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Surgical Advancements, Immunotherapy, Targeted and Conventional Therapies, Biopsy, Colposcopy, and Pap Smear Integration in the Management of Cervical Cancer.
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-22 DOI: 10.2174/0109298673337745241123054840
Siddhi Wargantiwar, Sankha Bhattacharya, Abhishek Kanugo

Cervical cancer remains a significant global health concern, making it essential to investigate new treatment options continuously. This page provides an overview of the latest advancements and best practices in detection and intervention, including Pap smears, colposcopy, biopsy, immunotherapy, targeted therapies, chemotherapy, radiation therapy, and surgery. Surgical techniques such as radical hysterectomy and minimally invasive procedures have advanced to enhance patient outcomes and quality of life. Simultaneously, radiation therapy methods have been refined to maximize tumour control while reducing adverse effects. Chemotherapy remains vital, with new drugs and combination regimens demonstrating improved tolerance and efficacy. Immunotherapy, notably immune checkpoint inhibitors, has shown promise in advanced stages of cervical cancer. Additionally, targeted therapies that focus on specific biochemical pathways offer the potential for personalized treatment approaches. This review critically assesses ongoing research, evaluates existing data, and emphasizes the opportunities and challenges of each therapeutic approach. Ultimately, integrating these diverse treatment strategies is the key to enhancing patient outcomes.

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引用次数: 0
Research Prospects for Necroptosis in Inflammatory Diseases: A Bibliometric Analysis.
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-22 DOI: 10.2174/0109298673336964250103060730
Ya Ting Tan, Mei Juan Wang, Shu Chao Wang

Background: Necroptosis is a modifiable form of cell death mainly dependent on RIPK3 and MLKL. The association between necroptosis and inflammation has been a key focus of research. An increasing number of studies have shown that necroptosis plays an important role in inflammatory diseases, such as inflammatory bowel disease.

Methods: Articles published up to 2023 were searched on the Web of Science. VOSviewer, CiteSpace, Gephi, and Microsoft Office Excel were used for bibliometric analysis and visualisation. In addition, journal impact factors and journal partitions were obtained through the Web of Science.

Results: A total of 3011 articles were included in this study. The number of publications and citations in the field increased year by year. China had the highest number of publications. Cell Death & Disease published the most papers in the field. P. Vandenabeele is one of the most important scholars in this field. The most cited reference was "Molecular Mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death".We found substantial evidence that acute kidney injury, sepsis, cancer, and other diseases are closely related to necroptosis. In addition, we found that inhibitors of necroptosis have great potential in the treatment of inflammatory diseases.

Conclusion: This is the first bibliometric analysis of studies related to necroptosis in inflammatory diseases. Our results provide an overview of basic and influential research, providing a basis for the identification of valuable research directions. Furthermore, this work offers general insight into the role of necroptosis in inflammatory human diseases.

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引用次数: 0
SQSTM1/P62 Mediates the Effects of CPNE3 on the Epithelialmesenchymal Transition and Migratory Inhibition of Lung Adenocarcinoma Cells.
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-21 DOI: 10.2174/0109298673340242250102104725
Yanping Li, You Li, Liming Xu, Guangming Yang, Huansi Zhou, Mingjing Jin, Kai Yu, Chunhua Lu

Introduction: Copine-3 (CPNE3) is a conservative calcium-dependent phospholipid-binding protein belonging to the copines protein family. CPNE3 has been implicated in the development and progression of several diseases, including cancer.

Method: Herein, we investigated the molecular mechanisms through which CPNE3 regulates the migration of lung adenocarcinoma (LUAD) cells in vitro. Western blotting and immunohistochemical assays showed that CPNE3 is widely distributed in LUAD tissues and cell lines and that CPNE3 downregulation promotes the migration of human LUAD A549 cells.

Results: Stable isotope labelling with amino acids in cell culture, which is a quantitative proteomics approach coupled with bioinformatic analyses, revealed that CPNE3 regulates SQSTM1/p62 and vimentin expression, indicating that CPNE3 may mediate epithelial-mesenchymal transition (EMT). CPNE3 silencing by siRNA upregulated vimentin levels but downregulated E-cadherin levels in the A549 cells.

Conclusion: Furthermore, SQSTM1/p62 knockdown enhanced migratory ability and EMT progression in CPNE3-silenced A549 cells. Overall, CPNE3 knockdown was found to promote EMT by inhibiting SQSTM1/p62 signalling and facilitating cell migration. Our findings highlight the role of CPNE3 as a tumour suppressor, providing deeper insights into its carcinogenic roles in LUAD.

{"title":"SQSTM1/P62 Mediates the Effects of CPNE3 on the Epithelialmesenchymal Transition and Migratory Inhibition of Lung Adenocarcinoma Cells.","authors":"Yanping Li, You Li, Liming Xu, Guangming Yang, Huansi Zhou, Mingjing Jin, Kai Yu, Chunhua Lu","doi":"10.2174/0109298673340242250102104725","DOIUrl":"https://doi.org/10.2174/0109298673340242250102104725","url":null,"abstract":"<p><strong>Introduction: </strong>Copine-3 (CPNE3) is a conservative calcium-dependent phospholipid-binding protein belonging to the copines protein family. CPNE3 has been implicated in the development and progression of several diseases, including cancer.</p><p><strong>Method: </strong>Herein, we investigated the molecular mechanisms through which CPNE3 regulates the migration of lung adenocarcinoma (LUAD) cells in vitro. Western blotting and immunohistochemical assays showed that CPNE3 is widely distributed in LUAD tissues and cell lines and that CPNE3 downregulation promotes the migration of human LUAD A549 cells.</p><p><strong>Results: </strong>Stable isotope labelling with amino acids in cell culture, which is a quantitative proteomics approach coupled with bioinformatic analyses, revealed that CPNE3 regulates SQSTM1/p62 and vimentin expression, indicating that CPNE3 may mediate epithelial-mesenchymal transition (EMT). CPNE3 silencing by siRNA upregulated vimentin levels but downregulated E-cadherin levels in the A549 cells.</p><p><strong>Conclusion: </strong>Furthermore, SQSTM1/p62 knockdown enhanced migratory ability and EMT progression in CPNE3-silenced A549 cells. Overall, CPNE3 knockdown was found to promote EMT by inhibiting SQSTM1/p62 signalling and facilitating cell migration. Our findings highlight the role of CPNE3 as a tumour suppressor, providing deeper insights into its carcinogenic roles in LUAD.</p>","PeriodicalId":10984,"journal":{"name":"Current medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrated Transcriptome and Proteome Analyses Reveal Differentially Expressed Genes and Proteins in Granulosa Cells from Female Patients with Metabolic Syndrome-associated Infertility. 综合转录组和蛋白质组分析揭示了女性代谢综合征相关不孕症患者颗粒细胞中差异表达的基因和蛋白质。
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-20 DOI: 10.2174/0109298673357582241223070335
Fangli Dong, Wanjun Zhang, Bo Sun, Wenbin Niu, Jun Zhai, Yihong Guo, Fang Wang

Background: Metabolic Syndrome (MS) is a cluster of conditions that significantly increase the risk of infertility in women. Granulosa cells are crucial for ovarian folliculogenesis and fertility. Understanding molecular alterations in these cells can provide insights into MS-associated infertility.

Objective: This study aimed to investigate Differentially Expressed Genes (DEGs) and Proteins (DEPs) in granulosa cells from female patients with MS-associated infertility.

Method: Transcriptome and proteome analyses were integrated to compare granulosa cells from three MS patients with infertility to three control subjects. RNA sequencing and quantitative proteomics analyses were conducted, followed by differential expression analysis, Gene Set Enrichment Analysis (GSEA), and Protein-protein Interaction (PPI) network construction. Functional enrichment of overlapping DEGs and DEPs and potential drug-protein interactions were also explored. Hub genes identified by PPI were validated via quantitative Polymerase Chain Reaction (qPCR) and western blot assays.

Results: Principal Component Analysis (PCA) demonstrated a distinct separation between MS and control groups, indicating significant differences in gene and protein expression. A total of 1,046 upregulated and 23 downregulated DEGs, along with 222 upregulated and 412 downregulated DEPs, were identified in the MS group. GSEA highlighted enrichment in processes, like the cell cycle and immune response. Venn diagram revealed 71 overlapping DEGs and DEPs, mainly related to immune regulation. Key hub proteins and potential therapeutic candidates were identified, with hub genes upregulated at the mRNA level, but downregulated at the protein level in granulosa cells of MS patients.

Conclusion: The integrative analyses revealed significant molecular alterations in granulosa cells from MS patients with infertility. Identified DEGs, DEPs, and hub proteins suggested potential therapeutic targets and pathways for addressing MS-associated infertility.

背景:代谢综合征(MS)是一组显著增加女性不孕风险的疾病。颗粒细胞对卵巢卵泡发生和生育至关重要。了解这些细胞的分子变化可以为ms相关的不孕症提供见解。目的:研究女性ms相关性不孕症患者颗粒细胞中的差异表达基因(DEGs)和差异表达蛋白(DEPs)。方法:采用转录组学和蛋白质组学相结合的方法,比较3例多发性硬化症不孕患者与3例对照组的颗粒细胞。进行RNA测序和定量蛋白质组学分析,然后进行差异表达分析、基因集富集分析(GSEA)和蛋白-蛋白相互作用(PPI)网络构建。我们还探讨了重叠DEGs和DEPs的功能富集以及潜在的药物-蛋白相互作用。通过定量聚合酶链反应(quantitative Polymerase Chain Reaction, qPCR)和免疫印迹(western blot)对PPI鉴定的枢纽基因进行验证。结果:主成分分析(PCA)显示MS组与对照组之间存在明显的分离,表明基因和蛋白表达存在显著差异。MS组共鉴定出1046个deg上调和23个下调,以及222个dep上调和412个dep下调。GSEA强调在细胞周期和免疫应答等过程中富集。Venn图显示71个deg和dep重叠,主要与免疫调节有关。关键枢纽蛋白和潜在的治疗候选者被确定,枢纽基因在mRNA水平上调,但在MS患者颗粒细胞的蛋白水平下调。结论:综合分析显示,多发性硬化症患者的颗粒细胞发生了显著的分子改变。已鉴定的DEGs、DEPs和hub蛋白提示了治疗ms相关性不孕症的潜在治疗靶点和途径。
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引用次数: 0
Identification of Key Genes and Pathways in Lenvatinib-resistant Hepatocellular Carcinoma using Bioinformatic Analysis and Experimental Validation. 利用生物信息学分析和实验验证鉴定lenvatinib耐药肝细胞癌的关键基因和通路。
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-20 DOI: 10.2174/0109298673329991241111111941
Ming Yang, Zhaoyue Wang, Riga Su, Dongbing Li, Jun Zhou

Background: Resistance to lenvatinib poses a serious threat to the therapy of patients with Hepatocellular Carcinoma (HCC). The mechanism by which HCC develops resistance to lenvatinib is currently unknown.

Objective: The aim of this study was to identify key genes and pathways involved in lenvatinib resistance in HCC using bioinformatic analysis and experimental validation.

Methods: Differentially expressed genes (DEGs) were identified from the GSE186191 gene expression profile, comparing HCC cell lines with lenvatinib-resistant HCC cell lines. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were then carried out using DAVID. A protein-protein interaction network was constructed to visualize DEGs and identify hub genes. The expression and prognostic significance of these hub genes were further examined. Additionally, genomic enrichment analysis (GSEA) was utilized to investigate the potential functions of key genes. Following this, the presence of AHSG was validated in both the original Huh7 cells and the lenvatinib-resistant Huh7 (Huh7LR) cells resistant to lenvatinib through the utilization of quantitative real-time PCR (qRT-PCR).

Results: A total of 232 DEGs were identified between HCC cell lines and those that are resistant to lenvatinib. These DEGs were significantly associated with arrhythmogenic right ventricular cardiomyopathy, hypertrophic cardiomyopathy, dilated cardiomyopathy, and mucin-type O-glycan biosynthesis. Three hub genes, including AHSG, C6, and ORM1, were identified. The low expression of AHSG showed a poorer prognosis in HCC. GSEA demonstrated a significant correlation between low AHSG expression and pathways involving fatty acid metabolism, ribosome function, glycine, serine, and threonine metabolism, peroxisome activity, and bile acid biosynthesis. The expression of AHSG was notably reduced in Huh7LR cells (p = 0.006) compared to Huh7 cells.

Conclusion: Diminished AHSG expression is strongly associated with lenvatinib resistance in HCC, suggesting that it may have implications for developing effective strategies to overcome this resistance.

背景:lenvatinib耐药严重威胁肝细胞癌(HCC)患者的治疗。HCC对lenvatinib产生耐药性的机制目前尚不清楚。目的:本研究的目的是通过生物信息学分析和实验验证,确定HCC中lenvatinib耐药的关键基因和途径。方法:从GSE186191基因表达谱中鉴定差异表达基因(DEGs),并将HCC细胞系与lenvatinib耐药HCC细胞系进行比较。然后使用DAVID对基因本体和京都基因与基因组百科全书进行分析。构建蛋白相互作用网络,实现DEGs可视化和枢纽基因识别。进一步研究这些中心基因的表达及其预后意义。此外,利用基因组富集分析(GSEA)来研究关键基因的潜在功能。随后,利用实时荧光定量PCR (qRT-PCR)技术,在原始Huh7细胞和对lenvatinib耐药的Huh7 (Huh7LR)细胞中验证了AHSG的存在。结果:在HCC细胞系和lenvatinib耐药细胞系之间共鉴定出232个deg。这些deg与致心律失常性右室心肌病、肥厚性心肌病、扩张性心肌病和粘蛋白型o -聚糖生物合成显著相关。鉴定出三个枢纽基因,包括AHSG、C6和ORM1。AHSG低表达在HCC中预后较差。GSEA表明,低AHSG表达与脂肪酸代谢、核糖体功能、甘氨酸、丝氨酸和苏氨酸代谢、过氧化物酶体活性和胆汁酸生物合成等途径之间存在显著相关性。AHSG在Huh7LR细胞中的表达明显低于Huh7细胞(p = 0.006)。结论:AHSG表达减少与HCC中lenvatinib耐药密切相关,提示这可能对开发克服这种耐药的有效策略具有重要意义。
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引用次数: 0
The Effect of Gallic Acid on the Alleviation of the Chemotherapy-Induced Myelosuppression. 没食子酸在缓解化疗诱导的骨髓抑制中的作用。
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-20 DOI: 10.2174/0109298673354122241220051407
Junyi Luo, Zhaoxia Zhang, Liming Jin, Zhaoying Wang, Qiuyue Sun, Dawei He

Objective: This study aims to investigate the effect of Gallic Acid (GA) on the alleviation of chemotherapy-induced bone marrow suppression, with a comparison to Diyu sheng bai tablets (DYSB) and RhG-CSF.

Methods: A mouse model of bone marrow suppression was established in BALB/c mice using intraperitoneal injections of cyclophosphamide (CTX). All procedures were performed after obtaining ethical clearance from the institutional animal ethics committee. Mice were treated with low (100 mg/kg/d), medium (200 mg/kg/d), and high (400 mg/kg/d) doses of Gallic Acid (GA) to mitigate CTX-induced bone marrow suppression. In parallel, mice in the positive control group were also treated with DYSB and RhG-CSF at their respective standard doses (DYSB: 100 mg/kg/day, RhG-CSF: 125 mg/kg/day). The efficacy of GA in alleviating chemotherapy-induced bone marrow suppression was evaluated through blood cell counts, immune organ (thymus and spleen) indices, bone marrow nucleated cell (BMNC) counts, cell cycle analysis, apoptosis, histopathology of bone marrow and spleen, and analysis of splenic hematopoietic factors.

Results: CTX induced a decrease in peripheral blood cells and BMNC counts, reduced spleen and thymus indices, and diminished abnormal pathology of bone marrow and spleen, as well as decreasing disturbances in hematopoietic factors. GA was able to alleviate these abnormalities in the bone marrow. It modulated cell proliferation and apoptosis, adjusted the proportion of cells in the G0/G1 phase, and reduced apoptosis in femoral bone marrow.

Conclusion: Gallic Acid (GA) alleviates chemotherapy-induced bone marrow suppression by improving immune organ function, promoting bone marrow cell recovery, and inhibiting apoptosis. These findings support GA as a potential adjunct therapy for chemotherapy, with promising clinical applications.

目的:探讨没食子酸(GA)对化疗诱导的骨髓抑制的缓解作用,并与降郁生白片(DYSB)和RhG-CSF进行比较。方法:采用腹腔注射环磷酰胺(CTX)建立BALB/c小鼠骨髓抑制模型。所有程序均在获得机构动物伦理委员会的伦理许可后进行。小鼠分别接受低剂量(100 mg/kg/d)、中剂量(200 mg/kg/d)和高剂量(400 mg/kg/d)没食子酸(GA)治疗,以减轻ctx诱导的骨髓抑制。同时,阳性对照组小鼠也分别给予DYSB和RhG-CSF标准剂量(DYSB: 100 mg/kg/day, RhG-CSF: 125 mg/kg/day)。通过血细胞计数、免疫器官(胸腺和脾脏)指数、骨髓有核细胞(BMNC)计数、细胞周期分析、细胞凋亡、骨髓和脾脏组织病理学、脾造血因子分析来评价GA缓解化疗诱导的骨髓抑制的疗效。结果:CTX诱导外周血细胞和BMNC计数减少,脾脏和胸腺指数降低,骨髓和脾脏异常病理减轻,造血因子干扰减少。GA能够减轻骨髓中的这些异常。调节细胞增殖和凋亡,调节G0/G1期细胞比例,减少股骨髓细胞凋亡。结论:没食子酸(GA)通过改善免疫器官功能、促进骨髓细胞恢复、抑制细胞凋亡等途径缓解化疗诱导的骨髓抑制。这些发现支持GA作为化疗的潜在辅助疗法,具有良好的临床应用前景。
{"title":"The Effect of Gallic Acid on the Alleviation of the Chemotherapy-Induced Myelosuppression.","authors":"Junyi Luo, Zhaoxia Zhang, Liming Jin, Zhaoying Wang, Qiuyue Sun, Dawei He","doi":"10.2174/0109298673354122241220051407","DOIUrl":"https://doi.org/10.2174/0109298673354122241220051407","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the effect of Gallic Acid (GA) on the alleviation of chemotherapy-induced bone marrow suppression, with a comparison to Diyu sheng bai tablets (DYSB) and RhG-CSF.</p><p><strong>Methods: </strong>A mouse model of bone marrow suppression was established in BALB/c mice using intraperitoneal injections of cyclophosphamide (CTX). All procedures were performed after obtaining ethical clearance from the institutional animal ethics committee. Mice were treated with low (100 mg/kg/d), medium (200 mg/kg/d), and high (400 mg/kg/d) doses of Gallic Acid (GA) to mitigate CTX-induced bone marrow suppression. In parallel, mice in the positive control group were also treated with DYSB and RhG-CSF at their respective standard doses (DYSB: 100 mg/kg/day, RhG-CSF: 125 mg/kg/day). The efficacy of GA in alleviating chemotherapy-induced bone marrow suppression was evaluated through blood cell counts, immune organ (thymus and spleen) indices, bone marrow nucleated cell (BMNC) counts, cell cycle analysis, apoptosis, histopathology of bone marrow and spleen, and analysis of splenic hematopoietic factors.</p><p><strong>Results: </strong>CTX induced a decrease in peripheral blood cells and BMNC counts, reduced spleen and thymus indices, and diminished abnormal pathology of bone marrow and spleen, as well as decreasing disturbances in hematopoietic factors. GA was able to alleviate these abnormalities in the bone marrow. It modulated cell proliferation and apoptosis, adjusted the proportion of cells in the G0/G1 phase, and reduced apoptosis in femoral bone marrow.</p><p><strong>Conclusion: </strong>Gallic Acid (GA) alleviates chemotherapy-induced bone marrow suppression by improving immune organ function, promoting bone marrow cell recovery, and inhibiting apoptosis. These findings support GA as a potential adjunct therapy for chemotherapy, with promising clinical applications.</p>","PeriodicalId":10984,"journal":{"name":"Current medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elemene Injection Suppresses Pancreatic Cancer Progress through Regulating Cell Adhesion: A Research Based upon Network Pharmacology and Verification Test. 榄香烯注射液通过调节细胞粘附抑制胰腺癌进展:基于网络药理学和验证试验的研究。
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-20 DOI: 10.2174/0109298673351591241114101143
Jiangang Zhao, Fenglin Zhang, Ping Li

Background: This study investigates the potential effects of elemene injection on pancreatic cancer using network pharmacology and experimental validation.

Methods: GEO database were used to acquire genes which are differentially expressed between pancreatic cancer tissue and normal tissue. The vigorous energetic ingredients were identified in research and the object genes were obtained from BATMAN-TCM. The key targets and signaling pathways of elemene injection were identified using compound- target network analysis, protein-protein interaction network analysis, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. in vitro experiments were carried out to confirm the accuracy of the network pharmacology predictions.

Results: Two hundred and eleven target genes that may be involved in Elemene's impact on pancreatic cancer were identified. Bioinformatics analysis was conducted to determine the two active mixtures and one key target. GO and KEGG enrichment analyses indicated that elemene injection exerts therapeutic effects on pancreatic cancer, regulating the cell adhesion by ECM-receptor interaction pathway. The experiments verified that elemene injection suppressed the growth and movement of pancreatic cancer cell lines Panc02 and MiaPaca-2 and the mechanism is related to regulating ECM-receptor interaction pathway-related genes. FN1 was identified as core targets by bioinformatics analysis. The FN1 was downregulated by elemene injection and was validated by QPCR and Western Blot.

Conclusion: The findings of the current study emphasized that elemene injection might control cell attachment, decrease metastasis, and suppresses pancreatic cancer progress. FN1 might be a therapeutic target for pancreatic cancer.

背景:本研究采用网络药理学和实验验证的方法探讨榄香烯注射液对胰腺癌的潜在作用。方法:利用GEO数据库获取胰腺癌组织与正常组织的差异表达基因。在研究中鉴定了具有旺盛活性的中药成分,并获得了目标基因。采用复合靶点网络分析、蛋白-蛋白相互作用网络分析、基因本体和京都基因与基因组百科全书途径富集分析等方法,对榄香烯注射液的关键靶点和信号通路进行了鉴定。体外实验证实了网络药理学预测的准确性。结果:鉴定出111个可能参与榄香烯对胰腺癌影响的靶基因。生物信息学分析确定了两种活性混合物和一个关键靶点。GO和KEGG富集分析表明榄香烯注射液对胰腺癌具有治疗作用,通过ecm受体相互作用途径调节细胞粘附。实验证实榄香烯注射抑制胰腺癌细胞株Panc02和MiaPaca-2的生长和运动,其机制与调控ecm受体相互作用通路相关基因有关。通过生物信息学分析确定FN1为核心靶点。榄香烯注射液下调FN1表达,并通过QPCR和Western Blot验证。结论:本研究结果强调榄香烯注射可能控制细胞附着,减少转移,抑制胰腺癌进展。FN1可能是胰腺癌的治疗靶点。
{"title":"Elemene Injection Suppresses Pancreatic Cancer Progress through Regulating Cell Adhesion: A Research Based upon Network Pharmacology and Verification Test.","authors":"Jiangang Zhao, Fenglin Zhang, Ping Li","doi":"10.2174/0109298673351591241114101143","DOIUrl":"https://doi.org/10.2174/0109298673351591241114101143","url":null,"abstract":"<p><strong>Background: </strong>This study investigates the potential effects of elemene injection on pancreatic cancer using network pharmacology and experimental validation.</p><p><strong>Methods: </strong>GEO database were used to acquire genes which are differentially expressed between pancreatic cancer tissue and normal tissue. The vigorous energetic ingredients were identified in research and the object genes were obtained from BATMAN-TCM. The key targets and signaling pathways of elemene injection were identified using compound- target network analysis, protein-protein interaction network analysis, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. in vitro experiments were carried out to confirm the accuracy of the network pharmacology predictions.</p><p><strong>Results: </strong>Two hundred and eleven target genes that may be involved in Elemene's impact on pancreatic cancer were identified. Bioinformatics analysis was conducted to determine the two active mixtures and one key target. GO and KEGG enrichment analyses indicated that elemene injection exerts therapeutic effects on pancreatic cancer, regulating the cell adhesion by ECM-receptor interaction pathway. The experiments verified that elemene injection suppressed the growth and movement of pancreatic cancer cell lines Panc02 and MiaPaca-2 and the mechanism is related to regulating ECM-receptor interaction pathway-related genes. FN1 was identified as core targets by bioinformatics analysis. The FN1 was downregulated by elemene injection and was validated by QPCR and Western Blot.</p><p><strong>Conclusion: </strong>The findings of the current study emphasized that elemene injection might control cell attachment, decrease metastasis, and suppresses pancreatic cancer progress. FN1 might be a therapeutic target for pancreatic cancer.</p>","PeriodicalId":10984,"journal":{"name":"Current medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Expression of the LDLR, LDLRAP1, and PCSK9 Genes has Prognostic Significance in Triple-negative Breast Cancer. LDLR、LDLRAP1和PCSK9基因表达在三阴性乳腺癌中的预后意义
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-20 DOI: 10.2174/0109298673291217241219055416
Ivan Denisovich Antipenko, Darya Mikhailovna Olkhovik, Olga Nikolaevna Solopova, Gulfia Amirovna Khayretdinova, Olga Sergeevna Kalacheva, Julia Alekseevna Makarova, Maxim Yurievich Shkurnikov

Aims: The purpose of this study was to investigate the prognostic significance of cholesterol uptake genes in predicting the survival of breast cancer patients.

Background: Cholesterol plays a crucial role in the homeostasis of tumor cells. It is known that cholesterol levels can influence important parameters of the disease, such as sensitivity to therapy, progression, and metastasis of cancer. Previous studies suggest that breast cancer subtypes exhibit differences in metabolism.

Objective: The objectives of this study were to determine whether cholesterol uptake genes have prognostic significance for overall survival in breast cancer patients, evaluate if this prognostic significance varies between breast cancer subtypes, and identify differences in the expression of cholesterol uptake genes among these subtypes.

Methods: Data from mRNA sequencing of tumors from the Cancer Genome Atlas (TCGA) portal were analyzed. Tumors were classified into molecular subtypes, and the prognostic significance of cholesterol uptake gene expression levels was evaluated for each subtype. DESeq2 and Fisher's test were used to assess differences in gene expression.

Results: High expression levels of genes involved in de novo cholesterol synthesis were associated with poor prognosis for the Basal-like and Luminal A breast cancer subtypes. The prognostic significance of low-density lipoprotein receptor (LDLR), LDLR adapter protein 1 (LDLRAP1), and proprotein convertase subtilisin/kexin type 9 (PCSK9), which are responsible for exogenous cholesterol uptake, varied across subtypes. Specifically, low expression of LDLR was associated with a favorable prognosis for the luminal A (OR = 2.17; FDR = 0.0048) and luminal B (OR = 2.21; FDR = 0.015) subtypes but indicated poor prognosis in the basal-like subtype (OR = 0.48; FDR = 0.05). No genes were significant for prognosis prediction in the HER2-positive subtype. The HER2+ subtype exhibited higher expression of cholesterol uptake genes compared to the basal-like subtype based on the analysis of tumor mRNA sequencing (OR = 6.45, p-value = 3.07E-05). This finding was also confirmed through the study of publicly available single-cell sequencing data (OR = 40.3, p-value = 2.19e-07), which may contribute to the differences in their prognostic significance.

Conclusion: The prognostic significance of cholesterol uptake gene expression varies among breast cancer subtypes. Precise fitting of biomarkers into breast cancer subtypes may aid in more accurate patient stratification and improve treatment approaches.

目的:本研究的目的是探讨胆固醇摄取基因在预测乳腺癌患者生存中的预后意义。背景:胆固醇在肿瘤细胞的稳态中起着至关重要的作用。众所周知,胆固醇水平可以影响疾病的重要参数,如对治疗的敏感性、癌症的进展和转移。先前的研究表明,乳腺癌亚型在代谢方面表现出差异。目的:本研究的目的是确定胆固醇摄取基因对乳腺癌患者总生存是否具有预后意义,评估这种预后意义在乳腺癌亚型之间是否存在差异,并确定这些亚型中胆固醇摄取基因的表达差异。方法:对来自癌症基因组图谱(TCGA)门户网站的肿瘤mRNA测序数据进行分析。将肿瘤分为分子亚型,并评估每种亚型中胆固醇摄取基因表达水平对预后的意义。采用DESeq2和Fisher检验法评估基因表达差异。结果:参与从头合成胆固醇的基因的高表达水平与基底样和腔A乳腺癌亚型的不良预后相关。负责外源性胆固醇摄取的低密度脂蛋白受体(LDLR)、LDLR适配蛋白1 (LDLRAP1)和蛋白转化酶枯草素/酮素9型(PCSK9)的预后意义在不同亚型之间存在差异。具体来说,LDLR的低表达与腔内a的良好预后相关(OR = 2.17;FDR = 0.0048)和luminal B (OR = 2.21;FDR = 0.015)亚型,但基底样亚型预后较差(OR = 0.48;FDR = 0.05)。没有基因对her2阳性亚型的预后预测有显著意义。基于肿瘤mRNA测序分析,HER2+亚型比基底样亚型表现出更高的胆固醇摄取基因表达(OR = 6.45, p值= 3.07E-05)。这一发现也通过公开的单细胞测序数据得到了证实(OR = 40.3, p值= 2.19e-07),这可能导致了它们在预后意义上的差异。结论:胆固醇摄取基因表达在乳腺癌亚型中的预后意义不同。精确拟合生物标志物到乳腺癌亚型可能有助于更准确的患者分层和改进治疗方法。
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引用次数: 0
Exploring PANoptosis Related Novel Diagnostic Biomarkers and Potential Drugs for Sarcopenia based on Machine Learning and Experimental Validation. 基于机器学习和实验验证的PANoptosis相关新诊断生物标志物和潜在药物的探索。
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-20 DOI: 10.2174/0109298673341863241210112605
Zhibo Deng, Chao Song, Rongsheng Zhang, Yu Xiu, Linhai Yang, Hanhao Dai, Jun Luo, Jie Xu

Background: Sarcopenia, an aseptic chronic inflammatory disease, is a complex and debilitating disease characterized by the progressive degeneration of skeletal muscle. PANoptosis, a novel proinflammatory programmed cell death pathway, has been linked to various diseases. However, the precise role of PANoptosis-related features in sarcopenia remains uncertain.

Methods: According to the intersection of differentially expressed genes (DEGs) in the sarcopenia dataset GSE167186 and the PANoptosis gene set, we classified patients into PANoptosis-related subtypes (PANRS) using consensus clustering. The DEGs of PANRS were intersected with weighted gene co-expression network analysis (WGCNA). Proteinprotein interaction network and cytoHubba algorithms were employed to further identify potential genes related to PANoptosis. The most characteristic genes were selected using LASSO regression and validated by ROC curve analysis, followed by relevant immune infiltration analysis. Additionally, small-molecule drug screening was performed using Cmap. The relative expression levels of hub genes in sarcopenia were confirmed by PCR. Finally, single-cell analysis and GSEA were used to examine the distribution and function of hub genes.

Results: Thirty-five candidate genes were identified through WGCNA and PANRS. Machine learning and ROC curve analysis revealed three core genes: LTBP2, ETS2, and H3.3B, all of which were up-regulated in patients with sarcopenia (p<0.01). Immune infiltration analysis indicated that these three diagnostic genes were linked to the activation of NK cells and macrophages. Single-cell analysis demonstrated that LTBP2 was mainly localized in fibroblasts, while ETS2 and H3.3B exhibited a uniform distribution. Enrichment analysis indicated that the three hub genes were predominantly associated with the inhibition of energy metabolism.

Conclusion: In this study, the hub genes LTBP2, ETS2, and H3.3B associated with PANoptosis in sarcopenia were successfully identified through a combination of bioinformatics and experimental verification methods. This establishes a foundation for new candidate diagnostic and therapeutic targets for sarcopenia.

背景:骨骼肌减少症是一种无菌性慢性炎症性疾病,是一种以骨骼肌进行性变性为特征的复杂和衰弱性疾病。PANoptosis是一种新的促炎程序性细胞死亡途径,与多种疾病有关。然而,panoposis相关特征在肌肉减少症中的确切作用仍不确定。方法:根据肌少症数据集GSE167186中差异表达基因(DEGs)与PANoptosis基因集的交叉,采用共识聚类方法将患者分为PANoptosis相关亚型(PANRS)。用加权基因共表达网络分析(WGCNA)对PANRS的deg进行交叉分析。利用蛋白相互作用网络和cytoHubba算法进一步鉴定与PANoptosis相关的潜在基因。采用LASSO回归筛选最具特征的基因,进行ROC曲线分析验证,并进行相关免疫浸润分析。此外,使用Cmap进行小分子药物筛选。聚合酶链反应证实hub基因在肌少症中的相对表达量。最后,利用单细胞分析和GSEA对枢纽基因的分布和功能进行了研究。结果:通过WGCNA和PANRS鉴定出35个候选基因。机器学习和ROC曲线分析揭示了三个核心基因LTBP2、ETS2和H3.3B在肌少症患者中均上调(p结论:本研究通过生物信息学和实验验证相结合的方法,成功鉴定了肌少症PANoptosis相关的枢纽基因LTBP2、ETS2和H3.3B。这为肌少症新的候选诊断和治疗靶点奠定了基础。
{"title":"Exploring PANoptosis Related Novel Diagnostic Biomarkers and Potential Drugs for Sarcopenia based on Machine Learning and Experimental Validation.","authors":"Zhibo Deng, Chao Song, Rongsheng Zhang, Yu Xiu, Linhai Yang, Hanhao Dai, Jun Luo, Jie Xu","doi":"10.2174/0109298673341863241210112605","DOIUrl":"https://doi.org/10.2174/0109298673341863241210112605","url":null,"abstract":"<p><strong>Background: </strong>Sarcopenia, an aseptic chronic inflammatory disease, is a complex and debilitating disease characterized by the progressive degeneration of skeletal muscle. PANoptosis, a novel proinflammatory programmed cell death pathway, has been linked to various diseases. However, the precise role of PANoptosis-related features in sarcopenia remains uncertain.</p><p><strong>Methods: </strong>According to the intersection of differentially expressed genes (DEGs) in the sarcopenia dataset GSE167186 and the PANoptosis gene set, we classified patients into PANoptosis-related subtypes (PANRS) using consensus clustering. The DEGs of PANRS were intersected with weighted gene co-expression network analysis (WGCNA). Proteinprotein interaction network and cytoHubba algorithms were employed to further identify potential genes related to PANoptosis. The most characteristic genes were selected using LASSO regression and validated by ROC curve analysis, followed by relevant immune infiltration analysis. Additionally, small-molecule drug screening was performed using Cmap. The relative expression levels of hub genes in sarcopenia were confirmed by PCR. Finally, single-cell analysis and GSEA were used to examine the distribution and function of hub genes.</p><p><strong>Results: </strong>Thirty-five candidate genes were identified through WGCNA and PANRS. Machine learning and ROC curve analysis revealed three core genes: LTBP2, ETS2, and H3.3B, all of which were up-regulated in patients with sarcopenia (p<0.01). Immune infiltration analysis indicated that these three diagnostic genes were linked to the activation of NK cells and macrophages. Single-cell analysis demonstrated that LTBP2 was mainly localized in fibroblasts, while ETS2 and H3.3B exhibited a uniform distribution. Enrichment analysis indicated that the three hub genes were predominantly associated with the inhibition of energy metabolism.</p><p><strong>Conclusion: </strong>In this study, the hub genes LTBP2, ETS2, and H3.3B associated with PANoptosis in sarcopenia were successfully identified through a combination of bioinformatics and experimental verification methods. This establishes a foundation for new candidate diagnostic and therapeutic targets for sarcopenia.</p>","PeriodicalId":10984,"journal":{"name":"Current medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LGALS3BP: A Potential Prognostic Biomarker Influencing Antitumor Immunity in Triple-negative Breast Cancer. LGALS3BP:影响三阴性乳腺癌抗肿瘤免疫的潜在预后生物标志物
IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-20 DOI: 10.2174/0109298673367980250101053748
Anqi Hu, Shuaikang Pan, Yuan He, XueRu Wang, Dong Qian, Xiaoyang Li

Objective: LGALS3BP exhibits differential expression in various types of tumors. This study aimed to analyze its potential diagnostic and prognostic value in Triple- negative Breast Cancer (TNBC).

Methods: We conducted a comprehensive analysis of LGALS3BP's differential expression and its association with patient survival outcomes using data from public databases. To further validate these findings, Immunohistochemistry (IHC) experiments were performed to confirm the differential expression of LGALS3BP protein in TNBC. Additionally, we also investigated the relationship among LGALS3BP, tumor immune infiltration, and drug sensitivity.

Results: Results indicated LGALS3BP to be significantly upregulated in TNBC, with its high expression correlating with improved survival outcomes. Furthermore, LGALS3BP expression correlated with immune cell infiltration. Notably, high LGALS3BP expression may confer a greater likelihood of benefiting from immunotherapy.

Conclusion: LGALS3BP may serve as a diagnostic and prognostic biomarker for TNBC.

目的:LGALS3BP在不同类型肿瘤中的表达存在差异。本研究旨在分析其在三阴性乳腺癌(TNBC)中的潜在诊断和预后价值。方法:利用公共数据库的数据,我们对LGALS3BP的差异表达及其与患者生存结果的关系进行了全面分析。为了进一步验证这些发现,我们进行了免疫组织化学(IHC)实验来证实LGALS3BP蛋白在TNBC中的差异表达。此外,我们还研究了LGALS3BP与肿瘤免疫浸润和药物敏感性的关系。结果:结果表明,LGALS3BP在TNBC中显著上调,其高表达与生存结果的改善相关。此外,LGALS3BP表达与免疫细胞浸润相关。值得注意的是,高LGALS3BP表达可能更有可能从免疫治疗中获益。结论:LGALS3BP可作为TNBC的诊断和预后生物标志物。
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Current medicinal chemistry
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