Environmental toxicology and pharmacology最新文献

Microplastic (MPs) pollution has emerged as a global ecological concern, however, the impact of MPs exposure, particularly in conjunction with other pollutants such as glyphosate (GLY) on honey bee remains unknown. This study investigated the effects of exposure to different concentrations of MPs and their combination with GLY on honey bee larvae development, or during the larvae period, regulation of major detoxification, antioxidant and immune genes, and oxidative stress biomarkers. Results revealed that combined exposure to MPs and GLY decreased larvae survivorship and weight, while exposure to MPs alone showed no significant differences. Both MPs and GLY alone downregulated the defensin-1 gene, but only combined exposure with GLY downregulated the hymenoptaecin gene and increased catalase enzyme activity. The data suggest a synergistic effect of MPs and GLY, leading to immunosuppression and reduced larvae survival and weight. These findings highlight potential risks of two prevalent environmental pollutants on honey bee health.

Medicines, like food, are necessities. Many of the commonly used pharmaceuticals, especially antibiotics and NSAIDs end up in the environment and are detected in it (especially in water) at concentrations in the ng·L⁻¹- μg·L⁻¹ range. Although the concentrations of individual drugs in the environment are low, their high biological activity can cause them to be toxic to the environment. This review analyzes and summarizes the effects of drugs, primarily antibiotics and NSAIDs on photosynthesizing organisms, i.e., algae, aquatic and terrestrial plants. Acute drug toxicity to algae and plants occurs most often at high, often non-existent environmental concentrations, while sublethal effects occur at low drug concentrations. The review also points out the problems associated with ecotoxicological studies and the lack of systemic solutions to better assess the risks associated with the presence of drugs in the environment.

Artificial turf, a consumer product growing in usage in the United States, contains diverse chemicals, some of which are endocrine disruptive. Endocrine effects from turf material extracts have been primarily limited to one component, crumb rubber, of these multi-material products. We present in vitro bioactivities from non-weathered and weathered turf sample extracts, including multiple turf components. All weathered samples were collected from real-world turf fields. Non-weathered versus weathered differentially affected the androgen (AR), estrogen (ER), glucocorticoid (GR), and thyroid receptors (TR) in reporter bioassays. While weathered extracts more efficaciously activated peroxisome proliferator activated receptor γ (PPARγ), this did not translate to greater in vitro adipogenic potential. All turf extracts activated the aryl hydrocarbon receptor (AhR). High AhR-efficacy extracts induced modest rat cardiomyoblast toxicity in an AhR-dependent manner. Our data demonstrate potential endocrine and cardiometabolic effects from artificial turf material extracts, warranting further investigation into potential exposures and human health effects.

Microplastic pollution is associated with inflammation, gut dysbiosis and behavioral changes in fish. Fish have distinct personality traits but the role of personality in behavioral toxicology is rarely considered. We classified zebrafish on four behavioral axes: boldness, anxiety, sociability and exploration tendency then exposed them to low- or high- concentrations of two types of polyethylene microplastics (low- and high-density) for 28 days. Behaviors, antioxidant enzymes (catalase and superoxide dismutase), and gut microbiome were then measured. There were direct effects of microplastics on boldness, anxiety and sociability. However, fish retained their initial behavioral tendencies. Exposure to all microplastic treatments reduced average swimming speed and decreased the time spent motionless. Microplastic exposure did not affect antioxidant enzymes but did cause significant changes in the composition of the gut microbiome. This study demonstrates that environmentally realistic concentrations of microplastics can alter fish behavior, but much of the variance in response can be explained by personality.

Objective

To assess the diagnostic utility of lncRNA 51 A in detecting cognitive decline among aluminum-exposed workers occupationally.

Methods

921 male workers from an aluminum manufacturing facility underwent cognitive assessments, measurement of plasma aluminum levels and quantification of lncRNA 51 A levels. Receiver Operating Characteristic (ROC) curves were constructed to assess the diagnostic potential of lncRNA 51 A. Bayesian network model was utilized to predict the likelihood of cognitive decline among the study population.

Results

Significant differences in lncRNA 51 A levels, plasma aluminum concentration and MMSE scores were observed between cognitive normal and decline groups. The lncRNA 51 A expression was negatively correlated with MMSE scores. The area under the curve (AUC) was 0.894, with 89.3 % sensitivity and 73.9 % specificity. The Bayesian network model indicated varying probabilities of cognitive decline based on lncRNA 51 A expression levels.

Conclusion

Plasma lncRNA 51 A shows potential as an excellent biomarker for cognitive decline diagnosis in aluminum-exposed workers.

An integrated study on the levels of 7 polybrominated diphenyl ethers (PBDEs) in house dust and breast milk samples from women (N = 30) living in these households was conducted. ∑PBDEs ranged from <LOD to 523 ng g⁻¹ dust (median 4.9 ng g⁻¹ dust), and from 0.16 to 6.3 ng g⁻¹ lipid weight (lw) (median 0.49 ng g⁻¹ lw), in house dust and breast milk samples, respectively. Statistically significant correlations were observed between the mass fractions of BDE-153 from breast milk, and BDE-47, BDE-99 and BDE-100 from house dust, congeners that all originate from the “penta” formulation, indicating its dominance as PBDE exposure source in the examined household dusts. The number of electronic and electrical devices, and age of the building were positive, and the number of upholstered furniture and household area were negative predictors of PBDE dust levels. Mothers age was negative predictor of the PBDE levels in breast milk, possibly due to dilution of PBDEs in total body fat accumulated over the years. The results of health risk assessment suggest that detected PBDE levels don’t pose non-carcinogenic risk to the study population.

Heterothermic insectivorous bats are supposed to experience differential adverse effects of insecticidal pollutants depending on their seasonal and/or daily variation of metabolic and detoxification rates. Here, we investigated effects of imidacloprid, cypermethrin and fipronil on Nyctalus noctula bat-derived hepatocytes through cytotoxicity, cell inhibition and death at different concentrations (0.01, 0.1, 1, 10, 100, 1000 μg/ml), exposure times (10, 24, 48 hrs), incubation temperatures simulating hibernation (8 °C), daily torpor (20 °C), normothermy (37 °C) and active flight (40 °C), and cytochrome P450 addition. Toxic effects were significantly influenced by temperature (p < 0.05), with strong cytotoxicity after 10 hour exposure to fipronil or cypermethrin at 37 and 40 °C, cell replication inhibition (all insecticides at 8 °C) and cellular stimulation, with slight culture proliferation after 48 hours (all insecticides at 40 °C). Replacing protected chiropterans with cell cultures is a way to assess and extrapolate risks of insecticides for bats.

To determine if fluoride’s established negative impact on adult kidney health begins during gestation, an intergenerational model of Wistar rats was exposed to two doses of fluoride (2.5 or 5.0 mg/kg/day via gavage) 20 days before mating and during gestation (20 days). The results revealed that fluoride was distributed to the amniotic fluid and fetus, resulting in lower weight, more pronounced fetal restriction, and decreased creatinine, osmolarity, and amniotic fluid volume. At the kidney level, less development in the nephrogenic and cortical zones was observed in the fluoride treatment groups, with an imbalance in the number of glomeruli and "S" shaped bodies, an increase in the immunoexpression of the marker of proliferation Ki-67 in the nephrogenic zone, an increase in the expression of Wnt4 and more maturation of the renal tubules, indicating that fluoride exposure during pregnancy alters kidney development and promotes early maturation of tubular segments.

Endocrine disrupting chemicals (EDCs) may impact children's health, with medicines as a possible exposure source. Objective: to assess the potential impact of substances in paediatric medications and essential oils on children as EDC. It is a systematic review of five databases including Medline following the PECOT approach. The review focused on publications about children exposed to medication (active ingredients or excipients of interest) and having developed clinical signs of endocrine dysfunction. Out of 946 studies identified, 28 studies were included. They revealed that parabens, lavender essential oils and anti-epileptics are the most identified pharmaceutical products. The reported outcomes relate to puberty, thyroid disorders, obesity and growth. The evidence indicates potential risks, but the overall quality of available data is limited. This systematic review exposes a lack of robust evidence linking paediatric medication exposure to EDC, predominantly relying on case reports. It cautions about potential conflicts of interest.