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Molecular, pathological and hematobiochemical detection of canine parvovirus in dogs 犬细小病毒的分子、病理及血液生化检测
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.305220
Maha M. El Alem, Suzan Salah, Gehan N. Alagmy, Sara A. Gad
: C anine parvovirus (CPV) infection is considered a common cause of puppies mortality less than 6 months. Despite treatment with available therapies, many dogs still died from CPV related complications. This study aims to describe the clinical investigation, pathological findings, and molecular diagnosis of canine parvovirus infection in dogs. A total of thirty five samples, including blood and fecal samples were collected from dogs of different ages and breeds (German shepherd, Husky, Loloo, and Rottweiler puppies) to be used in this study. The study considered the dogs' history, clinical examination for vital parameters, alter-ation of haemogram, and fecal examination for detection of canine parvovirus antigen. 10 rectal swabs collected from dogs showing clinical signs of canine parvovirus enteritis were initially tested for CPV-2 using CPV Ag test kits rapid test. Results showed 9/10 of swabs were positive for CPV-2. For further in-vestigations, all swabs were carried out using molecular identification, through the extraction of Viral DNA and conventional PCR for the CPV-2, all samples gave positive results for CPV-2. Histopathological examination of the tongue, lung, kidney and intestine suggested the infection with CPV enteritis. leukopenia, neutropenia, lymphopenia, and thrombocytopenia with a significant increase in monocytic and eosinophilic count were recorded. A significant increase in serum total protein and serum globulin and a significant decrease in serum albumin were noticed. Serum ALT, AST, blood urea nitrogen (BUN), and serum creatinine were significantly increased. Samples that showed strong PCR products were sequenced and phylogenetic analyzed to detect the percentage of nucleotide sequence identity and divergence between CPV of this study and other reference strains. PCR and the sequence analysis confirmed canine parvovirus-2a as the etiology of the disease. Good management is advised to avoid secondary or severe dehydration and marked gastrointestinal fluid hypovolemia with loss of protein and bacterial sepsis.
犬细小病毒(CPV)感染被认为是幼犬在6个月以下死亡的常见原因。尽管使用了现有的治疗方法,许多狗仍然死于CPV相关的并发症。本研究旨在描述犬细小病毒感染的临床调查、病理表现和分子诊断。本研究共收集了35个样本,包括血液和粪便样本,来自不同年龄和品种的狗(德国牧羊犬,哈士奇,Loloo和罗威勒幼犬)。研究考虑了犬的病史、重要参数的临床检查、血象改变和粪便检查犬细小病毒抗原的检测。采用CPV-2快速检测试剂盒对10只有犬细小病毒肠炎临床症状的犬直肠拭子进行CPV-2检测。结果9/10拭子CPV-2阳性。为进一步调查,对所有拭子进行分子鉴定,通过提取病毒DNA和常规PCR检测CPV-2,所有样本均呈CPV-2阳性。舌、肺、肾、肠组织病理学检查提示感染CPV肠炎。白细胞减少、中性粒细胞减少、淋巴细胞减少和血小板减少,单核细胞和嗜酸性粒细胞计数显著增加。血清总蛋白和球蛋白显著升高,血清白蛋白显著降低。血清ALT、AST、尿素氮(BUN)、肌酐显著升高。对PCR产物较强的样本进行测序和系统发育分析,以检测本研究CPV与其他参考菌株核苷酸序列的一致性和差异百分比。PCR和序列分析证实犬细小病毒2a是该病的病因。建议采取良好的治疗措施,避免继发性或严重脱水和明显的胃肠道液体低容量血症伴蛋白质丢失和细菌性败血症。
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引用次数: 0
Attempts for controlling of histamine by using bacillus polymyxa in salted fishes 多粘芽孢杆菌控制咸鱼组胺含量的尝试
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.300157
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引用次数: 0
Efficacy of Electrolyzed water in controlling microbial contamination of fresh chicken carcasses 电解水对鲜鸡胴体微生物污染的控制效果
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.300350
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引用次数: 0
Overview on the diagnostic methods of Cryptosporidiosis in bovine calves 犊牛隐孢子虫病诊断方法综述
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.301528
A. Nady
C ryptosporidiosis is one of the most important causes of neonatal enteritis and diarrhea in calves leading to significant morbidity and mortality rates globally. This study was conducted to highlight the diagnostic methods of cryptosporidiosis in pre-weaned cattle calves. For this purpose, a total of 88 diarrheic faecal samples of pre-waned calves at Giza and El-Fayoum governorates, Egypt, were microscopically examined using Modified Ziehl-Neelsen staining (MZN), Immunochromatographic test (ICT) and Enzyme linked Immunosorbent Assay (ELISA). The prevalence of infection was 71.59% (63/88), 73.86% (65/88), and 75.00% (66/88) for MZN, ICT, and ELISA respectively. The Sensitivity was 96.9%, 100%, and 100% whereas; Specificity was 100%, 100%, and 95.7% for MZN, ICT, and ELISA, respectively.
隐孢子虫病是导致新生儿肠炎和犊牛腹泻的最重要原因之一,在全球范围内导致显著的发病率和死亡率。本研究旨在探讨断奶前犊牛隐孢子虫病的诊断方法。为此,使用改良Ziehl-Neelsen染色法(MZN)、免疫层析法(ICT)和酶联免疫吸附法(ELISA)对埃及吉萨省和El-Fayoum省共88头预断奶犊牛的腹泻粪便样本进行了显微镜检查。MZN、ICT和ELISA的感染率分别为71.59%(63/88)、73.86%(65/88)和75.00%(66/88)。灵敏度分别为96.9%、100%和100%,而;MZN、ICT和ELISA的特异性分别为100%、100%和95.7%。
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引用次数: 0
Sheep Brucellosis in Delta Region 三角洲地区绵羊布鲁氏菌病
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.307122
M. El-Diasty, R. El-said, Nour H. Abdel-Hamid
B rucellosis is the generic name for animal and human infections owing to different species of the genus Brucella mainly B. abortus and B. melitensis . B. melitensis was the prevalent serotype among sheep in Delta region, like bovine brucellosis. This paper tailors the epidemiology of brucellosis in small ruminants in Delta region pointing to the evaluation of the ordinarily used diagnostic bacteriological, molecular, and serological procedures. Herein, a broad review to shed light on the complexity of bru-cellosis plus discussing that the test and slaughter strategy should be applicable to free the herd out of quarantine, withal application of biosecurity practices and fair compensation policy for owners for good protection.
布鲁氏菌病是动物和人类感染的总称,因为布鲁氏菌属的不同种类主要是流产布鲁氏菌和melitensis布鲁氏菌。与牛布鲁氏菌病一样,牛利氏菌是三角洲地区绵羊流行的血清型。本文针对三角洲地区小反刍动物布鲁氏菌病的流行病学进行了调整,指出了对常用的细菌学、分子和血清学诊断程序的评估。本文对布鲁氏菌病的复杂性进行了广泛的回顾,并讨论了应适用检测和屠宰策略,以使畜群免于检疫,同时应用生物安全措施和为业主提供良好保护的公平补偿政策。
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引用次数: 0
A meta-analysis of the Impact of Endometritis on Reproductive and Productive Performances of Lactating Dairy Herds 子宫内膜炎对哺乳期奶牛繁殖和生产性能影响的荟萃分析
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.302584
M. Kandiel
E ndometritis is a major postpartum disease that affects the productive and reproductive performance of dairy cows that is accompanied by heavy economic losses to the farmer. We conduct a meta-analysis to evaluate the effect of Endometritis on daily milk yield (DMY) and some reproductive indices (pregnancy rate, days open (DO), service per conception (S/C), and days to the first insemination) in lactating dairy cows. Data corresponding to 4037 cows was extracted from eleven research papers with suf-ficient data for analysis. The odds ratio was calculated for binary data (Endometritis incidence and pregnancy rate) by using the multinomial logistic regression, while the summary estimate was calculated for continuous data (DMY, S/C, and DO) by using the Linear mixed model (Multilevel regression). The odds ratio (OR) of Endometritis had a significant difference among different studies and years, paper No.4 recorded an equal number of diseased and healthy cows (OR=1), so we take this paper as a reference category, OR was the highest (1.6) for paper No.10, while it was the lowest (0.2) for paper No. 2. Concerning the pregnancy rate, Endometritis significantly reduced the pregnancy rate by 45% compared with the healthy cow. Regarding the DMY, Endometritis had a significant reduction in DMY by 1.7 kg rather than the healthy one. Concerning the DO, the cow that suffered from Endometritis had a more prolonged DO by 58 days than the healthy one, also S/C had a significant increase by 1.6 compared with healthy cows. Finally, we concluded that Endometritis had an adverse effect on the
子宫炎是一种影响奶牛生产和繁殖性能的主要产后疾病,并给养殖户带来严重的经济损失。为了评价子宫内膜炎对哺乳期奶牛日产奶量(DMY)和一些生殖指标(妊娠率、开胎天数(DO)、单胎服务时间(S/C)和首次授精天数)的影响,我们进行了一项荟萃分析。4037头奶牛的数据从11篇研究论文中提取,有足够的数据进行分析。二元数据(子宫内膜炎发病率和妊娠率)采用多项logistic回归计算比值比,连续数据(DMY、S/C和DO)采用线性混合模型(multi - level regression)计算汇总估计。子宫内膜炎的比值比(OR)在不同的研究和年份之间存在显著差异,4号论文记录的患病奶牛和健康奶牛数量相等(OR=1),因此我们将这篇论文作为参考类别,10号论文OR最高(1.6),2号论文OR最低(0.2)。在妊娠率方面,子宫内膜炎与健康奶牛相比,妊娠率显著降低45%。在DMY方面,子宫内膜炎患者的DMY比健康组明显减少1.7 kg。在DO方面,子宫内膜炎奶牛的DO比健康奶牛延长了58天,S/C比健康奶牛显著提高了1.6天。最后,我们得出结论,子宫内膜炎有不良影响
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引用次数: 0
Detection and Molecular Characterization of Avian Reovirus (ARV) Variants in Egypt with studying the effect of Selenium Nanoparticles on Growth Performance after Experimental ARV challenge in broilers. 禽呼肠孤病毒(ARV)在埃及的检测和分子特征及纳米硒对ARV攻毒后肉鸡生长性能的影响
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.302141
Mahmoud S. Sabra, EidAli Hussein, N. F. Anwar, Shen El, Sebaey, W. Salem, Dina M. W. Shibat Elhamd
.
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引用次数: 0
Comparison between heavy metal residues and their effect on African Catfish (Clarias gariepinus) and Nile Tilapia (Oreochromis niloticus) 非洲鲶鱼(Clarias gariepinus)和尼罗罗非鱼(Oreochromis niloticus)重金属残留量及其影响比较
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.305223
D. Mohamed, Maha M. El Alem, M. Ibrahim, Thoria A. Hamed
: F ish are an important source of proteins of a high value. Bioaccumulation of heavy metals in aquatic animals causes serious threat for fish as well as human via persistent consumption of fish. This study aims to estimate the concentrations of some heavy metals (lead (Pb), cadmium (Cd) and chromium (Cr)) in fish muscles and water. Samples of fish muscles ( Clariasgariepinus and Oreochromisniloticus ) and water were collected from different farms in Sharkia province in Egypt from April to September in 2022. The concentrations of heavy metals had the order Pb> Cr > Cd. The highest values of metals were recorded for Pb in the examined fish while the lowest values were recorded for Cd and Cr. There is a variation in the concentrations of heavy metals in water between investigated farms. The highest values of metals were recorded for Pb in the water. a significant decrease in RBCs count, Hb conc., PCV% and WBCs compared with control group with significant increase in MCV, MCH values in farm B, C and D compared to control and the most pronounced significant changes was correlated to farm C. A significant decrease in phagocytic index and percentage were the recorded effect of heavy metals. The impact of heavy metals on biochemical parameters showed significant increase of AST, ALT, urea, creatinine, glucose and total lipids in the blood of examined fish, but total protein and total globulin, beta and gamma globulin revealed a significant decrease. They
鱼是高价值蛋白质的重要来源。水生动物体内重金属的生物积累通过持续食用鱼类对鱼类和人类造成严重威胁。本研究旨在估计鱼类肌肉和水中某些重金属(铅(Pb)、镉(Cd)和铬(Cr))的浓度。于2022年4月至9月在埃及Sharkia省的不同养殖场收集了鱼肌肉(Clariasgariepinus和Oreochromisniloticus)和水样本。重金属含量的顺序为Pb> Cr > Cd,受测鱼类中Pb的含量最高,Cd和Cr的含量最低。不同养殖场水体中重金属含量存在差异。铅在水中的金属含量最高。红细胞计数显著下降,血红蛋白含量显著下降。MCV、MCH值在B场、C场和D场均较对照组显著升高,且与C场相关的变化最为显著,记录的吞噬指数和吞噬百分比均显著降低。重金属对血液生化指标的影响表现为AST、ALT、尿素、肌酐、葡萄糖和总脂质的显著升高,而总蛋白和总球蛋白、β和γ球蛋白的显著降低。他们
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引用次数: 0
Pathogenicity of bacterial isolates associated with high mortality in duckling in Behira province 贝拉省与小鸭高死亡率相关的细菌分离株的致病性
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.302096
E. Yonis
D uring 2020–2022, a survey of 25 farms of ducklings of various breeds (Pekini and Muscovy), that ranged from one day up to a month of age suffering from various symptoms and mortality rates varied from 10 to 35 % revealed isolation of Klebsiella pneumonae 36 % whereas the prevalence of E coli, Staph aureus and Salmonella Typhimurium were 32%, 28% and 16% respectively. Following PCR confirmation of Klebsiella pneumonae, the presence of the its virulence genes were sur-veyed and results showed that uge (44%) and rmpA (22%), as well as an antimicrobial susceptibility profile , which revealed 100% resistant to Ampicillin, Amoxicillin and Penicillin G followed by77 % toward tetracycline, 22% toward streptomycin . Contrarily, they were extremely sensitive to ciprofloxacin and enrofloxacin, as well as 77% to cefotaxime and 66% to gentamycin. The surveillance of class 1 integron ( intl1) and genes of antimicrobial resistance demonstrated that (66%) of isolates harbor Int1,whereas only 33% of the isolates involved blaSHV, they all had the tetA and blaTEM genes. Salmonella and E. coli isolates were serotyped, and it was discovered that the E. coli isolates belonged to six different O-serogroups, including O55:H7, O148:K25, O86: K61, O114:H21, O26:H11 and O127:H6
D在2020-2022年期间,对25个不同品种(北京和莫斯科)雏鸭养殖场的调查显示,肺炎克雷伯菌的分离率为36%,大肠杆菌、金黄色葡萄球菌和鼠伤寒沙门氏菌的流行率分别为32%、28%和16%,雏鸭的年龄从一天到一个月不等,出现各种症状,死亡率从10%到35%不等。对肺炎克雷伯菌进行PCR鉴定后,调查其毒力基因的存在,结果显示uge(44%)和rmpA(22%),以及抗菌药物敏感性谱,结果显示对氨苄西林、阿莫西林和青霉素G耐药100%,对四环素耐药77%,对链霉素耐药22%。相反,他们对环丙沙星和恩诺沙星极其敏感,对头孢噻肟和庆大霉素分别为77%和66%。1类整合子(Int1)和耐药基因检测结果显示,66%的分离株含有Int1,而仅有33%的分离株含有blaSHV,它们均含有tetA和blaTEM基因。对分离的沙门氏菌和大肠杆菌进行血清分型,发现分离的大肠杆菌分别属于O55:H7、O148:K25、O86: K61、O114:H21、O26:H11和O127:H6 6个不同的o血清群
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引用次数: 0
Application Of Artificial Intelligence In The Management Of Poultry Farms And Combating Antimicrobial Resistance 人工智能在家禽养殖场管理和抗微生物药物耐药性中的应用
Pub Date : 2023-07-01 DOI: 10.21608/ejah.2023.302769
Reham A. Hosny, N. Alatfeehy, M. Abdelaty
A ntimicrobial resistance (AMR) is currently one of the most danger-ous crises facing the world. There has been an unsettling rise in the antimicrobial resistance (AMR) identified in animals, which may transfer to people through direct contact, environmental pollution, and food consumption. Efficient poultry health and welfare management and quick diagnosis of bacterial infections in poultry farms can lessen the demand for antibiotics, which is reflected on the spreading of epidemics and AMR. Internet of thinking and machine learning are branches of Artificial intelligence that enable intelligent autonomous systems with human workers remotely managing operations. Machine learning technology plays a role in tracking and preventing infections in poultry farms, which can reduce the need for antibiotic treatment in poultry and, as a result, limit the transmission of antibiotic-resistant pathogens to humans. This information is further analyzed by powerful processing computers with the aid of massive storage devices to seek for trends and hints to pinpoint the locations of disease outbreaks and cases of resistance. The utilization of this knowledge will make it easier to prevent epidemics in the future, reducing the demand for antibiotics. Therefore, this review offers insight on the current AI practices in the management of poultry farms as well as opportunities and concerns around antibiotic resistance throughout the world.
抗生素耐药性(AMR)是目前世界面临的最危险的危机之一。在动物中发现的抗菌素耐药性(AMR)出现了令人不安的上升,这种耐药性可能通过直接接触、环境污染和食物消费转移给人类。有效的家禽健康和福利管理以及家禽养殖场细菌感染的快速诊断可以减少对抗生素的需求,这反映在流行病和抗生素耐药性的传播上。思维互联网和机器学习是人工智能的分支,它们使智能自主系统与人类工人远程管理操作成为可能。机器学习技术在跟踪和预防家禽养殖场感染方面发挥着重要作用,这可以减少家禽对抗生素治疗的需求,从而限制耐抗生素病原体向人类的传播。这些信息由强大的处理计算机在海量存储设备的帮助下进一步分析,以寻找趋势和线索,以查明疾病爆发和耐药性病例的位置。利用这些知识将使将来预防流行病变得更加容易,从而减少对抗生素的需求。因此,这篇综述对目前家禽养殖场管理中的人工智能实践以及世界各地抗生素耐药性的机遇和担忧提供了见解。
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引用次数: 0
期刊
Egyptian Journal of Animal Health
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