Pub Date : 2020-07-09DOI: 10.29235/1561-8323-2020-64-3-317-324
Н. Г. Аверина, А. В. Емельянова, Т. Г. Каляга, С. М. Савина
The effect of exogenous 5-aminolevulinic acid (ALA) on the activity of dihydroflavonol-4-reductase (DFR), the expression of the dfr gene and the hy5 gene of the transcription factor Hy5 and the light effect of different intensities in combination with the ALA action on the accumulation of anthocyanins in cotyledonous leaves of winter rape (Brassica napus L.) were studied. It was shown that the stimulation of the accumulation of anthocyanins under the exogenous ALA action at the molecular level was provided by increasing the expression level of the dfr and hy5 genes and the activity of the DFR enzyme. Increasing the light intensity from 40.5 to 66.2 μmol photons/m 2 ·s enhanced the ability of plants to accumulate anthocyanins on average by 35 %. The ALA action at concentrations of 50, 100, 150 and 200 mg/L led to an additional increase in the accumulation of anthocyanins at the two used levels of illumination, and in a dose-dependent manner. The stimulating effect of ALA under high light intensity was much higher than in the case of lower illumination. Thus, the stimulation of the anthocyanin accumulation under illumination of 40.5 μmol photons/m 2 ·s was 106 % when using 50 mg/L ALA, 165 % – when using 100 mg/L ALA, 222 % – in the case of 150 mg/L ALA and 350 % – under the action of 200 mg/L ALA compared with light control without of ALA treatment. At an illumination of 66.2 μmol photons/m 2 ·s, these indicators were 164, 262, 359 and 583 % respectively. Thus, it was demonstrated that the stimulation of the accumulation of anthocyanins under the action of ALA in winter rape plants was due to its positive effect on the transcription of the dfr and hy5 genes at the molecular level.
{"title":"Молекулярно-генетические механизмы регуляции дигидрофлавонол редуктазы и транскрипционного фактора Hy5 экзогенной 5-аминолевулиновой кислотой в проростках озимого рапса","authors":"Н. Г. Аверина, А. В. Емельянова, Т. Г. Каляга, С. М. Савина","doi":"10.29235/1561-8323-2020-64-3-317-324","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-3-317-324","url":null,"abstract":"The effect of exogenous 5-aminolevulinic acid (ALA) on the activity of dihydroflavonol-4-reductase (DFR), the expression of the dfr gene and the hy5 gene of the transcription factor Hy5 and the light effect of different intensities in combination with the ALA action on the accumulation of anthocyanins in cotyledonous leaves of winter rape (Brassica napus L.) were studied. It was shown that the stimulation of the accumulation of anthocyanins under the exogenous ALA action at the molecular level was provided by increasing the expression level of the dfr and hy5 genes and the activity of the DFR enzyme. Increasing the light intensity from 40.5 to 66.2 μmol photons/m 2 ·s enhanced the ability of plants to accumulate anthocyanins on average by 35 %. The ALA action at concentrations of 50, 100, 150 and 200 mg/L led to an additional increase in the accumulation of anthocyanins at the two used levels of illumination, and in a dose-dependent manner. The stimulating effect of ALA under high light intensity was much higher than in the case of lower illumination. Thus, the stimulation of the anthocyanin accumulation under illumination of 40.5 μmol photons/m 2 ·s was 106 % when using 50 mg/L ALA, 165 % – when using 100 mg/L ALA, 222 % – in the case of 150 mg/L ALA and 350 % – under the action of 200 mg/L ALA compared with light control without of ALA treatment. At an illumination of 66.2 μmol photons/m 2 ·s, these indicators were 164, 262, 359 and 583 % respectively. Thus, it was demonstrated that the stimulation of the accumulation of anthocyanins under the action of ALA in winter rape plants was due to its positive effect on the transcription of the dfr and hy5 genes at the molecular level.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"24 1","pages":"317-324"},"PeriodicalIF":0.0,"publicationDate":"2020-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84841593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-08DOI: 10.29235/1561-8323-2020-64-3-273-281
М. А. Моховиков, О. В. Мильчанин, И. Н. Пархоменко, Фадей Фадеевич Комаров, Л. А. Власукова, Д. С. Королев, А. В. Мудрый, В. Д. Живулько, Арно Янсе ван Вуурен
The phase-structural composition of a silica film grown on Si substrate implanted with Zn ions at room temperature with different fluences has been studied using transmission electron microscopy and electron diffraction. The small clusters (1–2 nm) and the large clusters (5–7 nm) have been formed in as-implanted silica films with the Zn concentration of 6–7 at % and 16–18 at %, respectively. Furnace annealing at 750 °С for two hours results both in the formation of the orthorhombic Zn 2 SiO 4 phase (space group R-3) in the case of low fluence (5 · 10 16 cm –2 ) and in the formation of the cubic ZnO phase (space group F-43m) in the case of high fluence (1 · 10 17 cm –2 ). It has been shown that impurity loss during implantation and subsequent annealing increase with fluence of implanted ions. The fraction of Zn atoms in clusters has been estimated to be 15 % and 18 % for fluences (5 · 10 16 cm –2 ) and (1 · 10 17 cm –2 ), respectively. It has been shown that residual Zn impurities dissolved in silica matrix noticeably suppress the light-emitting properties of silica with embedded Zn 2 SiO 4 and ZnO nanocrystals.
用透射电镜和电子衍射研究了室温下注入不同影响的Zn离子在Si衬底上生长的硅膜的相结构组成。当锌浓度为6-7 at %和16-18 at %时，在注入二氧化硅薄膜中形成了小团簇(1 - 2nm)和大团簇(5 - 7nm)。在750°С下进行2小时的炉内退火，在低通量(5·10 16 cm -2)和高通量(1·10 17 cm -2)下形成正交Zn 2 sio4相(空间群R-3)和立方ZnO相(空间群F-43m)。结果表明，随着注入离子的影响，注入和退火过程中的杂质损失增加。据估计，在影响(5·10 16 cm -2)和(1·10 17 cm -2)下，簇中锌原子的比例分别为15%和18%。结果表明，溶解在二氧化硅基体中的残余Zn杂质明显抑制了嵌入zn2、sio4和ZnO纳米晶二氧化硅的发光性能。
{"title":"Структурные и оптические свойства оксида кремния, имплантированного ионами цинка: влияние степени пересыщения и термообработки","authors":"М. А. Моховиков, О. В. Мильчанин, И. Н. Пархоменко, Фадей Фадеевич Комаров, Л. А. Власукова, Д. С. Королев, А. В. Мудрый, В. Д. Живулько, Арно Янсе ван Вуурен","doi":"10.29235/1561-8323-2020-64-3-273-281","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-3-273-281","url":null,"abstract":"The phase-structural composition of a silica film grown on Si substrate implanted with Zn ions at room temperature with different fluences has been studied using transmission electron microscopy and electron diffraction. The small clusters (1–2 nm) and the large clusters (5–7 nm) have been formed in as-implanted silica films with the Zn concentration of 6–7 at % and 16–18 at %, respectively. Furnace annealing at 750 °С for two hours results both in the formation of the orthorhombic Zn 2 SiO 4 phase (space group R-3) in the case of low fluence (5 · 10 16 cm –2 ) and in the formation of the cubic ZnO phase (space group F-43m) in the case of high fluence (1 · 10 17 cm –2 ). It has been shown that impurity loss during implantation and subsequent annealing increase with fluence of implanted ions. The fraction of Zn atoms in clusters has been estimated to be 15 % and 18 % for fluences (5 · 10 16 cm –2 ) and (1 · 10 17 cm –2 ), respectively. It has been shown that residual Zn impurities dissolved in silica matrix noticeably suppress the light-emitting properties of silica with embedded Zn 2 SiO 4 and ZnO nanocrystals.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"517 1","pages":"273-281"},"PeriodicalIF":0.0,"publicationDate":"2020-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78149611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-17DOI: 10.29235/1561-8323-2020-64-2-135-143
П. П. Матус, С. В. Лемешевский
The stability with respect to coefficients of solution of a difference scheme approximating the initial boundary-value problem for the one-dimensional semi-linear hyperbolic equation is studied. The estimates of the solutions of both differential and difference problems are obtained. In the domain of existence of the solution, the estimates for perturbation of the solution of a difference scheme with respect to perturbation of the coefficients of the equation are obtained. These estimates are consistent with the estimates for the differential problem. In all cases, the method of energy inequalities, the Bihari inequality and its mesh analogue are used.
{"title":"Коэффициентная устойчивость решений разностных схем, аппроксимирующих смешанные задачи для полулинейных гиперболических уравнений","authors":"П. П. Матус, С. В. Лемешевский","doi":"10.29235/1561-8323-2020-64-2-135-143","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-2-135-143","url":null,"abstract":"The stability with respect to coefficients of solution of a difference scheme approximating the initial boundary-value problem for the one-dimensional semi-linear hyperbolic equation is studied. The estimates of the solutions of both differential and difference problems are obtained. In the domain of existence of the solution, the estimates for perturbation of the solution of a difference scheme with respect to perturbation of the coefficients of the equation are obtained. These estimates are consistent with the estimates for the differential problem. In all cases, the method of energy inequalities, the Bihari inequality and its mesh analogue are used.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"50 1","pages":"135-143"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74748858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-17DOI: 10.29235/1561-8323-2020-64-2-225-232
S. Lysenko, V. Loginov
The article analyzes the relationship between the forest cover and the amount of summer rainfalls in Belarus. We found that the spatial structure of the long-term precipitation field in Belarus is largely explained by the spatial features of its forest cover. In particular, the high forest cover in summer time provides 5–15 % more rain falls than that without forest. We also showed that the extremely dry period from 2014 to 2015 led to a significant transformation of the summer rainfall field. As a result, the field becomes almost the mirror opposite to the summer air temperature field. This indicates the important role of local evaporation in the formation of precipitation in the summer months. The important conclusion of the results is that additional forest stands are needed to prevent a further decrease in the level of surface and ground waters in Belarus. We also need to increase the use of agroforestry techniques in crop cultivation.
{"title":"Role of forests in maintaining a water balance in the territory of Belarus","authors":"S. Lysenko, V. Loginov","doi":"10.29235/1561-8323-2020-64-2-225-232","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-2-225-232","url":null,"abstract":"The article analyzes the relationship between the forest cover and the amount of summer rainfalls in Belarus. We found that the spatial structure of the long-term precipitation field in Belarus is largely explained by the spatial features of its forest cover. In particular, the high forest cover in summer time provides 5–15 % more rain falls than that without forest. We also showed that the extremely dry period from 2014 to 2015 led to a significant transformation of the summer rainfall field. As a result, the field becomes almost the mirror opposite to the summer air temperature field. This indicates the important role of local evaporation in the formation of precipitation in the summer months. The important conclusion of the results is that additional forest stands are needed to prevent a further decrease in the level of surface and ground waters in Belarus. We also need to increase the use of agroforestry techniques in crop cultivation.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"102 1","pages":"225-232"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78024180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-17DOI: 10.29235/1561-8323-2020-64-2-159-163
E. Kokoulina, M. Levchuk, M. Nevmerzhitsky, R. Shulyakovsky
In the framework of the diagrammatic approach, the total cross section of near-threshold π – photoproduction on the deuteron is calculated. Contributions of the diagrams corresponding to the plane wave impulse approximation as well as to NN - and π N -interactions in the final state have been taken into account. We have compared the theoretical predictions with the results of the recent measurements performed at the MAX IV laboratory of the Lund University. There is good agreement with the data at photon energies from 147 to 155 MeV. However, at 158 and 160 MeV, the theoretical predictions significantly overestimate the measured cross sections.
在图解法的框架下，计算了近阈值π -在氘核上产生的总横截面。图的贡献对应于平面波脉冲近似以及NN -和π N -相互作用在最终状态已被考虑。我们将理论预测与隆德大学MAX IV实验室最近进行的测量结果进行了比较。与光子能量147 ~ 155 MeV的数据吻合较好。然而，在158和160 MeV时，理论预测明显高估了测量截面。
{"title":"Total cross section of the reaction γd → π–pp in the threshold energy region","authors":"E. Kokoulina, M. Levchuk, M. Nevmerzhitsky, R. Shulyakovsky","doi":"10.29235/1561-8323-2020-64-2-159-163","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-2-159-163","url":null,"abstract":"In the framework of the diagrammatic approach, the total cross section of near-threshold π – photoproduction on the deuteron is calculated. Contributions of the diagrams corresponding to the plane wave impulse approximation as well as to NN - and π N -interactions in the final state have been taken into account. We have compared the theoretical predictions with the results of the recent measurements performed at the MAX IV laboratory of the Lund University. There is good agreement with the data at photon energies from 147 to 155 MeV. However, at 158 and 160 MeV, the theoretical predictions significantly overestimate the measured cross sections.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"273 1","pages":"159-163"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83040683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-17DOI: 10.29235/1561-8323-2020-64-2-164-172
A. Kraskouski, V. Kulikouskaya, O. Molchan, K. Hileuskaya, V. Yurin, V. Agabekov
Hydrogel negatively charged (–13.5 ± 5.0 mV) calcium pectinate nano- and submicroparticles (50–150 nm) were obtained. A technique for entrapment of a plant growth regulator (trans-cinnamic acid) in the particles up to 40 wt. % has been developed. It has been established that the complete release of trans-cinnamic acid in the Murashige–Skoog medium takes 2.5 hours. The obtained particles of calcium pectinate do not affect the growth processes of cells in suspension culture and can be used as neutral carriers for growth regulators.
{"title":"Fabrication and properties of calcium pectinate hydrogel nanoparticles with trans-cinnamic acid","authors":"A. Kraskouski, V. Kulikouskaya, O. Molchan, K. Hileuskaya, V. Yurin, V. Agabekov","doi":"10.29235/1561-8323-2020-64-2-164-172","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-2-164-172","url":null,"abstract":"Hydrogel negatively charged (–13.5 ± 5.0 mV) calcium pectinate nano- and submicroparticles (50–150 nm) were obtained. A technique for entrapment of a plant growth regulator (trans-cinnamic acid) in the particles up to 40 wt. % has been developed. It has been established that the complete release of trans-cinnamic acid in the Murashige–Skoog medium takes 2.5 hours. The obtained particles of calcium pectinate do not affect the growth processes of cells in suspension culture and can be used as neutral carriers for growth regulators.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"13 1","pages":"164-172"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75569504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-17DOI: 10.29235/1561-8323-2020-64-2-245-256
I. Sheyko, E. Yanovich, N. V. Pristupa, T. N. Timoshenko, R. Sheyko, I. V. Anihovskaya, K. A. Kаpshevich
The genealogical breed structure is represented by 5 main factory lines: Zubr, Zenit, Zaslon, Zont, Zefir. The genealogical structure of the Belarusian meat breed is formed using the generally accepted selection methods, including the assessment by pedigree, strict selection, targeted selection, the assessment of replacement young by productivity on elevers, the assessment of boars and queens by the offspring quality using the control feeding method. The targeted breeding work with herds of Belarusian meat breed pigs during a long period of time according to the long-term target program allows us to obtain large boars and queens that are characterized by the meat-type body. When assessing boar-producers of the Belarusian meat breed aged 36 months and older in all farms the values of the body live weight and length were on the average 305.3 kg and 179 cm respectively. As a result of studying the reproductive qualities of animals, it is established that, on the average, in the queens selected into the leading group in all farms, the indicators of prolificacy, the weight of one young pig at birth, the number of young pigs aged 21 days and milking ability were 11.74 animals and 1.11 kg, 10.06 animals and 54.34 respectively. The highest growth rate from birth to a live weight of 100 kg was seen in the Zefir line animals – 550 g. The indicator of the growth in reaching a live weight of 100 kg was 181 days. The superiority over the mean indicators of animals of all lines according to these traits was 10.7 days, or 5.6 % and 31 g, or 6.0 % respectively. The Zalon and Zenit line offsprings were inferior to the boars of the rest lines in terms of the growth in reaching a live weight of 100 kg by 2.8–19 days. Their indicators of the daily average growth from birth to a live weight of 100 kg also appeared to be, on the average, lower by 6–49 g. The differences in the Zaslon line boars were confident ( р ≤ 0.05).
{"title":"Productivity of pigs and the genealogical structure of the breeding herds of Belarusian meat breed","authors":"I. Sheyko, E. Yanovich, N. V. Pristupa, T. N. Timoshenko, R. Sheyko, I. V. Anihovskaya, K. A. Kаpshevich","doi":"10.29235/1561-8323-2020-64-2-245-256","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-2-245-256","url":null,"abstract":"The genealogical breed structure is represented by 5 main factory lines: Zubr, Zenit, Zaslon, Zont, Zefir. The genealogical structure of the Belarusian meat breed is formed using the generally accepted selection methods, including the assessment by pedigree, strict selection, targeted selection, the assessment of replacement young by productivity on elevers, the assessment of boars and queens by the offspring quality using the control feeding method. The targeted breeding work with herds of Belarusian meat breed pigs during a long period of time according to the long-term target program allows us to obtain large boars and queens that are characterized by the meat-type body. When assessing boar-producers of the Belarusian meat breed aged 36 months and older in all farms the values of the body live weight and length were on the average 305.3 kg and 179 cm respectively. As a result of studying the reproductive qualities of animals, it is established that, on the average, in the queens selected into the leading group in all farms, the indicators of prolificacy, the weight of one young pig at birth, the number of young pigs aged 21 days and milking ability were 11.74 animals and 1.11 kg, 10.06 animals and 54.34 respectively. The highest growth rate from birth to a live weight of 100 kg was seen in the Zefir line animals – 550 g. The indicator of the growth in reaching a live weight of 100 kg was 181 days. The superiority over the mean indicators of animals of all lines according to these traits was 10.7 days, or 5.6 % and 31 g, or 6.0 % respectively. The Zalon and Zenit line offsprings were inferior to the boars of the rest lines in terms of the growth in reaching a live weight of 100 kg by 2.8–19 days. Their indicators of the daily average growth from birth to a live weight of 100 kg also appeared to be, on the average, lower by 6–49 g. The differences in the Zaslon line boars were confident ( р ≤ 0.05).","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"66 1","pages":"245-256"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81563397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-20DOI: 10.29235/1561-8323-2020-64-1-71-77
I. Kazlouski, I. Belskaya, А. Zinchenko
Feasibility of producing brazzein plant sweetener by the cell-free biosynthesis procedure as an alternative to the classical submerged fermentation method was assessed in the present investigation. Chimeric RNA polymerase of T7 bacteriophage, S30-cell extract of Escherichia coli and multicopy plasmid vector pET42mut with the inserted brazzein gene were included into the protein synthesis. The completed research resulted in the first successful demonstration of the sweet protein biosynthesis in the cell-free system. The volumetric brazzein yield under optimized process conditions was 2 mg/ml of the reaction mixture, exceeding 57 times the maximum values that had been achieved in the previous studies applying whole-cell expression systems.
{"title":"Biosynthesis of brazzein using the bacterial cell-free protein synthesis system","authors":"I. Kazlouski, I. Belskaya, А. Zinchenko","doi":"10.29235/1561-8323-2020-64-1-71-77","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-1-71-77","url":null,"abstract":"Feasibility of producing brazzein plant sweetener by the cell-free biosynthesis procedure as an alternative to the classical submerged fermentation method was assessed in the present investigation. Chimeric RNA polymerase of T7 bacteriophage, S30-cell extract of Escherichia coli and multicopy plasmid vector pET42mut with the inserted brazzein gene were included into the protein synthesis. The completed research resulted in the first successful demonstration of the sweet protein biosynthesis in the cell-free system. The volumetric brazzein yield under optimized process conditions was 2 mg/ml of the reaction mixture, exceeding 57 times the maximum values that had been achieved in the previous studies applying whole-cell expression systems.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"107 1","pages":"71-77"},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79569787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-19DOI: 10.29235/1561-8323-2020-64-1-42-49
I. Matsukevich, A. Kulak, O. V. Polhovskaya, D. A. Kiiliomin
Anodic materials with spinel structure Li 2 MTi 3 O 8 (M - divalent metal) are promising for storing electricity from renewable energy sources, for portable electronics and electric vehicles. In this work, mesoporous lithium titanates Li 2 MTi 3 O 8 (M - Co, Cu, Zn) powders with spinel structure were prepared by the method of self-propagating high-temperature synthesis (SHS) from the glycine-citrate-nitrate mixtures and, for comparison, by the sol-gel method. Their crystal structure, phase composition, thermal stability, microstructure and dispersion were studied. It was established that the SHS method of preparation of lithium titanates has several advantages over the sol-gel method because there is no need to use any solvents; it is possible to reduce particle aggregation, to increase specific surface and to reduce bulk density of the obtained powders.
{"title":"Comparative analysis of the methods of producing lithium titanates","authors":"I. Matsukevich, A. Kulak, O. V. Polhovskaya, D. A. Kiiliomin","doi":"10.29235/1561-8323-2020-64-1-42-49","DOIUrl":"https://doi.org/10.29235/1561-8323-2020-64-1-42-49","url":null,"abstract":"Anodic materials with spinel structure Li 2 MTi 3 O 8 (M - divalent metal) are promising for storing electricity from renewable energy sources, for portable electronics and electric vehicles. In this work, mesoporous lithium titanates Li 2 MTi 3 O 8 (M - Co, Cu, Zn) powders with spinel structure were prepared by the method of self-propagating high-temperature synthesis (SHS) from the glycine-citrate-nitrate mixtures and, for comparison, by the sol-gel method. Their crystal structure, phase composition, thermal stability, microstructure and dispersion were studied. It was established that the SHS method of preparation of lithium titanates has several advantages over the sol-gel method because there is no need to use any solvents; it is possible to reduce particle aggregation, to increase specific surface and to reduce bulk density of the obtained powders.","PeriodicalId":11227,"journal":{"name":"Doklady Akademii nauk","volume":"90 1","pages":"42-49"},"PeriodicalIF":0.0,"publicationDate":"2020-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88779357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-07DOI: 10.29235/1561-8323-2019-63-6-747-754
S. Goncharov, A. E. Kozlov, M. V. Маtveyenkov, I. A. Cheshik
The reactivity of biological systems to UV-A/B and the photoprotective potential of skin applications of lichen extracts Hypogymnia physodes according to the biochemical parameters of blood and morphometric parameters of the skin were evaluated in vivo in laboratory mice. Ethanol, acetone and hexane–acetone extracts (1 % in dimethylsulfoxide) effectively absorb the UV range inducing the most erythema. On the 4th day after UV-A/Вirradiation, severe brown burns of the back skin, severe erythema and edema, scab formation were observed. In serum, there were significantly changed biochemical parameters – the prooxidant capacity, the level of nitrate/nitrite-ions NOx, advanced oxidation protein products AOPP, glutathioneperoxidase activity GPx increased, the level of SH-groups reduced. Solutions of extracts (5 %) in dimethylsulfoxide at a different degree exhibited the photoprotective effect: morphologically – in minimizing the burn symptoms of the skin (edema, erythema, scab); biochemically – in the regulation of these parameters at the control level. According to the set of biochemical and morphometric parameters, the most promising photoprotectors were acetone extracts. The prooxidant capacity, GPx, AOРР (in all cases reduced) and NOx showed a non-specificity and rather high sensitivity and are more suitable for estimating inflammatory and oxidative processes. The highest efficiency and the adequacy to the tasks and the specifications are shown by the level of protein SH-groups.
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