shramana Chatterjee, Robert P Hausinger

Sulfur is an essential element for a variety of cellular constituents in all living organisms and adds considerable functionality to a wide range of biomolecules. The pathways for incorporating sulfur into central metabolites of the cell such as cysteine, methionine, cystathionine, and homocysteine have long been established. Furthermore, the importance of persulfide intermediates during the biosynthesis of thionucleotide-containing tRNAs, iron-sulfur clusters, thiamin diphosphate, and the molybdenum cofactor are well known. This review briefly surveys these topics while emphasizing more recent aspects of sulfur metabolism that involve unconventional biosynthetic pathways. Sacrificial sulfur transfers from protein cysteinyl side chains to precursors of thiamin and the nickel-pincer nucleotide (NPN) cofactor are described. Newer aspects of synthesis for lipoic acid, biotin, and other compounds are summarized, focusing on the requisite iron-sulfur cluster destruction. Sulfur transfers by using a noncore sulfide ligand bound to a [4Fe-4S] cluster are highlighted for generating certain thioamides and for alternative biosynthetic pathways of thionucleotides and the NPN cofactor. Thioamide formation by activating an amide oxygen atom via phosphorylation also is illustrated. The discussion of these topics stresses the chemical reaction mechanisms of the transformations and generally avoids comments on the gene/protein nomenclature or the sources of the enzymes. This work sets the stage for future efforts to decipher the diverse mechanisms of sulfur incorporation into biological molecules.

硫是所有生物体中各种细胞成分的必需元素,并为广泛的生物分子增加了相当大的功能。将硫纳入细胞中心代谢物(如半胱氨酸、蛋氨酸、半胱甘氨酸和同型半胱氨酸)的途径早已确立。此外,过硫中间体在含硫核苷酸的trna、铁硫簇、硫胺二磷酸和钼辅助因子的生物合成中的重要性是众所周知的。本文简要综述了这些主题,同时强调了涉及非常规生物合成途径的硫代谢的最新方面。牺牲硫从蛋白质半胱氨酸侧链转移到硫胺素和镍钳核苷酸(NPN)辅因子的前体。综述了硫辛酸、生物素和其他化合物合成的最新进展,重点介绍了铁硫团簇破坏的必要条件。利用与[4Fe-4S]簇结合的非核硫化物配体进行硫转移,可以生成某些硫酰胺,也可以替代硫核苷酸和NPN辅因子的生物合成途径。还说明了通过磷酸化激活酰胺氧原子形成硫酰胺。这些主题的讨论强调转化的化学反应机制,一般避免评论基因/蛋白质的命名法或酶的来源。这项工作为未来破译硫与生物分子结合的不同机制奠定了基础。
{"title":"Sulfur incorporation into biomolecules: recent advances.","authors":"Shramana Chatterjee, Robert P Hausinger","doi":"10.1080/10409238.2022.2141678","DOIUrl":"10.1080/10409238.2022.2141678","url":null,"abstract":"<p><p>Sulfur is an essential element for a variety of cellular constituents in all living organisms and adds considerable functionality to a wide range of biomolecules. The pathways for incorporating sulfur into central metabolites of the cell such as cysteine, methionine, cystathionine, and homocysteine have long been established. Furthermore, the importance of persulfide intermediates during the biosynthesis of thionucleotide-containing tRNAs, iron-sulfur clusters, thiamin diphosphate, and the molybdenum cofactor are well known. This review briefly surveys these topics while emphasizing more recent aspects of sulfur metabolism that involve unconventional biosynthetic pathways. Sacrificial sulfur transfers from protein cysteinyl side chains to precursors of thiamin and the nickel-pincer nucleotide (NPN) cofactor are described. Newer aspects of synthesis for lipoic acid, biotin, and other compounds are summarized, focusing on the requisite iron-sulfur cluster destruction. Sulfur transfers by using a noncore sulfide ligand bound to a [4Fe-4S] cluster are highlighted for generating certain thioamides and for alternative biosynthetic pathways of thionucleotides and the NPN cofactor. Thioamide formation by activating an amide oxygen atom via phosphorylation also is illustrated. The discussion of these topics stresses the chemical reaction mechanisms of the transformations and generally avoids comments on the gene/protein nomenclature or the sources of the enzymes. This work sets the stage for future efforts to decipher the diverse mechanisms of sulfur incorporation into biological molecules.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10033759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Milk glycan metabolism by intestinal bifidobacteria: insights from comparative genomics. 肠道双歧杆菌的牛奶糖代谢:比较基因组学的启示。
IF 6.5 2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2022-10-01 Epub Date: 2023-03-03 DOI: 10.1080/10409238.2023.2182272
Aleksandr A Arzamasov, Andrei L Osterman

Bifidobacteria are early colonizers of the human neonatal gut and provide multiple health benefits to the infant, including inhibiting the growth of enteropathogens and modulating the immune system. Certain Bifidobacterium species prevail in the gut of breastfed infants due to the ability of these microorganisms to selectively forage glycans present in human milk, specifically human milk oligosaccharides (HMOs) and N-linked glycans. Therefore, these carbohydrates serve as promising prebiotic dietary supplements to stimulate the growth of bifidobacteria in the guts of children suffering from impaired gut microbiota development. However, the rational formulation of milk glycan-based prebiotics requires a detailed understanding of how bifidobacteria metabolize these carbohydrates. Accumulating biochemical and genomic data suggest that HMO and N-glycan assimilation abilities vary remarkably within the Bifidobacterium genus, both at the species and strain levels. This review focuses on the delineation and genome-based comparative analysis of differences in respective biochemical pathways, transport systems, and associated transcriptional regulatory networks, providing a foundation for genomics-based projection of milk glycan utilization capabilities across a rapidly growing number of sequenced bifidobacterial genomes and metagenomic datasets. This analysis also highlights remaining knowledge gaps and suggests directions for future studies to optimize the formulation of milk-glycan-based prebiotics that target bifidobacteria.

双歧杆菌是人类新生儿肠道的早期定植菌,对婴儿的健康有多种益处,包括抑制肠道病原体的生长和调节免疫系统。母乳喂养的婴儿肠道中普遍存在某些双歧杆菌物种,这是因为这些微生物能够选择性地觅食母乳中的糖类,特别是母乳低聚糖(HMO)和N-连接糖。因此,这些碳水化合物是很有前景的益生元膳食补充剂,可刺激肠道微生物群发育受损儿童肠道中双歧杆菌的生长。然而,要合理配制基于牛奶聚糖的益生元,就必须详细了解双歧杆菌是如何代谢这些碳水化合物的。不断积累的生化和基因组数据表明,双歧杆菌属内的 HMO 和 N-糖同化能力在物种和菌株水平上都存在显著差异。本综述侧重于对各自生化途径、转运系统和相关转录调控网络的差异进行划分和基于基因组的比较分析,为基于基因组学的牛奶糖利用能力预测提供基础,这些预测涉及快速增长的双歧杆菌基因组测序和元基因组数据集。这项分析还强调了尚存在的知识空白,并为今后优化以双歧杆菌为目标的牛奶糖基益生元配方的研究提出了方向。
{"title":"Milk glycan metabolism by intestinal bifidobacteria: insights from comparative genomics.","authors":"Aleksandr A Arzamasov, Andrei L Osterman","doi":"10.1080/10409238.2023.2182272","DOIUrl":"10.1080/10409238.2023.2182272","url":null,"abstract":"<p><p>Bifidobacteria are early colonizers of the human neonatal gut and provide multiple health benefits to the infant, including inhibiting the growth of enteropathogens and modulating the immune system. Certain <i>Bifidobacterium</i> species prevail in the gut of breastfed infants due to the ability of these microorganisms to selectively forage glycans present in human milk, specifically human milk oligosaccharides (HMOs) and <i>N</i>-linked glycans. Therefore, these carbohydrates serve as promising prebiotic dietary supplements to stimulate the growth of bifidobacteria in the guts of children suffering from impaired gut microbiota development. However, the rational formulation of milk glycan-based prebiotics requires a detailed understanding of how bifidobacteria metabolize these carbohydrates. Accumulating biochemical and genomic data suggest that HMO and <i>N</i>-glycan assimilation abilities vary remarkably within the <i>Bifidobacterium</i> genus, both at the species and strain levels. This review focuses on the delineation and genome-based comparative analysis of differences in respective biochemical pathways, transport systems, and associated transcriptional regulatory networks, providing a foundation for genomics-based projection of milk glycan utilization capabilities across a rapidly growing number of sequenced bifidobacterial genomes and metagenomic datasets. This analysis also highlights remaining knowledge gaps and suggests directions for future studies to optimize the formulation of milk-glycan-based prebiotics that target bifidobacteria.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10051884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
G-quadruplexes in bacteria: insights into the regulatory roles and interacting proteins of non-canonical nucleic acid structures. 细菌中的G-四链体:对非规范核酸结构的调节作用和相互作用蛋白的见解。
IF 6.2 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2022-10-01 Epub Date: 2023-03-31 DOI: 10.1080/10409238.2023.2181310
Rachel R Cueny, Sarah D McMillan, James L Keck

G-quadruplexes (G4s) are highly stable, non-canonical DNA or RNA structures that can form in guanine-rich stretches of nucleic acids. G4-forming sequences have been found in all domains of life, and proteins that bind and/or resolve G4s have been discovered in both bacterial and eukaryotic organisms. G4s regulate a variety of cellular processes through inhibitory or stimulatory roles that depend upon their positions within genomes or transcripts. These include potential roles as impediments to genome replication, transcription, and translation or, in other contexts, as activators of genome stability, transcription, and recombination. This duality suggests that G4 sequences can aid cellular processes but that their presence can also be problematic. Despite their documented importance in bacterial species, G4s remain understudied in bacteria relative to eukaryotes. In this review, we highlight the roles of bacterial G4s by discussing their prevalence in bacterial genomes, the proteins that bind and unwind G4s in bacteria, and the processes regulated by bacterial G4s. We identify limitations in our current understanding of the functions of G4s in bacteria and describe new avenues for studying these remarkable nucleic acid structures.

G-四链体(G4s)是高度稳定的非经典DNA或RNA结构,可以在富含鸟嘌呤的核酸片段中形成。在生命的所有领域都发现了G4形成序列,在细菌和真核生物中都发现了结合和/或解析G4s的蛋白质。G4s通过抑制或刺激作用调节各种细胞过程,这取决于它们在基因组或转录物中的位置。这些包括作为基因组复制、转录和翻译的障碍的潜在作用,或者在其他情况下,作为基因组稳定性、转录和重组的激活剂。这种双重性表明G4序列可以帮助细胞过程,但它们的存在也可能是有问题的。尽管G4s在细菌物种中具有重要意义,但相对于真核生物,G4s在菌株中的研究仍然不足。在这篇综述中,我们通过讨论细菌G4s在细菌基因组中的普遍性、细菌中结合和释放G4s的蛋白质以及细菌G4s调节的过程来强调细菌G4s的作用。我们确定了目前对G4s在细菌中的功能理解的局限性,并描述了研究这些显著核酸结构的新途径。
{"title":"G-quadruplexes in bacteria: insights into the regulatory roles and interacting proteins of non-canonical nucleic acid structures.","authors":"Rachel R Cueny, Sarah D McMillan, James L Keck","doi":"10.1080/10409238.2023.2181310","DOIUrl":"10.1080/10409238.2023.2181310","url":null,"abstract":"<p><p>G-quadruplexes (G4s) are highly stable, non-canonical DNA or RNA structures that can form in guanine-rich stretches of nucleic acids. G4-forming sequences have been found in all domains of life, and proteins that bind and/or resolve G4s have been discovered in both bacterial and eukaryotic organisms. G4s regulate a variety of cellular processes through inhibitory or stimulatory roles that depend upon their positions within genomes or transcripts. These include potential roles as impediments to genome replication, transcription, and translation or, in other contexts, as activators of genome stability, transcription, and recombination. This duality suggests that G4 sequences can aid cellular processes but that their presence can also be problematic. Despite their documented importance in bacterial species, G4s remain understudied in bacteria relative to eukaryotes. In this review, we highlight the roles of bacterial G4s by discussing their prevalence in bacterial genomes, the proteins that bind and unwind G4s in bacteria, and the processes regulated by bacterial G4s. We identify limitations in our current understanding of the functions of G4s in bacteria and describe new avenues for studying these remarkable nucleic acid structures.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.2,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9775383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Overview of physiological, biochemical, and regulatory aspects of nitrogen fixation in Azotobacter vinelandii. 黄氏固氮固氮的生理、生化和调控方面综述。
IF 6.5 2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2022-10-01 DOI: 10.1080/10409238.2023.2181309
Julia S Martin Del Campo, Jack Rigsbee, Marcelo Bueno Batista, Florence Mus, Luis M Rubio, Oliver Einsle, John W Peters, Ray Dixon, Dennis R Dean, Patricia C Dos Santos

Understanding how Nature accomplishes the reduction of inert nitrogen gas to form metabolically tractable ammonia at ambient temperature and pressure has challenged scientists for more than a century. Such an understanding is a key aspect toward accomplishing the transfer of the genetic determinants of biological nitrogen fixation to crop plants as well as for the development of improved synthetic catalysts based on the biological mechanism. Over the past 30 years, the free-living nitrogen-fixing bacterium Azotobacter vinelandii emerged as a preferred model organism for mechanistic, structural, genetic, and physiological studies aimed at understanding biological nitrogen fixation. This review provides a contemporary overview of these studies and places them within the context of their historical development.

一个多世纪以来,了解大自然如何在环境温度和压力下完成惰性氮气的还原,形成可代谢的氨,一直是科学家们面临的挑战。这样的理解是实现生物固氮遗传决定因素向作物植物转移以及基于生物机制开发改进的合成催化剂的关键方面。在过去的30年里,自由生活的固氮细菌Azotobacter vinelandii成为了解生物固氮机制、结构、遗传和生理研究的首选模式生物。这篇综述提供了这些研究的当代概况,并将它们置于其历史发展的背景下。
{"title":"Overview of physiological, biochemical, and regulatory aspects of nitrogen fixation in <i>Azotobacter vinelandii</i>.","authors":"Julia S Martin Del Campo,&nbsp;Jack Rigsbee,&nbsp;Marcelo Bueno Batista,&nbsp;Florence Mus,&nbsp;Luis M Rubio,&nbsp;Oliver Einsle,&nbsp;John W Peters,&nbsp;Ray Dixon,&nbsp;Dennis R Dean,&nbsp;Patricia C Dos Santos","doi":"10.1080/10409238.2023.2181309","DOIUrl":"https://doi.org/10.1080/10409238.2023.2181309","url":null,"abstract":"<p><p>Understanding how Nature accomplishes the reduction of inert nitrogen gas to form metabolically tractable ammonia at ambient temperature and pressure has challenged scientists for more than a century. Such an understanding is a key aspect toward accomplishing the transfer of the genetic determinants of biological nitrogen fixation to crop plants as well as for the development of improved synthetic catalysts based on the biological mechanism. Over the past 30 years, the free-living nitrogen-fixing bacterium <i>Azotobacter vinelandii</i> emerged as a preferred model organism for mechanistic, structural, genetic, and physiological studies aimed at understanding biological nitrogen fixation. This review provides a contemporary overview of these studies and places them within the context of their historical development.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10034286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
2-5A-Mediated decay (2-5AMD): from antiviral defense to control of host RNA. 2-5A介导的衰变(2-5AMD):从抗病毒防御到控制宿主RNA。
IF 6.5 2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2022-10-01 Epub Date: 2023-03-20 DOI: 10.1080/10409238.2023.2181308
Eliza Prangley, Alexei Korennykh

Mammalian cells are exquisitely sensitive to the presence of double-stranded RNA (dsRNA), a molecule that they interpret as a signal of viral presence requiring immediate attention. Upon sensing dsRNA cells activate the innate immune response, which involves transcriptional mechanisms driving inflammation and secretion of interferons (IFNs) and interferon-stimulated genes (ISGs), as well as synthesis of RNA-like signaling molecules comprised of three or more 2'-5'-linked adenylates (2-5As). 2-5As were discovered some forty years ago and described as IFN-induced inhibitors of protein synthesis. The efforts of many laboratories, aimed at elucidating the molecular mechanism and function of these mysterious RNA-like signaling oligonucleotides, revealed that 2-5A is a specific ligand for the kinase-family endonuclease RNase L. RNase L decays single-stranded RNA (ssRNA) from viruses and mRNAs (as well as other RNAs) from hosts in a process we proposed to call 2-5A-mediated decay (2-5AMD). During recent years it has become increasingly recognized that 2-5AMD is more than a blunt tool of viral RNA destruction, but a pathway deeply integrated into sensing and regulation of endogenous RNAs. Here we present an overview of recently emerged roles of 2-5AMD in host RNA regulation.

哺乳动物细胞对双链RNA(dsRNA)的存在非常敏感,他们将这种分子解释为病毒存在的信号,需要立即关注。在感应到dsRNA细胞后,激活先天免疫反应,这涉及驱动炎症和干扰素(IFN)和干扰素刺激基因(ISG)分泌的转录机制,以及由三种或多种2'-5'-连接的腺苷酸(2-5As)组成的RNA样信号分子的合成。2-5As是大约四十年前发现的,被描述为IFN诱导的蛋白质合成抑制剂。许多实验室致力于阐明这些神秘的类RNA信号寡核苷酸的分子机制和功能,结果表明2-5A是激酶家族核酸内切酶RNase L的特异性配体。RNase L在我们提出称为2-5A介导的衰变(2-5AMD)的过程中衰变来自病毒的单链RNA(ssRNA)和来自宿主的mRNA(以及其他RNA)。近年来,人们越来越认识到2-5AMD不仅仅是一种破坏病毒RNA的钝工具,而是一种深入整合到内源性RNA传感和调节中的途径。在这里,我们概述了最近出现的2-5AMD在宿主RNA调节中的作用。
{"title":"2-5A-Mediated decay (2-5AMD): from antiviral defense to control of host RNA.","authors":"Eliza Prangley, Alexei Korennykh","doi":"10.1080/10409238.2023.2181308","DOIUrl":"10.1080/10409238.2023.2181308","url":null,"abstract":"<p><p>Mammalian cells are exquisitely sensitive to the presence of double-stranded RNA (dsRNA), a molecule that they interpret as a signal of viral presence requiring immediate attention. Upon sensing dsRNA cells activate the innate immune response, which involves transcriptional mechanisms driving inflammation and secretion of interferons (IFNs) and interferon-stimulated genes (ISGs), as well as synthesis of RNA-like signaling molecules comprised of three or more 2'-5'-linked adenylates (2-5As). 2-5As were discovered some forty years ago and described as IFN-induced inhibitors of protein synthesis. The efforts of many laboratories, aimed at elucidating the molecular mechanism and function of these mysterious RNA-like signaling oligonucleotides, revealed that 2-5A is a specific ligand for the kinase-family endonuclease RNase L. RNase L decays single-stranded RNA (ssRNA) from viruses and mRNAs (as well as other RNAs) from hosts in a process we proposed to call 2-5A-mediated decay (2-5AMD). During recent years it has become increasingly recognized that 2-5AMD is more than a blunt tool of viral RNA destruction, but a pathway deeply integrated into sensing and regulation of endogenous RNAs. Here we present an overview of recently emerged roles of 2-5AMD in host RNA regulation.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9678347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Creation and resolution of non-B-DNA structural impediments during replication. 在复制过程中产生并解决非 B-DNA 结构障碍。
IF 6.5 2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2022-08-01 Epub Date: 2022-09-28 DOI: 10.1080/10409238.2022.2121803
Christopher Mellor, Consuelo Perez, Julian E Sale

During replication, folding of the DNA template into non-B-form secondary structures provides one of the most abundant impediments to the smooth progression of the replisome. The core replisome collaborates with multiple accessory factors to ensure timely and accurate duplication of the genome and epigenome. Here, we discuss the forces that drive non-B structure formation and the evidence that secondary structures are a significant and frequent source of replication stress that must be actively countered. Taking advantage of recent advances in the molecular and structural biology of the yeast and human replisomes, we examine how structures form and how they may be sensed and resolved during replication.

在复制过程中,DNA 模板折叠成非 B 型二级结构是阻碍复制体顺利进行的最大障碍之一。核心复制体与多种辅助因子协作,确保及时准确地复制基因组和表观基因组。在这里,我们将讨论驱动非 B 结构形成的力量,以及二级结构是必须积极应对的重要且频繁的复制压力来源的证据。利用酵母和人类复制体分子和结构生物学的最新进展,我们研究了结构是如何形成的,以及在复制过程中如何感知和解决这些结构。
{"title":"Creation and resolution of non-B-DNA structural impediments during replication.","authors":"Christopher Mellor, Consuelo Perez, Julian E Sale","doi":"10.1080/10409238.2022.2121803","DOIUrl":"10.1080/10409238.2022.2121803","url":null,"abstract":"<p><p>During replication, folding of the DNA template into non-B-form secondary structures provides one of the most abundant impediments to the smooth progression of the replisome. The core replisome collaborates with multiple accessory factors to ensure timely and accurate duplication of the genome and epigenome. Here, we discuss the forces that drive non-B structure formation and the evidence that secondary structures are a significant and frequent source of replication stress that must be actively countered. Taking advantage of recent advances in the molecular and structural biology of the yeast and human replisomes, we examine how structures form and how they may be sensed and resolved during replication.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9185161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reversible and bidirectional signaling of notch ligands. 缺口配体的可逆和双向信号传导。
IF 6.5 2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2022-08-01 DOI: 10.1080/10409238.2022.2113029
Elenaé Vázquez-Ulloa, Kai-Lan Lin, Marcela Lizano, Cecilia Sahlgren

The Notch signaling pathway is a direct cell-cell communication system involved in a wide variety of biological processes, and its disruption is observed in several pathologies. The pathway is comprised of a ligand-expressing (sender) cell and a receptor-expressing (receiver) cell. The canonical ligands are members of the Delta/Serrate/Lag-1 (DSL) family of proteins. Their binding to a Notch receptor in a neighboring cell induces a conformational change in the receptor, which will undergo regulated intramembrane proteolysis (RIP), liberating the Notch intracellular domain (NICD). The NICD is translocated to the nucleus and promotes gene transcription. It has been demonstrated that the ligands can also undergo RIP and nuclear translocation, suggesting a function for the ligands in the sender cell and possible bidirectionality of the Notch pathway. Although the complete mechanism of ligand processing is not entirely understood, and its dependence on Notch receptors has not been ruled out. Also, ligands have autonomous functions beyond Notch activation. Here we review the concepts of reverse and bidirectional signalization of DSL proteins and discuss the characteristics that make them more than just ligands of the Notch pathway.

Notch信号通路是一个直接的细胞-细胞通讯系统,参与多种生物过程,在多种病理中观察到它的破坏。该途径由表达配体的细胞(发送者)和表达受体的细胞(接收者)组成。规范配体是Delta/Serrate/Lag-1 (DSL)蛋白家族的成员。它们与邻近细胞中的Notch受体结合,诱导受体发生构象变化,从而发生受调控的膜内蛋白水解(RIP),释放Notch胞内结构域(NICD)。NICD易位到细胞核并促进基因转录。研究表明,这些配体也可以经历RIP和核易位,这表明配体在发送细胞中起作用,并且可能是Notch通路的双向性。虽然配体加工的完整机制尚不完全清楚,但它对Notch受体的依赖性也不排除。此外,配体具有Notch激活之外的自主功能。在这里,我们回顾了DSL蛋白的反向和双向信号的概念,并讨论了使它们不仅仅是Notch途径的配体的特征。
{"title":"Reversible and bidirectional signaling of notch ligands.","authors":"Elenaé Vázquez-Ulloa,&nbsp;Kai-Lan Lin,&nbsp;Marcela Lizano,&nbsp;Cecilia Sahlgren","doi":"10.1080/10409238.2022.2113029","DOIUrl":"https://doi.org/10.1080/10409238.2022.2113029","url":null,"abstract":"<p><p>The Notch signaling pathway is a direct cell-cell communication system involved in a wide variety of biological processes, and its disruption is observed in several pathologies. The pathway is comprised of a ligand-expressing (sender) cell and a receptor-expressing (receiver) cell. The canonical ligands are members of the Delta/Serrate/Lag-1 (DSL) family of proteins. Their binding to a Notch receptor in a neighboring cell induces a conformational change in the receptor, which will undergo regulated intramembrane proteolysis (RIP), liberating the Notch intracellular domain (NICD). The NICD is translocated to the nucleus and promotes gene transcription. It has been demonstrated that the ligands can also undergo RIP and nuclear translocation, suggesting a function for the ligands in the sender cell and possible bidirectionality of the Notch pathway. Although the complete mechanism of ligand processing is not entirely understood, and its dependence on Notch receptors has not been ruled out. Also, ligands have autonomous functions beyond Notch activation. Here we review the concepts of reverse and bidirectional signalization of DSL proteins and discuss the characteristics that make them more than just ligands of the Notch pathway.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10613678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
What makes functional amyloids work? 是什么使功能性淀粉样蛋白起作用?
IF 6.5 2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2022-08-01 DOI: 10.1080/10409238.2022.2113030
Ansgar B Siemer

Although first described in the context of disease, cross-β (amyloid) fibrils have also been found as functional entities in all kingdoms of life. However, what are the specific properties of the cross-β fibril motif that convey biological function, make them especially suited for their particular purpose, and distinguish them from other fibrils found in biology? This review approaches these questions by arguing that cross-β fibrils are highly periodic, stable, and self-templating structures whose formation is accompanied by substantial conformational change that leads to a multimerization of their core and framing sequences. A discussion of each of these properties is followed by selected examples of functional cross-β fibrils that show how function is usually achieved by leveraging many of these properties.

虽然最初是在疾病的背景下被描述的,但交叉β(淀粉样蛋白)原纤维也被发现是所有生命领域的功能实体。然而,交叉β原纤维基序传达生物学功能的具体特性是什么,使它们特别适合于它们的特定用途,并将它们与生物学中发现的其他原纤维区分开来?这篇综述通过论证交叉β原纤维是高度周期性的、稳定的和自模板的结构,其形成伴随着大量的构象变化,导致其核心和框架序列的多聚合。对这些特性的讨论之后,选择功能性交叉β原纤维的例子,展示了通常如何通过利用许多这些特性来实现功能。
{"title":"What makes functional amyloids work?","authors":"Ansgar B Siemer","doi":"10.1080/10409238.2022.2113030","DOIUrl":"https://doi.org/10.1080/10409238.2022.2113030","url":null,"abstract":"<p><p>Although first described in the context of disease, cross-β (amyloid) fibrils have also been found as functional entities in all kingdoms of life. However, what are the specific properties of the cross-β fibril motif that convey biological function, make them especially suited for their particular purpose, and distinguish them from other fibrils found in biology? This review approaches these questions by arguing that cross-β fibrils are highly periodic, stable, and self-templating structures whose formation is accompanied by substantial conformational change that leads to a multimerization of their core and framing sequences. A discussion of each of these properties is followed by selected examples of functional cross-β fibrils that show how function is usually achieved by leveraging many of these properties.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588633/pdf/nihms-1829681.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10044607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Hypoxia-regulated microRNAs: the molecular drivers of tumor progression. 低氧调控的microrna:肿瘤进展的分子驱动因素。
IF 6.5 2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2022-08-01 DOI: 10.1080/10409238.2022.2088684
Sakunie Sawai, Pooi-Fong Wong, Thamil Selvee Ramasamy

Hypoxia is a common feature of the tumor microenvironment (TME) of nearly all solid tumors, leading to therapeutic failure. The changes in stiffness of the extracellular matrix (ECM), pH gradients, and chemical balance that contribute to multiple cancer hallmarks are closely regulated by intratumoral oxygen tension via its primary mediators, hypoxia-inducible factors (HIFs). HIFs, especially HIF-1α, influence these changes in the TME by regulating vital cancer-associated signaling pathways and cellular processes including MAPK/ERK, NF-κB, STAT3, PI3K/Akt, Wnt, p53, and glycolysis. Interestingly, research has revealed the involvement of epigenetic regulation by hypoxia-regulated microRNAs (HRMs) of downstream target genes involved in these signaling. Through literature search and analysis, we identified 48 HRMs that have a functional role in the regulation of 5 key cellular processes: proliferation, metabolism, survival, invasion and migration, and immunoregulation in various cancers in hypoxic condition. Among these HRMs, 17 were identified to be directly associated with HIFs which include miR-135b, miR-145, miR-155, miR-181a, miR-182, miR-210, miR-224, miR-301a, and miR-675-5p as oncomiRNAs, and miR-100-5p, miR-138, miR-138-5p, miR-153, miR-22, miR-338-3p, miR-519d-3p, and miR-548an as tumor suppressor miRNAs. These HRMs serve as a potential lead in the development of miRNA-based targeted therapy for advanced solid tumors. Future development of combined HIF-targeted and miRNA-targeted therapy is possible, which requires comprehensive profiling of HIFs-HRMs regulatory network, and improved formula of the delivery vehicles to enhance the therapeutic kinetics of the targeted cancer therapy (TCT) moving forward.

缺氧是几乎所有实体瘤肿瘤微环境(TME)的共同特征,导致治疗失败。细胞外基质(ECM)硬度、pH梯度和化学平衡的变化导致多种癌症特征,这些变化是由肿瘤内氧张力通过其主要介质缺氧诱导因子(hif)密切调节的。hif,尤其是HIF-1α,通过调节重要的癌症相关信号通路和细胞过程,包括MAPK/ERK、NF-κB、STAT3、PI3K/Akt、Wnt、p53和糖酵解,影响TME的这些变化。有趣的是,研究揭示了参与这些信号传导的下游靶基因的缺氧调节microRNAs (HRMs)参与表观遗传调控。通过文献检索和分析,我们确定了48个HRMs,它们在缺氧条件下调节各种癌症的5个关键细胞过程:增殖、代谢、生存、侵袭和迁移以及免疫调节中发挥功能作用。在这些HRMs中,有17个被确定与hif直接相关,其中miR-135b、miR-145、miR-155、miR-181a、miR-182、miR-210、miR-224、miR-301a和miR-675-5p为肿瘤mirna, miR-100-5p、miR-138、miR-138-5p、miR-153、miR-22、miR-338-3p、miR-519d-3p和miR-548an为肿瘤抑制mirna。这些HRMs在开发基于mirna的晚期实体瘤靶向治疗方面具有潜在的引领作用。未来hif靶向治疗和mirna靶向治疗的联合发展是可能的,这需要对hif - hrms调控网络进行全面分析,并改进递送载体的配方,以提高靶向癌症治疗(TCT)的治疗动力学。
{"title":"Hypoxia-regulated microRNAs: the molecular drivers of tumor progression.","authors":"Sakunie Sawai,&nbsp;Pooi-Fong Wong,&nbsp;Thamil Selvee Ramasamy","doi":"10.1080/10409238.2022.2088684","DOIUrl":"https://doi.org/10.1080/10409238.2022.2088684","url":null,"abstract":"<p><p>Hypoxia is a common feature of the tumor microenvironment (TME) of nearly all solid tumors, leading to therapeutic failure. The changes in stiffness of the extracellular matrix (ECM), pH gradients, and chemical balance that contribute to multiple cancer hallmarks are closely regulated by intratumoral oxygen tension <i>via</i> its primary mediators, hypoxia-inducible factors (HIFs). HIFs, especially HIF-1α, influence these changes in the TME by regulating vital cancer-associated signaling pathways and cellular processes including MAPK/ERK, NF-κB, STAT3, PI3K/Akt, Wnt, p53, and glycolysis. Interestingly, research has revealed the involvement of epigenetic regulation by hypoxia-regulated microRNAs (HRMs) of downstream target genes involved in these signaling. Through literature search and analysis, we identified 48 HRMs that have a functional role in the regulation of 5 key cellular processes: proliferation, metabolism, survival, invasion and migration, and immunoregulation in various cancers in hypoxic condition. Among these HRMs, 17 were identified to be directly associated with HIFs which include miR-135b, miR-145, miR-155, miR-181a, miR-182, miR-210, miR-224, miR-301a, and miR-675-5p as oncomiRNAs, and miR-100-5p, miR-138, miR-138-5p, miR-153, miR-22, miR-338-3p, miR-519d-3p, and miR-548an as tumor suppressor miRNAs. These HRMs serve as a potential lead in the development of miRNA-based targeted therapy for advanced solid tumors. Future development of combined HIF-targeted and miRNA-targeted therapy is possible, which requires comprehensive profiling of HIFs-HRMs regulatory network, and improved formula of the delivery vehicles to enhance the therapeutic kinetics of the targeted cancer therapy (TCT) moving forward.</p>","PeriodicalId":10794,"journal":{"name":"Critical Reviews in Biochemistry and Molecular Biology","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10668748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
期刊
Critical Reviews in Biochemistry and Molecular Biology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1