Pub Date : 2021-06-20DOI: 10.3329/dujps.v19i2.50856
A. Rahman, R. Hossain, Aslam Hossain, Shah Amran
Bangladesh approved the proposal for a National Drug Policy on May 29, 1982. We know that such drug policies are developed gradually over a period of time and may contain a lot of comprehensive documents. But in Bangladesh, the expert committee worked out the policy, based on 16 standards within 15 days. This vital document, almost unchanged, was made a law on 12 June 1982. A few years later, it can be observed that despite opposition from many concerns, the output of essential drugs has increased from about 30 to about 80 percent, prices have in almost all cases gone down considerably, the domestic industry has grown rapidly, the quality of its production has increased dramatically, and people’s awareness about quality medicines has been steadily growing. The World Health Organization (WHO) has stressed the need of a formulated drug policy in every country of the world in 1986. Bangladesh responded very early to this respect. Subsequently, two more national drug policies were promulgated in 2005 and 2016 respectively. Experience over the decades has shown that the said policies could not fulfill the declared objective of ensuring health for all. Our aim is to describe some of the lacunae for which total implementation of drug policy is still struggling. To find the root causes, a total of five hundred volunteers were surveyed by supplying a questionnaire on drug policy. It was observed that most of the participants opined that the incumbent government needs to be more stringent to implement the drug policy into reality by utilizing the public servants and public sectors, especially health personnel to ensure health for all. Dhaka Univ. J. Pharm. Sci. 20(1): 41-48, 2021 (June)
{"title":"A Study on the National Drug Policies of Bangladesh to Ensure Health for All","authors":"A. Rahman, R. Hossain, Aslam Hossain, Shah Amran","doi":"10.3329/dujps.v19i2.50856","DOIUrl":"https://doi.org/10.3329/dujps.v19i2.50856","url":null,"abstract":"Bangladesh approved the proposal for a National Drug Policy on May 29, 1982. We know that such drug policies are developed gradually over a period of time and may contain a lot of comprehensive documents. But in Bangladesh, the expert committee worked out the policy, based on 16 standards within 15 days. This vital document, almost unchanged, was made a law on 12 June 1982. A few years later, it can be observed that despite opposition from many concerns, the output of essential drugs has increased from about 30 to about 80 percent, prices have in almost all cases gone down considerably, the domestic industry has grown rapidly, the quality of its production has increased dramatically, and people’s awareness about quality medicines has been steadily growing. The World Health Organization (WHO) has stressed the need of a formulated drug policy in every country of the world in 1986. Bangladesh responded very early to this respect. Subsequently, two more national drug policies were promulgated in 2005 and 2016 respectively. Experience over the decades has shown that the said policies could not fulfill the declared objective of ensuring health for all. Our aim is to describe some of the lacunae for which total implementation of drug policy is still struggling. To find the root causes, a total of five hundred volunteers were surveyed by supplying a questionnaire on drug policy. It was observed that most of the participants opined that the incumbent government needs to be more stringent to implement the drug policy into reality by utilizing the public servants and public sectors, especially health personnel to ensure health for all. \u0000Dhaka Univ. J. Pharm. Sci. 20(1): 41-48, 2021 (June)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81409597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-20DOI: 10.3329/dujps.v19i2.50852
Shadhan Kumar Mondal, S. Chowdhury, A. Ganguly, A. Faroque
Pharmaceutical sector of Bangladesh has developed profoundly after promulgation of the Drugs (Control) Ordinance, 1982. However, the health sector has not been equally developed because of lack of wellequipped drug management system and much needed patient counseling. The presence of adulterated, counterfeit and substandard drugs and the sale of drugs at high prices than the maximum retail price have also been the major problems here. The recent introduction of model pharmacies is supposed to be a hope for the people to get safe medicines at a reasonable cost. The aim of the present study was to find out the current scenario of model pharmacies in Bangladesh and to propose modern and alternative systems that could be applied in model pharmacies for better healthcare management and patient compliance. Thus, the current status of model pharmacies of Bangladesh has been evaluated using a survey-based analysis which utilized a pre-set questionnaire. The survey was conducted on 90 model pharmacies in seven districts of Bangladesh (Level 1 categorized by the Directorate General of Drug Administration, Government of the People’s Republic of Bangladesh). The results revealed that the infrastructure of the model pharmacies should be improved further. Only 33% of the model pharmacies have sitting facilities and 51% of them have washroom facilities for the waiting patients. It was found that despite all the model pharmacies (100%) should have at least 1 A-grade pharmacist in each of them, i.e. a pharmacy graduate registered with the Pharmacy Council of Bangladesh under the Pharmacy Ordinance 1976, but pharmacists were found to be present in only 26% of pharmacies during the visit. Amongst the pharmacists, 98% showed satisfaction with the decision of compulsory engagement of A-grade pharmacists in all the model pharmacies. Defying the obligatory provisions, only 38% model of pharmacies keep the required records of sold drugs. It was pleasing to observe that no physician’s sample of medicines was sold in any model pharmacies. The medicines storage facilities in controlled temperature was found in all the model pharmacies. But the A-grade pharmacists were not available in the pharmacies during holidays. It is opined that modern and ICT based techniques can be applied to modify the model pharmacies for better patient care and patient management. Dhaka Univ. J. Pharm. Sci. 20(1): 1-10, 2021 (June)
{"title":"Evaluation of Current Status of Newly Established Model Pharmacies in Bangladesh","authors":"Shadhan Kumar Mondal, S. Chowdhury, A. Ganguly, A. Faroque","doi":"10.3329/dujps.v19i2.50852","DOIUrl":"https://doi.org/10.3329/dujps.v19i2.50852","url":null,"abstract":"Pharmaceutical sector of Bangladesh has developed profoundly after promulgation of the Drugs (Control) Ordinance, 1982. However, the health sector has not been equally developed because of lack of wellequipped drug management system and much needed patient counseling. The presence of adulterated, counterfeit and substandard drugs and the sale of drugs at high prices than the maximum retail price have also been the major problems here. The recent introduction of model pharmacies is supposed to be a hope for the people to get safe medicines at a reasonable cost. The aim of the present study was to find out the current scenario of model pharmacies in Bangladesh and to propose modern and alternative systems that could be applied in model pharmacies for better healthcare management and patient compliance. Thus, the current status of model pharmacies of Bangladesh has been evaluated using a survey-based analysis which utilized a pre-set questionnaire. The survey was conducted on 90 model pharmacies in seven districts of Bangladesh (Level 1 categorized by the Directorate General of Drug Administration, Government of the People’s Republic of Bangladesh). The results revealed that the infrastructure of the model pharmacies should be improved further. Only 33% of the model pharmacies have sitting facilities and 51% of them have washroom facilities for the waiting patients. It was found that despite all the model pharmacies (100%) should have at least 1 A-grade pharmacist in each of them, i.e. a pharmacy graduate registered with the Pharmacy Council of Bangladesh under the Pharmacy Ordinance 1976, but pharmacists were found to be present in only 26% of pharmacies during the visit. Amongst the pharmacists, 98% showed satisfaction with the decision of compulsory engagement of A-grade pharmacists in all the model pharmacies. Defying the obligatory provisions, only 38% model of pharmacies keep the required records of sold drugs. It was pleasing to observe that no physician’s sample of medicines was sold in any model pharmacies. The medicines storage facilities in controlled temperature was found in all the model pharmacies. But the A-grade pharmacists were not available in the pharmacies during holidays. It is opined that modern and ICT based techniques can be applied to modify the model pharmacies for better patient care and patient management. \u0000Dhaka Univ. J. Pharm. Sci. 20(1): 1-10, 2021 (June)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78003304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-14DOI: 10.3329/dujps.v20i1.54036
N. Ahsan, Monzilur Rahman, N. Islam, A. Akhand
Multidrug-resistant (MDR) organisms are spreading widely and becoming an issue of utmost importance to deal with. In the current study, ten urine samples from diabetic patients suffering from multiple complications, including urinary tract infection (UTI) and nephropathy were investigated. Antibiogram assays of the bacterial isolates from collected samples demonstrated resistance against most of the antibiotics tested. Further studies were conducted to determine the types of resistant bacteria that caused UTI. Analyzing the 16S rDNA sequence and phylogenetic tree, 3 isolates were identified as Escherichia coli, 5 as Klebsiella pneumoniae and the rest 2 as Enterobacter asburiae. The findings of this research indicate the necessity of urgent attention to find an effective alternative drug for treating infections caused by these resistant isolates. Dhaka Univ. J. Pharm. Sci. 20(1): 87-93, 2021 (June)
{"title":"Isolation and Characterization of Multidrug Resistant Enterobacteriaceae in Urine Sample of Patients Suffering from Urinary Tract Infection with Diabetes and Nephropathy","authors":"N. Ahsan, Monzilur Rahman, N. Islam, A. Akhand","doi":"10.3329/dujps.v20i1.54036","DOIUrl":"https://doi.org/10.3329/dujps.v20i1.54036","url":null,"abstract":"Multidrug-resistant (MDR) organisms are spreading widely and becoming an issue of utmost importance to deal with. In the current study, ten urine samples from diabetic patients suffering from multiple complications, including urinary tract infection (UTI) and nephropathy were investigated. Antibiogram assays of the bacterial isolates from collected samples demonstrated resistance against most of the antibiotics tested. Further studies were conducted to determine the types of resistant bacteria that caused UTI. Analyzing the 16S rDNA sequence and phylogenetic tree, 3 isolates were identified as Escherichia coli, 5 as Klebsiella pneumoniae and the rest 2 as Enterobacter asburiae. The findings of this research indicate the necessity of urgent attention to find an effective alternative drug for treating infections caused by these resistant isolates. \u0000Dhaka Univ. J. Pharm. Sci. 20(1): 87-93, 2021 (June)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77843320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-14DOI: 10.3329/dujps.v20i1.54040
M. Islam, M. Alam, K. Sikdar, Asm Monjur Al Hossain, A. S. Rouf
Worldwide more than 180 million people get infected by chronic Hepatitis C Virus (HCV) and around 700,000 people pass away every year due to HCV related problems. Sofosbuvir and Ribavirin are prescribed as combined medication for the management of HCV infection globally. Therefore, optimization of a pharmaceutical dosage form containing Sofosbuvir and Ribavirin can open a new hope in the treatment of HCV infection. This study was conducted by using the Design of Experiments (DoE) approach. It is a systematic process aiming to determine the correlation between formulation factors affecting the approach and the output of the approach. During the development of formulations, two factors were considered, which are Croscarmellose Sodium (CCS) and Povidone K30 (PK30) at different concentrations ranging from 0.19% to 2.31% of the total tablet weight in 9 different formulations. Their effect on disintegration time (DT) was evaluated through statistical method and it was found between 3±0.003 and 6.5±0.015 minutes. The use of these two different disintegrants exhibited a significant effect on DT. The contour plot showed predicted ranges of concentrations of CCS and PK30 for desired DT. Fourier Transform Infrared Spectroscopy (FTIR) data, Thermogravimetric Analysis (TGA) and X-ray Diffractometry (XRD) spectrums confirmed that there was no interaction between Sofosbuvir and Ribavirin or with any other excipients used in this experiment. Dhaka Univ. J. Pharm. Sci. 20(1): 121-133, 2021 (June)
{"title":"Development and Characterization of a Combination Tablet Dosage Form Containing Sofosbuvir and Ribavirin Using Design of Experiments (DoE) Approach","authors":"M. Islam, M. Alam, K. Sikdar, Asm Monjur Al Hossain, A. S. Rouf","doi":"10.3329/dujps.v20i1.54040","DOIUrl":"https://doi.org/10.3329/dujps.v20i1.54040","url":null,"abstract":"Worldwide more than 180 million people get infected by chronic Hepatitis C Virus (HCV) and around 700,000 people pass away every year due to HCV related problems. Sofosbuvir and Ribavirin are prescribed as combined medication for the management of HCV infection globally. Therefore, optimization of a pharmaceutical dosage form containing Sofosbuvir and Ribavirin can open a new hope in the treatment of HCV infection. This study was conducted by using the Design of Experiments (DoE) approach. It is a systematic process aiming to determine the correlation between formulation factors affecting the approach and the output of the approach. During the development of formulations, two factors were considered, which are Croscarmellose Sodium (CCS) and Povidone K30 (PK30) at different concentrations ranging from 0.19% to 2.31% of the total tablet weight in 9 different formulations. Their effect on disintegration time (DT) was evaluated through statistical method and it was found between 3±0.003 and 6.5±0.015 minutes. The use of these two different disintegrants exhibited a significant effect on DT. The contour plot showed predicted ranges of concentrations of CCS and PK30 for desired DT. Fourier Transform Infrared Spectroscopy (FTIR) data, Thermogravimetric Analysis (TGA) and X-ray Diffractometry (XRD) spectrums confirmed that there was no interaction between Sofosbuvir and Ribavirin or with any other excipients used in this experiment. \u0000Dhaka Univ. J. Pharm. Sci. 20(1): 121-133, 2021 (June)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87269899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-10DOI: 10.3329/dujps.v19i2.50636
C. Sultana, A. Azad, Mominur Rahman, Abdul Muhit, S. Rahman
Three fractions obtained by Kupchan partitioning of 70% methanol extract of leaves of Stevia rebaudiana with n-hexane, dichloromethane and ethyl acetate designated by HSR, DSR and ESR were investigated for their antioxidant and antimicrobial activities. HSR and ESR exhibited prominent free radical scavenging activity having IC50 value of 11.59 and 10.38 μg/ml compared to standard, ascorbic acid (IC50 value 6.05 μg/ml). HSR and ESR also demonstrated significant antibacterial activity by disc diffusion method on some Gram-positive and Gramnegative bacteria. Among the fractions ESR showed the highest antioxidant and antibacterial activity. No significant antifungal activity was observed for any fraction. Preliminary phytochemical screening of all fractions showed the presence of alkaloids, saponin, flavonoids, tannin, carbohydrate and triterpenoids. Total three compounds have been isolated from ESR fraction among which two known compounds (1-2) were characterized as quercitrin (1) and physcion (2) through 1D NMR spectroscopic technique and other one could be a 9,10- anthraquinone derivative which was not characterized yet. The compound physcion (2) is first time report from the plant Stevia rebaudiana. Dhaka Univ. J. Pharm. Sci. 19(2): 191-197, 2020 (December)
{"title":"Phytochemical and Biological Investigation of Stevia rebaudiana (Bert.) Leaves Grown in Bangladesh","authors":"C. Sultana, A. Azad, Mominur Rahman, Abdul Muhit, S. Rahman","doi":"10.3329/dujps.v19i2.50636","DOIUrl":"https://doi.org/10.3329/dujps.v19i2.50636","url":null,"abstract":"Three fractions obtained by Kupchan partitioning of 70% methanol extract of leaves of Stevia rebaudiana with n-hexane, dichloromethane and ethyl acetate designated by HSR, DSR and ESR were investigated for their antioxidant and antimicrobial activities. HSR and ESR exhibited prominent free radical scavenging activity having IC50 value of 11.59 and 10.38 μg/ml compared to standard, ascorbic acid (IC50 value 6.05 μg/ml). HSR and ESR also demonstrated significant antibacterial activity by disc diffusion method on some Gram-positive and Gramnegative bacteria. Among the fractions ESR showed the highest antioxidant and antibacterial activity. No significant antifungal activity was observed for any fraction. Preliminary phytochemical screening of all fractions showed the presence of alkaloids, saponin, flavonoids, tannin, carbohydrate and triterpenoids. Total three compounds have been isolated from ESR fraction among which two known compounds (1-2) were characterized as quercitrin (1) and physcion (2) through 1D NMR spectroscopic technique and other one could be a 9,10- anthraquinone derivative which was not characterized yet. The compound physcion (2) is first time report from the plant Stevia rebaudiana. \u0000Dhaka Univ. J. Pharm. Sci. 19(2): 191-197, 2020 (December)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81189081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-10DOI: 10.3329/dujps.v19i2.50634
B. Revanasiddappa, M. Kumar, H. Kumar
In the present study a series of substituted pyrazolines (3a-f) has been synthesized by reacting chalcones (2a-f) and semicarbazide (1) in methanol medium. All the title compounds were assessed for their in-vivo anti-depressant activity by tail suspension test (TST) and forced swimming test (FST) methods. Compound 3a was found to exhibit moderate antidepressant activity in comparison to standard Imipramine. Newly synthesized compounds were characterized by mass (MS), 1H-NMR and infrared (IR) spectral analytical data. Dhaka Univ. J. Pharm. Sci. 19(2): 179-184, 2020 (December)
{"title":"Synthesis and Antidepressant Activity of Pyrazoline Derivatives","authors":"B. Revanasiddappa, M. Kumar, H. Kumar","doi":"10.3329/dujps.v19i2.50634","DOIUrl":"https://doi.org/10.3329/dujps.v19i2.50634","url":null,"abstract":"In the present study a series of substituted pyrazolines (3a-f) has been synthesized by reacting chalcones (2a-f) and semicarbazide (1) in methanol medium. All the title compounds were assessed for their in-vivo anti-depressant activity by tail suspension test (TST) and forced swimming test (FST) methods. Compound 3a was found to exhibit moderate antidepressant activity in comparison to standard Imipramine. Newly synthesized compounds were characterized by mass (MS), 1H-NMR and infrared (IR) spectral analytical data. \u0000Dhaka Univ. J. Pharm. Sci. 19(2): 179-184, 2020 (December)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81725081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-10DOI: 10.3329/dujps.v19i2.50633
D. Prasanthi, N. Priya, Amoolya Chennuri, P. Lakshmi
Emulsifier-free, particle-stabilised emulsions called Pickering emulsions of fluconazole for topical delivery, were studied using Taguchi L9 orthogonal array (OA) design. Formulations were prepared by homogenization method, using bentonite in the concentration range of 2-4% w/v, oil phase (coconut oil, oleic acid, sesame oil). In Taguchi L9 OA experimental design, independent variables (oil phase & concentration of bentonite) effect on dependent variables (drug content & release rate) was studied. All the formulations have shown good physicochemical properties. The PS3 formulation containing sesame oil and 4% bentonite was optimized as particle size was found to be 758.0 nm, zeta potential -48 mv, with Q8 of 166.70±0.15μg/cm2, flux of 17.33±0.02 μg/cm2/hr, permeability coefficient of 3.46±0.015 cm/hr×10-3 and skin content of 4.01±0.24 mg/g. The formulations were stable and non-irritant. Hence pickering emulsion of fluconazole using sesame oil can be used for topical delivery as the drug release was maintained at the localised site. Dhaka Univ. J. Pharm. Sci. 19(2): 169-178, 2020 (December)
{"title":"Optimization of Fluconazole Pickering Emulsion Using Taguchi Orthogonal Array Design","authors":"D. Prasanthi, N. Priya, Amoolya Chennuri, P. Lakshmi","doi":"10.3329/dujps.v19i2.50633","DOIUrl":"https://doi.org/10.3329/dujps.v19i2.50633","url":null,"abstract":"Emulsifier-free, particle-stabilised emulsions called Pickering emulsions of fluconazole for topical delivery, were studied using Taguchi L9 orthogonal array (OA) design. Formulations were prepared by homogenization method, using bentonite in the concentration range of 2-4% w/v, oil phase (coconut oil, oleic acid, sesame oil). In Taguchi L9 OA experimental design, independent variables (oil phase & concentration of bentonite) effect on dependent variables (drug content & release rate) was studied. All the formulations have shown good physicochemical properties. The PS3 formulation containing sesame oil and 4% bentonite was optimized as particle size was found to be 758.0 nm, zeta potential -48 mv, with Q8 of 166.70±0.15μg/cm2, flux of 17.33±0.02 μg/cm2/hr, permeability coefficient of 3.46±0.015 cm/hr×10-3 and skin content of 4.01±0.24 mg/g. The formulations were stable and non-irritant. Hence pickering emulsion of fluconazole using sesame oil can be used for topical delivery as the drug release was maintained at the localised site. \u0000Dhaka Univ. J. Pharm. Sci. 19(2): 169-178, 2020 (December)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91218624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-10DOI: 10.3329/dujps.v19i2.50629
Kazi Ruhullah Shahriar, Sirajis Salekin, Q. S. Islam, M. Ahsan, C. M. Hasan
The ethyl acetate extract of bark of Aporosa wallichii Hook.f. was extensively investigated for the chemical characterization of secondary metabolites and for biological activities. After repeated chromatographic separation and purification, two compounds were elucidated as a phenolic compound ferulic acid (1) and an uncommon triterpene glut-5(6)-en-3β-ol (2) from the ethyl acetate extract of barks of A. wallichii by using high field NMR analyses. This is the first report of isolation of both compounds from this plant species. No significant antimicrobial activity was observed for any fraction after Kupchan partitioning of the extract. During the antioxidant activity assay, the ethyl acetate soluble fraction of A. wallichii demonstrated significant DPPH radical scavenging capacity with an IC50 value of 1.25 μg/mL. The other fractions viz. petroleum ether, aqueous and chloroform soluble fractions exhibited moderate, mild, and weak activity respectively. The petroleum ether soluble fraction demonstrated maximum thrombolytic property (51.33%) which was similar to that of standard streptokinase (66.81%). Dhaka Univ. J. Pharm. Sci. 19(2): 139-143, 2020 (December)
{"title":"Phytochemical and Biological Studies on Bark Extract of Aporosa wallichii Hook.f.","authors":"Kazi Ruhullah Shahriar, Sirajis Salekin, Q. S. Islam, M. Ahsan, C. M. Hasan","doi":"10.3329/dujps.v19i2.50629","DOIUrl":"https://doi.org/10.3329/dujps.v19i2.50629","url":null,"abstract":"The ethyl acetate extract of bark of Aporosa wallichii Hook.f. was extensively investigated for the chemical characterization of secondary metabolites and for biological activities. After repeated chromatographic separation and purification, two compounds were elucidated as a phenolic compound ferulic acid (1) and an uncommon triterpene glut-5(6)-en-3β-ol (2) from the ethyl acetate extract of barks of A. wallichii by using high field NMR analyses. This is the first report of isolation of both compounds from this plant species. No significant antimicrobial activity was observed for any fraction after Kupchan partitioning of the extract. During the antioxidant activity assay, the ethyl acetate soluble fraction of A. wallichii demonstrated significant DPPH radical scavenging capacity with an IC50 value of 1.25 μg/mL. The other fractions viz. petroleum ether, aqueous and chloroform soluble fractions exhibited moderate, mild, and weak activity respectively. The petroleum ether soluble fraction demonstrated maximum thrombolytic property (51.33%) which was similar to that of standard streptokinase (66.81%). \u0000Dhaka Univ. J. Pharm. Sci. 19(2): 139-143, 2020 (December)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89526253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-10DOI: 10.3329/dujps.v19i2.50630
L. Gadouche, A. Saadi, K. Zerrouki, N. Djebli, Meryem Sendjasni, Yasmina Djouder, Fatima Zohra Benali Fellague, Kheira Benouadah
Free radicals, oxidative stress or antioxidants is more and more often used to explain different pathological disorders and their therapeutic approach. The aim of this study was to evaluate the protective effect of anthocyanin extract obtained by maceration of the flowers of Lavandula dentata in 0.1 % HCl/methanol (v/v) solution on oxidative stress. The antioxidant activity of anthocyanin extract in vitro was evaluated by reduction of iron (FRAP), DPPH and the s-carotene tests. The in vivo oxidative stress was induced by intraperitoneal injection of 0.5 ml/kg of CCl4 and treated orally by 500mg kg/day of the extract. Anthocyanins extract inhibited the free radical DPPH (IC50:1.3 ± 0.23 mg/ml). Lavender extract prevented the oxidation of B carotene (28.34 ± 0.07%) and has an ability to reduce iron (0.736 ± 0.03). Intraperitoneal injection of CCl4 has increased biochemical parameters, which was evidence of oxidative stress in vivo. In contrast, daily oral administration of anthocyanin extract has restored the biochemical parameters. Histopathological examinations of liver stained with haematoxylin and eosin showed loss of hepatic architecture. These injuries observed have been improved by treatment with anthocyanin extract. The findings revealed that anthocyanin extract from lavender possesses a significant antioxidant activity. Dhaka Univ. J. Pharm. Sci. 19(2): 145-151, 2020 (December)
{"title":"Anthocyanin Extract from Flowers of Lavender dentate Beats Oxidative Stress In vitro and In vivo","authors":"L. Gadouche, A. Saadi, K. Zerrouki, N. Djebli, Meryem Sendjasni, Yasmina Djouder, Fatima Zohra Benali Fellague, Kheira Benouadah","doi":"10.3329/dujps.v19i2.50630","DOIUrl":"https://doi.org/10.3329/dujps.v19i2.50630","url":null,"abstract":"Free radicals, oxidative stress or antioxidants is more and more often used to explain different pathological disorders and their therapeutic approach. The aim of this study was to evaluate the protective effect of anthocyanin extract obtained by maceration of the flowers of Lavandula dentata in 0.1 % HCl/methanol (v/v) solution on oxidative stress. The antioxidant activity of anthocyanin extract in vitro was evaluated by reduction of iron (FRAP), DPPH and the s-carotene tests. The in vivo oxidative stress was induced by intraperitoneal injection of 0.5 ml/kg of CCl4 and treated orally by 500mg kg/day of the extract. Anthocyanins extract inhibited the free radical DPPH (IC50:1.3 ± 0.23 mg/ml). Lavender extract prevented the oxidation of B carotene (28.34 ± 0.07%) and has an ability to reduce iron (0.736 ± 0.03). Intraperitoneal injection of CCl4 has increased biochemical parameters, which was evidence of oxidative stress in vivo. In contrast, daily oral administration of anthocyanin extract has restored the biochemical parameters. Histopathological examinations of liver stained with haematoxylin and eosin showed loss of hepatic architecture. These injuries observed have been improved by treatment with anthocyanin extract. The findings revealed that anthocyanin extract from lavender possesses a significant antioxidant activity. \u0000Dhaka Univ. J. Pharm. Sci. 19(2): 145-151, 2020 (December)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76257105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-10DOI: 10.3329/dujps.v19i2.50631
Emmanuel Eimiomodebheki Odion, R. O. Ogboru, Mike Oshor Ighene
The aim of this study was to identify the chemical constituents of Elaeis guineensis (EG) using GCMS. EG fruits were purchased from a local market in Edo state and was identified, cleaned, boiled and extracted. The resultant crude extract was strained through filter paper and partitioned into n-hexane, dichloromethane and ethyl acetate before subjected to GC-MS analysis. Eighty-two (82) compounds were identified and the major components are hexadecanoic acid, methyl ester (10.53%), oleic acid (25.92%). n-hexadecanoic acid (31.41%), cis-vaccenic acid (22.82%) and octadecanoic acid (13.16%). Plants fatty acids are important for growth and defence mechanism against pathogen, possess antimicrobial action, implicated in pathway elucidation and stimulation of resistance mechanism in plants. Long chain fatty acids exert their effect on membrane phospholipids by modifying its composition and potentially interfering with synthesis of lipid signalling molecules. Identification of EG fruits have provided an idea of its chemical composition of the extract of E. guineensis. Dhaka Univ. J. Pharm. Sci. 19(2): 153-159, 2020 (December)
{"title":"Identification of Compounds in Elaeis guineensis Fruits using GC-MS","authors":"Emmanuel Eimiomodebheki Odion, R. O. Ogboru, Mike Oshor Ighene","doi":"10.3329/dujps.v19i2.50631","DOIUrl":"https://doi.org/10.3329/dujps.v19i2.50631","url":null,"abstract":"The aim of this study was to identify the chemical constituents of Elaeis guineensis (EG) using GCMS. EG fruits were purchased from a local market in Edo state and was identified, cleaned, boiled and extracted. The resultant crude extract was strained through filter paper and partitioned into n-hexane, dichloromethane and ethyl acetate before subjected to GC-MS analysis. Eighty-two (82) compounds were identified and the major components are hexadecanoic acid, methyl ester (10.53%), oleic acid (25.92%). n-hexadecanoic acid (31.41%), cis-vaccenic acid (22.82%) and octadecanoic acid (13.16%). Plants fatty acids are important for growth and defence mechanism against pathogen, possess antimicrobial action, implicated in pathway elucidation and stimulation of resistance mechanism in plants. Long chain fatty acids exert their effect on membrane phospholipids by modifying its composition and potentially interfering with synthesis of lipid signalling molecules. Identification of EG fruits have provided an idea of its chemical composition of the extract of E. guineensis. \u0000Dhaka Univ. J. Pharm. Sci. 19(2): 153-159, 2020 (December)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83744089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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