Advanced Healthcare Materials最新文献

Although the idea of employing cell membrane biomimetic technologies for detecting circulating tumor cells (CTCs) promises paradigm-shifting advances in cancer diagnostics, its wide application is restricted by CTCs phenotypic variations. Current improvements primarily focus on optimizing biomimetic coatings to enhance capture efficiency, but there remains a significant gap between existing strategies and the practical demands of CTCs detection. Herein, a novel method considering the perspective of tumor cell modification was proposed, which involved concurrently modulating cellular chemical and mechanical properties. Specifically, the strategy employed metabolic glycoengineering to selectively remold tumor cells, thereby introducing artificial receptors into the tumor cell membrane. Additionally, Cytochalasin D, a drug that can interfere with the cytoskeleton, was used to alter the mechanical properties of the cell membrane, softening it and thereby significantly enhancing the contact area and adhesion ability between target cells and the substrate surface. To cope with the complex application environment, a visual biomimetic detection system was developed, leveraging the homologous targeting properties of the tumor cell biomimetic layer in combination with advanced colorimetric nanoprobes, enabling highly sensitive and specific detection of engineered CTCs. Overall, this approach adeptly circumvents challenges associated with biomarker bias, offering a robust method for non-invasive cancer diagnostics.

Infections caused by bacterial colonization and biofilm formation on wounds and dressings present critical challenges to wound care, often impeding healing. Here, we report an antibiotic-free preventive strategy based on medical fabric coated with supramolecular antimicrobial assemblies. Using layer-by-layer dip coating technique, we functionalized medical fabric with polyarginine (PAR30) and hyaluronic acid (HA144) polymers, biopolymers that synergistically exhibited intrinsic antimicrobial activity. Coatings deposition and structural integrity were validated by confocal microscopy and ATR-FTIR spectroscopy. Antibacterial performance was assessed using the AATCC100 standard test method, showed strong efficacy against both Gram-negative and Gram-positive clinical pathogens. In vivo wound infection models, employing bioluminescent methicillin-resistant Staphylococcus aureus (MRSA), were used to evaluate biofilm prevention. Coated and uncoated fabrics were either pre-inoculated with MRSA or applied to pre-infected wounds to assess their antimicrobial and anti-biofilm effects. The coated fabrics showed potent antibacterial activity, achieving ≥6 log-reduction in bacterial load within 24 h compared to uncoated fabrics. Bioluminescence imaging confirmed infection development in wounds covered with uncoated fabrics, while coated fabrics prevented infection with a ≥6 log-reduction in bacterial load on fabrics and a ≥4 log-reduction in wound biopsies. Additionally, coated fabrics inhibited biofilm formation and bacterial proliferation in wound beds inoculated with MRSA. Comprehensive in vitro and in vivo biocompatibility assessments demonstrated the safe profile of the coated fabrics for clinical use. These findings highlight the antimicrobial efficiency of coated fabrics in minimizing bacterial colonization and biofilm formation on wounds and textiles. This safe and effective first-in-class, innovative approach offers a promising preventive strategy against biofilm formation and addresses antimicrobial-resistant strains like MRSA in wound care.

Immune Modulation for Osteogenesis

In the Research Article (DOI: 10.1002/adhm.202502466), J. Kent Leach and co-workers show that inflammatory microenvironments are influenced by controlled release of immunomodulatory factors. The designed effects are two-fold: to alter macrophage polarization to an anti-inflammatory phenotype and promote mesenchymal stromal cell differentiation to osteoblasts.

Glioblastoma-on-a-Chip for Nanomedicine

This cover highlights a tumor-on-a-chip model that mimics the glioblastoma perivascular niche. The platform is used to test monocyte membrane-coated nanoparticles (MoNP), which camouflage as monocytes to improve vascular targeting. By exploiting tumor-activated blood vessels, MoNP enhance drug delivery, offering a powerful tool to explore therapeutic strategies in a realistic 3D tumor environment. More details can be found in the Research Article by Kuei-Chun Wang, Mehdi Nikkhah, and co-workers (DOI: 10.1002/adhm.202502454).

Wound Dressing

This work transforms chitosan, a natural polymer derived from crustacean shells, into a smart, multifunctional wound dressing. Through integration with complementary polymers and silver nanoparticles, the material accelerates healing while enhancing protection and adaptability. More details can be found in the Research Article by Chun Che Lin and co-workers (DOI: 10.1002/adhm.202502971).

3D Bioprinting

This cover depicts a 3D bioprinted, patient-specific carotid artery model integrating anatomical geometry, hemodynamic validation, and vascular cell response. Combining computational flow simulations, biomimetic perfusion, and human vascular cells, it reveals how local variation in shear stress drives different endothelial remodeling and monocyte adhesion, providing a high-fidelity platform to study atherosclerosis progression. More details can be found in the Research Article by Yi-Chin Toh, Zhiyong Li, and co-workers (DOI: 10.1002/adhm.202502478).

Polypropylene (PP) mesh implanting has emerged as a breast reconstruction approach to restore normal anatomical contours. However, developing biomedical PP mesh with enhanced anti-inflammatory properties, superior mechanical strength, and efficient manufacturing remains a significant challenge. In this study, we report a drug-loading PP mesh by incorporating triclosan into femtosecond (fs) laser-treated PP mesh scaffolds. The fs laser induced micro/nano structures significantly enhanced the drug-loading capacity and antibacterial efficacy of triclosan-modified PP mesh, as indicated by the area of inhibition rings (2.5-fold relative to the blank groups). In vivo breast reconstruction experiments using female Sprague-Dawley (SD) rats demonstrate accelerated scab formation and wound healing. Additionally, the Enzyme-linked immunosorbent assay (ELISA) test reveals high vascular density (11.43 µg/mL of CD31 and 0.32 µg/mL of CD163) and reduced proinflammatory cytokine levels (145 pg/mL of IL-6 and 33 pg/mL of TNF-a), further confirming the anti-inflammatory properties of the fs laser-treated triclosan-modified PP mesh. This study presents a novel approach to improving the clinical performance of PP meshes for biomedical applications.

The development of assembly interfaces that emulate subcellular chemical communication at the biotic-abiotic interface is a central pursuit in synthetic biology. However, engineering in situ self-assembling systems that combine robust retention against mucosal flushing and microenvironmental regulatory functions remains a formidable challenge. Herein, we present an intravesical in situ synthetic strategy that exploits endogenous microenvironmental triggers to construct a glycosaminoglycan (GAG)-mimetic coating for durable mucosal repair. By leveraging catalase (CAT) and hydrogen peroxide (H₂O₂) inherent to inflammatory microenvironments, we drive the polymerization of alginate oligosaccharide-conjugated polydopamine (AOS-PDA). The resulting coating recapitulates the anionic and barrier properties while exhibiting anti-inflammatory activity. In rat models of cyclophosphamide (CYP)-induced interstitial cystitis, AOS-PDA treatment significantly restored urothelial barrier integrity, reduced vascular permeability, and alleviated bladder dysfunction. Histological and molecular analyses confirmed the attenuation of inflammation, characterized by decreased mast cell infiltration and downregulation of key inflammatory mediators. Furthermore, the coating demonstrated sustained retention for up to three days post-administration. These findings highlight a bio-responsive, endogenous enzyme-driven approach to synthesizing functional GAG-like interfaces, offering a versatile therapeutic platform for broader mucosal repair applications.

While current treatments for multiple sclerosis (MS) targeting T or B cells have shown clinical benefit, they remain insufficient due to the multifactorial and dynamic nature of MS pathogenesis. A key obstacle to restoring immune homeostasis lies in the reciprocal reinforcement between endothelial-to-mesenchymal transition (EndMT) and sustained immune infiltration, which collectively exacerbate blood-brain barrier (BBB) disruption and neuroinflammation. We identified IL7R as a shared target on endothelial cells and pathogenic T cells via scRNA-seq and developed a multifunctional nanodelivery platform-ETS1 pDNA/PBAE@ITP-MM (IMNP)-comprising ETS1 plasmid DNA complexed with poly(β-amino ester) (PBAE), an interleukin-7 receptor (IL7R)-targeting peptide (ITP), and a macrophage membrane (MM) coating. IMNP concurrently modulates endothelial cells and T lymphocytes for synergistic efficacy. Exploiting the intrinsic inflammation-targeting capacity of activated macrophage membranes and ITP conjugation, IMNPs preferentially accumulate at the vascular endothelium, where they inhibit EndMT and preserve BBB integrity. Subsequently, IMNPs attenuate differentiation of CD4⁺ T cells into proinflammatory Th1/Th17 subsets, thereby reducing CNS infiltration and re-establishing immune microenvironmental balance in both relapsing-remitting MS (RRMS) and secondary progressive MS (SPMS) models. Our findings provide proof-of-concept for a biomimetic nanoplatform that achieves dual vascular-immune modulation, significantly alleviating neuroinflammation, reducing demyelination, and improving motor performance in both RRMS and SPMS models.