Diagnostic microbiology and infectious disease最新文献

{"title":"Revisiting the time to positivity in blood cultures as a prediagnostic tool: an in vitro experiment and retrospective study","authors":"Mélanie Bouillon ,&nbsp;Damasie Malandain ,&nbsp;Sylvie Dargère ,&nbsp;Vincent Cattoir ,&nbsp;Simon Le Hello ,&nbsp;François Gravey","doi":"10.1016/j.diagmicrobio.2025.117224","DOIUrl":"10.1016/j.diagmicrobio.2025.117224","url":null,"abstract":"<div><h3>Background</h3><div>Sepsis is an acute response to infection; rapid detection of pathogens is essential. In this work, the time to positivity (TTP) of blood culture (BC), a key factor for species diagnosis, was studied.</div></div><div><h3>Methods</h3><div>In the experimental phase, six bacterial reference strains were tested under varying conditions, and three years of clinical blood culture data were reviewed. A total of 4,924 first positive BC bottles from 3864 patients hospitalized from 2019 to 2021 were analysed.</div></div><div><h3>Results</h3><div>Experimental results revealed that reducing the inoculum by a factor of 10 increased the TTP by an average of 1.5 hours. A blood sample volume between 1 and 3 mL per vial significantly improved bacterial detection. Each hour of delay before incubation reduced the TTP by 21 minutes; however, the overall TTP was still prolonged. Bacterial growth and viability were preserved at room temperature for up to 24 h. TTP could be exploited as a first diagnostic tool, as it significantly varied between species. <em>Staphylococcus aureus</em> can be differentiated from coagulase-negative staphylococci when the TTP is &lt;12 hours (Specificity (Sp) = 0.95, positive predictive value (PPV) = 62 %, negative predictive value (NPV) = 76 %), and Enterobacteriaceae can be differentiated from <em>Pseudomonas aeruginosa</em> under the same threshold (Sensibility (Se) = 0.70, Sp = 0.93, PPV = 99 %). A TTP &gt;15 hours exclude clinically relevant pathogens such as <em>S. pneumoniae, S. agalactiae</em>, and <em>S. pyogenes</em>.</div></div><div><h3>Conclusion</h3><div>This work shows how preanalytical factors play a major role in the time required for diagnosis, and highlights the potential of the TTP as a prediagnostic tool to improve sepsis management.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117224"},"PeriodicalIF":1.8,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular diagnostics in clinical microbiology: Advances, applications, and future directions","authors":"Sijo Asokan ,&nbsp;Avinash Choudekar ,&nbsp;Arunkumar Jagadeesan ,&nbsp;Rudresh SM ,&nbsp;Aamir Ali ,&nbsp;Santosh Uttamrao Napte ,&nbsp;Saranya Ashokapuram Selvam ,&nbsp;Gaurav Verma ,&nbsp;Priti Das ,&nbsp;Nisha Beniwal ,&nbsp;Guhanraj Radhamanalan ,&nbsp;Smitha Vijayan ,&nbsp;Divya Rajeswary ,&nbsp;Teena Jacob","doi":"10.1016/j.diagmicrobio.2025.117223","DOIUrl":"10.1016/j.diagmicrobio.2025.117223","url":null,"abstract":"<div><div>Molecular diagnostics have transformed clinical microbiology by enabling rapid, accurate, and highly sensitive detection of infectious agents, significantly improving patient outcomes and public health response. Over the past decade, advances in polymerase chain reaction (PCR), next-generation sequencing (NGS), digital PCR, isothermal amplification, and CRISPR-based assays have enhanced pathogen identification, antimicrobial resistance profiling, and outbreak investigation. These technologies have been instrumental in detecting and monitoring emerging and re-emerging viral pathogens such as SARS-CoV-2, Nipah, Zika, H5N1 avian influenza, Mpox, Ebola, Marburg, and MERS-CoV. Molecular diagnostics also play a critical role in antimicrobial resistance surveillance and hospital infection control by enabling high-resolution tracking of resistance genes and pathogen transmission dynamics. Despite these achievements, challenges remain regarding implementation costs, technical expertise, infrastructure, and the need for global standardization. Future directions focus on developing cost-effective, point-of-care molecular platforms, integrating artificial intelligence and bioinformatics for enhanced interpretation, and applying these technologies within One Health and environmental surveillance frameworks. These innovations will be pivotal for early outbreak detection, real-time data-driven decision-making, and equitable access to advanced diagnostics worldwide. Ultimately, molecular diagnostics are poised to remain the cornerstone of precision medicine and infectious disease control in the era of global health challenges.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117223"},"PeriodicalIF":1.8,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression profiles of GPR56 and CD99 in T cells of AIDS patients and assessment of diagnostic value","authors":"Yuanzhong Sun ,&nbsp;Yuan Yuan ,&nbsp;Xueli Chen ,&nbsp;Yuemei Liang ,&nbsp;Yugui Su ,&nbsp;Liling Yang ,&nbsp;Qingyang Zhong ,&nbsp;Suidong Ouyang ,&nbsp;Xuerong Sun ,&nbsp;Minnan Ye ,&nbsp;Pingzhang Wang ,&nbsp;Wenrui Li","doi":"10.1016/j.diagmicrobio.2025.117222","DOIUrl":"10.1016/j.diagmicrobio.2025.117222","url":null,"abstract":"<div><h3>Objectives</h3><div>GPR56, an adhesion G protein-coupled receptor, and CD99, a cell-surface glycoprotein, have emerged as important immunomodulators in HIV infection. GPR56 plays a crucial role in T cell migration, activation, and the interaction between the virus and the host cell. Concurrently, CD99 is implicated in regulating key processes such as T cell adhesion, migration, and apoptotic signaling. This study aimed to investigate their dynamic expression in T-cell subsets as potential biomarkers for AIDS progression and diagnosis.</div></div><div><h3>Methods</h3><div>Differentially expressed genes in T cells were screened using single-cell RNA sequencing. Experimental validation was performed by flow cytometry on peripheral blood samples from 36 PLWH (male:31, female:5, age: 41.58±12.42) and 35 healthy controls (male:25, female:10, age: 32.17±12.09). Systematically evaluated the protein expression profiles of GPR56 and CD99 on the surface of T cells. The Pearson method assessed correlation, and receiver operating characteristic (ROC) analysis evaluated diagnostic performance.</div></div><div><h3>Results</h3><div>GPR56 expression on CD8⁺ T cells increased significantly with disease progression (<em>P</em> &lt; 0.05), while CD99 expression remained stable across stages. The CD8⁺GPR56⁺CD99⁺ T-cell subset demonstrated high diagnostic accuracy for HIV infection (AUC = 0.90), with frequencies that increased progressively with disease severity. Moderate co-expression of GPR56 and CD99 was observed in CD8⁺ T cells in both cohorts. In contrast, CD4⁺GPR56⁺ T cells showed no discriminative capacity between early-stage patients and controls.</div></div><div><h3>Conclusions</h3><div>GPR56 and CD99 exhibit distinct expression patterns during HIV infection. The CD8⁺GPR56⁺ and CD8⁺GPR56⁺CD99⁺ T-cell subsets serve as potent stage-specific biomarkers, with combined GPR56/CD99 analysis enhancing diagnostic precision for advanced AIDS.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117222"},"PeriodicalIF":1.8,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights on vaccine-derived poliovirus type 2 outbreaks and novel oral polio vaccine candidates","authors":"Khaled M. Aboshanab ,&nbsp;Mohammad Y. Alshahrani ,&nbsp;Hesham Ali El Enshasy ,&nbsp;Menna R Shabana ,&nbsp;Abu Bakar Abdul Majeed","doi":"10.1016/j.diagmicrobio.2025.117221","DOIUrl":"10.1016/j.diagmicrobio.2025.117221","url":null,"abstract":"<div><div>Despite the life-saving benefits of the vaccination with the live attenuated oral polio vaccine, or Sabin (OPV), OPV still evolves rapidly to lose attenuating determinants critical to the reacquisition of virulence, resulting in vaccine-derived, virulent poliovirus variants. The circulation of these variants within under-immunized populations leads to the further evolution of circulating, vaccine-derived poliovirus with increased transmission capacity, representing a significant risk of polio re-emergence. Although the wild poliovirus type 2 (WPV2) had been declared as eradicated worldwide in 2015, outbreaks of circulating VDPV-type 2 (cVDPV2) have been increasingly reported, especially after the tOPV-bOPV switch that occurred in 2016, which resulted in building a cohort of susceptible individuals with lower type 2 intestinal immunity. Since the response to outbreaks is done through conducting supplementary immunization activities (SIAs) using type-2 containing OPV, this carries a risk of mutations, especially with suboptimum coverage and potential further circulation. The recent detection of outbreaks of cVDPV2 in some countries necessitated the development of a genetically stable novel oral polio vaccine type 2 (nOPV-2) to decrease the chances of mutations and hence outbreaks. This review highlights the recent outbreaks of VDPV serotypes, emphasizing the role of the World Health Organization and the Global Polio Eradication Initiative in detecting and controlling VDPV outbreaks. The emerging VDPV, strategies implemented, reported clinical trials, and proposals for developing nOPV. The way the review is presented will encourage assertiveness, assist in posing open-ended questions, and provide several ideas for further research.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117221"},"PeriodicalIF":1.8,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method comparison of QIAGEN QuantiFERON-TB gold plus ELISA and LIAISON® QuantiFERON-TB gold plus for latent tuberculosis infection detection in pregnant ugandan women","authors":"Felix Bongomin ,&nbsp;Diana Sitenda ,&nbsp;Phillip Ssekamatte ,&nbsp;Bwambale Jonani ,&nbsp;Joseph Baruch Baluku ,&nbsp;Irene Andia-Biraro","doi":"10.1016/j.diagmicrobio.2025.117220","DOIUrl":"10.1016/j.diagmicrobio.2025.117220","url":null,"abstract":"<div><h3>Background</h3><div>The DiaSorin LIAISON® QuantiFERON-TB Gold Plus test offers automation advantages over the standard QIAGEN ELISA; however, data comparing the two methods in pregnant women are lacking. We assessed the categorical and quantitative agreement between these interferon-gamma release assay platforms for latent tuberculosis infection (LTBI) detection in a cohort of pregnant women in Uganda.</div></div><div><h3>Methods</h3><div>In this cross-sectional diagnostic comparison, 261 pregnant women were enrolled at Kawempe National Referral Hospital in Kampala, Uganda (September–December 2020). Plasma samples were initially tested with QIAGEN ELISA (QFT-ELISA) in 2021, stored at −80°C, and retested in 2025 with both LIAISON® chemiluminescent immunoassay (QFT-CLIA) and QFT-ELISA. Agreement was assessed using Cohen's kappa, Bland-Altman analysis, and Deming regression analyses. Stability over four years was evaluated using weighted kappa and transition analyses. Subgroup analyses examined agreement by HIV status and trimester</div></div><div><h3>Results</h3><div>Of the 261 enrolled participants, 231 samples (88.5 %) remained viable after four years storage. Overall agreement between QFT-CLIA and QFT-ELISA in 2025 was 90.5 % (Cohen's Kappa 0.82; 95 % CI: 0.75-0.89). Bland-Altman analysis showed minimal systematic bias (mean difference for TB1-Nil: +0.046 IU/mL, <em>p</em> = 0.608, TB2 Nil: +0.0776 IU/mL, <em>p</em> = 0.3175), and Deming regression confirmed linearity (slope, 0.94-0.96). Among the 187 samples that remained QFT-stable between 2021 and 2025, the agreement improved to 98.4 % (kappa 0.97; 95 % CI: 0.94-1.00), with category-specific agreement of 93.6 %, 97.5 %, and 100 % for positive, negative, and indeterminate results, respectively. Four-year stability varied according to baseline classification: 57.1 % for positive samples, 97.5 % for negative samples, and 75.0 % for indeterminate samples. Agreement was similar between HIV-positive (84.6 %, Kappa 0.76) and HIV-negative participants (90.8 %, Kappa 0.82; <em>p</em> = 0.357).</div></div><div><h3>Conclusions</h3><div>The LIAISON® platform demonstrated excellent agreement with the QIAGEN ELISA for LTBI detection during pregnancy, particularly when storage effects were minimized. Long-term cryopreservation disproportionately affects positive samples. The automation of the LIAISON® platform supports high-throughput implementation in antenatal care settings; however, fresh sample testing should be prioritized for clinical decision-making.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117220"},"PeriodicalIF":1.8,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145732962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycoplasma pneumoniae macrolide resistance acquisition in a CCR2-deficient child","authors":"Nora Poey ,&nbsp;Jennifer Guiraud ,&nbsp;Carla Balcon ,&nbsp;N. Aladjidi ,&nbsp;Marie Gardette ,&nbsp;Sabine Pereyre ,&nbsp;Francois Galode ,&nbsp;Mickael Fayon ,&nbsp;Cécile Bébéar","doi":"10.1016/j.diagmicrobio.2025.117219","DOIUrl":"10.1016/j.diagmicrobio.2025.117219","url":null,"abstract":"<div><div>Since November 2023, an increase in <em>M. pneumoniae</em> respiratory infections has been observed in France. Macrolides are the reference treatment and are indicated as first-line therapy. Macrolide-resistant strains have been described in clinical isolates, especially in Asian countries. In the present manuscript, we report the persistence of an <em>M. pneumoniae</em> strain from an immunocompromised child with CCR2 deficiency on prophylactic azithromycin treatment. Antimicrobial susceptibility testing showed acquisition of macrolide resistance after treatment with azithromycin. Molecular typing using two typing methods revealed the persistence of the same strain over time. This case highlights the need for access to rapid detection tools to determine the causative pathogen and potential antimicrobial resistance mechanisms. This is particularly important for patients with comorbidities receiving prophylactic antibiotics and require additional caution. Acquisition of resistance can lead to complications and extended hospitalisation. Higher molecular resolution would be useful for the detection of putative heteroresistance in <em>M. pneumoniae</em>.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117219"},"PeriodicalIF":1.8,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145780603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling the economic impact of different testing strategies for meningitis/encephalitis in pediatric patients from a US Hospital Perspective","authors":"Rodrigo Hasbun ,&nbsp;Kyle D. Hueth ,&nbsp;Glaucia Paranhos-Baccala ,&nbsp;Lohit Korrapati ,&nbsp;Catherine Regan ,&nbsp;Adrienne Kwok ,&nbsp;Noam Kirson","doi":"10.1016/j.diagmicrobio.2025.117218","DOIUrl":"10.1016/j.diagmicrobio.2025.117218","url":null,"abstract":"<div><div>A pediatric-focused simulation model estimated that syndromic testing of suspected meningitis/encephalitis cases would result in cost savings to US hospitals of $542-$4,818 compared with rapid diagnostic testing and off-site-only testing, through more timely pathogen identification and reductions in hospital length of stay.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117218"},"PeriodicalIF":1.8,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145862286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of aztreonam/avibactam gradient test strip and aztreonam-ceftazidime/avibactam broth disk elution method for susceptibility testing of metallo-β-lactamase positive carbapenemase-producing bacteria","authors":"Hasan Hamze ,&nbsp;Aleksandra Stefanovic ,&nbsp;Leah Gowland ,&nbsp;Patrick Tang ,&nbsp;Nancy Matic ,&nbsp;Victor Leung ,&nbsp;Gordon Ritchie ,&nbsp;Christopher F Lowe ,&nbsp;Marc G Romney ,&nbsp;Michael Payne","doi":"10.1016/j.diagmicrobio.2025.117216","DOIUrl":"10.1016/j.diagmicrobio.2025.117216","url":null,"abstract":"<div><div>Metallo-β-lactamase (MBL)-producing carbapenemase-producing Enterobacterales (CPE) present significant therapeutic challenges due to resistance to most β-lactam agents, including carbapenems. Aztreonam combined with avibactam (ATM/AVI) provides a promising treatment strategy, but standardized antimicrobial susceptibility testing (AST) methods remain limited. This study evaluated the performance of the MTS™ ATM/AVI gradient strip (Liofilchem) with the CLSI-recommended ceftazidime-avibactam plus aztreonam (CZA/ATM) broth disk elution (BDE) method in 36 CPE and 9 <em>Stenotrophomonas maltophilia</em> clinical isolates. Categorical agreement between these methods was 97.2 % for CPE and 88.9 % for <em>S. maltophilia</em>. Whole-genome sequencing was used for discrepancy analysis. Based on accuracy, reproducibility, and ease of use, the ATM/AVI gradient strip was selected as the preferred method for clinical laboratory testing.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117216"},"PeriodicalIF":1.8,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145721664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of delayed incubation on mycobacterial blood culture recovery with the BACTEC Myco/F Lytic system","authors":"Emmanuel Lecorche ,&nbsp;Typhaine Billard-Pomares ,&nbsp;Fatma Magdoud-El Alaoui ,&nbsp;Emmanuelle Gallois ,&nbsp;Vincent Fihman ,&nbsp;Amélie Carrër ,&nbsp;Laura Djamdjian ,&nbsp;François Camelena ,&nbsp;Faiza Mougari ,&nbsp;Etienne Carbonnelle ,&nbsp;Paul-Louis Woerther","doi":"10.1016/j.diagmicrobio.2025.117217","DOIUrl":"10.1016/j.diagmicrobio.2025.117217","url":null,"abstract":"<div><div>This study assessed the impact of delayed incubation on mycobacterial blood culture accuracy. We performed an experimental study and a 5-year retrospective analysis of 1592 clinical vials categorized by preincubation time. Delayed incubation for up to 3 days was not associated with lower detection rates of mycobacteria.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 3","pages":"Article 117217"},"PeriodicalIF":1.8,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145755624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover 2 - Aims/Scopes, Ed Board","authors":"","doi":"10.1016/S0732-8893(25)00521-8","DOIUrl":"10.1016/S0732-8893(25)00521-8","url":null,"abstract":"","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 2","pages":"Article 117199"},"PeriodicalIF":1.8,"publicationDate":"2025-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145690260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}