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Sequence Variability of Nine Cytosolic Ascorbate Peroxidases in Polyploid Strawberry 多倍体草莓九种细胞质抗坏血酸过氧化物酶的序列变异
Pub Date : 2001-01-01 DOI: 10.3109/10425170109041331
Byung-Hyun Lee, J. Jo, W. Chung
Little is known about differential gene expression at the molecular level in polyploid plants. Here, we describe the molecular analysis of ApxSC (cytosolic ascorbate peroxidase from a polyploid strawberry) genes. Fifty-three cDNAs encoding ApxSC were isolated from a strawberry fruit cDNA library. These clones were categorized (i) into nine homologous (95 to 99 %) gene groups on the basis of their nucleotide sequences and (ii) into four groups of similar (>98%) polypeptides on the basis of their deduced amino acid sequences. Sequence variation among the gene groups was dispersed throughout the gene, while differences among the polypeptide groups were observed only at three amino acid positions (9, 63, and 233). These results imply that the ApxSC genes show co-dominant expression resulting from multiple alleles. This hypothesis is supported by genomic blots and primer extension analyses.
多倍体植物分子水平上的差异基因表达尚不清楚。在这里,我们描述了ApxSC(细胞质抗坏血酸过氧化物酶从多倍体草莓)基因的分子分析。从草莓果实cDNA文库中分离到53个编码ApxSC的cDNA。这些克隆根据核苷酸序列可分为9个同源基因群(95% ~ 99%),根据推导出的氨基酸序列可分为4个相似多肽群(>98%)。基因组之间的序列差异分散在整个基因中,而多肽组之间的差异仅在3个氨基酸位置(9、63和233)上观察到。这些结果表明,ApxSC基因表现出多等位基因的共显性表达。这一假设得到了基因组印迹和引物扩展分析的支持。
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引用次数: 5
The Gene and Pseudogenes of Cbx3/mHPlγ Cbx3/mHPlγ的基因和假基因
Pub Date : 2001-01-01 DOI: 10.3109/10425170109080769
David O. Jones, M. Mattei, D. Horsley, I. Cowell, Prim B. Singh
The HP1 class of chromobox (Cbx) genes encode an evolutionarily conserved family of proteins involved in the packaging of chromosomal domains into a repressive heterochromatic state. The murine Cbx5, Cbxl and Cbx3 genes encode the three mouse HP1 proteins, mHPlot, -β and -γ respectively. Here, we report the cloning of the mouse Cbx31 HPlγ gene and the chromosomal localisation of Cbx3 and three Cfo3-related pseudogenes. The Cbx3 structural gene is located on mouse Chromosome 6, close to the Hoxa cluster. Two Cbx3 processed pseudogenes are separated by just 300 bp and are arranged in a head-to-tail configuration on Chromosome 13 while a third pseu-dogene is found on mouse Chromosome 4. The genomic intron-exon arrangement of Cbx3 is different from the conserved organisation of three other mammalian HP1 genes, Cbxl (mHPip), CBX3 (hHPly), and Cbx5 (mHPla) in that Cbx3 lacks an intron that is present in the others.
HP1类染色体盒(Cbx)基因编码一个进化上保守的蛋白质家族,该家族参与将染色体结构域包装成抑制异色状态。小鼠Cbx5、Cbxl和Cbx3基因分别编码三种小鼠HP1蛋白mHPlot、-β和-γ。在这里,我们报道了小鼠Cbx31 HPlγ基因的克隆以及Cbx3和三个cfo3相关假基因的染色体定位。Cbx3结构基因位于小鼠6号染色体上,靠近Hoxa簇。两个Cbx3处理的假基因仅相隔300 bp,在13号染色体上以首尾相连的结构排列,而在小鼠4号染色体上发现了第三个假基因。Cbx3的基因组内含子-外显子排列与其他三个哺乳动物HP1基因Cbxl (mHPip), Cbx3 (hHPly)和Cbx5 (mHPla)的保守组织不同,Cbx3缺乏存在于其他基因中的内含子。
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引用次数: 7
Characterization of the Region Encompassing the Human Lysyl Oxidase Locus 人类赖氨酸氧化酶基因座周围区域的特征
Pub Date : 2001-01-01 DOI: 10.3109/10425170109024996
R. P. Martins, A. Ujfalusi, K. Csiszȧr, S. Krawetz
A 46,823 bp region of human chromosome 5q23.1 encompassing the seven-exon lysyl oxidase gene was characterized at the primary sequence level. Approximately 17.4% of this region is comprised of repetitive elements. The gene colocalizes with microsatellite marker D5S467. It is flanked by two candidate nuclear matrix association regions (MARs). The 5′ MAR centered at position 12,500 is of the AT-rich and curved DNA class. This is followed by a large CpG island containing fifty-seven putative regulatory elements which extend from just upstream of exon 1 to intron 2. The larger 3′ MAR, spans position 35,050–39,750 and is characterized by a TG-rich kinked structure that also contains a topoisomerase II binding site. Based on these results model of the transcriptional regulation of the lysy/oxidase gene is presented.
人类5q23.1染色体上包含赖氨酸氧化酶基因的46,823 bp区域在一级序列水平上被鉴定。大约17.4%的区域由重复元素组成。该基因与微卫星标记D5S467共定位。它的两侧有两个候选核矩阵结合区(MARs)。以12,500为中心的5 ' MAR是富含at和弯曲的DNA类。随后是一个大的CpG岛,包含57个假定的调控元件,从外显子1上游延伸到内含子2。较大的3 ' MAR,跨越位置35,050-39,750,其特征是具有富含tg的扭结结构,也包含拓扑异构酶II结合位点。在此基础上,提出了酶/氧化酶基因的转录调控模型。
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引用次数: 2
Short Communication: Sequencing and Chromosomal Assignment of the Rat Endothelial-derived Lipase Gene (Lipg) 短通信:大鼠内皮源性脂肪酶基因(Lipg)的测序和染色体分配
Pub Date : 2001-01-01 DOI: 10.3109/10425170109025004
A. Bonné, M. D. den Bieman, H. V. van Lith, Bert F.M. van Zutphen
Part of the nucleotide sequence of the Lipg gene in the rat was established using primers based on the mRNA sequence described in the mouse. The rat intron sequence served as a template for designing primers for the specific amplification of rat Lipg. A rat-hamster radiation hybrid (RH) panel was used for chromosomal assignment of the rat Lipg gene. The Lipg gene was found to be located on rat chromosome 18 in the vicinity of the marker D18Mitll; a region reported to be homologous with both human and mouse chromosome 18.
根据小鼠的mRNA序列,用引物建立了大鼠Lipg基因的部分核苷酸序列。大鼠内含子序列可作为设计大鼠Lipg特异性扩增引物的模板。采用大鼠-仓鼠辐射杂交(RH)模型对大鼠Lipg基因进行染色体定位。Lipg基因位于大鼠18号染色体D18Mitll标记附近;一个据报道与人类和小鼠18号染色体同源的区域。
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引用次数: 1
Molecular Cloning and Sequence Analysis of the Manganese Catalase Gene from Thermoleophilum album NM 嗜热菌锰过氧化氢酶基因的克隆及序列分析
Pub Date : 2001-01-01 DOI: 10.3109/10425170109084467
Krisana Phucharoen, Yuuki Takenaka, T. Shinozawa
The manganese catalase gene (mnct) from Thermoleophilum album NM, a thermophilic bacterium, was cloned and its nucleotide sequence was analyzed. The gene consists of 885 bp (65.4% GC content) encoding 294 amino acids with a molecular mass of 32,500 Da. The deduced amino acid sequence shows similarities to those of Thermus species strain YS 8-13 (a thermophilic bacterium) and Bacillus halodurans (an alkaliphilic bacterium) with 61 and 54% identities, respectively.
从嗜热细菌Thermoleophilum album NM中克隆了锰过氧化氢酶基因(mnct),并对其核苷酸序列进行了分析。该基因全长885 bp (GC含量65.4%),编码294个氨基酸,分子量为32,500 Da。所得氨基酸序列与嗜热菌YS 8-13和嗜碱杆菌YS 8-13的氨基酸序列相似,同源性分别为61%和54%。
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引用次数: 4
Nucleotide Sequence Analysis of a Transforming Gene Isolated from Nasopharyngeal Carcinoma Cell Line CNE2: an Aberrant Human Immunoglobulin Kappa Light Chain Which Lacks Variable Region 鼻咽癌细胞系CNE2:缺乏可变区异常人免疫球蛋白Kappa轻链转化基因的核苷酸序列分析
Pub Date : 2001-01-01 DOI: 10.3109/10425170109084456
Ming Li, Wei Ren, X. Weng, W. Liao, L. Xia, Xiyun Deng, Ya Cao
A transforming gene, designated Tx, was isolated from a human nasopharyngeal carcinoma (NPC) cell line CNE2 by transfection and molecular cloning techniques. The Tx gene was analyzed using computer-based bioinformatics and compared with the known sequences in EMBL and GenBank databases. We found that Tx contains human immunoglobulin kappa light chain constant region, five intact joining regions J1-J5, five recombination signal sequences and an N-segment besides classic regulatory sequences such as TATA boxes, CAAT boxes, poly A signals, etc. Interestingly, Tx also contains several binding sites for nuclear transcription factors such as NF-KB, NF-IL6, TFIID, etc. In conclusion, there are only several base pairs mutations or deletions compared with normal Ig K JC gDNA fragment. In all, Tx is an aberrant human immunoglobulin kappa light chain that contains the constant region, five joining regions, which lacks the variable regions.
通过转染和分子克隆技术,从人鼻咽癌(NPC)细胞系CNE2中分离到一个转化基因Tx。利用计算机生物信息学对Tx基因进行分析,并与EMBL和GenBank数据库中的已知序列进行比较。我们发现,Tx除TATA盒、CAAT盒、poly A信号等经典调控序列外,还含有人免疫球蛋白kappa轻链常数区、5个完整连接区J1-J5、5个重组信号序列和1个n段。有趣的是,Tx还含有NF-KB、NF-IL6、TFIID等核转录因子的几个结合位点。总之,与正常的Ig K JC dna片段相比,只有几个碱基对突变或缺失。总之,Tx是一个异常的人免疫球蛋白kappa轻链,包含恒定区,五个连接区,缺乏可变区。
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引用次数: 14
Molecular Cloning, Sequencing Analysis and Expression of the Catalase-Peroxidase Gene from Halobacterium Salinarum 盐盐杆菌过氧化氢酶-过氧化物酶基因的克隆、测序分析及表达
Pub Date : 2001-01-01 DOI: 10.3109/10425170109042049
Shinong Long, M. Salin
The gene encoding catalase-peroxidase was cloned from chromosomal DNA from the Archaea, Halobacterium salinarum. The nucleotide sequence of a 3.5 kb fragment containing the catalase-peroxidase gene and its flanking regions was determined. A 2.16 kb open reading frame was obtained, encoding the enzyme which was comprised of 720 amino acid residues with a calculated molecular weight of 80 kDa. The deduced amino acid sequence of the H. salinarum catalase-peroxidase showed a high degree of identity to other bifunctional catalase-peroxidases. A transcriptional start site was identified 183 bp upstream of the trans-lational start codon. Southern blot analysis indicated that catalase-peroxidase was a single copy gene. The Archaeal catalase-peroxidase gene was expressed in Escherichia coli, and the expressed fusion protein exhibited both catalase and peroxidase activities.
从古细菌(Halobacterium salinarum)的染色体DNA中克隆了编码过氧化氢酶的基因。测定了含有过氧化氢酶-过氧化物酶基因及其侧翼区域的3.5 kb片段的核苷酸序列。得到一个2.16 kb的开放阅读框,编码该酶,该酶由720个氨基酸残基组成,计算分子量为80 kDa。盐碱地过氧化氢酶的氨基酸序列与其他双功能过氧化氢酶具有高度的同源性。转录起始位点位于转录起始密码子上游183 bp处。Southern blot分析表明,过氧化氢酶-过氧化物酶为单拷贝基因。在大肠杆菌中表达了古细菌过氧化氢酶-过氧化物酶基因,表达的融合蛋白具有过氧化氢酶和过氧化物酶活性。
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引用次数: 5
Identification of a Gene Cluster for the Mevalonate Pathway in Lactobacillus Helveticus Helveticus乳杆菌甲羟戊酸途径基因簇的鉴定
Pub Date : 2001-01-01 DOI: 10.3109/10425170109080773
A. Smeds, T. Joutsjoki, A. Palva
Three Lactobacillus helveticus 53/7 genes essential for the biosynthesis of isopentenyl diphosphate and the gene coding for a putative carotenoid biosynthesis protein were for the first time identified from lactic acid bacteria. The deduced amino acid sequences of the mevalonate pathway gene products share significant identity with corresponding proteins of a few gram-positive cocci and Streptomyces species.
首次从乳酸菌中鉴定出3个helveticus乳杆菌53/7合成二磷酸异戊烯基必需基因和一个推测的类胡萝卜素生物合成蛋白的编码基因。甲羟戊酸途径基因产物的氨基酸序列与一些革兰氏阳性球菌和链霉菌的相应蛋白具有显著的一致性。
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引用次数: 5
Molecular Cloning of cDNA Encoding Polypeptide Chain Elongation Factor la from Goldfish (Carassius auratus) 金鱼多肽链延伸因子la cDNA的分子克隆
Pub Date : 2001-01-01 DOI: 10.3109/10425170109084468
M. Tokumoto, Y. Nagahama, T. Tokumoto
The goldfish homologue of polypeptide chain elongation factor-la (EF-la) isolated from the ovary of the goldfish is described. The deduced amino acid sequence is highly homologous to EF-la from other species. Analysis of its tissue distribution revealed a single 1.7 kilobase message ubiquitous among various tissues.
描述了从金鱼卵巢中分离到的多肽链延伸因子-la (EF-la)的金鱼同源物。推导出的氨基酸序列与其他物种的EF-la高度同源。对其组织分布的分析显示,在各种组织中普遍存在一个1.7千碱基的信息。
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引用次数: 4
Genomic DNA Sequence and Transcription Factor Binding Sites of Mouse Ninjurin 小鼠忍尿蛋白基因组DNA序列及转录因子结合位点
Pub Date : 2001-01-01 DOI: 10.3109/10425170109084463
Ae Ran Moon, G. Oh, Jae Wha Kim, Y. Cho, I. Choe
The complete genomic DNA sequence of mouse ninjurin gene has been cloned and sequenced by screening a bacterial artificial chromosome (BAC) library of mouse 129/SvJ genomic DNA. The mouse ninjurin gene comprises four exons and the translatable sequences are included in the first three exons. The putative promoter region of the mouse ninjurin gene lacks the consensus “CAAT” or “TATA” sequence. Nonetheless, it has demonstrated the promoter activity in transient transfection experiment using the construct containing putative promoter sequence of mouse ninjurin and reporter gene. The nucleotide sequence of the putative promoter region shows 83% homology with the corresponding DNA sequence of human ninjurin gene that had been previously reported, and reveals a high degree of conservation between the two species. Analysis of the DNA sequence identified the putative promoters and the binding sites for a variety of transcription factors of mouse ninjurin.
通过筛选细菌人工染色体(BAC)小鼠129/SvJ基因组DNA文库,克隆并测序了小鼠忍尿素基因的全基因组DNA序列。小鼠ninjurin基因包括4个外显子,可翻译序列包含在前3个外显子中。小鼠ninjurin基因的推定启动子区域缺乏共识的“CAAT”或“TATA”序列。然而,利用含有小鼠忍者蛋白和报告基因启动子序列的构建体,在瞬时转染实验中证明了启动子的活性。该启动子区域的核苷酸序列与已有报道的人类忍者基因的DNA序列同源性为83%,显示了两者之间的高度保守性。DNA序列分析确定了小鼠忍者蛋白的启动子和多种转录因子的结合位点。
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引用次数: 4
期刊
DNA Sequence
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