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GENETIC RESPONSE OF BODY WEIGHT, SECONDARY SEXUAL CHARACTERS AND HATCHABILITY OF GIMMIZAH CHICKENS SELECTED FOR BREAST CIRCUMFERENCE 以胸围为选择标准的鸡体重、第二性征和孵化率的遗传响应
Pub Date : 2021-09-01 DOI: 10.21608/epsj.2021.195748
N. Boutrous
This experiment was conducted to study the effect of long term selection for breast circumference (BC) of Gimmizah (GM) chickens on some body measurements of males at 12, 25 and 45 weeks of age such as body weight (BW), BC, shank length (SL), and secondary sexual characters as comb length (CL) and wattle length (WL), besides fertility with hatchability and their genetic parameters. Two hundred and twenty GM hens with twenty males were randomly chosen and considered as base population (G0). Chicks produced from G0 were selected for BC at 12-wk of age and selected through additional five generations. The results revealed that selection for BC significantly increased BW at 12-wk of age for selected line compared to control one among 4 and 5 th generations. Comb length for selected lines had significantly increased compared to control lines for CL12 in G4 and G5. Wattle lengths for selected lines were increased (p˂0.05) in the last three generations compared to the rest ones among all the experimental ages. There were a significant improvement of fertility and hatchability of fertile eggs percentages among the subsequent selected generations. Most of heritability ( estimates for CL and WL were considered high and ranged between 0.50 and 0.78. Low estimates of were recorded for which ranged between 0.02 to 0.14 and for hatchability of fertile eggs between 0.02 and 0.05. Furthermore, CL represented high genetic correlation with fertility among the bird's ages and generations. In conclusion, body circumference could be used as selection tool for improving body weight and secondary sexual characters without detrimental effect on fertility, besides suggesting other body measurements as shank, comb and wattle lengths could be included in selection index.
本试验旨在研究长胸围(BC)选择对鸡12、25和45周龄雄性体重(BW)、BC、小腿长(SL)、鸡冠长(CL)和鸡冠长(WL)等身体指标以及育性、孵化率及其遗传参数的影响。随机选取转基因母鸡220只,其中雄性20只,作为基础群体(G0)。从G0生产的雏鸡在12周龄时选择BC,并通过另外五代进行选择。结果表明,在第4代和第5代中,选择BC显著增加了选择品系12周龄时的体重。在G4和G5中,CL12所选品系的梳长较对照品系显著增加。最后三代所选品系的鸡冠长度比其余各世代的鸡冠长度都增加(p小于0.05)。在以后选择的世代中,受精卵的受精率和孵化率都有显著提高。CL和WL的大部分遗传力估计被认为是高的,范围在0.50和0.78之间。记录的低估计值在0.02至0.14之间,受精卵的孵化率在0.02至0.05之间。此外,在鸟的年龄和世代之间,CL与育性具有高度的遗传相关性。综上所述,体围可以作为改善体重和第二性征的选择工具,且不会对生育造成不利影响,此外,体长、鸡冠和鸡冠等体长也可以作为选择指标。
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引用次数: 0
GENOMIC ANALYSIS OF TRANSFORMING GROWTH FACTOR β2 GENE POLYMORPHISM ASSOCIATED WITH PRODUCTIVE PERFORMANCE IN GIZA M-2 CHICKEN LINE 吉萨m-2鸡系生产性能相关转化生长因子β2基因多态性的基因组分析
Pub Date : 2021-09-01 DOI: 10.21608/epsj.2021.195783
F. Nassar
Transforming growth factor-β (TGF-β) genes considered as one of the large family of multifunctional growth factors that participate in the regulation of a broad range of biological processes in chicken. The current study was conducted to determine genetic polymorphisms of TGF-β2 gene by using PCR-RFLP method in Giza M-2 line which selected for high 6week live body weight (LBW) for seven generations compared to random bred control (RBC) line. Also, the association between TGF-β2 with productive performance was evaluated for each line. Results indicated that, Giza M-2 line had higher LBW at different ages, LBW at 6 week of age (LBW6), and carcass parts weights and percentages compared to RBC line. In addition, Giza M-2 line had high TGF-β2-BB genotype frequency value (0.80) which explains the positive effects of TGF-β2 gene on LBW and carcass traits in Giza M-2 line compared to RBC line. The current result conducts the broad significant effects of TGF-β2 gene on productive performance of chickens. In addition, the association between these valuable productive traits and TGF β2 gene will effectively develop the selection process by using MarkerAssisted Selection (MAS) in Giza M-2 line breeding program in the near future.
转化生长因子-β (TGF-β)基因被认为是参与调节鸡多种生物过程的多功能生长因子大家族之一。本研究采用PCR-RFLP方法,选取7代高6周活重(LBW)的吉萨M-2株系,与随机对照(RBC)株系比较,检测TGF-β2基因的遗传多态性。同时,对每条生产线评价TGF-β2与生产性能之间的关系。结果表明,吉萨M-2系各年龄体重、6周龄体重(LBW6)、胴体部位重量和百分比均高于RBC系。此外,吉萨M-2株系具有较高的TGF-β2- bb基因型频率值(0.80),这解释了TGF-β2基因对吉萨M-2株系的体重和胴体性状的积极影响。本研究结果揭示了TGF-β2基因对鸡生产性能的广泛显著影响。此外,这些有价值的生产性状与TGF β2基因的关联将在不久的将来利用标记辅助选择(MarkerAssisted selection, MAS)在吉萨M-2株系育种计划中有效地发展选择过程。
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引用次数: 0
IMPACT OF DIETARY ZINC OXIDE NANO-PARTICLES ON ANTIOXIDANT STATUS, LIVER AND KIDNEY FUNCTIONS IN ALEXANDRIA CHICKENS 饲粮中添加氧化锌纳米颗粒对亚历山大鸡抗氧化状态和肝肾功能的影响
Pub Date : 2021-09-01 DOI: 10.21608/epsj.2021.197911
Naela Abdel-Monem, Azza Elsebai, S. Elnagar, A. A. Abd El-hady
This study aimed to evaluate the effect of different forms of Zinc oxide as bulk and nano particles supplemented diet on hematological profile, antioxidant status, liver and kidney functions of Alexandria chickens. A total of 150 females and 25 males of Alexandria chickens at an age of 32 weeks were randomly divided into five groups in each group 30 female and 5 male for 12 weeks. The first group served as control group. Birds of the 2 nd and 3 rd groups were fed basal diet containing 40 and 80 mg zinc oxide (Bulk shape, ZnO) per kg diet and the 4 th and 5 th groups were fed basal diet containing 40 and 80 mg zinc oxide (Nano shape, ZnO-NPs) per kg diet, respectively. The results indicated that Hb and MCHC were significantly increased with the dose of 40 mg/kg ZnO. Data obtained revealed that there is a significant effect of zinc oxide forms on antioxidant status. While, 80 mg/kg ZnO-NPs caused a significant increase on catalase (CAT), superoxide dismutase (SOD) and total antioxidant capacity (TAC), but malondialdehyde (MDA) concentration was the lowest with the dose of 80 mg/kg ZnO-NPs. Total protein and albumin were affected by zinc oxide forms, sex and the interaction between them, but globulin was not significantly affected. Also, zinc oxide forms supplementation had no significant effect on liver and kidney functions. Generally, it can be considered that zinc oxide nanoparticles (ZnO-NPs) at a dose of 80 mg/kg addition to Alexandria chickens’ diet can enhance physiological and antioxidant statuses.
本试验旨在研究不同形式的氧化锌散装和纳米颗粒添加对亚历山大鸡血液学指标、抗氧化能力和肝肾功能的影响。选取32周龄的亚历山德里亚鸡150只,公鸡25只,随机分为5组,每组30只母鸡,5只公鸡,连续试验12周。第一组为对照组。第2组和第3组分别饲喂每kg氧化锌(体积型,ZnO)含量为40和80 mg的基础饲粮,第4组和第5组分别饲喂每kg氧化锌(纳米型,ZnO- nps)含量为40和80 mg的基础饲粮。结果表明,40 mg/kg氧化锌可显著提高小鼠Hb和MCHC水平。获得的数据显示,氧化锌形式对抗氧化状态有显著影响。80 mg/kg ZnO-NPs显著提高了过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和总抗氧化能力(TAC),但丙二醛(MDA)浓度以80 mg/kg ZnO-NPs最低。总蛋白和白蛋白受氧化锌形态、性别及其相互作用的影响,但对球蛋白的影响不显著。此外,补充氧化锌对肝脏和肾脏功能没有显著影响。综上所述,在亚历山大鸡饲粮中添加80 mg/kg的氧化锌纳米颗粒(ZnO-NPs)可以增强其生理和抗氧化能力。
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引用次数: 1
EFFECT OF MELITTIN AND THEPAX AS NATURAL ALTERNATIVES TO TRADITIONAL ANTIMICROBIAL AND ANTIVIRAL TREATMENTS ON BROILER PERFORMANCE AND SOME RELATED TRAITS 蜂毒素和thepax作为传统抗菌和抗病毒处理的天然替代品对肉鸡生产性能及相关性状的影响
Pub Date : 2021-09-01 DOI: 10.21608/epsj.2021.197896
mohamed ibrahim el-malky, M. Osman, Azza Elsebai, Mohamed H. Khalil, A. Safwat
The present study was performed to evaluate the untraditional natural additives (Melittin and Thepax) as prospective alternatives to classical therapy treatment through studying their effects on productive performance, carcass characteristics, meat composition and economic efficiency of broiler chicks. A total of 810 one-day old chicks, were randomly distributed into nine experimental groups of 90 birds in three replicates. The experimental treatments were: T1= Control, T2= Melittin (83.3 μg/L), T3= Melittin (166.6 μg/L), T4= Thepax (0.25 g/kg), T5= Thepax (0.5 g/kg), T6= Melittin (83.3 μg/L) + Thepax (0.25 g/kg), T7= Melittin (166.6 μg/L) + Thepax (0.25 g/kg), T8= Melittin (83.3 μg/L) + Thepax (0.5 g/kg), T9= Melittin (166.6 μg/L) + Thepax (0.5 g/kg). The results revealed that the highest BW and BWG were achieved by birds of T6 and T7 (with 8.84, 7.97% and 9.05, 8.13% higher than the control group, respectively). Moreover, birds treated with the various combinations of Thepax and Melittin (T6, T7, T8, and T9) had significantly the best FCR values (with 10.38% improvement, on average, compared to the control group). The same previous groups had significantly the lowest mortality rates. Birds of T6 recorded the highest European production efficiency factor being 376.73. Additionally, broilers treated with various combinations of Melittin and Thepax had significantly the highest carcass weight with the lowest abdominal fat percentages. It could be observed also that birds treated with higher Melittin level (T3, T7 or T9 groups) had significantly the lowest breast protein percentages and the highest fat composition. Finally, broilers of T6 obtained the best economic efficiency value. In conclusion, supplementing broilers with 83.3 μg Melittin /L water + 0.25 g Thepax /kg feed could be used to replace the classical veterinary treatments, that provides beneficial effects on productive performance, health status, and carcass quality of broilers with improving the economic efficiency of broiler production.
本试验旨在通过对肉鸡生产性能、胴体特性、肉成分和经济效益的影响,评价非传统天然添加剂(蜂毒素和Thepax)作为传统治疗的潜在替代品。试验选用日龄雏鸡810只,随机分为9个试验组,每组90只,分3个重复。试验处理为:T1=对照,T2= Melittin (83.3 μg/L), T3= Melittin (166.6 μg/L), T4= Thepax (0.25 g/kg), T5= Thepax (0.5 g/kg), T6= Melittin (83.3 μg/L) + Thepax (0.25 g/kg), T7= Melittin (166.6 μg/L) + Thepax (0.25 g/kg), T8= Melittin (83.3 μg/L) + Thepax (0.5 g/kg), T9= Melittin (166.6 μg/L) + Thepax (0.5 g/kg)。结果表明,T6和T7组的体重和体增重最高,分别比对照组高8.84、7.97%和9.05、8.13%。T6、T7、T8和T9组合处理的FCR值显著高于对照组(平均提高10.38%)。同样的前一组的死亡率明显最低。T6禽的欧洲生产效率系数最高,为376.73。此外,各组合处理的肉仔鸡胴体重最高,腹脂率最低。T3、T7和T9组蜂毒素水平越高,胸蛋白比例越低,脂肪组成越高。最终,T6期肉鸡获得最佳经济效益值。综上所述,在肉鸡饲粮中添加83.3 μg Melittin /L水+ 0.25 g Thepax /kg饲料可替代传统兽医处理,对肉鸡生产性能、健康状况和胴体品质均有有益影响,并可提高肉鸡生产经济效益。
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引用次数: 1
EFFECT OF DIETARY SUPPLEMENTATION OF LAURUS NOBILIS LEAVES MEAL ON GROWTH PERFORMANCE, CARCASS TRAITS AND BLOOD ANTIOXIDANT STATUS OF GROWING NZW RABBITS 饲粮中添加月桂叶粕对生长期NZW家兔生长性能、胴体性状和血液抗氧化状态的影响
Pub Date : 2021-09-01 DOI: 10.21608/epsj.2021.195784
I. Salim, Enayat H. Abo El-Azayem, Hanaa Bassiouni
: The present study aimed to investigate the effects of different levels of dietary supplementation of bay laurel ( Laurus nobilis L.) leaves meal (LNL) on growth performance, carcass traits, blood constituents, hematology and plasma antioxidant status of New Zealand White rabbits (NZW). Weaned New Zealand White rabbits 5 weeks old (n = 40, mean body weight 648 g) were randomly selected and classified to four dietary groups. The Control group was fed a basal diet without (LNL); the experimental groups received the basal diet with 1, 2 and 4 g LNL meal/kg diet (Groups LN1g, LN2g and LN4g, respectively). The experimental period lasted for 8 weeks. Results indicated that group LN4g rabbits recorded significantly the highest body weight at 9,11 and 13 weeks of age followed by LN2g. Also, LN4g recorded significantly the best feed conversion ratio followed by LN2g in comparison with the control group. There were no significant differences due to LNL supplementation in carcass characteristics, blood hematology, blood biochemistry and plasma antioxidant status. However, LNL supplementation numerically reduced abdominal fat, total plasma cholesterol (T.Ch), triglycerides (TG) and low-density lipoproteins (LDL) cholesterol compared to control, while, increased total protein, globulin and HDL cholesterol and the differences were not significant. Total antioxidant capacity (TAC), glutathione peroxidase activity (GPx) and catalase enzyme were not significantly affected by LNL supplementation. Generally, the results of the study revealed that bay laurel leaves were useful as a natural supplementation to promote growth of rabbits, with 4g LNL/kg diet being the best and could reduce the negative effects of summer conditions in rabbits.
本试验旨在研究饲粮中添加不同水平月桂叶粉(LNL)对新西兰大白兔(NZW)生长性能、胴体性状、血液成分、血液学及血浆抗氧化状态的影响。选取5周龄断奶新西兰大白兔40只,平均体重648 g,随机分为4个饲粮组。对照组饲喂不添加LNL的基础饲粮;试验组在基础饲粮中添加1、2和4g LNL /kg饲粮(分别为LN1g、LN2g和LN4g组)。试验期8周。结果表明,LN4g组家兔在9、11和13周龄时体重最高,LN2g组次之。与对照组相比,LN4g组饲料系数最高,其次为LN2g组。添加LNL对胴体特性、血液血液学、血液生化和血浆抗氧化状态无显著影响。然而,与对照组相比,添加LNL可以减少腹部脂肪、总血浆胆固醇(T.Ch)、甘油三酯(TG)和低密度脂蛋白(LDL)胆固醇,而增加总蛋白、球蛋白和高密度脂蛋白胆固醇,差异不显著。添加LNL对总抗氧化能力(TAC)、谷胱甘肽过氧化物酶活性(GPx)和过氧化氢酶无显著影响。综上所述,月桂叶可作为促进家兔生长的天然补充物,以4g LNL/kg日粮添加效果最佳,可减轻夏季环境对家兔的不利影响。
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引用次数: 0
EFFECT OF FEED RESTRICTION REGIMEN ON PRODUCTIVE PERFORMANCE AND ECONOMICAL EFFICIENCY OF WEANING NZW RABBITS 限饲方案对断奶NZW兔生产性能和经济效益的影响
Pub Date : 2021-09-01 DOI: 10.21608/epsj.2021.195785
S. Sherif
: This experiment aimed to study the effects of feed restriction (FR) on weaning New Zealand White Rabbit's growth performance. Seventy-five unsexed rabbits (5 weeks of age) were divided to five experimental treatments with five equal replications. Feed restriction program was used in the first two weeks (5-7 weeks old) after weaning as follows; the first treatment (control) was free fed the experimental diet. While other treatments (2 to 5) were fed restricted diets at levels of 10, 20, 30 and 40%, respectively from the free feeding of the control. All rabbits were free fed grower diet from 8 to 12 weeks of age. During this trial rabbit growth performance were evaluated, carcass traits, mortality rate and economic efficiency. Some blood parameters in rabbit serum were also determined. The obtained results illustrated that final weight of rabbit was recovered and feed conversion ratio was improved by FR. FR program did not affect carcass traits. Serum blood parameters were not affected by feed restriction. It could be concluded that feed restriction at levels of 30 or 40% during the first two weeks after weaning had beneficial effects on rabbit growth performance, improved feed conversion and economic efficiency and decreasing mortality rate.
本试验旨在研究限饲对断奶新西兰大白兔生长性能的影响。选取75只5周龄的无性家兔,分为5个实验处理,5个相同重复。断奶后前2周(5 ~ 7周龄)采用限饲方案:第一组(对照组)自由饲喂试验饲粮。其他处理(2 ~ 5)分别饲喂对照自由饲喂水平为10%、20%、30%和40%的限制性饲粮。8 ~ 12周龄自由饲喂生长饲粮。试验期间对家兔的生长性能、胴体性状、死亡率和经济效益进行评价。同时测定了兔血清的一些血液参数。结果表明,FR可恢复家兔的末重,提高饲料系数,对胴体性状无影响。血清血液参数不受饲料限制的影响。由此可见,断奶后2周限饲30%或40%有利于提高家兔生长性能,提高饲料系数和经济效益,降低死亡率。
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引用次数: 1
EFFICACY OF SUPPLEMENTATION OF PHYTASE ENZYME TO BROILER DIETS WITH OR WITHOUT DI-CALCIUM PHOSPHATE ON PERFORMANCE AND ECONOMIC APPRAISAL 添加或不添加磷酸二钙肉鸡饲粮中添加植酸酶对生产性能的影响及经济评价
Pub Date : 2021-06-01 DOI: 10.21608/epsj.2021.182510
S. Ali
One hundred and eight male one-day age broiler chicks (Ross 308) were used in a 42-day experiment with objectives to study the effect of supplementing phytase with or without added di-calcium phosphate (DCP) to broiler chickens’ diets on their performance, carcass characteristics and economic appraisal. The birds were allocated to three treatments in a completely randomized design (CRD) with three replicates each. Treatment one T1 (control), fed basal diets supplemented with + 0.1% DCP were used, treatment two T2 (basal diet supplemented with 1500 FTU/Kg phytase + 0.1% DCP) and treatment three T3 (basal diet supplemented with 1500 FTU/Kg). Results showed significant (P≤0.05) differences in feed consumption (FC), body weight (BW), body weight gain (BWG) and feed conversion ratio (FCR), where T3 recorded the best results. Some gastrointestinal tract (GIT) organs (esophagus, proventriculus, small and large intestine, liver and caeca) and abdominal fat pads were significantly (P≤0.05) different in their weights; but no significant (P≥0.05) differences in gizzards and hearts weights. There were significant (P≤0.05) differences in lengths of proventriculus, small and large intestines; no significant (P≥0.05) differences were found in lengths of esophagus and caeca. There were significant (P≤0.05) differences in meat chemical properties, (DM%, Ash%, EE%, Ca% and P%) of the breast muscle. However, no significant (P≥0.05) differences in meat sensory traits (taste, flavor color and tenderness) and mortality rates were found. Significant (P≤0.05) differences were found in tibia bone chemical and physical properties (weight, length, thickness of the medial wall, the lateral wall and the medullary canal diameter) and chemical composition of manure where T3 had the best results. The economics appraisal recorded significant (P≤0.05) differences where T3 had the least cost and highest revenue. The results reveal that using (1500FTU/Kg) phytase without adding DCP is the best for performance parameters and economic appraisal and reduced environmental pollution.
本试验以108只1日龄雄性肉仔鸡(Ross 308)为试验对象,研究在肉仔鸡饲粮中添加或不添加磷酸二钙(DCP)植酸酶对其生产性能、胴体特性和经济评价的影响。试验采用完全随机设计(CRD),每组3个重复,分为3个处理。处理1 T1(对照),饲喂添加+ 0.1% DCP的基础饲粮,处理2 T2(基础饲粮中添加1500 FTU/Kg植酸酶+ 0.1% DCP),处理3 T3(基础饲粮中添加1500 FTU/Kg)。结果表明:饲料耗食量(FC)、体重(BW)、增重(BWG)和饲料系数(FCR)差异显著(P≤0.05),其中T3表现最佳。部分胃肠道器官(食道、前脑室、小肠、大肠、肝脏和盲肠)和腹部脂肪垫重量差异显著(P≤0.05);砂囊和心脏重量差异无统计学意义(P≥0.05)。大肠、小肠、前脑室长度差异有统计学意义(P≤0.05);两组患者食道和盲肠长度差异无统计学意义(P≥0.05)。胸肌的肉化学性质(DM%、Ash%、EE%、Ca%和P%)差异显著(P≤0.05)。但肉品感官性状(口感、风味、色泽、嫩度)和死亡率无显著差异(P≥0.05)。胫骨理化性质(重量、长度、内侧壁厚度、外侧壁厚度、髓管直径)和粪便化学成分差异显著(P≤0.05),其中T3效果最好。经济评价中,T3成本最低,收益最高,差异有统计学意义(P≤0.05)。结果表明,在不添加DCP的情况下,使用(1500FTU/Kg)植酸酶可获得最佳的性能参数和经济评价,并可减少环境污染。
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引用次数: 0
انتأث رَ انمثبظ نلاخساو انمضادة نصفار ب ضَ انذخاج (IGY) عهي نمو بکت رَ اٍ الاشر شٍ اَ کولاى خم مَ عباس احمذ انمقطر *ً، محمذ عبذ انحم ذَ عمر**
Pub Date : 2021-06-01 DOI: 10.21608/epsj.2021.182744
Gameel Al-Maktari
أجريت التجربة لدراسة تأثير الأجسام المضادة (الجلوبيولينات المناعية IgY) المتخصصة المنتجة في صفار البيض ضد بکتيريا الايشريشيا کولاى حيث تم حقن دجاج بياض عند الاعمار 28 ، 30 و32 أسبوع  بمستضدات بکتيريا القولون الايشريشيا کولاى الميتة  لتحفيزها على أنتاج الأجسام المضادة المتخصصة. جمعت عينات دم من دجاج التجربة قبل وبعد عملية التحصين التجريبية. وجمع البيض المنتج لمدة أسبوعين قبل إجراء عملية التحصين وکذلک جمع البيض المنتج من دجاج التجربة بعد انتهاء عملية التحصين بالمستضدات لمدة ثلاثة أسابيع، وتم عزل الجلوبيولينات المناعية (IgY) المنتجة من صفار البيض المنتج من دجاج التجربة باستخدام طريقة تخفيف الماء. تم تقدير کمية الجلوبيولينات المناعية النقية المنتجة ودراسة تأثيرها ضد بکتيريا الايشريشيا کولاى من خلال تجربة منع النمو Growth inhibition assay)) في المختبرIn-vitro))، حيث استخدمت نفس بکتيريا الايشريشيا کولاى التي استعملت کمستضدات لتحصين دجاج التجربة. تبين من نتائج الدراسة حدوث تفاعل مناعي ايجابي بين الأجسام المضادة المنتجة الموجودة والمستضدات المحضرة من بکتيريا الايشريشيا کولاى عند إجراء اختبار التلازن (Slide Agglutination Test). وتبين أن کمية الأجسام المضادة النقية والتي تم عزلها من صفار البيض في هـذه الدراسة تراوحت بـين 0.04-0.06  جرام/ صفار، کما تبين عدم تأثير الأجسام المضادة المتخصصة المنتجة ضد بکتيريا الايشريشيا کولاى في بيئة الأوساط السائلة خلال الفترة 0- 2 ساعة من التحضين، بينما لوحظ خلال الفترة 2-4 ساعات من التحضين اختلاف في معدل نمو بکتيريا الايشريشيا المحضنة مع الأجسام المضادة  المتخصصة. ولوحظ بعد مرور 6 ساعات من التحضين أن معدل نمو بکتيريا الايشريشيا کولاى المحضنة مع الأجسام المضادة المتخصصة قد انخفض وبشکل واضح مقارنة بمعدل نمو البکتريا المتزايد بأوساط المجموعة المقارنة، إذ بلغ لوغاريتم العدد الکلى للبکتيريا cfu/ml  0.2و1.2  في الأوساط السائلة المحضنة، وذلک على التوالي. وتناقص معدل نمو بکتيريا الايشريشيا کولاى المحضنة مع الأجسام المضادة المتخصصة بحوالي 20% من معدل نمو البکتريا في المجموعة المقارنة.ولقد استنتج من الدراسة إمکانية انتاج الاجسام المضادة (IGY) المتخصصة ضد الاشريشيا کولاى من صفار بيض الدجاج المحقون في عضلة الصدر على عمر 28 أسبوع ببکتيريا الاشريشيا کولاى، وقدرة وفعالية الاجسام المضادة المتخصصة المستخلصة من صفار البيض على تثبيط نمو الاشريشيا کولاى في الأوساط السائلة في المختبر، کما يمکن ان يکون لهذه الاجسام المضادة القدرة على تخفيض استعمار بکتيريا الاشريشيا کولاى المرضية للخلايا المعوية في الدجاج. إمکانية قطاع الدواجن الاستفادة من استخدام الاجسام المضادة المنتجة في صفار بيض الدجاج في مکافحة الجراثيم المرضية المعدية والتي لا تستجيب او تقاوم بعض المضادات الحيوية. وتوصي الدراسة بعمل المزيد من التجارب لإنتاج الاجسام المضادة المتخصصة لبکتيريا الاشريشيا کولاى ودراسة قدرتها على تثبيط نمو البکتيريا على اغشية أمعاء الدجاج ودراسة إمکانية استخدامها في نطاق تجاري.
专门研究在蛋黄中生产的特殊抗体(免疫球蛋白)对凯斯凯莱的影响。在28岁、30至32周的白鸡被注射死的凯氏体胆碱酯酶抗原,以刺激它们生产专门的抗体。在试点免疫之前和之后采集了试点鸡的血液样本。生产的鸡蛋在接种前采集了两周,在三周抗原接种后采集了实验鸡的鸡蛋,并利用稀释法将实验鸡生产的蛋黄所产生的免疫球蛋白IgY)分离出来。通过在invitro实验室进行的“全氟辛酸”实验,对“全氟辛酸”(“全氟辛酸”)的抗原“抗原”)的“全氟辛酸”(“凯勒”)的抗原”(“凯勒”抗原”)进行了评价,并研究其对“凯勒”(“凯勒”)的影响。研究结果显示,现有的抗原和凯莱的抗原在平衡试验过程中发生了积极的免疫反应。在本研究中分离出来的所有纯抗体都在0.04-0.06克/ 0之间。这表明,在2- 2小时的城市化过程中,凯氏剂的特殊抗体不会在液体介质环境中产生影响,而在2-4个小时的城市化过程中,通过培养出的艾氏剂的生长速度与专门的抗体不同。经过6个小时的驯化后,凯氏菌与特制抗体的生长速度明显下降,与凯氏菌在比较群体中增长的速度相比,分别在培养的液体介质中凯凯氏菌数量的对数(cfu/ml - 0.2和1.2)。凯凯特特制抗体抗体的凯特制艾氏菌的生长速度下降了比凯特制抗体的20%。这项研究的结论是,凯斯丁·凯莱28岁大的鸡的蛋黄,以及从蛋黄中提取出来的特殊抗体能有效地阻止了凯斯在实验室液体介质中的生长。在禽类领域,我们可以利用鸡蛋蛋黄所产生的抗菌素。研究报告建议进一步研究“凯氏细菌”的特制抗体,研究它们在鸡肠膜上抑制凯氏菌生长的能力,并研究它们在商业范围内使用凯氏菌。
{"title":"انتأث رَ انمثبظ نلاخساو انمضادة نصفار ب ضَ انذخاج (IGY) عهي نمو بکت رَ اٍ الاشر شٍ اَ کولاى خم مَ عباس احمذ انمقطر *ً، محمذ عبذ انحم ذَ عمر**","authors":"Gameel Al-Maktari","doi":"10.21608/epsj.2021.182744","DOIUrl":"https://doi.org/10.21608/epsj.2021.182744","url":null,"abstract":"أجريت التجربة لدراسة تأثير الأجسام المضادة (الجلوبيولينات المناعية IgY) المتخصصة المنتجة في صفار البيض ضد بکتيريا الايشريشيا کولاى حيث تم حقن دجاج بياض عند الاعمار 28 ، 30 و32 أسبوع  بمستضدات بکتيريا القولون الايشريشيا کولاى الميتة  لتحفيزها على أنتاج الأجسام المضادة المتخصصة. جمعت عينات دم من دجاج التجربة قبل وبعد عملية التحصين التجريبية. وجمع البيض المنتج لمدة أسبوعين قبل إجراء عملية التحصين وکذلک جمع البيض المنتج من دجاج التجربة بعد انتهاء عملية التحصين بالمستضدات لمدة ثلاثة أسابيع، وتم عزل الجلوبيولينات المناعية (IgY) المنتجة من صفار البيض المنتج من دجاج التجربة باستخدام طريقة تخفيف الماء. تم تقدير کمية الجلوبيولينات المناعية النقية المنتجة ودراسة تأثيرها ضد بکتيريا الايشريشيا کولاى من خلال تجربة منع النمو Growth inhibition assay)) في المختبرIn-vitro))، حيث استخدمت نفس بکتيريا الايشريشيا کولاى التي استعملت کمستضدات لتحصين دجاج التجربة. تبين من نتائج الدراسة حدوث تفاعل مناعي ايجابي بين الأجسام المضادة المنتجة الموجودة والمستضدات المحضرة من بکتيريا الايشريشيا کولاى عند إجراء اختبار التلازن (Slide Agglutination Test). وتبين أن کمية الأجسام المضادة النقية والتي تم عزلها من صفار البيض في هـذه الدراسة تراوحت بـين 0.04-0.06  جرام/ صفار، کما تبين عدم تأثير الأجسام المضادة المتخصصة المنتجة ضد بکتيريا الايشريشيا کولاى في بيئة الأوساط السائلة خلال الفترة 0- 2 ساعة من التحضين، بينما لوحظ خلال الفترة 2-4 ساعات من التحضين اختلاف في معدل نمو بکتيريا الايشريشيا المحضنة مع الأجسام المضادة  المتخصصة. ولوحظ بعد مرور 6 ساعات من التحضين أن معدل نمو بکتيريا الايشريشيا کولاى المحضنة مع الأجسام المضادة المتخصصة قد انخفض وبشکل واضح مقارنة بمعدل نمو البکتريا المتزايد بأوساط المجموعة المقارنة، إذ بلغ لوغاريتم العدد الکلى للبکتيريا cfu/ml  0.2و1.2  في الأوساط السائلة المحضنة، وذلک على التوالي. وتناقص معدل نمو بکتيريا الايشريشيا کولاى المحضنة مع الأجسام المضادة المتخصصة بحوالي 20% من معدل نمو البکتريا في المجموعة المقارنة.ولقد استنتج من الدراسة إمکانية انتاج الاجسام المضادة (IGY) المتخصصة ضد الاشريشيا کولاى من صفار بيض الدجاج المحقون في عضلة الصدر على عمر 28 أسبوع ببکتيريا الاشريشيا کولاى، وقدرة وفعالية الاجسام المضادة المتخصصة المستخلصة من صفار البيض على تثبيط نمو الاشريشيا کولاى في الأوساط السائلة في المختبر، کما يمکن ان يکون لهذه الاجسام المضادة القدرة على تخفيض استعمار بکتيريا الاشريشيا کولاى المرضية للخلايا المعوية في الدجاج. إمکانية قطاع الدواجن الاستفادة من استخدام الاجسام المضادة المنتجة في صفار بيض الدجاج في مکافحة الجراثيم المرضية المعدية والتي لا تستجيب او تقاوم بعض المضادات الحيوية. وتوصي الدراسة بعمل المزيد من التجارب لإنتاج الاجسام المضادة المتخصصة لبکتيريا الاشريشيا کولاى ودراسة قدرتها على تثبيط نمو البکتيريا على اغشية أمعاء الدجاج ودراسة إمکانية استخدامها في نطاق تجاري.","PeriodicalId":11662,"journal":{"name":"Egyptian Poultry Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74147231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EFFECT OF DIFFERENT SEASONS ON GROWTH PERFORMANCE, IMMUNE RESPONSES AND ANTIOXIDANT STATUS OF BROILER CHICKENS 不同季节对肉鸡生长性能、免疫反应和抗氧化状态的影响
Pub Date : 2021-03-01 DOI: 10.21608/EPSJ.2021.160064
S. Youssef, M. Abdelfattah, Marwa M. Bahnas
The current experiment was carried out in farm nearby Fayoum research station to study effect of different seasons on growth performance of broiler chicks and detect early indicators for supplementing broilers flocks with antioxidant and immune enhancer. Four flocks from Arbor Acres broiler chicks were reared during summer, autumn, winter, and spring seasons in open side system. Productive performance parameters, complete blood count, antibody titers against Newcastle (NDV), antibody titers against avian influenza (H5N1) and some antioxidant parameters were measured. The following results were obtained: Body weight and body weight gain recorded significantly the highest values during spring seasons but the highest (P≤0.05) feed intake value was recorded during winter seasons and the best feed conversion value was recorded during autumn (P≤0.05). Total leukocytes count, heterophilus (H), lymphocyte (L) and H/L ratio were not affected by different seasons. Antibody titers against NDV and H5N1 record the highest significant values during summer season while the worst significant antioxidant status recorded during summer season. In contrast during winter values of antibody titers against NDV and H5N1 were significantly the worst but antioxidant parameters recorded significantly the best values. The results of the current study suggested that during winter flocks may need double doses from NDV and H5N1vaccines. However, during summer and autumn seasons, increasing antioxidant supplementation in diets or even drinking water is recommended.
本试验在法尤姆研究站附近的农场开展,旨在研究不同季节对肉仔鸡生长性能的影响,寻找在肉仔鸡群中添加抗氧化剂和免疫增强剂的早期指标。在夏、秋、冬、春4个季节分别饲养4只爱拔益加肉鸡。测定鸡的生产性能参数、全血计数、新城疫(NDV)抗体效价、禽流感(H5N1)抗体效价及部分抗氧化指标。结果表明:体重和增重在春季显著最高,采食量在冬季最高(P≤0.05),饲料系数在秋季最佳(P≤0.05)。白细胞总数、嗜白细胞数(H)、淋巴细胞数(L)和H/L比值不受季节的影响。抗NDV和H5N1抗体滴度在夏季最高,抗氧化状态在夏季最差。相反,在冬季,NDV和H5N1的抗体滴度值明显最差,而抗氧化参数值明显最好。目前的研究结果表明,在冬季,鸡群可能需要双倍剂量的新城疫和h5n1疫苗。然而,在夏季和秋季,建议在饮食甚至饮用水中增加抗氧化剂的补充。
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引用次数: 0
EFFECT OF HYDROLYSABLE TANNIN AND GRAPE SEED EXTRACT SUPPLEMENTATION ON GROWTH PERFORMANCE, ANTIOXIDATIVE STATUS AND IMMUNE RESPONSE OF GROWING RABBITS DURING SUMMER SEASON. 夏日添加水解单宁和葡萄籽提取物对生长兔生长性能、抗氧化状态和免疫反应的影响
Pub Date : 2021-03-01 DOI: 10.21608/EPSJ.2021.159930
Noha Abd El-Azeem, Hemat Abdel Magied
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引用次数: 0
期刊
Egyptian Poultry Science Journal
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