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Platelet gel and factors determining its biological activity 血小板凝胶及其生物活性的决定因素
Pub Date : 2024-01-07 DOI: 10.29235/15618323-2023-67-6-490-498
V. Y. Galitskaya, M. P. Potapnev, V. I. Asaevich, V. G. Bogdan, S. Kosmacheva, F. N. Karpenko
A platelet gel (PG) derived from platelet concentrate (PC) is considered as a perspective therapeutic agent with hemostatic and regenerative properties. PG was obtained from PC separated from human peripheral blood by automatic apheresis by adding human thrombin (30 U/ml). We compared the proliferation of human mesenchymal stromal cells (MSCs) in vitro in the presence of PG and the dependence of gel density on excess of fibrinogen, the presence of calcium chloride, calcium gluconate, and aprotinin. PG was formed from CT in the presence of thrombin during 5–10 minutes. PG as gel-like fibrin membrane contained platelets and an admixture of leukocytes, and was capable to enhance the proliferation of MSCs in vitro. The presence of calcium gluconate (10 mg/ml) increased in the presence of PG the rate of MSCs proliferation in vitro. The presence of aprotinin in PG at a concentration of 10–1000 KIU/ml caused a dose-dependent decrease in the rate of gel biodegradation and did not affect the ability of PG to stimulate the proliferation of human MSCs in vitro.
从血小板浓缩物(PC)中提取的血小板凝胶(PG)被认为是一种具有止血和再生特性的透视治疗剂。血小板凝胶是通过加入人凝血酶(30 U/ml)从人外周血中自动分离出来的。我们比较了人间质基质细胞(MSCs)在有 PG 存在时的体外增殖情况,以及凝胶密度对纤维蛋白原过量、氯化钙、葡萄糖酸钙和阿普罗宁存在的依赖性。在凝血酶的作用下,CT 在 5-10 分钟内形成 PG。作为凝胶状纤维蛋白膜的 PG 含有血小板和白细胞,能够促进间充质干细胞在体外的增殖。葡萄糖酸钙(10 毫克/毫升)的存在增加了间充质干细胞在体外的增殖速度。在浓度为 10-1000 KIU/ml 的 PG 中加入 aprotinin 会导致凝胶生物降解率呈剂量依赖性下降,但不会影响 PG 刺激人间叶干细胞体外增殖的能力。
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引用次数: 0
Modelling the redox imbalance and oxidative stress in the hippocampus at aluminum neurotoxicity and initiating the coenzyme А biosynthesis 模拟铝神经毒性时海马体内的氧化还原失衡和氧化应激,启动辅酶А的生物合成过程
Pub Date : 2024-01-07 DOI: 10.29235/1561-8323-2023-67-6-481-489
N. Kanunnikova, D. Semenovich, I. N. Katkovskaya, O. V. Titko, E. P. Lukiyenko, V. Gurinovich, A. Moiseenok
An Alzheimer-like pathological process was induced in mature female Wistar CRL: (WI) WUBR rats using aluminum chloride (200 mg/kg, intragastrically, 6 weeks) in order to model redox imbalance and oxidative stress (OS) in the hippocampus and study the possibilities of their correction 2 weekly administration of coenzyme A biosynthesis modulators (panthenol – PL, pantethine – PT, homopantothenate – HP) at a dose of 200 mg/kg intragastrically for 2 weeks). Against the background of activation of peroxidation processes and a decrease in acetylcholinesterase activity, a decrease in the reduction potential of glutathione and the level of the acid-soluble fraction of CoA was observed with a simultaneous increase in the activity of glutathione-metabolizing enzymes (GR, GPx, GST), the process of S-glutathionylation of proteins and the level of protein thiols. The consumption of the precursors of CoA biosynthesis in full (PL, PT) or in part (HP) had an antioxidant effect, restored the activity of AChE, the level and reduction potential of glutathione and glutathione-metabolizing enzymes, the process of S-glutathionylation, and stimulated the activity of enzymes generating NADPH+. Taking into account the low modulating effect of coenzyme precursors on the level of CoA in the hippocampus and their high redox pharmacological activity, their non-coenzymatic effect on redox mechanisms leading to an increase in the bioavailability of reducing equivalents and energy status is assumed.
使用氯化铝(200 毫克/千克,胃内注射,6 周)诱导成熟雌性 Wistar CRL:(WI) WUBR大鼠,以建立海马氧化还原失衡和氧化应激(OS)模型,并研究每周服用辅酶A生物合成调节剂(泛醇-PL、泛酸-PT、同泛酸盐-HP)的可能性。在过氧化过程被激活和乙酰胆碱酯酶活性降低的背景下,观察到谷胱甘肽的还原电位和 CoA 酸溶性部分的水平下降,同时谷胱甘肽代谢酶(GR、GPx、GST)的活性、蛋白质的 S-谷胱甘肽化过程和蛋白质硫醇的水平上升。完全(PL、PT)或部分(HP)摄入 CoA 生物合成的前体具有抗氧化作用,可恢复 AChE 的活性、谷胱甘肽和谷胱甘肽代谢酶的水平和还原电位、S-谷胱甘肽化过程,并刺激产生 NADPH+ 的酶的活性。考虑到辅酶前体对海马体内 CoA 水平的调节作用较低,而其氧化还原药理活性较高,因此假定其对氧化还原机制的非酶作用可导致还原当量生物利用率和能量状态的提高。
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引用次数: 0
Structure, soft magnetic properties of Fe-based magnetic composites and its practical application 铁基磁性复合材料的结构、软磁特性及其实际应用
Pub Date : 2024-01-07 DOI: 10.29235/1561-8323-2023-67-6-508-516
O. F. Demidenko, A. L. Zhaludkevich, A. O. Laryn, G. Govor, Sun Haibo, Dongchu Chen, S. Yankevich
A multi-stage technique for applying insulating coatings to metal powder particles has been developed in order to create a new class of Fe-based soft magnetic materials with improved characteristics. The density value calculated from the data of X-ray diffraction analysis is approximately 3 % higher than the experimentally measured values, which are 7.4–7.45 g/cm3. The low porosity of the composites is confirmed by the SEM and EDX results. The proposed method of encapsulation of iron powder with an oxide layer is a highly economical method for applying coatings of various chemical compositions to metal powders, and can be widely used in practice to obtain electrical materials. Comprehensive studies of the properties of the obtained samples of powder composite materials based on ABC100.30 iron, the particles of which are encapsulated with phosphorus oxide, have been carried out. It has been established that in a field of 1.5 T, the losses at a frequency of 1 kHz decrease 10 times with an increase in the thickness to 30 nm. The synthesized materials are recommended for use in the development of various types of high-frequency electric motors, generators, chokes, magnetic circuits and electrodes for high-frequency welding and other applications.
我们开发了一种在金属粉末颗粒上涂覆绝缘涂层的多阶段技术,以制造出一种具有更好特性的新型铁基软磁材料。根据 X 射线衍射分析数据计算得出的密度值比实验测量值(7.4-7.45 克/立方厘米)高出约 3%。扫描电子显微镜(SEM)和电离辐射 X 射线分析(EDX)结果证实了复合材料的低孔隙率。所提出的用氧化物层包裹铁粉的方法是一种非常经济的方法,可为金属粉末涂上各种化学成分的涂层,可广泛应用于获得电学材料。我们对基于 ABC100.30 铁(其颗粒被氧化磷包裹)的粉末复合材料样品的性能进行了全面研究。结果表明,在 1.5 T 的电场中,厚度增加到 30 nm 时,频率为 1 kHz 时的损耗降低了 10 倍。建议将合成材料用于开发各类高频电机、发电机、扼流圈、磁路和高频焊接电极及其他应用。
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引用次数: 0
Spatial and temporal coherence of quasi-periodic components of meteorological fields as a basis for long-term weather forecasts 作为长期天气预报基础的气象场准周期成分的空间和时间一致性
Pub Date : 2024-01-07 DOI: 10.29235/1561-8323-2023-67-6-499-507
S. A. Lysenko, V. F. Loginov
A new method of teleconnections studding is proposed which is based on the identification of conjugate regions in the global meteorological fields of temperature and pressure by their characteristic coherent quasi-periodic oscillation. This method was implemented in order to select predictors of winter air temperature in Belarus with an advance of 2 months. The degree of coherence of sea level pressure and winter temperature in Belarus on a quasi-8-year cycle was considered as a criterion for the selection of predictors. The forecast was implemented using the advanced deep machine learning model TimesNet and showed rather high metrics of quality for seasonal meteorological forecasting: the correlation coefficient between actual and predicted temperature values was 0.66, and the weighted macro-average values of precision and recall of the forecast in the gradations “normal”, “above normal” and “below normal” were 0.61 and 0.56, respectively.
提出了一种新的远程连接研究方法,其基础是根据全球气象领域中温度和压力的共轭区域的相干准周期振荡特征进行识别。采用这种方法是为了选择白俄罗斯冬季气温的预报因子,提前量为 2 个月。白俄罗斯海平面气压和冬季气温在准 8 年周期内的一致性程度被视为选择预测因子的标准。使用先进的深度机器学习模型 TimesNet 进行了预测,结果显示季节性气象预报的质量指标相当高:实际气温值和预测气温值之间的相关系数为 0.66,在 "正常"、"高于正常 "和 "低于正常 "等级中,预测的精确度和召回率的加权宏观平均值分别为 0.61 和 0.56。
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引用次数: 0
Effect of a nonionic surfactant and anionic polyelectrolyte binary system on the stability of a calcium and magnesium carbonates dispersion 非离子表面活性剂和阴离子聚电解质二元体系对碳酸钙和碳酸镁分散体稳定性的影响
Pub Date : 2024-01-06 DOI: 10.29235/1561-8323-2023-67-6-473-480
S. V. Bucha, A. D. Vorobiev, D. V. Cherednichenko, E. V. Vorobieva, E. V. Laevskaya, N. Krutko, H. V. Bondareva
The effect of sodium polyacrylate, polyethylene glycol of various molecular weights, and their mixtures on the dispersion stability of calcium and magnesium carbonates was studied. It was shown that in the presence of a binary system, the light transmission of the dispersion decreases 1.2–1.5 times, the content of fine fraction particles increases by 1.1–1.3 times compared to the individual components. The stability of the dispersion of carbonates depends on the components ratio in the binary system and their molecular weight. The formation of adsorption solvated layers of polyacrylate and polyethylene glycol molecules at the interphase boundary contributes to an increase in the stability of the carbonate dispersion.
研究了聚丙烯酸钠、不同分子量的聚乙二醇及其混合物对碳酸钙和碳酸镁分散稳定性的影响。结果表明,在二元体系存在的情况下,分散体的透光率比单独成分降低 1.2-1.5 倍,细小颗粒的含量增加 1.1-1.3 倍。碳酸盐分散体的稳定性取决于二元体系中各组分的比例及其分子量。聚丙烯酸酯和聚乙二醇分子在相间边界形成的吸附溶解层有助于提高碳酸盐分散体的稳定性。
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引用次数: 0
Abnormal swelling in water of carboxylic ion exchanger in the forms of ammonium and tetraethylammonium ions 铵离子和四乙基铵离子形式的羧基离子交换剂在水中的异常膨胀
Pub Date : 2024-01-06 DOI: 10.29235/1561-8323-2023-67-6-465-472
V. S. Soldatov, E. Kosandrovich, T. V. Bezyazychnaya
A fibrous carboxylic ion exchanger, obtained by post-radiation grafting of polyacrylic acid to polypropylene fiber, in the ammonium form has an abnormally low swelling in water, corresponding to 10 moles of water per equivalent, and in the form of tetraethylammonium ion (NEt4+) – abnormally high 25 moles of water/eq. Considering that NH4+ is a hydrophilic particle and NEt4+ is hydrophobic, this fact seems paradoxical. The article attempts to explain this phenomenon using molecular modeling (ab initio calculations of the structure of hydrate complexes in the HF MO LCAO approximation with the 6-31G basis set) in combination with the Predominant Hydrates Model, which made it possible to calculate the theoretical water sorption isotherms of the ion exchanger and compare them with experimental data. The abnormally low swelling of the ion exchanger in the NH4+-form is caused by the formation of a strong bond between the carboxylate anion and ammonium with a significant degree of covalence due to the superposition of the Coulomb interaction of the ions and the formation of a hydrogen bond between them. The abnormally high swelling of NEt4+-forms is caused by the absence of a strong interaction between cations and carboxylate groups due to the impossibility of their convergence due to steric reasons. The high swelling of the ion exchanger is caused by the absence of blocking of the hydrophilic carboxylate groups of the ion exchanger by hydrogen bonds with the cation. The ion exchanger matrix does not contain a cross agent and does not create a spatial restriction for the high swelling of the ion exchanger.
在聚丙烯纤维上通过辐照后接枝聚丙烯酸而获得的纤维状羧酸离子交换剂,以铵形式存在时,在水中的膨胀率异常低,相当于 10 摩尔水/当量,而以四乙基铵离子(NEt4+)形式存在时,膨胀率异常高,达到 25 摩尔水/当量。考虑到 NH4+ 是亲水性粒子,而 NEt4+ 是疏水性粒子,这一事实似乎自相矛盾。文章试图利用分子建模(在 HF MO LCAO 近似中利用 6-31G 基集对水合物复合物的结构进行 ab initio 计算)并结合 Predominant Hydrates Model(主要水合物模型)来解释这一现象,从而计算出离子交换器的理论吸水等温线,并将其与实验数据进行比较。NH4+ 形式的离子交换剂膨胀率异常低的原因是羧酸根阴离子和铵之间形成了强键,由于离子之间的库仑相互作用和氢键形成的叠加作用,羧酸根阴离子和铵之间形成了共价程度很高的强键。NEt4+ 形式的膨胀率异常之高,是因为阳离子和羧基之间没有强烈的相互作用,这是因为立体原因导致阳离子和羧基不可能聚合在一起。离子交换剂的高膨胀性是由于离子交换剂的亲水性羧酸基团与阳离子之间没有氢键阻隔。离子交换剂基体不含交叉剂,不会对离子交换剂的高膨胀性造成空间限制。
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引用次数: 0
On some classes of finite σ-soluble PσT-groups 关于有限σ可溶PσT群的一些类别
Pub Date : 2024-01-06 DOI: 10.29235/1561-83232023-67-6-460-464
I. N. Safonova, A. Skiba
Let X be a class of groups. Suppose that with each group G ∈ X we associate some system of its subgroups τ(G). Then τ is said to be a subgroup functor on X if the following conditions are hold: (1) G ∈  τ(G) for each group G ∈ X; (2) for any epimorphism φ: A → B, where A, B ∈ X, and for any groups H ∈ τ(A) and T ∈ τ(B) we have Hφ ∈ τ(B) and Tφ-1 ∈ τ( A). In this paper, were considered some applications of such subgroup functors in the theory of finite groups in which generalized normality for subgroups is transitive.
让 X 是一类群。假设每个群 G∈X 都与它的子群 τ(G) 关联。那么,如果以下条件成立,我们就说 τ 是 X 上的一个子群函子:(1)对于每个群 G∈X,G∈τ(G);(2)对于任何外形变 φ:A→B,其中 A,B∈X,对于任何群 H∈τ(A) 和 T∈τ(B) 我们有 Hφ∈τ(B) 和 Tφ-1∈τ( A)。本文考虑了这种子群函数在有限群理论中的一些应用,在有限群理论中,子群的广义正则性是反式的。
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引用次数: 0
Unconditionally monotone and globally stable difference schemes for the Fisher equation 费雪方程的无条件单调和全局稳定差分方案
Pub Date : 2024-01-05 DOI: 10.29235/1561-8323-2023-67-6-454-459
P. P. Matus, D. Pylak
In this paper, we construct and study unconditionally monotone and globally stable difference schemes for the Fisher equation. It has been shown that constructed schemes inherit the stability property of the exact solution: 0 ≤ u(x, t) ≤ 1, (x, t) ∈ QT = {(x, t) : 0 ≤ x ≤ l, 0 ≤ t < +∞} for a given input data of the problem. The unconditional monotonicity of the difference schemes is proved and the a priori estimate is obtained in the uniform norm for the difference solution. The stable behavior of the difference solution in the nonlinear case takes place under slightly more stringent constraints on the input data: 0,5 ≤ u0 (x), µ1(t), µ2(t) ≤ 1.
本文构建并研究了费雪方程的无条件单调全局稳定差分方案。研究表明,所构建的方案继承了精确解的稳定性:0 ≤ u(x, t) ≤ 1, (x, t) ∈ QT = {(x, t) :0 ≤ x ≤ l, 0 ≤ t < +∞} 对于问题的给定输入数据。证明了差分方案的无条件单调性,并在差分解的统一规范中获得了先验估计。在非线性情况下,差分解的稳定行为发生在对输入数据略微严格的约束条件下:0,5 ≤ u0 (x), µ1(t), µ2(t) ≤ 1。
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引用次数: 0
Belarusian humanitarian knowledge in the XXI century 二十一世纪白俄罗斯的人道主义知识
Pub Date : 2024-01-05 DOI: 10.29235/1561-8323-2023-67-6-447-453
A. A. Kavalenia
The article shows the achievements of the Department of Humanitarian Sciences and Arts of the National Academy of Sciences of Belarus at the beginning of the 21st century.
文章展示了白俄罗斯国家科学院人道主义科学与艺术部在21世纪初取得的成就。
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引用次数: 0
Assessing the production processes on the most typical farms and milk production complexes 评估最典型的牧场和牛奶生产联合企业的生产流程
Pub Date : 2024-01-05 DOI: 10.29235/1561-8323-2023-67-6-517-528
P. Kazakevich, U. N. Tsimoshanka, A. A. Muzyka, S. A. Kirikovich, L. N. Sheigratsova, M. P. Puchka, N. N. Shmatko, M. V. Tsimoshanka, A. I. Konek
The article presents the results of research, according to an energy assessment, of the most common options for combining spatial planning and technological solutions of buildings and structures, the set of technological equipment used on the most typical operating farms and milk production complexes. The most significant elements of technology and technological equipment, its operating modes, energy consumption by cost elements for the processes of preparation and distribution of feed, manure removal and provision of the required microclimate parameters, determining the intensity level of the production process in various variants of building layout and livestock, have been studied. The results obtained allow us to determine the most effective options for mechanization and automation of the production process and improve the technological methods of their implementation.
文章介绍了根据能源评估结果,对建筑和结构的空间规划与技术解决方案相结合的最常见方案,以及最典型的经营性牧场和牛奶生产综合体所使用的成套技术设备进行研究的结果。研究了最重要的技术和技术设备要素、其运行模式、饲料制备和分配过程中的成本要素能耗、粪便清除和所需微气候参数的提供,确定了各种建筑布局和牲畜生产过程的强度水平。根据研究结果,我们可以确定生产过程机械化和自动化的最有效方案,并改进实施这些方案的技术方法。
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引用次数: 0
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Doklady of the National Academy of Sciences of Belarus
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