Pub Date : 2022-10-01DOI: 10.21608/ejah.2022.284061
{"title":"Investigation on some bacterial and fungal causes of buffaloes calves diarrhea with emphasis on suitable treatment","authors":"","doi":"10.21608/ejah.2022.284061","DOIUrl":"https://doi.org/10.21608/ejah.2022.284061","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91545646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/ejah.2022.264911
Huda Elsayed, Nashwa M. Zaki, Yosra Samy Aleslamboly
E lectrolyzed Water is a potent multifunctional antibacterial agent with numerous applications in food safety via processing, storage as well as it has great importance in agriculture and medicinal applications. The current study investigate the powerful of the sanitizing effect of slightly acidic (SAcEW) as well as the detergent effect of slightly alkaline electrolyzed water (SAlEW) (individually and in combination) on chilled chicken fillets subjected to a dipping treatment for 5 min and stored refrigerated at 4±1°C. When treated were compared to untreated samples, it was turned out to be that SAlEW and SAcEW treatments increased the shelf life of chilled chicken fillet and remained fit for consumption till 7th and 9th days, recorded APC 4.89±0.01 and 4.9±0.0, psychrophilic plate count 3.8±0.02 and 2.18±0.03, E. coli count 1.97±0.03 and 1.98±0.02 and Molds count 3.84±0.02 and 3.56±0.68, respectively. Moreover, dipping of chicken fillet in SAlEW followed by SAcEW resulted in prolong the shelf-life until the 11th day of storage, recorded APC 4.94±0.01, psychrophilic plate count 4.67±0.02, E. coli count 1.98±0.03 and Molds count 3.88±0.03.
{"title":"Impact of slightly acidic and alkaline electrolyzed water on shelf-life of the chilled chicken fillet","authors":"Huda Elsayed, Nashwa M. Zaki, Yosra Samy Aleslamboly","doi":"10.21608/ejah.2022.264911","DOIUrl":"https://doi.org/10.21608/ejah.2022.264911","url":null,"abstract":"E lectrolyzed Water is a potent multifunctional antibacterial agent with numerous applications in food safety via processing, storage as well as it has great importance in agriculture and medicinal applications. The current study investigate the powerful of the sanitizing effect of slightly acidic (SAcEW) as well as the detergent effect of slightly alkaline electrolyzed water (SAlEW) (individually and in combination) on chilled chicken fillets subjected to a dipping treatment for 5 min and stored refrigerated at 4±1°C. When treated were compared to untreated samples, it was turned out to be that SAlEW and SAcEW treatments increased the shelf life of chilled chicken fillet and remained fit for consumption till 7th and 9th days, recorded APC 4.89±0.01 and 4.9±0.0, psychrophilic plate count 3.8±0.02 and 2.18±0.03, E. coli count 1.97±0.03 and 1.98±0.02 and Molds count 3.84±0.02 and 3.56±0.68, respectively. Moreover, dipping of chicken fillet in SAlEW followed by SAcEW resulted in prolong the shelf-life until the 11th day of storage, recorded APC 4.94±0.01, psychrophilic plate count 4.67±0.02, E. coli count 1.98±0.03 and Molds count 3.88±0.03.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84432983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/ejah.2022.286330
{"title":"Detection of Enterotoxin A gene in Staphylococcus aureus isolated from Broiler farms in Bohera Governorate","authors":"","doi":"10.21608/ejah.2022.286330","DOIUrl":"https://doi.org/10.21608/ejah.2022.286330","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76849559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/ejah.2022.273718
S. Nagati, Hammad O. Hammad, N. Khalil, Fayza A. Sdeek, Essam, Kamel, Hanaa A. Mabrouk, M. Shahein
T hree cases of buffaloes and their calves were exposed to acute toxicity after feeding on sprayed alfalfa fodder by chlorpyrifos at Monshaat El-Gammal, Tamiyyah city, El-Fayoum Governorate, Egypt. On treatment of animals with atropine sulphate, adult animals were recovered; two of their calves were recovered within 1 hour, while last one dead. The content of used pesticide active ingredient “chlorpyrifos” and its relevant impurity sulfotep in commercial pesticide formulations (480 g a. i. /L) were estimated by gas chromatography-flame ionization detector (GC-FID) against external standard of high purity. Gas chromatog-raphymass spectrometry method was used for the qualitative and quantitative anal-yses of chlorpyrifos in the formulated sample and in animal serum, diarrhoeic material as well as milk of exposed animals. The results of analysis showed that the content of chlorpyrifos in the sample is 214.15 g/L which is not comply with the Food and Agricultural Organization (FAO) specifications for the active substance (chlorpyrifos). The content of the relevant impurity sulfotep was estimated as 0.269 % of chlorpyrifos content which comply and close to the corresponding maximum permitted level 3 g/kg of the chlorpyrifos content found. GC-MSMS MRM for chlorpyrifos was 350 m/w for Precursor Ion and Fragment ions were 97, 199 m/z. The recovery percent of chlorpyrifos ranged between 71.8 and 94.65% with relative standard deviation (RSD) below 1.45%. Average calculated residues of chlorpyrifos were 2.6 ppm in milk, 80.8 ppb in diarrheic materials and 37.4 ppm in grass (alfalfa fodder). This disturbance create suitable media for some pathogenic bacteria invasion, Nine bacterial isolates (4 E. coli, 2 Staph. aureus, 2 Coliform and 1 Klebsiella ) from milk, saliva, nasal and faecal samples of 3 buffaloes and 3 buffalo's calves suffering from severe diarrhoea. In conclusion: This study proved that exposure to chlorpyrifos induced disturbance in the intestinal microflora with stim-ulated pathogenic bacteria causing enteritis. While true used chlorpyrifos pesticiede did not agree with bottle contents. So, more control over pesticides application is required in villages and Ezabs at rural areas.
在埃及El-Fayoum省Tamiyyah市Monshaat El-Gammal，有3例水牛及其幼崽在食用喷洒毒死蜱的苜蓿饲料后暴露于急性毒性。用硫酸阿托品治疗动物，成年动物恢复;其中两只幼崽在一小时内被发现，最后一只死亡。采用气相色谱-火焰离子化检测器(GC-FID)对市售农药制剂中已用农药有效成分毒死蜱及其相关杂质硫步的含量(480 g a. i. /L)进行了测定。采用气相色谱-质谱法对配制样品和动物血清、腹泻物质以及接触动物的乳汁中的毒死蜱进行定性和定量分析。分析结果表明，样品中毒死蜱的含量为214.15 g/L，不符合联合国粮农组织(FAO)对活性物质毒死蜱的规定。相关杂质硫步的含量估计为毒死蜱含量的0.269%，符合并接近毒死蜱含量的相应最大允许水平3 g/kg。毒死蜱前体离子和碎片离子的GC-MSMS MRM分别为350 m/w和97、199 m/z。毒死蜱的回收率在71.8 ~ 94.65%之间，相对标准偏差(RSD)小于1.45%。毒死蜱在牛奶中的平均计算残留量为2.6 ppm，在腹泻材料中为80.8 ppb，在草(苜蓿饲料)中为37.4 ppm。这种干扰为某些致病菌的入侵创造了适宜的培养基，分离出9株细菌(大肠杆菌4株，葡萄球菌2株)。从患有严重腹泻的3头水牛和3头水牛小牛的牛奶、唾液、鼻腔和粪便样本中发现了金黄色葡萄球菌、2种大肠菌群和1种克雷伯菌)。结论:本研究证明，毒死蜱暴露可引起肠道菌群紊乱，刺激致病菌引起肠炎。而实际使用的毒死蜱农药与瓶子内容不符。因此，需要在农村和农村地区加强对农药使用的控制。
{"title":"Effect of spraying chlorpyrifos on green weeds and its relationship to bacterial enteritis in calves: Case study","authors":"S. Nagati, Hammad O. Hammad, N. Khalil, Fayza A. Sdeek, Essam, Kamel, Hanaa A. Mabrouk, M. Shahein","doi":"10.21608/ejah.2022.273718","DOIUrl":"https://doi.org/10.21608/ejah.2022.273718","url":null,"abstract":"T hree cases of buffaloes and their calves were exposed to acute toxicity after feeding on sprayed alfalfa fodder by chlorpyrifos at Monshaat El-Gammal, Tamiyyah city, El-Fayoum Governorate, Egypt. On treatment of animals with atropine sulphate, adult animals were recovered; two of their calves were recovered within 1 hour, while last one dead. The content of used pesticide active ingredient “chlorpyrifos” and its relevant impurity sulfotep in commercial pesticide formulations (480 g a. i. /L) were estimated by gas chromatography-flame ionization detector (GC-FID) against external standard of high purity. Gas chromatog-raphymass spectrometry method was used for the qualitative and quantitative anal-yses of chlorpyrifos in the formulated sample and in animal serum, diarrhoeic material as well as milk of exposed animals. The results of analysis showed that the content of chlorpyrifos in the sample is 214.15 g/L which is not comply with the Food and Agricultural Organization (FAO) specifications for the active substance (chlorpyrifos). The content of the relevant impurity sulfotep was estimated as 0.269 % of chlorpyrifos content which comply and close to the corresponding maximum permitted level 3 g/kg of the chlorpyrifos content found. GC-MSMS MRM for chlorpyrifos was 350 m/w for Precursor Ion and Fragment ions were 97, 199 m/z. The recovery percent of chlorpyrifos ranged between 71.8 and 94.65% with relative standard deviation (RSD) below 1.45%. Average calculated residues of chlorpyrifos were 2.6 ppm in milk, 80.8 ppb in diarrheic materials and 37.4 ppm in grass (alfalfa fodder). This disturbance create suitable media for some pathogenic bacteria invasion, Nine bacterial isolates (4 E. coli, 2 Staph. aureus, 2 Coliform and 1 Klebsiella ) from milk, saliva, nasal and faecal samples of 3 buffaloes and 3 buffalo's calves suffering from severe diarrhoea. In conclusion: This study proved that exposure to chlorpyrifos induced disturbance in the intestinal microflora with stim-ulated pathogenic bacteria causing enteritis. While true used chlorpyrifos pesticiede did not agree with bottle contents. So, more control over pesticides application is required in villages and Ezabs at rural areas.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80465596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-01DOI: 10.21608/ejah.2022.283457
{"title":"Highlighting on Bovine Tuberculosis infection in cattle","authors":"","doi":"10.21608/ejah.2022.283457","DOIUrl":"https://doi.org/10.21608/ejah.2022.283457","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83632704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.21608/ejah.2022.251392
M. Fadel, H. Badr
T his study was performed to investigate the liposome coated colistin for enhancing the oral pharmacokinetics, bioavailability, delivery and efficacy of the drug against resistant E. coli strain. Minimum inhibitory concentration (MIC) of colistin (C) and liposomal colistin (L) was 1.56 µg/ml and 0.00156 µg/ml for O125 sensitive colistin strain (CS and LS), while 100µg/ml and 0.025 µg/ml for O125 resistant strain (CR and LR). In vitro, time kill kinetics of liposomal colistin against sensitive and resistant O125 recorded 100% and 82.8% as a reduction% at 1 MIC, while ≥99.9% at 2 MIC for both strains after 1hr incubation time. The pharmacokinetic/ pharmacodynamics profiles were studied by single oral dose of colistin and liposomal colistin at 100000 IU/kg b.wt in healthy and diseased chicken. The pharmacokinetic parameters; Cmax/MIC ratio for CS and CR were 3.5 and 0.06. While, liposomal colistin recorded 3.9x10 3 and 247.2 for LS and LR, respectively. AUC/MIC ratios were 13.4, 0.248, 40.9x10 3 and 2.6x10 3 for CS, CR, LS and LR, respectively; proving the high efficacy of liposomal colistin with less significant activity of colistin. There was significant increment of t 1/2 Beta and MRT of liposomal colistin groups in comparison with colistin groups. Contraries, clearance Time (CL/F) was significantly decreased in liposomal colistin than colistin groups. Liposomal colistin en-hanced the bioavailability% from 5.2% to 49.2%. Liver E. coli count re-vealed highly significant decrease of LC for both strains nearly similar to negative control group after repeated treatment for 5 consecutive days; indicating the great effect by liposomal colistin on both E. coli strains especially for colistin resistant strain. This study recommends the liposomal colistin formulation against multidrug resistant E. coli infections.
{"title":"Antimicrobial activity of Liposomal colistin against resistant E. coli in vitro and in vivo","authors":"M. Fadel, H. Badr","doi":"10.21608/ejah.2022.251392","DOIUrl":"https://doi.org/10.21608/ejah.2022.251392","url":null,"abstract":"T his study was performed to investigate the liposome coated colistin for enhancing the oral pharmacokinetics, bioavailability, delivery and efficacy of the drug against resistant E. coli strain. Minimum inhibitory concentration (MIC) of colistin (C) and liposomal colistin (L) was 1.56 µg/ml and 0.00156 µg/ml for O125 sensitive colistin strain (CS and LS), while 100µg/ml and 0.025 µg/ml for O125 resistant strain (CR and LR). In vitro, time kill kinetics of liposomal colistin against sensitive and resistant O125 recorded 100% and 82.8% as a reduction% at 1 MIC, while ≥99.9% at 2 MIC for both strains after 1hr incubation time. The pharmacokinetic/ pharmacodynamics profiles were studied by single oral dose of colistin and liposomal colistin at 100000 IU/kg b.wt in healthy and diseased chicken. The pharmacokinetic parameters; Cmax/MIC ratio for CS and CR were 3.5 and 0.06. While, liposomal colistin recorded 3.9x10 3 and 247.2 for LS and LR, respectively. AUC/MIC ratios were 13.4, 0.248, 40.9x10 3 and 2.6x10 3 for CS, CR, LS and LR, respectively; proving the high efficacy of liposomal colistin with less significant activity of colistin. There was significant increment of t 1/2 Beta and MRT of liposomal colistin groups in comparison with colistin groups. Contraries, clearance Time (CL/F) was significantly decreased in liposomal colistin than colistin groups. Liposomal colistin en-hanced the bioavailability% from 5.2% to 49.2%. Liver E. coli count re-vealed highly significant decrease of LC for both strains nearly similar to negative control group after repeated treatment for 5 consecutive days; indicating the great effect by liposomal colistin on both E. coli strains especially for colistin resistant strain. This study recommends the liposomal colistin formulation against multidrug resistant E. coli infections.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75088225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.21608/ejah.2022.253864
{"title":"Microbial and Pathological Investigation of Lambs Deaths in Sheep Flock at Hosh-Issa , El- Bohaira Governorate","authors":"","doi":"10.21608/ejah.2022.253864","DOIUrl":"https://doi.org/10.21608/ejah.2022.253864","url":null,"abstract":"","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84444221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.21608/ejah.2022.252843
M. Saleh, M. Rateb, E. Abd-Allah, O. M. Mahran, G. Mohamed
ipetalonema evansi ( D. evansi ) is an important filarial nematode especially in camels and represents a significant health problem. This study aimed to determine the effect of natural infection with D. evansi on the oxidative status in plasma and erythrocytes of camels ( Camelus dromedarius ). In this study, 22 female dromedaries were classi-fied according to blood parasitological examinations into infected group (n = 10) and healthy control one (n = 12). Compared to values of the control group, a significant reduction in red blood cell count ( P < 0.05), hemoglobin concentration ( P < 0.01) and packed cell volume ( P < 0.01) was noticed in the infected camels. Plasma total oxidant status (TOS) was significantly increased ( P < 0.05) in the infected camel group compared to the control one. Plasma total antioxidant capacity (TAC) was significantly decreased ( P < 0.05) whereas the erythrocytic activity of the antioxidant superoxide dismutase and erythrocytic lipid peroxidation as denoted by malondialdehyde concentration were increased ( P < 0.05) in the infected group when compared to the corresponding control values. In conclusion, erythrocyte of camels infected by D. evansi is a subject of peroxidation,
{"title":"Corpuscular oxidation in camels naturally infected with Dipetalonema evansi","authors":"M. Saleh, M. Rateb, E. Abd-Allah, O. M. Mahran, G. Mohamed","doi":"10.21608/ejah.2022.252843","DOIUrl":"https://doi.org/10.21608/ejah.2022.252843","url":null,"abstract":"ipetalonema evansi ( D. evansi ) is an important filarial nematode especially in camels and represents a significant health problem. This study aimed to determine the effect of natural infection with D. evansi on the oxidative status in plasma and erythrocytes of camels ( Camelus dromedarius ). In this study, 22 female dromedaries were classi-fied according to blood parasitological examinations into infected group (n = 10) and healthy control one (n = 12). Compared to values of the control group, a significant reduction in red blood cell count ( P < 0.05), hemoglobin concentration ( P < 0.01) and packed cell volume ( P < 0.01) was noticed in the infected camels. Plasma total oxidant status (TOS) was significantly increased ( P < 0.05) in the infected camel group compared to the control one. Plasma total antioxidant capacity (TAC) was significantly decreased ( P < 0.05) whereas the erythrocytic activity of the antioxidant superoxide dismutase and erythrocytic lipid peroxidation as denoted by malondialdehyde concentration were increased ( P < 0.05) in the infected group when compared to the corresponding control values. In conclusion, erythrocyte of camels infected by D. evansi is a subject of peroxidation,","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80704880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-01DOI: 10.21608/ejah.2022.252840
M. Saleh, M. Rateb, E. Abd-Allah, G. Mohamed
D ermatophyte infection caused by Trichophyton verrucosum has a substantial impact on animal and human health. The present work aimed to determine the reactive oxygen metabolites (ROM), total antioxidant capacity (TAC), lipid peroxides (indicated by malondialdehyde, MDA) and measuring the oxidative stress index (OSI), in addition to investigation of some hematological and trace elements in sheep with dermatophytosis. T. verrucosum was diagnosed clinically and mycologically in 12 young sheep with mild infection (1-2 lesions, each with an approximate diameter of 2-4 cm) and 9 with severe lesions (covering more than a quarter of the head and ear), as well as 12 age- and gender-matched healthy control individuals. In comparison to controls, red blood cell count, packed cell volume and hemoglobin did not differ (P > 0.05) in the infected group, whereas leukocytic count increased (P < 0.05) in the severely infected group accompanied by neutrophilia, monocytosis and eosinophilia. Plasma Zn decreased (P < 0.05) in the severely infected group but Cu did not differ (P > 0.05) between groups. There was a progressive increase in the ROM in mild (P < 0.05) and severely infected group (P < 0.01) than the controls. The SOD activity and TAC were higher (P < 0.05) in the mild infected group, and lower in the severely infected group (P < 0.05). MDA and OSI did not change (P > 0.05) in the mild, but increased (P < 0.01) in the severely infected group. As a conclusion, sheep with severe infections by T. verrucosum exhibits disturbance in the leukogram, and Zn deficiency, associated with enhanced prooxidants and impaired antioxidant potential, suggesting an oxidative stress status.
{"title":"Circulating redox status in sheep infected with dermatophytosis: a field study","authors":"M. Saleh, M. Rateb, E. Abd-Allah, G. Mohamed","doi":"10.21608/ejah.2022.252840","DOIUrl":"https://doi.org/10.21608/ejah.2022.252840","url":null,"abstract":"D ermatophyte infection caused by Trichophyton verrucosum has a substantial impact on animal and human health. The present work aimed to determine the reactive oxygen metabolites (ROM), total antioxidant capacity (TAC), lipid peroxides (indicated by malondialdehyde, MDA) and measuring the oxidative stress index (OSI), in addition to investigation of some hematological and trace elements in sheep with dermatophytosis. T. verrucosum was diagnosed clinically and mycologically in 12 young sheep with mild infection (1-2 lesions, each with an approximate diameter of 2-4 cm) and 9 with severe lesions (covering more than a quarter of the head and ear), as well as 12 age- and gender-matched healthy control individuals. In comparison to controls, red blood cell count, packed cell volume and hemoglobin did not differ (P > 0.05) in the infected group, whereas leukocytic count increased (P < 0.05) in the severely infected group accompanied by neutrophilia, monocytosis and eosinophilia. Plasma Zn decreased (P < 0.05) in the severely infected group but Cu did not differ (P > 0.05) between groups. There was a progressive increase in the ROM in mild (P < 0.05) and severely infected group (P < 0.01) than the controls. The SOD activity and TAC were higher (P < 0.05) in the mild infected group, and lower in the severely infected group (P < 0.05). MDA and OSI did not change (P > 0.05) in the mild, but increased (P < 0.01) in the severely infected group. As a conclusion, sheep with severe infections by T. verrucosum exhibits disturbance in the leukogram, and Zn deficiency, associated with enhanced prooxidants and impaired antioxidant potential, suggesting an oxidative stress status.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78844101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.21608/ejah.2022.252260
Manal M. Said, Taghreed H. Abbas
I n the current study, migration of heavy metals represent by lead (pb) and cadmium (Cd) were investigated before and after cooking using different cooking made pots (ceramic, glass and granite). Fifteen raw beef samples were tested for the presence of both two elements residues before cooking. Lead was observed in all raw meat samples out of which,11 samples (73.3%) were accepted and 4 samples (26.7%) were not-accepted. On the other side, cadmium was mostly not detected (lower than 0.01 mg/kg) in all tested raw according Standers Three of tested beef samples that had lower content of both elements were divided into three portions to be cooked in ceramic, glass and granite cooking pots. Lead noticed to have migrated level from ceramic pots exceeding the acceptable limit (0.1 mg/kg) with a mean value of 0.32±0.21 mg/kg followed by granite which recorded 0.093±0.01mg/kg and lastly for glass pots with mean of 0.072±0.005mg/kg, while cadmium was within the acceptable limit for all tested samples after cooking(0.05 mg/kg) except for one sample which was cooked in ceramic pot with 33.3% not accepted. This study demonstrated that ceramic pots was the most leachable of lead followed by granite and lastly glass pots. The current study supported the need to use a good quality material in cooking pots manufacturing.
{"title":"Detection of lead and cadmium residues in beef before and after cooking in ceramic, glass and granite pots","authors":"Manal M. Said, Taghreed H. Abbas","doi":"10.21608/ejah.2022.252260","DOIUrl":"https://doi.org/10.21608/ejah.2022.252260","url":null,"abstract":"I n the current study, migration of heavy metals represent by lead (pb) and cadmium (Cd) were investigated before and after cooking using different cooking made pots (ceramic, glass and granite). Fifteen raw beef samples were tested for the presence of both two elements residues before cooking. Lead was observed in all raw meat samples out of which,11 samples (73.3%) were accepted and 4 samples (26.7%) were not-accepted. On the other side, cadmium was mostly not detected (lower than 0.01 mg/kg) in all tested raw according Standers Three of tested beef samples that had lower content of both elements were divided into three portions to be cooked in ceramic, glass and granite cooking pots. Lead noticed to have migrated level from ceramic pots exceeding the acceptable limit (0.1 mg/kg) with a mean value of 0.32±0.21 mg/kg followed by granite which recorded 0.093±0.01mg/kg and lastly for glass pots with mean of 0.072±0.005mg/kg, while cadmium was within the acceptable limit for all tested samples after cooking(0.05 mg/kg) except for one sample which was cooked in ceramic pot with 33.3% not accepted. This study demonstrated that ceramic pots was the most leachable of lead followed by granite and lastly glass pots. The current study supported the need to use a good quality material in cooking pots manufacturing.","PeriodicalId":11415,"journal":{"name":"Egyptian Journal of Animal Health","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84149225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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