Pub Date : 2001-01-01DOI: 10.3109/10425170109047566
L. Keith, C. Bender
The algT-muc gene cluster (rpoE operon) is important for alginate production and survival during environmental stress in Pseudomonas syringae. The algT-muc operon was cloned and sequenced from P. syringae to determine whether the organization of this gene cluster was conserved in this Plant Pathogen. Interestingly, analysis of the algT-muc region in P. syringae revealed a uniclue arrangement when comDared to other bacteria ahd lacked a mucC homolope: The relative importance of the mucC gene in the algT (rpoE) operon of various bacterial species is discussed.
{"title":"Genetic Divergence in the algT-muc Operon Controlling Alginate Biosynthesis and Response to Environmental Stress in Pseudornonas syringae","authors":"L. Keith, C. Bender","doi":"10.3109/10425170109047566","DOIUrl":"https://doi.org/10.3109/10425170109047566","url":null,"abstract":"The algT-muc gene cluster (rpoE operon) is important for alginate production and survival during environmental stress in Pseudomonas syringae. The algT-muc operon was cloned and sequenced from P. syringae to determine whether the organization of this gene cluster was conserved in this Plant Pathogen. Interestingly, analysis of the algT-muc region in P. syringae revealed a uniclue arrangement when comDared to other bacteria ahd lacked a mucC homolope: The relative importance of the mucC gene in the algT (rpoE) operon of various bacterial species is discussed.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"125 1","pages":"125 - 129"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76700772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109084458
J. Kitanaka, Xiao‐Bing Wang, N. Kitanaka, C. Hembree, G. Uhl
The functional significance of heterotrimeric guanine nucleotide binding protein (G protein) for the many physiological processes including the molecular mechanisms of drug addiction have been described. In investigating the changes of mRNA expression after acute psychostimulant administration, we previously identified a cDNA encoding a G protein pi subunit (Gpi) that was increased up to four-fold in certain brain regions after administration of psychostimulants. The mouse Gpi gene (the mouse genetic symbol, GNB1) was mapped to chromosome 4, but little was known of its genetic features. To characterize the GNB1 gene further, we have cloned and analyzed the genomic structures of the mouse GNB1 gene and its homologous sequences. The GNB1 gene spans at least 50 kb, and consists of 12 exons and 11 introns. The exon/intron boundaries were determined and found to follow the GT/AG rule. Exons 3-11 encode the Gpi protein, and the exon 2 is an alternative, resulting in putative two splicing variants. Although intron 11 is additional for GNB1 compared with GNB2 and GNB3, the intron positions within the protein coding region of GNB1, GNB2 and GNB3 are identical, suggesting that GNB1 should have diverged from the ancestral gene family earlier than the genes for GNB2 and GNB3. We also found the 5′-truncated processed pseudogenes with 71-89% similarities to GNB1 mRNA sequence, suggesting that the truncated cDNA copies, which have been reverse-transcribed from a processed mRNA for GNB1, might have been integrated into several new locations in the mouse genome.
{"title":"Genomic Organization of the Murine G Protein (3 Subunit Genes and Related Processed Pseudogenes","authors":"J. Kitanaka, Xiao‐Bing Wang, N. Kitanaka, C. Hembree, G. Uhl","doi":"10.3109/10425170109084458","DOIUrl":"https://doi.org/10.3109/10425170109084458","url":null,"abstract":"The functional significance of heterotrimeric guanine nucleotide binding protein (G protein) for the many physiological processes including the molecular mechanisms of drug addiction have been described. In investigating the changes of mRNA expression after acute psychostimulant administration, we previously identified a cDNA encoding a G protein pi subunit (Gpi) that was increased up to four-fold in certain brain regions after administration of psychostimulants. The mouse Gpi gene (the mouse genetic symbol, GNB1) was mapped to chromosome 4, but little was known of its genetic features. To characterize the GNB1 gene further, we have cloned and analyzed the genomic structures of the mouse GNB1 gene and its homologous sequences. The GNB1 gene spans at least 50 kb, and consists of 12 exons and 11 introns. The exon/intron boundaries were determined and found to follow the GT/AG rule. Exons 3-11 encode the Gpi protein, and the exon 2 is an alternative, resulting in putative two splicing variants. Although intron 11 is additional for GNB1 compared with GNB2 and GNB3, the intron positions within the protein coding region of GNB1, GNB2 and GNB3 are identical, suggesting that GNB1 should have diverged from the ancestral gene family earlier than the genes for GNB2 and GNB3. We also found the 5′-truncated processed pseudogenes with 71-89% similarities to GNB1 mRNA sequence, suggesting that the truncated cDNA copies, which have been reverse-transcribed from a processed mRNA for GNB1, might have been integrated into several new locations in the mouse genome.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"39 1","pages":"345 - 354"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79467559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109080771
A. Hernández, J. Copa-Patiño, J. Soliveri
The xylanase gene xysA of Streptomyces halstedii JM8 was used to isolate a DNA fragment from a gene library of Pstf-digested chromosomal DNA of the lignocellulolytic actinomycete Streptomyces chattanoogensis CECT-3336. Nucleotide sequence analysis revealed a gene (xln23) encoding a bifunctional multi-modular enzyme bearing two independent xylanase and a-L-arabinofuranosidase domains separated by a Ser/Gly-rich linker. The N terminus of the predicted protein showed high homology to family F xylanases. The C terminus was homologous to amino acid sequences found in enzymes included in the glycosyl hydrolase family 62 and, in particular, to those of a-L-arabinofuranosidase AbsB from Streptomyces livi-dans. PCR and RT-PCR experiments showed that the nucleotide sequences corresponding to each domain are arranged as expected on the chromosomal DNA and that they are cotranscribed. To our knowledge, this is the first description of xylanase and arabinofuranosidase domains in a same open reading frame.
利用halstedstreptomyces chattanoogensis CECT-3336基因文库中的木聚糖酶基因xysA，分离了halstedstreptomyces JM8的木聚糖酶基因片段。核苷酸序列分析显示，该基因(xln23)编码一种双功能多模块酶，具有两个独立的木聚糖酶和a- l -阿拉伯糖苷酶结构域，由一个富含丝氨酸/甘氨酸的连接体分隔。预测蛋白的N端与F家族木聚糖酶具有较高的同源性。C端与糖基水解酶家族62中酶的氨基酸序列同源，特别是与链霉菌(Streptomyces livis -dans)的a- l -阿拉伯糖醛酸苷酶(a-L-arabinofuranosidase AbsB的氨基酸序列同源。PCR和RT-PCR实验表明，每个结构域对应的核苷酸序列在染色体DNA上的排列与预期一致，并且它们是共转录的。据我们所知，这是第一次在同一个开放阅读框中描述木聚糖酶和阿拉伯糖铀苷酶结构域。
{"title":"xln23 from Streptomyces chattanoogensis UAH23 Encodes a Putative Enzyme with Separate Xylanase and Arabinofuranosidase Catalytic Domains","authors":"A. Hernández, J. Copa-Patiño, J. Soliveri","doi":"10.3109/10425170109080771","DOIUrl":"https://doi.org/10.3109/10425170109080771","url":null,"abstract":"The xylanase gene xysA of Streptomyces halstedii JM8 was used to isolate a DNA fragment from a gene library of Pstf-digested chromosomal DNA of the lignocellulolytic actinomycete Streptomyces chattanoogensis CECT-3336. Nucleotide sequence analysis revealed a gene (xln23) encoding a bifunctional multi-modular enzyme bearing two independent xylanase and a-L-arabinofuranosidase domains separated by a Ser/Gly-rich linker. The N terminus of the predicted protein showed high homology to family F xylanases. The C terminus was homologous to amino acid sequences found in enzymes included in the glycosyl hydrolase family 62 and, in particular, to those of a-L-arabinofuranosidase AbsB from Streptomyces livi-dans. PCR and RT-PCR experiments showed that the nucleotide sequences corresponding to each domain are arranged as expected on the chromosomal DNA and that they are cotranscribed. To our knowledge, this is the first description of xylanase and arabinofuranosidase domains in a same open reading frame.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"297 1","pages":"167 - 177"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79598907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109041334
S. Spada, Yann Gibert, J. Pembroke, J. Wall
Screening of a Thermus thermophilus genomic library led to the identification of a homologue of the ylmE gene. ylmE is highly conserved in widely divergent organisms from prokaryotes to mammals, suggesting an important, albeit currently unknown, cellular function. The 633 bp gene has a GC content of 69.2% overall and 90% in the third nucleotide position, while the gene product is predicted to be a soluble cytoplasmic protein of 23441 Da. It belongs to a family of conserved proteins of unknown function and exhibits amino acid identities ranging from 45% to 28% to the Aquifex aeolicus and Saccharomyces cerevisiae family members, respectively. We speculate that the gene product may be involved in a cellular stress response in T. thermophilus.
{"title":"Isolation and Characterisation of the ylmE Homologue of Thermus thermophilus","authors":"S. Spada, Yann Gibert, J. Pembroke, J. Wall","doi":"10.3109/10425170109041334","DOIUrl":"https://doi.org/10.3109/10425170109041334","url":null,"abstract":"Screening of a Thermus thermophilus genomic library led to the identification of a homologue of the ylmE gene. ylmE is highly conserved in widely divergent organisms from prokaryotes to mammals, suggesting an important, albeit currently unknown, cellular function. The 633 bp gene has a GC content of 69.2% overall and 90% in the third nucleotide position, while the gene product is predicted to be a soluble cytoplasmic protein of 23441 Da. It belongs to a family of conserved proteins of unknown function and exhibits amino acid identities ranging from 45% to 28% to the Aquifex aeolicus and Saccharomyces cerevisiae family members, respectively. We speculate that the gene product may be involved in a cellular stress response in T. thermophilus.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"205 1","pages":"507 - 514"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76037176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109024999
D. Irwin
In human and rat, tissue-specific proteolytic processing of identical proglucagon precursors yield tissue-specific proglucagon-derived peptides. In contrast, in many non-mammalian vertebrates alternative mRNA splicing yields different proglucagon precursors in different tissues. Thus alternative mRNA splicing, in part, limits the choices of proglucagon-derived peptides that can be produced by proteolytic processing. Stomach proglucagon mRNAs from the rainbow trout and Xenopus laevis were found not to encode the proglucagon-derived peptide glucagon-like peptide 2 (GLP-2). To determine if the absence of GLP-2 was a general feature of stomach proglucagons we isolated and characterized proglucagon cDNAs from the stomach and the pancreas of the dog, a mammal that expresses the proglucagon gene in the stomach. A major proglucagon transcript of about 1100 bases and a minor transcript of about 800 bases were identified in both stomach and pancreas. The coding sequences of both the stomach and pancreatic proglucagon transcripts were identical. Therefore, tissue-specific proteolytic processing, and not alternative mRNA splicing, must regulate the production of tissue-specific proglucagon-derived peptides from the stomach of the dog.
{"title":"cDNA Cloning of Proglucagon from the Stomach and Pancreas of the Dog","authors":"D. Irwin","doi":"10.3109/10425170109024999","DOIUrl":"https://doi.org/10.3109/10425170109024999","url":null,"abstract":"In human and rat, tissue-specific proteolytic processing of identical proglucagon precursors yield tissue-specific proglucagon-derived peptides. In contrast, in many non-mammalian vertebrates alternative mRNA splicing yields different proglucagon precursors in different tissues. Thus alternative mRNA splicing, in part, limits the choices of proglucagon-derived peptides that can be produced by proteolytic processing. Stomach proglucagon mRNAs from the rainbow trout and Xenopus laevis were found not to encode the proglucagon-derived peptide glucagon-like peptide 2 (GLP-2). To determine if the absence of GLP-2 was a general feature of stomach proglucagons we isolated and characterized proglucagon cDNAs from the stomach and the pancreas of the dog, a mammal that expresses the proglucagon gene in the stomach. A major proglucagon transcript of about 1100 bases and a minor transcript of about 800 bases were identified in both stomach and pancreas. The coding sequences of both the stomach and pancreatic proglucagon transcripts were identical. Therefore, tissue-specific proteolytic processing, and not alternative mRNA splicing, must regulate the production of tissue-specific proglucagon-derived peptides from the stomach of the dog.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"265 2","pages":"253 - 260"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91456328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109041330
L. Harrier
The Glomus mosseae 3-phosphogly cerate kinase (GmPGK) gene promoter has been isolated from a phage genomic library and represents one of the few promoter elements to be isolated and analysed from these symbiotic fungi. The analysis revealed the presence of several motifs which are found in the promoter region of other fungal PGK genes. In particular, DNA sequences homologous to segments of the S. cer-evisiae and Rhizopus niveus upstream activating elements (UAS). The importance of these UAS sequences in regulating carbon source in PGK genes is known and the presence of two carbon source regulated UAS sequences in the GmPGK gene promoter and its role in the biology of AM fungi is discussed briefly.
{"title":"Isolation and Sequence Analysis of the Arbuscular Mycorrhizal Fungus Glomus mosseae (Nicol & Gerd.) Gerdemann & Trappe 3-Phosphoglycerate Kinase (PGK) Gene Promoter Region","authors":"L. Harrier","doi":"10.3109/10425170109041330","DOIUrl":"https://doi.org/10.3109/10425170109041330","url":null,"abstract":"The Glomus mosseae 3-phosphogly cerate kinase (GmPGK) gene promoter has been isolated from a phage genomic library and represents one of the few promoter elements to be isolated and analysed from these symbiotic fungi. The analysis revealed the presence of several motifs which are found in the promoter region of other fungal PGK genes. In particular, DNA sequences homologous to segments of the S. cer-evisiae and Rhizopus niveus upstream activating elements (UAS). The importance of these UAS sequences in regulating carbon source in PGK genes is known and the presence of two carbon source regulated UAS sequences in the GmPGK gene promoter and its role in the biology of AM fungi is discussed briefly.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"39 1","pages":"463 - 473"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87151485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109041335
I. Wu, Marsha A. Mosesx
Using modified differential display, a gene fragment was identified as being over-expressed in human vascularized breast carcinoma when compare to its neighboring normal tissue. The differentially expressed pattern was confirmed by quantitative RT-PCR. Full-length cDNA was then cloned by both 3′-end RACE and 5′-end RACE. Analysis of the full-length cDNA of this gene reveals that this cDNA encodes an open reading frame of 615 bp, which is highly homologous to human protein phosphatase inhibitor-2, with 92% identity at the nucleotide level, and 89% identity at amino acid level. The results of this study suggest that this novel isoform of human protein phosphatase inhibitor-2 (nPPI-2) may be involved in the angiogenic switch during breast tumor development.
{"title":"Cloning of a cDNA Encoding an Isoform of Human Protein Phosphatase Inhibitor 2 from Vascularized Breast Tumor","authors":"I. Wu, Marsha A. Mosesx","doi":"10.3109/10425170109041335","DOIUrl":"https://doi.org/10.3109/10425170109041335","url":null,"abstract":"Using modified differential display, a gene fragment was identified as being over-expressed in human vascularized breast carcinoma when compare to its neighboring normal tissue. The differentially expressed pattern was confirmed by quantitative RT-PCR. Full-length cDNA was then cloned by both 3′-end RACE and 5′-end RACE. Analysis of the full-length cDNA of this gene reveals that this cDNA encodes an open reading frame of 615 bp, which is highly homologous to human protein phosphatase inhibitor-2, with 92% identity at the nucleotide level, and 89% identity at amino acid level. The results of this study suggest that this novel isoform of human protein phosphatase inhibitor-2 (nPPI-2) may be involved in the angiogenic switch during breast tumor development.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"26 1","pages":"515 - 518"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80163869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109080776
B. Blitvich, A. Rayms-keller, C. Blair, B. Beaty
The chaperonin containing t-complex polypeptide 1 (CCT) assists in the ATP-dependent folding and assembly of newly translated actin and tubulin in the eukaryotic cytosol. CCT is composed of eight different subunits, each encoded by an independent gene. In this report, we used RT-PCR amplification and 5′- and 3′-rapid amplification of cDNA ends (RACE) to determine the complete cDNA sequence of the CCT delta subunit from Aedes triseriatus mosquitoes. The CCT5 cDNA is 1936 nucleotides in length and encodes a putative 533 amino acid protein with a calculated molecular mass of 57 179 daltons and pi of 7.15. Hydrophobic residues comprise 39.8% of the amino acid sequence and putative motifs for ATP-binding and ATPase-activity are present. The amino acid sequence displays strong sequence similarity to Dro-sophila melanogaster (92%), human (85%), puffer fish (84%) and mouse (84%) counterparts. CCT8 mRNA was detected in both biosynthetically active (embryo-nating) and dormant (diapausing) Ae. triseriatus embryos by RT-PCR analysis.
{"title":"Complete cDNA and Deduced Amino Acid Sequence of the Chaperonin Containing T-Complex Polypeptide 1 (CCT) Delta Subunit from Aedes triseriatus Mosquitoes","authors":"B. Blitvich, A. Rayms-keller, C. Blair, B. Beaty","doi":"10.3109/10425170109080776","DOIUrl":"https://doi.org/10.3109/10425170109080776","url":null,"abstract":"The chaperonin containing t-complex polypeptide 1 (CCT) assists in the ATP-dependent folding and assembly of newly translated actin and tubulin in the eukaryotic cytosol. CCT is composed of eight different subunits, each encoded by an independent gene. In this report, we used RT-PCR amplification and 5′- and 3′-rapid amplification of cDNA ends (RACE) to determine the complete cDNA sequence of the CCT delta subunit from Aedes triseriatus mosquitoes. The CCT5 cDNA is 1936 nucleotides in length and encodes a putative 533 amino acid protein with a calculated molecular mass of 57 179 daltons and pi of 7.15. Hydrophobic residues comprise 39.8% of the amino acid sequence and putative motifs for ATP-binding and ATPase-activity are present. The amino acid sequence displays strong sequence similarity to Dro-sophila melanogaster (92%), human (85%), puffer fish (84%) and mouse (84%) counterparts. CCT8 mRNA was detected in both biosynthetically active (embryo-nating) and dormant (diapausing) Ae. triseriatus embryos by RT-PCR analysis.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"39 1","pages":"203 - 208"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73446376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109041336
S. Irie, Yin Li, H. Kanki, Tomoko Ohyama, L. Deaven, Stefan Someo, Taka-Aki Sato
Fas-associated phosphatase-1 (FAP-1) has been reported as a negative regulator of Fas-mediated signal transduction in human cancer cells. To obtain insights into the potential carcinogenesis of the FAP-1 gene, we investigated its transcriptional regulation in normal and cancerous cells. To identify the FAP-1 promoter sequences, we first isolated PI and cosmid clones that contained the regulatory region upstream from the FAP-1 gene by using the PCR products of 5′ rapid amplification of cDN A end (5′-RACE) as probes. Genomic analysis of positive clones revealed that the major FAP-1 mRNA was transcribed from its proximal promoter (pPRM) in all human cancer cell lines tested, but 1 additional large transcript derived from its distal promoter (dPRM) was found in the human colon cancer cell line DLD-1. This suggests that the FAP-1 gene may be aberrantly dysregulated in some types of human cancers, including colon carcinoma. Sequence analysis of the region upstream from the FAP-1 gene strongly suggests that the transcript of the FAP-1 gene may be controlled by a variety of transcriptional regulatory elements, including NF-κB, NF-IL6, and p53 in its 2 promoters. These results imply that the FAP-1 gene may be a target gene under the control of important apoptosis-related nuclear factors in human cancers.
{"title":"Identification of Two Fas-Associated Phosphatase-1 (FAP-1) Promoters in Human Cancer Cells","authors":"S. Irie, Yin Li, H. Kanki, Tomoko Ohyama, L. Deaven, Stefan Someo, Taka-Aki Sato","doi":"10.3109/10425170109041336","DOIUrl":"https://doi.org/10.3109/10425170109041336","url":null,"abstract":"Fas-associated phosphatase-1 (FAP-1) has been reported as a negative regulator of Fas-mediated signal transduction in human cancer cells. To obtain insights into the potential carcinogenesis of the FAP-1 gene, we investigated its transcriptional regulation in normal and cancerous cells. To identify the FAP-1 promoter sequences, we first isolated PI and cosmid clones that contained the regulatory region upstream from the FAP-1 gene by using the PCR products of 5′ rapid amplification of cDN A end (5′-RACE) as probes. Genomic analysis of positive clones revealed that the major FAP-1 mRNA was transcribed from its proximal promoter (pPRM) in all human cancer cell lines tested, but 1 additional large transcript derived from its distal promoter (dPRM) was found in the human colon cancer cell line DLD-1. This suggests that the FAP-1 gene may be aberrantly dysregulated in some types of human cancers, including colon carcinoma. Sequence analysis of the region upstream from the FAP-1 gene strongly suggests that the transcript of the FAP-1 gene may be controlled by a variety of transcriptional regulatory elements, including NF-κB, NF-IL6, and p53 in its 2 promoters. These results imply that the FAP-1 gene may be a target gene under the control of important apoptosis-related nuclear factors in human cancers.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"51 1","pages":"519 - 526"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80723913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2001-01-01DOI: 10.3109/10425170109084462
S. Matsumoto, S. Hayashi, K. Kitahara, J. Soejima
All S-RNases in apple contained one intron at the same location within the hypervariable region. They code for 225-228 amino acids, however, the length of introns is variable and divided into three groups. The intron sequences of some S-RNases within the same group showed an extremely high similarity as well as their coding sequences, suggesting that they were generated from the same origin.
{"title":"Genomic DNA Sequences Encoding Malus X domestica Borkh. “Akane”, “Delicious” and Malus transitoria S-RNases","authors":"S. Matsumoto, S. Hayashi, K. Kitahara, J. Soejima","doi":"10.3109/10425170109084462","DOIUrl":"https://doi.org/10.3109/10425170109084462","url":null,"abstract":"All S-RNases in apple contained one intron at the same location within the hypervariable region. They code for 225-228 amino acids, however, the length of introns is variable and divided into three groups. The intron sequences of some S-RNases within the same group showed an extremely high similarity as well as their coding sequences, suggesting that they were generated from the same origin.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"1 1","pages":"381 - 383"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81982270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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