Pub Date : 2022-10-31DOI: 10.22302/iribb.jur.mp.v90i2.505
R. T. Saptari, I. Riyadi, Masna Maya Sinta, M. E. Prasetyo, Sylvia Lindawati, S. Sumaryono
AbstrakBioreaktor perendaman sesaat (BPS) telah digunakan secara luas untuk propagasi skala massal berbagai tanaman penting, termasuk tanaman tebu. BPS menyediakan sistem kultur semi-otomatis dan kondisi optimal bagi pertumbuhan tanaman. Beberapa faktor menentukan pertumbuhan tanaman pada BPS, salah satunya densitas dari eksplan. Oleh karena itu, penelitian dilakukan untuk menentukan bobot awal yang optimal untuk kalus tebu yang dikulturkan pada BPS, serta mengevaluasi pengaruh perbedaan bobot awal kalus tersebut terhadap proliferasi dan regenerasi kalus tebu. Kalus tebu diinduksi dari daun muda yang masih menggulung dari empat varietas tebu unggul Indonesia. Bobot awal kalus yang dikultur ke dalam bejana TIB yaitu 0,05 g; 0,1 g; 0,2 g; 0,5 g; dan 1,0 g untuk setiap bejana. Kalus kemudian melalui tahap proliferasi pada BPS sebanyak tiga siklus, kemudian kalus diregenerasi pada BPS dengan perlakuan auksin dan sitokinin. Hasil penelitian menunjukkan bahwa 0,2 g merupakan bobot awal kalus yang efisien untuk proliferasi kalus tebu pada TIB, dimana eksponensial multiplikasi kalus tercapai pada bobot awal tersebut, yaitu untuk masing-masing varietas 130,3 kali (PSKA 942), 136,8 kali (PS 094), 21,3 (PS 881), dan 12,9 kali (PS 091) setelah 12 minggu. Densitas kalus pada TIB berkorelasi negatif dengan karakteristik fisikokimia medium. Hal ini menggambarkan variasi intensitas pertumbuhan dan metabolisme kalus dengan adanya perbedaan densitas pada BPS. Penggunaan BAP 0,2 mg L-1 bersama kinetin 0,2 mg L-1 paling sesuai untuk memacu regenerasi kalus tebu dengan menghasilkan jumlah tunas terbanyak dalam waktu relatif lebih cepat (1 – 2 minggu lebih cepat) dibandingkan perlakuan lainnya dan dengan tingkat kejadian pencoklatan yang rendah.[Kata kunci: kultur in vitro, kultur cair, proliferasi]AbstractTemporary immersion bioreactor (TIB) has been utilized for the mass-scale propagation of many important plants, including sugarcane. TIB facilitates a semiautomated culture system and provides optimal conditions for plant growth. Several factors determine plant growth in the TIB, such as explant density. Therefore, an experiment was carried out to determine the optimal initial weight of sugarcane calli and to evaluate its effect on the proliferation and regeneration in TIB. Sugarcane calli were induced from spindle leaves isolated from four Indonesian prime sugarcane varieties. The initial weights of the calli cultured in the TIB flasks were 0.05 g, 0.1 g, 0.2 g, 0.5 g and 1.0 g per flask. The calli were proliferated through three cycles in TIB, and subsequently regenerated in TIB with auxin and cytokinin treatments. The results of the experiments showed that 0.2 g was the most efficient initial weight for sugarcane callus proliferation in the TIB, resulting in an exponential multiplication rate of 130.3-fold (PSKA 942), 136.8-fold (PS 094), 21.3-fold (PS 881), and 12.9-fold (PS 091) within 12 weeks. In the TIB, callus density showed a negative correlation with the
{"title":"Determination of the optimum initial callus weight for the efficient propagation of sugarcane in temporary immersion bioreactor","authors":"R. T. Saptari, I. Riyadi, Masna Maya Sinta, M. E. Prasetyo, Sylvia Lindawati, S. Sumaryono","doi":"10.22302/iribb.jur.mp.v90i2.505","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v90i2.505","url":null,"abstract":"AbstrakBioreaktor perendaman sesaat (BPS) telah digunakan secara luas untuk propagasi skala massal berbagai tanaman penting, termasuk tanaman tebu. BPS menyediakan sistem kultur semi-otomatis dan kondisi optimal bagi pertumbuhan tanaman. Beberapa faktor menentukan pertumbuhan tanaman pada BPS, salah satunya densitas dari eksplan. Oleh karena itu, penelitian dilakukan untuk menentukan bobot awal yang optimal untuk kalus tebu yang dikulturkan pada BPS, serta mengevaluasi pengaruh perbedaan bobot awal kalus tersebut terhadap proliferasi dan regenerasi kalus tebu. Kalus tebu diinduksi dari daun muda yang masih menggulung dari empat varietas tebu unggul Indonesia. Bobot awal kalus yang dikultur ke dalam bejana TIB yaitu 0,05 g; 0,1 g; 0,2 g; 0,5 g; dan 1,0 g untuk setiap bejana. Kalus kemudian melalui tahap proliferasi pada BPS sebanyak tiga siklus, kemudian kalus diregenerasi pada BPS dengan perlakuan auksin dan sitokinin. Hasil penelitian menunjukkan bahwa 0,2 g merupakan bobot awal kalus yang efisien untuk proliferasi kalus tebu pada TIB, dimana eksponensial multiplikasi kalus tercapai pada bobot awal tersebut, yaitu untuk masing-masing varietas 130,3 kali (PSKA 942), 136,8 kali (PS 094), 21,3 (PS 881), dan 12,9 kali (PS 091) setelah 12 minggu. Densitas kalus pada TIB berkorelasi negatif dengan karakteristik fisikokimia medium. Hal ini menggambarkan variasi intensitas pertumbuhan dan metabolisme kalus dengan adanya perbedaan densitas pada BPS. Penggunaan BAP 0,2 mg L-1 bersama kinetin 0,2 mg L-1 paling sesuai untuk memacu regenerasi kalus tebu dengan menghasilkan jumlah tunas terbanyak dalam waktu relatif lebih cepat (1 – 2 minggu lebih cepat) dibandingkan perlakuan lainnya dan dengan tingkat kejadian pencoklatan yang rendah.[Kata kunci: kultur in vitro, kultur cair, proliferasi]AbstractTemporary immersion bioreactor (TIB) has been utilized for the mass-scale propagation of many important plants, including sugarcane. TIB facilitates a semiautomated culture system and provides optimal conditions for plant growth. Several factors determine plant growth in the TIB, such as explant density. Therefore, an experiment was carried out to determine the optimal initial weight of sugarcane calli and to evaluate its effect on the proliferation and regeneration in TIB. Sugarcane calli were induced from spindle leaves isolated from four Indonesian prime sugarcane varieties. The initial weights of the calli cultured in the TIB flasks were 0.05 g, 0.1 g, 0.2 g, 0.5 g and 1.0 g per flask. The calli were proliferated through three cycles in TIB, and subsequently regenerated in TIB with auxin and cytokinin treatments. The results of the experiments showed that 0.2 g was the most efficient initial weight for sugarcane callus proliferation in the TIB, resulting in an exponential multiplication rate of 130.3-fold (PSKA 942), 136.8-fold (PS 094), 21.3-fold (PS 881), and 12.9-fold (PS 091) within 12 weeks. In the TIB, callus density showed a negative correlation with the ","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73856425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.22302/iribb.jur.mp.v90i2.484
S. Wahyuni, Ciptadi Ahmad Yusup, Agustin Sri Mulyatni, Deden Dewantara Eris, Priyono Priyono, S. Siswanto
Chitosan is a derivative compound from chitin that has potential as a biopesticide and biostimulant. This research aimed to analyze the effect of chitosan’s soluble liquid (SL) on the disease suppression and yield of rice. This research was conducted in two locations: the first was at Nglawak Village, Nganjuk District, East Java, and the second was at Harjasari Village, Tegal District, Central Java, in dry season of 2020. Chitosan SL application was carried out by foliar spray on rice plants at 2, 6, and 9 weeks after planting with the treatment of Chitosan SL 25 ml L-1 (P1), Chitosan SL 25 ml L-1+ synthetic pesticides (P2), synthetic pesticides application as control (P0). Each treatment was replicated nine times in each location. The vegetative parameters observed consist of leaf color, number of tillers, and plant height, while the number of productive tillers was observed during the harvesting period. The observed disease incidence was bacterial leaf blight, sheath blight, and blast. The result showed that the application of Chitosan SL significantly affected disease suppression (sheath blight) and yield compared to control, and there was no significant effect between P1 and P2. The application of Chitosan SL could suppress sheath blight disease by up to 45% and increase crop yields by up to 25%. Abstrak Kitosan merupakan senyawa turunan dari kitin yang memiliki potensi sebagai biopestisida dan juga biostimulan. Penelitian ini bertujuan untuk menguji pengaruh aplikasi kitosan soluble liquid (SL) terhadap kejadian penyakit dan hasil panen padi. Pengujian dilakukan di dua lokasi, yakni di Desa Nglawak, Kabupaten Nganjuk Jawa Timur dan di Desa Harjasari, Kabupaten Tegal, Jawa Tengah pada musim kemarau tahun 2020. Aplikasi kitosan SL dilakukan dengan penyemprotan daun pada 2, 6, dan 9 minggu setelah tanam, dengan perlakuan meliputi: kitosan SL 25 mL L-1(P1), kitosan SL 25 ml L-1+ pestisida kimia (P2), dan pestisida sintetik saja sebagai kontrol (P0). Setiap perlakuan diulang sebanyak sembilan kali pada masing-masing lokasi. Parameter vegetatif yang diamati meliputi warna daun, jumlah anakan dan tinggi tanaman, sedangkan jumlah anakan produktif diamati pada saat panen. Kejadian penyakit yang diamati adalah hawar daun bakteri, hawar daun pelepah, dan blas. Hasil pengamatan menunjukkan bahwa aplikasi kitosan SL pada tanaman padi berpengaruh nyata terhadap penekanan kejadian penyakit hawar pelepahdan hasil panen dibandingkan kontrol, serta tidak ada perbedaan pengaruh yang nyata antara P1 dengan P2. Aplikasi kitosan SL mampu menekan kejadian penyakit hawar pelepah hingga 45% dan mampu meningkatkan hasil panen hingga 25%.
壳聚糖是甲壳素的衍生物，具有作为生物农药和生物刺激素的潜力。本研究旨在分析壳聚糖可溶性液(SL)对水稻抗病性和产量的影响。这项研究在两个地点进行:第一次是在东爪哇的Nganjuk区Nglawak村，第二次是在中爪哇的Tegal区Harjasari村，时间是2020年旱季。以壳聚糖25 ml L-1 (P1)、壳聚糖25 ml L-1+合成农药(P2)、合成农药为对照(P0)为对照，于种植后2周、6周和9周叶片喷施壳聚糖SL。每个治疗在每个位置重复9次。观察到的营养参数包括叶片颜色、分蘖数和株高，而在收获期间观察到有效分蘖数。观察到的病害为细菌性叶枯病、鞘枯病和瘟病。结果表明，与对照相比，壳聚糖SL对水稻纹枯病的抑制作用和产量有显著影响，P1和P2之间无显著影响。应用壳聚糖SL可抑制叶枯病达45%，增产达25%。【摘要】Kitosan merupakan senyawa turunan dari kitin yang memoriliki potential sebagai bioestisida dan juga biostimulan。Penelitian ini bertujuan untuk menguji pengaruh应用kitosan可溶性液体(SL) terhadap kejadian penyakit和hasil panen padi。企鹅dilakukan di dua lokasi, yakni di Desa Nglawak, Kabupaten Nganjuk，爪哇Timur dan di Desa Harjasari, Kabupaten Tegal，爪哇Tengah pada musim kemarau tahun 2020。应用plikasi kitosan SL dilakukan dentan penyemprotan dpaada 2,6, dan 9, minggu setelah tanam, dengan perlakkan melputi: kitosan SL 25ml L-1(P1)， kitosan SL 25ml L-1+鼠疫(P2)， dan sinintetik saja sebagai对照(P0)。Setiap perlakuan diulang sebanyak sembilan kali pada masing-masing lokasi。参数为:阳春麻花、芦笋、芦笋、芦笋、芦笋、芦笋、芦笋、芦笋、芦笋。基雅底人，亚大拉，哈瓦，巴特尔利，哈瓦，比利巴，但布拉斯。Hasil pengamatan menunjukkan bahwa applikasi kitosan SL pada tanaman padi berpengaruh nyata terhadap penekanan kejadian penyakit hawar pelepahdan Hasil panen dibandingkan control, serta tidak ada perbedaan and pengaruh yang nyata antara P1 dengan P2。applikasi kitosan SL mampu menekan kejadian penyakit hawar pelepah hingga 45% dan mampu meningkatkan hasil panen hingga 25%
{"title":"Aplikasi kitosan untuk penekanan kejadian penyakit dan peningkatan hasil panen tanaman padi (Oryza sativa L.)","authors":"S. Wahyuni, Ciptadi Ahmad Yusup, Agustin Sri Mulyatni, Deden Dewantara Eris, Priyono Priyono, S. Siswanto","doi":"10.22302/iribb.jur.mp.v90i2.484","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v90i2.484","url":null,"abstract":"Chitosan is a derivative compound from chitin that has potential as a biopesticide and biostimulant. This research aimed to analyze the effect of chitosan’s soluble liquid (SL) on the disease suppression and yield of rice. This research was conducted in two locations: the first was at Nglawak Village, Nganjuk District, East Java, and the second was at Harjasari Village, Tegal District, Central Java, in dry season of 2020. Chitosan SL application was carried out by foliar spray on rice plants at 2, 6, and 9 weeks after planting with the treatment of Chitosan SL 25 ml L-1 (P1), Chitosan SL 25 ml L-1+ synthetic pesticides (P2), synthetic pesticides application as control (P0). Each treatment was replicated nine times in each location. The vegetative parameters observed consist of leaf color, number of tillers, and plant height, while the number of productive tillers was observed during the harvesting period. The observed disease incidence was bacterial leaf blight, sheath blight, and blast. The result showed that the application of Chitosan SL significantly affected disease suppression (sheath blight) and yield compared to control, and there was no significant effect between P1 and P2. The application of Chitosan SL could suppress sheath blight disease by up to 45% and increase crop yields by up to 25%. Abstrak Kitosan merupakan senyawa turunan dari kitin yang memiliki potensi sebagai biopestisida dan juga biostimulan. Penelitian ini bertujuan untuk menguji pengaruh aplikasi kitosan soluble liquid (SL) terhadap kejadian penyakit dan hasil panen padi. Pengujian dilakukan di dua lokasi, yakni di Desa Nglawak, Kabupaten Nganjuk Jawa Timur dan di Desa Harjasari, Kabupaten Tegal, Jawa Tengah pada musim kemarau tahun 2020. Aplikasi kitosan SL dilakukan dengan penyemprotan daun pada 2, 6, dan 9 minggu setelah tanam, dengan perlakuan meliputi: kitosan SL 25 mL L-1(P1), kitosan SL 25 ml L-1+ pestisida kimia (P2), dan pestisida sintetik saja sebagai kontrol (P0). Setiap perlakuan diulang sebanyak sembilan kali pada masing-masing lokasi. Parameter vegetatif yang diamati meliputi warna daun, jumlah anakan dan tinggi tanaman, sedangkan jumlah anakan produktif diamati pada saat panen. Kejadian penyakit yang diamati adalah hawar daun bakteri, hawar daun pelepah, dan blas. Hasil pengamatan menunjukkan bahwa aplikasi kitosan SL pada tanaman padi berpengaruh nyata terhadap penekanan kejadian penyakit hawar pelepahdan hasil panen dibandingkan kontrol, serta tidak ada perbedaan pengaruh yang nyata antara P1 dengan P2. Aplikasi kitosan SL mampu menekan kejadian penyakit hawar pelepah hingga 45% dan mampu meningkatkan hasil panen hingga 25%. ","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84810273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.22302/iribb.jur.mp.v90i2.501
Yora Faramitha, Firda Dimawarnita, Haryo Tejo Prakoso, S. Siswanto
The application of silver nanoparticles as antifungal in the plantation sector is very potential to be developed. Silver nanoparticles conjugated with cysteine have the advantage of having low toxicity, making them safer for the environment. Until now, basal stem rot disease caused by apathogenic fungus, Ganoderma boninense, has become a serious problem and causes economic losses in oil palm plantations. Studies related to the effect of silver nanoparticles on the control of G. boninense have not been widely studied. Therefore, the objectives of this research are to evaluate the stability of silver nanoparticles conjugated with L-cysteine (Cys-AgNPs) and to examine Cys-AgNPs ability in inhibiting the mycelial growth of G. boninense. Cysteine-silver nanoparticles were characterized using UV-Vis, particle size analyzer (PSA), and Fourier Transform Infrared (FTIR). The assay of Cys-AgNPs activity as an antifungal against G. boninense was carried out in vitro. As a result, cysteine-silver nanoparticles were successfully synthesized by producing a brownish-yellow color and maximum localized surface plasmon resonance (LSPR) absorbance in the range of 402 nm. There was an effect of L-cysteine concentration on the stability of Cys-AgNPs. The use of 0.01 M L-cysteine concentration resulted in Cys-AgNPs that were more stable and smaller in particle size than 0.001 M L-cysteine. Silver-cysteine nanoparticles could inhibit the growth of G. boninense mycelia, with a highest percentage of mycelia inhibition observed from the application of Cys-AgNPs at 8 mg L-1 on day 6 (65,17%). AbstrakAplikasi nanopartikel perak sebagai antifungi di bidang perkebunan sangat potensial untuk dikembangkan. Nanopartikel perak yang dikonjugasi dengan L-cysteine memiliki keunggulan yaitu sifat toksisitas yang rendah sehingga lebih aman untuk lingkungan. Hingga saat ini, penyakit busuk pangkal batang pada perkebunan kelapa sawit yang disebabkan oleh cendawan patogen Ganoderma boninense masih menjadi permasalahan yang serius dan menyebabkan kerugian secara ekonomi. Studi terkait pengaruh nanopartikel perak terhadap pengendalian G. boninense masih belum banyak dikaji. Oleh karena itu, penelitian ini bertujuan untuk mengevaluasi stabilitas nanopartikel perak yang dikonjugasikan dengan L-cysteine (Cys-AgNPs) dan menguji kemampuannya dalam menghambat pertumbuhan miselia G. boninense. Nanopartikel perak-cysteine dikarakterisasi menggunakan UV-Vis, particle size analyzer (PSA), dan Fourier Transform Infrared (FTIR). Uji aktivitas Cys-AgNPs sebagai antifungi terhadap G. boninense dilakukan secara in vitro. Sebagai hasil, nanopartikel perak-cysteine berhasil disintesis dengan menghasilkan warna kuning kecoklatan dan absorbansi localized surface plasmon resonance (LSPR) maksimum pada kisaran 402 nm. Terdapat pengaruh konsentrasi L-cysteine terhadap stabilitas Cys-AgNPs. Penggunaan konsentrasi L-cysteine 0,01 M menghasilkan Cys-AgNPs yang lebih stabil dan ukuran partikel yang lebih kecil di
纳米银作为抗真菌剂在人工林领域的应用具有很大的发展潜力。与半胱氨酸结合的银纳米颗粒具有低毒性的优点，使其对环境更安全。目前，由病原菌灵芝(Ganoderma boninense)引起的基茎腐病已成为油棕种植业的一个严重问题，造成经济损失。有关银纳米颗粒对牛小叶蝉防治效果的研究尚未得到广泛的研究。因此，本研究的目的是评估银纳米粒子结合l -半胱氨酸(Cys-AgNPs)的稳定性，并研究Cys-AgNPs抑制牛链球菌菌丝生长的能力。采用紫外可见光谱(UV-Vis)、粒径分析仪(PSA)和傅里叶变换红外(FTIR)对半胱氨酸银纳米粒子进行了表征。体外测定了Cys-AgNPs的抗真菌活性。结果表明，半胱氨酸-银纳米粒子具有棕黄色，最大局部表面等离子体共振(LSPR)吸光度在402 nm范围内。l -半胱氨酸浓度对Cys-AgNPs的稳定性有影响。使用0.01 M l -半胱氨酸浓度制备的Cys-AgNPs比0.001 M l -半胱氨酸更稳定，粒径更小。银-半胱氨酸纳米颗粒可以抑制G. boninense菌丝的生长，在第6天使用Cys-AgNPs (8 mg L-1)对菌丝的抑制率最高(65,17%)。摘要/ abstract摘要:柞蚕纳米颗粒抗真菌活性研究进展。纳米颗粒perak yang dikonjuas, dengan, l -半胱氨酸记忆体，keggulan, yitu, sifat, toksisas, yang rendah, sehinga, lebih, an untuk lingkungan。【中文翻译】:兴伽山尼，penyakit busuk pangkal batang pada perkebunan kelapa sawwit yang disebabkan oleh cendawan病原体灵芝(Ganoderma boninense masih menjadi permasalahan yang serius dan menyebabkan kerugian secara ekonomian)。研究了一种新型的纳米颗粒结构。纳米颗粒perakak yang dikonjugasikan dengan l -半胱氨酸(Cys-AgNPs) [m]， kemampuannya dalam menghambat pertumbuhan miselia G. boninense。纳米粒子perak-半胱氨酸dikarakterisasi menggunakan UV-Vis，粒度分析仪(PSA)，傅立叶变换红外(FTIR)。Uji活性物Cys-AgNPs sebagai体外抗真菌terhadap boninense dilakukan secara。Sebagai hasil，纳米粒子perak-半胱氨酸berhasil的分离，dengan, menghasilkan, warna, kuning, kecoklatan，并吸收局部表面等离子体共振(LSPR)，最大振幅为402 nm。Terdapat pengaruh konsentrasi l -半胱氨酸terhadap stabilitas Cys-AgNPs。彭家南konsentrasi l -半胱氨酸，2001 M.孟家南Cys-AgNPs，杨利比稳定的l -半胱氨酸，200001 M.纳米颗粒perak-半胱氨酸，mampu menghambat pertumbuhan miselia G. boninense dengan表示酶，彭家南miselia tertinggi diamati dari应用Cys-AgNPs最佳sebesa 8 mg L-1 paadhari -6(65,17%)。
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Pub Date : 2022-04-29DOI: 10.22302/iribb.jur.mp.v90i1.493
I. P. Sari, Y. Lestari, H. Hamim, L. Santi
Abstract Global climate change will result in decreased rainfall and increased evaporation. Thus, it is estimated that the frequency and severity of drought stress will get worse. Silica increases plant drought resistance by improving water use efficiency in plants. Despite its abundant availability in soil, most silica sources are not available to plants due to their low solubility. Silica solubilizing bacteria (SSB) have an important role in increasing the available silica. This study aims to observe the silica solubilizing activity of three SSB isolates collections of PPBBI on insoluble silica sources, including magnesium trisilicate, quartz, and feldspar, and see their effects on increasing water use efficiency in corn plants via drought experiments. SSB activity was measured using the modified standard method of 4500-SiO2 D Heteropoly blue. Drought control in the greenhouse follows the Snow and Tingey system. The experimental design used a completely randomized design factorial with irrigation conditions and SSB species as variables. Water use efficiency is measured in real-time with a sap flow meter. The results showed that SSB Pseudomonas fluorescens-B41 had the highest silica dissolving activity 81.93 ppm on Mg-trisilicate. The application of SSB can reduce maize transpiration rate and increase water use efficiency up to 84% under moderate drought stress and 46% under normal irrigation, but in severe drought stress, where the nutrient solution was maintained at 25 cm from plant root, water use efficiency was not significant. This is suspected due to the extreme drought conditions in the potting soil so that the applied SSB cannot maintain its activities.[Keywords: aquaporin, drought stress, Snow and Tingey system, SSB, quartz]AbstrakPerubahan iklim global mengakibatkan penurunan curah hujan dan peningkatan evaporasi, sehingga diperkirakan frekuensi dan tingkat keparahan cekaman kekeringan akan semakin tinggi. Silika (Si) diketahui dapat meningkatkan ketahanan tanaman terhadap kekeringan dengan cara memperbaiki efisiensi penggunaan air pada tanaman. Meskipun ketersediaanya berlimpah di tanah, sebagian besar Si dalam bentuk yang tidak tersedia bagi tanaman, karena sifat kelarutannya yang rendah. Untuk meningkatkan silika tersedia bagi tanaman, bakteri pelarut silika (BPS) memiliki peranan yang penting. Penelitian ini bertujuan menguji aktivitas pelarutan silika dari tiga koleksi isolat BPS Pusat Penelitian Bioteknologi dan Bioindustri Indonesia (PPBBI) pada sumber silika tidak larut berupa magnesium trisilikat, kuarsa, dan feldspar, serta melihat pengaruh aplikasi BPS terhadap efisiensi penggunaan air pada tanaman jagung yang diberi perlakuan cekaman kekeringan. Aktivitas pelarutan silika diukur menggunakan modifikasi metode standar 4500-SiO2 D Heteropoly blue. Pengendalian kekeringan di rumah kaca mengadaptasi sistem Snow dan Tingey. Rancangan percobaan menggunakan rancangan acak lengkap faktorial dengan kondisi kekeringan dan jenis bakter
全球气候变化将导致降雨量减少和蒸发量增加。因此，估计干旱压力的频率和严重程度将会恶化。二氧化硅通过提高植物水分利用效率来提高植物抗旱性。尽管二氧化硅在土壤中有丰富的可用性，但由于其溶解度低，大多数二氧化硅源不能为植物所利用。二氧化硅增溶菌(SSB)对提高有效二氧化硅具有重要作用。本研究旨在通过干旱试验，观察PPBBI三种SSB菌株对三硅酸镁、石英和长石等不溶性硅源的增溶活性，并观察其对提高玉米植株水分利用效率的影响。采用改进的4500-SiO2 D杂多蓝标准法测定SSB活性。温室的干旱控制遵循Snow和Tingey系统。试验设计采用完全随机设计因子，以灌溉条件和SSB品种为变量。水的利用效率是实时测量与液流计。结果表明，SSB荧光假单胞菌- b41对三硅酸镁的溶解活性最高，为81.93 ppm。在中度干旱胁迫下，施SSB可使玉米蒸腾速率降低84%，水分利用效率提高46%，但在重度干旱胁迫下，当营养液保持在离根25 cm处时，水分利用效率不显著。这被怀疑是由于盆栽土壤的极端干旱条件，使施用的SSB不能保持其活性。[关键词]水通道蛋白，干旱胁迫，雪和廷吉系统，SSB，石英]摘要:perubahan iklim global mengakibatkan penurunan curah hujan dan peningkatan蒸发，sehinga diperkirakan frekuensi dan tingkat keparahan cekaman kekeringan akan semakin tinggi。Silika (Si) diketahui dapat meningkatkan ketahanan tanaman terhadap kekeringan dengan cara member perbaiki efisiensi penggunaan air paada tanaman。Meskipun ketersediaanya berlimpah di tanah, sebagian besar Si dalam bentuk yang tidak tersedia bagi tanaman, karena sifat kelarutannya yang rendah。Untuk meningkatkan silka tersedia bagi tanaman, bakteri pelarut silka (BPS) memoriliki peranan yang penting。Penelitian ini bertujuan menguji aktivitas pelarutan silika达里语tiga koleksi isolat BPS Pusat Penelitian Bioteknologi丹Bioindustri印尼(PPBBI)篇sumber silika有些larut berupa镁trisilikat, kuarsa,丹长石,舒达melihat pengaruh aplikasi BPS terhadap efisiensi penggunaan空气篇tanaman jagung杨diberi perlakuan cekaman kekeringan。白绢绢diukur menggunakan改性卡西法标准4500-SiO2 D杂多蓝。彭根大连，柯克凌，迪鲁玛，卡卡，蒙纳塔斯系统，雪丹，廷格。ranchangan percobaan menggunakan ranchangan acak lengkap工厂dengan kondisi kekeringan dan jenis bakteri BPS sebagai peubah bebas。Efisiensi penggunaan air diukur secara实时登干液流量仪。荧光假单胞菌b。41 memoriliki aktivitas pelarutan silika tertinggi pada底物Mg-trisilika yaitu 81,93 ppm。applikasi BPS menurunkan laju transpirasi jagung dan meningkatkan efisiensi penggunaan air hinga 84% pada cekaman kekeringan sedang dan 46% pada irigasi normal, namun pada cekaman kekeringan parah, dimana larutan hara dipertahankan pada jarak 25 cm dari system perakaran efisiensi penggunaan air tidak signifikan。Diduga hal ini disebabkan kondisi kekeringan和pada media tanam teralalstrim seinga BPS yang Diduga hal tankikika datakan活动在中国。[Kata kunci:水通道蛋白，cekaman kekeringan, system Snow dan Tingey, BPS, kuarsa]
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Pub Date : 2022-04-29DOI: 10.22302/iribb.jur.mp.v90i1.492
Muhammad Eko Prasetyo, Imron Riyadi, Sumaryono Sumaryono
The performance of kopyor Dwarf coconuts from embryo culture in the field has not been reported. This study was conducted to determine the difference in vegetative and generative performances of three fruit color phenotypes (brown, green, and yellow) of kopyor Dwarf coconuts in the first year after the first flower appeared. The coconuts were planted in Bogor, West Java at 260 masl with average temperature 26.9 ºC and average rainfall 4,300 mm/year. Each color phenotype was represented by 20 kopyor coconut trees from the same planting date that had not been flowering yet. When the first flowers appeared, Kopyor Brown Dwarf (KBD) had 14.6 fronds, while Kopyor Yellow Dwarf (KYD) and Kopyor Green Dwarf (KGD) had 15.5 and 17.1 fronds respectively. Other vegetative components when the first flower appeared were not significantly difference among the three color phenotypes such as stem girth (90.7-99.2 cm), stem height (18.6-23.9 cm), frond length (3.9-4.2 m) and canopy diameter (5.8-6.1 m). KBD coconuts started flowering faster than that of KGD and KYD. The numbers of flowers formed in the first year of flowering were 15 to 17 flowers per tree. There was an initial sharp decrease in the survival of young fruits, but this levelled off after 2-3 months. On average 18-22 fruits per bunch at flowering decreased to 5-6 fruits per bunch at maturity. The survival of fruits in KGD (6.2 fruits per bunch) was higher than KBD (4.0 fruits) and KYD (4.5 fruits) at 11 months after flowering. The average fruit size and weight of brown phenotype were higher than green and yellow phenotypes. The mature brown fruits had a lower husk percentage, but higher shell and fruit meat percentages than those of the green and yellow fruits.[Keywords: fruit color phenotype, kopyor Dwarf coconut, fruit abortion, fruit quality] AbstrakKeragaan kelapa Genjah kopyor asal kultur embrio di lapang belum pernah dilaporkan. Penelitian ini dilakukan untuk mengamati perbedaan keragaan vegetatif dan generatif tiga varietas kelapa Genjah kopyor yang berbeda dari warna buah (coklat, hijau dan kuning) pada tahun pertama sejak bunga pertama muncul. Kelapa tersebut ditanam di Bogor, Jawa Barat pada 260 mdpl dengan rerata suhu 26,9 ºC dan curah hujan 4.300 mm/tahun. Tiap fenotipe warna diwakili oleh 20 pohon kelapa kopyor berumur sama yang belum berbunga. Pada saat bunga pertama muncul, kelapa Genjah Coklat Kopyor (GCK) memiliki 14,6 pelepah daun, sedangkan Genjah Kuning Kopyor (GKK) dan Genjah Hijau Kopyor (GHK) berturut-turut memiliki 15,5 dan 17,1 pelepah. Parameter vegetatif lain ketika bunga pertama muncul tidak berbeda nyata antar fenotipe warna misalnya lingkar batang (90,7-99,2 cm), tinggi batang (18,6-23,9 cm), panjang pelepah (3,9-4,2 m) dan diameter tajuk (5,8-6,1 m). GCK mulai berbunga lebih cepat dibandingkan dengan GHK dan GKK. Jumlah bunga yang terbentuk selama satu tahun pertama pembungaan adalah 15 sampai 17 bunga per pohon. Jumlah buah muda yang bertahan hidup menurun tajam pada
kopyor矮椰子的田间胚培养性能尚未见报道。本研究旨在测定科佩尔矮椰子三种果色表型(棕色、绿色和黄色)在第一朵花出现后第一年营养和生殖性能的差异。这些椰子种植在西爪哇的茂物，年平均气温为26.9摄氏度，平均降雨量为4300毫米，年平均气温为260摄氏度。每种颜色表型由同一种植日期尚未开花的20棵kopyor椰子树代表。初花时，Kopyor Brown Dwarf (KBD)有14.6个叶片，Kopyor Yellow Dwarf (KYD)和Kopyor Green Dwarf (KGD)分别有15.5和17.1个叶片。茎长(90.7 ~ 99.2 cm)、茎高(18.6 ~ 23.9 cm)、叶长(3.9 ~ 4.2 m)和冠层直径(5.8 ~ 6.1 m)等3种颜色表型间第一次开花时的其他营养成分差异不显著，KBD比KGD和KYD的开花速度快。在开花的第一年，每棵树形成的花数为15 ~ 17朵。幼果的成活率最初急剧下降，但在2-3个月后趋于平稳。开花时平均每串果18-22个，成熟时每串果5-6个。开花后11个月，KGD组(每串6.2个)的果实成活率高于KBD组(4.0个)和KYD组(4.5个)。棕色表型的平均果实大小和重量高于绿色和黄色表型。成熟的棕色果实比成熟的绿色果实和成熟的黄色果实具有更低的果皮率，而更高的果壳率和果肉率。【关键词】果色表型，kopyor矮椰子，果实败育，果实品质】摘要:keragaan kelapa Genjah kopyor asal培养胚Penelitian ini dilakukan untuk mengamati perbedaan keragaan植被dan generatitiga变种kelapa Genjah kopyor yang berbeda dari warna buah (coklat, hijau dan kuning) pada tahun pertama sejak bunga pertama muncul。Kelapa tersebut ditanam di Bogor，爪哇Barat pada 260 mdpl dengan rerata suhu 26,9ºC dan curah hujan 4.300 mm/tahun。Tiap fenotipe warna diwakili oleh 20 pohon kelapa kopyor berumur sama yang belum berbunga。Pada saat bunga pertama muncul, kelapa Genjah Coklat Kopyor (GCK) memoriliki 14,6 pelepah daun, sedangkan Genjah Kuning Kopyor (GKK) dan Genjah Hijau Kopyor (GHK) berturut-turut memoriliki 15,5 dan 17,1 pelepah。参数植被:ketika bunga pertama muncul、berbeda nyata antar、warna misalnya lingkar batang (90,7-99,2 cm)、tinggi batang (18,6-23,9 cm)、panjang pelepah (3,9-4,2 m)、直径tajuk (5,8-6,1 m)。Jumlah bunga yang terbentuk selama satu tahun pertamama pembungaan adalah 15 sampai 17 bunga per pohon。Jumlah buah muda yang bertahan hidup menurun tajampadawal, namun相对稳定setelah 2-3 bulan。Rata-rata terbentuk sebanyak 18-22 buah per tandan pada awal pembungaan dan menurun menjadi 5-6 buah per tandan saat dewasa。Buah yang bertahan hidup pada GHK (6,2 Buah / tandan) lebih tinggi diding GCK (4,0 Buah) dan GKK (4,5 Buah) pada umur 11 bulan setelah bunga mekar。拉塔-拉塔-拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔-乌拉塔。Buah kelapa GCK mempunyai代表sabut yang lebih rendah, tetapi代表tempurung dandaging, Buah yang lebih tinggi dibandingkan dengan Buah kelapa GHK dan GKK。[Kata kunci:挪威语warna buah, kelapa Genjah kopyor, kerontokan buah, kualitas buah]
{"title":"Keragaan vegetatif dan generatif pada fase pembungaan awal kelapa Genjah kopyor asal kultur embrio di Bogor, Jawa Barat","authors":"Muhammad Eko Prasetyo, Imron Riyadi, Sumaryono Sumaryono","doi":"10.22302/iribb.jur.mp.v90i1.492","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v90i1.492","url":null,"abstract":"The performance of kopyor Dwarf coconuts from embryo culture in the field has not been reported. This study was conducted to determine the difference in vegetative and generative performances of three fruit color phenotypes (brown, green, and yellow) of kopyor Dwarf coconuts in the first year after the first flower appeared. The coconuts were planted in Bogor, West Java at 260 masl with average temperature 26.9 ºC and average rainfall 4,300 mm/year. Each color phenotype was represented by 20 kopyor coconut trees from the same planting date that had not been flowering yet. When the first flowers appeared, Kopyor Brown Dwarf (KBD) had 14.6 fronds, while Kopyor Yellow Dwarf (KYD) and Kopyor Green Dwarf (KGD) had 15.5 and 17.1 fronds respectively. Other vegetative components when the first flower appeared were not significantly difference among the three color phenotypes such as stem girth (90.7-99.2 cm), stem height (18.6-23.9 cm), frond length (3.9-4.2 m) and canopy diameter (5.8-6.1 m). KBD coconuts started flowering faster than that of KGD and KYD. The numbers of flowers formed in the first year of flowering were 15 to 17 flowers per tree. There was an initial sharp decrease in the survival of young fruits, but this levelled off after 2-3 months. On average 18-22 fruits per bunch at flowering decreased to 5-6 fruits per bunch at maturity. The survival of fruits in KGD (6.2 fruits per bunch) was higher than KBD (4.0 fruits) and KYD (4.5 fruits) at 11 months after flowering. The average fruit size and weight of brown phenotype were higher than green and yellow phenotypes. The mature brown fruits had a lower husk percentage, but higher shell and fruit meat percentages than those of the green and yellow fruits.[Keywords: fruit color phenotype, kopyor Dwarf coconut, fruit abortion, fruit quality] AbstrakKeragaan kelapa Genjah kopyor asal kultur embrio di lapang belum pernah dilaporkan. Penelitian ini dilakukan untuk mengamati perbedaan keragaan vegetatif dan generatif tiga varietas kelapa Genjah kopyor yang berbeda dari warna buah (coklat, hijau dan kuning) pada tahun pertama sejak bunga pertama muncul. Kelapa tersebut ditanam di Bogor, Jawa Barat pada 260 mdpl dengan rerata suhu 26,9 ºC dan curah hujan 4.300 mm/tahun. Tiap fenotipe warna diwakili oleh 20 pohon kelapa kopyor berumur sama yang belum berbunga. Pada saat bunga pertama muncul, kelapa Genjah Coklat Kopyor (GCK) memiliki 14,6 pelepah daun, sedangkan Genjah Kuning Kopyor (GKK) dan Genjah Hijau Kopyor (GHK) berturut-turut memiliki 15,5 dan 17,1 pelepah. Parameter vegetatif lain ketika bunga pertama muncul tidak berbeda nyata antar fenotipe warna misalnya lingkar batang (90,7-99,2 cm), tinggi batang (18,6-23,9 cm), panjang pelepah (3,9-4,2 m) dan diameter tajuk (5,8-6,1 m). GCK mulai berbunga lebih cepat dibandingkan dengan GHK dan GKK. Jumlah bunga yang terbentuk selama satu tahun pertama pembungaan adalah 15 sampai 17 bunga per pohon. Jumlah buah muda yang bertahan hidup menurun tajam pada ","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89249835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.22302/iribb.jur.mp.v90i1.485
M. Gozan, Denia Apriliani Rahman, A. F. M. Harahap
AbstractLevulinic acid, a platform biochemical, might be utilized as a biodiesel additive in biodiesel production. Global demand for levulinic acids was 3,820 tons in 2020, while the roadmap target of biodiesel in Indonesia will reach 20% of diesel consumption in 2016-2025. These figures become the considerations for establishing the levulinic acid plant in Indonesia. The study's focus analyzed the economic viability of integrated levulinic acid production plant design based on Oil Palm Empty Fruit Bunches (OPEFB) in Indonesia. OPEFB was selected as raw material due to the enormous solid waste generated by oil palm plantations. In the plant design, software SuperPro Designer 9.0's used as a process tool simulator. The economic analysis showed the Net Present Value (NPV) as the US $ 548,850,764, an Internal Rate of Return (IRR) of 24.75%, and a payback period (PBP) estimated within six years with a Minimum Attractive Rate Return (MARR) of 6.1%. The optimal production capacities of levulinic acid, furfural, and formic acid are 12,425; 15,105 and 6,074 tonnes/year.[Keywords: cellulose, delignification, levulinic acid, OPEFB, simulation] AbstrakAsam levulinat, sebuah platform biokimia, dapat digunakan sebagai aditif biodiesel dalam produksi biodiesel. Permintaan global asam levulinat mencapai 3.820 ton pada 2020, sedangkan roadmap target biodiesel di Indonesia mencapai 20% dari konsumsi solar pada 2016-2025. Angka-angka ini menjadi pertimbangan untuk mendirikan pabrik asam levulinat di Indonesia. Fokus penelitian menganalisis kelayakan ekonomi rancangan pabrik produksi asam levulinat terpadu berbasis Tandan Kosong Kelapa Sawit (TKKS) di Indonesia. TKKS dipilih sebagai bahan baku karena limbah padat yang dihasilkan perkebunan kelapa sawit sangat besar. Dalam perancangan pabrik, software SuperPro Designer 9.0 digunakan sebagai alat simulator proses. Analisis ekonomi menunjukkan Net Present Value (NPV) sebesar US$ 548.850.764, Internal Rate of Return (IRR) 24,75%, dan payback period (PBP) diperkirakan dalam enam tahun dengan Minimum Attractive Rate Return (MARR) 6,1%. Kapasitas produksi optimal asam levulinat, furfural dan asam format masing-masing adalah 12.425; 15.105 dan 6.074 ton/tahun.[Kata kunci: selulosa, delignifikasi, asam levulinat, TKKS, simulasi]
摘要乙酰丙酸是一种平台生化物质，可作为生物柴油添加剂用于生物柴油生产。2020年全球乙酰丙酸需求量为3820吨，而印尼生物柴油的路线图目标是在2016-2025年达到柴油消费量的20%。这些数字成为在印尼建立乙酰丙酸厂的考虑因素。本研究重点分析了印尼油棕空果串(OPEFB)综合乙酰丙酸生产工厂设计的经济可行性。选择OPEFB作为原料是因为油棕种植园产生了大量的固体废物。在工厂设计中，使用了SuperPro Designer 9.0软件作为过程工具模拟器。经济分析显示，净现值(NPV)为548,850,764美元，内部收益率(IRR)为24.75%，投资回收期(PBP)估计为6年，最低吸引力收益率(MARR)为6.1%。乙酰丙酸、糠醛和甲酸的最佳生产能力为12425;15,105和6,074吨/年。【关键词:纤维素，去木质素作用，乙酰丙酸，OPEFB，模拟】【摘要】乙酰丙酸，油脂平台生物群落，dapat digunakan sebagai adif生物柴油;Permintaan global asam levulininapai 3.820吨/ 2020,sedangkan roadmap目标生物柴油di Indonesia menapai 20% / 2016-2025。Angka-angka ini menjadi pertimbangan untuk mendirikan pabrik asam levulinat di Indonesia。重点是在印尼，在印尼，在印尼，在印尼，在印尼，在印尼，在泰国，在印尼。kks dipilih sebagai bahan baku karena limbah padat yang dihasilkan perkebunan kelapa sawit sangat besar。Dalam perancangan pabrik，软件SuperPro Designer 9.0 digunakan sebagai模拟程序。分析经济menunjukkan净现值(NPV)为548.850.764美元，内部收益率(IRR)为24.75%，投资回收期(PBP)为最低吸引力收益率(MARR)为6.1%。Kapasitas产品最优asam去乙酰化，糠醛和asam格式的asam - masalah 12.425;15.105丹6.074吨/塔浑。[Kata kunci: selulosa, delignifikasi, asam levulinat, TKKS, simulasi]
{"title":"Techno-economic evaluation of integrated levulinic acid, formic acid, and furfural plant from oil palm empty fruit bunch with pre-treatment variations","authors":"M. Gozan, Denia Apriliani Rahman, A. F. M. Harahap","doi":"10.22302/iribb.jur.mp.v90i1.485","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v90i1.485","url":null,"abstract":"AbstractLevulinic acid, a platform biochemical, might be utilized as a biodiesel additive in biodiesel production. Global demand for levulinic acids was 3,820 tons in 2020, while the roadmap target of biodiesel in Indonesia will reach 20% of diesel consumption in 2016-2025. These figures become the considerations for establishing the levulinic acid plant in Indonesia. The study's focus analyzed the economic viability of integrated levulinic acid production plant design based on Oil Palm Empty Fruit Bunches (OPEFB) in Indonesia. OPEFB was selected as raw material due to the enormous solid waste generated by oil palm plantations. In the plant design, software SuperPro Designer 9.0's used as a process tool simulator. The economic analysis showed the Net Present Value (NPV) as the US $ 548,850,764, an Internal Rate of Return (IRR) of 24.75%, and a payback period (PBP) estimated within six years with a Minimum Attractive Rate Return (MARR) of 6.1%. The optimal production capacities of levulinic acid, furfural, and formic acid are 12,425; 15,105 and 6,074 tonnes/year.[Keywords: cellulose, delignification, levulinic acid, OPEFB, simulation] AbstrakAsam levulinat, sebuah platform biokimia, dapat digunakan sebagai aditif biodiesel dalam produksi biodiesel. Permintaan global asam levulinat mencapai 3.820 ton pada 2020, sedangkan roadmap target biodiesel di Indonesia mencapai 20% dari konsumsi solar pada 2016-2025. Angka-angka ini menjadi pertimbangan untuk mendirikan pabrik asam levulinat di Indonesia. Fokus penelitian menganalisis kelayakan ekonomi rancangan pabrik produksi asam levulinat terpadu berbasis Tandan Kosong Kelapa Sawit (TKKS) di Indonesia. TKKS dipilih sebagai bahan baku karena limbah padat yang dihasilkan perkebunan kelapa sawit sangat besar. Dalam perancangan pabrik, software SuperPro Designer 9.0 digunakan sebagai alat simulator proses. Analisis ekonomi menunjukkan Net Present Value (NPV) sebesar US$ 548.850.764, Internal Rate of Return (IRR) 24,75%, dan payback period (PBP) diperkirakan dalam enam tahun dengan Minimum Attractive Rate Return (MARR) 6,1%. Kapasitas produksi optimal asam levulinat, furfural dan asam format masing-masing adalah 12.425; 15.105 dan 6.074 ton/tahun.[Kata kunci: selulosa, delignifikasi, asam levulinat, TKKS, simulasi] ","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"46 7-8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91495385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.22302/iribb.jur.mp.v90i1.472
Agustin Sri Mulyatni, Irma Kresnawaty, Deden Dewantara Eris, Tri Panji, Wita Kimberly, H. Widiastuti, P. Priyono, Chotimah Chotimah, K. Triyana
AbstractBasal stem rot (BSR) disease caused by Ganoderma sp. is a major disease in oil palm. One of the keys to successfully control BSR disease is to detect the pathogenic infections as early as possible. Early detection technique has been developed in this study was using volatile compounds sensors known as an electronic nose, specifically Electronic Nose 118. Plant samples were obtained from roots, stems, leaves, and soils of four plant categories, which were midly, moderately, and severely infected by Ganoderma, and healthy plants. The test results showed that Electronic Nose was able to record the profile of volatile organic compounds (VOC) produced by Ganoderma sp. The Linear Discriminant Analysis (LDA) results showed that the root and stem samples were differentiated in fairly high level of discrimination with values of 89.66% and 94.59% respectively, while for internal validation value were 98.18% and 89.18%. However, for leaf and soil samples, Electronic Nose 118 resulted in low discriminations. The test results show that Electronic Nose 118 can distinguish samples of roots and stems of healthy plant and Ganoderma-infected plant with a high accuracy.[Keywords: Ganoderma, linier discriminant analysis (LDA), pathogen infection, sensor, volatile organic compound] AbstrakPenyakit busuk pangkal batang (BPB) yang disebabkan oleh Ganoderma sp. merupakan penyakit utama pada kelapa sawit. Salah satu kunci keberhasilan pengendalian penyakit BPB adalah diketahuinya infeksi patogen sedini mungkin. Teknik deteksi dini yang saat ini dikembangkan dalam penelitian ini adalah menggunakan sensor senyawa volatil yang dikenal dengan electronic nose, khususnya Electronic Nose 118. Sampel tanaman diperoleh dari akar, batang, daun, dan tanah dari empat kondisi tanaman, yaitu tanaman yang terinfeksi Ganoderma ringan, sedang, berat dan tanaman sehat. Hasil pengujian menunjukkan bahwa profil senyawa organik volatil yang dihasilkan oleh infeksi Ganoderma sp. dapat ditangkap oleh Electronic Nose 118 dengan baik. Hasil analisis Linear Discriminat Analysis (LDA) menunjukkan bahwa sampel akar dan batang terbedakan dengan tingkat diskriminasi yang cukup tinggi dengan nilai secara berurutan yaitu 89,66% dan 94,59%, sedangkan untuk validasi internal masing-masing 98,18% dan 89,18%. Namun demikian, untuk sampel daun dan tanah, pengujian dengan Electronic Nose 118 menghasilkan tingkat diskriminasi yang rendah. Hasil pengujian menunjukkan Electronic Nose 118 dapat membedakan sampel akar dan batang dari tanaman sehat dan tanaman terserang Ganoderma dengan akurasi tinggi.[Kata kunci : Ganoderma, LDA, infeksi patogen, sensor, senyawa organik volatil]
摘要由灵芝(Ganoderma sp.)引起的基茎腐病(BSR)是油棕的主要病害。尽早发现病原菌感染是成功控制BSR病的关键之一。在这项研究中开发的早期检测技术是使用一种称为电子鼻的挥发性化合物传感器，特别是电子鼻118。植物样品取自根、茎、叶和土壤4种植物类别，分别为中度、中度和重度灵芝感染植物和健康植物。检测结果表明，电子鼻能够记录灵芝挥发性有机化合物(VOC)的特征。线性判别分析(LDA)结果表明，根茎样品的鉴别水平较高，分别为89.66%和94.59%，内部验证值分别为98.18%和89.18%。然而，对于叶片和土壤样品，电子鼻118的识别率较低。试验结果表明，电子鼻118能以较高的准确度区分健康植株和灵芝感染植株的根、茎样品。[关键词]灵芝，线性判别分析(LDA)，病原菌感染，传感器，挥发性有机化合物[摘要]灵芝(Ganoderma sp. merupakan penyakit utama pada kelapa sawit。Salah satu kunci keberhasilan pengendalian penyakit BPB adalah diketahuinya infeksi病原体sedini mungkin。技术检测技术检测技术yang saat ini dikembangkan dalam penelitian ini adalah menggunakan传感器senyawa挥发性yang dikenal dengan电子鼻，khususnya电子鼻118。丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹丹哈西尔企鹅menunjukkan bahwa概况senyawa有机挥发物yang dihasilkan oleh infeksi灵芝属植物dapat ditangkap oleh电子鼻118登干baik。Hasil分析线性判别分析(LDA) menunjukkan bawa样品akar dan batang terbedakan dengan tingkat diskriminasi yang cukup tinggi dengan nilai secara berurutan yaitu 89,66%但94,59%，sedangkan untuk validasi内部masing-masing 98,18%但89,18%。Namun demikian, untuk sampel daun dan tanah，企鹅邓安电子鼻118 menghasilkan tingkat diskriminasi yang rendah。哈西尔企鹅menunjukkan电子鼻118 dapat成员达喀尔样品akar dan batang dari tanaman sehat dan tanaman terserang灵芝dengan akurasi tinggi。[Kata kunci:灵芝，LDA，真菌病原体，传感器，senyawa有机挥发物]
{"title":"Potensi electronic nose 118 untuk mendeteksi penyakit busuk pangkal batang pada kelapa sawit","authors":"Agustin Sri Mulyatni, Irma Kresnawaty, Deden Dewantara Eris, Tri Panji, Wita Kimberly, H. Widiastuti, P. Priyono, Chotimah Chotimah, K. Triyana","doi":"10.22302/iribb.jur.mp.v90i1.472","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v90i1.472","url":null,"abstract":"AbstractBasal stem rot (BSR) disease caused by Ganoderma sp. is a major disease in oil palm. One of the keys to successfully control BSR disease is to detect the pathogenic infections as early as possible. Early detection technique has been developed in this study was using volatile compounds sensors known as an electronic nose, specifically Electronic Nose 118. Plant samples were obtained from roots, stems, leaves, and soils of four plant categories, which were midly, moderately, and severely infected by Ganoderma, and healthy plants. The test results showed that Electronic Nose was able to record the profile of volatile organic compounds (VOC) produced by Ganoderma sp. The Linear Discriminant Analysis (LDA) results showed that the root and stem samples were differentiated in fairly high level of discrimination with values of 89.66% and 94.59% respectively, while for internal validation value were 98.18% and 89.18%. However, for leaf and soil samples, Electronic Nose 118 resulted in low discriminations. The test results show that Electronic Nose 118 can distinguish samples of roots and stems of healthy plant and Ganoderma-infected plant with a high accuracy.[Keywords: Ganoderma, linier discriminant analysis (LDA), pathogen infection, sensor, volatile organic compound] AbstrakPenyakit busuk pangkal batang (BPB) yang disebabkan oleh Ganoderma sp. merupakan penyakit utama pada kelapa sawit. Salah satu kunci keberhasilan pengendalian penyakit BPB adalah diketahuinya infeksi patogen sedini mungkin. Teknik deteksi dini yang saat ini dikembangkan dalam penelitian ini adalah menggunakan sensor senyawa volatil yang dikenal dengan electronic nose, khususnya Electronic Nose 118. Sampel tanaman diperoleh dari akar, batang, daun, dan tanah dari empat kondisi tanaman, yaitu tanaman yang terinfeksi Ganoderma ringan, sedang, berat dan tanaman sehat. Hasil pengujian menunjukkan bahwa profil senyawa organik volatil yang dihasilkan oleh infeksi Ganoderma sp. dapat ditangkap oleh Electronic Nose 118 dengan baik. Hasil analisis Linear Discriminat Analysis (LDA) menunjukkan bahwa sampel akar dan batang terbedakan dengan tingkat diskriminasi yang cukup tinggi dengan nilai secara berurutan yaitu 89,66% dan 94,59%, sedangkan untuk validasi internal masing-masing 98,18% dan 89,18%. Namun demikian, untuk sampel daun dan tanah, pengujian dengan Electronic Nose 118 menghasilkan tingkat diskriminasi yang rendah. Hasil pengujian menunjukkan Electronic Nose 118 dapat membedakan sampel akar dan batang dari tanaman sehat dan tanaman terserang Ganoderma dengan akurasi tinggi.[Kata kunci : Ganoderma, LDA, infeksi patogen, sensor, senyawa organik volatil]","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82653806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.22302/iribb.jur.mp.v90i1.481
Ciptadi Achmad Yusup, S. Wahyuni, Deden Dewantara Eris, P. Priyono, S. Siswanto
Abstract Chitosan has the potential as a biostimulant also as an antagonistic agent due to its antimicrobial activity. These dual benefits were the superiority of chitosan and has the potential to be developed further. The objective of this study was to analyze the effect of soluble liquid (SL) chitosan on plant growth and the yield of curly chili. The study was conducted from April-August 2019 in Prambon Subdistrict, Nganjuk District, East Java. The total experimental plot was 2.000 m2 rows with 45 x 70 cm plant spacing. The variety of curly chili used was Kribo with three treatments examined i.e., the application of single chitosan SL (P1), the combination of chitosan SL and synthetic pesticide (P2), and the conventional application of synthetic pesticide that considered as control (K). Each treatment was replicated four times. The application of chitosan SL was conducted at 21, 42, and 63 days after planting (DAP) by foliar spray. The result showed that the application of chitosan SL increased the canopy width by 16,34% and plant height by 11,53% against control at 99 DAP. The number of fruits per plant also increased by 123% on P1 and 20% on P2 treatment. The fruit weight was also increased by 42,6% on P2 and 18% on P1 treatment. The conversion result showed that single chitosan SL application was able to increase the production of curly chili per hectare up to 163%, while the combination of chitosan SL and synthetic pesticide resulted up to 71% increase in curly chili production compared to control. Chitosan has the potential as a biostimulant also as an antagonistic agent due to its antimicrobial activity. These dual benefits were the superiority of chitosan and potentially to be developed further. The objective of this study was to analyze the effect of soluble liquid (SL) chitosan on the growth and yield of curly chili. The variety of curly chili used was Kribo with three treatments examined i.e. the application of single chitosan SL (P1), the combination of chitosan SL and synthetic pesticide (P2), and the conventional application of synthetic pesticide that considered as control (K). Each treatment was replicated four times. The application of chitosan SL was conducted at 21, 42, and 63 days after planting (DAP), while the application of synthetic pesticide was conducted depending on the field conditions. The plant parameters observed were canopy width, plant height, leaf color, and number, and weight of fruits. The observation results were analyzed using table of variance. The results showed that the application of chitosan SL increased the canopy width by 16.3% and plant height by 11.5% compared to control at 99 DAP. The number of fruits per plant also increased by 123% on P1 and 20% on P2 treatment. The fruit weight was also increased by 42.6% on P2 and 18% on P1 treatment. The conversion result showed that single chitosan SL application was able to increase the yield of curly chili per hectare up to 163%, while the combination of c
摘要壳聚糖具有抗菌活性，具有作为生物刺激剂和拮抗剂的潜力。这是壳聚糖的优势，具有进一步开发的潜力。研究了壳聚糖(SL)对卷曲辣椒植株生长和产量的影响。该研究于2019年4月至8月在东爪哇省Nganjuk区Prambon街道进行。试验田总面积为行2000 m2，株距45 × 70 cm。以卷曲辣椒品种克里博为研究对象，研究了单施壳聚糖SL (P1)、壳聚糖SL与合成农药联合施用(P2)和常规施用合成农药作为对照(K) 3种处理，每种处理重复4次。分别于种植后21、42和63 d采用叶面喷施法施用壳聚糖SL。结果表明，与99 DAP对照相比，壳聚糖SL处理的冠层宽度和株高分别提高了16.34%和11.53%。单株果数在P1处理下增加123%，在P2处理下增加20%。结果表明，P2处理和P1处理的果实质量分别提高了42.6%和18%。转化结果表明，单施壳聚糖SL可使卷辣椒每公顷产量提高163%，单施壳聚糖SL与合成农药联合施用可使卷辣椒产量比对照提高71%。由于壳聚糖具有抗菌活性，它具有作为生物刺激剂和拮抗剂的潜力。这是壳聚糖的优势，具有进一步开发的潜力。研究了壳聚糖(SL)对卷曲辣椒生长和产量的影响。以卷曲辣椒品种Kribo为研究对象，采用单施壳聚糖SL (P1)、壳聚糖SL与合成农药联合施用(P2)和常规施用合成农药作为对照(K) 3种处理，每个处理重复4次。壳聚糖SL分别在种植后21、42和63 d施用，合成农药根据田间条件施用。观察到的植株参数有冠宽、株高、叶片颜色、果实数量和重量。采用方差表对观察结果进行分析。结果表明，在99 DAP处理下，壳聚糖SL处理的冠层宽度和株高分别比对照提高了16.3%和11.5%。单株果数在P1处理下增加123%，在P2处理下增加20%。果重在P2处理和P1处理下分别提高了42.6%和18%。转化结果表明，单施壳聚糖SL可使卷辣椒每公顷产量提高163%，壳聚糖SL与合成农药联合施用可使卷辣椒产量比对照提高71%。[关键词]拮抗剂，生物刺激素，克里博辣椒，生产，可溶性液体]Kedua manfaat ini yang menjadi keguulan kitosan dan berpotensi untuk dikembangkan lebih lanjut。紫檀醇可溶性液体(SL)，紫檀醇可溶液体，紫檀醇可溶液体，紫檀醇可溶液体，紫檀醇可溶液体。变种cabai keriting yang digunakan adalah Kribo dengan tiga perlakuan yang diuji, yakni applikasi kitosan SL tunggal (P1)， kombinasi kitosan SL dengan pestisida sinintetik (P2)和applikasi pestisida sinintetik secara (K). Masing-masing perlakuan diulang empat kali。1 .应用plikasi kitosan SL dilakukan padumur tanaman 21, 42和63 hari setelah tanam (HST)， sedangkan应用plakkasi pestisida sinintetik dilakukan berdasarkan kondisi di lapangan。参数tanaman yang diamati antara lain lebar kanopi, tinggi tanaman, warna dawn, serta jumlah, dan berat buah。[中文]:中国日报。Hasil penelitian menunjukkan bahwa applikasi kitosan SL mampu meningkatkan lebar kanopi dantinggi tanaman sebesar 16,3% dan11,5 % terhadap control pada umur tanaman 99 HST。Jumlah buah per tanaman juga mengalami kenaikan sebesar 123% pada perlakuan P1 20% pada perlakuan P2。Berat per buah mengalami kenaikan sebesar 42,6% padperlakuan P2和18% padperlakuan P1。Hasil konversi menunjukkan bahwa applikasi kitosan SL tunggal mampu meningkatkan produksi cabai keriting per hektar hingga 163%， sedangkan applikasi kombinasi kitosan SL dan pestilsikintetik meningkatkan produksi sebesar dibandingkan防治71%。[Kata kunci:拮抗剂，生物刺激剂，cabai var. Kribo，产品，可溶性液体]
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Pub Date : 2022-04-28DOI: 10.22302/iribb.jur.mp.v90i1.486
R. Putranto, G. Permatasari, R. T. Saptari
AbstractSugarcane (Saccharum sp.) is potential as a biofuel and biomaterial source for its high cellulose content. Cellulose is the main constituent of the plant cell wall, as a linear chain arranged in a polysaccharide bundle, called cellulose microfibril. A gene named COBRA has been revealed to play role in the orientation of microfibril and cellulose crystallization. The COBRA gene in the Saccharum spp is under-explored. Therefore, the in silico study was conducted to explore the COBRA gene in Saccharum sp. By comparative genomics methods, the COBRA genes from Arabidopsis sp. (AtCOBLs) were compared to the Saccharum sp. (SoCOBLs). The conserved domain was then identified and the cluster system was constructed under a phylogenetic tree. Furthermore, each SoCOBLs protein was modelled to analyze its structure. According to the analysis, eleven of Saccharum sp. genomic scaffolds were successfully identified. Moreover, conserved domain identification resulted in nine SoCOBLs proteins. The phylogenetic tree showed two main clusters: I and II, differentiating those COBLs families based on the protein sequence, domain motif and amino acid properties. It leads to the variation of SoCOBLs protein structure as the results of the amino acid properties. Overall, the COBRA gene has been identified genomically in Saccharum sp. Yet, the function and tissue-specific expression are still unclear. It was predicted to act as the regulator of microfibril orientation and the cellulose synthesis process. Hence, further analyses by in vitro and in vivo are indispensable.[Keywords: cellulose, comparative genomic, Saccharum sp.]AbstrakTanaman tebu (Saccharum sp.) berpotensi sebagai sumber bahan bakar nabati dan biomaterial karena kandungan selulosanya yang tinggi. Selulosa merupakan komponen utama penyusun dinding sel tanaman, sebagai rantai lurus yang tersusun dalam gugusan polisakarida, yang disebut mikrofibril selulosa. Sebuah gen bernama COBRA telah diketahui berperan dalam menentukan arah mikrofibril dan kristalisasi selulosa. Gen COBRA pada spesies Saccharum spp. belum banyak dipelajari. Oleh karena itu, kajian in silico dilakukan untuk mempelajari gen COBRA pada Saccharum sp. Melalui metode perbandingan genomika, gen COBRA dari Arabidopsis sp. (AtCOBLs) dibandingkan dengan gen COBRA dari Saccharum sp. (SoCOBLs). Domain conserve pada gen kemudian diidentifikasi dan sistem klaster disusun dalam sebuah pohon filogeni. Setelah itu, dibuat model untuk menganalisis struktur dari protein SoCOBL. Dari hasil analisis, sebelas perancah genom Saccharum sp. berhasil diidentifikasi. Kemudian, identifikasi daerah lestari menghasilkan sembilan protein SoCOBL. Pohon filogeni menggambarkan dua klaster utama: I dan II, yang membedakan famili SoCOBLs tersebut berdasarkan sekuens protein, motif domain, dan karakteristik asam amino. Karakteristik asam amino menyebabkan variasi pada struktur protein-protein SoCOBL. Secara umum, gen COBRA telah teridentifikasi pada Saccharum sp., mesk
摘要甘蔗纤维素含量高，具有作为生物燃料和生物材料的潜力。纤维素是植物细胞壁的主要成分，呈线性链排列在多糖束中，称为纤维素微原纤维。一个名为COBRA的基因被发现在微纤维取向和纤维素结晶中起作用。Saccharum属的COBRA基因尚未被充分研究。因此，我们对Saccharum sp.的COBRA基因进行了计算机研究。通过比较基因组学方法，将拟南芥sp. (AtCOBLs)的COBRA基因与Saccharum sp. (SoCOBLs)的COBRA基因进行了比较。在此基础上，通过系统发育树的构建，确定了基因的保守结构域。此外，对每个SoCOBLs蛋白进行建模以分析其结构。通过分析，成功鉴定了11个Saccharum sp.基因组支架。此外，保守结构域鉴定得到9个SoCOBLs蛋白。系统发育树显示了两个主要簇:I和II，根据蛋白质序列、结构域基序和氨基酸特性来区分COBLs家族。氨基酸性质决定了SoCOBLs蛋白结构的变化。总的来说，在Saccharum sp.中已经鉴定出COBRA基因，但其功能和组织特异性表达尚不清楚。预计它可以作为微纤维取向和纤维素合成过程的调节剂。因此，进一步的体外和体内分析是必不可少的。【关键词:纤维素，比较基因组学，Saccharum sp.】【摘要】tanaman tebu (Saccharum sp.) berpotensi sebagai sumber bahan bakar nabati dan生物材料karena kandungan selulosanya yang tinggi。Selulosa merupakan komponen utama penyusun dinding sel tanaman, sebagai rantai lurus yang tersusun dalam gugusan polisakarida, yang disebut微纤维Selulosa。细纤维与细纤维与细纤维。眼镜蛇属植物种糖蜜属。Oleh karena itu, kajian in silico dilakukan untuk mempelajari gen COBRA pada Saccharum sp. Melalui mebandingan genomics, gen COBRA dari Arabidopsis sp. (AtCOBLs) dibandingkan dengan gen COBRA dari Saccharum sp. (SoCOBLs)。域保存的pagadgen kemudian的鉴定，kaskasi系统，讨论，dalam sebuah pohon filogeni。Setelah等，建立了menganalis结构蛋白SoCOBL的模型。糖料分析，糖料属。Kemudian，鉴定了鹿茸酵母蛋白SoCOBL。Pohon filogeni menggambarkan dua klaster utama: I dan II, yang成员akan family; SoCOBLs tersebut berdasarkan sekuens蛋白，motif domain, dan karakteristik asam amino。Karakteristik asam amino menyebabkan varasi pada结构蛋白SoCOBL。真菌，真菌，玉米，玉米，玉米，玉米，玉米，玉米。Diperkirakan gen terbut berperperan sebagai pengatur是一种微纤维，它可以处理sinteslolosa。Oleh karena itu, perlu adanya分析了lebih lanjut pada在体外和体内的水平。[Kata kunci: selulosa, genomika komparatif, Saccharum sp.]
{"title":"The in silico study of the COBRA gene family in sugarcane related to potential biomass content","authors":"R. Putranto, G. Permatasari, R. T. Saptari","doi":"10.22302/iribb.jur.mp.v90i1.486","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v90i1.486","url":null,"abstract":"AbstractSugarcane (Saccharum sp.) is potential as a biofuel and biomaterial source for its high cellulose content. Cellulose is the main constituent of the plant cell wall, as a linear chain arranged in a polysaccharide bundle, called cellulose microfibril. A gene named COBRA has been revealed to play role in the orientation of microfibril and cellulose crystallization. The COBRA gene in the Saccharum spp is under-explored. Therefore, the in silico study was conducted to explore the COBRA gene in Saccharum sp. By comparative genomics methods, the COBRA genes from Arabidopsis sp. (AtCOBLs) were compared to the Saccharum sp. (SoCOBLs). The conserved domain was then identified and the cluster system was constructed under a phylogenetic tree. Furthermore, each SoCOBLs protein was modelled to analyze its structure. According to the analysis, eleven of Saccharum sp. genomic scaffolds were successfully identified. Moreover, conserved domain identification resulted in nine SoCOBLs proteins. The phylogenetic tree showed two main clusters: I and II, differentiating those COBLs families based on the protein sequence, domain motif and amino acid properties. It leads to the variation of SoCOBLs protein structure as the results of the amino acid properties. Overall, the COBRA gene has been identified genomically in Saccharum sp. Yet, the function and tissue-specific expression are still unclear. It was predicted to act as the regulator of microfibril orientation and the cellulose synthesis process. Hence, further analyses by in vitro and in vivo are indispensable.[Keywords: cellulose, comparative genomic, Saccharum sp.]AbstrakTanaman tebu (Saccharum sp.) berpotensi sebagai sumber bahan bakar nabati dan biomaterial karena kandungan selulosanya yang tinggi. Selulosa merupakan komponen utama penyusun dinding sel tanaman, sebagai rantai lurus yang tersusun dalam gugusan polisakarida, yang disebut mikrofibril selulosa. Sebuah gen bernama COBRA telah diketahui berperan dalam menentukan arah mikrofibril dan kristalisasi selulosa. Gen COBRA pada spesies Saccharum spp. belum banyak dipelajari. Oleh karena itu, kajian in silico dilakukan untuk mempelajari gen COBRA pada Saccharum sp. Melalui metode perbandingan genomika, gen COBRA dari Arabidopsis sp. (AtCOBLs) dibandingkan dengan gen COBRA dari Saccharum sp. (SoCOBLs). Domain conserve pada gen kemudian diidentifikasi dan sistem klaster disusun dalam sebuah pohon filogeni. Setelah itu, dibuat model untuk menganalisis struktur dari protein SoCOBL. Dari hasil analisis, sebelas perancah genom Saccharum sp. berhasil diidentifikasi. Kemudian, identifikasi daerah lestari menghasilkan sembilan protein SoCOBL. Pohon filogeni menggambarkan dua klaster utama: I dan II, yang membedakan famili SoCOBLs tersebut berdasarkan sekuens protein, motif domain, dan karakteristik asam amino. Karakteristik asam amino menyebabkan variasi pada struktur protein-protein SoCOBL. Secara umum, gen COBRA telah teridentifikasi pada Saccharum sp., mesk","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88278124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-28DOI: 10.22302/iribb.jur.mp.v90i1.477
Nuha Haifa Arifin, Rifki Febriansah
Abstract The SARS-CoV-2 virus is a virus that emerged in late 2019 and has yet to find a cure. On the other hand, the incidence of cervical cancer in women continues to increase along with the emergence of cases of COVID-19 caused by SARS-CoV-2. Based on WHO data in 2020 stated that there were 107 per 72,314 cancer patients infected with SARS-CoV-2. Meniran (Phyllanthus niruri L.) is a herbaceous plant in Indonesia that has secondary metabolites derived from the tannin group, such as corilagin. This compound has the potential to be developed as an antiviral and anticancer agent. Thus, the purpose of this study was to determine the potential of corilagin in meniran herbs to act as an antiviral SARS-CoV-2 and cervical anticancer compared to the drug compounds molnupiravir and paclitaxel through the STITCH & STRING bioinformatics in silico test and molecular docking method. The results of the bioinformatics test of corilagin against the SARS-CoV-2 virus showed predictions of high protein binding to AGTR2 and ENPEP with a docking score of -10.9 and -9.9 kcal/mol, respectively. Meanwhile, cervical cancer cells showed the highest predicted protein binding to IL-10 and MAPK3 with a docking score of -10.5 and -10.8 kcal/mol. The docking score of molnupiravir against the COVID-19 virus protein, AGTR2, and ENPEP were -7.4 and -7.2 kcal/mol, respectively. The docking scores of paclitaxel for IL10 and MAPK3 were -8.2 and -8.9 kcal/mol, respectively. These values indicate that the activity of corilagin with proteins AGTR2, ENPEP, IL10, and MAPK3 has stronger affinity energy than the comparison drugs molnupiravir and paclitaxel. Thus, the corilagin compound from the tannin group in meniran (Phyllanthus niruri L.) has the potential to be developed and formulated as a treatment and prevention of SARS-CoV-2 antiviral and cervical anticancer.[Keywords: corilagin, cervical cancer, Phyllanthus niruri L., SARS-CoV-2] Abstrak Virus SARS-CoV-2 merupakan virus yang muncul di akhir tahun 2019 dan belum ditemukan pengobatannya. Di sisi lain, terjadinya kasus kanker serviks pada wanita masih terus meningkat beriringan dengan munculnya kasus COVID-19 yang disebabkan oleh SARS-CoV-2. Berdasarkan data studi WHO tahun 2020 menyatakan bahwa terdapat 107 per 72.314 pasien kanker yang terinfeksi SARS-CoV-2. Meniran (Phyllanthus niruri L.) merupakan tanaman herba di Indonesia yang memiliki senyawa metabolit sekunder yang berasal dari golongan tannin seperti corilagin. Senyawa ini memiliki potensi untuk dikembangkan sebagai agen antivirus dan antikanker. Penelitian ini bertujuan untuk mengetahui potensi corilagin yang terkandung pada herba meniran sebagai antivirus SARS-CoV-2 dan antikanker serviks yang dibandingkan dengan senyawa obat molnupiravir dan paclitaxel melalui uji in silico bioinformatika STITCH & STRING serta metode molecular docking. Hasil uji bioinformatika corilagin terhadap virus SARS-CoV-2 menunjukkan prediksi pengikatan protein yang tinggi pada AGTR2 dan
SARS-CoV-2病毒是一种于2019年底出现的病毒，目前尚未找到治愈方法。另一方面，随着SARS-CoV-2感染的COVID-19病例的出现，女性宫颈癌的发病率持续上升。根据世卫组织2020年的数据，每72,314名癌症患者中有107人感染了SARS-CoV-2。Meniran (Phyllanthus niruri L.)是印度尼西亚的一种草本植物，具有源自单宁族的次级代谢产物，如corilagin。该化合物具有开发抗病毒和抗癌药物的潜力。因此，本研究的目的是通过STITCH & STRING生物信息学芯片测试和分子对接方法，与药物化合物莫努比拉韦和紫杉醇相比，确定meniran中草药中胶原蛋白抗病毒SARS-CoV-2和宫颈癌的潜力。coilagin抗SARS-CoV-2病毒的生物信息学测试结果显示，与AGTR2和ENPEP的高蛋白结合，对接评分分别为-10.9和-9.9 kcal/mol。同时，宫颈癌细胞与IL-10和MAPK3结合的预测蛋白最高，对接评分分别为-10.5和-10.8 kcal/mol。molnupiravir对COVID-19病毒蛋白、AGTR2和ENPEP的对接评分分别为-7.4和-7.2 kcal/mol。紫杉醇对IL10和MAPK3的对接评分分别为-8.2和-8.9 kcal/mol。这些值表明，胶原蛋白与AGTR2、ENPEP、IL10和MAPK3蛋白的活性比对照药物莫诺比拉韦和紫杉醇具有更强的亲和力。因此，meniran (Phyllanthus niruri L.)单宁族中的corilagin化合物具有开发和配制的潜力，可用于治疗和预防SARS-CoV-2抗病毒和子宫颈癌。【关键词】冠状病毒，子宫颈癌，毛茛，SARS-CoV-2【摘要】冠状病毒SARS-CoV-2 merupakan病毒yang muncul di akhir tahun 2019 dan belum ditemukan pengobatannya。新冠肺炎是一种新型冠状病毒，是一种新型冠状病毒，一种新型冠状病毒，一种新型冠状病毒。世卫组织数据研究表明，2020年每72.314名患者中有107人感染SARS-CoV-2。梅兰(Phyllanthus niruri L.)，梅兰(Phyllanthus niruri L.)，梅兰(merupakan tanaman herba di Indonesia)，梅兰(merupakan tanaman herba di Indonesia)Senyawa ini memiliki potential untuk dikembangkan sebagai agen抗病毒和抗结核。杨Penelitian ini bertujuan为她mengetahui potensi corilagin terkandung篇草meniran sebagai杀毒SARS-CoV-2丹antikanker serviks杨dibandingkan dengan senyawa obat molnupiravir丹紫杉醇melalui里头硅片bioinformatika针&字符串舒达metode分子对接。哈苏吉生物信息蛋白-冠状病毒SARS-CoV-2 menunjukkan prediksi pengikatan蛋白yang tinggi pada AGTR2 dan ENPEP dengan对接评分-10,9 dan -9,9 kcal/mol。Sedangkan pada sel kanker serviks menunjukkan prediksi pengikatan蛋白tertinggi pada IL-10 dan MAPK3 dengan对接评分-10,5 dan -10,8 kcal/mol。对接评分molnupiravir terhadap protein virus covid, AGTR2 dan ENPEP adalah -7,4 dan -7,2 kcal/mol。对接评分紫杉醇terhadap IL10和MAPK3 adalah -8,2和8,9 kcal/mol。研究结果表明:单克隆蛋白、活性蛋白、胶原蛋白、蛋白AGTR2、ENPEP、il - 10、MAPK3、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白、酶联蛋白。新冠病毒(SARS-CoV-2)与抗结核病毒(anti - kanker)[Kata kunci: corilagin, kanker serviks, Phyllanthus niruri L.， SARS-CoV-2]
{"title":"Uji molecular docking dan bioinformatika terhadap meniran (Phyllanthus niruri L.) sebagai antivirus SARS-CoV-2 dan antikanker serviks","authors":"Nuha Haifa Arifin, Rifki Febriansah","doi":"10.22302/iribb.jur.mp.v90i1.477","DOIUrl":"https://doi.org/10.22302/iribb.jur.mp.v90i1.477","url":null,"abstract":"Abstract The SARS-CoV-2 virus is a virus that emerged in late 2019 and has yet to find a cure. On the other hand, the incidence of cervical cancer in women continues to increase along with the emergence of cases of COVID-19 caused by SARS-CoV-2. Based on WHO data in 2020 stated that there were 107 per 72,314 cancer patients infected with SARS-CoV-2. Meniran (Phyllanthus niruri L.) is a herbaceous plant in Indonesia that has secondary metabolites derived from the tannin group, such as corilagin. This compound has the potential to be developed as an antiviral and anticancer agent. Thus, the purpose of this study was to determine the potential of corilagin in meniran herbs to act as an antiviral SARS-CoV-2 and cervical anticancer compared to the drug compounds molnupiravir and paclitaxel through the STITCH & STRING bioinformatics in silico test and molecular docking method. The results of the bioinformatics test of corilagin against the SARS-CoV-2 virus showed predictions of high protein binding to AGTR2 and ENPEP with a docking score of -10.9 and -9.9 kcal/mol, respectively. Meanwhile, cervical cancer cells showed the highest predicted protein binding to IL-10 and MAPK3 with a docking score of -10.5 and -10.8 kcal/mol. The docking score of molnupiravir against the COVID-19 virus protein, AGTR2, and ENPEP were -7.4 and -7.2 kcal/mol, respectively. The docking scores of paclitaxel for IL10 and MAPK3 were -8.2 and -8.9 kcal/mol, respectively. These values indicate that the activity of corilagin with proteins AGTR2, ENPEP, IL10, and MAPK3 has stronger affinity energy than the comparison drugs molnupiravir and paclitaxel. Thus, the corilagin compound from the tannin group in meniran (Phyllanthus niruri L.) has the potential to be developed and formulated as a treatment and prevention of SARS-CoV-2 antiviral and cervical anticancer.[Keywords: corilagin, cervical cancer, Phyllanthus niruri L., SARS-CoV-2] Abstrak Virus SARS-CoV-2 merupakan virus yang muncul di akhir tahun 2019 dan belum ditemukan pengobatannya. Di sisi lain, terjadinya kasus kanker serviks pada wanita masih terus meningkat beriringan dengan munculnya kasus COVID-19 yang disebabkan oleh SARS-CoV-2. Berdasarkan data studi WHO tahun 2020 menyatakan bahwa terdapat 107 per 72.314 pasien kanker yang terinfeksi SARS-CoV-2. Meniran (Phyllanthus niruri L.) merupakan tanaman herba di Indonesia yang memiliki senyawa metabolit sekunder yang berasal dari golongan tannin seperti corilagin. Senyawa ini memiliki potensi untuk dikembangkan sebagai agen antivirus dan antikanker. Penelitian ini bertujuan untuk mengetahui potensi corilagin yang terkandung pada herba meniran sebagai antivirus SARS-CoV-2 dan antikanker serviks yang dibandingkan dengan senyawa obat molnupiravir dan paclitaxel melalui uji in silico bioinformatika STITCH & STRING serta metode molecular docking. Hasil uji bioinformatika corilagin terhadap virus SARS-CoV-2 menunjukkan prediksi pengikatan protein yang tinggi pada AGTR2 dan ","PeriodicalId":11660,"journal":{"name":"E-Journal Menara Perkebunan","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83279098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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