In article 2400538, Yingqi Hua, Xuran Guo, Jing Xu, Zhengdong Cai, and co-workers design a new multicombination strategy that reprograms the immunosuppressive tumor microenvironment and enhances the effect of photodynamic therapy for osteosarcoma treatment. The combination of A-NPs@(SHK+Ce6) with programmed cell death protein 1 demonstrates remarkable ability in eliminating distant tumors and promoting long-term immune memory function, thereby protecting against tumor rechallenge.
骨肉瘤光免疫疗法
{"title":"Enhanced Osteosarcoma Immunotherapy via CaCO3 Nanoparticles: Remodeling Tumor Acidic and Immune Microenvironment for Photodynamic Therapy (Adv. Healthcare Mater. 23/2024)","authors":"Yinghua Gao, Zhuoying Wang, Xinmeng Jin, Xiaoli Wang, Yining Tao, Shandeng Huang, Yun Wang, Yingqi Hua, Xuran Guo, Jing Xu, Zhengdong Cai","doi":"10.1002/adhm.202470147","DOIUrl":"10.1002/adhm.202470147","url":null,"abstract":"<p><b>Osteosarcoma Photoimmunotherapy</b></p><p>In article 2400538, Yingqi Hua, Xuran Guo, Jing Xu, Zhengdong Cai, and co-workers design a new multicombination strategy that reprograms the immunosuppressive tumor microenvironment and enhances the effect of photodynamic therapy for osteosarcoma treatment. The combination of A-NPs@(SHK+Ce6) with programmed cell death protein 1 demonstrates remarkable ability in eliminating distant tumors and promoting long-term immune memory function, thereby protecting against tumor rechallenge.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.202470147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wang Xin, Shuiqin Gong, Yin Chen, Mengying Yao, Shaozong Qin, Jing Chen, Aihong Zhang, Wenrui Yu, Siyan Zhou, Bo Zhang, Jun Gu, Jinghong Zhao, Yinghui Huang
Self-Assembling Inhibitory Peptide Nanoparticles
In article 2400441, Jun Gu, Jinghong Zhao, Yinghui Huang, and co-workers devise a self-assembling p38 inhibitory peptide nanoparticle (TMNP), which exhibits renal targeting, ROS-scavenging, and ferroptosis-mitigating abilities, making it a promising therapeutic agent for the treatment of acute kidney injury (AKI) and its progression to chronic kidney disease (CKD).
自组装抑制肽纳米粒子
{"title":"Self-Assembling P38 Peptide Inhibitor Nanoparticles Ameliorate the Transition from Acute to Chronic Kidney Disease by Suppressing Ferroptosis (Adv. Healthcare Mater. 23/2024)","authors":"Wang Xin, Shuiqin Gong, Yin Chen, Mengying Yao, Shaozong Qin, Jing Chen, Aihong Zhang, Wenrui Yu, Siyan Zhou, Bo Zhang, Jun Gu, Jinghong Zhao, Yinghui Huang","doi":"10.1002/adhm.202470149","DOIUrl":"10.1002/adhm.202470149","url":null,"abstract":"<p><b>Self-Assembling Inhibitory Peptide Nanoparticles</b></p><p>In article 2400441, Jun Gu, Jinghong Zhao, Yinghui Huang, and co-workers devise a self-assembling p38 inhibitory peptide nanoparticle (TMNP), which exhibits renal targeting, ROS-scavenging, and ferroptosis-mitigating abilities, making it a promising therapeutic agent for the treatment of acute kidney injury (AKI) and its progression to chronic kidney disease (CKD).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adhm.202470149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antigen delivery via respiratory mucosal surfaces is an interesting needle‐free option for vaccination. Nonetheless, it demands for the design of especially tailored formulations. Here, lipid/poly(lactic‐co‐glycolic) acid (PLGA) hybrid nanoparticles (hNPs) for the combined delivery of an antigen, ovalbumin (Ova), and an adjuvant, synthetic unmethylated cytosine‐phosphate‐guanine oligodeoxynucleotide (CpG) motifs, is developed. A panel of Ova/CpG‐loaded lipid@PLGA hNPs with tunable size and surface is attained by exploiting two lipid moieties, 1,2 distearoil‐sn‐glycero‐3‐phosphoethanolamine‐poly(ethylene glycol) (DSPE‐PEG) and monophosphoryl lipid A (MPLA), with or without polyethyleneimine (PEI). It is gained insights on the lipid@PLGA hNPs through a combination of techniques to analytically determine the specific moiety on the surface, the spatial distribution of the components and the internal structure of the nanoplatforms. The collected results suggest that PEI plays a role of paramount importance not only in promoting in vitro antigen escape from lysosomes and enhancing antigen cross‐presentation, but also in determining the arrangement of the moieties in the final architecture of the hNPs. Though multicomponent PEI‐engineered lipid@PLGA hNPs turn out as a viable strategy for delivery of antigens and adjuvant to the respiratory mucosa, tunable nanoparticle features are achievable only through the optimal selection of the components and their relative amounts.
{"title":"PEI‐Engineered Lipid@PLGA Hybrid Nanoparticles for Multimodal Delivery of Antigens and Immune Adjuvants to the Respiratory Mucosa","authors":"Susy Brusco, Gemma Conte, Annunziata Corteggio, Teresa Silvestri, Andrea Spitaleri, Paola Brocca, Agnese Miro, Fabiana Quaglia, Ivana d'Angelo, Luciana D'Apice, Paola Italiani, Gabriella Costabile, Francesca Ungaro","doi":"10.1002/adhm.202402688","DOIUrl":"https://doi.org/10.1002/adhm.202402688","url":null,"abstract":"Antigen delivery via respiratory mucosal surfaces is an interesting needle‐free option for vaccination. Nonetheless, it demands for the design of especially tailored formulations. Here, lipid/poly(lactic‐co‐glycolic) acid (PLGA) hybrid nanoparticles (hNPs) for the combined delivery of an antigen, ovalbumin (Ova), and an adjuvant, synthetic unmethylated cytosine‐phosphate‐guanine oligodeoxynucleotide (CpG) motifs, is developed. A panel of Ova/CpG‐loaded lipid@PLGA hNPs with tunable size and surface is attained by exploiting two lipid moieties, 1,2 distearoil‐sn‐glycero‐3‐phosphoethanolamine‐poly(ethylene glycol) (DSPE‐PEG) and monophosphoryl lipid A (MPLA), with or without polyethyleneimine (PEI). It is gained insights on the lipid@PLGA hNPs through a combination of techniques to analytically determine the specific moiety on the surface, the spatial distribution of the components and the internal structure of the nanoplatforms. The collected results suggest that PEI plays a role of paramount importance not only in promoting in vitro antigen escape from lysosomes and enhancing antigen cross‐presentation, but also in determining the arrangement of the moieties in the final architecture of the hNPs. Though multicomponent PEI‐engineered lipid@PLGA hNPs turn out as a viable strategy for delivery of antigens and adjuvant to the respiratory mucosa, tunable nanoparticle features are achievable only through the optimal selection of the components and their relative amounts.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eseelle K. Hendow, Francesco Iacoviello, Mar Casajuana Ester, Caroline Pellet‐Many, Richard M. Day
Promoting neovascularization is a prerequisite for many tissue engineering applications and the treatment of cardiovascular disease. Delivery of a pro‐angiogenic stimulus via acellular materials offers several benefits over biological therapies but has been hampered by interaction of the implanted material with the innate immune response. However, macrophages, a key component of the innate immune response, release a plurality of soluble factors that can be harnessed to stimulate neovascularization and restore blood flow to damaged tissue. This study investigates the ability of biodegradable poly(D,L‐lactic‐co‐glycolic acid) (PLGA) microspheres to restore tissue perfusion in a hind limb model of ischaemia. Microspheres exhibiting a hierarchical porous structure are associated with an increase in blood flow at day 21 post‐implantation compared with solid microspheres composed of the same polymer. This corresponds with an increase in blood vessel density in the surrounding tissue. In vitro simulation of the foreign body response observed demonstrates M2‐like macrophages incubated with the porous microspheres secreted increased amounts of vascular endothelial growth factor (VEGF) compared with M1‐like macrophages providing a potential mechanism for the increased neovascularization. The results from this study demonstrate implantable biodegradable porous microspheres provide a novel approach for increasing neovascularization that could be exploited for therapeutic applications.
{"title":"Hierarchically Structured Biodegradable Microspheres Promote Therapeutic Angiogenesis","authors":"Eseelle K. Hendow, Francesco Iacoviello, Mar Casajuana Ester, Caroline Pellet‐Many, Richard M. Day","doi":"10.1002/adhm.202401832","DOIUrl":"https://doi.org/10.1002/adhm.202401832","url":null,"abstract":"Promoting neovascularization is a prerequisite for many tissue engineering applications and the treatment of cardiovascular disease. Delivery of a pro‐angiogenic stimulus via acellular materials offers several benefits over biological therapies but has been hampered by interaction of the implanted material with the innate immune response. However, macrophages, a key component of the innate immune response, release a plurality of soluble factors that can be harnessed to stimulate neovascularization and restore blood flow to damaged tissue. This study investigates the ability of biodegradable poly(D,L‐lactic‐co‐glycolic acid) (PLGA) microspheres to restore tissue perfusion in a hind limb model of ischaemia. Microspheres exhibiting a hierarchical porous structure are associated with an increase in blood flow at day 21 post‐implantation compared with solid microspheres composed of the same polymer. This corresponds with an increase in blood vessel density in the surrounding tissue. In vitro simulation of the foreign body response observed demonstrates M2‐like macrophages incubated with the porous microspheres secreted increased amounts of vascular endothelial growth factor (VEGF) compared with M1‐like macrophages providing a potential mechanism for the increased neovascularization. The results from this study demonstrate implantable biodegradable porous microspheres provide a novel approach for increasing neovascularization that could be exploited for therapeutic applications.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142227611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Delong Xu, Shenghui Bi, Jiejing Li, Shaodan Ma, Ze‐an Yu, Yenan Wang, Huiming Chen, Jie Zhan, Xudong Song, Yanbin Cai
Promoting angiogenesis and modulating the inflammatory microenvironment are promising strategies for treating acute myocardial infarction (MI). Macrophages are crucial in regulating inflammation and influencing angiogenesis through interactions with endothelial cells. However, current therapies lack a comprehensive assessment of pathological and physiological subtleties, resulting in limited myocardial recovery. In this study, legumain‐guided ferulate‐peptide nanofibers (LFPN) are developed to facilitate the interaction between macrophages and endothelial cells in the MI lesion and modulate their functions. LFPN exhibits enhanced ferulic acid (FA) aggregation and release, promoting angiogenesis and alleviating inflammation. The multifunctional role of LFPN is validated in cells and an MI mouse model, where it modulated macrophage polarization, attenuated inflammatory responses, and induces endothelial cell neovascularization compare to FA alone. LFPN supports the preservation of border zone cardiomyocytes by regulating inflammatory infiltration in the ischemic core, leading to significant functional recovery of the left ventricle. These findings suggest that synergistic therapy exploiting multicellular interaction and enzyme guidance may enhance the clinical translation potential of smart‐responsive drug delivery systems to treat MI. This work emphasizes macrophage‐endothelial cell partnerships as a novel paradigm to enhance cell interactions, control inflammation, and promote therapeutic angiogenesis.
{"title":"Legumain‐Guided Ferulate‐Peptide Self‐Assembly Enhances Macrophage‐Endotheliocyte Partnership to Promote Therapeutic Angiogenesis After Myocardial Infarction","authors":"Delong Xu, Shenghui Bi, Jiejing Li, Shaodan Ma, Ze‐an Yu, Yenan Wang, Huiming Chen, Jie Zhan, Xudong Song, Yanbin Cai","doi":"10.1002/adhm.202402056","DOIUrl":"https://doi.org/10.1002/adhm.202402056","url":null,"abstract":"Promoting angiogenesis and modulating the inflammatory microenvironment are promising strategies for treating acute myocardial infarction (MI). Macrophages are crucial in regulating inflammation and influencing angiogenesis through interactions with endothelial cells. However, current therapies lack a comprehensive assessment of pathological and physiological subtleties, resulting in limited myocardial recovery. In this study, legumain‐guided ferulate‐peptide nanofibers (LFPN) are developed to facilitate the interaction between macrophages and endothelial cells in the MI lesion and modulate their functions. LFPN exhibits enhanced ferulic acid (FA) aggregation and release, promoting angiogenesis and alleviating inflammation. The multifunctional role of LFPN is validated in cells and an MI mouse model, where it modulated macrophage polarization, attenuated inflammatory responses, and induces endothelial cell neovascularization compare to FA alone. LFPN supports the preservation of border zone cardiomyocytes by regulating inflammatory infiltration in the ischemic core, leading to significant functional recovery of the left ventricle. These findings suggest that synergistic therapy exploiting multicellular interaction and enzyme guidance may enhance the clinical translation potential of smart‐responsive drug delivery systems to treat MI. This work emphasizes macrophage‐endothelial cell partnerships as a novel paradigm to enhance cell interactions, control inflammation, and promote therapeutic angiogenesis.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142227608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Limei Zhang, Hao Bai, Jie Zou, Chuyan Zhang, Weihua Zhuang, Jie Hu, Yongchao Yao, Wenchuang (Walter) Hu
In the rapidly evolving field of life sciences and biomedicine, detecting low‐abundance biomolecules, and ultraweak biosignals presents significant challenges. This has spurred a rapid development of analytical techniques aiming for increased sensitivity and specificity. These advancements, including signal amplification strategies and the integration of biorecognition events, mark a transformative era in bioanalytical precision and accuracy. A prominent method among these innovations is immuno‐rolling circle amplification (immuno‐RCA) technology, which effectively combines immunoassays with signal amplification via RCA. This process starts when a targeted biomolecule, such as a protein or cell, binds to an immobilized antibody or probe on a substrate. The introduction of a circular DNA template triggers RCA, leading to exponential amplification and significantly enhanced signal intensity, thus the target molecule is detectable and quantifiable even at the single‐molecule level. This review provides an overview of the biosensing strategy and extensive practical applications of immuno‐RCA in detecting biomarkers. Furthermore, it scrutinizes the limitations inherent to these sensors and sets forth expectations for their future trajectory. This review serves as a valuable reference for advancing immuno‐RCA in various domains, such as diagnostics, biomarker discovery, and molecular imaging.
在快速发展的生命科学和生物医学领域，检测低丰度生物分子和超弱生物信号是一项重大挑战。这推动了旨在提高灵敏度和特异性的分析技术的快速发展。这些进步，包括信号放大策略和生物识别事件的整合，标志着生物分析精度和准确性进入了一个变革时代。在这些创新中，免疫滚圆放大（immuno-RCA）技术是一种突出的方法，它通过 RCA 将免疫测定与信号放大有效地结合在一起。当蛋白质或细胞等目标生物分子与底物上的固定抗体或探针结合时，这一过程就开始了。环状 DNA 模板的引入会触发 RCA，从而导致指数级放大并显著增强信号强度，因此即使在单分子水平上也能检测和量化目标分子。本综述概述了生物传感策略以及免疫 RCA 在检测生物标记物方面的广泛实际应用。此外，它还仔细分析了这些传感器固有的局限性，并对其未来的发展轨迹提出了期望。这篇综述对推动免疫 RCA 在诊断、生物标记物发现和分子成像等各个领域的应用具有重要参考价值。
{"title":"Immuno‐Rolling Circle Amplification (Immuno‐RCA): Biosensing Strategies, Practical Applications, and Future Perspectives","authors":"Limei Zhang, Hao Bai, Jie Zou, Chuyan Zhang, Weihua Zhuang, Jie Hu, Yongchao Yao, Wenchuang (Walter) Hu","doi":"10.1002/adhm.202402337","DOIUrl":"https://doi.org/10.1002/adhm.202402337","url":null,"abstract":"In the rapidly evolving field of life sciences and biomedicine, detecting low‐abundance biomolecules, and ultraweak biosignals presents significant challenges. This has spurred a rapid development of analytical techniques aiming for increased sensitivity and specificity. These advancements, including signal amplification strategies and the integration of biorecognition events, mark a transformative era in bioanalytical precision and accuracy. A prominent method among these innovations is immuno‐rolling circle amplification (immuno‐RCA) technology, which effectively combines immunoassays with signal amplification via RCA. This process starts when a targeted biomolecule, such as a protein or cell, binds to an immobilized antibody or probe on a substrate. The introduction of a circular DNA template triggers RCA, leading to exponential amplification and significantly enhanced signal intensity, thus the target molecule is detectable and quantifiable even at the single‐molecule level. This review provides an overview of the biosensing strategy and extensive practical applications of immuno‐RCA in detecting biomarkers. Furthermore, it scrutinizes the limitations inherent to these sensors and sets forth expectations for their future trajectory. This review serves as a valuable reference for advancing immuno‐RCA in various domains, such as diagnostics, biomarker discovery, and molecular imaging.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Li Zhang, Dongze Li, Yierpani Aierken, Jie Zhang, Zhenyu Liu, Zipeng Lin, Longqi Jiang, Qingzhu Li, Ya Wu, Yong Liu
Cardiovascular disease (CVD) is a leading cause of death globally, and vascular calcification (VC) is an important independent risk factor for predicting CVD. Currently, there are no established therapeutic strategies for the treatment of VC. Although recognized combination therapies of nanomedicines can provide effective strategies for disease treatment, the clinical application of nanomedicines is limited because of their complex preparation processes, low drug loading rates, and unpredictable safety risks. Thus, developing a simple, efficient, and safe nanodrug to simultaneously regulate inflammation and autophagy may be a promising strategy for treating VC. Herein, an anti‐inflammatory peptide (lysine‐proline‐valine peptides, KPV) and the autophagy activator rapamycin (RAPA) are self‐assembled to form new carrier‐free spherical nanoparticles (NPs), which shows good stability and biosafety. In vivo and in vitro, KPV‐RAPA NPs significantly inhibit VC in mice compared to the other treatment groups. Mechanistically, KPV‐RAPA NPs inhibit inflammatory responses and activated autophagy. Therefore, this study indicates that the new carrier‐free KPV‐RAPA NPs have great potential as therapeutic agents for VC combination therapy, which can promote the development of nanodrugs for VC.
{"title":"KPV and RAPA Self‐Assembled into Carrier‐Free Nanodrugs for Vascular Calcification Therapy","authors":"Li Zhang, Dongze Li, Yierpani Aierken, Jie Zhang, Zhenyu Liu, Zipeng Lin, Longqi Jiang, Qingzhu Li, Ya Wu, Yong Liu","doi":"10.1002/adhm.202402320","DOIUrl":"https://doi.org/10.1002/adhm.202402320","url":null,"abstract":"Cardiovascular disease (CVD) is a leading cause of death globally, and vascular calcification (VC) is an important independent risk factor for predicting CVD. Currently, there are no established therapeutic strategies for the treatment of VC. Although recognized combination therapies of nanomedicines can provide effective strategies for disease treatment, the clinical application of nanomedicines is limited because of their complex preparation processes, low drug loading rates, and unpredictable safety risks. Thus, developing a simple, efficient, and safe nanodrug to simultaneously regulate inflammation and autophagy may be a promising strategy for treating VC. Herein, an anti‐inflammatory peptide (lysine‐proline‐valine peptides, KPV) and the autophagy activator rapamycin (RAPA) are self‐assembled to form new carrier‐free spherical nanoparticles (NPs), which shows good stability and biosafety. In vivo and in vitro, KPV‐RAPA NPs significantly inhibit VC in mice compared to the other treatment groups. Mechanistically, KPV‐RAPA NPs inhibit inflammatory responses and activated autophagy. Therefore, this study indicates that the new carrier‐free KPV‐RAPA NPs have great potential as therapeutic agents for VC combination therapy, which can promote the development of nanodrugs for VC.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yujian Hui, Jiannan Mao, Min Rui, Yiyang Huang, Xinzhao Jiang, Yichang Xu, Wei Wang, Jie Wu, Liang Zhou, Kun Xi, Lixin Huang, Liang Chen
The healing of bone defects among diabetic patients presents a critical challenge due to the pathological microenvironment, characterized by hyperglycemia, excessive reactive oxygen species (ROS) production, and inflammation. Herein, multifunctional composite microspheres, termed GMAP are developed, using a microfluidic technique by incorporating Au@Pt nanoparticles (NPs) and GelMA hydrogel to modulate the diabetic microenvironment for promoting bone regeneration. The GMAP enables the sustained release of Au@Pt NPs, which function as bimetallic nanozymes with dual enzyme‐like activities involving glucose oxidase and catalase. The synergistic effect allows for efficient glucose consumption and ROS elimination concurrently. Thus, the GMAP effectively protects the proliferation of bone marrow mesenchymal stem cells (BMSCs) under adverse high‐glucose conditions. Furthermore, it also promotes the osteogenic differentiation and paracrine capabilities of BMSCs, and subsequently inhibits inflammation and enhances angiogenesis. In vivo diabetic rats bone defect model, it is demonstrated that GMAP microspheres significantly improve bone regeneration, as verified by micro‐computed tomography and histological examinations. This study provides a novel strategy for bone regeneration by modulating the diabetic microenvironment, presenting a promising approach for addressing the complex challenges associated with bone healing in diabetic patients.
{"title":"Hydrogel Microsphere‐Encapsulated Bimetallic Nanozyme for Promoting Diabetic Bone Regeneration via Glucose Consumption and ROS Scavenging","authors":"Yujian Hui, Jiannan Mao, Min Rui, Yiyang Huang, Xinzhao Jiang, Yichang Xu, Wei Wang, Jie Wu, Liang Zhou, Kun Xi, Lixin Huang, Liang Chen","doi":"10.1002/adhm.202402596","DOIUrl":"https://doi.org/10.1002/adhm.202402596","url":null,"abstract":"The healing of bone defects among diabetic patients presents a critical challenge due to the pathological microenvironment, characterized by hyperglycemia, excessive reactive oxygen species (ROS) production, and inflammation. Herein, multifunctional composite microspheres, termed GMAP are developed, using a microfluidic technique by incorporating Au@Pt nanoparticles (NPs) and GelMA hydrogel to modulate the diabetic microenvironment for promoting bone regeneration. The GMAP enables the sustained release of Au@Pt NPs, which function as bimetallic nanozymes with dual enzyme‐like activities involving glucose oxidase and catalase. The synergistic effect allows for efficient glucose consumption and ROS elimination concurrently. Thus, the GMAP effectively protects the proliferation of bone marrow mesenchymal stem cells (BMSCs) under adverse high‐glucose conditions. Furthermore, it also promotes the osteogenic differentiation and paracrine capabilities of BMSCs, and subsequently inhibits inflammation and enhances angiogenesis. In vivo diabetic rats bone defect model, it is demonstrated that GMAP microspheres significantly improve bone regeneration, as verified by micro‐computed tomography and histological examinations. This study provides a novel strategy for bone regeneration by modulating the diabetic microenvironment, presenting a promising approach for addressing the complex challenges associated with bone healing in diabetic patients.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Julia Simińska‐Stanny, Daria Podstawczyk, Christine Delporte, Lei Nie, Armin Shavandi
Tissue vascularization is a major bottleneck in tissue engineering. In this review, the state of the art on the intricate role of hyaluronic acid (HA) in angiogenesis is explored. HA plays a twofold role in angiogenesis. First, when released as a free polymer in the extracellular matrix (ECM), HA acts as a signaling molecule triggering multiple cascades that foster smooth muscle cell differentiation, migration, and proliferation thereby contributing to vessel wall thickening. Simultaneously, HA bound to the plasma membrane in the pericellular space functions as a polymer block, participating in vessel formation. Starting with the HA origins in native vascular tissues, the approaches aimed at achieving vascularization in vivo are reviewed. The significance of HA molecular weight (MW) in angiogenesis and the challenges associated with utilizing HA in vascular tissue engineering (VTE) are conscientiously addressed. The review finally focuses on a thorough examination and comparison of the diverse strategies adopted to harness the benefits of HA in the vascularization of bioengineered materials. By providing a nuanced perspective on the multifaceted role of HA in angiogenesis, this review contributes to the ongoing discourse in tissue engineering and advances the collective understanding of optimizing vascularization processes assisted by functional biomaterials.
组织血管化是组织工程中的一大瓶颈。本综述探讨了透明质酸（HA）在血管生成中的复杂作用。透明质酸在血管生成中起着双重作用。首先，当透明质酸作为游离聚合物释放到细胞外基质（ECM）中时，它是一种信号分子，可触发多种级联反应，促进平滑肌细胞分化、迁移和增殖，从而促使血管壁增厚。同时，在细胞周围空间与质膜结合的 HA 可作为聚合物块，参与血管的形成。本文从 HA 在原生血管组织中的起源入手，回顾了旨在实现体内血管化的方法。文章认真探讨了 HA 分子重量（MW）在血管生成中的意义，以及在血管组织工程（VTE）中利用 HA 所面临的挑战。最后，本综述重点对利用 HA 在生物工程材料血管化中的益处所采用的各种策略进行了深入研究和比较。通过对 HA 在血管生成中的多方面作用提供细致入微的透视，这篇综述为组织工程领域正在进行的讨论做出了贡献，并推动了对功能性生物材料辅助血管生成过程优化的集体理解。
{"title":"Hyaluronic Acid Role in Biomaterials Prevascularization","authors":"Julia Simińska‐Stanny, Daria Podstawczyk, Christine Delporte, Lei Nie, Armin Shavandi","doi":"10.1002/adhm.202402045","DOIUrl":"https://doi.org/10.1002/adhm.202402045","url":null,"abstract":"Tissue vascularization is a major bottleneck in tissue engineering. In this review, the state of the art on the intricate role of hyaluronic acid (HA) in angiogenesis is explored. HA plays a twofold role in angiogenesis. First, when released as a free polymer in the extracellular matrix (ECM), HA acts as a signaling molecule triggering multiple cascades that foster smooth muscle cell differentiation, migration, and proliferation thereby contributing to vessel wall thickening. Simultaneously, HA bound to the plasma membrane in the pericellular space functions as a polymer block, participating in vessel formation. Starting with the HA origins in native vascular tissues, the approaches aimed at achieving vascularization in vivo are reviewed. The significance of HA molecular weight (MW) in angiogenesis and the challenges associated with utilizing HA in vascular tissue engineering (VTE) are conscientiously addressed. The review finally focuses on a thorough examination and comparison of the diverse strategies adopted to harness the benefits of HA in the vascularization of bioengineered materials. By providing a nuanced perspective on the multifaceted role of HA in angiogenesis, this review contributes to the ongoing discourse in tissue engineering and advances the collective understanding of optimizing vascularization processes assisted by functional biomaterials.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Minzi Ju, Zhongkun Zhang, Feng Gao, Gang Chen, Sibo Zhao, Dan Wang, Huijuan Wang, Yanpeng Jia, Ling Shen, Yonggui Yuan, Honghong Yao
Major depressive disorder (MDD) is a prevalent mental disorder that significantly impacts social and psychological function, but no effective medication is currently available. Circular RNAs (circRNAs) have been reported to participate in the pathogenesis of MDD which are envisioned as promising therapeutic targets. However, nonviral‐based delivery strategies targeting circRNA against MDD are not thoroughly investigated. Here, it is identified that circATF7IP is significantly upregulated in plasma samples and positively correlated with 24‐Hamilton Depression Scale (HAMD‐24) scores of MDD patients. Synergistic amine lipid nanoparticles (SALNPs) are designed to deliver siRNA targeting circATF7IP (si‐circATF7IP) into the hippocampus brain region by intranasal administration. Intranasal delivery of SALNP‐si‐circATF7IP successfully alleviated the depressive‐like behaviors in the LPS‐induced mouse depression model via decreasing CD11b+CD45dim microglia population and pro‐inflammatory cytokine productions (TNF‐α and IL‐6). These results indicate that the level of circATF7IP positively correlates with MDD pathogenesis, and SALNP delivery of si‐circATF7IP via intranasal administration is an effective strategy to ameliorate LPS‐induced depressive‐like behaviors.
{"title":"Intranasal Delivery of circATF7IP siRNA via Lipid Nanoparticles Alleviates LPS‐induced Depressive‐Like Behaviors","authors":"Minzi Ju, Zhongkun Zhang, Feng Gao, Gang Chen, Sibo Zhao, Dan Wang, Huijuan Wang, Yanpeng Jia, Ling Shen, Yonggui Yuan, Honghong Yao","doi":"10.1002/adhm.202402219","DOIUrl":"https://doi.org/10.1002/adhm.202402219","url":null,"abstract":"Major depressive disorder (MDD) is a prevalent mental disorder that significantly impacts social and psychological function, but no effective medication is currently available. Circular RNAs (circRNAs) have been reported to participate in the pathogenesis of MDD which are envisioned as promising therapeutic targets. However, nonviral‐based delivery strategies targeting circRNA against MDD are not thoroughly investigated. Here, it is identified that circATF7IP is significantly upregulated in plasma samples and positively correlated with 24‐Hamilton Depression Scale (HAMD‐24) scores of MDD patients. Synergistic amine lipid nanoparticles (SALNPs) are designed to deliver siRNA targeting circATF7IP (si‐circATF7IP) into the hippocampus brain region by intranasal administration. Intranasal delivery of SALNP‐si‐circATF7IP successfully alleviated the depressive‐like behaviors in the LPS‐induced mouse depression model via decreasing CD11b<jats:sup>+</jats:sup>CD45<jats:sup>dim</jats:sup> microglia population and pro‐inflammatory cytokine productions (TNF‐α and IL‐6). These results indicate that the level of circATF7IP positively correlates with MDD pathogenesis, and SALNP delivery of si‐circATF7IP via intranasal administration is an effective strategy to ameliorate LPS‐induced depressive‐like behaviors.","PeriodicalId":113,"journal":{"name":"Advanced Healthcare Materials","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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