Sergey A. Fedorov, S. A. Zhurko, V. Pichugin, Vladimir A. Chiginev, Anton L. Maximov, Sanika V. Kulkarni
INTRODUCTION: Venous thromboembolic complications occupy one of the central positions in the structure of complications associated with CoronaVirus Disease 2019 (COVID-19). Characterized by high epidemiological threshold values, as well as an atypical clinical picture, they determine the need for new approaches to early diagnosis and active treatment. Surgical treatment of pulmonary embolism in itself is an extremely controversial method of reperfusion of the pulmonary arterial bed. Concerning pulmonary embolism associated with a new coronavirus infection, we did not find a single clinical case reported in the available literature. The article considers a clinical case of surgical treatment of pulmonary embolism against the background COVID-19 infection, demonstrates a comprehensive approach to treatment and describes peculiarities of the clinical picture. CONCLUSION: Based on the presented case, one should note the importance of the anticoagulant therapy in a group of people with a virus-specific intervention to prevent recurrence of thromboembolic complications. Complex surgical treatment in combination with the proposed methods of anesthesiological support are the methods of choice in a group of people at intermediate-high and high risk of early cardiac death.
{"title":"Surgical Treatment of Pulmonary Embolism Associated with COVID-19: First-Hand Experience","authors":"Sergey A. Fedorov, S. A. Zhurko, V. Pichugin, Vladimir A. Chiginev, Anton L. Maximov, Sanika V. Kulkarni","doi":"10.17816/pavlovj109916","DOIUrl":"https://doi.org/10.17816/pavlovj109916","url":null,"abstract":"INTRODUCTION: Venous thromboembolic complications occupy one of the central positions in the structure of complications associated with CoronaVirus Disease 2019 (COVID-19). Characterized by high epidemiological threshold values, as well as an atypical clinical picture, they determine the need for new approaches to early diagnosis and active treatment. Surgical treatment of pulmonary embolism in itself is an extremely controversial method of reperfusion of the pulmonary arterial bed. Concerning pulmonary embolism associated with a new coronavirus infection, we did not find a single clinical case reported in the available literature. The article considers a clinical case of surgical treatment of pulmonary embolism against the background COVID-19 infection, demonstrates a comprehensive approach to treatment and describes peculiarities of the clinical picture. \u0000CONCLUSION: Based on the presented case, one should note the importance of the anticoagulant therapy in a group of people with a virus-specific intervention to prevent recurrence of thromboembolic complications. Complex surgical treatment in combination with the proposed methods of anesthesiological support are the methods of choice in a group of people at intermediate-high and high risk of early cardiac death.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133668279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Swati Hagone, Patil U. Bharat, Anupama Gupta, Gangane Nitin
BACKGROUND: Forkhead box P3 protein (FOXP3) is expressed in both tumor cells and tumor-infiltrating lymphocytes (TILs) and is reported to be associated with differences in clinical outcomes. Recent literature shows that FOXP3 positive (FOXP3+) T regulator cells (Tregs) influence anti-tumor immunity in solid tumors. АIM: To explore FOXP3+ Tregs expression related to various prognostic factors in breast carcinoma (BC) in the central Indian population. Our study is also helpful in correlating the role of FOXP3+ Tregs in the survival of invasive ductal BC with different histopathological presentations. MATERIALS AND METHODS: This is a retrospective and prospective observational study in which FOXP3+ Tregs was counted in the peritumoral area by immunohistochemistry in 47 consecutive cases of BC operated on and diagnosed already. The patients were followed for 48 to 69 months for disease progression. RESULTS: High-grade tumors are prevalent (n = 30) in the study area irrespective of the stage of clinical presentation. Patients who could adhere to their treatment plan remained free of adverse outcomes until the end of our follow-up period of 69 months (p = 0.001). No molecular subtype in our study showed specific predilection towards a high Tregs count in the peri-tumoral area. No other clinical or pathological parameters significantly correlated with FOXP3 +Treg count, including overall survival and disease-free survival. CONCLUSION: The study shows that luminal human epidermal growth factor receptor 2 negativ and human epidermal growth factor receptor 2 enriched BC show comparatively high FOXP3+ Tregs count. There is no relation with tumor grade, TNM stage, important immune markers, or overall survival and disease-free survival.
{"title":"Expression of FOXP3 of Tumor-Infiltrating Lymphocytes in Invasive Breast Cancer: Its Relationship to Histopathological Parameters and Overall Survival","authors":"Swati Hagone, Patil U. Bharat, Anupama Gupta, Gangane Nitin","doi":"10.17816/pavlovj108478","DOIUrl":"https://doi.org/10.17816/pavlovj108478","url":null,"abstract":"BACKGROUND: Forkhead box P3 protein (FOXP3) is expressed in both tumor cells and tumor-infiltrating lymphocytes (TILs) and is reported to be associated with differences in clinical outcomes. Recent literature shows that FOXP3 positive (FOXP3+) T regulator cells (Tregs) influence anti-tumor immunity in solid tumors. \u0000АIM: To explore FOXP3+ Tregs expression related to various prognostic factors in breast carcinoma (BC) in the central Indian population. Our study is also helpful in correlating the role of FOXP3+ Tregs in the survival of invasive ductal BC with different histopathological presentations. \u0000MATERIALS AND METHODS: This is a retrospective and prospective observational study in which FOXP3+ Tregs was counted in the peritumoral area by immunohistochemistry in 47 consecutive cases of BC operated on and diagnosed already. The patients were followed for 48 to 69 months for disease progression. \u0000RESULTS: High-grade tumors are prevalent (n = 30) in the study area irrespective of the stage of clinical presentation. Patients who could adhere to their treatment plan remained free of adverse outcomes until the end of our follow-up period of 69 months (p = 0.001). No molecular subtype in our study showed specific predilection towards a high Tregs count in the peri-tumoral area. No other clinical or pathological parameters significantly correlated with FOXP3 +Treg count, including overall survival and disease-free survival. \u0000CONCLUSION: The study shows that luminal human epidermal growth factor receptor 2 negativ and human epidermal growth factor receptor 2 enriched BC show comparatively high FOXP3+ Tregs count. There is no relation with tumor grade, TNM stage, important immune markers, or overall survival and disease-free survival.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"130 7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114168403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Irina V. Lopukhova, A. Korolev, E. Nikitenko, E. Denisova, E. Kirpichenkova, E. Fanda, E. Petrova
INTRODUCTION: A balanced diet provides the optimal level of metabolism and is one of key modifiable factors of the primary prophylaxis of chronic non-infectious diseases. Excess of the common fat in the diet is the most common alimentary imbalance in nutrition of the population. AIM: A comparative characteristics of the level of the alimentary intake and of the main alimentary sources of lipid components in medical university students. MATERIALS AND METHODS: For quantification of entry of lipid components, the method of triple 24-hour reproduction was used. The data on actual nutrition were acquired and analyzed using a specialized questionnaire developed for the given study. RESULTS: A comparative analysis of levels of intake of the common fat, saturated and polyunsaturated fatty acids did not identify any statistically significant differences in women and men in both groups the main sources of the common fat and saturated fatty acids were hard cheese, milk chocolate, sausage products, chicken eggs, butter and mayonnaise. The main contribution to the required level of polyunsaturated fatty acids, irrespective of gender, were made by sunflower oil, fish, mayonnaise, nuts, seeds, pizza and olive oil. CONCLUSION: The level of intake of common fat with the diet exceeds the physiological demand in 95.3% of women and 94.8% of men. A share of saturated fatty acids made more than 10% of the energy value in 92% of female respondents and in 94% of male respondents, which is associated with the excessive quantity of the sources of hidden fat in the diet of students of the two comparison groups, such as hard cheese, milk chocolate and sausage products. In 45% of students, owing to the presence of oils, fish, mayonnaise and nuts in their diet, the level of entry of polyunsaturated fatty acids met the physiological demand.
{"title":"A comparative Hygienic Evaluation of Balance of Lipid Components in the Diet of Medical University Students","authors":"Irina V. Lopukhova, A. Korolev, E. Nikitenko, E. Denisova, E. Kirpichenkova, E. Fanda, E. Petrova","doi":"10.17816/pavlovj111844","DOIUrl":"https://doi.org/10.17816/pavlovj111844","url":null,"abstract":"INTRODUCTION: A balanced diet provides the optimal level of metabolism and is one of key modifiable factors of the primary prophylaxis of chronic non-infectious diseases. Excess of the common fat in the diet is the most common alimentary imbalance in nutrition of the population. \u0000AIM: A comparative characteristics of the level of the alimentary intake and of the main alimentary sources of lipid components in medical university students. \u0000MATERIALS AND METHODS: For quantification of entry of lipid components, the method of triple 24-hour reproduction was used. The data on actual nutrition were acquired and analyzed using a specialized questionnaire developed for the given study. \u0000RESULTS: A comparative analysis of levels of intake of the common fat, saturated and polyunsaturated fatty acids did not identify any statistically significant differences in women and men in both groups the main sources of the common fat and saturated fatty acids were hard cheese, milk chocolate, sausage products, chicken eggs, butter and mayonnaise. The main contribution to the required level of polyunsaturated fatty acids, irrespective of gender, were made by sunflower oil, fish, mayonnaise, nuts, seeds, pizza and olive oil. \u0000CONCLUSION: The level of intake of common fat with the diet exceeds the physiological demand in 95.3% of women and 94.8% of men. A share of saturated fatty acids made more than 10% of the energy value in 92% of female respondents and in 94% of male respondents, which is associated with the excessive quantity of the sources of hidden fat in the diet of students of the two comparison groups, such as hard cheese, milk chocolate and sausage products. In 45% of students, owing to the presence of oils, fish, mayonnaise and nuts in their diet, the level of entry of polyunsaturated fatty acids met the physiological demand.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127641448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Murtazina, I. I. Korobeynikova, L. Poskotinova, N. Karatygin, S. Pertsov
INTRODUCTION: The aim of the work is analysis of one of the most important problems of the modern biomedical science problem of studying the processes of human adaptation to a complex of climatic and geographic factors and conditions of the Far North in the period of increased necessity in realization of the economic, industrial and defense activity of the government. The review presents the data of scientific study of cognitive functions and neurophysiological changes in people permanently living in or arriving to the Arctic zone. In the first part, the factors are considered that evidence different disorders in memory, concentration, efficiency of performing simple and complex cognitive tasks by test persons in conditions of low temperature, seasonal photoperiodicity, a particular working regime, such as rotational team method, etc. The age-related peculiarities of dynamics of the cognitive processes in children and adolescents living in the Arctic region are considered. The second part of the article presents analysis of scientific data on changes of electrophysiological parameters of the brain structure activity under the influence of individual or complex factors of Arctic conditions. In particular, changes of the encephalographic rhythms, evoked potentials, hemispheric asymmetry and parameters of autonomic regulation of the heart rate variability in the initial condition and on exposure to the factors of the Far North, are described. A necessity of individual typological analysis of the adaptation process characteristics depending on the initial regulatory peculiarities, neurophysiological characteristics of people, duration of their stay in the mentioned conditions, is emphasized. Inconsistency or ambiguity of the presented data indicate the importance of using a complex approach to scientific research which should include systemic analysis of the dynamics of neurophysiological characteristics and parameters of achieved effectiveness of modeled or professional activity in the Arctic conditions. CONCLUSION: The paper substantiates the necessity of elaboration of personalized methods of increasing the adaptive reserves of people working and living in the Arctic zones; one of these may be modern systems based on biocontrol with feedback from physiological systems of a human. Besides, it is noted that application of the principles of the theory of the functional systems formulated by academician P. K. Anokhin, can contribute to deeper understanding of adaptation processes, changes of cognitive and professional abilities, their neurophysiological support in the Arctic conditions.
{"title":"Analysis of Cognitive Functions and Neurophysiological Processes in Adaptation of Human to Conditions of the Arctic Region","authors":"E. Murtazina, I. I. Korobeynikova, L. Poskotinova, N. Karatygin, S. Pertsov","doi":"10.17816/pavlovj109581","DOIUrl":"https://doi.org/10.17816/pavlovj109581","url":null,"abstract":"INTRODUCTION: The aim of the work is analysis of one of the most important problems of the modern biomedical science problem of studying the processes of human adaptation to a complex of climatic and geographic factors and conditions of the Far North in the period of increased necessity in realization of the economic, industrial and defense activity of the government. The review presents the data of scientific study of cognitive functions and neurophysiological changes in people permanently living in or arriving to the Arctic zone. In the first part, the factors are considered that evidence different disorders in memory, concentration, efficiency of performing simple and complex cognitive tasks by test persons in conditions of low temperature, seasonal photoperiodicity, a particular working regime, such as rotational team method, etc. The age-related peculiarities of dynamics of the cognitive processes in children and adolescents living in the Arctic region are considered. The second part of the article presents analysis of scientific data on changes of electrophysiological parameters of the brain structure activity under the influence of individual or complex factors of Arctic conditions. In particular, changes of the encephalographic rhythms, evoked potentials, hemispheric asymmetry and parameters of autonomic regulation of the heart rate variability in the initial condition and on exposure to the factors of the Far North, are described. A necessity of individual typological analysis of the adaptation process characteristics depending on the initial regulatory peculiarities, neurophysiological characteristics of people, duration of their stay in the mentioned conditions, is emphasized. Inconsistency or ambiguity of the presented data indicate the importance of using a complex approach to scientific research which should include systemic analysis of the dynamics of neurophysiological characteristics and parameters of achieved effectiveness of modeled or professional activity in the Arctic conditions. \u0000CONCLUSION: The paper substantiates the necessity of elaboration of personalized methods of increasing the adaptive reserves of people working and living in the Arctic zones; one of these may be modern systems based on biocontrol with feedback from physiological systems of a human. Besides, it is noted that application of the principles of the theory of the functional systems formulated by academician P. K. Anokhin, can contribute to deeper understanding of adaptation processes, changes of cognitive and professional abilities, their neurophysiological support in the Arctic conditions.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132300209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aleksandr R. Ambartsumyan, B. A. Chumak, L. Deryagina, Sergey S. Batskov, E. D. Pyatibrat
INTRODUCTION: Despite the technical advance in medicine and the use of minimally invasive laparoscopic techniques, complications of cholecystectomy considerably reduce the quality of life of the operated patients. Development of the most common and severe complication fatty liver disease (FLD) is difficult to predict due to numerous factors that influence its formation. AIM: To identify prognostic criteria of formation of FLD in the long-term period after cholecystectomy and to scientifically substantiate their significance. MATERIALS AND METHODS: To identify prognostic criteria of formation of FLD in the long-term period after minimally invasive cholecystectomy, 330 patients (159 men and 171 women) who underwent surgical treatment, were examined. Anthropometric data, hemodynamic parameters were evaluated, autonomic index was calculated. US evaluation of choledoch and Elastography of the liver, magnetic resonance tomography of the liver and magnetic resonance cholangiopancreatography were performed. Using chromatography-mass-spectrometry, microbial markers of parietal microbiome of the intestine were determined. RESULTS: Parameters of body mass index indicate the interrelation between the formation of FLD and increased body mass in patients of older age. In the first group with FLD, sympathetic status prevailed, and in the control group of the same age, parasympathicotonia was predominating. At the older age, in the second and third subgroup, the autonomic regulation did not influence the formation of FLD. The choledoch diameter in men and women with FLD of the first age subgroup was greater relative to patients of the control group and of the preoperative period and also relative to older patients of the same group. In the long-term period after cholecystectomy in the group with fatty liver disease, the opportunistic microflora prevailed over essential one, and aerobic microflora over anaerobic one. Besides, increased amount of fungi and viruses was noted, as well as a change in the distribution of obligate microflora due to decrease in the content of eubacteria and predomination of lactobacteria over bifidobacteria in the parietal layer of the intestine. In the individuals of older age, reduction of the obligate and increase in the opportunistic microflora including aerobic one, was noted. CONCLUSIONS: Age, autonomic regulation and body mass have a significant influence on the formation of FLD in the long-term period after cholecystectomy. In the group of older patients, the influence of the autonomic regulation is lower, and increase in body mass is higher than in younger individuals.
{"title":"Criteria of Formation of Fatty Liver Disease in Individuals of Different Age Groups in the Long-Term Period after Minimally Invasive Cholecystectomy","authors":"Aleksandr R. Ambartsumyan, B. A. Chumak, L. Deryagina, Sergey S. Batskov, E. D. Pyatibrat","doi":"10.17816/pavlovj110948","DOIUrl":"https://doi.org/10.17816/pavlovj110948","url":null,"abstract":"INTRODUCTION: Despite the technical advance in medicine and the use of minimally invasive laparoscopic techniques, complications of cholecystectomy considerably reduce the quality of life of the operated patients. Development of the most common and severe complication fatty liver disease (FLD) is difficult to predict due to numerous factors that influence its formation. \u0000AIM: To identify prognostic criteria of formation of FLD in the long-term period after cholecystectomy and to scientifically substantiate their significance. \u0000MATERIALS AND METHODS: To identify prognostic criteria of formation of FLD in the long-term period after minimally invasive cholecystectomy, 330 patients (159 men and 171 women) who underwent surgical treatment, were examined. Anthropometric data, hemodynamic parameters were evaluated, autonomic index was calculated. US evaluation of choledoch and Elastography of the liver, magnetic resonance tomography of the liver and magnetic resonance cholangiopancreatography were performed. Using chromatography-mass-spectrometry, microbial markers of parietal microbiome of the intestine were determined. \u0000RESULTS: Parameters of body mass index indicate the interrelation between the formation of FLD and increased body mass in patients of older age. In the first group with FLD, sympathetic status prevailed, and in the control group of the same age, parasympathicotonia was predominating. At the older age, in the second and third subgroup, the autonomic regulation did not influence the formation of FLD. The choledoch diameter in men and women with FLD of the first age subgroup was greater relative to patients of the control group and of the preoperative period and also relative to older patients of the same group. In the long-term period after cholecystectomy in the group with fatty liver disease, the opportunistic microflora prevailed over essential one, and aerobic microflora over anaerobic one. Besides, increased amount of fungi and viruses was noted, as well as a change in the distribution of obligate microflora due to decrease in the content of eubacteria and predomination of lactobacteria over bifidobacteria in the parietal layer of the intestine. In the individuals of older age, reduction of the obligate and increase in the opportunistic microflora including aerobic one, was noted. \u0000CONCLUSIONS: Age, autonomic regulation and body mass have a significant influence on the formation of FLD in the long-term period after cholecystectomy. In the group of older patients, the influence of the autonomic regulation is lower, and increase in body mass is higher than in younger individuals.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129770759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniil O. Parot'kin, Natal'ya G. Bogdanova, G. Nazarova, S. Sudakov
INTRODUCTION: Use of sodium glutamate solution with food is a widely spread practice. The glutamate-ergic system has been shown to directly participate in the mechanisms of depression, however, up to the moment, no data have been found to evidence that use of sodium glutamate causes depression. AIM: To study the effect of intake of sodium glutamate on the formation of depression-like behavior in male rats. MATERIALS AND METHODS: Formation of depression-like behavior was evaluated in male rats of Wistar line with 230 g250 g weight at the beginning of the experiment in the situation of inescapable swimming according to the method of R. D. Porsolt, and of hanging by the tail according to T. A. Voronina. In the course of the experiment, the rats of the experimental group consumed 1.1% sodium glutamate solution daily for 30 days, the control animals drank water. During the experiment, the rats were kept in individual cages and had free access to water. The animals of the control group (n = 7) had only water in the drinking bowls. The animals of the experimental group (n = 7) were given water in one drinking bowl and 60 mМ (1.1%) sodium glutamate solution (Henan Lotus Flower Gourmet Powder Cо., LTD, China) in the other. RESULTS: Consumption of 1.1% sodium glutamate solution for 30 days led to reduction of the time of active movements and to increase in the number of periods of immobilization in animals in both tests. Besides, in the tests for depression-like behavior of animals, increased rhythmologic index of depression was found in the group of rats receiving sodium glutamate solution. CONCLUSION: Based on the results of study, it was found that chronic voluntary consumption of 60 mM (1.1%) sodium glutamate solution for 30 days provokes the formation of depression-like behavior in rats.
简介:在食品中使用谷氨酸钠溶液是一种广泛的做法。谷氨酸能系统已被证明直接参与抑郁症的机制，然而，到目前为止，还没有发现证据表明使用谷氨酸钠会导致抑郁症。目的:研究谷氨酸钠摄入对雄性大鼠抑郁样行为形成的影响。材料与方法:实验开始时，体重230 g250 g的Wistar系雄性大鼠在R. D. Porsolt法不可避免游泳和T. A. Voronina法吊尾的情况下，评估抑郁样行为的形成。在实验过程中，实验组大鼠每天饮用1.1%谷氨酸钠溶液，连续30 d，对照组大鼠饮水。在实验中，老鼠被关在单独的笼子里，可以自由饮水。对照组(n = 7)只在饮水碗中喝水。实验组动物(n = 7)饮水1碗，灌胃60 mМ(1.1%)谷氨酸钠溶液(河南莲花味精)。(中国)。结果:服用1.1%谷氨酸钠溶液30天，两组动物的活动时间均减少，固定时间均增加。此外，在动物抑郁样行为实验中，谷氨酸钠溶液组大鼠抑郁节律指标升高。结论:根据研究结果，长期自愿饮用60 mM(1.1%)谷氨酸钠溶液30天，可引起大鼠抑郁样行为的形成。
{"title":"Voluntary Consumption of Sodium Glutamate Solution as a Factor of Depression-Like Behavior in Adult Rats: an Experimental Study","authors":"Daniil O. Parot'kin, Natal'ya G. Bogdanova, G. Nazarova, S. Sudakov","doi":"10.17816/pavlovj109411","DOIUrl":"https://doi.org/10.17816/pavlovj109411","url":null,"abstract":"INTRODUCTION: Use of sodium glutamate solution with food is a widely spread practice. The glutamate-ergic system has been shown to directly participate in the mechanisms of depression, however, up to the moment, no data have been found to evidence that use of sodium glutamate causes depression. \u0000AIM: To study the effect of intake of sodium glutamate on the formation of depression-like behavior in male rats. \u0000MATERIALS AND METHODS: Formation of depression-like behavior was evaluated in male rats of Wistar line with 230 g250 g weight at the beginning of the experiment in the situation of inescapable swimming according to the method of R. D. Porsolt, and of hanging by the tail according to T. A. Voronina. In the course of the experiment, the rats of the experimental group consumed 1.1% sodium glutamate solution daily for 30 days, the control animals drank water. During the experiment, the rats were kept in individual cages and had free access to water. The animals of the control group (n = 7) had only water in the drinking bowls. The animals of the experimental group (n = 7) were given water in one drinking bowl and 60 mМ (1.1%) sodium glutamate solution (Henan Lotus Flower Gourmet Powder Cо., LTD, China) in the other. \u0000RESULTS: Consumption of 1.1% sodium glutamate solution for 30 days led to reduction of the time of active movements and to increase in the number of periods of immobilization in animals in both tests. Besides, in the tests for depression-like behavior of animals, increased rhythmologic index of depression was found in the group of rats receiving sodium glutamate solution. \u0000CONCLUSION: Based on the results of study, it was found that chronic voluntary consumption of 60 mM (1.1%) sodium glutamate solution for 30 days provokes the formation of depression-like behavior in rats.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132414532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. A. Mertsalov, E. P. Kulikov, V. V. Strel'nikov, Aleksey I. Kalinkin, Evgeniya I. Shumskaya, R. Piskunov
INTRODUCTION: Colorectal cancer (CRC) is a sure leader among malignant neoplasms (12.3%), without taking into account gender. Five-year survival rate in stage I CRC is 91%, in stage IV 14%. The currently existing treatment methods are helpless to significantly reduce mortality the approaches should be personalized and include the use of molecular genetic methods. AIM: To perform a comparative evaluation of expression profile of samples of tumor and healthy colon tissue in CRC. MATERIALS AND METHODS: The material for the study was 19 samples of tumor tissue taken from the pathologically altered colonic mucosa of 19 patients with CRC, and 7 samples of healthy tissue taken 10 cm12 cm distally or proximally from the visual boundary of the tumor. Biopsy materials were homogenized using a mechanical method. The quality and quantity of ribonucleic acid in the eluted solution were evaluated using IMPLEN nanospectrophotometer (Germany). Gene expression was evaluated using microchip kit SurePrint G3 HumanGeneExpv3 ArrayKit (Agilent, USА). Microchips were scanned on InnoScan 1100 AL apparatus (США) with subsequent image processing in Mapix Software program (USA). RESULTS: The analysis of expression profile demonstrated 505 differentially expressed genes, 337 of which showed reduced expression and 168 enhanced expression in the tumor material. The highest expression was demonstrated by genes bound with miRNA: hsa-miR-29b-3p and hsa-miR-1-5p, and also genes Н19, FOXQ1, INHBA, MMP1, CDH3, CXCL2, MDFI, THBS2. On the contrary, genes TMIGD1, GUCA2B, ZG16, AQP8, SLC4A4, CDKN2B-AS1, CA4, СА1 demonstrated a low expression in the tumor material. Expression of genes responsible for functioning of signal pathways: IL-17, NF-kappa B, TNF, was increased in tumor samples. Genes responsible for signal pathways Fatty acid degradation, Drug metabolism cytochrome P450, Metabolic pathways, Fatty acid metabolism and Steroid hormone biosynthesis, showed reduced expression. CONCLUSION: Significant differences were found in the expression profile of tumor and healthy tissue in patients with CRC. A comparative analysis of gene enrichment and the data of the international databases permitted to identify a number of terms, genes, clusters that can be used in future in search for predictors of prognosis and of response to treatment.
{"title":"Comparative Analysis of Gene Expression Profile in Tumor and Healthy Tissue in Patients with Colorectal Cancer","authors":"S. A. Mertsalov, E. P. Kulikov, V. V. Strel'nikov, Aleksey I. Kalinkin, Evgeniya I. Shumskaya, R. Piskunov","doi":"10.17816/pavlovj134974","DOIUrl":"https://doi.org/10.17816/pavlovj134974","url":null,"abstract":"INTRODUCTION: Colorectal cancer (CRC) is a sure leader among malignant neoplasms (12.3%), without taking into account gender. Five-year survival rate in stage I CRC is 91%, in stage IV 14%. The currently existing treatment methods are helpless to significantly reduce mortality the approaches should be personalized and include the use of molecular genetic methods. \u0000AIM: To perform a comparative evaluation of expression profile of samples of tumor and healthy colon tissue in CRC. \u0000MATERIALS AND METHODS: The material for the study was 19 samples of tumor tissue taken from the pathologically altered colonic mucosa of 19 patients with CRC, and 7 samples of healthy tissue taken 10 cm12 cm distally or proximally from the visual boundary of the tumor. Biopsy materials were homogenized using a mechanical method. The quality and quantity of ribonucleic acid in the eluted solution were evaluated using IMPLEN nanospectrophotometer (Germany). Gene expression was evaluated using microchip kit SurePrint G3 HumanGeneExpv3 ArrayKit (Agilent, USА). Microchips were scanned on InnoScan 1100 AL apparatus (США) with subsequent image processing in Mapix Software program (USA). \u0000RESULTS: The analysis of expression profile demonstrated 505 differentially expressed genes, 337 of which showed reduced expression and 168 enhanced expression in the tumor material. The highest expression was demonstrated by genes bound with miRNA: hsa-miR-29b-3p and hsa-miR-1-5p, and also genes Н19, FOXQ1, INHBA, MMP1, CDH3, CXCL2, MDFI, THBS2. On the contrary, genes TMIGD1, GUCA2B, ZG16, AQP8, SLC4A4, CDKN2B-AS1, CA4, СА1 demonstrated a low expression in the tumor material. Expression of genes responsible for functioning of signal pathways: IL-17, NF-kappa B, TNF, was increased in tumor samples. Genes responsible for signal pathways Fatty acid degradation, Drug metabolism cytochrome P450, Metabolic pathways, Fatty acid metabolism and Steroid hormone biosynthesis, showed reduced expression. \u0000CONCLUSION: Significant differences were found in the expression profile of tumor and healthy tissue in patients with CRC. A comparative analysis of gene enrichment and the data of the international databases permitted to identify a number of terms, genes, clusters that can be used in future in search for predictors of prognosis and of response to treatment.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130319120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. M. Uryasyev, A. V. Solov’yeva, Aleksey V. Cheskidov, Anastasiya A. Filimonova, E. А. Nikiforova
INTRODUCTION: Obesity is one of the leading risk factors for the development of cardiovascular diseases. At present, of the greatest scientific interest are local deposits of adipose tissue as a possible morphological substrate for the development of this group of diseases. Along with the traditional risk factors, one more risk factor for a poor prognosis of cardiovascular disease can be a change in the thickness of the epicardial fat (EF) and of the interatrial septum (IAS) in case of its lipomatosis. AIM: To study the significance of EF and of IAS thickness as markers of cardiovascular risk in coronary heart disease. MATERIALS AND METHODS: The study involved 116 individuals with coronary heart disease (55 men (47%) and 61 women (53%), mean age 68 [61; 72] years). Thickness of EF and IAS was evaluated by the method of echocardiography. The frequency of poor outcomes was evaluated using KaplanMeier method, ROC-curve analysis. The differences were considered statistically significant at p 0.05. RESULTS: In the group of patients with unstable angina, the threshold value of IAS thickness was 0.7 cm (p 0.001), and of EF thickness 0.8 cm (2 = 10.89, p = 0.001), in the group of patients with myocardial infarction 0.7 cm (p 0.001) and 0.8 cm (p 0.001), respectively. In the group of patient with unstable angina, IAS thickness 0.7 cm (2 = 10.3, p = 0.0013) and EF thickness 0.8 cm (2 = 10.89, p = 0.001) demonstrate a poor prognosis in comparison with the parameters below the threshold values. In the group of patients with myocardial infarction, IAS thickness 0.7 cm (2 = 8.4, p = 0.005) and EF 0.8 cm (2 = 9.66, p = 0.0019) demonstrate poor prognosis in comparison with the parameters below the threshold values. CONCLUSION: In unstable angina and myocardial infarction, the value of IAS thickness 0.7 cm (p = 0.0013 and p = 0.005, respectively) and of EF thickness 0.8 cm (p = 0.001 and p = 0.0019, respectively) present a poor prognosis compared to lower values. It is recommended that EF and IAS thickness be determined in echocardiography as an additional marker for a poor prognosis in coronary heart disease.
简介:肥胖是心血管疾病发展的主要危险因素之一。目前，最大的科学兴趣是脂肪组织的局部沉积作为这组疾病发展的可能形态学基础。除了传统的危险因素外，心血管疾病预后不良的另一个危险因素可能是心外膜脂肪(EF)和房间隔(IAS)脂肪化时厚度的变化。目的:探讨EF和IAS厚度作为冠心病心血管危险指标的意义。材料和方法:研究纳入116例冠心病患者，其中男性55例(47%)，女性61例(53%)，平均年龄68岁[61;72年)。超声心动图法评价EF和IAS厚度。采用KaplanMeier法、roc曲线分析评估不良预后的频率。p < 0.05认为差异有统计学意义。结果:不稳定心绞痛组IAS厚度阈值为0.7 cm (p 0.001)， EF厚度阈值为0.8 cm (2 = 10.89, p = 0.001)，心肌梗死组IAS厚度阈值为0.7 cm (p 0.001)， EF厚度阈值为0.8 cm (p 0.001)。在不稳定型心绞痛患者组中，IAS厚度0.7 cm (2 = 10.3, p = 0.0013)和EF厚度0.8 cm (2 = 10.89, p = 0.001)与低于阈值的参数相比，预后较差。在心肌梗死患者组中，IAS厚度0.7 cm (2 = 8.4, p = 0.005)和EF 0.8 cm (2 = 9.66, p = 0.0019)与低于阈值的参数相比，预后较差。结论:在不稳定型心绞痛和心肌梗死中，IAS厚度为0.7 cm (p = 0.0013和p = 0.005)和EF厚度为0.8 cm (p = 0.001和p = 0.0019)的患者预后较差。建议在超声心动图中确定EF和IAS厚度，作为冠心病预后不良的额外标志。
{"title":"Prognostic Significance of Cardiac Fat Deposits in Patients with Coronary Heart Disease","authors":"O. M. Uryasyev, A. V. Solov’yeva, Aleksey V. Cheskidov, Anastasiya A. Filimonova, E. А. Nikiforova","doi":"10.17816/pavlovj322796","DOIUrl":"https://doi.org/10.17816/pavlovj322796","url":null,"abstract":"INTRODUCTION: Obesity is one of the leading risk factors for the development of cardiovascular diseases. At present, of the greatest scientific interest are local deposits of adipose tissue as a possible morphological substrate for the development of this group of diseases. Along with the traditional risk factors, one more risk factor for a poor prognosis of cardiovascular disease can be a change in the thickness of the epicardial fat (EF) and of the interatrial septum (IAS) in case of its lipomatosis. \u0000AIM: To study the significance of EF and of IAS thickness as markers of cardiovascular risk in coronary heart disease. \u0000MATERIALS AND METHODS: The study involved 116 individuals with coronary heart disease (55 men (47%) and 61 women (53%), mean age 68 [61; 72] years). Thickness of EF and IAS was evaluated by the method of echocardiography. The frequency of poor outcomes was evaluated using KaplanMeier method, ROC-curve analysis. The differences were considered statistically significant at p 0.05. \u0000RESULTS: In the group of patients with unstable angina, the threshold value of IAS thickness was 0.7 cm (p 0.001), and of EF thickness 0.8 cm (2 = 10.89, p = 0.001), in the group of patients with myocardial infarction 0.7 cm (p 0.001) and 0.8 cm (p 0.001), respectively. In the group of patient with unstable angina, IAS thickness 0.7 cm (2 = 10.3, p = 0.0013) and EF thickness 0.8 cm (2 = 10.89, p = 0.001) demonstrate a poor prognosis in comparison with the parameters below the threshold values. In the group of patients with myocardial infarction, IAS thickness 0.7 cm (2 = 8.4, p = 0.005) and EF 0.8 cm (2 = 9.66, p = 0.0019) demonstrate poor prognosis in comparison with the parameters below the threshold values. \u0000CONCLUSION: In unstable angina and myocardial infarction, the value of IAS thickness 0.7 cm (p = 0.0013 and p = 0.005, respectively) and of EF thickness 0.8 cm (p = 0.001 and p = 0.0019, respectively) present a poor prognosis compared to lower values. It is recommended that EF and IAS thickness be determined in echocardiography as an additional marker for a poor prognosis in coronary heart disease.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124088465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Belova, R. Zubavlenko, E. V. Gladkova, I. Babushkina, V. Ul'yanov
INTRODUCTION: Posttraumatic osteoarthrosis (PTOA) resulting from injuries of connective-tissue components of the joint, is accompanied by formation of free radicals activating chondro- and osteoresorption, which leads to fragmentation of biopolymers of the extracellular matrix of the joint tissues. AIM: To study the peculiarities of free radical oxidation and metabolic processes of cartilage and bone tissues in animals with a surgical model of PTOA of the knee joint. MATERIALS AND METHODS: The study was conducted on 31 rats (11 intact animals and 20 animals with PTOA model). The metabolic processes of the connective tissue were evaluated by the changes in the content of biomarkers of the cartilage (hyaluronan, aggrecan) and bone (fibroblast growth factor-23, osteprotegerin, sclerostin, osteocalcin) tissues. The condition of the free radical oxidation processes was evaluated by the level of lipid hydroperoxides, and the activity of antioxidant system by the parameters of the total antioxidant and thiol statuses. RESULTS: In rats with the PTOA model, an increase in cartilage tissue biopolymers aggrecan and hyaluronan (p 0.001) was noted with a negative change in the marker of bone formation (a tendency to increase in the content of osteocalcin) and markers of regulation of bone homeostasis (increased fibroblast growth factor-23, p 0.001), with a tendency to decrease in the content of osteoprotegerin and sclerostin, in comparison with intact animals of the control group. In parallel with this, an increase in lipid hydroperoxides (p 0.01) in the systemic bloodstream was detected with a decrease in the thiol status index (p 0.01) with preserved normal total antioxidant activity (p 0.05). CONCLUSION: The data of the conducted study evidences intensification of free radical oxidation and derangement of metabolic processes in the bone and cartilage tissues in animals with a surgical model of PTOA of the knee joint. A negative change in the metabolism of the bone tissue was manifested by the loss of balance of remodeling processes, and metabolic disorders in the cartilage tissue consisted in the destruction of its biopolymers in conditions of intensification of free radical oxidation processes and relative tension of the thiol system with the total antioxidant activity remaining within the physiological norm. The established facts are promising from the point of view of using the studied biomarkers both for the identification of pathogenetic triggers of PTOA of the knee joint, and for the determination of the direction of therapeutic measures.
{"title":"Free Radical Oxidation and Metabolic Processes of Cartilage and Bone Tissues in Animals with Surgical Model of Posttraumatic Osteoarthrosis","authors":"S. Belova, R. Zubavlenko, E. V. Gladkova, I. Babushkina, V. Ul'yanov","doi":"10.17816/pavlovj111575","DOIUrl":"https://doi.org/10.17816/pavlovj111575","url":null,"abstract":"INTRODUCTION: Posttraumatic osteoarthrosis (PTOA) resulting from injuries of connective-tissue components of the joint, is accompanied by formation of free radicals activating chondro- and osteoresorption, which leads to fragmentation of biopolymers of the extracellular matrix of the joint tissues. \u0000AIM: To study the peculiarities of free radical oxidation and metabolic processes of cartilage and bone tissues in animals with a surgical model of PTOA of the knee joint. \u0000MATERIALS AND METHODS: The study was conducted on 31 rats (11 intact animals and 20 animals with PTOA model). The metabolic processes of the connective tissue were evaluated by the changes in the content of biomarkers of the cartilage (hyaluronan, aggrecan) and bone (fibroblast growth factor-23, osteprotegerin, sclerostin, osteocalcin) tissues. The condition of the free radical oxidation processes was evaluated by the level of lipid hydroperoxides, and the activity of antioxidant system by the parameters of the total antioxidant and thiol statuses. \u0000RESULTS: In rats with the PTOA model, an increase in cartilage tissue biopolymers aggrecan and hyaluronan (p 0.001) was noted with a negative change in the marker of bone formation (a tendency to increase in the content of osteocalcin) and markers of regulation of bone homeostasis (increased fibroblast growth factor-23, p 0.001), with a tendency to decrease in the content of osteoprotegerin and sclerostin, in comparison with intact animals of the control group. In parallel with this, an increase in lipid hydroperoxides (p 0.01) in the systemic bloodstream was detected with a decrease in the thiol status index (p 0.01) with preserved normal total antioxidant activity (p 0.05). \u0000CONCLUSION: The data of the conducted study evidences intensification of free radical oxidation and derangement of metabolic processes in the bone and cartilage tissues in animals with a surgical model of PTOA of the knee joint. A negative change in the metabolism of the bone tissue was manifested by the loss of balance of remodeling processes, and metabolic disorders in the cartilage tissue consisted in the destruction of its biopolymers in conditions of intensification of free radical oxidation processes and relative tension of the thiol system with the total antioxidant activity remaining within the physiological norm. The established facts are promising from the point of view of using the studied biomarkers both for the identification of pathogenetic triggers of PTOA of the knee joint, and for the determination of the direction of therapeutic measures.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"216 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133993166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Fedoseyev, Aleksandr A. Chekushin, R. V. Tishkin, Aleksey N. Dobychin, Grigoriy Yu. Zenkin, Aleksandra D. Prints, Mariya S. Melyokhina, Anna V. Tkachik, Andrey A. Zubov
INTRODUCTION: Osteoarthritis (OA) of the knee joint (KJ) is a common problem. The basic factors impairing the function of the KJ are pain and restriction of movement. Evaluation of function of the KJ in OA is an indispensable part of the diagnostic and treatment process. There exists a problem of a quantitative evaluation of pain and restriction of movement of the KJ in OA, associated with the absence of a single standard for evaluation of the KJ function. In the article, the data are given on the modern possibilities for evaluation of the KJ function in OA. The KJ function is evaluated in clinical examination, functional tests, numerous functional questionnaires, as well as with the use of devices for record of movements. Clinical picture of OA of the KJ is evaluated in the clinical examination which includes diagnostic tests, evaluation of the volume of movement, force of muscles, with conclusions about the phenotype of the disease. Functional tests recommended by OARSI (Osteoarthritis Research Society International) are preferable for express-testing, but not all of them have reference values. Functional questionnaires for evaluation of the KJ function exist in many variants, which impedes their choice and interaction between specialists, and they are characterized by different labor intensity in use. There exists a diversity of devices for evaluation of function of the KJ, some of which are expensive, require special rooms, training of the specialist, and take considerable time. Others are simpler in use and inexpensive, but give less reliable results. CONCLUSION: The authors showed the possibilities of a comprehensive evaluation, described the problem of the lack of a single standard for investigation of the KJ function. A conclusion is made about the necessity to develop a software-hardware complex that maximally satisfies the criteria of objectivity, accessibility and quickness of use for evaluation of function of the KJ in OA.
{"title":"Complex Approach in Examination of Function of the Knee Joint in Patients with Osteoarthritis","authors":"A. Fedoseyev, Aleksandr A. Chekushin, R. V. Tishkin, Aleksey N. Dobychin, Grigoriy Yu. Zenkin, Aleksandra D. Prints, Mariya S. Melyokhina, Anna V. Tkachik, Andrey A. Zubov","doi":"10.17816/pavlovj109633","DOIUrl":"https://doi.org/10.17816/pavlovj109633","url":null,"abstract":"INTRODUCTION: Osteoarthritis (OA) of the knee joint (KJ) is a common problem. The basic factors impairing the function of the KJ are pain and restriction of movement. Evaluation of function of the KJ in OA is an indispensable part of the diagnostic and treatment process. There exists a problem of a quantitative evaluation of pain and restriction of movement of the KJ in OA, associated with the absence of a single standard for evaluation of the KJ function. In the article, the data are given on the modern possibilities for evaluation of the KJ function in OA. The KJ function is evaluated in clinical examination, functional tests, numerous functional questionnaires, as well as with the use of devices for record of movements. Clinical picture of OA of the KJ is evaluated in the clinical examination which includes diagnostic tests, evaluation of the volume of movement, force of muscles, with conclusions about the phenotype of the disease. Functional tests recommended by OARSI (Osteoarthritis Research Society International) are preferable for express-testing, but not all of them have reference values. Functional questionnaires for evaluation of the KJ function exist in many variants, which impedes their choice and interaction between specialists, and they are characterized by different labor intensity in use. There exists a diversity of devices for evaluation of function of the KJ, some of which are expensive, require special rooms, training of the specialist, and take considerable time. Others are simpler in use and inexpensive, but give less reliable results. \u0000CONCLUSION: The authors showed the possibilities of a comprehensive evaluation, described the problem of the lack of a single standard for investigation of the KJ function. A conclusion is made about the necessity to develop a software-hardware complex that maximally satisfies the criteria of objectivity, accessibility and quickness of use for evaluation of function of the KJ in OA.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115488686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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