The main objective of this research was to develop a sustained-release suspension of cinnarizine hydrochloride using raft-forming technique. This innovative approach has been utilized to formulate a series of suspension formulations using hydroxypropyl cellulose (HPC) as a release-retardant polymeric agent. Cinnarizine sustained-release suspensions were prepared by physical mixing method with varying concentrations and combinations of HPC, sodium citrate, sodium saccharin, calcium carbonate, sodium alginate, methyl hydroxybenzoate and propyl hydroxybenzoate. The formulations were subjected for determination of floating time, floating lag time, weight of the raft, physical appearance and in-vitro dissolution. The dissolution was conducted through USP apparatus 2 (paddle type) in 0.1N hydrochloric acid medium having pH 1.2. The key findings of the study demonstrate that a stable sustained-release suspension of cinnarizine can be formulated using raft-forming approach for increased bioavailability and patient-convenience.
{"title":"Study of Release-Retardant Polymers in Formulation Development of Cinnarizine-HCl Sustained-release Oral Suspension Using Raft Technology","authors":"Tabinda Islam, Nusrat Hossain, Mohsina Rahman, Sadia Shabnam, Eyasmin Chowdhury, Syeda Tahsin Nahar, Zarin Tasnim Gias, H. Reza","doi":"10.3329/dujps.v19i1.47814","DOIUrl":"https://doi.org/10.3329/dujps.v19i1.47814","url":null,"abstract":"The main objective of this research was to develop a sustained-release suspension of cinnarizine hydrochloride using raft-forming technique. This innovative approach has been utilized to formulate a series of suspension formulations using hydroxypropyl cellulose (HPC) as a release-retardant polymeric agent. Cinnarizine sustained-release suspensions were prepared by physical mixing method with varying concentrations and combinations of HPC, sodium citrate, sodium saccharin, calcium carbonate, sodium alginate, methyl hydroxybenzoate and propyl hydroxybenzoate. The formulations were subjected for determination of floating time, floating lag time, weight of the raft, physical appearance and in-vitro dissolution. The dissolution was conducted through USP apparatus 2 (paddle type) in 0.1N hydrochloric acid medium having pH 1.2. The key findings of the study demonstrate that a stable sustained-release suspension of cinnarizine can be formulated using raft-forming approach for increased bioavailability and patient-convenience.","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90964055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.3329/dujps.v19i2.50623
S. Sarker, L. Nahar, S. Bachar, M. Rashid
Professor Bidyut Kanti Datta, a renowned professor of the Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, died at the age of 73 on Friday 11 September 2020 in Canada (Canadian time 7 10 am and BD time 5 10 pm), as a consequence of COVID-19 infection followed by pneumonia This article is a brief review of his research work where the authors of this article are immensely proud to be associated with Prof Datta published more than 60 research articles in reputed journals, and the lead author (SDS) of this article, one of his former students from the University of Dhaka, is a co-author of 20 of those publications These 20 publications resulted from a long-standing research collaboration that spanned over two decades, especially research involving various Bangladeshi species of the genus Polygonum L of the family, Polygonaceae, and they demonstrate the breadth and depth of research activities that Prof Datta was involved in, and his long-standing commitment to research that underpinned and enriched his teaching offerings to hundreds of students he taught in higher education sector in Bangladesh
{"title":"Twenty Years, Twenty Publications and could have been More: Revisiting Research Collaboration with Professor Bidyut Kanti Datta","authors":"S. Sarker, L. Nahar, S. Bachar, M. Rashid","doi":"10.3329/dujps.v19i2.50623","DOIUrl":"https://doi.org/10.3329/dujps.v19i2.50623","url":null,"abstract":"Professor Bidyut Kanti Datta, a renowned professor of the Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, died at the age of 73 on Friday 11 September 2020 in Canada (Canadian time 7 10 am and BD time 5 10 pm), as a consequence of COVID-19 infection followed by pneumonia This article is a brief review of his research work where the authors of this article are immensely proud to be associated with Prof Datta published more than 60 research articles in reputed journals, and the lead author (SDS) of this article, one of his former students from the University of Dhaka, is a co-author of 20 of those publications These 20 publications resulted from a long-standing research collaboration that spanned over two decades, especially research involving various Bangladeshi species of the genus Polygonum L of the family, Polygonaceae, and they demonstrate the breadth and depth of research activities that Prof Datta was involved in, and his long-standing commitment to research that underpinned and enriched his teaching offerings to hundreds of students he taught in higher education sector in Bangladesh","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78034186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-12DOI: 10.3329/dujps.v18i2.44467
F. Aktar, Zakir Sultan, M. Rashid
Drug-drug interactions have been a serious concern for pharmacokinetics, pharmacodynamics and pharmacological profiles of therapeutic agents. The aim of this study was to carry out interactions of olmesartan medoxomil with dapagliflozin, vildagliptin and metformin, which were confirmed by TLC, HPLC and FT-IR. The newly formed complexes showed characteristic thermochemical properties in differential scanning calorimetry (DSC) and thermo gravimetric analysis (TGA). In TLC, three spots from the three complexes were found to be different from their precursor drugs. In HPLC chromatograms, the Rt (retention time) of the pure olmesartan medoxomil, dapagliflozin, vildagliptin and metformin were found to be different from their respective complexes. The FT-IR spectra obtained for drug-drug interactions were seen to demonstrate new pattern of peaks compared to pure drugs. The DSC and TGA thermograms of olmesartan medoxomil, dapagliflozin, vildagliptin and metformin were also found to be different from their complexes. All these variations from parent compounds indicated the formation of new complexes.
{"title":"In vitro Complexation of Olmesartan Medoxomil with Dapagliflozin, Vildagliptin and Metformin","authors":"F. Aktar, Zakir Sultan, M. Rashid","doi":"10.3329/dujps.v18i2.44467","DOIUrl":"https://doi.org/10.3329/dujps.v18i2.44467","url":null,"abstract":"Drug-drug interactions have been a serious concern for pharmacokinetics, pharmacodynamics and pharmacological profiles of therapeutic agents. The aim of this study was to carry out interactions of olmesartan medoxomil with dapagliflozin, vildagliptin and metformin, which were confirmed by TLC, HPLC and FT-IR. The newly formed complexes showed characteristic thermochemical properties in differential scanning calorimetry (DSC) and thermo gravimetric analysis (TGA). In TLC, three spots from the three complexes were found to be different from their precursor drugs. In HPLC chromatograms, the Rt (retention time) of the pure olmesartan medoxomil, dapagliflozin, vildagliptin and metformin were found to be different from their respective complexes. The FT-IR spectra obtained for drug-drug interactions were seen to demonstrate new pattern of peaks compared to pure drugs. The DSC and TGA thermograms of olmesartan medoxomil, dapagliflozin, vildagliptin and metformin were also found to be different from their complexes. All these variations from parent compounds indicated the formation of new complexes.","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79794300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-12DOI: 10.3329/dujps.v18i2.44462
A. Shittu, A. Aliyu, David, N. Njinga, Hina Ishaq
In order to authenticate and ascertain the various claim by the rural dwellers that depend on chewing sticks for their oral hygiene and protection against innumerable diseases, the antibacterial activity of the roots of Fagara zanthoxyloides and Distemonanthus benthamianus were evaluated. Preliminary phytochemical screening was carried out using standard methods. In-vitro antibacterial activity of the methanol extracts of both plants was also carried out using the agar well diffusion method against standard strains of Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella typhii ATCC 14028, Citrobacter freundii ATCC 8090 and also some clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis at different concentrations and ratios. Preliminary phytochemical screening showed that the extracts contained alkaloids, saponin, tannins, steroids, flavonoids, anthraquinones, cardiac glycosides and phenol. All the test microorganisms were susceptible to the inhibitory effect of the extracts at concentrations of 200 mg/ml and 500 mg/ml. F. zanthoxyloides had better antimicrobial activity with zones of inhibition ranging from 21.0 to 26.0 mm at 200 mg/ml and 24.3 to 29.3 mm at 500 mg/ml while D. benthamianus had zones of inhibition ranging from 17.7 to 26.7 mm at 200 mg/mL and 19.0 to 26.7 mm at 500 mg/mL. Some of the test microorganisms were resistant to the standard antibiotics (cefuroxime, ciprofloxacin, ofloxacin and gentamicin). Combinations of the extracts in ratios 50:50 and 75:25 yielded no increase in activity. The MIC and MBC for D. benthamianus ranged from 6.25 mg/ml to 100 mg/mL and 25 to 200 mg/ml, respectively while that of F. zanthoxyloides ranged from 1.56 mg/ml to 12.5 mg/mL and 50 to 200 mg/ml, respectively. The antimicrobial activity demonstrated by F. zanthoxyloides and D. benthamianus indicates that they would be valuable in the management of urinary, respiratory and gastrointestinal tracts infections while at the same time helping to mitigate the problem of antimicrobial resistance. Phenolic compounds of plants, among them flavonoids are the chief constituents, which have potent antioxidant activities.
{"title":"Potential Antibacterial Activity of Two Important Local Chewing Sticks “Fagara zanthoxyloides and Distemonanthus benthamianus” along with Antioxidant Capacities","authors":"A. Shittu, A. Aliyu, David, N. Njinga, Hina Ishaq","doi":"10.3329/dujps.v18i2.44462","DOIUrl":"https://doi.org/10.3329/dujps.v18i2.44462","url":null,"abstract":"In order to authenticate and ascertain the various claim by the rural dwellers that depend on chewing sticks for their oral hygiene and protection against innumerable diseases, the antibacterial activity of the roots of Fagara zanthoxyloides and Distemonanthus benthamianus were evaluated. Preliminary phytochemical screening was carried out using standard methods. In-vitro antibacterial activity of the methanol extracts of both plants was also carried out using the agar well diffusion method against standard strains of Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Salmonella typhii ATCC 14028, Citrobacter freundii ATCC 8090 and also some clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis at different concentrations and ratios. Preliminary phytochemical screening showed that the extracts contained alkaloids, saponin, tannins, steroids, flavonoids, anthraquinones, cardiac glycosides and phenol. All the test microorganisms were susceptible to the inhibitory effect of the extracts at concentrations of 200 mg/ml and 500 mg/ml. F. zanthoxyloides had better antimicrobial activity with zones of inhibition ranging from 21.0 to 26.0 mm at 200 mg/ml and 24.3 to 29.3 mm at 500 mg/ml while D. benthamianus had zones of inhibition ranging from 17.7 to 26.7 mm at 200 mg/mL and 19.0 to 26.7 mm at 500 mg/mL. Some of the test microorganisms were resistant to the standard antibiotics (cefuroxime, ciprofloxacin, ofloxacin and gentamicin). Combinations of the extracts in ratios 50:50 and 75:25 yielded no increase in activity. The MIC and MBC for D. benthamianus ranged from 6.25 mg/ml to 100 mg/mL and 25 to 200 mg/ml, respectively while that of F. zanthoxyloides ranged from 1.56 mg/ml to 12.5 mg/mL and 50 to 200 mg/ml, respectively. The antimicrobial activity demonstrated by F. zanthoxyloides and D. benthamianus indicates that they would be valuable in the management of urinary, respiratory and gastrointestinal tracts infections while at the same time helping to mitigate the problem of antimicrobial resistance. Phenolic compounds of plants, among them flavonoids are the chief constituents, which have potent antioxidant activities.","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84137489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-12DOI: 10.3329/dujps.v18i2.44464
L. Roman, Kozachok Solomiia
The aerial part of Astragalus glycyphyllos L. (Astragali glycyphylli herba), a promising nephroprotective agent in folk medicine, has been investigated by HPLC-UV for occurrence of catechins, which are known in individual state as renoprotective substances and gallic acid. The following six compounds were detected and quantitatively determined in the analyzed plant material: (-)-gallocatechin, (-)-epigallocatechin, (+)-catechin, (-)-epicatechin, (-)-epicatechin gallate and gallic acid. The highest yield amongst the flavan-3-ols was found for (-)-gallocatechin (3.7 mg/g) and (-)-epigallocatechin (1.7 mg/g). The determined content of gallic acid was 0.4 mg/g. The investigated material should be subjected for further studies to substantiate the recommendation for its wider employment in current phytotherapeutic practice.
{"title":"HPLC-UV Determination of Catechins and Gallic Acid in Aerial Parts of Astragalus glycyphyllos L.","authors":"L. Roman, Kozachok Solomiia","doi":"10.3329/dujps.v18i2.44464","DOIUrl":"https://doi.org/10.3329/dujps.v18i2.44464","url":null,"abstract":"The aerial part of Astragalus glycyphyllos L. (Astragali glycyphylli herba), a promising nephroprotective agent in folk medicine, has been investigated by HPLC-UV for occurrence of catechins, which are known in individual state as renoprotective substances and gallic acid. The following six compounds were detected and quantitatively determined in the analyzed plant material: (-)-gallocatechin, (-)-epigallocatechin, (+)-catechin, (-)-epicatechin, (-)-epicatechin gallate and gallic acid. The highest yield amongst the flavan-3-ols was found for (-)-gallocatechin (3.7 mg/g) and (-)-epigallocatechin (1.7 mg/g). The determined content of gallic acid was 0.4 mg/g. The investigated material should be subjected for further studies to substantiate the recommendation for its wider employment in current phytotherapeutic practice.","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78539142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-12DOI: 10.3329/dujps.v18i2.44465
Kobirul Islam, Tahrima Arman Tusty, A. Akhand, N. Ahsan
Calcium (Ca) plays important structural and physiological roles in our body. Usually, our body meets up its Ca need from dietary sources. In developing countries like Bangladesh, most of the people cannot afford Carich foods. So, Ca supplements are generally prescribed to deficient individual that usually costs high. It is, therefore, necessary to find out an alternative and cheap source of Ca supplement especially in Bangladesh. Considering the above facts, the main focus of this study was to explore the possibility of using the egg shell as a source of Ca. In his study, discarded eggshells were collected, washed, decontaminated properly and ground to a fine powder. Washing with tap water followed by 10 min boiling completely decontaminated the eggshells. On an average 3.45, 5.91, 4.5 and 4.55 g of eggshell powder (ESP) per shell were obtained from a domesticated fowl, domestic duck, white leghorn, and rhode island red, respectively. Particle size was measured using scanning electron microscope and the size ranged between 3.47-5.94 μm. The Ca contents were found as 38.65, 35.0, 24.85 and 35.73 g in domesticated fowl, domestic duck, white leghorn, and rhode island red, respectively per 100 g of ESP. Taking daily Ca requirements into account, we observed that powder from almost half of an eggshell is sufficient to fulfill the daily Ca need. The amount of magnesium (Mg) in 100 g ESPs was found to be 45.37, 32.48, 45.17 and 45.27 mg and that of iron (Fe) was 35.07, 33.47, 31.91 and 33.06 mg in domesticated fowl, domestic duck, white leghorn, and rhode island red, respectively. Various toxic heavy metals such as chromium (Cr), lead (Pb) and arsenic (As) contents remained below the detection level in ESP samples. Organoleptic tests revealed that the ESP does not have any objectionable effects on the texture and flavor of ESP-fortified foods and juice. These results suggested that the ESP can be used as an alternative and important cheap source of Ca and other beneficial micronutrients for human being. However, it is necessary to create the awareness and public perception regarding the use of EPS.
钙(Ca)在我们的身体中起着重要的结构和生理作用。通常，我们的身体会从饮食中获取所需的钙。在像孟加拉国这样的发展中国家，大多数人买不起富含营养的食物。所以，钙补充剂通常是给缺乏钙的人开的，通常价格很高。因此，有必要找到一种替代和廉价的钙补充剂来源，特别是在孟加拉国。考虑到上述事实，本研究的主要重点是探索将蛋壳作为Ca来源的可能性。在他的研究中，收集废弃的蛋壳，进行洗涤，适当去污并磨成细粉。用自来水冲洗，然后煮沸10分钟，完全去污蛋壳。家禽、家鸭、白来角鸭和罗得岛红的蛋壳粉(ESP)平均每壳含量分别为3.45、5.91、4.5和4.55 g。采用扫描电镜对其粒度进行测定，粒径范围为3.47 ~ 5.94 μm。在家禽、家鸭、白来角鸭和罗得岛红中，每100 g ESP的钙含量分别为38.65、35.0、24.85和35.73 g。考虑到日钙需求，我们发现几乎一半蛋壳中的粉末就足以满足日钙需求。100 g ESPs中，家禽、家鸭、白来角鸭和罗得岛红的镁含量分别为45.37、32.48、45.17和45.27 Mg，铁含量分别为35.07、33.47、31.91和33.06 Mg。ESP样品中铬(Cr)、铅(Pb)、砷(as)等多种有毒重金属含量均低于检测水平。感官测试显示，ESP对ESP强化食品和果汁的质地和风味没有任何不良影响。这些结果表明，ESP可以作为人类钙和其他有益微量元素的替代和廉价来源。然而，有必要建立公众对使用易办事的认识和看法。
{"title":"Human Uptake of Eggshell Powder as an Alternate Source of Calcium","authors":"Kobirul Islam, Tahrima Arman Tusty, A. Akhand, N. Ahsan","doi":"10.3329/dujps.v18i2.44465","DOIUrl":"https://doi.org/10.3329/dujps.v18i2.44465","url":null,"abstract":"Calcium (Ca) plays important structural and physiological roles in our body. Usually, our body meets up its Ca need from dietary sources. In developing countries like Bangladesh, most of the people cannot afford Carich foods. So, Ca supplements are generally prescribed to deficient individual that usually costs high. It is, therefore, necessary to find out an alternative and cheap source of Ca supplement especially in Bangladesh. Considering the above facts, the main focus of this study was to explore the possibility of using the egg shell as a source of Ca. In his study, discarded eggshells were collected, washed, decontaminated properly and ground to a fine powder. Washing with tap water followed by 10 min boiling completely decontaminated the eggshells. On an average 3.45, 5.91, 4.5 and 4.55 g of eggshell powder (ESP) per shell were obtained from a domesticated fowl, domestic duck, white leghorn, and rhode island red, respectively. Particle size was measured using scanning electron microscope and the size ranged between 3.47-5.94 μm. The Ca contents were found as 38.65, 35.0, 24.85 and 35.73 g in domesticated fowl, domestic duck, white leghorn, and rhode island red, respectively per 100 g of ESP. Taking daily Ca requirements into account, we observed that powder from almost half of an eggshell is sufficient to fulfill the daily Ca need. The amount of magnesium (Mg) in 100 g ESPs was found to be 45.37, 32.48, 45.17 and 45.27 mg and that of iron (Fe) was 35.07, 33.47, 31.91 and 33.06 mg in domesticated fowl, domestic duck, white leghorn, and rhode island red, respectively. Various toxic heavy metals such as chromium (Cr), lead (Pb) and arsenic (As) contents remained below the detection level in ESP samples. Organoleptic tests revealed that the ESP does not have any objectionable effects on the texture and flavor of ESP-fortified foods and juice. These results suggested that the ESP can be used as an alternative and important cheap source of Ca and other beneficial micronutrients for human being. However, it is necessary to create the awareness and public perception regarding the use of EPS.","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82927125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-12DOI: 10.3329/dujps.v18i2.44463
W. Obonga, C. Nnadi, Chinonye C. Chima, S. Okafor, E. O. Omeje
This study evaluated the antioxidant and anti-inflammatory properties of Marantochloa leucantha (Marantaceae). The in vitro antioxidant activity of the extracts and solvent fractions was evaluated by 1,1-diphenyl2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant potential (FRAP) assay models and in vivo anti-inflammatory activity by the rat paw edema model. The phytochemical screening indicated the presence of tannins, terpenoids, steroids, flavonoids, reducing sugar and phenolics. The antioxidant assay showed that all the extracts exhibited high antioxidant activity comparable with ascorbic and gallic acid controls. In DPPH model, a 250 μg/ml EtOAc fraction of the leaves showed antioxidant activity of 93.9 ± 1.7 % (EC50 0.82 μg/ml) and a 1000 μg/ml of same stem fraction produced 91.9 ± 0.3 % activity (EC50 1.38 μg/ml). In the FRAP model, EtOAc fraction exhibited 31.1±0.7 and 92.0 ± 2.2 μM Fe/g of dried leaves and stem, respectively at 1000 μg/ml FeSO4 equivalent. The anti-inflammatory potential of the plant showed that the crude stem extract and fractions at 200 600 mg/kg exhibited significant (p < 0.01) dose-related inhibition of paw edema in rats. A 200 mg/kg EtOAc fraction showed 18.8 % inhibition compared to 31 % observed in diclofenac-treated rats in 2 h post albumin challenge. These findings validated the folkloric use of this plant in the treatment of diseases associated to the oxidative stress and could further provide promising lead compounds with potent antioxidant and anti-inflammatory activities.
{"title":"In- vitro antioxidant and In vivo Anti-inflammatory Potentials of Marantochloa Leucantha (Marantaceae) Extracts and Fractions","authors":"W. Obonga, C. Nnadi, Chinonye C. Chima, S. Okafor, E. O. Omeje","doi":"10.3329/dujps.v18i2.44463","DOIUrl":"https://doi.org/10.3329/dujps.v18i2.44463","url":null,"abstract":"This study evaluated the antioxidant and anti-inflammatory properties of Marantochloa leucantha (Marantaceae). The in vitro antioxidant activity of the extracts and solvent fractions was evaluated by 1,1-diphenyl2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant potential (FRAP) assay models and in vivo anti-inflammatory activity by the rat paw edema model. The phytochemical screening indicated the presence of tannins, terpenoids, steroids, flavonoids, reducing sugar and phenolics. The antioxidant assay showed that all the extracts exhibited high antioxidant activity comparable with ascorbic and gallic acid controls. In DPPH model, a 250 μg/ml EtOAc fraction of the leaves showed antioxidant activity of 93.9 ± 1.7 % (EC50 0.82 μg/ml) and a 1000 μg/ml of same stem fraction produced 91.9 ± 0.3 % activity (EC50 1.38 μg/ml). In the FRAP model, EtOAc fraction exhibited 31.1±0.7 and 92.0 ± 2.2 μM Fe/g of dried leaves and stem, respectively at 1000 μg/ml FeSO4 equivalent. The anti-inflammatory potential of the plant showed that the crude stem extract and fractions at 200 600 mg/kg exhibited significant (p < 0.01) dose-related inhibition of paw edema in rats. A 200 mg/kg EtOAc fraction showed 18.8 % inhibition compared to 31 % observed in diclofenac-treated rats in 2 h post albumin challenge. These findings validated the folkloric use of this plant in the treatment of diseases associated to the oxidative stress and could further provide promising lead compounds with potent antioxidant and anti-inflammatory activities.","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89388489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-12DOI: 10.3329/dujps.v18i2.44466
Mahbubul Alam, Faria Tasneem, A. Kabir, A. S. Rouf
A well-established drug used in the management of inflammatory bowel disease is 5-aminosalicylic acid (e.g. Mesalazine or Mesalamine). For the treatment of mild to moderate flares of ulcerative colitis and Crohn’s disease, Mesalazine has been used as the first line drug in both western and Asian countries due to its superiority over other drugs in terms of side effects and toxicities. Besides, some other drugs are also prescribed for total resolution of different symptoms of ulcerative colitis and associated diseases, which include Acetaminophen, Metronidazole and Vitamin D3. Moreover, physicians instruct that Mesalazine should be taken at least one hour before meal. So, there are enough scopes of studying the drug-food interaction of Mesalazine to assess if there is any incompatibility present with food. Thus, in the present study, Mesalazine and physical mixtures of Mesalazine (1:1) in solid form along with the aforementioned drugs were prepared and analyzed to evaluate the compatibility among them using Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC). In addition, interactions of Mesalazine with food stocks such as chicken and vegetable broth, fruit juice, milk and soybean oil were studied using FTIR, considering these as the common sources of protein, vitamin, fiber, minerals and fat. From this study, it was interpreted that, major interactions of Mesalazine were present with food samples. Besides, FTIR and DSC data revealed subtle clues of incompatibilities between Mesalazine and the other two drugs except Vitamin D3. So, the results may prove to be useful for related research works in future.
{"title":"Study of Drug-Drug and Drug-Food Interactions of Mesalazine Through FTIR and DSC","authors":"Mahbubul Alam, Faria Tasneem, A. Kabir, A. S. Rouf","doi":"10.3329/dujps.v18i2.44466","DOIUrl":"https://doi.org/10.3329/dujps.v18i2.44466","url":null,"abstract":"A well-established drug used in the management of inflammatory bowel disease is 5-aminosalicylic acid (e.g. Mesalazine or Mesalamine). For the treatment of mild to moderate flares of ulcerative colitis and Crohn’s disease, Mesalazine has been used as the first line drug in both western and Asian countries due to its superiority over other drugs in terms of side effects and toxicities. Besides, some other drugs are also prescribed for total resolution of different symptoms of ulcerative colitis and associated diseases, which include Acetaminophen, Metronidazole and Vitamin D3. Moreover, physicians instruct that Mesalazine should be taken at least one hour before meal. So, there are enough scopes of studying the drug-food interaction of Mesalazine to assess if there is any incompatibility present with food. Thus, in the present study, Mesalazine and physical mixtures of Mesalazine (1:1) in solid form along with the aforementioned drugs were prepared and analyzed to evaluate the compatibility among them using Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC). In addition, interactions of Mesalazine with food stocks such as chicken and vegetable broth, fruit juice, milk and soybean oil were studied using FTIR, considering these as the common sources of protein, vitamin, fiber, minerals and fat. From this study, it was interpreted that, major interactions of Mesalazine were present with food samples. Besides, FTIR and DSC data revealed subtle clues of incompatibilities between Mesalazine and the other two drugs except Vitamin D3. So, the results may prove to be useful for related research works in future.","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85049909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-22DOI: 10.3329/dujps.v18i2.43260
Q. S. Islam, H. Sohrab, S. Sharmin, C. Hasan
Repeated chromatographic separation and purification of the dichloromethane-methanol (1:1) extract of the stem bark of Calophyllum inophyllum led to the isolation of four secondary metabolites which were identified as β-friedelin (1), α-friedelan-3-ol (2), 4-hydroxyxanthone (3) and stigmast-5-en-3-one (4) by analysis of their NMR data and comparison with published values Dhaka Univ. J. Pharm. Sci. 18(2): 179-182, 2019 (December)
{"title":"Phytochemical Investigation of Calophyllum inophyllum L.","authors":"Q. S. Islam, H. Sohrab, S. Sharmin, C. Hasan","doi":"10.3329/dujps.v18i2.43260","DOIUrl":"https://doi.org/10.3329/dujps.v18i2.43260","url":null,"abstract":"Repeated chromatographic separation and purification of the dichloromethane-methanol (1:1) extract of the stem bark of Calophyllum inophyllum led to the isolation of four secondary metabolites which were identified as β-friedelin (1), α-friedelan-3-ol (2), 4-hydroxyxanthone (3) and stigmast-5-en-3-one (4) by analysis of their NMR data and comparison with published values \u0000Dhaka Univ. J. Pharm. Sci. 18(2): 179-182, 2019 (December)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77703850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-22DOI: 10.3329/dujps.v18i2.43257
J. Hasan, R. Rabbani, S. C. Bachar
Teicoplanin is a drug of choice for treating infections by gram positive microorganisms. Teicoplanininduced thrombocytopenia is an adverse drug reaction found in critically ill patients receiving teicoplanin. The aim of this observational study was to analyze the increased risk of occurring teicoplanin-induced thrombocytopenia in patients with severe hypoalbuminemia than the patients having normal blood-albumin level. A 5-month long study was conducted in an adult-ICU department on 42 critically ill adult patients. In this study, 17 (40.48%, n=42) patients developed teicoplanin-induced thrombocytopenia and among them, 14 patients (60.87%, n=23) were suffering from severe hypoalbuminemia during the initiation of teicoplanin therapy. All the events were normalized within 48 hours after discontinuing the teicoplanin. In this study, we found that teicoplanin-induced thrombocytopenia is one of the most common adverse drug reaction developed in critically ill adult patients and concurrent severe hypoalbuminemia may enormously increase the risk of this adverse drug reaction. Dhaka Univ. J. Pharm. Sci. 18(2): 153-157, 2019 (December)
{"title":"Severe Hypoalbuminemia Increases the Risk of Thrombocytopenia in Critically Ill Adult Patients Receiving Teicoplanin","authors":"J. Hasan, R. Rabbani, S. C. Bachar","doi":"10.3329/dujps.v18i2.43257","DOIUrl":"https://doi.org/10.3329/dujps.v18i2.43257","url":null,"abstract":"Teicoplanin is a drug of choice for treating infections by gram positive microorganisms. Teicoplanininduced thrombocytopenia is an adverse drug reaction found in critically ill patients receiving teicoplanin. The aim of this observational study was to analyze the increased risk of occurring teicoplanin-induced thrombocytopenia in patients with severe hypoalbuminemia than the patients having normal blood-albumin level. A 5-month long study was conducted in an adult-ICU department on 42 critically ill adult patients. In this study, 17 (40.48%, n=42) patients developed teicoplanin-induced thrombocytopenia and among them, 14 patients (60.87%, n=23) were suffering from severe hypoalbuminemia during the initiation of teicoplanin therapy. All the events were normalized within 48 hours after discontinuing the teicoplanin. In this study, we found that teicoplanin-induced thrombocytopenia is one of the most common adverse drug reaction developed in critically ill adult patients and concurrent severe hypoalbuminemia may enormously increase the risk of this adverse drug reaction. \u0000Dhaka Univ. J. Pharm. Sci. 18(2): 153-157, 2019 (December)","PeriodicalId":11304,"journal":{"name":"Dhaka University Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84568858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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