In this study, an anionic dyestuff bromophenol blue (BrPB) from the aqueous solution was removed by adsorption in the presence of bentonite (B), zeolite (Z) and graphene oxide (GO). The effects of pH, adsorbent amount, initial concentration of BrPB, temperature and time on adsorption were investigated. After the adsorption process, ultraviolet-visible region spectrophotometer (UV-VIS) was used to measure the amount of dyestuff remaining in the solution which is not adsorbed. It’s found that; the optimum values obtained for the adsorption process; the optimum pH value 5 for B and Z, 4 for GO; 0.03 g amount of adsorbent and 60 minutes for the equilibrium time. The compatibility of Langmiur and Freundlich isotherm models was found as Z> GO> B and GO> B> Z respectively. In terms of kinetics aspect, it was observed that all adsorbents were comply with pseudo-second order reaction kinetics. To be positive of ΔG° value for B and Z is of physical nature of adsorption, to be negative of ΔG° value for GO requires to be chemical nature of adsorption. When the obtained ΔH° values were examined, it was observed that the reaction was endothermic for B and Z and exothermic for GO. The negative value of ΔS° for B and Z adsorbent species indicates that the randomness between solid and liquid decreases, whereas the positive ΔS° value for GO indicates that the randomness between solid and liquid increases. The highest adsorption capacity value obtained after the measurements made under optimum conditions is 589 mg g-1 for GO and 15.7 and 19.8 mg g-1 for B and Z, respectively.
本研究在膨润土（B）、沸石（Z）和氧化石墨烯（GO）的存在下，通过吸附去除水溶液中的阴离子染料溴酚蓝（BrPB）。研究了 pH 值、吸附剂用量、BrPB 初始浓度、温度和时间对吸附的影响。吸附过程结束后，使用紫外-可见分光光度计（UV-VIS）测量溶液中未被吸附的染料残留量。结果发现，吸附过程中获得的最佳值为：B 和 Z 的最佳 pH 值为 5，GO 为 4；吸附剂用量为 0.03 克，平衡时间为 60 分钟。朗米尔和弗伦德里希等温线模型的兼容性分别为 Z> GO> B 和 GO> B> Z。在动力学方面，所有吸附剂都符合假二阶反应动力学。如果 B 和 Z 的 ΔG° 值为正值，则说明吸附属于物理吸附；如果 GO 的 ΔG° 值为负值，则说明吸附属于化学吸附。对所得到的 ΔH° 值进行研究后发现，B 和 Z 的反应是内热反应，而 GO 的反应是放热反应。B 和 Z 吸附物种的 ΔS° 值为负数，表明固体和液体之间的随机性减小，而 GO 的 ΔS° 值为正数，表明固体和液体之间的随机性增大。在最佳条件下测量得到的最高吸附容量值为：GO 589 mg g-1，B 和 Z 分别为 15.7 和 19.8 mg g-1。
{"title":"Removal of Bromophenol Blue from Aqueous Solution Using Bentonite, Zeolite and Graphene Oxide","authors":"Nuket Kartal Temel, İbrahim Gökçe Erdem","doi":"10.17776/csj.1424592","DOIUrl":"https://doi.org/10.17776/csj.1424592","url":null,"abstract":"In this study, an anionic dyestuff bromophenol blue (BrPB) from the aqueous solution was removed by adsorption in the presence of bentonite (B), zeolite (Z) and graphene oxide (GO). The effects of pH, adsorbent amount, initial concentration of BrPB, temperature and time on adsorption were investigated. After the adsorption process, ultraviolet-visible region spectrophotometer (UV-VIS) was used to measure the amount of dyestuff remaining in the solution which is not adsorbed. It’s found that; the optimum values obtained for the adsorption process; the optimum pH value 5 for B and Z, 4 for GO; 0.03 g amount of adsorbent and 60 minutes for the equilibrium time. The compatibility of Langmiur and Freundlich isotherm models was found as Z> GO> B and GO> B> Z respectively. In terms of kinetics aspect, it was observed that all adsorbents were comply with pseudo-second order reaction kinetics. To be positive of ΔG° value for B and Z is of physical nature of adsorption, to be negative of ΔG° value for GO requires to be chemical nature of adsorption. When the obtained ΔH° values were examined, it was observed that the reaction was endothermic for B and Z and exothermic for GO. The negative value of ΔS° for B and Z adsorbent species indicates that the randomness between solid and liquid decreases, whereas the positive ΔS° value for GO indicates that the randomness between solid and liquid increases. The highest adsorption capacity value obtained after the measurements made under optimum conditions is 589 mg g-1 for GO and 15.7 and 19.8 mg g-1 for B and Z, respectively.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":" 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140386703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Crocus sativus L. has been used as spice, food colouring and medicinal plant for thousands of years. In this study, antioxidant properties and cytotoxic effect of saffron extract were evaluated in HT-22 cell lines. Malignant and non-malignant cells (L929) were cultured in DMEM medium and incubated with different concentrations of saffron extract in different solvents (water, ethanol, ethyl-acetate, n-hexane). Cell viability was determined by MTT assay. Antioxidant parameters such as DPPH, FRAP, total polyphenol and flavonoid amounts were measured. The highest DPPH value was found as 15.90±0.12 µg/mL in ethyl acetate solvent. Total polyphenols were 89.06±0.02 mg GAE/g extract, flavonoids were 76.98±2.08 µg QE/g extract and FRAP was 67.93±0.98 mg/g extract in ethanol solvent. In colon cancer, IC50 value 94±1.37 µg/mL was found to be the highest value. This is thought to be due to the high antioxidant capacity in ethanol solvent. While antioxidant capacity was not found to be high, antiproliferation effect was found to be high. Saffron may also be considered as a promising chemotherapeutic agent in cancer treatment in the future.
{"title":"Crocus Officinalis (L.) Extract on Human Colerectal Cancer Cell Line (HT-22): Investigation in Vitro","authors":"S. Soylu","doi":"10.17776/csj.1419826","DOIUrl":"https://doi.org/10.17776/csj.1419826","url":null,"abstract":"Crocus sativus L. has been used as spice, food colouring and medicinal plant for thousands of years. In this study, antioxidant properties and cytotoxic effect of saffron extract were evaluated in HT-22 cell lines. Malignant and non-malignant cells (L929) were cultured in DMEM medium and incubated with different concentrations of saffron extract in different solvents (water, ethanol, ethyl-acetate, n-hexane). Cell viability was determined by MTT assay. Antioxidant parameters such as DPPH, FRAP, total polyphenol and flavonoid amounts were measured. The highest DPPH value was found as 15.90±0.12 µg/mL in ethyl acetate solvent. Total polyphenols were 89.06±0.02 mg GAE/g extract, flavonoids were 76.98±2.08 µg QE/g extract and FRAP was 67.93±0.98 mg/g extract in ethanol solvent. In colon cancer, IC50 value 94±1.37 µg/mL was found to be the highest value. This is thought to be due to the high antioxidant capacity in ethanol solvent. While antioxidant capacity was not found to be high, antiproliferation effect was found to be high. Saffron may also be considered as a promising chemotherapeutic agent in cancer treatment in the future.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":" 109","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140391817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In recent years, resistance to antimicrobials has become a global problem. Despite the need for new antibiotics with the increase of resistant bacteria, developing new antimicrobials is problematic. Biofilms formed by microorganisms play an essential role in the development of resistance. We aimed to investigate the antimicrobial and antibiofilm activities of N-heterocyclic carbene (NHC) complexes. In this study, previously synthesized and characterized NHC complexes on standard bacterial and fungal strains were investigated. The minimal inhibition concentration (MIC) test was used to determine the antimicrobial activities of the compounds, and the biofilm inhibition concentration test was used to determine the anti-biofilm activities. Compounds 2b and 2c showed potent antimicrobial activity on microorganisms between <=1.9 and 7.8µg/mL. Antimicrobial activity in salts of compounds (1a-1c) was weaker than silver compounds (2a-2c). The antibiofilm activity was between 27 and 79%, especially in silver-bound compounds (2a-2c). Benzimidazole derivative NHC compounds that we evaluated in our study were found to have significant antimicrobial and antibiofilm effects on pathogenic microorganisms. These compounds, which we assessed in our study, may be antimicrobial drug candidates that can be used in different areas. It will be essential to conduct further in vitro and in vivo studies on this subject.
{"title":"Evaluation of Antibiofilm and Antimicrobial Activities of N-heterocyclic Carbene Complexes","authors":"Uğur Tutar, Cem Çelik, M. Atas","doi":"10.17776/csj.1390256","DOIUrl":"https://doi.org/10.17776/csj.1390256","url":null,"abstract":"In recent years, resistance to antimicrobials has become a global problem. Despite the need for new antibiotics with the increase of resistant bacteria, developing new antimicrobials is problematic. Biofilms formed by microorganisms play an essential role in the development of resistance. We aimed to investigate the antimicrobial and antibiofilm activities of N-heterocyclic carbene (NHC) complexes. In this study, previously synthesized and characterized NHC complexes on standard bacterial and fungal strains were investigated. The minimal inhibition concentration (MIC) test was used to determine the antimicrobial activities of the compounds, and the biofilm inhibition concentration test was used to determine the anti-biofilm activities. Compounds 2b and 2c showed potent antimicrobial activity on microorganisms between <=1.9 and 7.8µg/mL. Antimicrobial activity in salts of compounds (1a-1c) was weaker than silver compounds (2a-2c). The antibiofilm activity was between 27 and 79%, especially in silver-bound compounds (2a-2c). Benzimidazole derivative NHC compounds that we evaluated in our study were found to have significant antimicrobial and antibiofilm effects on pathogenic microorganisms. These compounds, which we assessed in our study, may be antimicrobial drug candidates that can be used in different areas. It will be essential to conduct further in vitro and in vivo studies on this subject.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140395947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There are many fit statistics used in the structural equation modeling, and new ones are consistently being developed. Because of the variety of fit statistics, it is very important to be able to decide which fit statistics are appropriate to use in studies. When comparing any two statistics, the asymptotic relative efficiency (ARE) between them is used. The ARE can use as a power of the fit indices is one of the familiar optimal criteria. It is frequently more convenient, and also more suggestive, to use a measure of relative merit called the relative efficiency. This study aimed to compare of fit indices using Fraser’s asymptotic relative efficiency. The data sets were derived from the multivariate normal distribution using the mean vector and covariance matrix. It was determined that the most efficient fit indices in terms of asymptotic relative efficiency were Z-Test of Wilson & Hilferty (W&H), Root Mean Square Error of Approximation (RMSEA), and Chi-Square indices, respectively.
结构方程建模中使用的拟合统计量有很多，而且新的拟合统计量还在不断开发中。由于拟合统计量种类繁多，因此在研究中决定使用哪种拟合统计量是非常重要的。在比较任何两个统计量时，都要使用它们之间的渐近相对效率（ARE）。ARE 可以用作拟合指数的幂次，是我们熟悉的最优标准之一。通常，使用一种称为相对效率的相对优劣度量更为方便，也更具暗示性。本研究旨在使用 Fraser 的渐近相对效率对拟合指数进行比较。数据集来自多元正态分布，使用均值向量和协方差矩阵。结果表明，就渐近相对效率而言，最有效的拟合指数分别是 Wilson & Hilferty（W&H）的 Z 检验指数、均方根近似误差（RMSEA）指数和 Chi-Square 指数。
{"title":"Asymptotic Relative Efficiency Comparison for some Fit Indices in Structural Equation Modeling","authors":"İlkay Doğan, İ. Doğan, N. Doğan","doi":"10.17776/csj.1328880","DOIUrl":"https://doi.org/10.17776/csj.1328880","url":null,"abstract":"There are many fit statistics used in the structural equation modeling, and new ones are consistently being developed. Because of the variety of fit statistics, it is very important to be able to decide which fit statistics are appropriate to use in studies. When comparing any two statistics, the asymptotic relative efficiency (ARE) between them is used. The ARE can use as a power of the fit indices is one of the familiar optimal criteria. It is frequently more convenient, and also more suggestive, to use a measure of relative merit called the relative efficiency. This study aimed to compare of fit indices using Fraser’s asymptotic relative efficiency. The data sets were derived from the multivariate normal distribution using the mean vector and covariance matrix. It was determined that the most efficient fit indices in terms of asymptotic relative efficiency were Z-Test of Wilson & Hilferty (W&H), Root Mean Square Error of Approximation (RMSEA), and Chi-Square indices, respectively.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"108 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140394970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Caenorhabditis elegans (C. elegans), with its superiority and physiological aging properties, has become a widely recognized model system in research on aging, longevity mechanisms, age-related diseases, and drug screening. Lifespan-extending mutations in C. elegans are known to slow the aging process by interfering with a number of signaling pathways such as the AMP-activated protein kinase (AMPK), mechanistic target of rapamycin (mTOR) and insulin/IGF-1 signaling (IIS) pathways. We aimed to see how Aloe vera (A. vera) gel affects the fertilization and lifespan of C. elegans. In the presence of all A. vera gel concentrations (0.312 - 5 mg/mL), the fertilization capacity of N2 worms increased and extended their lifespan as well as increased their body size. We found that in N2 worms cultured with 2.5 mg/ml A. vera gel, sgk-1, age-1, and let-363 mRNA expression was significantly increased, while rsks-1 mRNA expression was significantly decreased. We therefore conclude that it may extend lifespan through a mechanism specifically dependent on mTOR signaling. All these observations will provide a new perspective on mammalian life extension through the model organism C. elegans.
{"title":"Aloe vera Gel Extract Prolongs Lifespan in Caenorhabditis elegans","authors":"Ş. Berk","doi":"10.17776/csj.1400264","DOIUrl":"https://doi.org/10.17776/csj.1400264","url":null,"abstract":"Caenorhabditis elegans (C. elegans), with its superiority and physiological aging properties, has become a widely recognized model system in research on aging, longevity mechanisms, age-related diseases, and drug screening. Lifespan-extending mutations in C. elegans are known to slow the aging process by interfering with a number of signaling pathways such as the AMP-activated protein kinase (AMPK), mechanistic target of rapamycin (mTOR) and insulin/IGF-1 signaling (IIS) pathways. We aimed to see how Aloe vera (A. vera) gel affects the fertilization and lifespan of C. elegans. In the presence of all A. vera gel concentrations (0.312 - 5 mg/mL), the fertilization capacity of N2 worms increased and extended their lifespan as well as increased their body size. We found that in N2 worms cultured with 2.5 mg/ml A. vera gel, sgk-1, age-1, and let-363 mRNA expression was significantly increased, while rsks-1 mRNA expression was significantly decreased. We therefore conclude that it may extend lifespan through a mechanism specifically dependent on mTOR signaling. All these observations will provide a new perspective on mammalian life extension through the model organism C. elegans.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"34 S130","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140395304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study focuses on the effect of pomegranate peel extract (PPE) as a natural medicinal substance and 45S5 bioglass (BG) particles as a bioactive material on the microstructure, antioxidant properties, and fibroblast cell cytotoxicity of biocompatible poly(ε-caprolactone) (PCL) nanofiber scaffolds. The hybrid nanofibers were fabricated via the electrospinning technique. The microstructure of nanofiber scaffolds was characterized by using scanning electron microscopy (SEM). The results indicated that the incorporation of PPE and BG particles did not change the morphology of the fibrous structure of the PCL nanofiber scaffolds. The DPPH analysis was performed to determine the antioxidant properties of nanofiber scaffolds and demonstrated that the incorporation of PPE improves the antioxidant properties of scaffolds. Cell cytotoxicity studies using fibroblast L929 cells also showed that high cell viability values were observed for hybrid PPE and BG loaded PCL nanofiber scaffolds. The findings proved that the integration of PPE and BG particles into PCL nanofibers yielded favorable characteristics suitable for wound dressing purposes, involving improved antioxidant capacity.
本研究主要探讨了天然药用物质石榴皮提取物（PPE）和生物活性材料 45S5 生物玻璃（BG）颗粒对生物相容性聚（ε-己内酯）（PCL）纳米纤维支架的微观结构、抗氧化性和成纤维细胞细胞毒性的影响。混合纳米纤维是通过电纺丝技术制成的。使用扫描电子显微镜（SEM）对纳米纤维支架的微观结构进行了表征。结果表明，PPE 和 BG 颗粒的加入并没有改变 PCL 纳米纤维支架的纤维结构形态。通过 DPPH 分析确定了纳米纤维支架的抗氧化性，结果表明加入 PPE 提高了支架的抗氧化性。使用成纤维细胞 L929 进行的细胞毒性研究也表明，混合 PPE 和 BG 负载 PCL 纳米纤维支架具有较高的细胞存活率。研究结果表明，将 PPE 和 BG 颗粒融入 PCL 纳米纤维可产生适合伤口敷料的良好特性，包括提高抗氧化能力。
{"title":"Electrospun Poly(ϵ-caprolactone) Nanofibers Containing Pomegranate Peel Extract and Bioactive Glass as Potential Wound Dressings","authors":"Ayşen Aktürk","doi":"10.17776/csj.1383556","DOIUrl":"https://doi.org/10.17776/csj.1383556","url":null,"abstract":"This study focuses on the effect of pomegranate peel extract (PPE) as a natural medicinal substance and 45S5 bioglass (BG) particles as a bioactive material on the microstructure, antioxidant properties, and fibroblast cell cytotoxicity of biocompatible poly(ε-caprolactone) (PCL) nanofiber scaffolds. The hybrid nanofibers were fabricated via the electrospinning technique. The microstructure of nanofiber scaffolds was characterized by using scanning electron microscopy (SEM). The results indicated that the incorporation of PPE and BG particles did not change the morphology of the fibrous structure of the PCL nanofiber scaffolds. The DPPH analysis was performed to determine the antioxidant properties of nanofiber scaffolds and demonstrated that the incorporation of PPE improves the antioxidant properties of scaffolds. Cell cytotoxicity studies using fibroblast L929 cells also showed that high cell viability values were observed for hybrid PPE and BG loaded PCL nanofiber scaffolds. The findings proved that the integration of PPE and BG particles into PCL nanofibers yielded favorable characteristics suitable for wound dressing purposes, involving improved antioxidant capacity.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"39 S181","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140395068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the synthesis of 2-(2-acetyl-1H-benzimidazol-1-yl)-1-arylethanone (3a-3d) and 1-methyl-3-phenyl-benzo[4,5]imidazo[1,2-a]pyrazine derivatives (4a-4d) and to investigate their cytotoxic activity were aimed. APCI, IR, 1HNMR, and 13CNMR spectra were utilized to determine the structure of the synthesized compounds. The cytotoxic activity of selected compounds were detected in A549 (human lung carcinoma) and NIH3T3 (mouse embryonic fibroblasts) cell lines. Compounds 4c and 4d were found to be selectively cytotoxic against A549 and NIH3T3 cell lines. Molecular docking studies were performed using the data retrieved from the Protein Data Bank server (PDBID: 4QTX).
{"title":"Synthesis, Cytotoxic Activity Evaluation and Molecular Docking Studies of Some Benzimidazole Derivatives","authors":"Aybüke Züleyha Kaya, Derya Osmaniye, Asaf Evrim Evren, Leyla Yurttaş, Ş. Demirayak","doi":"10.17776/csj.1392037","DOIUrl":"https://doi.org/10.17776/csj.1392037","url":null,"abstract":"In this study, the synthesis of 2-(2-acetyl-1H-benzimidazol-1-yl)-1-arylethanone (3a-3d) and 1-methyl-3-phenyl-benzo[4,5]imidazo[1,2-a]pyrazine derivatives (4a-4d) and to investigate their cytotoxic activity were aimed. APCI, IR, 1HNMR, and 13CNMR spectra were utilized to determine the structure of the synthesized compounds. The cytotoxic activity of selected compounds were detected in A549 (human lung carcinoma) and NIH3T3 (mouse embryonic fibroblasts) cell lines. Compounds 4c and 4d were found to be selectively cytotoxic against A549 and NIH3T3 cell lines. Molecular docking studies were performed using the data retrieved from the Protein Data Bank server (PDBID: 4QTX).","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"86 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140395391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
İdil Karaca Açarı, Duygu Özhan Turhan, Ali Kuruçay, Süleyman Köytepe, B. Ateş
In this study, gum arabic based network polymers were prepared using epoxy functional PEG structures. The basic physicochemical properties of these structures, their structural characterization, thermal properties and morphological properties were investigated. Toxicity properties of constructs synthesized on zebrafish (Danio rerio (Hamilton)) offspring were determined in vivo. In addition, in vitro toxicity tests were performed on L929 fibroblast cells. When the general properties of these structures were examined. Structural and thermal properties were better with increasing cross-linker rates ratios (1%, 3%, 5%). According to the toxicity test performed on zebrafish juveniles; GA-PEG-Epox (1%) constructs are non-toxic to zebrafish juveniles. The mortality rate of GA-PEG-Epox (3%) and GA-PEG-Epox (5%) structures was observed as 12.5% and 20.8%, respectively. It was observed that the structures were not toxic to zebrafish juveniles. MTT test performed on L929 fibroblast cells, high cell viability (>90%) was observed in all synthesized structures. These results are evaluated as Grade 1 according to ISO standards.
{"title":"Synthesis of Gum Arabic-Based Biopolymer Network and Determination of Its Toxicity Properties in In Vitro - In Vivo Model Systems","authors":"İdil Karaca Açarı, Duygu Özhan Turhan, Ali Kuruçay, Süleyman Köytepe, B. Ateş","doi":"10.17776/csj.1385443","DOIUrl":"https://doi.org/10.17776/csj.1385443","url":null,"abstract":"In this study, gum arabic based network polymers were prepared using epoxy functional PEG structures. The basic physicochemical properties of these structures, their structural characterization, thermal properties and morphological properties were investigated. Toxicity properties of constructs synthesized on zebrafish (Danio rerio (Hamilton)) offspring were determined in vivo. In addition, in vitro toxicity tests were performed on L929 fibroblast cells. When the general properties of these structures were examined. Structural and thermal properties were better with increasing cross-linker rates ratios (1%, 3%, 5%). According to the toxicity test performed on zebrafish juveniles; GA-PEG-Epox (1%) constructs are non-toxic to zebrafish juveniles. The mortality rate of GA-PEG-Epox (3%) and GA-PEG-Epox (5%) structures was observed as 12.5% and 20.8%, respectively. It was observed that the structures were not toxic to zebrafish juveniles. MTT test performed on L929 fibroblast cells, high cell viability (>90%) was observed in all synthesized structures. These results are evaluated as Grade 1 according to ISO standards.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"23 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140396555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this paper, we study the magnetic curves on a Kähler manifold which is conformally equivalent to Euclidean Schwarzschild space. We show that Euclidean Schwarzschild space is locally conformally Kähler and transform it into a Kähler space by applying a conformal factor coming from its Lee form. We solve Lorentz equation to find analytical expressions for magnetic curves which are compatible with the almost complex structure of the proposed Kähler manifold. We also calculate the energy of magnetic curves.
{"title":"Kähler Magnetic Curves in Conformally Euclidean Schwarzschild Space","authors":"Özgür Kelekçi","doi":"10.17776/csj.1400543","DOIUrl":"https://doi.org/10.17776/csj.1400543","url":null,"abstract":"In this paper, we study the magnetic curves on a Kähler manifold which is conformally equivalent to Euclidean Schwarzschild space. We show that Euclidean Schwarzschild space is locally conformally Kähler and transform it into a Kähler space by applying a conformal factor coming from its Lee form. We solve Lorentz equation to find analytical expressions for magnetic curves which are compatible with the almost complex structure of the proposed Kähler manifold. We also calculate the energy of magnetic curves.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"26 1‐2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140397906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Çiğdem Sevim, Sabri Ünal, Temel Kan Bakır, Mertcan Karadeniz, Ali Taghizadeh Tgzd
Colorectal cancer ranks as the third most prevalent form of cancer and stands as the second leading cause of mortality. Both environmental and genetic risk factors contribute to its manifestation. Presently, 5-fluoruracil/leucovorin (5-FU/LV) remains the recommended course for adjuvant therapy in addressing this condition. Conversely, mushrooms, celebrated for their biologically active constituents, including valuable enzymes, have emerged as a captivating subject in diverse medical disciplines, particularly within the realm of cancer therapy, due to their promising therapeutic properties. This specific investigation aimed to conduct in vitro cytotoxic experiments using extracts obtained from Heterobasidion annosum micelles cultivated in a liquid malt extract medium. The pulverized extracts were dissolved in Dulbecco's Modified Eagle Medium (DMEM) at varied concentrations ranging from 25ng/mL to 200ng/mL and subsequently administered to colon adenocarcinoma (Caco-2) cells. The cytotoxic effects of both the fungus and cisplatin, a well-known anticarcinogenic agent, were examined at intervals of 24, 48, and 72 hours. The findings indicated a significant inhibition of cancer cell development within this timeframe. Moreover, a noteworthy discovery emerged, revealing that cisplatin, known for its efficacy in various cancer studies, substantially diminished the viability of cancer cells after 72 hours in comparison to the control group.
{"title":"Cytotoxicity Analysis of the Effects of Heterobasidion Annosum Mycelia and Cisplatin on Colon Adenocarcinoma (CACO-2) Cell Line","authors":"Çiğdem Sevim, Sabri Ünal, Temel Kan Bakır, Mertcan Karadeniz, Ali Taghizadeh Tgzd","doi":"10.17776/csj.1357215","DOIUrl":"https://doi.org/10.17776/csj.1357215","url":null,"abstract":"Colorectal cancer ranks as the third most prevalent form of cancer and stands as the second leading cause of mortality. Both environmental and genetic risk factors contribute to its manifestation. Presently, 5-fluoruracil/leucovorin (5-FU/LV) remains the recommended course for adjuvant therapy in addressing this condition. Conversely, mushrooms, celebrated for their biologically active constituents, including valuable enzymes, have emerged as a captivating subject in diverse medical disciplines, particularly within the realm of cancer therapy, due to their promising therapeutic properties. This specific investigation aimed to conduct in vitro cytotoxic experiments using extracts obtained from Heterobasidion annosum micelles cultivated in a liquid malt extract medium. The pulverized extracts were dissolved in Dulbecco's Modified Eagle Medium (DMEM) at varied concentrations ranging from 25ng/mL to 200ng/mL and subsequently administered to colon adenocarcinoma (Caco-2) cells. The cytotoxic effects of both the fungus and cisplatin, a well-known anticarcinogenic agent, were examined at intervals of 24, 48, and 72 hours. The findings indicated a significant inhibition of cancer cell development within this timeframe. Moreover, a noteworthy discovery emerged, revealing that cisplatin, known for its efficacy in various cancer studies, substantially diminished the viability of cancer cells after 72 hours in comparison to the control group.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"7 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140426520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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