T. Ooie, Masato Tanaka, Yuji Yamachoshi, Tomonori Nakahara, K. Abe, M. Kataoka
ELISA(Enzyme-Linked ImmunoSorbent Assay)法は極微量 の抗体、あるいは抗原の濃度を検出するのに用いられる。 特異性の高い抗原抗体反応を利用し、酵素反応に基づく発 色・発光等を測定することで、多種のタンパク質が混在す る生体試料中から特定のタンパク質を検出・定量すること ができるが、マイクロプレートを用いる血液検査では、1 項目あたり 20~ 100 lのサンプルが必要で、測定に数時 間を要する。医療現場や在宅でのその場診断、すなわち POCT (Point Of Care Testing)用途では、必要サンプル量の 低減と測定時間の短縮が不可欠である。我々は、インジェ クタを用いて、プラスチック基板上のマイクロ流路底部に 抗体溶液を吐出・固定化する独自の技術を用いて、マイク ロ流路内でサンドイッチ ELISAを実現し、極微量サンプル での抗原検出に成功した 。これは、大型の分析装置を小 型化しようとするMicro Total Analysis System (micro-TAS) の流れとは異なったアプローチであり、低コスト化が容易 な点が特徴である。本手法における、抗体固定化面の表 面処理としては、市販のマイクロプレートと同じポリマー コーティングを用いているが、ウェットプロセスであるこ とから、(1)溝底面エッジ部の処理が不均一となりやすい、(2) 処理に時間を要する等の課題がある。本研究では、UVレー ザ照射による表面処理で、これらの課題を解決することを 目的とする。 2.実験方法
ELISA(Enzyme-Linked ImmunoSorbent Assay)法用于检测极微量抗体或抗原的浓度。利用特异性高的抗原和抗体反应，测定基于酶反应的发色、发光等，从混合有多种蛋白质的生物样品中检测并定量特定的蛋白质。,可以使用微板块血液检查每一个项目中,20 ~ 100l需要的样品,测定需要数时间。在医疗现场和居家的当场诊断，即POCT (Point Of Care Testing)应用中，降低所需样本量和缩短测量时间是不可或缺的。我们采用独特的技术，在塑料底板上的微流道底部喷射并固化抗体溶液，在微流道内实现了夹层ELISA，获得了极微量样本中抗原检测成功了。这与Micro Total Analysis System (Micro - tas)的趋势不同，Micro - tas是将大型分析设备小型化的一种方法，其特点是容易降低成本。在本方法中，作为抗体固定面的表面处理，使用了与市售微板相同的聚合物涂层，但是是湿工艺。另外，存在(1)槽底面边缘部的处理容易不均匀、(2)处理需要时间等问题。本研究的目的是通过利用UV激光照射的表面处理来解决这些问题。2.实验方法
{"title":"Laser Surface Treatment of Plastics for a Single-Channel Multiple Immunoassays Chip","authors":"T. Ooie, Masato Tanaka, Yuji Yamachoshi, Tomonori Nakahara, K. Abe, M. Kataoka","doi":"10.7791/JHTS.37.128","DOIUrl":"https://doi.org/10.7791/JHTS.37.128","url":null,"abstract":"ELISA(Enzyme-Linked ImmunoSorbent Assay)法は極微量 の抗体、あるいは抗原の濃度を検出するのに用いられる。 特異性の高い抗原抗体反応を利用し、酵素反応に基づく発 色・発光等を測定することで、多種のタンパク質が混在す る生体試料中から特定のタンパク質を検出・定量すること ができるが、マイクロプレートを用いる血液検査では、1 項目あたり 20~ 100 lのサンプルが必要で、測定に数時 間を要する。医療現場や在宅でのその場診断、すなわち POCT (Point Of Care Testing)用途では、必要サンプル量の 低減と測定時間の短縮が不可欠である。我々は、インジェ クタを用いて、プラスチック基板上のマイクロ流路底部に 抗体溶液を吐出・固定化する独自の技術を用いて、マイク ロ流路内でサンドイッチ ELISAを実現し、極微量サンプル での抗原検出に成功した 。これは、大型の分析装置を小 型化しようとするMicro Total Analysis System (micro-TAS) の流れとは異なったアプローチであり、低コスト化が容易 な点が特徴である。本手法における、抗体固定化面の表 面処理としては、市販のマイクロプレートと同じポリマー コーティングを用いているが、ウェットプロセスであるこ とから、(1)溝底面エッジ部の処理が不均一となりやすい、(2) 処理に時間を要する等の課題がある。本研究では、UVレー ザ照射による表面処理で、これらの課題を解決することを 目的とする。 2.実験方法","PeriodicalId":113412,"journal":{"name":"Journal of High Temperature Society","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127262343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effct of Bump Condition on Flip-Chip Bondability","authors":"T. Tomioka, Tomohiro Iguchi, Koichiro Atsumi","doi":"10.7791/JHTS.30.41","DOIUrl":"https://doi.org/10.7791/JHTS.30.41","url":null,"abstract":"","PeriodicalId":113412,"journal":{"name":"Journal of High Temperature Society","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129106420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Kawabata, Keita Kasamoto, Hideki Nakazato, T. Usui
Experiments to inhibit the formation of hazardous substances such as benzenes and dioxins in a combustion furnace were carried out by the centrifugation and recombustion method of fly unburned matters (fly carbonaceous matters (C*)) in an exhaust gas at high temperatures. Total dioxin’s and Cl5 benzene’s concentrations in the exhaust gas have a good correlation with the number of the fly C* remaining in the exhaust gas, and their concentrations increase as the number of the fly C* rises. Total dioxin’s and Cl5 benzene’s concentrations after the centrifugation and recombustion with the present cyclone reduce to 10% and 20% of their initial concentrations in the exhaust gas from combustion furnace, respectively, in proportion to the decrease in the fly C*. On the other hand, in the case of low dioxin’s and benzene’s concentrations in the exhaust gas under the complete combustion conditions their reduction ratios become worse. The reason why their reduction ratios decrease is considered a decrease in the partial separation efficiency for fine particles in the present cyclone, which is attributed to the increase in the relative ratio of the fly C* with the sizes smaller than 2μm under the complete combustion conditions. The predictive equation of the partial separation efficiency at high temperatures obtained for the present cyclone of a small size indicates that the reduction ratios of dioxins and benzenes are much improved by using the cyclone of larger sizes and increasing in the inlet velocity of the exhaust gas to the cyclone. The present centrifugation and recombustion method of the exhaust gas at high temperatures is expected to be useful for the actual incineration plants.
{"title":"Inhibition of Hazardous Substances Formation in a Combustion Furnace by Centrifugation and Recombustion Method of Fly Unburned Matters at High Temperatures","authors":"H. Kawabata, Keita Kasamoto, Hideki Nakazato, T. Usui","doi":"10.7791/JHTS.33.200","DOIUrl":"https://doi.org/10.7791/JHTS.33.200","url":null,"abstract":"Experiments to inhibit the formation of hazardous substances such as benzenes and dioxins in a combustion furnace were carried out by the centrifugation and recombustion method of fly unburned matters (fly carbonaceous matters (C*)) in an exhaust gas at high temperatures. Total dioxin’s and Cl5 benzene’s concentrations in the exhaust gas have a good correlation with the number of the fly C* remaining in the exhaust gas, and their concentrations increase as the number of the fly C* rises. Total dioxin’s and Cl5 benzene’s concentrations after the centrifugation and recombustion with the present cyclone reduce to 10% and 20% of their initial concentrations in the exhaust gas from combustion furnace, respectively, in proportion to the decrease in the fly C*. On the other hand, in the case of low dioxin’s and benzene’s concentrations in the exhaust gas under the complete combustion conditions their reduction ratios become worse. The reason why their reduction ratios decrease is considered a decrease in the partial separation efficiency for fine particles in the present cyclone, which is attributed to the increase in the relative ratio of the fly C* with the sizes smaller than 2μm under the complete combustion conditions. The predictive equation of the partial separation efficiency at high temperatures obtained for the present cyclone of a small size indicates that the reduction ratios of dioxins and benzenes are much improved by using the cyclone of larger sizes and increasing in the inlet velocity of the exhaust gas to the cyclone. The present centrifugation and recombustion method of the exhaust gas at high temperatures is expected to be useful for the actual incineration plants.","PeriodicalId":113412,"journal":{"name":"Journal of High Temperature Society","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131057185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Tani, S. Uematsu, M. Yoshioka, T. Takabatake, Y. Namba, Y. Ishihara, Takahiro Mishima, Y. Kawanami
Anti-cavitation erosion performances of thermally sprayed cobalt-based alloy coatings were compared with that of aluminum bronze castings, which have been utilized as s substrate for propeller in marine vessel because of its formability, endurance against seawater, and the cavitation erosion resistance. Cobalt-based alloy coatings are fabricated by using three thermal spray processes: atmospheric plasma spray, low-pressure plasma spray and high velocity oxygen fuel flame spray. The ultrasonic vibration type cavitation erosion testing apparatus were employed for the cavitation erosion rate measurement. The cavitation erosion performances determined by the specific volume loss rate of low-pressure plasma-sprayed cobalt-based alloy and HVOF sprayed cobalt-based alloy coatings were improved five times and twice compared with that of aluminum bronze castings, respectively. The anti-cavitation erosion performance of atmospheric plasma sprayed cobalt-based alloy coating did not reach the level of aluminum bronze castings.
{"title":"Development of Advanced Anti-cavitation Erosion Performances in Thermally-sprayed Co-based Alloy Coatings","authors":"K. Tani, S. Uematsu, M. Yoshioka, T. Takabatake, Y. Namba, Y. Ishihara, Takahiro Mishima, Y. Kawanami","doi":"10.7791/jhts.35.301","DOIUrl":"https://doi.org/10.7791/jhts.35.301","url":null,"abstract":"Anti-cavitation erosion performances of thermally sprayed cobalt-based alloy coatings were compared with that of aluminum bronze castings, which have been utilized as s substrate for propeller in marine vessel because of its formability, endurance against seawater, and the cavitation erosion resistance. Cobalt-based alloy coatings are fabricated by using three thermal spray processes: atmospheric plasma spray, low-pressure plasma spray and high velocity oxygen fuel flame spray. The ultrasonic vibration type cavitation erosion testing apparatus were employed for the cavitation erosion rate measurement. The cavitation erosion performances determined by the specific volume loss rate of low-pressure plasma-sprayed cobalt-based alloy and HVOF sprayed cobalt-based alloy coatings were improved five times and twice compared with that of aluminum bronze castings, respectively. The anti-cavitation erosion performance of atmospheric plasma sprayed cobalt-based alloy coating did not reach the level of aluminum bronze castings.","PeriodicalId":113412,"journal":{"name":"Journal of High Temperature Society","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126996935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Numerical Analysis of Oxygen Induction Plasmas with Higher-Order Approximation of Transport Properties","authors":"Takayuki Watanabe, N. Sugimoto, N. Atsuchi","doi":"10.7791/JHTS.30.122","DOIUrl":"https://doi.org/10.7791/JHTS.30.122","url":null,"abstract":"","PeriodicalId":113412,"journal":{"name":"Journal of High Temperature Society","volume":"256 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130784034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
*新日本製鐵(株) 環境・プロセス研究開発センター(〒 293-8511 千葉県富津市新富 20-1) Nippon Steel Corporation Environment & Process Technology Center (20-1,Shintomi, Futtsu, Chiba, 293-8511, JAPAN) **新日本製鐵(株) 技術総括部エネルギー技術 Gr(〒 100-8071 東京都千代田区大手町 2-6-3) Nippon Steel Corporation, Technical Administration & Planning Department, Energy Technical Group (2-6-3, Otemachi , Chiyoda-ku, Tokyo, 100-8071, JAPAN ) ***新日本製鐵(株) 技術総括部技術総括 Gr(〒 100-8071 東京都千代田区大手町 2-6-3 ) Nippon Steel Corporation, Technical Administration & Planning Department, Technical Administration & Planning Group (2-6-3, Otemachi , Chiyoda-ku, Tokyo, 100-8071, JAPAN ) 1.緒 言
*新日本制铁(株) 环境・プロセス研究开発センター(〒 293-8511 千叶県富津市新富 20-1) Nippon Steel Corporation Environment & Process Technology Center (20-1,Shintomi, Futtsu, Chiba, 293-8511, JAPAN) **新日本制铁(株) 技术総括部エネルギー技术 Gr(〒 100-8071 东京都千代田区大手町 2-6-3) Nippon Steel Corporation, Technical Administration & Planning Department, Energy Technical Group (2-6-3, Otemachi , Chiyoda-ku, Tokyo, 100-8071, JAPAN ) ***新日本制铁(株) 技术総括部技术総括 Gr(〒 100-8071 东京都千代田区大手町 2-6-3 ) Nippon Steel Corporation, Technical Administration & Planning Department, Technical Administration & Planning Group (2-6-3, Otemachi , Chiyoda-ku, Tokyo, 100-8071, JAPAN ) 1.绪 言
{"title":"日本鉄鋼業における省エネルギ-の現状と今後;日本鉄鋼業における省エネルギ-の現状と今後;Present status and future prospects of energy conservation in Japanese steel industry","authors":"M. Ichida, Tooru Ono, K. Yonezawa","doi":"10.7791/JHTS.34.96","DOIUrl":"https://doi.org/10.7791/JHTS.34.96","url":null,"abstract":"*新日本製鐵(株) 環境・プロセス研究開発センター(〒 293-8511 千葉県富津市新富 20-1) Nippon Steel Corporation Environment & Process Technology Center (20-1,Shintomi, Futtsu, Chiba, 293-8511, JAPAN) **新日本製鐵(株) 技術総括部エネルギー技術 Gr(〒 100-8071 東京都千代田区大手町 2-6-3) Nippon Steel Corporation, Technical Administration & Planning Department, Energy Technical Group (2-6-3, Otemachi , Chiyoda-ku, Tokyo, 100-8071, JAPAN ) ***新日本製鐵(株) 技術総括部技術総括 Gr(〒 100-8071 東京都千代田区大手町 2-6-3 ) Nippon Steel Corporation, Technical Administration & Planning Department, Technical Administration & Planning Group (2-6-3, Otemachi , Chiyoda-ku, Tokyo, 100-8071, JAPAN ) 1.緒 言","PeriodicalId":113412,"journal":{"name":"Journal of High Temperature Society","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127631755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seiichi Nagatome, Kouki Taguchi, S. Hyun, H. Nakajima
{"title":"Applications of Lotus-Type Porous Metal and Alloys for Aero Engine Components","authors":"Seiichi Nagatome, Kouki Taguchi, S. Hyun, H. Nakajima","doi":"10.7791/JHTS.34.66","DOIUrl":"https://doi.org/10.7791/JHTS.34.66","url":null,"abstract":"","PeriodicalId":113412,"journal":{"name":"Journal of High Temperature Society","volume":"140 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131352874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kenji Ishimura, T. Ida, M. Fuchihata, T. Honjo, H. Sano
Biomass pellet utilities are popular in North European as a pellet stove and boiler et al. But, we have a lot of problem on wood biomass utilities in social situations and geography conditions. Especially, to move of biomass from mountain area to user side transportation coast rises. Therefore, we have to improve for thermal energy characteristics in biomass based on moving. This technology is new carbonized technology for improvement of biomass thermal energy characteristics. This technology controls heated temperature and pressed force by hot-press method. Fundamental properties of biomass show thermal decomposition and ultimate analysis. In these results, we suggest to occur a half carbonized phenomena for improvement of thermal energy. Half carbonized phenomena begin approximately 540K in sawdust and 580K in cellulose. And, total calorifi c value suddenly increases in these heated temperatures. Sawdust could suggest occurring lower 40K to compare cellulose.
{"title":"Improvement for Thermal Energy Characteristics of Wood Biomass Pelletized Using a Half Carbonized Technology","authors":"Kenji Ishimura, T. Ida, M. Fuchihata, T. Honjo, H. Sano","doi":"10.7791/JHTS.33.38","DOIUrl":"https://doi.org/10.7791/JHTS.33.38","url":null,"abstract":"Biomass pellet utilities are popular in North European as a pellet stove and boiler et al. But, we have a lot of problem on wood biomass utilities in social situations and geography conditions. Especially, to move of biomass from mountain area to user side transportation coast rises. Therefore, we have to improve for thermal energy characteristics in biomass based on moving. This technology is new carbonized technology for improvement of biomass thermal energy characteristics. This technology controls heated temperature and pressed force by hot-press method. Fundamental properties of biomass show thermal decomposition and ultimate analysis. In these results, we suggest to occur a half carbonized phenomena for improvement of thermal energy. Half carbonized phenomena begin approximately 540K in sawdust and 580K in cellulose. And, total calorifi c value suddenly increases in these heated temperatures. Sawdust could suggest occurring lower 40K to compare cellulose.","PeriodicalId":113412,"journal":{"name":"Journal of High Temperature Society","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125430679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Dynamic Recrystallization on Diffusion Bonding of Ni3Al Intermetallic Compound","authors":"Yuichiro Koizumi, Y. Minamino, Y. Inui","doi":"10.7791/JHTS.30.204","DOIUrl":"https://doi.org/10.7791/JHTS.30.204","url":null,"abstract":"","PeriodicalId":113412,"journal":{"name":"Journal of High Temperature Society","volume":"344 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123103024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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