Environmental toxicology and pharmacology最新文献_第5页

Arsenic and lead exposure in vietnamese gold mining communities: A case study of metabolic enzyme impacts 越南金矿社区的砷和铅暴露：代谢酶影响的案例研究。

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-15 DOI: 10.1016/j.etap.2025.104911

Phuoc-Cuong Le , Le Van Hao , Tan Nhat , Hoang-Son Le , Thi Xuan Thuy Le

This study assesses seasonal heavy-metal contamination from gold mining in the Bong Mieu River basin (central Viet Nam) and examines its association with human xenobiotic-metabolizing enzyme phenotypes. In sediments, As and Pb consistently exceeded Vietnamese sediment quality limits, by 4.1–25.4 × for As and 2.3–7.8 × for Pb, whereas other metals generally remained within regulatory thresholds. River water exhibited widespread Pb exceedances at all sites and occasional. As exceedances, with higher concentrations in the dry season. Regression analyses indicated strong water–sediment coupling for As, Pb, Hg, Cd, and Zn, supporting shared transport and accumulation processes. Season-resolved Pearson correlations, HCA, PCA, and APCS-MLR source apportionment revealed a dominant mining-derived multi-metal signal year-round, intensified and spatially mixed under monsoon hydrology. Enzyme phenotypes varied with the mixed-metal exposure gradient: sites with higher As/Pb burdens showed higher frequencies of slow NAT2 and intermediate/slow CYP450 phenotypes, and longer residence was associated with higher NAT2 rapid-phenotype frequency.

本研究评估了Bong Mieu河流域（越南中部）金矿开采造成的季节性重金属污染，并研究了其与人类外源代谢酶表型的关系。在沉积物中，As和Pb持续超过越南沉积物质量限制，As为4.1-25.4 x， Pb为2.3-7.8 x，而其他金属总体保持在监管阈值范围内。所有站点的河水铅含量普遍超标，偶尔超标。在干旱季节浓度较高。回归分析表明，砷、铅、汞、镉和锌具有较强的水-沙耦合作用，支持共同运移和积累过程。季节分辨的Pearson相关、HCA、PCA和APCS-MLR源分析显示，在季风水文条件下，采矿衍生的多金属信号全年占主导地位，且在空间上呈增强和混合趋势。酶表型随混合金属暴露梯度的变化而变化：As/Pb负荷较高的位点显示出较高的慢NAT2和中/慢CYP450表型频率，而居住时间越长则与较高的NAT2快速表型频率相关。

{"title":"Arsenic and lead exposure in vietnamese gold mining communities: A case study of metabolic enzyme impacts","authors":"Phuoc-Cuong Le , Le Van Hao , Tan Nhat , Hoang-Son Le , Thi Xuan Thuy Le","doi":"10.1016/j.etap.2025.104911","DOIUrl":"10.1016/j.etap.2025.104911","url":null,"abstract":"<div><div>This study assesses seasonal heavy-metal contamination from gold mining in the Bong Mieu River basin (central Viet Nam) and examines its association with human xenobiotic-metabolizing enzyme phenotypes. In sediments, As and Pb consistently exceeded Vietnamese sediment quality limits, by 4.1–25.4 × for As and 2.3–7.8 × for Pb, whereas other metals generally remained within regulatory thresholds. River water exhibited widespread Pb exceedances at all sites and occasional. As exceedances, with higher concentrations in the dry season. Regression analyses indicated strong water–sediment coupling for As, Pb, Hg, Cd, and Zn, supporting shared transport and accumulation processes. Season-resolved Pearson correlations, HCA, PCA, and APCS-MLR source apportionment revealed a dominant mining-derived multi-metal signal year-round, intensified and spatially mixed under monsoon hydrology. Enzyme phenotypes varied with the mixed-metal exposure gradient: sites with higher As/Pb burdens showed higher frequencies of slow NAT2 and intermediate/slow CYP450 phenotypes, and longer residence was associated with higher NAT2 rapid-phenotype frequency.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104911"},"PeriodicalIF":4.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What lies beneath the ice? How gadolinium affects Cyclops abyssorum during Alpine winters 冰下是什么？钆对高山冬季独眼巨眼的影响。

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-15 DOI: 10.1016/j.etap.2025.104912

Alice Gabetti , Camilla Mossotto , Francesca Provenza , Serena Anselmi , Alessandra Maganza , Giuseppe Esposito , Maria Vittoria Riina , Alessandra Griglione , Stefania Squadrone , Monia Renzi , Antonia Concetta Elia , Marino Prearo , Paolo Pastorino

The increasing anthropogenic release of gadolinium (Gd) from medical contrast agents raises concern regarding its fate and ecotoxicological effects in freshwater ecosystems. Gd may reach alpine lakes through atmospheric deposition, entering environments already fragile due to extreme conditions. This study examined the sublethal effects of Gd on the alpine copepod Cyclops abyssorum under simulated winter conditions (4 °C, darkness). Organisms were exposed for 14 days to 1–150 µg/L Gd, and oxidative stress biomarkers were measured. Glutathione peroxidase activity increased with concentration, while malondialdehyde indicated lipid peroxidation. Superoxide dismutase and glutathione S-transferase showed non-linear responses, suggesting physiological thresholds or enzyme inhibition. The Threshold-based Integrative Biomarker Response (IBR-T) index revealed progressively higher stress at intermediate and high exposures. Overall, the results indicate that Gd can induce oxidative stress in cold-adapted zooplankton, emphasizing the importance of assessing contaminant risks in alpine lakes using non-model species and realistic exposure scenarios.

越来越多的人为释放的钆（Gd）从医疗造影剂引起关注其命运和生态毒理学影响的淡水生态系统。Gd可能通过大气沉积到达高山湖泊，进入由于极端条件而已经脆弱的环境。本研究研究了Gd在模拟冬季条件（4°C，黑暗）下对高山桡足动物Cyclops abyssorum的亚致死效应。将生物体暴露于1-150µg/L Gd中14天，并测量氧化应激生物标志物。谷胱甘肽过氧化物酶活性随浓度升高而升高，丙二醛表明脂质过氧化。超氧化物歧化酶和谷胱甘肽s -转移酶呈非线性反应，提示生理阈值或酶抑制。基于阈值的综合生物标志物反应（IBR-T）指数显示，在中等和高暴露下，压力逐渐增加。总体而言，研究结果表明Gd可诱导冷适应浮游动物氧化应激，强调了利用非模式物种和现实暴露情景评估高寒湖泊污染物风险的重要性。

{"title":"What lies beneath the ice? How gadolinium affects Cyclops abyssorum during Alpine winters","authors":"Alice Gabetti , Camilla Mossotto , Francesca Provenza , Serena Anselmi , Alessandra Maganza , Giuseppe Esposito , Maria Vittoria Riina , Alessandra Griglione , Stefania Squadrone , Monia Renzi , Antonia Concetta Elia , Marino Prearo , Paolo Pastorino","doi":"10.1016/j.etap.2025.104912","DOIUrl":"10.1016/j.etap.2025.104912","url":null,"abstract":"<div><div>The increasing anthropogenic release of gadolinium (Gd) from medical contrast agents raises concern regarding its fate and ecotoxicological effects in freshwater ecosystems. Gd may reach alpine lakes through atmospheric deposition, entering environments already fragile due to extreme conditions. This study examined the sublethal effects of Gd on the alpine copepod <em>Cyclops abyssorum</em> under simulated winter conditions (4 °C, darkness). Organisms were exposed for 14 days to 1–150 µg/L Gd, and oxidative stress biomarkers were measured. Glutathione peroxidase activity increased with concentration, while malondialdehyde indicated lipid peroxidation. Superoxide dismutase and glutathione S-transferase showed non-linear responses, suggesting physiological thresholds or enzyme inhibition. The Threshold-based Integrative Biomarker Response (IBR-T) index revealed progressively higher stress at intermediate and high exposures. Overall, the results indicate that Gd can induce oxidative stress in cold-adapted zooplankton, emphasizing the importance of assessing contaminant risks in alpine lakes using non-model species and realistic exposure scenarios.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104912"},"PeriodicalIF":4.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organic anion transporter 1 (OAT1) is a mechanism for uptake of mercuric-cyanide complexes into proximal tubular cells 有机阴离子转运蛋白1 （OAT1）是汞-氰化物复合物进入近端小管细胞的一种摄取机制

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-12 DOI: 10.1016/j.etap.2025.104909

Ryan Brownlee , Maria E.A. Galiciolli , Lucy Joshee , Adam M. Kiefer , Caryn S. Seney , Christy C. Bridges

The processing of gold ore with both mercury (Hg) and cyanide (^-CN) leads to widespread environmental contamination. Hg and its related compounds form complexes with ^-CN in the environment, which may result in human exposure through ingestion of contaminated food or water. The kidney is a significant site of Hg accumulation, but the mechanisms by which mercuric cyanide (Hg(CN)_n^2-n) and methylmercuric cyanide (H₃CHg(CN)_n^1-n) complexes are taken up by renal tubular cells are unknown. We hypothesized that the organic anion transporter (OAT1) is involved in the uptake of these complexes. To test this hypothesis, the time course, temperature-dependence, saturation kinetics, and substrate specificity of Hg uptake were examined in human embryonic kidney cells stably transfected with OAT1. Our data show that these complexes are taken into proximal tubular cells via OAT1. These data are the first to identify a mechanism involved in the uptake of mercuric-cyanide species into proximal tubular cells.

用汞（Hg）和氰化物（-CN）处理金矿导致了广泛的环境污染。汞及其相关化合物在环境中与-CN形成配合物，可通过摄入受污染的食物或水导致人类接触。肾脏是汞积累的重要部位，但汞氰化物（Hg(CN)n2-n）和甲基汞氰化物（H3CHg(CN)n1-n）复合物被肾小管细胞吸收的机制尚不清楚。我们假设有机阴离子转运蛋白（OAT1）参与了这些复合物的摄取。为了验证这一假设，我们在稳定转染了OAT1的人胚胎肾细胞中检测了汞摄取的时间过程、温度依赖性、饱和动力学和底物特异性。我们的数据显示，这些复合物通过OAT1进入近端小管细胞。这些数据首次确定了一种机制，涉及到汞氰化物的吸收进入近端小管细胞。

{"title":"Organic anion transporter 1 (OAT1) is a mechanism for uptake of mercuric-cyanide complexes into proximal tubular cells","authors":"Ryan Brownlee , Maria E.A. Galiciolli , Lucy Joshee , Adam M. Kiefer , Caryn S. Seney , Christy C. Bridges","doi":"10.1016/j.etap.2025.104909","DOIUrl":"10.1016/j.etap.2025.104909","url":null,"abstract":"<div><div>The processing of gold ore with both mercury (Hg) and cyanide (<sup>-</sup>CN) leads to widespread environmental contamination. Hg and its related compounds form complexes with <sup>-</sup>CN in the environment, which may result in human exposure through ingestion of contaminated food or water. The kidney is a significant site of Hg accumulation, but the mechanisms by which mercuric cyanide (Hg(CN)<sub>n</sub><sup>2-n</sup>) and methylmercuric cyanide (H<sub>3</sub>CHg(CN)<sub>n</sub><sup>1-n</sup>) complexes are taken up by renal tubular cells are unknown. We hypothesized that the organic anion transporter (OAT1) is involved in the uptake of these complexes. To test this hypothesis, the time course, temperature-dependence, saturation kinetics, and substrate specificity of Hg uptake were examined in human embryonic kidney cells stably transfected with OAT1. Our data show that these complexes are taken into proximal tubular cells via OAT1. These data are the first to identify a mechanism involved in the uptake of mercuric-cyanide species into proximal tubular cells.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104909"},"PeriodicalIF":4.2,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the effects of uracil-based pesticides on hepatic metabolic dysfunctions in low-cost three-dimensional multicellular spheroids 评估尿嘧啶类农药对低成本三维多细胞球体肝脏代谢功能障碍的影响

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-11 DOI: 10.1016/j.etap.2025.104903

Ana Caroline Pimentel de Oliveira , Letícia Ramos Molica , Karen C.M. Moraes

Pesticides are used worldwide and cause several pathologies as metabolic dysfunction-associated steatotic liver disease. Steatosis is a mark of the disease beginning; however, the mechanisms by which pesticides act on this dysfunction require further understanding. Thus, this work presents a sensitive and responsive low-cost hepatic multicellular spheroid useful to detail metabolic routes in hepatic dysfunction. Using those spheroids, the effects of pesticides bromacil and terbacil were assessed. Pesticides doses near the acceptable daily intake in Brazil changes energetic, oxidative and the one-carbon metabolism. Moreover, pesticide-specific effects were observed as increased expression of FASN, PPARs, CYP1A1, AHR, DNMTs among others, and changes in the methylation pattern of DNA, RNA and histones. Combined, the results were alarming, considering the long-lasting effects of the evaluated pesticides in agriculture fields. In addition, we can suggest that our spheroid model could be useful for large scale investigation of the effects of pesticides in liver metabolism.

农药在世界范围内广泛使用，并引起代谢功能障碍相关的脂肪变性肝病等多种病理。脂肪变性是疾病开始的标志；然而，农药作用于这种功能障碍的机制需要进一步了解。因此，这项工作提出了一个敏感和反应低成本的肝脏多细胞球体，有助于详细说明肝功能障碍的代谢途径。利用这些球体，评价了溴酸苄酯和terbacil两种农药的效果。在巴西，接近可接受日摄入量的农药剂量改变了能量、氧化和单碳代谢。此外，通过FASN、PPARs、CYP1A1、AHR、dnmt等基因的表达增加，以及DNA、RNA和组蛋白甲基化模式的改变，可以观察到农药的特异性效应。综上所述，考虑到所评估的农药在农业领域的长期影响，研究结果令人担忧。此外，我们可以建议我们的球体模型可以用于农药对肝脏代谢影响的大规模研究。

{"title":"Assessing the effects of uracil-based pesticides on hepatic metabolic dysfunctions in low-cost three-dimensional multicellular spheroids","authors":"Ana Caroline Pimentel de Oliveira , Letícia Ramos Molica , Karen C.M. Moraes","doi":"10.1016/j.etap.2025.104903","DOIUrl":"10.1016/j.etap.2025.104903","url":null,"abstract":"<div><div>Pesticides are used worldwide and cause several pathologies as metabolic dysfunction-associated steatotic liver disease. Steatosis is a mark of the disease beginning; however, the mechanisms by which pesticides act on this dysfunction require further understanding. Thus, this work presents a sensitive and responsive low-cost hepatic multicellular spheroid useful to detail metabolic routes in hepatic dysfunction. Using those spheroids, the effects of pesticides bromacil and terbacil were assessed. Pesticides doses near the acceptable daily intake in Brazil changes energetic, oxidative and the one-carbon metabolism. Moreover, pesticide-specific effects were observed as increased expression of <em>FASN</em>, <em>PPAR</em>s, <em>CYP1A1</em>, <em>AHR</em>, <em>DNMT</em>s among others, and changes in the methylation pattern of DNA, RNA and histones. Combined, the results were alarming, considering the long-lasting effects of the evaluated pesticides in agriculture fields. In addition, we can suggest that our spheroid model could be useful for large scale investigation of the effects of pesticides in liver metabolism.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104903"},"PeriodicalIF":4.2,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of per- and poly-fluoroalkyl substances (PFAS) exposure with testosterone levels in the male population 全氟烷基和多氟烷基物质（PFAS）暴露与男性人群睾酮水平的相关性

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-11 DOI: 10.1016/j.etap.2025.104906

Humaira H. Rahman , Weston R. Stokey , Stuart H. Munson-McGee

Human exposure to per- and poly-fluoroalkyl substances (PFAS) occurs from environmentally contaminated food and water. PFAS are a health concern because they are associated with various neurological, developmental, and endocrine disorders. Despite causes of infertility being equal, male causes are less studied, with etiologies ranging from genetic to anatomic to physiologic dysfunctions. This study aimed to investigate a correlation between six subtypes of PFAS with low male testosterone levels. Data from the National Health and Nutrition Examination Survey (NHANES) cycles 2013–2016 were utilized to assess serum PFAS and testosterone levels. This study observed age, marital status, body mass index, smoking status, alcohol consumption, and diabetic status being significantly correlated with low testosterone levels. PFNA exposure was observed to have a strong inverse relationship with male testosterone levels in low/medium (ORs 0.518, 0.571, 0.322, 0.455) and low/high-medium exposure levels (ORs 0.262, 0.262, 0.321, and 0.310), indicating exposure-dependent effects on testosterone levels.

人类通过受环境污染的食物和水接触到全氟烷基和多氟烷基物质。PFAS是一个健康问题，因为它们与各种神经、发育和内分泌紊乱有关。尽管不孕不育的原因是相同的，但男性原因的研究较少，其病因从遗传到解剖再到生理功能障碍。本研究旨在探讨6种PFAS亚型与低男性睾酮水平之间的相关性。利用2013-2016年国家健康与营养检查调查（NHANES）周期的数据评估血清PFAS和睾酮水平。本研究发现，年龄、婚姻状况、体重指数、吸烟状况、饮酒状况和糖尿病状况与低睾丸激素水平显著相关。PFNA暴露与男性睾酮水平在低/中等暴露水平（or值0.518,0.571,0.322,0.455）和低/高/中等暴露水平（or值0.262,0.262,0.321和0.310）呈强烈的负相关，表明暴露对睾酮水平有依赖作用。

{"title":"Correlation of per- and poly-fluoroalkyl substances (PFAS) exposure with testosterone levels in the male population","authors":"Humaira H. Rahman , Weston R. Stokey , Stuart H. Munson-McGee","doi":"10.1016/j.etap.2025.104906","DOIUrl":"10.1016/j.etap.2025.104906","url":null,"abstract":"<div><div>Human exposure to per- and poly-fluoroalkyl substances <strong>(</strong>PFAS) occurs from environmentally contaminated food and water. PFAS are a health concern because they are associated with various neurological, developmental, and endocrine disorders. Despite causes of infertility being equal, male causes are less studied, with etiologies ranging from genetic to anatomic to physiologic dysfunctions. This study aimed to investigate a correlation between six subtypes of PFAS with low male testosterone levels. Data from the National Health and Nutrition Examination Survey (NHANES) cycles 2013–2016 were utilized to assess serum PFAS and testosterone levels. This study observed age, marital status, body mass index, smoking status, alcohol consumption, and diabetic status being significantly correlated with low testosterone levels. PFNA exposure was observed to have a strong inverse relationship with male testosterone levels in low/medium (ORs 0.518, 0.571, 0.322, 0.455) and low/high-medium exposure levels (ORs 0.262, 0.262, 0.321, and 0.310), indicating exposure-dependent effects on testosterone levels.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104906"},"PeriodicalIF":4.2,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of consumer-grade and commercial acetamiprid formulations on the embryonic development of the South African clawed frog Xenopus laevis 消费级和商用醋氨虫制剂对南非爪蟾胚胎发育的影响

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-11 DOI: 10.1016/j.etap.2025.104905

Hannah Flach, Marlen Kerner, Mert Tosun, Paolina Maier, Petra Dietmann, Karin Botzenhart, Susanne J. Kühl

The insecticide acetamiprid is a neonicotinoid still approved for outdoor use worldwide. For active use, there are formulations that contain other often unpublished co-formulants in addition to acetamiprid. This study investigated effects of four different acetamiprid formulations (Careo Rosenspray, Careo, Mospilan, Danjiri) in a concentration range of 0.01–100 mg a.i./L on embryonic development of Xenopus laevis. The formulations led to an increase in embryo mortality with LC₅₀-values between 0.5 and 53.2 mg a.i./L depending on the formulation. At sublethal acetamiprid concentrations in Mospilan and Danjiri, the external appearance (body length, oedemas), the early neural development (eyes, heads, brains, cranial nerves) as well as the heart morphology and function were disturbed. Our results show that strict adherence to recommended handling and application conditions of acetamiprid formulations is essential to protect X. laevis. Therefore, pesticide risk assessment and approval renewal procedures must include commercially available combined formulations rather than solely evaluating the active ingredient.

扑热息痛是一种新烟碱类杀虫剂，在世界范围内仍被批准在室外使用。对于主动使用，除了扑热息痛外，还有含有其他通常未发表的共制剂的配方。研究了4种不同的醋氨虫制剂（Careo Rosenspray、Careo、Mospilan、Danjiri）在0.01 ~ 100 mg a.i./L浓度范围内对非洲爪蟾胚胎发育的影响。不同配方对胚胎死亡率的影响不同，lc50值在0.5 ~ 53.2 mg a.i./L之间。在Mospilan和Danjiri的亚致死浓度下，小鼠的外观（体长、水肿）、早期神经发育（眼睛、头部、大脑、脑神经）以及心脏形态和功能受到干扰。本研究结果表明，严格遵守对乙酰氨脒制剂推荐的处理和使用条件，对保护姬螨至关重要。因此，农药风险评估和批准更新程序必须包括商业上可获得的组合配方，而不是单独评估活性成分。

{"title":"Effects of consumer-grade and commercial acetamiprid formulations on the embryonic development of the South African clawed frog Xenopus laevis","authors":"Hannah Flach, Marlen Kerner, Mert Tosun, Paolina Maier, Petra Dietmann, Karin Botzenhart, Susanne J. Kühl","doi":"10.1016/j.etap.2025.104905","DOIUrl":"10.1016/j.etap.2025.104905","url":null,"abstract":"<div><div>The insecticide acetamiprid is a neonicotinoid still approved for outdoor use worldwide. For active use, there are formulations that contain other often unpublished co-formulants in addition to acetamiprid. This study investigated effects of four different acetamiprid formulations (Careo Rosenspray, Careo, Mospilan, Danjiri) in a concentration range of 0.01–100 mg a.i./L on embryonic development of <em>Xenopus laevis</em>. The formulations led to an increase in embryo mortality with LC<sub>50</sub>-values between 0.5 and 53.2 mg a.i./L depending on the formulation. At sublethal acetamiprid concentrations in Mospilan and Danjiri, the external appearance (body length, oedemas), the early neural development (eyes, heads, brains, cranial nerves) as well as the heart morphology and function were disturbed. Our results show that strict adherence to recommended handling and application conditions of acetamiprid formulations is essential to protect <em>X. laevis</em>. Therefore, pesticide risk assessment and approval renewal procedures must include commercially available combined formulations rather than solely evaluating the active ingredient.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104905"},"PeriodicalIF":4.2,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of acetamiprid on DNA methylation, transcriptomics, and neurogenesis in human mesenchymal stem cells 对乙酰脒对人间充质干细胞DNA甲基化、转录组学和神经发生的影响

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-11 DOI: 10.1016/j.etap.2025.104904

Xiaofeng Chen , Yucheng Zhu , Minghan Li , Chunyan Zhou , Fang Luo , Zechang Li , Jiangyu Zhang , Liguo Xing , Wei Liu

Acetamiprid (ACE), a Neonicotinoid insecticides (NEOs), is highly selective for insect nicotinic acetylcholine receptors (nAChRs). However, increasing evidence of hazards was reported in mammals. We investigated the effects of ACE on both global DNA methylation and epigenetic change, transcriptomic dysregulation, and neural differentiation. ACE decreased AluYb8 methylation levels in human mesenchymal stem cells. TET2 expression was enhanced suggesting that it was involved in the active DNA demethylation caused by ACE. Global DNA methylation pattern identified 87 significant differentially methylated positions (DMPs) and transcriptomic analysis identified 385 differentially expressed genes (DEGs). Pathways related to the psychiatric system, including Alzheimer's disease and TGF-β, were significantly enriched. ACE inhibited the expression of neurogenesis marker MAP2 and SNCA, which was repressed by TETs inhibitor Bobcat339. Our results suggested that exposure to ACE disturbed DNA methylation, and resulted in the impaired neural differentiation revealed by abnormal expression of neuronal and PD-related marker protein.

Acetamiprid （ACE）是一种对昆虫烟碱乙酰胆碱受体（nAChRs）具有高度选择性的新烟碱类杀虫剂。然而，在哺乳动物中报告了越来越多的危害证据。我们研究了ACE对整体DNA甲基化和表观遗传改变、转录组失调和神经分化的影响。ACE降低人间充质干细胞AluYb8甲基化水平。TET2表达增强，提示其参与ACE引起的活性DNA去甲基化。全球DNA甲基化模式鉴定出87个显著差异甲基化位点（dmp），转录组学分析鉴定出385个差异表达基因（DEGs）。与精神系统相关的通路，包括阿尔茨海默病和TGF-β，显著富集。ACE抑制神经发生标志物MAP2和SNCA的表达，而后者被TETs抑制剂Bobcat339抑制。我们的研究结果表明，暴露于ACE干扰DNA甲基化，导致神经分化受损，表现为神经元和pd相关标记蛋白的异常表达。

{"title":"Effects of acetamiprid on DNA methylation, transcriptomics, and neurogenesis in human mesenchymal stem cells","authors":"Xiaofeng Chen , Yucheng Zhu , Minghan Li , Chunyan Zhou , Fang Luo , Zechang Li , Jiangyu Zhang , Liguo Xing , Wei Liu","doi":"10.1016/j.etap.2025.104904","DOIUrl":"10.1016/j.etap.2025.104904","url":null,"abstract":"<div><div>Acetamiprid (ACE), a Neonicotinoid insecticides (NEOs), is highly selective for insect nicotinic acetylcholine receptors (nAChRs). However, increasing evidence of hazards was reported in mammals. We investigated the effects of ACE on both global DNA methylation and epigenetic change, transcriptomic dysregulation, and neural differentiation. ACE decreased AluYb8 methylation levels in human mesenchymal stem cells. TET2 expression was enhanced suggesting that it was involved in the active DNA demethylation caused by ACE. Global DNA methylation pattern identified 87 significant differentially methylated positions (DMPs) and transcriptomic analysis identified 385 differentially expressed genes (DEGs). Pathways related to the psychiatric system, including Alzheimer's disease and TGF-β, were significantly enriched. ACE inhibited the expression of neurogenesis marker MAP2 and SNCA, which was repressed by TETs inhibitor Bobcat339. Our results suggested that exposure to ACE disturbed DNA methylation, and resulted in the impaired neural differentiation revealed by abnormal expression of neuronal and PD-related marker protein.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104904"},"PeriodicalIF":4.2,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Filtered diesel exhaust via whole body exposure disrupts male reproductive function through dysregulated endocrine axis and subdued testicular GSTs in juvenile Wistar rats 过滤后的柴油废气通过全身暴露，通过内分泌轴失调和睾丸GSTs抑制破坏雄性生殖功能

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-11 DOI: 10.1016/j.etap.2025.104902

Swarnabha Sarkar , Aditya Dontham , Ravindran Revand , Abhishek Kandpal , Debabrata Dasgupta , Bahni Ray , Mayank Kumar , Asmita Patil

The aftermath of air pollution comprising diesel exhaust on male reproduction necessitates comprehensive understanding. As an extension of our previous findings, the present study was designed to investigate the role of whole body exposure to filtered fraction of diesel exhaust on the male reproductive health of juvenile Wistar rats. The rats were subjected to clean air (CAG-J)/filtered diesel exhaust (FDG-J) exposure (n = 8/group) separately in an indigenously developed whole body exposure chamber, for 6 weeks (6 h/day and 5 days/week). Results showed a significant decline testicular weights, sperm concentration, % motility, serum testosterone, LH levels in serum and testes along with testicular GSTs with alterations in histoarchitecture of testicular tissue in FDG-J. Furthermore, we observed a significant increase in % sperm morphological defects, testicular testosterone, 17 β-estradiol, and serum prolactin. These findings demonstrate that disrupted endocrine regulation and oxidant/antioxidant balance can affect testicular function in younger males.

包括柴油废气在内的空气污染对男性生殖的影响需要全面了解。作为我们之前研究结果的延伸，本研究旨在研究全身暴露于柴油废气过滤部分对幼年Wistar大鼠雄性生殖健康的作用。在自行研制的全身暴露室中分别暴露于洁净空气(CAG-J)/过滤柴油废气（FDG-J）（n = 8/组），为期6周（6 h/天和5天/周）。结果显示，FDG-J组睾丸质量、精子浓度、活动力、血清睾酮、血清和睾丸LH水平显著下降，睾丸GSTs水平显著降低，睾丸组织结构改变。此外，我们观察到精子形态缺陷、睾丸睾酮、17 β-雌二醇和血清催乳素的显著增加。这些发现表明，内分泌调节和氧化/抗氧化平衡的破坏会影响年轻男性的睾丸功能。

{"title":"Filtered diesel exhaust via whole body exposure disrupts male reproductive function through dysregulated endocrine axis and subdued testicular GSTs in juvenile Wistar rats","authors":"Swarnabha Sarkar , Aditya Dontham , Ravindran Revand , Abhishek Kandpal , Debabrata Dasgupta , Bahni Ray , Mayank Kumar , Asmita Patil","doi":"10.1016/j.etap.2025.104902","DOIUrl":"10.1016/j.etap.2025.104902","url":null,"abstract":"<div><div>The aftermath of air pollution comprising diesel exhaust on male reproduction necessitates comprehensive understanding. As an extension of our previous findings, the present study was designed to investigate the role of whole body exposure to filtered fraction of diesel exhaust on the male reproductive health of juvenile Wistar rats. The rats were subjected to clean air (CAG-J)/filtered diesel exhaust (FDG-J) exposure (n = 8/group) separately in an indigenously developed whole body exposure chamber, for 6 weeks (6 h/day and 5 days/week). Results showed a significant decline testicular weights, sperm concentration, % motility, serum testosterone, LH levels in serum and testes along with testicular GSTs with alterations in histoarchitecture of testicular tissue in FDG-J. Furthermore, we observed a significant increase in % sperm morphological defects, testicular testosterone, 17 β-estradiol, and serum prolactin. These findings demonstrate that disrupted endocrine regulation and oxidant/antioxidant balance can affect testicular function in younger males.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104902"},"PeriodicalIF":4.2,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence, compositional profiles, and human exposure of siloxanes in dust and human hair from a university campus in Hainan, China 中国海南某大学校园粉尘和头发中硅氧烷的出现、成分和人体暴露

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-10 DOI: 10.1016/j.etap.2025.104901

Wenming Chen, Xinmei Wang, Liying Wang, Yingxuan Wu, Jun Wu, Chuanbiao Xu, Jiliang Zhang

Siloxanes, common in cosmetics and personal care products, carry environmental and health risks due to persistence and bioaccumulation. This study measured 17 siloxanes in dust and human hair from a university campus in Hainan Province, China, confirming widespread contamination. In dust samples, the ƩSiloxane concentrations ranged from 58.6 to 12,359 ng/g, with ƩLinear significantly predominating over ƩCyclic. In human hair, ƩSiloxane concentrations ranged from 4.60 to 14,205 ng/g, also showing higher ƩLinear levels compared to ƩCyclic. The primary siloxanes in both matrices were L10, L9, and L11. Dust and hair siloxanes correlated significantly, identifying dust as a major source. Gender differences were observed, with female students showing higher siloxane concentrations in hair and greater exposure doses through dust ingestion (4.88 ng/kg bw/d) compared to male students (2.05 ng/kg bw/d). Given the higher exposure doses observed compared to other common organic pollutants, the potential health risks associated with siloxane exposure warrant further investigation.

硅氧烷在化妆品和个人护理产品中很常见，由于其持久性和生物蓄积性，具有环境和健康风险。这项研究测量了来自中国海南省一所大学校园的灰尘和人类头发中的17种硅氧烷，证实了污染的广泛存在。粉尘样品中ƩSiloxane浓度范围为58.6 ~ 12359 ng/g， ƩLinear显著高于ƩCyclic。在人类头发中，ƩSiloxane的浓度范围为4.60至14,205 纳克/克，ƩLinear的含量也高于ƩCyclic。两种基质中的原硅氧烷分别为L10、L9和L11。灰尘和头发硅氧烷相关性显著，确定灰尘是主要来源。观察到性别差异，与男学生（2.05 ng/kg bw/d）相比，女学生的头发中硅氧烷浓度更高，通过吸入粉尘暴露剂量也更大（4.88 ng/kg bw/d）。鉴于观察到的接触剂量高于其他常见有机污染物，与接触硅氧烷相关的潜在健康风险值得进一步调查。

{"title":"Occurrence, compositional profiles, and human exposure of siloxanes in dust and human hair from a university campus in Hainan, China","authors":"Wenming Chen, Xinmei Wang, Liying Wang, Yingxuan Wu, Jun Wu, Chuanbiao Xu, Jiliang Zhang","doi":"10.1016/j.etap.2025.104901","DOIUrl":"10.1016/j.etap.2025.104901","url":null,"abstract":"<div><div>Siloxanes, common in cosmetics and personal care products, carry environmental and health risks due to persistence and bioaccumulation. This study measured 17 siloxanes in dust and human hair from a university campus in Hainan Province, China, confirming widespread contamination. In dust samples, the ƩSiloxane concentrations ranged from 58.6 to 12,359 ng/g, with ƩLinear significantly predominating over ƩCyclic. In human hair, ƩSiloxane concentrations ranged from 4.60 to 14,205 ng/g, also showing higher ƩLinear levels compared to ƩCyclic. The primary siloxanes in both matrices were L10, L9, and L11. Dust and hair siloxanes correlated significantly, identifying dust as a major source. Gender differences were observed, with female students showing higher siloxane concentrations in hair and greater exposure doses through dust ingestion (4.88 ng/kg bw/d) compared to male students (2.05 ng/kg bw/d). Given the higher exposure doses observed compared to other common organic pollutants, the potential health risks associated with siloxane exposure warrant further investigation.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104901"},"PeriodicalIF":4.2,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary concentrations of di-butyl phthalate isomers and reproductive hormonal parameters in adult men: A systematic review with dose-response meta-analysis 成年男性尿中邻苯二甲酸二丁酯异构体浓度和生殖激素参数：一项剂量-反应荟萃分析的系统综述

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology

Pub Date : 2025-12-09 DOI: 10.1016/j.etap.2025.104900

Tiberiu M. Nita , David Vernez , Pascal Wild , Nelly Pitteloud , Nancy B. Hopf

Background

Male reproductive decline has been linked to phthalate exposure. Di-butyl phthalate isomers (DnBP and DiBP) are endocrine disruptors with inconsistent evidence regarding effects on the hypothalamic-pituitary-gonadal/thyroid (HPG/T) axis.

Objective

To perform a dose-response meta-analysis of associations between DnBP and DiBP exposure and HPG/T-axis hormones.

Methods

Five databases were searched. The associations between urinary DBP metabolite concentrations (mono-n-butyl phthalate [MnBP], mono-iso-butyl phthalate [MiBP], or nonspecific DBP metabolites [∑DBPm]) and hormone levels (TT, fT, SHBG, LH, FSH, E2, INHB, and TSH) were analyzed using meta-regressions (unadjusted [UA], covariate-adjusted [CA], fertility-adjusted [FA]).

Results

From 10,410 records, 19 studies (N = 12,128) were considered. MiBP was negatively correlated with LH (UA: β=-0.117, p = 0.02; CA: β=-0.094, p = 0.012; FA: β=-0.125, p = 0.009), positively with E2 (CA: β=0.084, p = 0.045), and SHBG (CA: β=0.09, p = 0.034; FA: β=0.091, p = 0.015). MnBP was positively associated with TSH (UA: β=0.152, p = 0.001; CA: β=0.188, p = 0.006).

Conclusions

DBP-isomers exposure is associated with altered HPG/T-axis hormone levels.

男性生殖能力下降与邻苯二甲酸盐暴露有关。邻苯二甲酸二丁酯异构体（DnBP和DiBP）是内分泌干扰物，对下丘脑-垂体-性腺/甲状腺（HPG/T）轴的影响证据不一致。目的对DnBP和DiBP暴露与HPG/ t轴激素之间的关系进行剂量-反应荟萃分析。方法对5个数据库进行检索。尿DBP代谢物浓度（邻苯二甲酸单正丁酯[MnBP]、邻苯二甲酸单异丁酯[MiBP]或非特异性DBP代谢物[∑DBPm]）与激素水平（TT、fT、SHBG、LH、FSH、E2、INHB和TSH）之间的关系采用meta回归分析（未调整[UA]、协变量调整[CA]、生育调整[FA]）。结果从10410份记录中纳入19项研究（N= 12128）。MiBP与LH呈负相关（UA: β=-0.117, p=0.02; CA: β=-0.094, p=0.012; FA: β=-0.125, p=0.009），与E2 （CA: β=0.084, p=0.045）、SHBG （CA: β=0.09, p=0.034; FA: β=0.091, p=0.015）呈正相关。MnBP与TSH呈正相关（UA: β=0.152, p=0.001; CA: β=0.188, p=0.006）。结论sdbp异构体暴露与HPG/ t轴激素水平改变有关。

{"title":"Urinary concentrations of di-butyl phthalate isomers and reproductive hormonal parameters in adult men: A systematic review with dose-response meta-analysis","authors":"Tiberiu M. Nita , David Vernez , Pascal Wild , Nelly Pitteloud , Nancy B. Hopf","doi":"10.1016/j.etap.2025.104900","DOIUrl":"10.1016/j.etap.2025.104900","url":null,"abstract":"<div><h3>Background</h3><div>Male reproductive decline has been linked to phthalate exposure. Di-butyl phthalate isomers (DnBP and DiBP) are endocrine disruptors with inconsistent evidence regarding effects on the hypothalamic-pituitary-gonadal/thyroid (HPG/T) axis.</div></div><div><h3>Objective</h3><div>To perform a dose-response meta-analysis of associations between DnBP and DiBP exposure and HPG/T-axis hormones.</div></div><div><h3>Methods</h3><div>Five databases were searched. The associations between urinary DBP metabolite concentrations (mono-n-butyl phthalate [MnBP], mono-iso-butyl phthalate [MiBP], or nonspecific DBP metabolites [∑DBPm]) and hormone levels (TT, fT, SHBG, LH, FSH, E2, INHB, and TSH) were analyzed using meta-regressions (unadjusted [UA], covariate-adjusted [CA], fertility-adjusted [FA]).</div></div><div><h3>Results</h3><div>From 10,410 records, 19 studies (N = 12,128) were considered. MiBP was negatively correlated with LH (UA: β=-0.117, p = 0.02; CA: β=-0.094, p = 0.012; FA: β=-0.125, p = 0.009), positively with E2 (CA: β=0.084, p = 0.045), and SHBG (CA: β=0.09, p = 0.034; FA: β=0.091, p = 0.015). MnBP was positively associated with TSH (UA: β=0.152, p = 0.001; CA: β=0.188, p = 0.006).</div></div><div><h3>Conclusions</h3><div>DBP-isomers exposure is associated with altered HPG/T-axis hormone levels.</div></div>","PeriodicalId":11775,"journal":{"name":"Environmental toxicology and pharmacology","volume":"121 ","pages":"Article 104900"},"PeriodicalIF":4.2,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0