Diagnostic microbiology and infectious disease最新文献

{"title":"Discovery of parasite proteins in the urine of vivax malaria patients through mass spectrometry","authors":"Himani Tripathi ,&nbsp;Prajna Parimita Kar ,&nbsp;Araveti Prasanna Babu ,&nbsp;Anand Srivastava ,&nbsp;Geeta Pachori ,&nbsp;Tarun Kumar Bhatt","doi":"10.1016/j.diagmicrobio.2026.117281","DOIUrl":"10.1016/j.diagmicrobio.2026.117281","url":null,"abstract":"<div><div>Malaria is an infectious disease that severely affects people worldwide. The detection of its causative organism, <em>Plasmodium</em>, is mainly based on microscopy, polymerase chain reaction, and rapid diagnostic tests, all relying on blood samples. Obtaining blood samples causes pain, poses a risk of infections, and leads to poor compliance in cases of repeated sampling. This highlights the need for non-invasive diagnostic alternatives. Several studies have explored the potential use of non-blood samples, such as saliva, urine, stool, and skin to detect <em>Plasmodium</em>. Urine accumulates changes from the body and thus is an appropriate source for biomarker discovery. A biomarker is a measurable change in the body that is associated with a disease or condition. In this study, the urine samples of <em>Plasmodium vivax</em> infected patients were analyzed through mass spectrometry. A comparison of the infected and control samples revealed several proteins present in one group but absent in the other, with some proteins showing altered levels. A total of 252 human proteins were found in the infected samples only. Gene ontology analysis and functional annotation revealed that these proteins are involved in key biological pathways and processes in both the human host and the parasite, and their presence in urine samples could aid in a deeper understanding of malaria biology. Twenty-eight <em>P. vivax</em> proteins were found in the mass spectrometric analysis of urine samples, including rifin-like protein, single-stranded DNA-binding protein, and profilin. These might serve as potential biomarkers for the non-invasive detection of <em>Plasmodium</em> infection.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"115 1","pages":"Article 117281"},"PeriodicalIF":1.8,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146046303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of a duplex real-time multienzyme isothermal rapid amplification assay for simultaneous detection of hypervirulent Klebsiella pneumoniae K1 and K2 serotypes in spiked blood samples","authors":"Zhixiong Duan ,&nbsp;Shaohong Yu ,&nbsp;Honglin Wang ,&nbsp;Chunyan Yang ,&nbsp;Xuelian Peng ,&nbsp;Shan Shi ,&nbsp;Jin Li","doi":"10.1016/j.diagmicrobio.2026.117280","DOIUrl":"10.1016/j.diagmicrobio.2026.117280","url":null,"abstract":"<div><div>The emergence of hypervirulent <em>Klebsiella pneumoniae</em> (hvKP), particularly the K1 and K2 serotypes, presents a significant public health threat due to their increased virulence and resistance. Rapid and accurate detection of hvKP is crucial for effective clinical management. This study developed and evaluated a duplex real-time multienzyme isothermal rapid amplification (MIRA) assay for the simultaneous detection of hvKP K1 and K2 serotypes in spiked blood samples. The assay employed optimized primers and probes targeting specific capsular genes. Sensitivity and specificity were assessed using spiked blood specimens and compared to real-time PCR. The detection limit was 1 × 10³ CFU per reaction for both serotypes, with no cross-reactivity with non-hvKP K1/K2 strains. The assay demonstrated superior reproducibility and stability, offering faster detection and simplified infrastructure requirements compared to real-time PCR. These features make the duplex real-time MIRA assay a promising tool for clinical diagnostics and outbreak surveillance.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117280"},"PeriodicalIF":1.8,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-world laboratory functionality requirements and implementation considerations for fast phenotypic antimicrobial susceptibility testing","authors":"Patrick M. McDaneld ,&nbsp;Theresa Okeyo Owuor ,&nbsp;Shivaramu Keelara ,&nbsp;Carrie E. Lasky ,&nbsp;Andrea M. Prinzi","doi":"10.1016/j.diagmicrobio.2026.117279","DOIUrl":"10.1016/j.diagmicrobio.2026.117279","url":null,"abstract":"<div><div>Timely and effective antimicrobial therapy is essential for managing bacteremia and reducing associated morbidity and mortality. Fast phenotypic antimicrobial susceptibility testing (fAST) technologies offer the potential to accelerate optimal therapy by providing fast, actionable results. However, real-world adoption of fAST is influenced by laboratory infrastructure, antimicrobial stewardship practices, and the adaptability of new diagnostic platforms. We conducted an Early Evaluation Program (EEP) of the VITEK® REVEAL™ system at 13 U.S. hospital-based microbiology laboratories to assess performance and user experience related to the assay and fAST in general. A survey was performed as a component of the EEP to qualitatively assess barriers and facilitators to fAST implementation. Survey responses highlighted that ease of use, improved turnaround time, and clinical impact were the most valued features of fAST. Willingness to adopt fAST was high, but hesitations centered on evidence gaps, resource constraints, and the need for robust performance data. Key determinants of adoption included patient complexity, laboratory staffing, and workflow integration. Mapping responses to the Consolidated Framework for Implementation Research (CFIR) identified critical domains such as evidence base, adaptability, cost, and communication pathways. Successful implementation strategies included stakeholder education and engagement, consensus-building, and tailoring software to local needs. These findings highlight that while fAST can transform bacteremia management, its impact depends on addressing local barriers, fostering multidisciplinary collaboration, and ensuring ongoing evaluation of clinical and operational outcomes.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117279"},"PeriodicalIF":1.8,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146009304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High throughput HPV genotyping by next generation sequencing for detection of 28 HPV types and 13 sexually transmitted infections: A first community-based cervical cancer screening study from India","authors":"Yasasve Madhavan,&nbsp;Vasanthkumar Muthukumar,&nbsp;Srinidhi Ramasubramanian,&nbsp;Vijayalakshmi Ramshankar","doi":"10.1016/j.diagmicrobio.2026.117278","DOIUrl":"10.1016/j.diagmicrobio.2026.117278","url":null,"abstract":"<div><h3>Background</h3><div>High-risk human papillomavirus (HR-HPV) infection is the primary cause of cervical cancer. However, conventional PCR-based assays provide limited HPV genotyping, and cytology alone has low sensitivity for detecting subclinical infections. Comprehensive molecular approaches are needed to better characterize HPV genotypes and co-infections in community screening settings.</div></div><div><h3>Methods</h3><div>This study evaluated the analytical performance of a next-generation sequencing (NGS)–based HPV–STI assay in comparison with the clinically validated Roche Cobas^Ⓡ 4800 platform in a community screening cohort from South India. Cervical exfoliated cell samples from 96 asymptomatic, married women previously tested by Cobas^Ⓡ 4800 with partial HR-HPV genotyping (HPV16, HPV18, and a pooled group of 12 other high-risk types) were analyzed using NGS. The NGS assay enabled comprehensive detection of 28 HPV genotypes and 13 sexually transmitted infections (STIs) on the Illumina NextSeq 550 platform. Liquid-based cytology with RAPID-Pap staining was performed for all sequenced samples.</div></div><div><h3>Results</h3><div>The NGS assay showed high agreement with the Cobas^Ⓡ 4800 platform, with an overall concordance of 95.8% (92/96). Concordance was 94.4% (17/18) for HPV16 detection, 100% (3/3) for HPV18, 96.7% (58/60) for pooled other high-risk HPV types (non-HPV16/18), and 85.7% (7/8) for mixed infections, including co-infections of HPV16 or HPV18 with non-HPV16/18 high-risk types as well as HPV16/18 dual infections. Among women with abnormal cytology (ASCUS and HSIL), non-HPV16/18 infections were identified in 58.3% (7/12) of cases. Concurrent non-HPV STIs were detected in 52.1% (50/96) of participants, with <em>Ureaplasma parvum</em> and <em>Mycoplasma hominis</em> being the most prevalent.</div></div><div><h3>Conclusion</h3><div>A substantial proportion of women with abnormal cytology in this community cohort harboured non-HPV16 and HPV18 high-risk infections, highlighting the importance of extended HPV genotyping. NGS-based HPV genotyping was concordant with the comparator assay used and had simultaneous STI identification, supporting its potential utility as a comprehensive tool for community-based cervical cancer screening and risk stratification.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117278"},"PeriodicalIF":1.8,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From pandemic influenza to novel coronaviruses: emerging infectious diseases of the 21st century","authors":"Sijo Asokan ,&nbsp;Isiaka Ismaila Damilare ,&nbsp;Sunil Kumar ,&nbsp;Rohan Kumar Pandey ,&nbsp;Gaurav Verma ,&nbsp;Nilotpal Banerjee ,&nbsp;Guhanraj Radhamanalan ,&nbsp;Smitha Vijayan ,&nbsp;Teena Jacob ,&nbsp;Divya Rajeswary","doi":"10.1016/j.diagmicrobio.2026.117277","DOIUrl":"10.1016/j.diagmicrobio.2026.117277","url":null,"abstract":"<div><div>Emerging infectious diseases have risen significantly in the twenty-first century as ecological disruption, climate change, expanding human–animal interfaces, and global mobility intensify opportunities for pathogen transmission. This review synthesizes historical and contemporary evidence across viral, bacterial, fungal, and parasitic threats to characterize how diverse pathogens emerge and spread. Foundational events such as the 1918 influenza pandemic, mid-century influenza pandemics, the emergence of HIV/AIDS, and the eradication of smallpox provide context for understanding modern disease dynamics. In recent decades, coronaviruses including SARS, MERS, and SARS-CoV-2, pandemic H1N1, avian influenza subtypes, and major arboviruses such as dengue, chikungunya, Zika, West Nile virus, and yellow fever have demonstrated the rapidity with which zoonotic pathogens can disseminate globally. Viral hemorrhagic fevers including Ebola, Marburg, Lassa, and Crimean–Congo hemorrhagic fever remain critical threats, especially in regions with limited health-care capacity. Concurrently, antimicrobial resistance, the emergence of <em>Candida auris</em>, and the climate-driven expansion of endemic mycoses involving <em>Histoplasma, Coccidioides</em>, and <em>Blastomyces</em> highlight the increasing importance of fungal pathogens. Parasitic diseases such as artemisinin-resistant malaria, zoonotic trypanosomiasis, and expanding Leishmania transmission reflect shifting ecological conditions. These patterns are shaped by intersecting drivers including deforestation, wildlife trade, agricultural intensification, urban crowding, conflict, and rapid microbial evolution that enable spillover and sustained transmission. Although advances in genomic surveillance, metagenomic diagnostics, mRNA vaccines, monoclonal antibodies, and broad-spectrum antivirals have strengthened global response capacity, substantial gaps persist in equity, surveillance, and access to countermeasures. Strengthening One Health systems and resilient public health infrastructures is essential to anticipate and mitigate emerging infectious threats.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117277"},"PeriodicalIF":1.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146017815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a superspectrum for rapid identification of opportunistic human pathogens belonging to the genus Phytobacter using whole-cell MALDI-TOF MS","authors":"Alana Mazzetti ,&nbsp;Helena R.S. D’Espindula ,&nbsp;Debora O. Kulek ,&nbsp;Karen L. Jones ,&nbsp;Leticia Kraft ,&nbsp;Theo H.M. Smits ,&nbsp;Fabio Rezzonico ,&nbsp;Marcelo T. Mira ,&nbsp;Marcelo Pillonetto","doi":"10.1016/j.diagmicrobio.2026.117275","DOIUrl":"10.1016/j.diagmicrobio.2026.117275","url":null,"abstract":"<div><h3>Background</h3><div>Members of the genus <em>Phytobacter</em>, part of the <em>Enterobacteriaceae</em>, represent an emerging clinical threat. Standard identification methodologies often fail to accurately identify members of this genus because they are absent from current clinical databases. Molecular techniques are thus pivotal for differentiating <em>Phytobacter</em> from similar genera such as <em>Pantoea, Kluyvera</em>, and <em>Kosakonia</em>. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) provides a rapid, reliable, and cost-effective method for identification.</div></div><div><h3>Methods</h3><div>A <em>Phytobacter</em>-specific SuperSpectrum was constructed from 18 well-characterized isolates using standardized culture conditions, protein extraction, and triplicate MALDI-TOF MS acquisition. Only conserved, high-quality peaks were retained in the final model. Its performance was evaluated using 282 retrospective isolates and 23 prospective <em>Phytobacter</em>-suspect isolates. All <em>Phytobacte</em>r identifications, along with 154 non-<em>Phytobacter</em> identifications, were benchmarked against API 20E biochemical testing, VITEK-2 automated identification, MALDI-TOF MS current database for <em>in vitro</em> diagnostic analysis, 16S rRNA gene sequencing, and whole-genome sequencing (WGS).</div></div><div><h3>Results</h3><div>Spectral clustering showed consistent separation of <em>Phytobacter</em> at the genus level. Integration of the new SuperSpectrum into the MALDI-TOF MS SARAMIS database enabled accurate identification, leading to the identification of two retrospective <em>Phytobacter</em> out of 282 <em>Enterobacterales</em> isolates. Finally, prospective testing of 23 contemporary isolates suspected to belong to <em>Phytobacter</em> confirmed their identification against the new reference spectra, achieving 100% concordance with WGS identification, demonstrating the reliability of the updated database for genus-level identification.</div></div><div><h3>Conclusion</h3><div><em>Phytobacter</em> identification by MALDI-TOF MS provides a rapid alternative method for molecular identification of this emerging pathogen.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"115 1","pages":"Article 117275"},"PeriodicalIF":1.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Achieving 24-hour reporting turnaround for clinical surveillance detection of eight carbapenemase genes using combined multiplex molecular assays","authors":"Majie C. Foster,&nbsp;Kailee Cummings,&nbsp;Mohammad Y. Khan,&nbsp;Janine Bodnar,&nbsp;Christine Jacobsen,&nbsp;Kara Miller,&nbsp;Nicola Faraci,&nbsp;Shannon Morris,&nbsp;Catharine Prussing,&nbsp;Elizabeth Nazarian,&nbsp;Kimberlee A. Musser","doi":"10.1016/j.diagmicrobio.2026.117273","DOIUrl":"10.1016/j.diagmicrobio.2026.117273","url":null,"abstract":"<div><div>Carbapenemase-producing organisms, including carbapenem-resistant Enterobacterales, <em>Acinetobacter</em> and <em>Pseudomonas</em> species, contribute to over 49,000 infections and &gt;4,000 deaths annually in the United States. The Centers for Disease Control and Prevention recommends surveillance using dual swab testing.</div><div>While a culture-first approach is routinely utilized for carbapenemase-producing organism screening, results require 3 workdays or more. In contrast, Wadsworth Center employs a molecular-first algorithm, combining the Cepheid Xpert Carba-R assay with laboratory developed test multiplex PCR. Using shared reagents for efficiency and specimen preservation, this method detects the most prevalent carbapenemase genes, <em>bla</em>_KPC, <em>bla</em>_NDM, <em>bla</em>_VIM, <em>bla</em>_OXA-48-like, and <em>bla</em>_IMP, as well as <em>Acinetobacter baumannii</em>-associated genes, <em>bla</em>_OXA-23-like, <em>bla</em>_{OXA-24/40-like}, and <em>bla</em>_OXA-58-like, and produces actionable results in less than one workday. Wadsworth Center’s rapid carbapenemase-producing organism colonization screening algorithm supports prompt healthcare protocol initiation and epidemiological response.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117273"},"PeriodicalIF":1.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perinephritic and psoas abscess by an unusual coinfection with Trichomonas vaginalis and Lactobacillus johnsonii","authors":"Min Quan ,&nbsp;Xiaoxia Zhang ,&nbsp;Chao Chen ,&nbsp;Yu Feng ,&nbsp;Xiaoju Lv ,&nbsp;Xiaohui Wang ,&nbsp;Hui Ye","doi":"10.1016/j.diagmicrobio.2026.117276","DOIUrl":"10.1016/j.diagmicrobio.2026.117276","url":null,"abstract":"<div><div>Trichomoniasis, caused by <em>Trichomonas vaginalis,</em> is a common nonviral sexually transmitted infection that presents with vaginitis, urethritis, cystitis, prostatitis, and rarely perinephric abscess. Here we presented a 49-year-old female with fever and lumbago diagnosed with a coinfection of perinephric and psoas abscess caused by <em>Lactobacillus johnsonii</em> and <em>T. vaginalis</em>, with the etiological diagnosis established using pus culture and metagenomic next-generation sequencing (mNGS)<em>.</em> The patient achieved complete recovery following abscess drainage, incision, and combined therapy with metronidazole and piperacillin/tazobactam. Rare manifestations of trichomoniasis are easily misdiagnosed, and mNGS can help identify <em>T. vaginalis</em> quickly and accurately without prediction.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117276"},"PeriodicalIF":1.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eighteen-year laboratory-based surveillance of human coronavirus OC43 in a single tertiary hospital in the Republic of Korea: Temporal inflection, seasonal stability, and age-dependent risk","authors":"Mi-Ru Oh ,&nbsp;Ga-Yeon Kim ,&nbsp;Jeong Su Han ,&nbsp;Jae Kyung Kim","doi":"10.1016/j.diagmicrobio.2026.117274","DOIUrl":"10.1016/j.diagmicrobio.2026.117274","url":null,"abstract":"<div><div>Human coronavirus OC43 (HCoV-OC43), a member of the betacoronavirus genus, is a seasonally circulating respiratory virus that persists globally; however, long-term laboratory-based surveillance data remain limited. We analyzed multiplex real-time PCR test results collected at Dankook University Hospital between 2007 and 2024 to comprehensively evaluate long-term annual trends, seasonal structure, and age-specific detection patterns of HCoV-OC43.</div><div>Based on nasopharyngeal swab specimens, the annual positivity rate of HCoV-OC43 ranged from 0.16% to 3.05%. A marked suppression in detection was observed during the COVID-19 pandemic (2020–2021) compared with the pre-pandemic years. This was followed by a partial recovery in 2022–2023; however, detection declined again in 2024 without fully returning to pre-pandemic levels. Seasonal analysis demonstrated a consistent winter predominance throughout the study, with the highest positivity observed in winter (4.44%) and the lowest in summer (1.16%), despite interannual variability. Age-specific analysis revealed highest positivity rates in early childhood 1–4 years (3.12%) and among infants aged 0 years (2.71%), followed by a progressive decline in detection across school-age children, adolescents, adults, and older adults, indicating a clear age-dependent distribution. These findings provide laboratory-based evidence characterizing the temporal, seasonal, and age-related detection patterns of HCoV-OC43 using long-term diagnostic data from a single institution. This information may serve as a reference for interpreting routine respiratory virus testing results and understanding seasonal diagnostic demand and surveillance strategies in clinical practice.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117274"},"PeriodicalIF":1.8,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and phylogenetic analysis of KI polyomavirus and WU polyomavirus in respiratory tract samples from children under five","authors":"Hanife Tutan ,&nbsp;Yeşim Tok ,&nbsp;Mert Ahmet Kuşkucu","doi":"10.1016/j.diagmicrobio.2026.117272","DOIUrl":"10.1016/j.diagmicrobio.2026.117272","url":null,"abstract":"<div><h3>Background and Aim</h3><div>We aimed to investigate the frequency of KI and WU polyomavirus in respiratory tract samples, the affected groups by these viruses and their clinical characteristics. Although the viruses had been discovered in 2007, their pathogenicity and virulence have not been fully elucidated yet.</div></div><div><h3>Methods</h3><div>We examined 182 nasopharyngeal aspirate/swab samples obtained pediatric patients younger than five years and sent to our laboratory between 2016 and 2019. The viruses were investigated by nested-polymerase chain reaction using primers targeting partial VP1 gene regions. The positive samples were sequenced using Sanger method for verification and compare the genetic distances between partially amplified gene regions.</div></div><div><h3>Results</h3><div>KIPyV was detected in seven (3.8%) of 182 samples and WUPyV in two (1.1%). Except one KIPyV positive patient, all positive samples belonged to patients &lt;2 years of age. All positive patients were coinfected mainly by rhinovirus and parainfluenza virus 3. Four of the patients positive for KIPyV had haematologic/oncologic diseases or other immunodeficiency conditions. While all of the sequences of KIPyV isolates were 100% similar, sequences of WUPyV isolates differed at 4 nucleotide positions.</div></div><div><h3>Conclusion</h3><div>According to the results of our study, the frequency of KIPyV and WUPyV infections is low, simultaneous respiratory tract infections caused by other viral or bacterial pathogens, immunosuppression appears to be a predisposing factors for the KIPyV and WUPyV infections. The partially amplified gene regions were highly conserved in both viruses.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"114 4","pages":"Article 117272"},"PeriodicalIF":1.8,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}