Lumbosacral hydatid disease (LHD), a rare skeletal parasitic disease that involves the lumbosacral region. In this study, we summarized the diagnostic and therapeutic procedures for patients with LHD to provide insights into managing this rare disease.
Methods
Between 2000 and 2023, 16 patients diagnosed with LHD were retrospectively analyzed. Each patient's medical records and follow-up details, were carefully assessed. The average follow-up period was 11.25 ± 6.41 years, providing valuable insights into treatment durability and effectiveness.
Results
The diagnosis was confirmed via imaging, serological tests, and pathological examination. The clinical symptoms included lumbago with lower limb numbness (25 %) and urinary and fecal incontinence (25 %). All patients underwent surgery, with an average of 2.6 surgeries per patient. Thirteen (81.25 %) patients experienced recurrence postoperatively.
Conclusion
LHD is a severe and complex skeletal parasitic disease with significant diagnostic and therapeutic challenges. Effective management requires a comprehensive strategy involving surgery and additional therapies.
{"title":"Challenges and pitfalls in managing lumbosacral hydatid disease: Lessons learned from clinical practice","authors":"Wubulikasimu Mijiti , Xin Wang , Qiyu Jia , Yansong Li, Ziyu Zhou, Tayier Reheman, Yelinaer Ayiheng, Shimin Dong, Zengru Xie","doi":"10.1016/j.diagmicrobio.2024.116542","DOIUrl":"10.1016/j.diagmicrobio.2024.116542","url":null,"abstract":"<div><h3>Objective</h3><div>Lumbosacral hydatid disease (LHD), a rare skeletal parasitic disease that involves the lumbosacral region. In this study, we summarized the diagnostic and therapeutic procedures for patients with LHD to provide insights into managing this rare disease.</div></div><div><h3>Methods</h3><div>Between 2000 and 2023, 16 patients diagnosed with LHD were retrospectively analyzed. Each patient's medical records and follow-up details, were carefully assessed. The average follow-up period was 11.25 ± 6.41 years, providing valuable insights into treatment durability and effectiveness.</div></div><div><h3>Results</h3><div>The diagnosis was confirmed via imaging, serological tests, and pathological examination. The clinical symptoms included lumbago with lower limb numbness (25 %) and urinary and fecal incontinence (25 %). All patients underwent surgery, with an average of 2.6 surgeries per patient. Thirteen (81.25 %) patients experienced recurrence postoperatively.</div></div><div><h3>Conclusion</h3><div>LHD is a severe and complex skeletal parasitic disease with significant diagnostic and therapeutic challenges. Effective management requires a comprehensive strategy involving surgery and additional therapies.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116542"},"PeriodicalIF":2.1,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.diagmicrobio.2024.116543
Simon Lévesque , Samuel Rouleau , Danny Bergeron , Nathalie Brown , Sadjia Bekal , Cindy Lalancette , Isabelle Alarie
We report the characterization of Xenophilus aerolatus strain identified from a screening rectal swab specimen in human, initially misidentified as Brucella spp. by MALDI-TOF MS (VITEK-MS system). The strain is able to growth on ColorexTM mSuperCARBA plate, suggesting carbapenem resistance. Whole genome sequencing identified several potential antimicrobial resistance mechanisms.
{"title":"Xenophilus aerolatus isolate misidentified as Brucella spp. by MALDI-TOF MS (VITEK-MS) system","authors":"Simon Lévesque , Samuel Rouleau , Danny Bergeron , Nathalie Brown , Sadjia Bekal , Cindy Lalancette , Isabelle Alarie","doi":"10.1016/j.diagmicrobio.2024.116543","DOIUrl":"10.1016/j.diagmicrobio.2024.116543","url":null,"abstract":"<div><div>We report the characterization of <em>Xenophilus aerolatus</em> strain identified from a screening rectal swab specimen in human, initially misidentified as <em>Brucella</em> spp. by MALDI-TOF MS (VITEK-MS system). The strain is able to growth on Colorex<sup>TM</sup> mSuperCARBA plate, suggesting carbapenem resistance. Whole genome sequencing identified several potential antimicrobial resistance mechanisms.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116543"},"PeriodicalIF":2.1,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0732889324003687/pdfft?md5=30a4aeb69f7ecde052d5b21b84ece913&pid=1-s2.0-S0732889324003687-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-19DOI: 10.1016/j.diagmicrobio.2024.116540
Wei Wang , Hongya Gong , Xiaoxuan Yang , Lingjuan Xu , Xiaoyu Huang , Guanyu Su , Yongyao Tan , Bihui Jing , Zhifen Zheng , Zhongju Chen , Ping Wang , Guigang Li
Colletotrichum is a relatively uncommon cause of human infection. Previous findings on Colletotrichum keratitis were scarce, and most diagnoses were based on morphological distinction, perhaps underestimating the incidence of Colletotrichum species. This research describes the clinical characteristics and treatment outcomes of 9 cases of Colletotrichum keratitis discovered in our hospital using next-generation sequencing (NGS).We reviewed 78 patients with NGS-proven fungal keratitis between September 1, 2021 and May 31, 2023, 9 patients (11.5 %) were verified as infected with Colletotrichum species, and their medical records were reviewed to identify the clinical characteristics. NGS revealed that 3 patients were infected with C. truncatum, 3 patients with C. gloeosporioides, and the other 3 patients with C. fructicola. Seven patients had a history of corneal plant trauma (all three patients with C. fructicola had corneal injury history due to chestnut burrs), one patient was infected by mosquitoes flying into the eye, and one patient had an unknown origin. Seven patients underwent penetrating keratoplasty, while two patients underwent lamellar keratoplasty. Eight patients healed after keratoplasty, but one required evisceration due to recurrence of fungal infection in the anterior chamber and intractable discomfort.In conclusion NGS allows for more precise diagnosis and enhances epidemiological awareness of Colletotrichum keratitis, which is not as rare as previously reported.
{"title":"Colletotrichum keratitis: An important fungal infection of nine human eyes","authors":"Wei Wang , Hongya Gong , Xiaoxuan Yang , Lingjuan Xu , Xiaoyu Huang , Guanyu Su , Yongyao Tan , Bihui Jing , Zhifen Zheng , Zhongju Chen , Ping Wang , Guigang Li","doi":"10.1016/j.diagmicrobio.2024.116540","DOIUrl":"10.1016/j.diagmicrobio.2024.116540","url":null,"abstract":"<div><div><em>Colletotrichum</em> is a relatively uncommon cause of human infection. Previous findings on <em>Colletotrichum</em> keratitis were scarce, and most diagnoses were based on morphological distinction, perhaps underestimating the incidence of <em>Colletotrichum</em> species. This research describes the clinical characteristics and treatment outcomes of 9 cases of <em>Colletotrichum</em> keratitis discovered in our hospital using next-generation sequencing (NGS).We reviewed 78 patients with NGS-proven fungal keratitis between September 1, 2021 and May 31, 2023, 9 patients (11.5 %) were verified as infected with <em>Colletotrichum</em> species, and their medical records were reviewed to identify the clinical characteristics. NGS revealed that 3 patients were infected with <em>C. truncatum</em>, 3 patients with <em>C. gloeosporioides</em>, and the other 3 patients with <em>C. fructicola</em>. Seven patients had a history of corneal plant trauma (all three patients with <em>C. fructicola</em> had corneal injury history due to chestnut burrs), one patient was infected by mosquitoes flying into the eye, and one patient had an unknown origin. Seven patients underwent penetrating keratoplasty, while two patients underwent lamellar keratoplasty. Eight patients healed after keratoplasty, but one required evisceration due to recurrence of fungal infection in the anterior chamber and intractable discomfort.In conclusion NGS allows for more precise diagnosis and enhances epidemiological awareness of <em>Colletotrichum</em> keratitis, which is not as rare as previously reported.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116540"},"PeriodicalIF":2.1,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-17DOI: 10.1016/j.diagmicrobio.2024.116541
Negar Jamshidi , Melissa Waine , Monique Binet , Vathsala Mohan , David J Carter , Branwen Morgan
Despite technological advances and readily available point of care test (POCT) devices with rapid turn-around results for respiratory tract infection (RTI) management, their adoption in primary care remains low. This paper summarises the challenges and facilitators of POCT implementation for RTIs in primary care settings in high-income countries. The review of 28 studies identified by systematic searches of electronic databases improves our understanding of the current state and will help guide the design and implementation of strategies to improve widespread POCT adoption. To effectively implement respiratory POCT in primary care, it is crucial to address several key challenges. These include ensuring the availability of resources to alleviate time pressures and costs, enhancing training, increasing quality control, improving device feasibility, and managing patient expectations. In doing so, diagnostic POCTs can contribute to an accurate, rapid, and evidence-based diagnosis of RTIs to reduce antimicrobial use and improve antimicrobial stewardship and patient outcomes.
{"title":"The adoption of point of care testing technologies for respiratory tract infections in primary care in Australia: Challenges and facilitators","authors":"Negar Jamshidi , Melissa Waine , Monique Binet , Vathsala Mohan , David J Carter , Branwen Morgan","doi":"10.1016/j.diagmicrobio.2024.116541","DOIUrl":"10.1016/j.diagmicrobio.2024.116541","url":null,"abstract":"<div><div>Despite technological advances and readily available point of care test (POCT) devices with rapid turn-around results for respiratory tract infection (RTI) management, their adoption in primary care remains low. This paper summarises the challenges and facilitators of POCT implementation for RTIs in primary care settings in high-income countries. The review of 28 studies identified by systematic searches of electronic databases improves our understanding of the current state and will help guide the design and implementation of strategies to improve widespread POCT adoption. To effectively implement respiratory POCT in primary care, it is crucial to address several key challenges. These include ensuring the availability of resources to alleviate time pressures and costs, enhancing training, increasing quality control, improving device feasibility, and managing patient expectations. In doing so, diagnostic POCTs can contribute to an accurate, rapid, and evidence-based diagnosis of RTIs to reduce antimicrobial use and improve antimicrobial stewardship and patient outcomes.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116541"},"PeriodicalIF":2.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-14DOI: 10.1016/j.diagmicrobio.2024.116538
Robert F. Potter , Jamie Marino , Carol E. Muenks , Matthew Lammers , Meghan A. Wallace , Jennifer Dien Bard , Tanis C. Dingle , Romney Humphries , Lars F. Westblade , Carey-Ann D. Burnham
Staphylococcus saprophyticus, a common uropathogen, is usually susceptible to urine-concentrating antimicrobials, so routine AST is not recommended by CLSI. Our study evaluated the antimicrobial resistance profiles of 277 S. saprophyticus isolates from North America and a globally diverse cohort. Notably, 24% (67/277) of our isolates come from non-urinary sources. AST was performed against 12 antimicrobials using standard disk diffusion, PCR for mecA and mecC, PBP2a production assays, and cefinase. 5% (13/277) of isolates were mecA positive and cefinase positive, 63% (176/277) were mecA negative but cefinase positive, 4% (11/277) were mecA positive but cefinase negative, and 28% (77/277) were mecA and cefinase negative. All (277/277) isolates were susceptible to delafloxacin, ciprofloxacin, rifampin, linezolid, and nitrofurantoin and 95% (262/277) were susceptible to trimethoprim-sulfamethoxazole. Our results showed that regardless of using CLSI or EUCAST breakpoints oxacillin had low categorical agreement for mecA presence, making it unsuitable for surrogate testing, while cefoxitin disk diffusion had high very major error rate. If possible, PBP2a or mecA testing is recommended for guiding therapy for non-urinary infections. Our work supports CLSI guidelines on routine susceptibility to urinary tract antibiotics.
{"title":"Detection of mecA-mediated methicillin resistance and evaluation of disk-diffusion antimicrobial susceptibility characteristics of Staphylococcus saprophyticus isolates from geographically diverse locations","authors":"Robert F. Potter , Jamie Marino , Carol E. Muenks , Matthew Lammers , Meghan A. Wallace , Jennifer Dien Bard , Tanis C. Dingle , Romney Humphries , Lars F. Westblade , Carey-Ann D. Burnham","doi":"10.1016/j.diagmicrobio.2024.116538","DOIUrl":"10.1016/j.diagmicrobio.2024.116538","url":null,"abstract":"<div><p><em>Staphylococcus saprophyticus</em>, a common uropathogen, is usually susceptible to urine-concentrating antimicrobials, so routine AST is not recommended by CLSI. Our study evaluated the antimicrobial resistance profiles of 277 <em>S. saprophyticus</em> isolates from North America and a globally diverse cohort. Notably, 24% (67/277) of our isolates come from non-urinary sources. AST was performed against 12 antimicrobials using standard disk diffusion, PCR for <em>mecA</em> and <em>mecC</em>, PBP2a production assays, and cefinase. 5% (13/277) of isolates were <em>mecA</em> positive and cefinase positive, 63% (176/277) were <em>mecA</em> negative but cefinase positive, 4% (11/277) were mecA positive but cefinase negative, and 28% (77/277) were <em>mecA</em> and cefinase negative. All (277/277) isolates were susceptible to delafloxacin, ciprofloxacin, rifampin, linezolid, and nitrofurantoin and 95% (262/277) were susceptible to trimethoprim-sulfamethoxazole. Our results showed that regardless of using CLSI or EUCAST breakpoints oxacillin had low categorical agreement for <em>mecA</em> presence, making it unsuitable for surrogate testing, while cefoxitin disk diffusion had high very major error rate. If possible, PBP2a or <em>mecA</em> testing is recommended for guiding therapy for non-urinary infections. Our work supports CLSI guidelines on routine susceptibility to urinary tract antibiotics.</p></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116538"},"PeriodicalIF":2.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-13DOI: 10.1016/j.diagmicrobio.2024.116539
Juan Añón-Hidalgo , María Garrido-Rodríguez , María José González-Abad , Margarita Cuervas-Mons Vendrell , Marta Taida García-Ascaso , David Díaz Pérez , Alberto García-Salido
Severe infections caused by multidrug-resistant bacteria have underscored the urgent need for innovative treatment approaches. Novel antimicrobials like cefiderocol have emerged as effective options, but their use in children remains largely unexplored. In this brief report, we describe a severe case of sepsis in a child with an oncohematological disease, caused by a highly drug-resistant strain of Klebsiella pneumoniae. The addition of cefiderocol to other therapies resulted in a successful outcome. Additionally, we provide a literature review of previously published cases involving children treated with this new antibiotic. In our patient, cefiderocol was both safe and effective in combating the multidrug-resistant pathogen. However, further research is needed to better define the indications and safety profile of this novel antibiotic.
{"title":"Cefiderocol as rescue therapy in a cancer immunosuppressed critically ill child: Case description and literature review","authors":"Juan Añón-Hidalgo , María Garrido-Rodríguez , María José González-Abad , Margarita Cuervas-Mons Vendrell , Marta Taida García-Ascaso , David Díaz Pérez , Alberto García-Salido","doi":"10.1016/j.diagmicrobio.2024.116539","DOIUrl":"10.1016/j.diagmicrobio.2024.116539","url":null,"abstract":"<div><p>Severe infections caused by multidrug-resistant bacteria have underscored the urgent need for innovative treatment approaches. Novel antimicrobials like cefiderocol have emerged as effective options, but their use in children remains largely unexplored. In this brief report, we describe a severe case of sepsis in a child with an oncohematological disease, caused by a highly drug-resistant strain of <em>Klebsiella pneumoniae</em>. The addition of cefiderocol to other therapies resulted in a successful outcome. Additionally, we provide a literature review of previously published cases involving children treated with this new antibiotic. In our patient, cefiderocol was both safe and effective in combating the multidrug-resistant pathogen<del>.</del> However, further research is needed to better define the indications and safety profile of this novel antibiotic.</p></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116539"},"PeriodicalIF":2.1,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-13DOI: 10.1016/j.diagmicrobio.2024.116530
Abdullah M. Assiri , Amal M Alshahrani , Hussam Sakkijha , Abdulrahman AlGeer , Mohammed Zeitouni , Mohamed AlGohary , Liliane Dhaini , Rashi Verma , Harmandeep Singh
With the evident increased prevalence of respiratory tract infections (RTIs) such as Respiratory Syncytial Virus (RSV), influenza, Group A Streptococcus (GAS), and COVID-19, the conventional diagnostic methods are considered sub-optimal in providing timely management to patients in the Kingdom of Saudi Arabia (KSA). Gaps in current diagnostics are magnified by the Kingdom's unique demographic composition, comprising 11.9 million foreign workers, and the annual influx of over 10 million pilgrims. Current gaps in timely diagnosis leads to delays in treatment, misuse of antibiotics, and protracted hospital stays, subsequently compromising patient care, and escalating healthcare costs. KSA healthcare stakeholders suggest that the integration of rapid molecular Point-of-Care Testing (POCT) into the Kingdom's healthcare infrastructure is an absolute necessity. This publication serves as an urgent call for action aimed at healthcare policymakers in Saudi Arabia, to review the existing diagnostic challenges and include rapid POCTs in the Saudi healthcare strategy for respiratory infections.
{"title":"Transforming respiratory tract infection diagnosis in the kingdom of saudi arabia through point-of-care testing: A white paper for policy makers","authors":"Abdullah M. Assiri , Amal M Alshahrani , Hussam Sakkijha , Abdulrahman AlGeer , Mohammed Zeitouni , Mohamed AlGohary , Liliane Dhaini , Rashi Verma , Harmandeep Singh","doi":"10.1016/j.diagmicrobio.2024.116530","DOIUrl":"10.1016/j.diagmicrobio.2024.116530","url":null,"abstract":"<div><div>With the evident increased prevalence of respiratory tract infections (RTIs) such as Respiratory Syncytial Virus (RSV), influenza, Group A <em>Streptococcus</em> (GAS), and COVID-19, the conventional diagnostic methods are considered sub-optimal in providing timely management to patients in the Kingdom of Saudi Arabia (KSA). Gaps in current diagnostics are magnified by the Kingdom's unique demographic composition, comprising 11.9 million foreign workers, and the annual influx of over 10 million pilgrims. Current gaps in timely diagnosis leads to delays in treatment, misuse of antibiotics, and protracted hospital stays, subsequently compromising patient care, and escalating healthcare costs. KSA healthcare stakeholders suggest that the integration of rapid molecular Point-of-Care Testing (POCT) into the Kingdom's healthcare infrastructure is an absolute necessity. This publication serves as an urgent call for action aimed at healthcare policymakers in Saudi Arabia, to review the existing diagnostic challenges and include rapid POCTs in the Saudi healthcare strategy for respiratory infections.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116530"},"PeriodicalIF":2.1,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-12DOI: 10.1016/j.diagmicrobio.2024.116537
Isabel Guida Smidt-Nielsen , Jonas Bredtoft Boel , Heidi Meiniche , Andreas Petersen , Christian Salgård , Mette Damkjær Bartels , Barbara Juliane Holzknecht
We examined the added value of perineal swabs in addition to nose and throat swabs when screening for Methicillin-resistant Staphylococcus aureus colonization, and risk factors for perineal carriage in 6,642 patients. In our mainly primary care setting, the added value was 9.3%. Patients <3 or ≥80 had the highest risk.
{"title":"The added value of perineal swabs when screening for asymptomatic methicillin-resistant Staphylococcus aureus colonization and risk factors for perineal carriage","authors":"Isabel Guida Smidt-Nielsen , Jonas Bredtoft Boel , Heidi Meiniche , Andreas Petersen , Christian Salgård , Mette Damkjær Bartels , Barbara Juliane Holzknecht","doi":"10.1016/j.diagmicrobio.2024.116537","DOIUrl":"10.1016/j.diagmicrobio.2024.116537","url":null,"abstract":"<div><p>We examined the added value of perineal swabs in addition to nose and throat swabs when screening for Methicillin-resistant <em>Staphylococcus aureus</em> colonization, and risk factors for perineal carriage in 6,642 patients. In our mainly primary care setting, the added value was 9.3%. Patients <3 or ≥80 had the highest risk.</p></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116537"},"PeriodicalIF":2.1,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0732889324003626/pdfft?md5=fe9286cc669f5ff95a4c24ad87b8b07b&pid=1-s2.0-S0732889324003626-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparing serum and plasma samples in the performance of Bordier Affinity IgG ELISA-based assays for diagnosis of Schistosoma- and Strongyloides infection","authors":"Ioana Bujila , Isabelle Eliasson , Emmeli Bång , Anna Färnert , Caroline Rönnberg","doi":"10.1016/j.diagmicrobio.2024.116524","DOIUrl":"10.1016/j.diagmicrobio.2024.116524","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116524"},"PeriodicalIF":2.1,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S073288932400350X/pdfft?md5=2a5cbd30428d8887490c3cfb7880e8b3&pid=1-s2.0-S073288932400350X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142172173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-11DOI: 10.1016/j.diagmicrobio.2024.116526
L. Elaine Epperson , Rebecca M. Davidson , Sara M. Kammlade , Nabeeh A. Hasan , Sophie E. Nick , Iara M.P. Machado , Valerie H. Rodriguez , Aaron Appleman , Niels K. Helstrom , Michael Strong
Pulmonary nontuberculous mycobacteria (NTM) disease is an emerging public health challenge that is especially problematic in people with cystic fibrosis (CF). Effective treatment depends on accurate species and subspecies identification and antimicrobial susceptibility status. We evaluated the GenoType NTM-DR VER 1.0 assay using biobanked NTM isolates with whole genome sequence (WGS) data and control isolates (total n=285). Species and subspecies detection sensitivity and specificity were 100 % for all species and subspecies except for two subspecies of M. intracellulare, that demonstrated a small degree of discrepant identification between M. intracellulare subspecies intracellulare and subspecies chimaera. All antimicrobial resistance markers were identified with 100 % sensitivity and specificity.
We conclude that the GenoType NTM-DR assay offers a rapid and accurate option for identifying the most frequently encountered pathogenic NTM taxa and drug resistance markers.
SUPPORT: Colorado CF Research Development Program and Colorado CF National Resource Centers funded by the Cystic Fibrosis Foundation, NJH Advanced Diagnostics Laboratories, Colorado Advanced Industries Accelerator Grant
肺非结核分枝杆菌（NTM）疾病是一项新出现的公共卫生挑战，尤其是囊性纤维化（CF）患者。有效的治疗取决于准确的菌种和亚种鉴定以及抗菌药敏感性状况。我们使用具有全基因组序列（WGS）数据的生物库 NTM 分离物和对照分离物（总数 n=285）对 GenoType NTM-DR VER 1.0 检测法进行了评估。对所有菌种和亚种的检测灵敏度和特异性均为 100%，但对 M. intracellulare 的两个亚种除外，因为这两个亚种在 M. intracellulare 亚种 intracellulare 和亚种 chimaera 之间的鉴定存在小程度的差异。我们的结论是，GenoType NTM-DR 检测法为鉴定最常见的致病性 NTM 分类群和耐药性标记物提供了一种快速、准确的选择。
{"title":"Evaluation of the GenoType NTM-DR line probe assay for nontuberculous mycobacteria using whole genome sequences as reference standard","authors":"L. Elaine Epperson , Rebecca M. Davidson , Sara M. Kammlade , Nabeeh A. Hasan , Sophie E. Nick , Iara M.P. Machado , Valerie H. Rodriguez , Aaron Appleman , Niels K. Helstrom , Michael Strong","doi":"10.1016/j.diagmicrobio.2024.116526","DOIUrl":"10.1016/j.diagmicrobio.2024.116526","url":null,"abstract":"<div><p>Pulmonary nontuberculous mycobacteria (NTM) disease is an emerging public health challenge that is especially problematic in people with cystic fibrosis (CF). Effective treatment depends on accurate species and subspecies identification and antimicrobial susceptibility status. We evaluated the GenoType NTM-DR VER 1.0 assay using biobanked NTM isolates with whole genome sequence (WGS) data and control isolates (total n=285). Species and subspecies detection sensitivity and specificity were 100 % for all species and subspecies except for two subspecies of <em>M. intracellulare,</em> that demonstrated a small degree of discrepant identification between <em>M. intracellulare</em> subspecies <em>intracellulare</em> and subspecies <em>chimaera</em>. All antimicrobial resistance markers were identified with 100 % sensitivity and specificity.</p><p>We conclude that the GenoType NTM-DR assay offers a rapid and accurate option for identifying the most frequently encountered pathogenic NTM taxa and drug resistance markers.</p><p><strong>SUPPORT:</strong> Colorado CF Research Development Program and Colorado CF National Resource Centers funded by the Cystic Fibrosis Foundation, NJH Advanced Diagnostics Laboratories, Colorado Advanced Industries Accelerator Grant</p></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"110 4","pages":"Article 116526"},"PeriodicalIF":2.1,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0732889324003523/pdfft?md5=44df0dcde095ab7ee7ea6990d39d22dd&pid=1-s2.0-S0732889324003523-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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