H. I. Ulusoy, İpek Nur Yi̇ği̇t, Ümmügülsüm Polat, Esra Durgun, Aslıhan Gürbüzer, Songül Ulusoy
An enrichment and determination method based on liquid chromatographically analysis and cloud point extraction (CPE) has been developed for trace levels of B vitamins (B1, B9 and B12) in the proposed study. Vitamin molecules were drawn into the non-ionic surfactant phase of Polyethylene Glycol (PEG-6000) in the presence of pH 9.0 medium. The surfactant-rich phase separated by centrifugation and then dissolved with 700 µL of ethanol. The obtained ethanol phase was filtered by 0.45-micron filter prior to the HPLC analysis. All parameters affecting the CPE method such as pH, buffer volume, incubation time, surfactant and electrolyte concentration, solvent for the surfactant-rich phase and its amount have been individually studied and optimized step by step. After the optimization of all parameters of the CPE process, the detection limits of the developed method for B1, B9 and B12 vitamins were calculated as 1.42 ng mL-1, 7.14 ng mL-1 and 14.28 ng mL-1 , respectively. The linear working ranges for three vitamin molecules was obtained in the range of 5.0-500.0 ng mL-1. After CPE procedure, determination of vitamin molecules was carried out by using HPLC system with diode array detector(DAD) at 244 nm for vitamin B1, 285 nm for vitamin B9, and 361 nm for vitamin B12, respectively
{"title":"Simultaneously HPLC Analysis of B1, B9 and B12 Vitamins at Trace Levels via Cloud Point Extraction","authors":"H. I. Ulusoy, İpek Nur Yi̇ği̇t, Ümmügülsüm Polat, Esra Durgun, Aslıhan Gürbüzer, Songül Ulusoy","doi":"10.17776/csj.1342960","DOIUrl":"https://doi.org/10.17776/csj.1342960","url":null,"abstract":"An enrichment and determination method based on liquid chromatographically analysis and cloud point extraction (CPE) has been developed for trace levels of B vitamins (B1, B9 and B12) in the proposed study. Vitamin molecules were drawn into the non-ionic surfactant phase of Polyethylene Glycol (PEG-6000) in the presence of pH 9.0 medium. The surfactant-rich phase separated by centrifugation and then dissolved with 700 µL of ethanol. The obtained ethanol phase was filtered by 0.45-micron filter prior to the HPLC analysis. All parameters affecting the CPE method such as pH, buffer volume, incubation time, surfactant and electrolyte concentration, solvent for the surfactant-rich phase and its amount have been individually studied and optimized step by step. After the optimization of all parameters of the CPE process, the detection limits of the developed method for B1, B9 and B12 vitamins were calculated as 1.42 ng mL-1, 7.14 ng mL-1 and 14.28 ng mL-1 , respectively. The linear working ranges for three vitamin molecules was obtained in the range of 5.0-500.0 ng mL-1. After CPE procedure, determination of vitamin molecules was carried out by using HPLC system with diode array detector(DAD) at 244 nm for vitamin B1, 285 nm for vitamin B9, and 361 nm for vitamin B12, respectively","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"47 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139151319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Prostate cancer ranks as the second most prevalent cancer in men globally. One of the evolving subjects of investigation in prostate cancer is the role of N6-methyladenosine (m6A) modifications. Hydroxychloroquine (HCQ), an autophagy inhibitor, was shown to be promising in enhancing the response to chemotherapy in prostate cancer. The interplay between autophagy and m6A is an emerging area of research. However, the relationship between m6A modifications and HCQ remains unclear. The objective of this study was to examine the effect of HCQ on the regulation of m6A methylation in prostate cancer. Initially, the cytotoxic effect of HCQ on LNCaP and PC3 cells was evaluated. The IC50 values for each cell were calculated. Finally, m6A levels in HCQ-treated and untreated cells were determined using m6A RNA methylation quantification kit. HCQ showed a significant dose- and time-dependent reduction in cell viability. Following HCQ treatment, a statistically significant decrease in m6A levels was observed: from 0.050±0.001% to 0.013±0.02% in PC3 cells and from 0.039±0.001% to 0.016±0.01% in LNCaP cells. The study unveils for the first time that HCQ affects m6A methylation in prostate cancer. The impact of autophagy inhibitor HCQ on m6A modifications introduces a novel dimension to its potential mechanisms of action.
{"title":"Hidroksiklorokin Prostat Kanseri Hücrelerinde m6A RNA Metilasyonunu Düzenler","authors":"Sevinc Yanar, Merve Gülsen BAL ALBAYRAK","doi":"10.17776/csj.1307100","DOIUrl":"https://doi.org/10.17776/csj.1307100","url":null,"abstract":"Prostate cancer ranks as the second most prevalent cancer in men globally. One of the evolving subjects of investigation in prostate cancer is the role of N6-methyladenosine (m6A) modifications. Hydroxychloroquine (HCQ), an autophagy inhibitor, was shown to be promising in enhancing the response to chemotherapy in prostate cancer. The interplay between autophagy and m6A is an emerging area of research. However, the relationship between m6A modifications and HCQ remains unclear. The objective of this study was to examine the effect of HCQ on the regulation of m6A methylation in prostate cancer. Initially, the cytotoxic effect of HCQ on LNCaP and PC3 cells was evaluated. The IC50 values for each cell were calculated. Finally, m6A levels in HCQ-treated and untreated cells were determined using m6A RNA methylation quantification kit. HCQ showed a significant dose- and time-dependent reduction in cell viability. Following HCQ treatment, a statistically significant decrease in m6A levels was observed: from 0.050±0.001% to 0.013±0.02% in PC3 cells and from 0.039±0.001% to 0.016±0.01% in LNCaP cells. The study unveils for the first time that HCQ affects m6A methylation in prostate cancer. The impact of autophagy inhibitor HCQ on m6A modifications introduces a novel dimension to its potential mechanisms of action.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"48 41","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139151155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tülin GÜRKAN POLAT, Ahmet Gençer, Meltem Asi̇ltürk, Yılmaz Aksu
In this study microcapsules were prepared by in-situ polymerization route with melamine formaldehyde as a shell material and tung oil as core material. Melamine formaldehyde (MF), a thermosetting polymer, is one of the most widely used monomers in microencapsulation due to its superior mechanical strength and thermal stability. Tung oil contains unsaturated double bonds that can be oxidized to form a film in air. Tung oil is fast drying and biodegradable, besides it is low cost and does not pollute the environment. Most importantly, tung oil is a versatile substance in industry. Therefore, tung oil is a good choice as core material. The chemical structure of microcapsules were characterized by Fourier Transform Infrared (FTIR) spectroscopy. The surface morphology and particle size and distribution were evaluated by Scanning Electron Microscopy (SEM). The thermal behavior of microcapsules and tung oil were studied by thermogravimetric analysis (TGA). The results showed that the spherical microcapsules (particle size of mostly 4-5 μm) were produced with a filling content of 15.64 wt.%, and a yield of 49.78 wt.%. The microcapsules exhibit a good thermal stability
{"title":"Preparation and Characterization of Tung Oil Loaded Melamine Formaldehyde Microcapsules","authors":"Tülin GÜRKAN POLAT, Ahmet Gençer, Meltem Asi̇ltürk, Yılmaz Aksu","doi":"10.17776/csj.1299101","DOIUrl":"https://doi.org/10.17776/csj.1299101","url":null,"abstract":"In this study microcapsules were prepared by in-situ polymerization route with melamine formaldehyde as a shell material and tung oil as core material. Melamine formaldehyde (MF), a thermosetting polymer, is one of the most widely used monomers in microencapsulation due to its superior mechanical strength and thermal stability. Tung oil contains unsaturated double bonds that can be oxidized to form a film in air. Tung oil is fast drying and biodegradable, besides it is low cost and does not pollute the environment. Most importantly, tung oil is a versatile substance in industry. Therefore, tung oil is a good choice as core material. The chemical structure of microcapsules were characterized by Fourier Transform Infrared (FTIR) spectroscopy. The surface morphology and particle size and distribution were evaluated by Scanning Electron Microscopy (SEM). The thermal behavior of microcapsules and tung oil were studied by thermogravimetric analysis (TGA). The results showed that the spherical microcapsules (particle size of mostly 4-5 μm) were produced with a filling content of 15.64 wt.%, and a yield of 49.78 wt.%. The microcapsules exhibit a good thermal stability","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"276 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139152655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zekeriya Keski̇n, Fatih Yulak, Hatice Terzi̇, M. İnanir
There are many challenges in the treatment of Burkitt lymphoma, especially in immunocompromised individuals, elderly patients, and patients with relapsed or refractory disease. Therefore, there is a need for new and less toxic therapeutic agents. The aim of this study was to determine the antitumoral activity of omipalisib, a PI3K/AKT/mTOR pathway inhibitor, in the Burkitt lymphoma. Raji cell line was used in the study. Omipalisib was administered to the cell line and then the cytotoxic effect of omipalisib on Raji cells was evaluated by the XTT test. The IC50 value was calculated according to the results of the XTT test. Apoptosis and cell cycle experiments were studied with the calculated IC50 value. The flow cytometric method was used to determine the effect of omipalisib on apoptosis and cell death. The results of the study showed a statistically significant cytotoxic effect of increasing concentrations of omipalisib on Raji cells. The apoptosis experiment performed revealed that omipalisib strongly induced apoptosis. The cell cycle experiment showed that omipalisib stimulated the cell cycle arrest at the G0/G1 phase. It was concluded that omipalisib exhibited antitumoral activity on Burkitt lymphoma cells with its cytotoxic effect and induced apoptosis and cell cycle arrest. Considering this effect, targeting the PI3K/AKT/mTOR pathway with omipalisib can be a new treatment option.
{"title":"Evaluation of the Antitumor Activity of Omipalisib, a PI3K/AKT/MTOR Pathway Inhibitor, on Burkitt Lymphoma Cell Line","authors":"Zekeriya Keski̇n, Fatih Yulak, Hatice Terzi̇, M. İnanir","doi":"10.17776/csj.1344535","DOIUrl":"https://doi.org/10.17776/csj.1344535","url":null,"abstract":"There are many challenges in the treatment of Burkitt lymphoma, especially in immunocompromised individuals, elderly patients, and patients with relapsed or refractory disease. Therefore, there is a need for new and less toxic therapeutic agents. The aim of this study was to determine the antitumoral activity of omipalisib, a PI3K/AKT/mTOR pathway inhibitor, in the Burkitt lymphoma. Raji cell line was used in the study. Omipalisib was administered to the cell line and then the cytotoxic effect of omipalisib on Raji cells was evaluated by the XTT test. The IC50 value was calculated according to the results of the XTT test. Apoptosis and cell cycle experiments were studied with the calculated IC50 value. The flow cytometric method was used to determine the effect of omipalisib on apoptosis and cell death. The results of the study showed a statistically significant cytotoxic effect of increasing concentrations of omipalisib on Raji cells. The apoptosis experiment performed revealed that omipalisib strongly induced apoptosis. The cell cycle experiment showed that omipalisib stimulated the cell cycle arrest at the G0/G1 phase. It was concluded that omipalisib exhibited antitumoral activity on Burkitt lymphoma cells with its cytotoxic effect and induced apoptosis and cell cycle arrest. Considering this effect, targeting the PI3K/AKT/mTOR pathway with omipalisib can be a new treatment option.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"266 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139152829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, a novel triazolopyrimidinone derivative, 2-(2-chlorophenyl)-5-(morpholinomethyl)-[1,2,4]triazolo[1,5-a]pyrimidin-7(3H)-one, abbreviated as CPD-1, was synthesized as a drug candidate. By employing electrochemical techniques, we analyzed the electrochemical behavior of this compound and its interactions with both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Experimental parameters such as pH, concentration, scan rate, immobilization time were studied using Differential Pulse Voltammetry (DPV) and Cyclic Voltammetry (CV) to obtain the most precise analytical signals. We present an innovative approach to evaluate the toxicity effect of this drug candidate on DNA. We also propose a simplified equation to quantify toxicity effects based on changes in electrochemical signals, specifically peak current of guanine bases, before and after drug-DNA interactions. Our methodology contributes to the burgeoning field of electrochemical toxicity assessment and holds promise for advancing drug development and safety evaluation. Furthermore, stability tests for the drug candidate were conducted on different days. Notably, our investigation revealed significant alterations in guanine bases upon the interaction of CPD-1 with both ssDNA and dsDNA, underscoring the potential impact of these compounds on DNA structure. Based on our experimental data, we conclude that this molecule can be utilized as a drug due to its effects on DNA.
本研究合成了一种新型三唑嘧啶酮衍生物--2-(2-氯苯基)-5-(吗啉甲基)-[1,2,4]三唑并[1,5-a]嘧啶-7(3H)-酮，简称 CPD-1，作为候选药物。我们采用电化学技术分析了该化合物的电化学行为及其与单链 DNA（ssDNA）和双链 DNA（dsDNA）的相互作用。我们使用差分脉冲伏安法（DPV）和循环伏安法（CV）研究了 pH 值、浓度、扫描速率、固定时间等实验参数，以获得最精确的分析信号。我们提出了一种创新方法来评估这种候选药物对 DNA 的毒性影响。我们还提出了一个简化方程，根据药物与 DNA 相互作用前后电化学信号（特别是鸟嘌呤碱基的峰值电流）的变化来量化毒性效应。我们的方法为新兴的电化学毒性评估领域做出了贡献，并有望推动药物开发和安全性评估。此外，我们还在不同的日期对候选药物进行了稳定性测试。值得注意的是，我们的研究发现，CPD-1 与 ssDNA 和 dsDNA 相互作用时，鸟嘌呤碱基会发生显著变化，这突出表明了这些化合物对 DNA 结构的潜在影响。根据我们的实验数据，我们得出结论：由于 CPD-1 对 DNA 有影响，因此可以将其用作药物。
{"title":"A Novel Triazolopyrimidinone Derivative: A Portable Electrochemical Approach to Investigate DNA Interactions","authors":"Arif Engin Çeti̇n","doi":"10.17776/csj.1344756","DOIUrl":"https://doi.org/10.17776/csj.1344756","url":null,"abstract":"In this study, a novel triazolopyrimidinone derivative, 2-(2-chlorophenyl)-5-(morpholinomethyl)-[1,2,4]triazolo[1,5-a]pyrimidin-7(3H)-one, abbreviated as CPD-1, was synthesized as a drug candidate. By employing electrochemical techniques, we analyzed the electrochemical behavior of this compound and its interactions with both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Experimental parameters such as pH, concentration, scan rate, immobilization time were studied using Differential Pulse Voltammetry (DPV) and Cyclic Voltammetry (CV) to obtain the most precise analytical signals. We present an innovative approach to evaluate the toxicity effect of this drug candidate on DNA. We also propose a simplified equation to quantify toxicity effects based on changes in electrochemical signals, specifically peak current of guanine bases, before and after drug-DNA interactions. Our methodology contributes to the burgeoning field of electrochemical toxicity assessment and holds promise for advancing drug development and safety evaluation. Furthermore, stability tests for the drug candidate were conducted on different days. Notably, our investigation revealed significant alterations in guanine bases upon the interaction of CPD-1 with both ssDNA and dsDNA, underscoring the potential impact of these compounds on DNA structure. Based on our experimental data, we conclude that this molecule can be utilized as a drug due to its effects on DNA.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"224 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139153026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Energy crisis is the most popular issue in the world, which must be overcome with the development of alternative energy sources. Among them, methanol is a promising fuel when used in direct methanol fuel cells. However, the mentioned cell needs highly electroactive and stable anode materials toward MeOH. Ni has gained attention as it is an alternative to noble atoms. In this study, Ni was deposited on reduced graphene layer which functionalized with S atoms via the hydrothermal method. The fabricated sample was characterized by using Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), mapping, X-ray Powder Diffraction (XRD), and Brunauer–Emmett–Teller method (BET). Two samples as called Ni/GCE and Ni@s-rGO/GCE were examined for methanol oxidation reaction in alkaline media. For methanol oxidation, due to the higher surface area, and small particle size of Ni, the mass activity of Ni@s-rGO/GCE is two times higher than Ni/GCE
能源危机是世界上最受关注的问题，必须通过开发替代能源加以解决。其中，在直接甲醇燃料电池中使用甲醇是一种很有前途的燃料。然而，上述电池需要对甲醇具有高度电活性和稳定性的阳极材料。镍作为惰性原子的一种替代材料，已经引起了人们的关注。在这项研究中，通过水热法将镍沉积在用 S 原子官能化的还原石墨烯层上。使用扫描电子显微镜 (SEM)、能量色散 X 射线光谱 (EDX)、绘图、X 射线粉末衍射 (XRD) 和布鲁瑙尔-艾美特-泰勒法 (BET) 对制备的样品进行了表征。研究人员对 Ni/GCE 和 Ni@s-rGO/GCE 这两种样品在碱性介质中的甲醇氧化反应进行了检测。在甲醇氧化反应中，由于 Ni 的比表面积较大且粒径较小，Ni@s-rGO/GCE 的质量活性是 Ni/GCE 的两倍。
{"title":"Efficient Methanol Electro-oxidation on Ni, S Dual Doped Reduced Graphene Layer Catalyst","authors":"Rukan Suna, Sedef Kaplan","doi":"10.17776/csj.1278386","DOIUrl":"https://doi.org/10.17776/csj.1278386","url":null,"abstract":"Energy crisis is the most popular issue in the world, which must be overcome with the development of alternative energy sources. Among them, methanol is a promising fuel when used in direct methanol fuel cells. However, the mentioned cell needs highly electroactive and stable anode materials toward MeOH. Ni has gained attention as it is an alternative to noble atoms. In this study, Ni was deposited on reduced graphene layer which functionalized with S atoms via the hydrothermal method. The fabricated sample was characterized by using Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), mapping, X-ray Powder Diffraction (XRD), and Brunauer–Emmett–Teller method (BET). Two samples as called Ni/GCE and Ni@s-rGO/GCE were examined for methanol oxidation reaction in alkaline media. For methanol oxidation, due to the higher surface area, and small particle size of Ni, the mass activity of Ni@s-rGO/GCE is two times higher than Ni/GCE","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"25 42","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139148447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Copper oxide nanoparticles (CuO NPs) were produced by green synthesis method which is a cheap, easy and effective method using Tragopogon porrifolius extract. The shape, bond and crystal structure of the nanoparticles were determined by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and X-ray diffractometer (XRD) analysis methods. SEM analysis showed that the particles were spherical and EDX analysis showed the elemental composition of Cu and O as Cu 58.17 % and O 32.73 %. Cu-O bond structure was identified in FTIR analysis. In XRD analysis, peaks defining CuO NPs were observed. The antioxidant and photocatalytic activity of the synthesized CuO NPs were investigated. Antioxidant capacities were examined in the range of 50-500 μg/mL. The free radical scavenging activity of the nanoparticles was determined as 70.75 % at a concentration of 500 μg/mL. In photocatalytic studies, Reactive Red 120 (RR 120) dye degradation was investigated. The degradation time was calculated as 76 % in 30 min.
利用猪笼草提取物，采用廉价、简便、有效的绿色合成方法制备了氧化铜纳米粒子（CuO NPs）。通过傅立叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、能量色散 X 射线（EDX）和 X 射线衍射仪（XRD）分析方法测定了纳米颗粒的形状、结合力和晶体结构。扫描电子显微镜分析表明颗粒呈球形，能量色散 X 射线分析表明 Cu 和 O 的元素组成为 Cu 58.17 % 和 O 32.73 %。傅立叶变换红外光谱分析确定了 Cu-O 键结构。在 XRD 分析中，观察到了定义 CuO NPs 的峰值。研究了合成的 CuO NPs 的抗氧化性和光催化活性。抗氧化能力的检测范围为 50-500 μg/mL。在浓度为 500 μg/mL 时，纳米粒子的自由基清除活性为 70.75%。在光催化研究中，研究了活性红 120（RR 120）染料的降解。经计算，30 分钟内的降解率为 76%。
{"title":"Green Synthesis of CuO Nanoparticles Using Tragopogon porrifolius and Their Antioxidant and Photocatalytic Applications","authors":"Gamze TOPAL CANBAZ","doi":"10.17776/csj.1329389","DOIUrl":"https://doi.org/10.17776/csj.1329389","url":null,"abstract":"Copper oxide nanoparticles (CuO NPs) were produced by green synthesis method which is a cheap, easy and effective method using Tragopogon porrifolius extract. The shape, bond and crystal structure of the nanoparticles were determined by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) and X-ray diffractometer (XRD) analysis methods. SEM analysis showed that the particles were spherical and EDX analysis showed the elemental composition of Cu and O as Cu 58.17 % and O 32.73 %. Cu-O bond structure was identified in FTIR analysis. In XRD analysis, peaks defining CuO NPs were observed. The antioxidant and photocatalytic activity of the synthesized CuO NPs were investigated. Antioxidant capacities were examined in the range of 50-500 μg/mL. The free radical scavenging activity of the nanoparticles was determined as 70.75 % at a concentration of 500 μg/mL. In photocatalytic studies, Reactive Red 120 (RR 120) dye degradation was investigated. The degradation time was calculated as 76 % in 30 min.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"361 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139149129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Fandaklı, Tayyibe Beyza Yücel, Elif Öztürk, U. Uzuner, Burak Barut, Fatih Mehmet Ateş, N. Yaylı
A number of new methoxy-substituted 4,6-diaryl-3,4-dihydropyrimidine-2(1H)-thiones (DH-Pyr-S, 17-28) were designed and synthesized by the reaction of methoxy-substituted chalcones (1–14) with thiourea using solid-phase microwave method (MW) in view of the structural requirements as suggested in the pharmacophore model for tyrosinase inhibition (TI). Synthesized compounds were assessed for their in vitro TI potential and compounds 16, 17, and 21 exhibited notable tyrosinase inhibitory properties at the concentrations of 31.86 ± 2.45 µM, 44.58 ± 0.46 µM, and 48.47 ± 0.66 µM, respectively. Compounds (16, 17, and 21) were exhibited experimentally more potent TI than the standard used in terms of the IC50 value (Kojic acid, 55.38 ± 2.30 µM; p<0.0001). Additionally, DPPH activity of 15-28 were evaluated and compound 17 showed the moderate DPPH activity (45.64 ± 0.34%). Binding affinities of synthesized molecules to the tyrosinase catalytic core were further investigated through in silico molecular docking studies using AutoDock Vina (version 1.2.5), discovery studio accelyrs (BIOVIA, Dassault Systèmes) and predicting small-molecule pharmacokinetic properties using graph-based signatures (pkCSM) programs were used for ADMET calculations. Among synthesized compounds 15, 21, and 24 revealed high binding affinity to tyrosinase active site with lowest binding free energy (ΔG) values of -7.9 kcal/mol, thereby outperformed kojic acid affinity. In conclusion most modeling results were in agreement with their experimental data, suggesting the TI potential of lead compounds.
{"title":"MW Assisted Synthesis of New 4,6-diaryl-3,4-Didhydropyrimidines-2(1H)-thione Derivatives: Tyrosinase Inhibition, Antioxidant, and Molecular Docking Studies","authors":"S. Fandaklı, Tayyibe Beyza Yücel, Elif Öztürk, U. Uzuner, Burak Barut, Fatih Mehmet Ateş, N. Yaylı","doi":"10.17776/csj.1299843","DOIUrl":"https://doi.org/10.17776/csj.1299843","url":null,"abstract":"A number of new methoxy-substituted 4,6-diaryl-3,4-dihydropyrimidine-2(1H)-thiones (DH-Pyr-S, 17-28) were designed and synthesized by the reaction of methoxy-substituted chalcones (1–14) with thiourea using solid-phase microwave method (MW) in view of the structural requirements as suggested in the pharmacophore model for tyrosinase inhibition (TI). Synthesized compounds were assessed for their in vitro TI potential and compounds 16, 17, and 21 exhibited notable tyrosinase inhibitory properties at the concentrations of 31.86 ± 2.45 µM, 44.58 ± 0.46 µM, and 48.47 ± 0.66 µM, respectively. Compounds (16, 17, and 21) were exhibited experimentally more potent TI than the standard used in terms of the IC50 value (Kojic acid, 55.38 ± 2.30 µM; p<0.0001). Additionally, DPPH activity of 15-28 were evaluated and compound 17 showed the moderate DPPH activity (45.64 ± 0.34%). Binding affinities of synthesized molecules to the tyrosinase catalytic core were further investigated through in silico molecular docking studies using AutoDock Vina (version 1.2.5), discovery studio accelyrs (BIOVIA, Dassault Systèmes) and predicting small-molecule pharmacokinetic properties using graph-based signatures (pkCSM) programs were used for ADMET calculations. Among synthesized compounds 15, 21, and 24 revealed high binding affinity to tyrosinase active site with lowest binding free energy (ΔG) values of -7.9 kcal/mol, thereby outperformed kojic acid affinity. In conclusion most modeling results were in agreement with their experimental data, suggesting the TI potential of lead compounds.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"15 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139151654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Previous studies have reported the anticancer properties of β-glucan on various cancer cells. The objective of this research was to investigate the involvement of apoptosis in the cytotoxic action of β-glucan on the A549 cells. The cytotoxic impact of this drug on A549 cells was examined by subjecting them to various quantities of the substance, and the XTT assay was utilized to determine cell survival. Flow cytometry was performed to investigate apoptosis. A statistically significant and dose-dependent cytotoxic impact on A549 cells was observed upon treatment with β-glucan. The calculated IC50 value of β-glucan for A549 cells after a 24-hour treatment period was discovered to be 82.16 μg/mL. Further investigations carried out using the IC50 dose of β-glucan revealed a significant increase in the late apoptotic cells percentage. The capacity of β-glucan to trigger apoptosis is thought to be the cause of its cytotoxic action on lung cancer. The revelation of this discovery emphasizes the promising possibilities of β-glucan as an effective therapeutic choice.
{"title":"Unraveling the Role of Apoptosis in the Antiproliferative Activity of β-Glucan on A549 Cells","authors":"Ziad Joha, M. Ergül","doi":"10.17776/csj.1336167","DOIUrl":"https://doi.org/10.17776/csj.1336167","url":null,"abstract":"Previous studies have reported the anticancer properties of β-glucan on various cancer cells. The objective of this research was to investigate the involvement of apoptosis in the cytotoxic action of β-glucan on the A549 cells. The cytotoxic impact of this drug on A549 cells was examined by subjecting them to various quantities of the substance, and the XTT assay was utilized to determine cell survival. Flow cytometry was performed to investigate apoptosis. A statistically significant and dose-dependent cytotoxic impact on A549 cells was observed upon treatment with β-glucan. The calculated IC50 value of β-glucan for A549 cells after a 24-hour treatment period was discovered to be 82.16 μg/mL. Further investigations carried out using the IC50 dose of β-glucan revealed a significant increase in the late apoptotic cells percentage. The capacity of β-glucan to trigger apoptosis is thought to be the cause of its cytotoxic action on lung cancer. The revelation of this discovery emphasizes the promising possibilities of β-glucan as an effective therapeutic choice.","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"247 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139152708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oxidative stress is defined as an imbalance between the generation of reactive oxygen species (ROS) and their scavenging. Indatralin, which has serotonin reuptake inhibitory activity, has not yet been studied for its ability to prevent oxidative damage. Our research's objective was to find out how indatraline defends against oxidative damage. C6 cells were used in the study and four different cell groups were created. The control group received no therapy at all. For 24 hours, cells in the H2O2 group were exposed to 0.5 mM H2O2. The indatraline group received indatraline treatments for 24 hours at various doses (0.5, 1, 2.5, 5 and 10 μM). For one hour, indatraline was administered to the indatraline + H2O2 group at various concentrations (0.5, 1, 2.5, 5 and 10 μM) before the group was subjected to 0.5 mM H2O2 for 24 hours. Following the occurrence of oxidative damage, total antioxidant status (TAS) and total oxidant status (TOS) levels were determined. Cell viability was also evaluated using the XTT assay. As a result, after hydrogen peroxide-induced oxidative damage, indatraline at doses of 10, 5, and 2.5 μM showed a protective effect by significantly enhanced cell survival in C6 cells(p < 0.001). Additionally, indatraline boosted the lowered TAS level while decreasing the elevated TOS levels following hydrogen peroxide-induced oxidative damage (p<0.001).
{"title":"Investigation of the Effect of Indatraline on Oxidative Damage Induced by Hydrogen Peroxide in C6 Glioma Cell Line","authors":"Fatih Yulak, Bünyamin Üngür","doi":"10.17776/csj.1340869","DOIUrl":"https://doi.org/10.17776/csj.1340869","url":null,"abstract":"Oxidative stress is defined as an imbalance between the generation of reactive oxygen species (ROS) and their scavenging. Indatralin, which has serotonin reuptake inhibitory activity, has not yet been studied for its ability to prevent oxidative damage. Our research's objective was to find out how indatraline defends against oxidative damage. C6 cells were used in the study and four different cell groups were created. The control group received no therapy at all. For 24 hours, cells in the H2O2 group were exposed to 0.5 mM H2O2. The indatraline group received indatraline treatments for 24 hours at various doses (0.5, 1, 2.5, 5 and 10 μM). For one hour, indatraline was administered to the indatraline + H2O2 group at various concentrations (0.5, 1, 2.5, 5 and 10 μM) before the group was subjected to 0.5 mM H2O2 for 24 hours. Following the occurrence of oxidative damage, total antioxidant status (TAS) and total oxidant status (TOS) levels were determined. Cell viability was also evaluated using the XTT assay. As a result, after hydrogen peroxide-induced oxidative damage, indatraline at doses of 10, 5, and 2.5 μM showed a protective effect by significantly enhanced cell survival in C6 cells(p < 0.001). Additionally, indatraline boosted the lowered TAS level while decreasing the elevated TOS levels following hydrogen peroxide-induced oxidative damage (p<0.001).","PeriodicalId":10906,"journal":{"name":"Cumhuriyet Science Journal","volume":"20 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139148560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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