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Rapid quantitative authentication and analysis of camellia oil adulterated with edible oils by electronic nose and FTIR spectroscopy 利用电子鼻和傅立叶变换红外光谱对掺入食用油的山茶油进行快速定量鉴定和分析
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100732
Xiaoran Wang , Yu Gu , Weiqi Lin , Qian Zhang

Camellia oil, recognized as a high-quality edible oil endorsed by the Food and Agriculture Organization, is confronted with authenticity issues arising from fraudulent adulteration practices. These practices not only pose health risks but also lead to economic losses. This study proposes a novel machine learning framework, referred to as a transformer encoder backbone with a support vector machine regressor (TES), coupled with an electronic nose (E-nose), for detecting varying adulteration levels in camellia oil. Experimental results indicate that the proposed TES model exhibits the best performance in identifying the adulterated concentration of camellia oi, compared with five other machine learning models (the support vector machine, random forest, XGBoost, K-nearest neighbors, and backpropagation neural network). The results obtained by E-nose detection are verified by complementary Fourier transform infrared (FTIR) spectroscopy analysis for identifying functional groups, ensuring accuracy and providing a comprehensive assessment of the types of adulterants. The proposed TES model combined with E-nose offers a rapid, effective, and practical tool for detecting camellia oil adulteration. This technique not only safeguards consumer health and economic interests but also promotes the application of E-nose in market supervision.

山茶油是联合国粮食及农业组织认可的优质食用油,但却面临着掺假欺诈行为带来的真实性问题。这些行为不仅会带来健康风险,还会造成经济损失。本研究提出了一种新颖的机器学习框架,即带有支持向量机回归器(TES)的变压器编码器主干,该框架与电子鼻(E-nose)相结合,用于检测山茶油中不同的掺假水平。实验结果表明,与其他五种机器学习模型(支持向量机、随机森林、XGBoost、K-近邻和反向传播神经网络)相比,所提出的 TES 模型在识别山茶油掺假浓度方面表现最佳。通过傅立叶变换红外光谱(FTIR)辅助分析来识别官能团,从而验证了电子鼻检测的结果,确保了准确性,并对掺假物的类型进行了全面评估。建议的 TES 模型与电子鼻相结合,为检测山茶油掺假提供了一种快速、有效和实用的工具。这项技术不仅能保障消费者的健康和经济利益,还能促进电子鼻在市场监管中的应用。
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引用次数: 0
Evaluation of the probiotic potential of yeast isolated from kombucha in New Zealand 评估从新西兰昆布茶中分离的酵母的益生菌潜力
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100711
Boying Wang , Kay Rutherfurd-Markwick , Ninghui Liu , Xue-Xian Zhang , Anthony N. Mutukumira

The current study investigated the in vitro probiotic potential of yeast isolated from kombucha, a tea beverage fermented with a symbiotic culture of acetic acid bacteria and yeast. A total of 62 yeast strains were previously isolated from four different commercial kombucha samples sold in New Zealand. Fifteen representative isolates belonging to eight different species were evaluated for their growth under different conditions (temperature, low pH, concentrations of bile salts, and NaCl). Cell surface characteristics, functional and enzymatic activities of the selected strains were also studied in triplicate experiments. Results showed that six strains (Dekkera bruxellensis LBY1, Sachizosaccharomyces pombe LBY5, Hanseniaspora valbyensis DOY1, Brettanomyces anomalus DOY8, Pichia kudraivzevii GBY1, and Saccharomyces cerevisiae GBY2) were able to grow under low-acid conditions (at pH 2 and pH 3) and in the presence of bile salts. This suggests their potential to survive passage through the human gut. All 15 strains exhibited negative enzymatic activity reactions (haemolytic, gelatinase, phospholipase, and protease activities), and thus, they can be considered safe to consume. Notably, two of the fifteen strains (Pichia kudraivzevii GBY1 and Saccharomyces cerevisiae GBY2) exhibited desirable cell surface hydrophobicity (64.60–83.87%), auto-aggregation (>98%), co-aggregation, resistance to eight tested antibiotics (ampicillin, chloramphenicol, colistin sulphate, kanamycin, nalidixic acid, nitrofurantoin, streptomycin, and tetracycline), and high levels of antioxidant activities (>90%). Together, our data reveal the probiotic activities of two yeast strains GBY1 and GBY2 and their potential application in functional food production.

康普茶是一种由醋酸菌和酵母菌共生培养发酵而成的茶饮料,本研究调查了从康普茶中分离出来的酵母菌的体外益生潜力。之前从新西兰销售的四种不同的商业昆布茶样品中分离出了共 62 株酵母菌。我们对属于 8 个不同物种的 15 个代表性分离菌株在不同条件(温度、低 pH 值、胆汁盐和氯化钠浓度)下的生长情况进行了评估。此外,还在一式三份的实验中研究了所选菌株的细胞表面特征、功能和酶活性。结果显示,六株菌株(Dekkera bruxellensis LBY1、Sachizosaccharomyces pombe LBY5、Hanseniaspora valbyensis DOY1、Brettanomyces anomalus DOY8、Pichia kudraivzevii GBY1 和 Saccharomyces cerevisiae GBY2)能够在低酸条件(pH 值为 2 和 3)和胆汁盐存在下生长。这表明它们有可能在人体肠道中存活下来。所有 15 株菌株都表现出负酶活性反应(溶血、明胶酶、磷脂酶和蛋白酶活性),因此可以放心食用。值得注意的是,15 株菌株中有两株(Pichia kudraivzevii GBY1 和 Saccharomyces cerevisiae GBY2)表现出理想的细胞表面疏水性(64.60-83.87%)、自动聚集(>98%)、共聚集、对八种测试抗生素(氨苄西林、氯霉素、硫酸秋水仙碱、卡那霉素、萘啶酸、硝基呋喃妥因、链霉素和四环素)的抗性以及高水平的抗氧化活性(>90%)。我们的数据揭示了 GBY1 和 GBY2 这两种酵母菌株的益生菌活性及其在功能食品生产中的潜在应用。
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引用次数: 0
Lipid incorporated biopolymer based edible films and coatings in food packaging: A review 食品包装中以脂质结合生物聚合物为基础的可食用薄膜和涂层:综述
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100720
L. Susmita Devi , Amit K. Jaiswal , Swarna Jaiswal

In the evolving landscape of food packaging, lipid-based edible films and coatings are emerging as a sustainable and effective solution for enhancing food quality and prolonging shelf life. This critical review aims to offer a comprehensive overview of the functional properties, roles, and fabrication techniques associated with lipid-based materials in food packaging. It explores the unique advantages of lipids, including waxes, resins, and fatty acids, in providing effective water vapor, gas, and microbial barriers. When integrated with other biopolymers, such as proteins and polysaccharides, lipid-based composite films demonstrate superior thermal, mechanical, and barrier properties. The review also covers the application of these innovative coatings in preserving a wide range of fruits and vegetables, highlighting their role in reducing moisture loss, controlling respiration rates, and maintaining firmness. Furthermore, the safety aspects of lipid-based coatings are discussed to address consumer and regulatory concerns.

在不断发展的食品包装领域,基于脂质的可食用薄膜和涂层正在成为提高食品质量和延长保质期的一种可持续的有效解决方案。本评论旨在全面概述食品包装中脂基材料的功能特性、作用和制造技术。它探讨了脂质(包括蜡、树脂和脂肪酸)在提供有效的水蒸气、气体和微生物屏障方面的独特优势。当与其他生物聚合物(如蛋白质和多糖)结合使用时,脂基复合薄膜可表现出卓越的热性能、机械性能和阻隔性能。本综述还介绍了这些创新涂层在保存各种水果和蔬菜方面的应用,强调了它们在减少水分流失、控制呼吸速率和保持坚硬度方面的作用。此外,还讨论了脂基涂层的安全问题,以解决消费者和监管机构的关切。
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引用次数: 0
Effect of cooking conditions on iron release from pots and development of kinetic models for iron supplementation in NIPs 烹饪条件对铁从锅中释放的影响以及开发用于在 NIP 中补充铁的动力学模型
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100830
Cuizhu Shi , Guangrong Zhe , Xiang Ding , Qian Meng , Jingpeng Li , Li Deng

The World Health Organization (WHO) has identified the use of iron cookware as a potential strategy for alleviating iron deficiency anaemia (IDA) and emphasises the need for action-oriented research in this area. In response to this need, our study systematically investigated the patterns of iron release from various types of cookware under different cooking conditions. Among these, nitrided iron pots (NIPs), the most widely used cookware, were selected for the development of kinetic models to predict iron release efficiently across a range of cooking temperatures and pH levels in food materials. Our results demonstrated that iron release from the pots was significantly influenced by cooking conditions such as the type of cookware, cooking temperatures, cooking times, types of acidic substances, and the pH of the cooking environment. Specifically, higher temperatures, longer cooking times, lower pH levels, and the presence of acetic acid were found to maximise iron release into food. We developed a series of kinetic models—Iron Release-Temperature Models (I, II, and III) and Iron Release-pH Models (IV, V, and VI)—to predict iron release from NIPs. The temperature models are applicable for cooking food with a pH of 5.00–6.00 within a temperature range of 50–100 °C, while the pH models are designed for food with a pH of 3.00–6.00 at boiling temperatures. Validation experiments confirmed the relative accuracy of these models. Additionally, when comparing the predicted iron release with the Recommended Nutrient Intake (RNI) guidelines, the findings support the efficacy of iron pots as a viable method for iron supplementation.

世界卫生组织(WHO)已将使用铁质炊具作为缓解缺铁性贫血(IDA)的潜在策略,并强调需要在这一领域开展以行动为导向的研究。针对这一需求,我们的研究系统地调查了不同烹饪条件下各类炊具的铁释放模式。在这些炊具中,我们选择了使用最广泛的氮化铁锅(NIPs),用于开发动力学模型,以有效预测食物材料在不同烹饪温度和 pH 值条件下的铁释放情况。我们的研究结果表明,锅具中铁的释放量受烹饪条件的显著影响,如锅具类型、烹饪温度、烹饪时间、酸性物质类型以及烹饪环境的 pH 值。具体来说,较高的温度、较长的烹饪时间、较低的 pH 值和醋酸的存在可最大限度地将铁释放到食物中。我们开发了一系列动力学模型--铁释放-温度模型(I、II、III)和铁释放-pH 模型(IV、V、VI)--来预测 NIPs 中铁的释放。温度模型适用于在 50-100 °C 温度范围内烹饪 pH 值为 5.00-6.00 的食物,而 pH 值模型则适用于在沸腾温度下烹饪 pH 值为 3.00-6.00 的食物。验证实验证实了这些模型的相对准确性。此外,在将预测的铁释放量与推荐营养摄入量(RNI)指南进行比较时,研究结果支持铁锅作为一种可行的补铁方法的有效性。
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引用次数: 0
Novel formulations for developing fresh hybrid cheese analogues utilizing fungal-fermented brewery side-stream flours 利用真菌发酵的酿酒厂副流粉开发新鲜混合奶酪类似物的新型配方
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100829
Anastasia Palatzidi , Olga Nikoloudaki , Maria Garcia Torreiro , Carolina Matteucci , Giovanna Ferrentino , Matteo Mario Scampicchio , Raffaella Di Cagno , Marco Gobbetti

This study investigated the development of hybrid cheese analogues (HCA) made with fermented brewery side-stream ingredients (spent yeast and malt rootlets) and dairy milk. Different percentages of side-stream flours (3.5%, 5%, and 7.5%) were mixed with pasteurized milk, and the developed HCA were evaluated for their biochemical and textural properties. The addition of a fermentation step improved nutrient availability and led to pH (range 4.79–5.60) and moisture content (range 45.86%–61.29%) similar to traditional animal-based fresh cheeses (control). The inclusion of side-stream flours led to coagulation, even without rennet addition. The higher the concentration of the flour used, the faster the coagulation time, suggesting synergistic effect between the enzymes of the rennet and the enzymes present in the fermented side-stream flours. Nevertheless, textural properties were inferior compared to the control. Selected HCA formulations with added 3.5% flour exhibited increased counts of enterococci and enterobacteria cell densities, ranging from 7.28 ± 0.03 to 7.72 ± 0.09 log CFU/g and 4.90 ± 0.16 to 5.41 ± 0.01 log CFU/g, respectively. Compared to the control sample, HCA formulations exhibited higher concentrations of organic acids, peptides, and free amino acids (FAAs). Lactic acid reached up to 23.78 ± 0.94 g/kg of dry matter (DM), while the peptide area reached up to 22918.50 ± 2370.93 mL⋅AU. Additionally, the total concentration of individual FAAs reached up to 2809.74 ± 104.85 mg/kg of DM, contrasted with the control, which resulted in lower concentrations (847.65 ± 0.02 mg/kg of DM). The overall findings suggested that despite challenges in microbiological quality and textural properties, HCA produced with the inclusion of up to 3.5% brewery side-stream flours could be a sustainable solution to produce nutritious dairy alternatives.

本研究调查了用发酵啤酒副产品配料(废酵母和麦芽根块)和牛奶制成的混合奶酪类似物(HCA)的开发情况。将不同比例的副产品面粉(3.5%、5% 和 7.5%)与巴氏杀菌奶混合,并对所开发的 HCA 的生化和质构特性进行了评估。添加发酵步骤提高了营养成分的可用性,pH 值(范围 4.79-5.60)和水分含量(范围 45.86%-61.29%)与传统的动物性新鲜奶酪(对照组)相似。即使不添加凝乳酶,加入侧流面粉也会导致凝结。使用的面粉浓度越高,凝固时间越快,这表明凝乳酶的酶和发酵侧流面粉中的酶之间存在协同效应。然而,与对照组相比,质地特性较差。添加了 3.5%面粉的 HCA 配方显示肠球菌和肠杆菌细胞密度增加,分别为 7.28 ± 0.03 至 7.72 ± 0.09 log CFU/g 和 4.90 ± 0.16 至 5.41 ± 0.01 log CFU/g。与对照样品相比,HCA 配方中有机酸、肽和游离氨基酸的浓度更高。乳酸达到 23.78 ± 0.94 克/千克干物质(DM),而肽的含量达到 22918.50 ± 2370.93 毫升-AU。此外,单个 FAAs 的总浓度高达 2809.74 ± 104.85 毫克/千克 DM,而对照组的浓度较低(847.65 ± 0.02 毫克/千克 DM)。总体研究结果表明,尽管在微生物质量和质地特性方面存在挑战,但在生产 HCA 时加入高达 3.5% 的酿酒厂副流粉,不失为一种生产营养乳品替代品的可持续解决方案。
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引用次数: 0
High throughput parameter estimation and uncertainty analysis applied to the production of mycoprotein from synthetic lignocellulosic hydrolysates 应用于利用合成木质纤维素水解物生产菌体蛋白的高通量参数估计和不确定性分析
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100908
Mason Banks , Mark Taylor , Miao Guo
The current global food system produces substantial waste and carbon emissions while exacerbating the effects of global hunger and protein deficiency. This study aims to address these challenges by exploring the use of lignocellulosic agricultural residues as feedstocks for microbial protein fermentation, focusing on Fusarium venenatum A3/5, a mycelial strain known for its high protein yield and nutritional quality. We propose a high throughput microlitre batch fermentation system paired with analytical chemistry to generate time series data of microbial growth and substrate utilisation. An unstructured biokinetic model was developed using a bootstrap sampling approach to quantify uncertainty in the parameter estimates. The model was validated against an independent data set of a different glucose-xylose composition to assess the predictive performance. Our results indicate a robust model fit with high coefficients of determination and low root mean squared errors for biomass, glucose, and xylose concentrations. Estimated parameter values provided insights into the resource utilisation strategies of Fusarium venenatum A3/5 in mixed substrate cultures, aligning well with previous research findings. Significant correlations between estimated parameters were observed, highlighting challenges in parameter identifiability. The high throughput workflow presents a novel, rapid methodology for biokinetic model development, enabling efficient exploration of microbial growth dynamics and substrate utilisation. This innovative method directly supports the development of a foundational model for optimising microbial protein production from lignocellulosic hydrolysates, contributing to a more sustainable global food system.
当前的全球粮食系统产生了大量废物和碳排放,同时加剧了全球饥饿和蛋白质缺乏的影响。本研究旨在通过探索使用木质纤维素农作物残留物作为微生物蛋白质发酵的原料来应对这些挑战,重点是以高蛋白产量和营养质量著称的菌丝菌株 Fusarium venenatum A3/5。我们提出了一种高通量微升批量发酵系统,并将其与分析化学相结合,以生成微生物生长和底物利用的时间序列数据。我们利用引导取样法开发了一个非结构化生物动力学模型,以量化参数估计的不确定性。该模型通过不同葡萄糖-木糖组成的独立数据集进行了验证,以评估其预测性能。结果表明,模型拟合稳健,生物量、葡萄糖和木糖浓度的决定系数高,均方根误差小。估计的参数值有助于深入了解文氏镰刀菌 A3/5 在混合基质培养中的资源利用策略,这与之前的研究结果非常吻合。估计参数之间存在显著的相关性,凸显了参数可识别性方面的挑战。高通量工作流程为生物动力学模型的开发提供了一种新颖、快速的方法,可有效探索微生物的生长动力学和基质利用。这种创新方法直接支持了从木质纤维素水解物中优化微生物蛋白质生产的基础模型的开发,有助于建立更可持续的全球食品系统。
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引用次数: 0
Acetate cellulose fibrous scaffold is suitable for cultivated fat production 醋酸纤维素纤维支架适用于培养脂肪的生产
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100903
Amanda Maria Siqueira Moreira , Júlia Meireles Nogueira , Jade Carceroni , Jorge Luís Guadalupe , Ana Elisa Antunes dos Santos , Ana Maria Alvarenga Fagundes , Aline Gonçalves Lio Copola , Gerluza Aparecida Borges Silva , Aline Bruna da Silva , João Paulo Ferreira Santos , Juliano Douglas Silva Albergaria , Luciana de Oliveira Andrade , Erika Cristina Jorge
Fat is an essential component of meat which contributes to its sensory characteristics. Therefore, producing cultivated fat is essential to replicate the texture, flavor, and juiciness of conventional meat. One of the challenges in obtaining cultivated fat is that once adipocytes reach differentiation in culture, they tend to float. In this study, we tested whether immortalized pre-adipocytes could be viable, grow, and differentiate when cultivated onto a fibrous scaffold produced by the electrospun of cellulose acetate. Our results demonstrated that the cells attach, proliferate, colonize, and differentiate into mature adipocytes in the three-dimensional fibrous structure during the culture period. Moreover, when layers of the scaffold containing differentiated cells were stacked, it acquired a characteristic similar to conventional animal fat. Therefore, this research suggests that fibrous scaffolds produced using cellulose acetate are a promising substrate for producing cultivated fat.
脂肪是肉类的重要组成部分,它有助于肉类的感官特征。因此,生产培养脂肪对于复制传统肉类的质地、风味和多汁性至关重要。获得培养脂肪的挑战之一是脂肪细胞一旦在培养过程中达到分化,就容易漂浮起来。在这项研究中,我们测试了永生化前脂肪细胞在醋酸纤维素电纺丝制成的纤维支架上培养时能否存活、生长和分化。我们的结果表明,在培养期间,细胞在三维纤维结构中附着、增殖、定殖并分化为成熟的脂肪细胞。此外,当含有分化细胞的支架层层叠加时,它获得了与传统动物脂肪相似的特征。因此,这项研究表明,使用醋酸纤维素制作的纤维支架是一种很有前景的生产培养脂肪的基质。
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引用次数: 0
Understanding the role of CaCl2 in salt substitute for low-salt and high-quality surimi products 了解 CaCl2 在低盐优质鱼糜产品代盐中的作用
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100877
Xinyan Tong , Yijin Liu , Ganping Wei , Nasra Seif Juma , Fang Tian , Dieynabou Diao , Meiling Chen , Bin Zheng , Yadong Zhao
Salt substitute has been widely used to prepare low-salt foods due to potential health benefits, though the role of CaCl2 in salt substitute and its unique impacts on food quality have been rarely investigated. In this study, comprehensive research has been conducted to elucidate the effects of replacing NaCl with varying concentrations of CaCl2 on the surimi gel characteristics. The introduction of CaCl2 interacted with surimi proteins differently from NaCl, thus leading to difference in protein aggregation behaviors and surimi gel properties. It has been found that a proper proportion of CaCl2 for NaCl substitution could create salt bridges between surimi proteins more effectively, resulting in an ordered, smooth and dense gel network with an increased water holding capacity (WHC) and improved gel strength. Furthermore, TGase activated by Ca2+ boosted the formation of ε-(γ-glutamyl) lysine bonds, which cross-linked surimi proteins to form a firm gel with a better three-dimensional structure. However, replacing NaCl with excessive amount of CaCl2 as divalent salts induced more serious protein aggregation, leading to water loss and gel properties deterioration. More specially, replacing NaCl with CaCl2 at 50% showed the best performance, as evidenced by the most abundant disulfide bonds and hydrophobic interactions, highest hardness and chewiness, and greatest storage modulus. This study provided new insights on developing high-quality surimi gels with significantly reduced salt concentration and improved gel characteristics.
由于潜在的健康益处,代盐已被广泛用于制备低盐食品,但人们很少研究 CaCl2 在代盐中的作用及其对食品质量的独特影响。本研究进行了全面研究,以阐明用不同浓度的 CaCl2 替代 NaCl 对鱼糜凝胶特性的影响。引入的 CaCl2 与鱼糜蛋白质的相互作用与 NaCl 不同,从而导致蛋白质聚集行为和鱼糜凝胶特性的差异。研究发现,用适当比例的 CaCl2 替代 NaCl 能更有效地在鱼糜蛋白质之间建立盐桥,从而形成有序、光滑和致密的凝胶网络,并提高持水量(WHC)和凝胶强度。此外,Ca2+激活的TG酶还能促进ε-(γ-谷氨酰)赖氨酸键的形成,从而交联鱼糜蛋白,形成具有更好三维结构的坚固凝胶。然而,用过多的二价盐 CaCl2 取代 NaCl 会诱发更严重的蛋白质聚集,导致水分流失和凝胶性能下降。更特别的是,用 50%的 CaCl2 取代 NaCl 的性能最佳,表现在二硫键和疏水作用最丰富、硬度和咀嚼性最高、储存模量最大。这项研究为开发盐浓度显著降低、凝胶特性得到改善的优质鱼糜凝胶提供了新的见解。
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引用次数: 0
Sini San ameliorates lipid metabolism in hyperprolactinemia rat with liver-depression 四逆散可改善高泌乳素血症大鼠肝郁的脂质代谢
IF 6.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100853
Weidong Xu , Shasha Tian , Guanqun Mao , Yu Li , Hua Qian , Wenhua Tao

Sini San (SNS) is used to treat liver depression and is applied in both food and herbal medicine. Hyperprolactinemia (HPRL) is a common endocrine disorder, and patients with HPRL are usually associated with depressive symptoms. However, whether SNS is effective in treating HPRL combined with liver depression and its underlying mechanisms are unknown. We applied network pharmacology and molecular docking to predict the mechanism of SNS for the treatment of liver-depressed HPRL. Therapeutic effects were validated in animal models and cells. Metabolomics was also used to evaluate the effect of SNS on liver-depressed HPRL. Network pharmacology and molecular docking analysis showed that AKT1, TNF and IL6 were the key targets, and SNS improved depressive behaviors, regulated sex hormone levels, and improved ovarian morphology. Combined network pharmacology and metabolomics analyses showed that SNS could act by regulating lipid metabolism. In addition, SNS significantly reduced the release of prolactin (PRL) in rat pituitary tumor MMQ cells. Overall, SNS can significantly treat HPRL liver depression at both animal and cellular levels, and effectively alleviate the related symptoms by regulating lipid metabolism. AKT1, TNF and IL6 may be key targets.

四逆散(SNS)用于治疗肝郁,在食品和中药中都有应用。高泌乳素血症(HPRL)是一种常见的内分泌疾病,HPRL 患者通常伴有抑郁症状。然而,SNS 是否能有效治疗 HPRL 合并肝郁及其内在机制尚不清楚。我们应用网络药理学和分子对接技术预测了SNS治疗肝郁型HPRL的机制。治疗效果在动物模型和细胞中得到了验证。代谢组学也被用来评估SNS对肝衰竭HPRL的影响。网络药理学和分子对接分析表明,AKT1、TNF和IL6是关键靶点,SNS能改善抑郁行为、调节性激素水平并改善卵巢形态。结合网络药理学和代谢组学分析表明,SNS 可通过调节脂质代谢发挥作用。此外,SNS还能明显减少大鼠垂体瘤MMQ细胞中催乳素(PRL)的释放。总之,SNS能在动物和细胞水平上显著治疗HPRL肝郁,并通过调节脂质代谢有效缓解相关症状。AKT1、TNF和IL6可能是关键靶点。
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引用次数: 0
Composite alginate-based hydrogel delivery of antioxidant pumpkin protein hydrolysate in simulated gastrointestinal condition 在模拟胃肠道条件下用海藻酸盐复合水凝胶输送抗氧化剂南瓜蛋白水解物
IF 6.3 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-01-01 DOI: 10.1016/j.crfs.2024.100739
Zeinab Nooshi Manjili , Alireza Sadeghi Mahoonak , Mohammad Ghorbani , Hoda Shahiri Tabarestani , Vahid Erfani Moghadam

Pumpkin seeds are rich in protein (24–36.5%). Some of them are consumed as nuts, while others are regarded as waste and used for feeding animals. Protein hydrolysates from pumpkin seeds possess some bioactive properties, such as anti-oxidant activity. In this work, various composite alginate hydrogels contain Aloe vera, CMC, and tragacanth have been employed to protect PSPH against degradation in simulated gastrointestinal digestion (SGI) and regulate its release rate. The encapsulation efficiency of PSPH in plain alginate and beads with Aloe vera, CMC, and tragacanth combinations was 71.63, 75.63, 85.07, and 80.4%, respectively. The release rate of the plain alginate beads was %30.23 in the SGF and %52.26 in the SIF, and decreased in the composite-based beads. The highest decreasing rate in the antioxidant activity during SGI was observed in free PSPH, and the decreasing rate slowed down in the alginate-based composites. The swelling rate in plain alginate was %-23.43 and %25.43 in the SGF and SIF, respectively, and increased in the composite-based beads. The FTIR spectra of hydrogels before and after loading with PSPH showed identical absorption patterns and were similar to each other. Based on the data for SEM, it was revealed that substituting other polymers in polymer combinations with alginates resulted in a porosity reduction of the beads and smoother and more uniform surfaces. Based on the results, the combination of polysacchared with alginate could protect and increase the applicability of PSPH as a functional component in the food industry.

南瓜籽富含蛋白质(24-36.5%)。其中一些作为坚果食用,而另一些则被视为废物,用于喂养动物。南瓜籽的蛋白质水解物具有一些生物活性特性,如抗氧化活性。在这项工作中,采用了含有芦荟、CMC 和黄蓍的各种复合海藻酸水凝胶来保护南瓜籽蛋白水解物,防止其在模拟胃肠道消化(SGI)过程中降解,并调节其释放速度。PSPH在普通海藻酸盐和含有芦荟、CMC和黄曲霉的珠子中的包封效率分别为71.63%、75.63%、85.07%和80.4%。在 SGF 和 SIF 中,普通海藻酸盐珠的释放率分别为 30.23%和 52.26%,而在基于复合材料的珠子中,释放率则有所下降。在 SGI 期间,游离 PSPH 的抗氧化活性下降率最高,而海藻酸盐基复合材料的下降率则有所减缓。在 SGF 和 SIF 中,普通海藻酸盐的溶胀率分别为 %-23.43 和 %25.43,而在基于复合材料的珠子中,溶胀率有所上升。添加 PSPH 前后水凝胶的傅立叶变换红外光谱显示出相同的吸收模式,且彼此相似。根据扫描电子显微镜的数据显示,用藻酸盐替代聚合物组合中的其他聚合物会使珠子的孔隙率降低,表面更加光滑和均匀。根据研究结果,聚苯乙烯与海藻酸盐的组合可保护并提高聚苯乙烯-苯乙烯作为功能性成分在食品工业中的适用性。
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Current Research in Food Science
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