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The Role of Nu-Film-17 Spreader in Improvement Droplets Distribution and Increasing Insecticidal Activity of Certain Insecticides on The Spiny Bollworm, Earias insulana ON Cotton Plants in Egypt. nu -17喷雾剂在改善埃及棉铃虫飞沫分布和提高某些杀虫剂杀虫活性中的作用
Pub Date : 2020-06-01 DOI: 10.21608/eajbsf.2020.81532
R. Dar
Field experiments were carried out of about 14 karats planted with (Giza 86) cotton variety during seasons 2018 and 2019 on 15th  July and 1st, 15th August in a field located at Qaha Research Station, Plant Protection  Research Institute, Qalyoubia governorate. The selected area was split into 6 plots and the control plot. Two alternative products include Chlorpyriphos (OP's), and Chlorflurzoran (IGR's) were sprayed by using Rotary Matabi sprayer (18 L./fed.) on cotton field highly infested with cotton Spiny bollworm, Earias insulana larvae. All tested Insecticides revealed a significant negative influence on Earias insulana larvae. The most effective on reduction percentages of bolls infestation was Chlorpyriphos withNU-FILM -17 SPREADER®1% ,5% followed by Chlorflurzoran withNU-FILM -17 SPREADER® 1% ,5%    with Rotary Matabi sprayer (18 L./fed.). It could be recommended to use these Insecticides with Low Volume spraying equipment with not less than (18L/Fed.). A satisfactory coverage was obtained on cotton plants. The spectrum of droplets ranging between 120-170 mm (VMD). With sufficient number ranging from 13-265 N/cm². The rate of performance of Rotary Matabi sprayer (18 L./fed.). was 2.4 Fed./day. The results indicated that there was no significant difference between using NU-FILM -17 SPREADER®   1% or 5% in reduction percentages of bolls infestation. Using both Chlorpyriphos and Chlorflurzoran with NU-FILM -17 SPREADER® 1%   could be recommended in controlling Spiny bollworm, Earias insulana larvae because it increased droplets numbers 54%, decreased droplet sizes 17 % and minimized lost spray on ground 44% which made good spray quality, homogeneity and spray coverage.  Also, Low Volume spraying reducing the time lost in the process filling the machines with the spray solution and saving the lost spray on the ground certainly in small plantation around villages.
于7月15日和8月1日、15日在Qalyoubia省植物保护研究所Qaha研究站的田间进行了2018和2019两季(吉萨86)棉花品种约14克拉的田间试验。选取的区域分为6个样区和对照样区。采用18 l /次的旋转式马塔比喷雾器,在棉铃虫幼虫高发的棉田上喷洒灭虫灵(OP’s)和氟虫灵(IGR’s)。所有杀虫剂对岛小蠹幼虫均有显著的负面影响。对棉铃侵染效果最好的是杀虫蜱与nu - film -17喷雾器分别为1%、5%、1%、5%,其次是吡虫啉与nu - film -17喷雾器分别为1%、5% (18 l /次)。建议使用不少于(18L/Fed)的小容量喷药设备。在棉花植株上获得了满意的覆盖率。液滴光谱范围在120 ~ 170 mm (VMD)之间。足够的数量范围为13-265 N/cm²。旋转玛塔比喷雾机(18升/次)的性能。为2.4次/天。结果表明,施用1%和5%的NU-FILM -17播种剂对棉铃侵染的减少率无显著差异。毒死蜱和氯氟唑啉与1%的nuf - film -17 SPREADER®混合使用,可使飞沫滴数增加54%,飞沫大小减少17%,地面损失减少44%,具有良好的喷雾质量、均匀性和覆盖效果。此外,小批量喷洒减少了在喷洒过程中浪费的时间,并节省了在村庄周围的小种植园中浪费的喷雾。
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引用次数: 0
Analysis of Fenpyroximate Residues in Eggplant, Aubergine (Solanum melongena L.) During Crop Production Cycle by HPLC and Determination of Its Biological Activity 茄子(Solanum melongena L.)中苯螨酸酯残留量分析作物生产周期中高效液相色谱法及其生物活性测定
Pub Date : 2020-06-01 DOI: 10.21608/eajbsf.2020.87591
Shiry S. Takla, F. El-Dars, A. Amien, M. Rizk
A method for determination of Fenpyroximate (Ortus) (5%) residues in eggplant (Solanum melongena L.) by HPLC is described. The analysis was done using HPLC equipped with a UV-Vis detector at 254 nm.  The degradation rate of Fenpyroximate was studied and the results indicated that final residue in eggplant reached 20.1% after 21 days which was considered safe for human and animal consumption, despite its insecticide effectively on eggplant pests.
建立了用高效液相色谱法测定茄子(Solanum melongena L.)中芬吡肟(Ortus)(5%)残留量的方法。采用高效液相色谱(HPLC)和254 nm紫外可见检测器进行分析。对苯吡肟的降解率进行了研究,结果表明,苯吡肟在茄子中的最终残留在21天后达到20.1%,可以安全食用,尽管其对茄子害虫有有效的杀灭作用。
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引用次数: 2
Insecticide resistance and efficacy evolved in field populations of Spodoptera littoralis in Egypt cotton season 埃及棉季沿海夜蛾田间种群对杀虫剂的抗性和药效变化
Pub Date : 2020-06-01 DOI: 10.21608/eajbsf.2020.75972
El-Shabrawy Mona, S. Hafez, A. Reham
This study aimed mainly to estimate the resistance of the cotton leafworm Spodoptera littoralis to several bioinsecticides Agerine 6.5% WP, Protecto 9.4 %WP biotect 9.4 %, wp Dipel 2x 6.4% WP, Dipel Df 5.4 % WG and two other bioinsecticides,  Emamectin benzoate (Radical 0.5 %) EC against Spodoptera littoralis that all field strains the tested bioinsecticides used against cotton leafworm S. littoralis were calculated for both susebtable and field strains collected from six governorates namely Sharkia, Dakahlia, Behera, kafr- Elshek, Fayuom and Beni- swef in the cotton season 2019. The resistance ratio fluctuated from very low resistance to low resistance in some governorates to others except low of governorates RR indicated high resistance. tested IGR, s used against cotton leafworm S. littoralis were calculated for both susebtable and field strains collected from six governorates namely Sharkia, Dakahlia, Behera, kafr- Elshek, Fayuom, and Beni-Swef in the cotton season 2019. showed no resistance in all tested IGR, s for all Governorates, except some Governorate in some IGR, s
本研究主要评价了棉花叶虫对Agerine 6.5% WP、Protecto 9.4% WP、bitect 9.4%、WP Dipel 2x 6.4% WP、Dipel Df 5.4% WG等几种生物杀虫剂的抗性。对2019年棉花季从Sharkia、Dakahlia、Behera、kafr- Elshek、Fayuom和Beni- swef 6个省采集的所有用于防治沿海夜蛾的生物杀虫剂的易感和田间菌株进行了计算。在一些省份,抗性比从极低到低波动,而在其他省份,抗性比低表明抗性高。对2019年棉花季从Sharkia、Dakahlia、Behera、kafr- Elshek、Fayuom和Beni-Swef 6个省采集的棉花叶虫敏感品系和田间品系的IGR检测值进行了计算。除某些IGR中的某些省份外,所有省份检测的所有IGR均未显示耐药性
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引用次数: 3
Bioefficacy of Farnesol, A Common Sesquiterpene, On the Survival, Growth, Development, and Morphogenesis of Spodoptera littoralis (Lepidoptera: Noctuidae). 一种常见倍半萜法尼醇对沿海夜蛾生存、生长、发育和形态发生的生物功效。
Pub Date : 2020-06-01 DOI: 10.21608/eajbsf.2020.78671
G. K., Hamadah, Kh., W. H.
Egyptian cotton leafworm Spodoptera littoralis (Boisduval) is a dangerous pest of many field crops and vegetables in the world. The present study was conducted to evaluate the toxicity of Farnesol and its effects on the growth, development, and morphogenesis of this insect. The newly moulted larvae of 5th (penultimate) or 6th (last) instar larvae were fed on castor bean leaves previously treated with 7 concentrations of Farnesol (400, 200, 100, 50, 25, 12.5 & 6.25 ppm) for 24 hr. The most important results could be summarized as follows. After treatment of 5th or 6th instar larvae with Farnesol, various mortalities were recorded among larvae, pupae, and adults. Depending onLC50values, Farnesol exhibited stronger insecticidal activity after treatment of 6th instar larvae(LC50= 33.67 ppm) than after treatment of 5th instar larvae(LC50= 36.56 ppm). Farnesol caused a serious reduction of larval weight gain and deleterious regression of the growth rate. The larval and pupal durations had been remarkably prolonged, in a dose-dependent course. Disruption of the developmental program was recorded as a failure of ecdysis after treatment of 5th instar larvae and production of larval-pupal intermediates, regardless the treated larval instar. Farnesol exerted considerable suppressing action on the pupation. At higher concentrations, Farnesol interfered with the adult emergence, since eclosion was completely prevented at the highest concentration and partially blocked at other concentrations. Irrespective of the treated larval instar, some deformed pupae were developed only at higher two concentrations of Farnesol.
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引用次数: 6
Effects of Chemical and Biological Anti-mycotoxins on Performance, Haematological, Biochemical and Immunological Parameters of Broiler Chickens during Aflatoxicosis 化学和生物抗真菌毒素对黄曲霉中毒期间肉鸡生产性能、血液学、生化和免疫指标的影响
Pub Date : 2020-06-01 DOI: 10.21608/eajbsf.2020.84488
Omnia E. Kilany, Rania Abdou Helmi, I. Fares, Manal A M Mahmoud
Background:Contamination of feedstuffs with mycotoxins is a worldwide problem of great importance. Mycotoxins are secondary toxic fungal metabolites. There are great interests to create effective prevention and decontamination methods to diminish the toxic effects of AFs in animal and poultry production. Objectives: The present study was designed to evaluate the toxic effects of aflatoxin on performance, some hematological, serum biochemical and immunological parameters and to determine the preventive effect of added anti-mycotoxins. Methods:In this study, a total of 180 broilers were used and divided into 6 equal groups. They received zeocem (chemical synthetic anti-mycotoxin) at a dose of 1 kg/ton feed and nutritox (biological synthetic anti-mycotoxin) at a dose of 0.25 kg/ton feed, to treat broiler fed aflatoxin. All treatments were administered from 1- 42 days of age. Results: By the end of the experiment, nutritox (biological synthetic anti-mycotoxin) alleviated the hazardous effects of aflatoxin on performance, hematological, serum biochemical and immunological parameters rather than zeocem (chemical anti-mycotoxin) fed broilers. Conclusion:Treatment with biological synthetic anti-mycotoxin (nutritox) is better than using chemical synthetic anti-mycotoxin (zeocem) in control of aflatoxicosis in broilers.
背景:真菌毒素污染饲料是一个世界性的重要问题。真菌毒素是次生毒性真菌代谢物。创造有效的预防和净化方法以减少AFs在畜禽生产中的毒性作用是非常有意义的。目的:评价黄曲霉毒素对仔猪生产性能、血液学、血清生化及免疫学指标的影响,并确定添加抗霉菌毒素的预防作用。方法:选用180只肉鸡,随机分为6组。他们分别给饲喂黄曲霉毒素的肉鸡注射1公斤/吨饲料剂量的zeocem(化学合成抗真菌毒素)和0.25公斤/吨饲料剂量的nutritox(生物合成抗真菌毒素)。所有治疗均在1 ~ 42日龄进行。结果:在试验结束时,生物合成抗真菌毒素nutriitox缓解了黄曲霉毒素对肉鸡生产性能、血清学、血清生化和免疫指标的影响,而化学抗真菌毒素zeocem则缓解了黄曲霉毒素对肉鸡生产性能、血清学、血清生化和免疫指标的影响。结论:生物合成抗真菌毒素(营养毒素)治疗肉鸡黄曲霉中毒效果优于化学合成抗真菌毒素(zeocem)。
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引用次数: 3
Biochemical Effects of Some Chitin Synthesis Inhibitors Against Red Palm Weevil, Rhynchophorus ferrugineus Insect 几种几丁质合成抑制剂对红棕象鼻虫的生化作用
Pub Date : 2020-06-01 DOI: 10.21608/eajbsf.2020.83542
A. El-Sobki, A. Ali
The widespread use of the insecticides for controlling the destructive pest, red palm weevil (Rhynchophorus ferrugineus), raises the question about efficacy the chitin synthesis inhibitors (CSIs). Therefore, this study aims to explore the tested CSIs acute toxicity on the field strain of red palm weevil. Also, some biochemical changed in the 6th instar larvae after treatment with tested LC50s were measured. The LC50 values of chlorfluazuron, hexaflumuron, and lufenuron were determined at 454.01,1293.02 and 919.13 µg/ml, respectively for the 6th larval instar exposed using the food dipping method. The lufenuron showed the lowest significant reduction in total protein (7.29%) accompanied with activation GST, s-esterase, carboxylesterase, phenoloxidase, chitinase, and ɑ-Esterase recorded 58.50, 58.54, 26.09, 22.56, 11.52 and 7.77% increasing respectively. The chlorfluazuron showed mediated significant reduction in total protein (23.54%) accompanied with activation GST, s-esterase and carboxylesterase and chitinase recorded 24.90, 23.94, 4.89 and 2.28 % increasing respectively. The highest significant reduction in total protein (45.96%) with hexaflumuron treatment accompanied with activation GST, carboxylesterase and chitinase recorded 48.02, 38.86 and 25.22% increase respectively.
随着红棕榈象鼻虫(Rhynchophorus ferrugineus)杀虫剂的广泛使用,人们对几丁质合成抑制剂(CSIs)的有效性提出了质疑。因此,本研究旨在探讨经测试的CSIs对红棕榈象鼻虫田间品系的急性毒性。测定了lc50处理后6龄幼虫的生化变化。用浸食法测定6龄幼虫对氯氟脲、六氟脲和氟虫腈的LC50分别为454.01、1293.02和919.13µg/ml。氟虫腈对总蛋白的降低幅度最小(7.29%),对GST、s-酯酶、羧酸酯酶、酚氧化酶、几丁质酶和β -酯酶的激活分别达到58.50%、58.54%、26.09、22.56%、11.52%和7.77%。氯氟脲介导总蛋白显著降低(23.54%),GST、s-酯酶、羧酸酯酶和几丁质酶分别升高24.90%、23.94%、4.89%和2.28%。六氟脲隆同时激活GST、羧酸酯酶和几丁质酶,总蛋白降低率最高(45.96%),分别为48.02、38.86和25.22%。
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引用次数: 4
The Joint Action of Entomopathogenic Nematodes Mixtures and Chemical Pesticides on Controlling Helicoverpa armigera (Hübner) 混合虫媒线虫与化学农药防治棉铃虫的联合作用
Pub Date : 2020-06-01 DOI: 10.21608/eajbsf.2020.80376
R. El-Ashry, Mohamed Ali, Abdelhadi A I Ali
Chemical pesticides are characterized by the rapid impact effect in reducing pest population, while, microbial pesticides need along the latent period to cause a limited reduction of the pest population. Low efficiency of biocides may be due to low compatibility with agrochemicals or poor application of biocide, so, the study carried out to evaluate the possibility of mixing five common chemical insecticides and nematicides with five compatible entomopathogenic nematodes (EPNs). For the control of tomato fruit borer (Helicoverpaarmigera),bioassay revealed that pesticide chlorpyrifos (Pestban 48% EC), chlorpyrifos (Tafaban 48% EC) and flubendiamide (Takumi 20% WG) were applied at the recommended dose.  Flubendiamide was less toxic to EPNs infective juveniles compared to fenamiphos (Dento 40% EC). Moreover, steinernematid species were more sensitive than heterorhabditid species to pesticides recording 49.45% and 43.76%, respectively, after 7 days of exposure. The joint action of tested chemical pesticides with IJs of EPNs in controlling the 5th instar larvae of tomato fruit wormshowed an additive or antagonistic reaction with no evidence of synergistic action. Antagonism reaction was recorded with all fenamiphos combinations;the combination ofabamectin and S. feltiae as well as H. bacteriophora (Ba-1), in addition to, flubendiamide combinations with S. feltiae and H. bacteriophora (Ba-1). While, an additive effect was observed in flubendiamide combinations with S. glaseri, S. carpocapsae and H. bacteriophora (HP 88). In the greenhouse experiment, the application of EPNs alone caused mortality ranged from 28 to 36% for 5th instar larvae. Whereas, the highest larval mortality was observed in descending order for combinations between H. bacteriophora (HP88 strain) with fenamiphos (64.0%), chlorpyrifos (54%) and abamectin (54.0%), while, local isolate, H. bacteriophora (Ba-1 strain) achieved mortality ranged from 40 to 50 % with the tested pesticides. Overall, results indicate the feasibility of the integrated use of these nematode species and chemical pesticides in crop protection.
化学农药的特点是减少有害生物种群的影响作用迅速,而微生物农药需要沿潜伏期才能引起有限的减少有害生物种群。杀菌剂的效率低可能是由于与农用化学品相容性低或杀菌剂施用不当造成的,因此,本研究对5种常见化学杀虫剂和杀线虫剂与5种相容的虫病线虫(epn)混合使用的可能性进行了评价。为防治番茄果螟(helicoverpanmigera),生物测定结果显示,按推荐剂量施用毒死蜱(Pestban 48% EC)、毒死蜱(Tafaban 48% EC)和氟虫胺(Takumi 20% WG)。氟苯双胺对感染epn的幼鱼的毒性较氟苯双胺低(Dento 40% EC)。暴露7 d后,拟虫对农药的敏感性分别为49.45%和43.76%,高于异虫。化学农药与EPNs的IJs对番茄果虫5龄幼虫的联合防治表现为加性或拮抗作用,无增效作用。除氟苯双胺与feltiae和H. bacteriophora (Ba-1)联用外,还记录了所有非那米磷联用、阿维菌素与feltiae和H. bacteriophora (Ba-1)联用的拮抗反应。氟苯双胺与glaseri s.s、carpocapsae s.s和bacteriophora (hp88)联用有加性效应。在温室试验中,单独施用EPNs对5龄幼虫的死亡率为28% ~ 36%。其中,与氟虫磷(64.0%)、毒死蜱(54%)和阿维菌素(54.0%)配用的噬菌体(HP88)幼虫死亡率最高,与当地分离菌株Ba-1的幼虫死亡率在40% ~ 50%之间。综上所述,这些线虫与化学农药在作物保护中综合利用是可行的。
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引用次数: 1
Toxicological Aspects of Physiological and Biochemical Changes with Potassium Silicate and Silica Nano-Particles on Albino Rat 硅酸钾和二氧化硅纳米颗粒对白化大鼠生理生化变化的毒理学研究
Pub Date : 2019-12-31 DOI: 10.21608/eajbsf.2019.136679
H. El-Bendary
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引用次数: 0
Toxicity Effects and Biochemical Changes of Insecticide Alternatives on Cowpea Aphid, (Aphis craccivora) (Homoptera: Aphididae) 不同杀虫剂对豇豆蚜虫(同翅目:蚜虫科)的毒力及生化变化
Pub Date : 2019-10-01 DOI: 10.21608/eajbsf.2019.67373
A. Shehawy, A. Khalil, A. Maklad, Sameer H Qari
The cowpea aphid; Aphis craccivora is one of the harmful pests, which infest cowpea bean, Faba bean plants and many crops leading to a decrease of crop production. Plants including; Citrullus colocynthis, Peganum harmala, Senna alexandrina, andApium graveolens wereextracted bymethanol 70%, in order to evaluate its toxicity effect on Aphis craccivora in laboratory and field, as well as Alkaline phosphatase, non-specific esterase and mixed-function oxidase were evaluated after treatment with LC50 of different plant extracts. The results showed that LC50 was 4890.079,5537.761, 6118.4856 and 6118.4856 ppm respectively, while it was 74.1996 ppm for Pirimicarb. Moreover, the combination of the different plant extracts showed or exhibit such a dramatic reduction in A. craccivora in the field and minimize the concentration of the pirimicarb required to reduce the aphid population in the field. Also, P. harmala, S. alexandrina showed an increase in Alkaline phosphatase, non-specific esterase and mixed-function oxidase, while it was moderate for C. colocynthis and low for A. graveolens compared with the control. These results should be helpful in the rational applications of insect pest management.
豇豆蚜虫;蚜虫是一种有害害虫,主要危害豇豆、蚕豆和多种作物,造成作物减产。植物包括;采用70%甲醇提取甜瓜(Citrullus colocynthis)、苦瓜(Peganum harmala)、山黧豆(Senna alexandrina)和石首花(apium graveolens),在实验室和田间评价其对小蚜虫的毒性作用,并以不同植物提取物LC50处理后,评价其对小蚜虫的碱性磷酸酶、非特异性酯酶和混合功能氧化酶的活性。结果表明,吡虫威的LC50分别为4890.079、5537.761、6118.4856和6118.4856 ppm,而吡虫威的LC50为74.1996 ppm。此外,不同植物提取物的组合在田间显示或表现出这样的戏剧性减少,并将减少田间蚜虫种群所需的吡虫威浓度降至最低。与对照相比,绿草假丝草的碱性磷酸酶、非特异性酯酶和混合功能氧化酶的含量均有所增加,而绿草假丝草的含量中等,绿草假丝草的含量较低。这些结果将有助于害虫治理的合理应用。
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引用次数: 4
Evaluation of some Organophosphorous Insecticides against the Tomato Whitefly, Bemisia tabaci (GENN.) Collected from some Upper Egypt Governorates with Relation to Esterases Enzyme Activities 几种有机磷杀虫剂对番茄粉虱、烟粉虱的防治效果评价采集自上埃及一些省份,与酯酶活性有关
Pub Date : 2019-10-01 DOI: 10.21608/eajbsf.2019.57743
S. M. A. El-Naby, T. Elsheikh, E. S. A. E. Mageed
The tomato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) is a worldwide pest of many crops. Organophosphorous (OP) insecticides have been used to control this insect for many years and are still being used despite the development of resistance. In this work’ evaluation of the biological activity of three organophosphorous insecticides, profenofos 72% E.C. (Selecron); chlorpyrifos 48% E.C.(Dursban) and fenitrothion 50% E.C. (Sumithion)  on the felid strain of tomato whitefly was carried out on tomato plants.  Whitefly adults were collected from four different Egyptian Governorates throughout three successive years (2016-2018). The LC50 value of each compound in each Governorate was used to determine the biochemical responses of this insect pest.The colorimetric test of esterase activity is a useful tool to detect the level of resistance against this group of insecticides. The obtained results showed that profenofos insecticide was more toxic on the tested insects followed by chlorpyrifos, while fenitrothion showed a low toxic effect on the tested insects in all years. The activities of α and β esterases have fluctuated up and down in some Governorates.
番茄粉虱(半翅目:粉虱科)是危害多种作物的世界性害虫。有机磷(OP)杀虫剂多年来一直用于控制这种昆虫,尽管出现了抗药性,但仍在使用。本文对三种有机磷杀虫剂的生物活性进行了评价:72%丙烯磷;在番茄植株上对田间番茄粉虱品系施用48%毒死蜱(德士邦)和50%杀虫磷(苏密松)。在连续三年(2016-2018年)期间,从埃及四个不同的省份收集了成年白蛉。采用各省各化合物LC50值测定该害虫的生化反应。酯酶活性比色试验是检测对该类杀虫剂抗性水平的有效手段。结果表明,不同年份对被试昆虫的毒副作用均以毒死蜱次之,毒死蜱次之,杀虫硫磷对被试昆虫的毒副作用较低。α和β酯酶的活性在一些省份上下波动。
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引用次数: 0
期刊
Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control
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