Pub Date : 2022-04-01DOI: 10.21608/ejah.2022.240044
A. Abd-elaziz, Safaa H. Aboolo
O riganum vulgare essential oil (OEO) was added in diets of Basa fish ( Pangasius hypophthalmus ) at levels of 0.0, 5.0, 10.0, 15.0, and 20.0 g /kg feed to determine its effects on antioxidative capacity, immunity, histopathology, expression of immune-related genes and resistance to Aeromonas hydrophila . After 2 months of feeding experiment, serum lysozyme, superoxide dismutase and catalase enzymes have been dramatically increased according to the levels of added (OEO) , the highest level was at group fed on 20 gm /kg feed and 15gm/kg feed while, the lowest level at the control group also the expression of interleukin-1beta and toll-like receptor 2 genes in kidney tissues have been increased as the same manner .OEO-supplemented diet pereserves the normal architecture of liver, gills, intes-tines, and kidneys in all experimental groups. The scores of the histopathological lesions were significantly decreased with presence of regeneration to damaged cells in groups fed 20 gm OEO/kg feed &15 gm OEO/kg feed. After pathogenic A. hydrophila challenge the mortality percent after 10 days of the challenge experiment promptly reduced and the percent of survival of Basa fish was relatively increased by OEO administration according to the given dose . Findings show that the OEO in diet has improved the antioxidative status, immunological responses, histopathology, evaluation of immune related genes expression of Basa
本试验在低眼巴沙鱼饲料中分别添加0.0、5.0、10.0、15.0和20.0 g /kg饲料，研究其对巴沙鱼抗氧化能力、免疫、组织病理学、免疫相关基因表达和对嗜水气单胞菌抗性的影响。经过2个月的饲养试验、血清溶菌酶、超氧化物歧化酶和过氧化氢酶酶已经极大地提高了根据添加的水平(OEO)的最高水平是在美联储在20国集团通用/公斤饲料和15通用/公斤饲料,最低水平在对照组也interleukin-1beta和toll样受体2基因的表达在肾组织增加相同的方式.OEO-supplemented饮食pereserves肝脏的正常结构,腮,所有实验组的肠道和肾脏。饲喂20 gm OEO/kg饲料和15 gm OEO/kg饲料组的组织病理学病变评分显著降低，存在损伤细胞再生。病原性嗜水单胞菌攻毒后，在攻毒实验10 d后，按一定剂量给药OEO可迅速降低巴沙鱼的死亡率，相对提高巴沙鱼的存活率。结果表明，饲粮中添加OEO改善了Basa的抗氧化状态、免疫反应、组织病理学和免疫相关基因表达评价
{"title":"Effect of dietry Origanum vulgare extract on Immunological responses of Basa fish (Pangasius hypophthalmus) against Aeromonas hydrophila infection.","authors":"A. Abd-elaziz, Safaa H. Aboolo","doi":"10.21608/ejah.2022.240044","DOIUrl":"https://doi.org/10.21608/ejah.2022.240044","url":null,"abstract":"O riganum vulgare essential oil (OEO) was added in diets of Basa fish ( Pangasius hypophthalmus ) at levels of 0.0, 5.0, 10.0, 15.0, and 20.0 g /kg feed to determine its effects on antioxidative capacity, immunity, histopathology, expression of immune-related genes and resistance to Aeromonas hydrophila . After 2 months of feeding experiment, serum lysozyme, superoxide dismutase and catalase enzymes have been dramatically increased according to the levels of added (OEO) , the highest level was at group fed on 20 gm /kg feed and 15gm/kg feed while, the lowest level at the control group also the expression of interleukin-1beta and toll-like receptor 2 genes in kidney tissues have been increased as the same manner .OEO-supplemented diet pereserves the normal architecture of liver, gills, intes-tines, and kidneys in all experimental groups. The scores of the histopathological lesions were significantly decreased with presence of regeneration to damaged cells in groups fed 20 gm OEO/kg feed &15 gm OEO/kg feed. After pathogenic A. hydrophila challenge the mortality percent after 10 days of the challenge experiment promptly reduced and the percent of survival of Basa fish was relatively increased by OEO administration according to the given dose . Findings show that the OEO in diet has improved the antioxidative status, immunological responses, histopathology, evaluation of immune related genes expression of Basa","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86778277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.21608/ejah.2022.252288
Eman I. M. Beleta, Rania I. Ismail, Hend I. Elsharkawy
T his study reported on a sporadic, naturally acquired infection of sheep and goats with Brucella on a private farm in El- Menofya Gover-norate, Egypt. The abortions, which occurred in a flock of 46 sheep and 33 goats, involved six ewes and five does at the third month of gesta-tion. Serum samples from the flock were examined for Brucella antibodies using rose bengal test (RBT), Tube Agglutination Test (TAT) , indirect Enzyme Linked Immunosorbent Assay (I ELISA) and were further confirmed using complement fixation test (CFT) gave 18(39.1%), 16(34.8%), 19 (41.3%), and 17(37%) in sheep; respectively, while in goats revealed 11 (33.3%), 8(24.2%), 12(36.4%), and 11(33.3%) respectively. Tissue samples were collected from 28 positive animals, as detected by CFT, at slaughter-ing. The bacteriological results revealed 44 isolates which were biochemi-cally identified as B. melitensis biovar3. The highest recovery rate was obtained from supra mammary lymph nodes (22/28; 78.6%), followed by spleen (15/28; 53.6%), and finally liver (7/28; 25%).The distribution of the virulence genes among 44 B. melitensis isolates revealed that Omp 25 recorded the highest incidence 44(100%), then followed wbkA 43(97.7%) and manB 42 (95.5%) . The high prevalence of virulence-associated genes among the B. melitensis
{"title":"Bacteriological and molecular characterization of Brucella isolates from sheep and goats","authors":"Eman I. M. Beleta, Rania I. Ismail, Hend I. Elsharkawy","doi":"10.21608/ejah.2022.252288","DOIUrl":"https://doi.org/10.21608/ejah.2022.252288","url":null,"abstract":"T his study reported on a sporadic, naturally acquired infection of sheep and goats with Brucella on a private farm in El- Menofya Gover-norate, Egypt. The abortions, which occurred in a flock of 46 sheep and 33 goats, involved six ewes and five does at the third month of gesta-tion. Serum samples from the flock were examined for Brucella antibodies using rose bengal test (RBT), Tube Agglutination Test (TAT) , indirect Enzyme Linked Immunosorbent Assay (I ELISA) and were further confirmed using complement fixation test (CFT) gave 18(39.1%), 16(34.8%), 19 (41.3%), and 17(37%) in sheep; respectively, while in goats revealed 11 (33.3%), 8(24.2%), 12(36.4%), and 11(33.3%) respectively. Tissue samples were collected from 28 positive animals, as detected by CFT, at slaughter-ing. The bacteriological results revealed 44 isolates which were biochemi-cally identified as B. melitensis biovar3. The highest recovery rate was obtained from supra mammary lymph nodes (22/28; 78.6%), followed by spleen (15/28; 53.6%), and finally liver (7/28; 25%).The distribution of the virulence genes among 44 B. melitensis isolates revealed that Omp 25 recorded the highest incidence 44(100%), then followed wbkA 43(97.7%) and manB 42 (95.5%) . The high prevalence of virulence-associated genes among the B. melitensis","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88961667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.21608/ejah.2022.246348
{"title":"Monitoring the antimicrobial resistance of Salmonella and Escherichia coli in chickens and migratory birds","authors":"","doi":"10.21608/ejah.2022.246348","DOIUrl":"https://doi.org/10.21608/ejah.2022.246348","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"67 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91498220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.21608/ejah.2022.240650
El Asuoty, M. Gerges
C hicken meat is commonly marketed at refrigerated temperatures (25oC). The major concern for retailers and consumers is the quality and safety of refrigerated chicken meat. During chilling period, chicken meat undergoes many undesirable changes due to microbial growth that lead to spoilage and economic loss. Therefore, this study was conducted to evaluate the effect of pomegranate juice (PJ) in three concentrations (1, 2, and 4%) on sensory attributes, chemical and microbiological quality of chicken breast stored at 4±1oC for 12 days. The results showed that dipping of chicken breast meat samples in PJ at three concentrations 1, 2 and 4% can improve storage stability of chicken breast samples. This study also concluded that the use of PJ at a concentration of 4% is more effective compared to concentrations of 1% and 2%. Therefore, PJ could be used as a natural antioxidant and antimicrobial preservative for chilled chicken meat held at refrigerated temperature.
{"title":"Usage of concentrated pomegranate to extend the shelf life of chicken breast","authors":"El Asuoty, M. Gerges","doi":"10.21608/ejah.2022.240650","DOIUrl":"https://doi.org/10.21608/ejah.2022.240650","url":null,"abstract":"C hicken meat is commonly marketed at refrigerated temperatures (25oC). The major concern for retailers and consumers is the quality and safety of refrigerated chicken meat. During chilling period, chicken meat undergoes many undesirable changes due to microbial growth that lead to spoilage and economic loss. Therefore, this study was conducted to evaluate the effect of pomegranate juice (PJ) in three concentrations (1, 2, and 4%) on sensory attributes, chemical and microbiological quality of chicken breast stored at 4±1oC for 12 days. The results showed that dipping of chicken breast meat samples in PJ at three concentrations 1, 2 and 4% can improve storage stability of chicken breast samples. This study also concluded that the use of PJ at a concentration of 4% is more effective compared to concentrations of 1% and 2%. Therefore, PJ could be used as a natural antioxidant and antimicrobial preservative for chilled chicken meat held at refrigerated temperature.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88572441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.21608/ejah.2022.252183
S. El-Nagar, Azhar G. Shalaby, H. Badr
: C lostridium perfringens is one of the highest dominant spore-forming foodborne pathogens, which is widely distributed and causes severe diseases in humans and animals. Poultry is the main host of this pathogen. In this study, we investigated the prevalence, antibiotic resistance pattern, and toxin-encoding genes of C. perfringens isolated from chicken with necrotic enteritis. Twelve of C. perfringens strains were isolated from 100 intestinal samples (Native and Hubbard chickens) with a percentage of 12% and subsequently confirmed by PCR; all strains carried alpha-toxin gene and were negative for other major toxin genes (Beta, Epsilon and Iota). The C. perfringens isolates showed highly resistance (100%) to most of the antibiotics tested; Ampicillin, Amoxicillin, Amoxicillin-clavulanic acid, Erythromycin, Lincomycin, Metronidazole, Neomycin, Oxytetracycline, Penicillin, Spectinomycin, Streptomycin, but showed sensitivity with a low percentage (25% or low) for Colistin sulphate, Doxycyclin, Enrofloxacin, Norfloxacin and Sulfamethoxazole-trimethoprim. However, virulence genes were detected with a different percentage of 100%, 75% and 16.7 with Cpe , netB and Tpel respectively. In the examination of antimicrobial-resistant genes the examined isolates showed 100%, 75% and 25%
产气荚膜梭菌是最具优势的产芽孢食源性致病菌之一，分布广泛，在人畜中引起严重疾病。家禽是这种病原体的主要宿主。在这项研究中，我们研究了从鸡坏死性肠炎中分离的产气荚膜荚膜杆菌的流行程度、抗生素耐药性模式和毒素编码基因。12 c . 100年肠道perfringens菌株样本(本地和哈伯德鸡)占12%,随后证实了PCR;所有菌株均携带α -毒素基因，其余主要毒素基因(β、Epsilon和Iota)均阴性。c . perfringens隔离显示高度抵抗大多数测试的抗生素(100%);氨苄西林、阿莫西林、阿莫西林-克拉维酸、红霉素、林可霉素、甲硝唑、新霉素、土霉素、青霉素、大观霉素、链霉素，但对硫酸粘菌素、多西环素、恩诺沙星、诺氟沙星和磺胺甲恶唑-甲氧苄啶的敏感性较低(25%或较低)。Cpe、netB和Tpel的毒力基因检出率分别为100%、75%和16.7%。在抗菌素耐药基因检测中，所检分离株的耐药率分别为100%、75%和25%
{"title":"Antibiotic resistance pattern of Clostridium perfringens isolated from necrotic enteritis cases of broiler chickens in Luxor city","authors":"S. El-Nagar, Azhar G. Shalaby, H. Badr","doi":"10.21608/ejah.2022.252183","DOIUrl":"https://doi.org/10.21608/ejah.2022.252183","url":null,"abstract":": C lostridium perfringens is one of the highest dominant spore-forming foodborne pathogens, which is widely distributed and causes severe diseases in humans and animals. Poultry is the main host of this pathogen. In this study, we investigated the prevalence, antibiotic resistance pattern, and toxin-encoding genes of C. perfringens isolated from chicken with necrotic enteritis. Twelve of C. perfringens strains were isolated from 100 intestinal samples (Native and Hubbard chickens) with a percentage of 12% and subsequently confirmed by PCR; all strains carried alpha-toxin gene and were negative for other major toxin genes (Beta, Epsilon and Iota). The C. perfringens isolates showed highly resistance (100%) to most of the antibiotics tested; Ampicillin, Amoxicillin, Amoxicillin-clavulanic acid, Erythromycin, Lincomycin, Metronidazole, Neomycin, Oxytetracycline, Penicillin, Spectinomycin, Streptomycin, but showed sensitivity with a low percentage (25% or low) for Colistin sulphate, Doxycyclin, Enrofloxacin, Norfloxacin and Sulfamethoxazole-trimethoprim. However, virulence genes were detected with a different percentage of 100%, 75% and 16.7 with Cpe , netB and Tpel respectively. In the examination of antimicrobial-resistant genes the examined isolates showed 100%, 75% and 25%","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83028071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-01DOI: 10.21608/ejah.2022.236770
S. Hazem, Rania I. Ismail, Hend I. Elsharkawy, Eman I. M. Beleta, Abeer A. E. Mohamed
T his study was conducted on different dairy buffalo herds. A total of 316 buffaloes including 290 buffaloes suffering from reproductive disorders and 26 apparently healthy buffaloes in different rural areas belonging to the Al-Qalyubia Governorate were employed in this study. Epidemiological data revealed that these animals had no history of vaccination against Brucellosis. Blood sera of these animals were subjected to serological assessments using the Rose Bengal plate test (RBPT), Competitive En-zyme Linked Immunosorbent Assay (cELISA), complement fixation test (CFT) in addition to the use of real-time PCR ( rtPCR). MRT and bacteriological examination were performed on milk samples. The obtained results showed that the overall sero-prevalences of brucellosis were recorded as 17.41% , 17.09 % , 16.77% , and 17.09% by RBT, c-ELISA, CFT and rtPCR respectively. B. melitensis biovar 3 was isolated from milk samples 47 (14.87%) It was concluded that for the diagnosis of brucellosis in dairy buffaloes, MRT and RBT remain recommended for screening, CFT is ad-vised for confirming infection in individual animals, and cELISA can be used instead of CFT in some cases. Using rtPCR on serum samples should be regarded as an additional diagnostic technique for detecting and identify-ing Brucella infection and should be considered in conjunction with other serological tests that can progress and conquer the limits of different immunoassays. According to bacteriological testing, B. melitensis biovar 3 is still the prevalent Brucella field strain
{"title":"Detection of Brucella infection in dairy buffaloes using conventional and molecular methods","authors":"S. Hazem, Rania I. Ismail, Hend I. Elsharkawy, Eman I. M. Beleta, Abeer A. E. Mohamed","doi":"10.21608/ejah.2022.236770","DOIUrl":"https://doi.org/10.21608/ejah.2022.236770","url":null,"abstract":"T his study was conducted on different dairy buffalo herds. A total of 316 buffaloes including 290 buffaloes suffering from reproductive disorders and 26 apparently healthy buffaloes in different rural areas belonging to the Al-Qalyubia Governorate were employed in this study. Epidemiological data revealed that these animals had no history of vaccination against Brucellosis. Blood sera of these animals were subjected to serological assessments using the Rose Bengal plate test (RBPT), Competitive En-zyme Linked Immunosorbent Assay (cELISA), complement fixation test (CFT) in addition to the use of real-time PCR ( rtPCR). MRT and bacteriological examination were performed on milk samples. The obtained results showed that the overall sero-prevalences of brucellosis were recorded as 17.41% , 17.09 % , 16.77% , and 17.09% by RBT, c-ELISA, CFT and rtPCR respectively. B. melitensis biovar 3 was isolated from milk samples 47 (14.87%) It was concluded that for the diagnosis of brucellosis in dairy buffaloes, MRT and RBT remain recommended for screening, CFT is ad-vised for confirming infection in individual animals, and cELISA can be used instead of CFT in some cases. Using rtPCR on serum samples should be regarded as an additional diagnostic technique for detecting and identify-ing Brucella infection and should be considered in conjunction with other serological tests that can progress and conquer the limits of different immunoassays. According to bacteriological testing, B. melitensis biovar 3 is still the prevalent Brucella field strain","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80160067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21608/ejah.2022.220757
Hanan A. Tag El Din, Hamada M. Yousif, Reda Rezk
T race element deficiency in male lambs is a serious problem. It refers to either insufficient nutrients or mineral elements imbalance. This work aimed to investigate the hormonal and some biochemical changes related to some trace element deficiency and the effect of mineral mixture supplementation on these changes. To achieve this aim we induced trace element deficiency in a tested male lambs group (N=10) belonged to our sheep herd and received a mineral mixture as a feed supplement for 10 days. The analysis of tested diet revealed protein 12.32%, calcium 1.21%, phosphorus 0.27%, and zinc 25.96 mg/kg DM. While the control healthy group (N=10) received a traditional diet. The results of serum hormones and biochemical analysis of tested group revealed a significant (P˂0.05) decrease in TSH, T3, T3/T4, testosterone, free testosterone, SHBG, GH, albumin and zinc, with a significant (P˂0.05) increase in T4, total protein and globulin compared to control healthy group. On the other hand, the results obtained after mineral mixture supplementation revealed a significant (P˂0.05) increase of TSH, T3, T3/T4, testosterone, free testosterone, SHBG, GH, albumin and zinc. Also, a significant (P˂0.05) decrease in T4, total protein, and globulin was observed compared with this group before mineral mixture supplementation, with observation of the changes were gradually toward recovery. Moreover, insignificant changes were recorded in calcium, phosphorus and iodine. In conclusion to trace element deficiency have an effect on vital hormones with extension to metabolic process, general health condition and proper growth of male lambs.
{"title":"Some hormonal changes related to some trace elements deficiency in ration of male lambs","authors":"Hanan A. Tag El Din, Hamada M. Yousif, Reda Rezk","doi":"10.21608/ejah.2022.220757","DOIUrl":"https://doi.org/10.21608/ejah.2022.220757","url":null,"abstract":"T race element deficiency in male lambs is a serious problem. It refers to either insufficient nutrients or mineral elements imbalance. This work aimed to investigate the hormonal and some biochemical changes related to some trace element deficiency and the effect of mineral mixture supplementation on these changes. To achieve this aim we induced trace element deficiency in a tested male lambs group (N=10) belonged to our sheep herd and received a mineral mixture as a feed supplement for 10 days. The analysis of tested diet revealed protein 12.32%, calcium 1.21%, phosphorus 0.27%, and zinc 25.96 mg/kg DM. While the control healthy group (N=10) received a traditional diet. The results of serum hormones and biochemical analysis of tested group revealed a significant (P˂0.05) decrease in TSH, T3, T3/T4, testosterone, free testosterone, SHBG, GH, albumin and zinc, with a significant (P˂0.05) increase in T4, total protein and globulin compared to control healthy group. On the other hand, the results obtained after mineral mixture supplementation revealed a significant (P˂0.05) increase of TSH, T3, T3/T4, testosterone, free testosterone, SHBG, GH, albumin and zinc. Also, a significant (P˂0.05) decrease in T4, total protein, and globulin was observed compared with this group before mineral mixture supplementation, with observation of the changes were gradually toward recovery. Moreover, insignificant changes were recorded in calcium, phosphorus and iodine. In conclusion to trace element deficiency have an effect on vital hormones with extension to metabolic process, general health condition and proper growth of male lambs.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83172881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21608/ejah.2022.225148
Ehdaa, O. Hamed, A. Zohree
T he present study aimed to monitor the antioxidant and protective effects of Spirulina platensis (SP) against Nanocopper particles (NCPS) which induced liver injuries in rabbits. The experimental animals were randomly divided into three groups, the first group, (control), the second group, (received NCPS, 37.5 mg/kg), and the third group, received NCPS, 37.5 mg/kg bw plus SP, 1.5 g/kg diet for 2 months. After ending the period of the experiment, rabbits were decapitated and, blood, liver, as well as muscle tissue samples were collected for examination of liver function, antioxidant activity, lipid peroxidation, and copper levels. Exposure of rabbits to these Nanoparticles SC for 8 weeks resulted in significant increase levels of glutathione (GSH) in the third group. While hepatic superoxide dismutase (SOD) and malondialdehyde (MAD) activity showed a significant decrease in their levels of the same group. In addition, alanine aminotransferase (ALT) in serum showed a significant decrease. These data revealed that administration of SP protects against Nano copper-induced hepatotoxicity through amelioration of hepatic damage and oxidative stress in rabbits.
{"title":"Effect of Spirulina-Platensis against Nano-copper Toxicity in Rabbit","authors":"Ehdaa, O. Hamed, A. Zohree","doi":"10.21608/ejah.2022.225148","DOIUrl":"https://doi.org/10.21608/ejah.2022.225148","url":null,"abstract":"T he present study aimed to monitor the antioxidant and protective effects of Spirulina platensis (SP) against Nanocopper particles (NCPS) which induced liver injuries in rabbits. The experimental animals were randomly divided into three groups, the first group, (control), the second group, (received NCPS, 37.5 mg/kg), and the third group, received NCPS, 37.5 mg/kg bw plus SP, 1.5 g/kg diet for 2 months. After ending the period of the experiment, rabbits were decapitated and, blood, liver, as well as muscle tissue samples were collected for examination of liver function, antioxidant activity, lipid peroxidation, and copper levels. Exposure of rabbits to these Nanoparticles SC for 8 weeks resulted in significant increase levels of glutathione (GSH) in the third group. While hepatic superoxide dismutase (SOD) and malondialdehyde (MAD) activity showed a significant decrease in their levels of the same group. In addition, alanine aminotransferase (ALT) in serum showed a significant decrease. These data revealed that administration of SP protects against Nano copper-induced hepatotoxicity through amelioration of hepatic damage and oxidative stress in rabbits.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73493062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21608/ejah.2022.226583
Ahmed R. E. Mahdy, Zinab Mohamed
A fter a single oral treatment, the pharmacokinetic characteristics of tylvalosin alone and in combination with either amprolium or toltrazuril in Mycoplasma gallisepticum-infected broiler chickens were evaluated. 18 chickens were divided into 3 equal groups, group one was given tylvalosin only, group two was given tylvalosin and amprolium and group three was given tylvalosin and toltrazuril. The best model for describing tylvalosin serum levels in our study was found to be a twocompartment open model. The maximum serum concentration of tylvalosin was 1.62±0.03, 1.2±0.02, and 1.41±0.01 μg/ml reached at 2.95±0.014, 2.8±0.01, and 2.83±0.02 h in group one, group two, and group three respectively. A significant (P<0.001)increasesin V/F and Cl/F10.19±0.11(mg)/(μg/ ml), 1.54±0.04(mg)/(μg/ml)/h and 8.56±0.07(mg)/(μg/ml), 1.31±0.03 (mg)/ (μg/ml)/h were found in group two and group three, respectively in comparison with group one 7.24 ± 0.06 (mg)/(μg/ml), 1.09±0.02 (mg)/(μg/ml)/h. The absorption and elimination half-lives of tylvalosin were 1.69 ± 0.016 h, 10.63± 0.330h and 1.56± 0.01h, 9.63± 0.41h and 1.58±0.02 h, 9.64±0.35 h in group one, group two, and group three respectively. The area under the curve was significantly (P < 0.001) decreased in group two and group three which were 13.6±0.06 and 15.98±0.14 μg/ml/h respectively, in comparison with group one which was 18.7±0.093 μg/ml/h. It was found that the serum concentration of tylvalosin in group one after twenty-four hours is higher than MIC for the used Mycoplasma gallisepticum (0.25 μg/ml) unlike the second and third group, so it is recommended to give tylvalosin every twenty-four hours if it is given alone while when it is given with amprolium and toltrazuril it should be given every twelve hours.
{"title":"Effect Of Amprolium and Toltrazuril On disposition kinetics Of Tylvalosin In Infected Broiler Chickens With Mycoplasma Gallisepticum","authors":"Ahmed R. E. Mahdy, Zinab Mohamed","doi":"10.21608/ejah.2022.226583","DOIUrl":"https://doi.org/10.21608/ejah.2022.226583","url":null,"abstract":"A fter a single oral treatment, the pharmacokinetic characteristics of tylvalosin alone and in combination with either amprolium or toltrazuril in Mycoplasma gallisepticum-infected broiler chickens were evaluated. 18 chickens were divided into 3 equal groups, group one was given tylvalosin only, group two was given tylvalosin and amprolium and group three was given tylvalosin and toltrazuril. The best model for describing tylvalosin serum levels in our study was found to be a twocompartment open model. The maximum serum concentration of tylvalosin was 1.62±0.03, 1.2±0.02, and 1.41±0.01 μg/ml reached at 2.95±0.014, 2.8±0.01, and 2.83±0.02 h in group one, group two, and group three respectively. A significant (P<0.001)increasesin V/F and Cl/F10.19±0.11(mg)/(μg/ ml), 1.54±0.04(mg)/(μg/ml)/h and 8.56±0.07(mg)/(μg/ml), 1.31±0.03 (mg)/ (μg/ml)/h were found in group two and group three, respectively in comparison with group one 7.24 ± 0.06 (mg)/(μg/ml), 1.09±0.02 (mg)/(μg/ml)/h. The absorption and elimination half-lives of tylvalosin were 1.69 ± 0.016 h, 10.63± 0.330h and 1.56± 0.01h, 9.63± 0.41h and 1.58±0.02 h, 9.64±0.35 h in group one, group two, and group three respectively. The area under the curve was significantly (P < 0.001) decreased in group two and group three which were 13.6±0.06 and 15.98±0.14 μg/ml/h respectively, in comparison with group one which was 18.7±0.093 μg/ml/h. It was found that the serum concentration of tylvalosin in group one after twenty-four hours is higher than MIC for the used Mycoplasma gallisepticum (0.25 μg/ml) unlike the second and third group, so it is recommended to give tylvalosin every twenty-four hours if it is given alone while when it is given with amprolium and toltrazuril it should be given every twelve hours.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87104559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.21608/ejah.2022.230532
El-Gedawy, Yousry A. El-Shazly
P aratuberculosis or Johne’s Disease (JD) is a chronic and incurable granulomatous enteric disease affecting cattle, buffaloes, sheep, goats and the other ruminants caused by M. avium subspecies paratuberculosis (MAP). In this study, A total of 300 cows (240 clinically diseased animals and 60 apparently healthy animals) were examined for the presence of clinical signs of johne's disease including incurable chronic diarrhoea, interment firstly then intense and continuous which is not responding to treatment, emaciation and progressive weakness. Fecal and serum samples (each of 300) were collected from the examined cows housed in 3 Egyptian Governorates (Sharkia, Kalyoubia and Giza). Fecal samples were collected then examined according to the pooling procedure and decontaminated by Hexa decylpyridinium chloride solution (HPC 0.9% prior to culturing on Herrold's Egg Yolk Medium (HEYM). MAP was isolated from 34 of the 60 pooled fecal samples tested (57%). Fecal smears were examined using Ziehl- Neelsen stain (ZN) for the presence of acid fast bacilli revealing 29 fecal smears (48%) of 60 fecal smears were positive. ELISA was conducted on serum samples to detect antibodies against MAP, 212 (71%) of serum samples were positive for antibodies against MAP. Molecular confirmation by PCR IS900 assay was carried out using specific primers directly on fecal sample, Out of the 60 pooled fecal samples, 45 pools (75%) were positive. A phylogenetic analysis for determining the genetic difference between current infection and other infected strains in other localities
{"title":"Bacteriological and Molecular Studies on Johne’s Disease in Cattle","authors":"El-Gedawy, Yousry A. El-Shazly","doi":"10.21608/ejah.2022.230532","DOIUrl":"https://doi.org/10.21608/ejah.2022.230532","url":null,"abstract":"P aratuberculosis or Johne’s Disease (JD) is a chronic and incurable granulomatous enteric disease affecting cattle, buffaloes, sheep, goats and the other ruminants caused by M. avium subspecies paratuberculosis (MAP). In this study, A total of 300 cows (240 clinically diseased animals and 60 apparently healthy animals) were examined for the presence of clinical signs of johne's disease including incurable chronic diarrhoea, interment firstly then intense and continuous which is not responding to treatment, emaciation and progressive weakness. Fecal and serum samples (each of 300) were collected from the examined cows housed in 3 Egyptian Governorates (Sharkia, Kalyoubia and Giza). Fecal samples were collected then examined according to the pooling procedure and decontaminated by Hexa decylpyridinium chloride solution (HPC 0.9% prior to culturing on Herrold's Egg Yolk Medium (HEYM). MAP was isolated from 34 of the 60 pooled fecal samples tested (57%). Fecal smears were examined using Ziehl- Neelsen stain (ZN) for the presence of acid fast bacilli revealing 29 fecal smears (48%) of 60 fecal smears were positive. ELISA was conducted on serum samples to detect antibodies against MAP, 212 (71%) of serum samples were positive for antibodies against MAP. Molecular confirmation by PCR IS900 assay was carried out using specific primers directly on fecal sample, Out of the 60 pooled fecal samples, 45 pools (75%) were positive. A phylogenetic analysis for determining the genetic difference between current infection and other infected strains in other localities","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76449005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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