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Synergistic anti-microbial effect of grilling and edible coating on spore forming B. cereus in beef fillets 烧烤和食用涂层对牛肉片中芽孢杆菌孢子形成的协同抗微生物作用
Pub Date : 2024-01-01 DOI: 10.21608/ejah.2024.337817
Nahla A. Abo EL-Roos, Asmaa A. Hashhash, Mohebat A. Abd El-Aziz, El Sayed, Abd El
Q uality and safety of refrigerated beef fillet represented a concern for consumers. Moreover, synergistic effect between two or more of anti-microbial protocols to control bacteria in food especially heat resistant spore forming bacteria is a demand for food safety. So, aim of this study was to evaluate the synergistic application of grilling (heat treatment), and CMC coated samples supported by lactoferrin and propolis on survival of Bacillus cereus in beef fillets. Results revealed an effective synergistic antimicrobial effect of LF and PR incorporated into the CMC edible coating in a combination with grilling against experimentally inoculated B. cereus. So, grilling alone as antimicrobial can't eliminate B. cereus in beef fillets, while application of edible coating fortified with LF and PR enhanced the antimicrobial effect of grilling in a synergistic way to control B. cereus .
冷藏牛肉片的质量和安全是消费者关心的问题。此外,两种或多种抗微生物方案之间的协同效应可控制食品中的细菌,尤其是耐热孢子形成菌,这也是食品安全的要求。因此,本研究旨在评估烧烤(热处理)与乳铁蛋白和蜂胶支持的 CMC 涂层样品对牛肉片中蜡样芽孢杆菌存活的协同作用。结果表明,在 CMC 可食用涂层中加入乳铁蛋白和蜂胶,并与烧烤结合使用,对实验接种的蜡样芽孢杆菌具有有效的协同抗菌效果。因此,仅靠烧烤作为抗菌剂并不能消灭牛柳中的蜡样芽孢杆菌,而添加了 LF 和 PR 的可食用涂层则以协同增效的方式增强了烧烤的抗菌效果,从而控制了蜡样芽孢杆菌。
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引用次数: 0
Beneficial Application of Electrolyzed Water in Food Industry 电解水在食品工业中的有益应用
Pub Date : 2024-01-01 DOI: 10.21608/ejah.2024.321173
Huda El-Sayed
E lectrolyzed water (EW) is a novel technology that has arisen in recent years with potential applications in foods, mostly in microbiological features, with variations in application modalities, dipping the item in solution, where duration may be varied, and being applied in the form of spray. Additionally, EW is a potent sanitizing tool that has received a lot of attention in the food business worldwide. Due to the EW properties, its activity on microorganisms is still being explored for mechanism elucidation and potential harm, as well as its influence on intrinsic food features like as color and oxidation. This unconventional or 'green' technology aims to demonstrate the microbiological contamination control of food while exclude or not rely on the use of chemical dis-infectants as chlorinated water which may result in producing little chemical/toxic residues. This review covered the use of EW in animal-derived foods, emphasizing its safety level, shelf life, and processing.
溶解水(EW)是近年来出现的一种新型技术,在食品中具有潜在的应用价值,主要用于微生物特性方面,其应用方式多种多样,可将物品浸入溶液中,持续时间可以改变,也可采用喷洒的形式。此外,EW 是一种有效的消毒工具,在全球食品行业受到广泛关注。由于 EW 的特性,人们仍在探索它对微生物的作用机制和潜在危害,以及它对食品固有特征(如颜色和氧化)的影响。这种非传统或 "绿色 "技术旨在展示食品微生物污染控制,同时排除或不依赖使用化学消毒剂(如氯化水),因为氯化水可能会产生少量化学/有毒残留物。本综述介绍了在动物源性食品中使用 EW 的情况,强调了其安全水平、保质期和加工过程。
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引用次数: 0
Seroprevalence of Ovine and Caprine brucellosis and associated risk factors in Dakahlia and Damietta Governorates 达卡利亚省和达米埃塔省绵羊和山羊布鲁氏菌病血清流行率及相关风险因素
Pub Date : 2024-01-01 DOI: 10.21608/ejah.2024.321747
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引用次数: 0
Persistent Circulation of Very virulent Infectious Bursal Disease virus (IBDV) in Egypt: Phylogenetic analysis, Pathogenicity and Immunogenicity of an immune-complex vaccine 埃及持续存在毒性极强的传染性法氏囊病病毒(IBDV):系统发育分析、致病性和免疫复合物疫苗的免疫原性
Pub Date : 2024-01-01 DOI: 10.21608/ejah.2024.325133
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引用次数: 0
Lycopene and pine bark extract as nitrite replacers in Egyptian basterma 番茄红素和松树皮提取物作为亚硝酸盐替代物在埃及蝙蝠蛾中的应用
Pub Date : 2024-01-01 DOI: 10.21608/ejah.2024.335662
Y. Youssef, Asmaa Sh. Fayed, Yosra Samy Aleslamboly
. ABSTRACT T he goal of this work was design to study the influence of lycopene and pine bark extracts as natural food additives as alternatives to nitrite, which poses serious health risks to consumers of processed foods containing this substance. These extracts were also found to be effective antimicrobial and antioxidant agents, particularly when used as an example in the current study for manufactured basterma. For this, Egyptian basterma were inoculated with 10 4 CFU g-1 of Staph. aureus and E. coli into raw meat separately, and then treated with a 1% concentration of lycopene and a 1% of pine bark extract. For the duration of the storage period, basterma was kept at room temperature for microbiological assessment and sensory analysis. Staph. aureus and E. coli levels decreased by 4 log and 2 log, respectively, on the fourteenth day. Additionally, Egyptian basterma sensory evaluation yielded a good, acceptable result. Therefore, as compared to the effects of nitrite, lycopene, and pine bark extract, they show strong antibacterial activity during product preservation. The findings provided here may indicate that lycopene and pine bark extracts offer protection against Staph. aureus and E. coli , which means they have the potential to replace nitrite as a natural preservative in the food production.
.摘要 这项工作的目的是研究番茄红素和松树皮提取物作为天然食品添加剂对亚硝酸盐替代品的影响。研究还发现,这些提取物也是有效的抗菌剂和抗氧化剂,特别是在本研究中用作人造黄鼠狼的例子。为此,将 10 4 CFU g-1 的金黄色葡萄球菌和大肠杆菌分别接种到生肉中,然后用浓度为 1%的番茄红素和浓度为 1%的松树皮提取物进行处理。在贮藏期间,将烤肉放在室温下进行微生物评估和感官分析。第 14 天,金黄色葡萄球菌和大肠杆菌含量分别下降了 4 log 和 2 log。此外,埃及烤肉的感官评估结果良好,可以接受。因此,与亚硝酸盐、番茄红素和松树皮提取物的效果相比,它们在产品保存过程中表现出很强的抗菌活性。本文提供的研究结果可能表明,番茄红素和松树皮提取物可防止金黄色葡萄球菌和大肠杆菌的感染,这意味着它们有可能取代亚硝酸盐成为食品生产中的天然防腐剂。
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引用次数: 0
Detection of Cryptosporidium parvum in locally manufactured yoghurt and Kareish cheese 在当地生产的酸奶和卡里什奶酪中检测到副猪隐孢子虫
Pub Date : 2024-01-01 DOI: 10.21608/ejah.2024.332459
B. Nageib, H. M. Kuraa, Parasitology Lab
C ryptosporidium parvum is an important zoonotic parasite causing severe diarrhea in humans and young animals. Fifty milk products (25 Baladi yoghurt and 25 Kareish cheese) were tested for detection of C. parvum oocysts. The prevalence of C. parvum in yoghurt was 44%, 36% and 12% by microscopic examination, experimental infection and PCR, respectively. There is a statistical significant difference of C. parvum in yoghurt between microscopic examination and PCR as well as between experimental infection and PCR. While, its prevalence in Kareish cheese was 24%, 44% and 20% by microscopic examination, experimental infection and PCR, respectively. No statistical significant difference of C. parvum were detected in Kareish cheese between microscopic examination, experimental infection and PCR. Microscopic examination of prepared yoghurt and Kareish cheese smears and experimentally infected rats fecal smears showed the spherical shape of C. parvum oocysts (4-6μm). A banana shape of C. parvum sporozoite (3.2x1.4μm) was found in fecal smear of experimentally infected rat by contaminated yoghurt. Meanwhile, the PCR identified the specific Actin gene of C. parvum in 3 yoghurt and 5 Kareish cheese samples. From the obtained results, it could be concluded that C. parvum oocysts remain viable in locally made milk products.
副猪痢疾杆菌是一种重要的人畜共患寄生虫,可导致人类和幼畜严重腹泻。我们对 50 种奶制品(25 种巴拉迪酸奶和 25 种卡里什奶酪)进行了副猪痢疾杆菌卵囊检测。通过显微镜检查、实验感染和 PCR 检测,酸奶中副猪嗜血杆菌的感染率分别为 44%、36% 和 12%。显微镜检查和聚合酶链式反应以及实验感染和聚合酶链式反应在酸奶中的副猪嗜血杆菌含量有明显的统计学差异。而通过显微镜检查、实验感染和聚合酶链式反应,卡里什奶酪中的流行率分别为 24%、44% 和 20%。显微镜检查、实验感染和聚合酶链式反应在卡里什奶酪中检测到的副干酪酵母菌没有明显的统计学差异。对制备的酸奶和 Kareish 奶酪涂片以及实验感染的大鼠粪便涂片进行显微镜检查后发现,副猪嗜血杆菌卵囊呈球形(4-6μm)。在受酸奶污染的实验感染大鼠粪便涂片中发现了香蕉状的副猪嗜血杆菌孢子囊(3.2x1.4μm)。同时,在 3 份酸奶和 5 份 Kareish 奶酪样本中,PCR 鉴定出了副猪嗜血杆菌的特异性 Actin 基因。根据所获得的结果,可以得出结论:在本地生产的奶制品中,副猪嗜血杆菌卵囊仍然存活。
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引用次数: 0
Phylogenetic and epidemiological characteristics of H9N2 Avian Influenza Viruses from 2020 to 2022 in Egypt 2020 至 2022 年埃及 H9N2 禽流感病毒的系统发育和流行病学特征
Pub Date : 2024-01-01 DOI: 10.21608/ejah.2024.321804
Zienab Mosaad, N. Hagag, Moataz Mohamed, W. Mady, Zeinab A El-Badiea, O. Mahana, Neveen Rabie, M. Samy, Ola abdel aziz, A. Arafa, Abdelhafiz Samir, A. Selim, Samah Eid, M. Shahein, Amany Adel
. ABSTRACT T he H9 low-pathogenic avian influenza (LPAI) viruses cause enormous economic harm despite their low pathogenicity. It became common in Egypt in 2011 and has undergone ongoing genetic evolution since then. To limit the virus's transmission, regular monitoring of its evolution is essential. The current study concentrated on the frequency and molecular characteristics of LPAI H9N2 viruses spreading throughout different Egyptian areas between 2020 and 2022. Using real-time PCR, 503 positive LPAI H9 cases were detected out of 29,319 cases, for a total prevalence rate of 1.7%. However, live bird market (LBM) had the highest LPAI H9N2 prevalence rate (10.6%), followed by household sector and farm (2 % and 1.3% respectively). The 33 samples were isolated in 11-day-old embryonated chicken eggs (ECEs) before being sequenced for partial hemagglutinin (HA). The H9 isolates were phylogenetically related to the Egy-2 G1-B branch (pigeon-like), which has been the prevalent circulating H9N2 genotype in Egypt since 2016. The findings of the sequence analysis revealed a clear genetic evolution compared to the original
.摘要 H9 低致病性禽流感(LPAI)病毒虽然致病性低,但却造成了巨大的经济损失。该病毒于 2011 年在埃及开始流行,此后经历了持续的基因进化。为了限制该病毒的传播,定期监测其进化情况至关重要。目前的研究集中于 2020 年至 2022 年期间在埃及不同地区传播的高致病性禽流感 H9N2 病毒的频率和分子特征。利用实时 PCR 技术,在 29 319 例病例中检测出 503 例高致病性禽流感 H9 病毒阳性病例,总患病率为 1.7%。然而,活禽市场(LBM)的低致病性禽流感 H9N2 感染率最高(10.6%),其次是家庭和农场(分别为 2% 和 1.3%)。在对 33 个样本进行部分血凝素(HA)测序之前,先在 11 日龄的鸡胚蛋中进行了分离。H9分离物与Egy-2 G1-B分支(类鸽)存在系统发育关系,该分支自2016年以来一直是埃及流行的H9N2基因型。序列分析结果表明,与最初的
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引用次数: 0
Impacts of climate change on aquaculture and human health 气候变化对水产养殖和人类健康的影响
Pub Date : 2023-10-01 DOI: 10.21608/ejah.2023.314091
M. Gubbins, I. Bricknell, M. Service
There is little existing evidence of impacts on aquaculture that can be attributed to climate change. Several effects have been observed that could be related to a changing climate such as increased shellfish contamination, harmful plankton events and the establishment of non-native species, but it is not clear that a changing ocean climate is responsible for these effects. Given the current predictions, climate change is unlikely to have a significant effect on Scottish mariculture over the next decade. However, within the next 50 years or more, the forecast changes are likely to result in noticeable effects. Sea-level rise may reduce coastal habitat suitable for bivalve cultivation in the south of the region (where Scottish mariculture is undertaken). Rising average water temperatures will result in faster growth rates for some species (e.g. Atlantic salmon, mussels and oysters) but prolonged periods of warmer summer temperatures may cause thermal stress, particularly for cold water species (e.g. cod and Atlantic halibut) and intertidal shellfish (oysters). However, warmer waters may provide opportunities to culture new species, or species that are currently economically marginal in UK waters.
目前几乎没有证据表明气候变化对水产养殖造成了影响。已经观察到一些可能与气候变化有关的影响,如贝类污染增加、有害浮游生物事件和非本地物种的形成,但尚不清楚海洋气候变化是否应对这些影响负责。根据目前的预测,气候变化不太可能在未来十年对苏格兰的海水养殖产生重大影响。然而,在未来50年或更长时间内,预测的变化可能会产生明显的影响。海平面上升可能会减少该地区南部(苏格兰海水养殖的地方)适合双壳类动物养殖的沿海栖息地。平均水温上升将导致某些物种(如大西洋鲑鱼、贻贝和牡蛎)生长速度加快,但夏季气温长时间变暖可能导致热应激,特别是对冷水物种(如鳕鱼和大西洋大比目鱼)和潮间带贝类(牡蛎)。然而,温暖的水域可能为培育新物种或目前在英国水域处于经济边缘的物种提供机会。
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引用次数: 19
Occurrence of some probiotic bacteria in ewe's and goat's milk in Assuit City with regarding to genes responsible for production of bacteriocins. Assuit市羊奶中某些益生菌的出现与产生细菌素的基因有关。
Pub Date : 2023-10-01 DOI: 10.21608/ejah.2023.312897
Zeinab M. Abd-Elhamed, D. Ali
S mall ruminant's milk is recognized as one kind of nutritious food owed to its originality and medicinal values. 100 samples collected from raw ewe and goat milk (50 of each) reared in Assiut City, Egypt. Milk samples were screened for probiotics Lactobacillus , Bifidobactria and Enterococcus spp. as well as their differentiation especially those harboring bacteriocin genes. On the other hand, milk samples were tested for the existence of total aerobic bacteria and total coliforms. Goat's milk showed higher existence of total aerobic and total coliforms count more than ewe's milk which showed higher existence of Lactobacillus, Bifidobacteria and Enterococcus spp. The study detected Enterococcus faecium as more in ewe's milk (40%) than goat's milk (25%) and tested for harboring bacteriocin genes (mes Y &Plantaricin E/F). Our study concluded that goat's milk had more total pathogenic parameters than ewe's milk, but ewe's milk had more probiotic lactic acid bacteria (LAB); Lactobacillus, Bifidobactria and Enterococcus spp. , also, contained Enterococcus faecium strains harboring bacterioc-in genes mesY& Plantaricin E/F genes more than those of goat's milk. Both of them have its value and uses in dairy industry. Further studies are needed and caring produce of these types of milk and their products in Egypt and could be assessed in several models in a similar manner to what is studied with bovine milk.
小反刍动物奶因其独创性和药用价值而被公认为一种营养食品。从埃及阿西尤特市饲养的生母羊和山羊奶中采集100份样本(各50份)。对牛奶样品中益生菌乳酸菌、双歧杆菌和肠球菌进行了筛选,并对其进行了分化,特别是对含有细菌素基因的益生菌进行了筛选。另一方面,对牛奶样品进行了总需氧细菌和总大肠菌群的检测。羊奶中总需氧菌和总大肠菌群的数量高于母奶,而乳酸菌、双歧杆菌和肠球菌的数量则高于母奶。研究发现,母奶中屎肠球菌的含量(40%)高于羊奶(25%),并检测了含有细菌素基因(mes Y和plantaricin E/F)。研究结果表明,羊奶的总致病参数高于母奶,但母奶的益生菌乳酸菌(LAB)含量高于羊奶;乳酸菌、双歧杆菌和肠球菌也比羊奶含有更多的含有细菌-in基因mesy和Plantaricin E/F基因的屎肠球菌菌株。两者在乳制品工业中都有其价值和用途。需要进一步的研究,并在埃及对这些类型的牛奶及其产品进行护理生产,并且可以在几个模型中以类似于对牛奶进行研究的方式进行评估。
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引用次数: 0
Microbiological evaluation of small and large scale produced ice cream in Assiut city 阿苏特市小型和大型生产冰淇淋的微生物评价
Pub Date : 2023-10-01 DOI: 10.21608/ejah.2023.311940
O. Sadek, A. M. Koriem
T his study aimed to compare between the microbiological criteria of small and large scale produced ice cream consumed in Assiut city, Egypt. A total of 70 small and large scale produced ice cream samples (35 each) were collected from available retailer ice cream shops and supermarkets in Assiut city and evaluated microbiologically. The incidences of aerobic bacteria count (APC), psychrotrophes, Enterococci , coliform, faecal coliform counts, Escherichia coli , yeasts and moulds counts were 100, 100, 97.14, 65.71, 31.43, 22.86, 100 and 65.71% in small scale produced ice cream samples respectively; while, in large scale produce ice cream samples, the incidences were 100, 74.29, 42.86, 45.71, 31.43, 11.43, 54.29 and 100%, respectively. Staphylococcus aureus was negative in both type of ice cream samples while coagulase negative Staphylococci (CNS) and anaer-obes were detected in small scale produce ice cream samples in incidences of 11.43 and 80% respectively, the incidences were 8.57 and 57.14% for large scale produced ones, respectively. Two isolates of Escherichia coli O157:H7 and Stx 1 and Stx 2 were detected in some Escherichia coli strains isolated from small scale produced ice cream samples. Comparison of the microbiological criteria of the two types of ice cream samples with Egyptian Standards was also done. Sources of these microorganisms, their economic and public health importance as well as the
本研究旨在比较埃及阿西尤特市消费的小型和大规模生产冰淇淋的微生物标准。从阿西尤特市现有的零售商冰淇淋店和超市中收集了70个小型和大型生产的冰淇淋样品(各35个),并进行了微生物学评估。小规模生产冰淇淋样品中需氧菌数(APC)、嗜冷菌数、肠球菌数、大肠菌群数、粪大肠菌群数、大肠杆菌数、酵母数和霉菌数的发生率分别为100、100、97.14、65.71、31.43、22.86、100和65.71%;而在大规模生产的冰淇淋样品中,发病率分别为100、74.29、42.86、45.71、31.43、11.43、54.29和100%。两种冰淇淋样品中均检出金黄色葡萄球菌阴性,小规模生产冰淇淋样品中检出凝固酶阴性葡萄球菌(CNS)和耐水球菌,发生率分别为11.43%和80%,大规模生产冰淇淋样品中检出凝固酶阴性葡萄球菌和耐水球菌的发生率分别为8.57%和57.14%。从小规模生产的冰淇淋样品中分离的大肠埃希菌中检出两株分离株:H7和stx1和stx2。并将两种冰淇淋样品的微生物学指标与埃及标准进行了比较。这些微生物的来源、它们在经济和公共卫生方面的重要性以及它们对人类健康的影响
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引用次数: 0
期刊
Egyptian Journal of Animal Health
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