Developmental biology最新文献

Heterozygous R391 TUBB4B pathogenic variations are responsible for an association of hearing loss and retinal dystrophy in human. With the goal of understanding the functions of TuBB4b and the pathogenic role of R391 variations, we characterized tubB4B in zebrafish and identified the gene regulatory elements necessary and sufficient for expression of TubB4b as in endogenous tissues. Using knock-out and transgenic approaches, we determined that R391 mutations impair neither localization of TubB4B within sensory hair cells (SHC) nor their structure, but induced to a small decrease in SHC number from anterior crista. Expression of R391 mutations in sensory hair cells has no effect on zebrafish audition, suggesting a different equilibrium between various tubulin isotypes in zebrafish possibly due to compensatory mechanisms. The careful expression analysis and transgenic tools generated in this study could help understand how recently described pathogenic variants lead to more severe clinical forms of TUBB4B-related diseases.

The surface of epithelial tissues is covered by an apical extracellular matrix (aECM). The aECMs of different tissues have distinct compositions to serve distinct functions, yet how a particular cell type assembles the proper aECM is not well understood. We used the cell type-specific matrix of the C. elegans vulva to investigate the connection between cell identity and matrix assembly. The vulva is an epithelial tube composed of seven cell types descending from EGFR/Ras-dependent (1°) and Notch-dependent (2°) lineages. Vulva aECM contains multiple Zona Pellucida domain (ZP) proteins, which are a common component of aECMs across life. ZP proteins LET-653 and CUTL-18 assemble on 1° cell surfaces, while NOAH-1 assembles on a subset of 2° surfaces. All three ZP genes are broadly transcribed, indicating that cell type-specific ZP assembly must be determined by features of the destination cell surface. The paired box (Pax) transcription factor EGL-38 promotes assembly of 1° matrix and prevents inappropriate assembly of 2° matrix, suggesting that EGL-38 promotes expression of one or more ZP matrix organizers. Our results connect the known signaling pathways and various downstream effectors to EGL-38/Pax expression and the ZP matrix component of vulva cell fate execution. We propose that dedicated transcriptional networks may contribute to cell-appropriate assembly of aECM in many epithelial organs.

The trophectoderm (TE) epithelium forms the outer layer of the mammalian blastocyst and generates the blastocoel through vectorial transport. Its differentiation during cleavage, studied mainly in mouse, is integrated with blastocyst morphogenesis with key roles for cell polarisation, asymmetric cell divisions, cell signalling, regulatory transcription factors and cellular inheritance. The TE provides a physical and cellular protection to the emerging lineages of the embryo essential for the integrity of blastocyst development. Here, two examples of TE differentiation are considered in some detail where this immediate protective function for embryo survival is assessed: (i) cellular processes from TE at the polar-mural junctional zone in the early blastocyst that later form filopodia traversing the blastocoel, and (ii) the endocytic system which matures and polarises during differentiation. Understanding the broad role for TE in regulating early morphogenesis and environmental protection of the embryo, including these two examples, have clinical as well as biological relevance.

The sense of taste is mediated primarily by taste buds on the tongue. These multicellular sensory organs are induced, patterned and become innervated during embryogenesis such that a functional taste system is present at birth when animals begin to feed. While taste buds have been considered ectodermal appendages, this is only partly accurate as only fungiform taste buds in the anterior tongue arise from the ectoderm. Taste buds found in the posterior tongue actually derive from endoderm. Nonetheless, both anterior and posterior buds are functionally similar, despite their disparate embryonic origins. In this review, I compare the development of ectodermal vs endodermal taste buds, highlighting the many differences in the cellular and molecular genetic mechanisms governing their formation.

Developmental Biology is intricately connected to current issues of societal and political importance. Bringing these connections into the classroom makes the biology topics relevant to our students and, when students find classroom topics relevant, they are more likely to think critically about the material. In addition, this type of engagement encourages students to remain in science majors and pursue careers in biological and biomedical fields. The use of guided inquiry methodology, scaffolded discussions, and a variety of source material on sex and gender, has been successful in engaging students in an undergraduate Developmental Biology course.