Pub Date : 2021-07-01DOI: 10.21608/djs.2021.206612
M. M. El-Sheekha, Mohammed Y. Bedaiwy, Aya A. El-ngar, EmanW Elgammal
Cellulolytic fungi, Cellulolytic activity, Congo red, Clear zone, Screening Cellulases are a series of hydrolytic enzymes that can hydrolyze cellulosic biomass into a simpler sugar. In the present study, thirty-one fungal species were isolated from different places in Al-Gharbia and Aswan governorates. These fungal species were identified and found to belong to twelve genera "Trichoderma, Cladosporium, Aspergillus, Botritrichom, Rhizopus, Cephalosporium, Fusarium, Penicillium, Mucor, Circinella, Alternaria and Rhodotorula". All identified fungal species were screened for cellulolytic activity based on clear zone diameter using 0.1% congo red. Out of thirty-one fungal species, 27 species showed cellulolytic activity and the highest activity was recorded by Trichoderma viride and isolate No. 1 of Aspergillus niger. All 27 fungal species were tested for three cellulolytic enzymes as "carboxymethyl cellulase (CMCase), βglucosidase (βGase), and filterpaper cellulase (FPase)". The highest total cellulolytic activity for three cellulolytic enzymes was recorded by isolate No. 1 of Aspergillus niger (79.3 U/ml). Among all tested isolates of Aspergillus niger isolate No. 1 isolated from Tanta was the best one.
{"title":"Isolation, identification and screening of cellulolytic activity of some fungi from different sources and localities in Egypt","authors":"M. M. El-Sheekha, Mohammed Y. Bedaiwy, Aya A. El-ngar, EmanW Elgammal","doi":"10.21608/djs.2021.206612","DOIUrl":"https://doi.org/10.21608/djs.2021.206612","url":null,"abstract":"Cellulolytic fungi, Cellulolytic activity, Congo red, Clear zone, Screening Cellulases are a series of hydrolytic enzymes that can hydrolyze cellulosic biomass into a simpler sugar. In the present study, thirty-one fungal species were isolated from different places in Al-Gharbia and Aswan governorates. These fungal species were identified and found to belong to twelve genera \"Trichoderma, Cladosporium, Aspergillus, Botritrichom, Rhizopus, Cephalosporium, Fusarium, Penicillium, Mucor, Circinella, Alternaria and Rhodotorula\". All identified fungal species were screened for cellulolytic activity based on clear zone diameter using 0.1% congo red. Out of thirty-one fungal species, 27 species showed cellulolytic activity and the highest activity was recorded by Trichoderma viride and isolate No. 1 of Aspergillus niger. All 27 fungal species were tested for three cellulolytic enzymes as \"carboxymethyl cellulase (CMCase), βglucosidase (βGase), and filterpaper cellulase (FPase)\". The highest total cellulolytic activity for three cellulolytic enzymes was recorded by isolate No. 1 of Aspergillus niger (79.3 U/ml). Among all tested isolates of Aspergillus niger isolate No. 1 isolated from Tanta was the best one.","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"293 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78711222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-01DOI: 10.21608/djs.2021.211321
R. Ghazy, F. Shehata, F. El-Mekawey, N. Sayed
{"title":"A Home –Made of Static Laser Scattering Experiment for Dopamine Investigations","authors":"R. Ghazy, F. Shehata, F. El-Mekawey, N. Sayed","doi":"10.21608/djs.2021.211321","DOIUrl":"https://doi.org/10.21608/djs.2021.211321","url":null,"abstract":"","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90458439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-01DOI: 10.21608/djs.2021.194670
E. Kenawy, Abdelbaset Shoker, Zeinab S. Ghaly, M. A. El-Meligy
Wound dressings are highly desired for the treatment of chronic wound infections. This study aimed to prepare membranes composed of chemically crosslinkedPVA and Gelatin polymers. Curcumin was added to improve the membrane's biological properties. FTIR spectroscopy was used to investigate their chemical structures, while SEM and TGA analysis were applied to studies the morphology of the membrane and the thermal properties of the crosslinked membranes. In addition, the contact angle measurement was also performed to get more information about the physicochemical properties. In addition, the biodegradability studies were performed and the findings highlighted that the prepared biodegradable PVA/ Gelatin/ Curcumin membranes could be efficiently used as dressers for enhancing wound healing.
{"title":"Chemically Crosslinked Polyvinyl Alcohol/ Gelatin Wound Dressing Loaded With Curcumin","authors":"E. Kenawy, Abdelbaset Shoker, Zeinab S. Ghaly, M. A. El-Meligy","doi":"10.21608/djs.2021.194670","DOIUrl":"https://doi.org/10.21608/djs.2021.194670","url":null,"abstract":"Wound dressings are highly desired for the treatment of chronic wound infections. This study aimed to prepare membranes composed of chemically crosslinkedPVA and Gelatin polymers. Curcumin was added to improve the membrane's biological properties. FTIR spectroscopy was used to investigate their chemical structures, while SEM and TGA analysis were applied to studies the morphology of the membrane and the thermal properties of the crosslinked membranes. In addition, the contact angle measurement was also performed to get more information about the physicochemical properties. In addition, the biodegradability studies were performed and the findings highlighted that the prepared biodegradable PVA/ Gelatin/ Curcumin membranes could be efficiently used as dressers for enhancing wound healing.","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"179 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88467497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-01DOI: 10.21608/djs.2021.197873
Eman H. F. Abd El-Zaher, Alaa M. Abou Zied, Youssef Mohamed, Faten M. A. Semana
sapidus Mushrooms have been used since centuries for the treatment of different diseases and efforts were made to isolate different compounds from the mushrooms responsible for medicinal activities. Among the mushroom genus Pleurotus is worldwide in distribution due to their medicinal properties such interventions ameliorate hepatic lipid profiles, reduce inflammation and oxidative stress, noticeable antioxidant, antiinflammatory, antimicrobial, antitumor and analgesic properties. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. The aim of current study is to improve of Exopolysaccharide production by Pleurotus sapidus through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett–Burman design indicated that; shaking, starch and incubation temperature are the most effective parameters. Box–Behnken RSM indicated that; the optimum concentration for each parameter was: 148.9 rpm for shaking, 30.1g/l starch and 25.2 oC for incubation temperature. After production, EPS was extracted, purified, identified using Fourier transform infrared (FTIR), Nuclear Magnetic Resonance (1HNMR) and High Performance Liquid Chromatography (HPLC). The result indicated that; it consisting of β-(1→3) glucans, β-(1→6) glucans branched, Chitin, Mannans and Galactans with different concentrations in EPS. Exopolysaccharides extracted from Pleurotus sapidus showed antitumor, antiviral, antibacterial, anticoagulant and antioxidant activity.
{"title":"THE ROLE OF EXOPOLYSACCHARIDE PRODUCED BY Pleurotus Sapidus AS MEDICAL AGENT","authors":"Eman H. F. Abd El-Zaher, Alaa M. Abou Zied, Youssef Mohamed, Faten M. A. Semana","doi":"10.21608/djs.2021.197873","DOIUrl":"https://doi.org/10.21608/djs.2021.197873","url":null,"abstract":"sapidus Mushrooms have been used since centuries for the treatment of different diseases and efforts were made to isolate different compounds from the mushrooms responsible for medicinal activities. Among the mushroom genus Pleurotus is worldwide in distribution due to their medicinal properties such interventions ameliorate hepatic lipid profiles, reduce inflammation and oxidative stress, noticeable antioxidant, antiinflammatory, antimicrobial, antitumor and analgesic properties. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. The aim of current study is to improve of Exopolysaccharide production by Pleurotus sapidus through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett–Burman design indicated that; shaking, starch and incubation temperature are the most effective parameters. Box–Behnken RSM indicated that; the optimum concentration for each parameter was: 148.9 rpm for shaking, 30.1g/l starch and 25.2 oC for incubation temperature. After production, EPS was extracted, purified, identified using Fourier transform infrared (FTIR), Nuclear Magnetic Resonance (1HNMR) and High Performance Liquid Chromatography (HPLC). The result indicated that; it consisting of β-(1→3) glucans, β-(1→6) glucans branched, Chitin, Mannans and Galactans with different concentrations in EPS. Exopolysaccharides extracted from Pleurotus sapidus showed antitumor, antiviral, antibacterial, anticoagulant and antioxidant activity.","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78399781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-01DOI: 10.21608/DJS.2021.190753
M. Shihab, A. Elbadawy, M. Afify, N. El-siragy
Radiofrequency capacitively coupled plasma is studied theoretically using a Particle-in-Cell code. For He discharge, the timeaveraged sheaths are in the range of few centimeters. The sheath potential, ion, and electron energy and angular distributions, discharge current, and dissipated power depend on the driven potentials and frequencies. Increasing the amplitude of the high radio frequencies increases the bulk density and the sheath potential and, consequently, increases the plasma processing rate. Increasing the intermediate radio frequency amplitude allows a wider sheath with a broad ion energy distribution and a narrower ion angular distribution. Changing the amplitude and the phase shift between driven frequencies provide different energies and angular distribution allowing performing various processes. The interplay between the sheath and bulk dynamics in the intermediate radiofrequency regime and the high-frequency regime may excite harmonics in the discharge current.
{"title":"Kinetic Simulation of He radio frequency capacitively coupled plasma","authors":"M. Shihab, A. Elbadawy, M. Afify, N. El-siragy","doi":"10.21608/DJS.2021.190753","DOIUrl":"https://doi.org/10.21608/DJS.2021.190753","url":null,"abstract":"Radiofrequency capacitively coupled plasma is studied theoretically using a Particle-in-Cell code. For He discharge, the timeaveraged sheaths are in the range of few centimeters. The sheath potential, ion, and electron energy and angular distributions, discharge current, and dissipated power depend on the driven potentials and frequencies. Increasing the amplitude of the high radio frequencies increases the bulk density and the sheath potential and, consequently, increases the plasma processing rate. Increasing the intermediate radio frequency amplitude allows a wider sheath with a broad ion energy distribution and a narrower ion angular distribution. Changing the amplitude and the phase shift between driven frequencies provide different energies and angular distribution allowing performing various processes. The interplay between the sheath and bulk dynamics in the intermediate radiofrequency regime and the high-frequency regime may excite harmonics in the discharge current.","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87973946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-01DOI: 10.21608/djs.2021.192031
M. Ghoneim, Mohamed M. Abu Anbar, D. H. Ramadan, Ahmed E. Masoud
Geothermobarometry, Mineral Chemistry, Wadi Seih, South Sinai, Egypt, ArabianNubian Shield The present work deals with the gabbroic intrusion in Wadi Seih area as a part of the Neoproterozoic Pan-African basement in the southwestern part of Sinai, Egypt. It comprises as small plutons intruded into Seih metamorphic belt. The intrusions comprise hornblende gabbros, anorthositic gabbros, and sometimes occur as coarse-grained gabbros (appenites). These plutons belong to what is known throughout Egypt as Younger Gabbros. The present study includes petrography, whole-rock geochemistry, mineral chemistry, and geothermobarometry. Geochemically, the gabbroic intrusions are derived from tholeiitic magma with minor calc-alkaline affinity. They have the chemical signature of subduction-related arc magmatism formed at an active convergent plate margin by 15–30% of partial melting of garnet lherzolite and to a minor extent of spinel-garnet lherzolite sources, modified by fluids related to a subducting slab. Mineral chemistry indicates that the gabbroic rocks crystallized at a pressure between 2.9 and 4.4 kb and a temperature between 590 °C and 700 °C.
{"title":"Petrogenesis and tectonic implications of Seih Syn-tectonic gabbroic intrusion, South Sinia, Egypt: Insights from whole-rock geochemistry, mineral chemistry and P-T estimate","authors":"M. Ghoneim, Mohamed M. Abu Anbar, D. H. Ramadan, Ahmed E. Masoud","doi":"10.21608/djs.2021.192031","DOIUrl":"https://doi.org/10.21608/djs.2021.192031","url":null,"abstract":"Geothermobarometry, Mineral Chemistry, Wadi Seih, South Sinai, Egypt, ArabianNubian Shield The present work deals with the gabbroic intrusion in Wadi Seih area as a part of the Neoproterozoic Pan-African basement in the southwestern part of Sinai, Egypt. It comprises as small plutons intruded into Seih metamorphic belt. The intrusions comprise hornblende gabbros, anorthositic gabbros, and sometimes occur as coarse-grained gabbros (appenites). These plutons belong to what is known throughout Egypt as Younger Gabbros. The present study includes petrography, whole-rock geochemistry, mineral chemistry, and geothermobarometry. Geochemically, the gabbroic intrusions are derived from tholeiitic magma with minor calc-alkaline affinity. They have the chemical signature of subduction-related arc magmatism formed at an active convergent plate margin by 15–30% of partial melting of garnet lherzolite and to a minor extent of spinel-garnet lherzolite sources, modified by fluids related to a subducting slab. Mineral chemistry indicates that the gabbroic rocks crystallized at a pressure between 2.9 and 4.4 kb and a temperature between 590 °C and 700 °C.","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83930319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-01DOI: 10.21608/djs.2021.195252
Mayssoun. M. Mourad, Y. Abdou, M. Berber, F. Elhussiny
Nano tungsten oxide polymer composites ; Shielding; Gamma radiation protection Lead is a traditional shielding material for gamma radiation protection, in spite of its disadvantages such as high density, toxicity and high cost. New shielding materials that have the same effective attenuation and have more advantages are studied. The aim of this work is to prepare new nano-composite material shield (nontoxic, light, and environmental friendly) that minimize the radiation hazards. Polymers are safe and light materials but on their own aren't effective in radiation shielding. The idea is to use a high-density metal which is the most effective materials in shielding with polymers which have the property of lightness and flexibility. A new nano-composite will be produced with improved advantage in shielding martials.
{"title":"USING NANO TUNGSTEN OXIDE POLYMER COMPOSITE AS A GAMMA RADIATION SHIELDING","authors":"Mayssoun. M. Mourad, Y. Abdou, M. Berber, F. Elhussiny","doi":"10.21608/djs.2021.195252","DOIUrl":"https://doi.org/10.21608/djs.2021.195252","url":null,"abstract":"Nano tungsten oxide polymer composites ; Shielding; Gamma radiation protection Lead is a traditional shielding material for gamma radiation protection, in spite of its disadvantages such as high density, toxicity and high cost. New shielding materials that have the same effective attenuation and have more advantages are studied. The aim of this work is to prepare new nano-composite material shield (nontoxic, light, and environmental friendly) that minimize the radiation hazards. Polymers are safe and light materials but on their own aren't effective in radiation shielding. The idea is to use a high-density metal which is the most effective materials in shielding with polymers which have the property of lightness and flexibility. A new nano-composite will be produced with improved advantage in shielding martials.","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89806602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.21608/djs.2020.147776
H. Harraz, M. Hamdy, A. A. Abu El-Ela, Islam M. El-Hoseiny
{"title":"ASSESSMENT OF RAW MATERIALS FOR CEMENT INDUSTRY IN EL SUKHUNA-ZAAFRANA AREA, EASTERN DESERT, EGYPT","authors":"H. Harraz, M. Hamdy, A. A. Abu El-Ela, Islam M. El-Hoseiny","doi":"10.21608/djs.2020.147776","DOIUrl":"https://doi.org/10.21608/djs.2020.147776","url":null,"abstract":"","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83834531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/DJS.2020.139224
E. Ammar, A. Emsimir
In this paper a suggested algorithm to solve fully rough multi-objectiveinteger linear programming problem [FRMOILP] is described. In orderto solve this problem and find rough value efficient solutions anddecision rough integer variables by the slice-sum method with thebranch and bound technique, we will use two methods, the first one isthe method of weights and the second is e- Constraint method. The basicidea of the computational phase of the algorithm is based onconstructing two LP problems with interval coefficients, and then to fourcrisp LPs. In addition to determining the weights and the values of e-constraint. Also, we reviewed some of the advantages and disadvantagesfor them. We used integer programming because many linearprogramming problems require that the decision variables are integers.Also, rough intervals (RIs) are very important to tackle the uncertaintyand imprecise data in decision making problems. In addition, theproposed algorithm enables us to search for the efficient solution in thelargest range of possible solutions range. Also, we obtain N suggestedsolutions and which enables the decision maker to choose the bestdecisions. Finally, two numerical examples are given to clarify theobtained results in the paper.
{"title":"On Solving Fully Rough Multi-Objective Integer Linear Programming Problems","authors":"E. Ammar, A. Emsimir","doi":"10.21608/DJS.2020.139224","DOIUrl":"https://doi.org/10.21608/DJS.2020.139224","url":null,"abstract":"In this paper a suggested algorithm to solve fully rough multi-objectiveinteger linear programming problem [FRMOILP] is described. In orderto solve this problem and find rough value efficient solutions anddecision rough integer variables by the slice-sum method with thebranch and bound technique, we will use two methods, the first one isthe method of weights and the second is e- Constraint method. The basicidea of the computational phase of the algorithm is based onconstructing two LP problems with interval coefficients, and then to fourcrisp LPs. In addition to determining the weights and the values of e-constraint. Also, we reviewed some of the advantages and disadvantagesfor them. We used integer programming because many linearprogramming problems require that the decision variables are integers.Also, rough intervals (RIs) are very important to tackle the uncertaintyand imprecise data in decision making problems. In addition, theproposed algorithm enables us to search for the efficient solution in thelargest range of possible solutions range. Also, we obtain N suggestedsolutions and which enables the decision maker to choose the bestdecisions. Finally, two numerical examples are given to clarify theobtained results in the paper.","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80177789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-01DOI: 10.21608/DJS.2020.139230
R. Ramadan, H. Khairy, M. Halawa, A. Haider
Text Cylindrotheca closterium alga belongs to family Bacillariaceae, orderBacillariales, class Bacillariophyceae and phylum Bacillariophyta. In this studyCylindrotheca closterium alga was isolated from Alexandria coastal region(Eastern Harbor) using two methods: dilution method and streak and sprayplating technique. Isolated algal cells were cultured on Guillard F2 liquidmedium. Morphological identification was carried out by light microscope andscanning electric microscope. Algal cells were found to be wrapped by theraphe canal of valves, which is a typical characteristic of Cylindrothecaclosterium shape. The cells contained two plastids lying next to each otheralong the longitudinal axis of the cell and apprised to one side of the cell withlobes extending onto the other sides, and the nucleus centrally in between.However, previous studies indicated some morphological variations during thelife cycle of the alga.Genetic identification of Cylindrotheca closterium wascarried out using ribulose-bisphosphate carboxylase gene (rbcL) located in theplastids as molecular marker. Specific PCR primers were used to amplify rbcLgene fragment from total genomic DNA extracted from Cylindrothecaclosterium cells. Amplified rbcL (1177 bp) gene fragment was purified andsequenced. Nucleotide sequence was compared with Cylindrotheca sequencesavailable in the GenBank. The Genetic distances and multi alignments werecomputed by Pairwise Distance method using Clusteral W software analysis.The nucleotide sequence of amplified rbcL gene showed varied identitypercentages (81, 80 and 78%) with other related sequences in the GenBank.This could be correlated to the observed morphological variations. Previousstudies indicated high sequence divergences of rbcL gene amongCylindrotheca isolates, and numerous nucleotide variations of rbcL genecaused relatively few variation of deduced amino acid sequence. These resultsmay indicate that Cylindrotheca closterium is a species complex as waspreviously evidenced by the variations of rbcL gene.
{"title":"Isolation and genetic identification of Cylindrotheca closterium algae from Alexandria coastal region","authors":"R. Ramadan, H. Khairy, M. Halawa, A. Haider","doi":"10.21608/DJS.2020.139230","DOIUrl":"https://doi.org/10.21608/DJS.2020.139230","url":null,"abstract":"Text Cylindrotheca closterium alga belongs to family Bacillariaceae, orderBacillariales, class Bacillariophyceae and phylum Bacillariophyta. In this studyCylindrotheca closterium alga was isolated from Alexandria coastal region(Eastern Harbor) using two methods: dilution method and streak and sprayplating technique. Isolated algal cells were cultured on Guillard F2 liquidmedium. Morphological identification was carried out by light microscope andscanning electric microscope. Algal cells were found to be wrapped by theraphe canal of valves, which is a typical characteristic of Cylindrothecaclosterium shape. The cells contained two plastids lying next to each otheralong the longitudinal axis of the cell and apprised to one side of the cell withlobes extending onto the other sides, and the nucleus centrally in between.However, previous studies indicated some morphological variations during thelife cycle of the alga.Genetic identification of Cylindrotheca closterium wascarried out using ribulose-bisphosphate carboxylase gene (rbcL) located in theplastids as molecular marker. Specific PCR primers were used to amplify rbcLgene fragment from total genomic DNA extracted from Cylindrothecaclosterium cells. Amplified rbcL (1177 bp) gene fragment was purified andsequenced. Nucleotide sequence was compared with Cylindrotheca sequencesavailable in the GenBank. The Genetic distances and multi alignments werecomputed by Pairwise Distance method using Clusteral W software analysis.The nucleotide sequence of amplified rbcL gene showed varied identitypercentages (81, 80 and 78%) with other related sequences in the GenBank.This could be correlated to the observed morphological variations. Previousstudies indicated high sequence divergences of rbcL gene amongCylindrotheca isolates, and numerous nucleotide variations of rbcL genecaused relatively few variation of deduced amino acid sequence. These resultsmay indicate that Cylindrotheca closterium is a species complex as waspreviously evidenced by the variations of rbcL gene.","PeriodicalId":11368,"journal":{"name":"Delta Journal of Science","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80841138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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