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Production and Quality Evaluation of Gluten Free Biscuits from Maize and Soybean Flour Blends 玉米大豆粉混合无麸质饼干的生产及质量评价
Pub Date : 2023-07-25 DOI: 10.9734/ejnfs/2023/v15i81327
A. Isah, Onogwu, Oluchi Christabel, Okereke, Goodluck Obioma, Damak, Aaron Mutlong Amak
The study evaluated the quality of gluten–free biscuits made from maize and soybean flour blends, and compared them with 100% wheat flour biscuit. Flours were prepared from maize and soybean grains. The soybean flour was used to substitute 5, 10, 15, 20 and 30% of maize flour in order to obtain various flour blends. The chemical composition, functional and pasting properties of the flours and their blends were determined. The flour blends were used to prepare biscuits, which were analyzed for the chemical composition, physical and sensory properties. The results showed that the moisture, protein, fat, fiber, ash, and carbohydrate contents of the flours ranged from 10.58-11.37, 7.30-42.23, 1.82-10.15, 0.34-5.20, 0.85-4.00, 27.84-76.41%, respectively. The phosphorus, iron and magnesium contents varied from 1.24-166.96, 2.31-19.54, 0.01-157.56mg/100g, respectively. The bulk density, foaming capacity, water absorption and oil absorption capacity of the flours and the blends ranged from 0.66-0.68g/cm3, 3.22-29.25%, 85.00-210.00g/g and 80.0-145g/g, respectively. The peak viscosity, trough viscosity, breakdown viscosity, final viscosity, setback viscosity, peak time and pasting temperature decreased with an increase in the level of soybean flour incorporation and ranged from 113.05-127.98, 83.95.97.05, 23.99-41.95, 224.02-409.98, 136.01-325.03, 6.12-7.05, and 55.4-95.0 RVU, respectively. The moisture, protein, fat, fiber, ash, and carbohydrate of the biscuits increased from 4.10-9.19, 34.94-40.38, 27.24-31.39, 6.88-7.93, 0.53-3.79, and 16.99-23.44%, respectively. The phosphorus, iron, and magnesium contents of the biscuits ranged from 47.01-65.91, 3.37-7.08, 0.0018-0.0047mg/100g, respectively. The sensory scores for the biscuit samples decreased with the level of soybean flour. However, the biscuits containing 10% soybean flour was not significantly (p>0.05) different from the 100% wheat flour biscuits in all the sensory attributes evaluated. Therefore, comparable biscuits with 100% wheat biscuit could be produced from the blend of 90 % maize flour and 10% soybean flour.
本研究评价了由玉米和大豆粉混合制成的无麸质饼干的质量,并将其与100%小麦粉饼干进行了比较。面粉是由玉米和大豆谷物制成的。用大豆粉代替5%、10%、15%、20%和30%的玉米粉,得到不同比例的混合粉。测定了面粉及其共混物的化学成分、功能和糊化性能。采用混合面粉制备饼干,并对其化学成分、物理性能和感官性能进行了分析。结果表明:面粉的水分、蛋白质、脂肪、纤维、灰分和碳水化合物含量分别为10.58 ~ 11.37、7.30 ~ 42.23、1.82 ~ 10.15、0.34 ~ 5.20、0.85 ~ 4.00、27.84 ~ 76.41%。磷、铁、镁含量分别为1.24 ~ 166.96、2.31 ~ 19.54、0.01 ~ 157.56mg/100g。面粉和共混物的容重、发泡量、吸水量和吸油量分别为0.66 ~ 0.68g/cm3、3.22 ~ 29.25%、85.00 ~ 210.00g/g和80.0 ~ 145g/g。随着大豆粉掺入量的增加,浆料的峰值粘度、谷粘度、击穿粘度、终粘度、回退粘度、峰值时间和糊化温度分别为113.05 ~ 127.98、83.95.97.05、23.99 ~ 41.95、224.02 ~ 409.98、136.01 ~ 325.03、6.12 ~ 7.05和55.4 ~ 95.0 RVU。饼干的水分、蛋白质、脂肪、纤维、灰分和碳水化合物含量分别从4.10 ~ 9.19%、34.94 ~ 40.38、27.24 ~ 31.39、6.88 ~ 7.93、0.53 ~ 3.79和16.99 ~ 23.44%增加。饼干中磷、铁、镁的含量分别为47.01-65.91、3.37-7.08、0.0018-0.0047mg/100g。随着大豆粉含量的增加,饼干样品的感官分数逐渐降低。而添加10%大豆粉的饼干与添加100%小麦粉的饼干在各感官指标上均无显著差异(p>0.05)。因此,用90%的玉米粉和10%的大豆粉混合,可以生产出100%小麦饼干。
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引用次数: 0
Nutritional Fish Diseases in Aquaculture: A Human Health Hazard or Mythical Theory: An Overview 水产养殖中的营养性鱼类疾病:对人类健康的危害或神话理论:概述
Pub Date : 2023-07-21 DOI: 10.9734/ejnfs/2023/v15i81326
Fredrick Juma Syanya, Joel Anyula Litabas, W. M. Mathia, Remy Ntakirutimana
To consume or not to consume farmed fish diagnosed with nutritional disorders within an aquaculture culture system is a question that resonates with fisheries and aquaculture experts worldwide. Proper fish health management in aquaculture should be a critical area of concern for all fish farmers. It has become a fundamental requirement for achieving sustainable aquaculture production. However, many fish farmers, particularly in developing countries lack knowledge about nutritional fish diseases and how to effectively manage them on the farm. Like human health, farmed fish’s health in aquaculture systems should be a daily priority for fish farmers. Nutritional fish diseases are often overlooked by the majority of fish farmers, even at the commercial level. Most Fish farmers may not realize that nutritional disease prevention can be achieved through the use of well-formulated fish feeds that meet all the nutritional requirements for fish growth. Additionally, maintaining good water quality and implementing best management practices are essential to ensure that the fish stock remains healthy in the culture system. Since different fish species have varying nutritional dietary requirements, it is important to formulate the right nutritional diet based on the specific fish species being cultured. Fish feeds should contain the necessary nutrients to ensure opportunistic disease resistance. Furthermore, fish farmers should be familiar with early diagnosis techniques for nutritional fish disease disorders. This ensures that the harvested fish reaching the market does not raise concerns about the quality and safety of farmed fish products. Failure to diagnose nutritional disorders associated with poor feeds and diets can create doubts in the rapidly growing aquaculture industry. Therefore, this review aims to explore the nutritional requirements of farmed fish species, the impact of nutritional deficiencies on human and public health, the causes of nutritional disorders, and viable therapies for managing these diseases in farmed fish. The data for this review article were collected from various secondary sources, including internationally reputable peer-reviewed articles, conference proceedings reports, internet searches, and more. The findings highlight the importance of providing aquaculture farmers with essential information on fish nutrition-related disease control, as nutritional diseases have been found to be detrimental to fish health and public health. Moreover, many farmed fish affected by nutritional disorders have entered the global fish market, raising questions about their impact on human health. Therefore, it is crucial for fish farmers to prioritize early diagnosis of nutritional fish diseases to ensure the safety of farmed fish in the market and maintain consumer confidence in farmed fish globally. The study recommends further research into the potentially lethal effects of different nutritional fish diseases, particularly when consumed in
食用或不食用在水产养殖养殖系统中被诊断患有营养失调的养殖鱼是全世界渔业和水产养殖专家的一个共鸣问题。水产养殖中适当的鱼类健康管理应该是所有养鱼户关注的一个关键领域。它已成为实现可持续水产养殖生产的基本要求。然而,许多养鱼户,特别是发展中国家的养鱼户缺乏关于营养性鱼类疾病以及如何在养殖场有效管理这些疾病的知识。与人类健康一样,水产养殖系统中养殖鱼类的健康应成为养鱼户每天的优先事项。营养性鱼类疾病往往被大多数养鱼户所忽视,即使在商业层面也是如此。大多数养鱼户可能没有意识到,可以通过使用符合鱼类生长所有营养需求的配方良好的鱼类饲料来预防营养性疾病。此外,保持良好的水质和实施最佳管理措施对于确保养殖系统中的鱼类种群保持健康至关重要。由于不同的鱼种对营养的需求不同,因此根据所养殖的特定鱼种制定正确的营养饮食是很重要的。鱼类饲料应含有必要的营养成分,以确保对机会性疾病的抵抗力。此外,养鱼户应熟悉营养性鱼类疾病的早期诊断技术。这确保了进入市场的捕捞鱼不会引起对养殖鱼产品质量和安全的担忧。未能诊断出与不良饲料和饮食有关的营养失调,可能会给快速增长的水产养殖业带来疑虑。因此,本文旨在探讨养殖鱼类的营养需求、营养缺乏对人类和公众健康的影响、营养失调的原因以及管理这些疾病的可行治疗方法。这篇综述文章的数据收集自各种二手来源,包括国际知名的同行评议文章、会议记录报告、互联网搜索等。研究结果强调了向水产养殖户提供有关鱼类营养相关疾病控制的基本信息的重要性,因为已发现营养性疾病对鱼类健康和公众健康有害。此外,许多受营养失调影响的养殖鱼类已进入全球鱼类市场,引发了它们对人类健康影响的问题。因此,对于养鱼户来说,优先考虑营养性鱼类疾病的早期诊断,以确保市场上养殖鱼类的安全,维护全球消费者对养殖鱼类的信心至关重要。该研究建议进一步研究不同营养性鱼类疾病的潜在致命影响,特别是当大量食用时和对环境的影响,特别是当死鱼被丢弃在水生生态系统的死水中时。
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引用次数: 0
Effect of Harvesting Stage and Drying Method on Cowpea Leaf Nutrient Composition 采收阶段和干燥方式对豇豆叶片营养成分的影响
Pub Date : 2023-07-11 DOI: 10.9734/ejnfs/2023/v15i81325
Gitau Jane Wanjiku, Gathungu Geofrey Kingori, Kiramana James Kirimi
Cowpea leaves are lost annually due to infestation and spoilage when being transported to clients and the losses at the local markets are brought on by inadequate postharvest handling procedures and market glut, particularly during peak seasons. High moisture content from the cowpea leaves at harvest stage may contribute to increased spoilage hence lowering quantity and quality. The choice of appropriate harvesting stage and drying method can help to reduce this problem. The aim of this study was to determine the best harvesting stage and efficient drying method that would maintain high leaf nutrient composition. Cowpeas variety M66 was used for the research and the treatments included three harvesting stages (21, 35 and 49 days after sowing [DAS]), three drying methods (open sun, solar dryer and oven. Data was collected on iron, calcium, crude fibre, beta carotene, protein and moisture content. The data was subjected for variance using Statistical Analysis System 9.2 edition and significantly different means separated using least significant difference at 5%. The results indicated significant (p<0.05) differences in moisture, calcium, proteins, beta carotene and crude fibre content in both trials. Harvest stage and drying method did not significantly (p<0.05) influence the iron content. Oven and solar drying methods showed better nutrient and mineral retention in the three harvesting stages when compared to the open sun drying method.
豇豆叶每年都因在运往客户的过程中受到虫害和变质而损失,而当地市场的损失是由于采后处理程序不充分和市场供过于求造成的,特别是在旺季。收获阶段豇豆叶片的高水分含量可能导致腐坏,从而降低数量和质量。选择合适的采收阶段和干燥方法有助于减少这一问题。本研究的目的是确定最佳采收期和有效的干燥方法,以保持较高的叶片营养成分。以豇豆品种M66为研究对象,分3个采收阶段(播后21、35和49 d)和3种干燥方式(露天、太阳能干燥器和烘箱)。收集了铁、钙、粗纤维、β -胡萝卜素、蛋白质和水分含量的数据。采用统计分析系统9.2版对数据进行方差分析,采用5%的最小显著差异对显著差异均值进行分离。结果表明,两个试验的水分、钙、蛋白质、β -胡萝卜素和粗纤维含量差异显著(p<0.05)。采收阶段和干燥方式对铁含量影响不显著(p<0.05)。与露天晒干相比,烘箱晒干和日光晒干在三个收获阶段均能更好地保留养分和矿物质。
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引用次数: 0
Principles and Practices of Effective Food Safety Risk Communication – Introducing the Asia-Pacific Economic Cooperation Food Safety Risk Communication Framework 有效的食品安全风险沟通原则与实践-介绍亚太经济合作组织食品安全风险沟通框架
Pub Date : 2023-07-01 DOI: 10.9734/ejnfs/2023/v15i81324
Hongki Jin, L. Adewoye, E. Dupouy, Jun Cheng Er, Frank Geraghty, W. Hallman, M. Healy, Yifan Jiang, Elizabeth de Leon-Lim, C. Narrod, Amy Philpott
This paper describes the principles and practices of effective food safety risk communication developed under the “Asia-Pacific Economic Cooperation Food Safety Risk Communication Framework and Associated Guidelines”. The framework recommends that effective food safety risk communication needs to be centered on the clear goal of protecting consumer health by ensuring transparency and timeliness, and communication on food safety matters should be conducted in a two-way process. It emphasizes the need to provide the public with credible information based on science and evidence and highlights that food safety is a shared responsibility among all stakeholders, including industry, government agencies, media organizations and consumers. It further highlights that food safety risk communication needs to be conducted in a consistent, systematic, inclusive, consultative, and preventative manner. The framework also indicates that effective food safety risk communication needs to pay attention to the backgrounds, experiences, and needs of concerned audiences. It stresses the importance of continuous improvement of the communication system to ensure food safety risk communication to be effective. Focusing on effective communications in response to food safety incidents, emergency or crisis situations, as well as everyday food safety communications, these principles and practical guidelines will help food safety regulators, food industry, food safety educators and other stakeholders improve their communications with target audiences.
本文描述了在“亚太经合组织食品安全风险沟通框架及相关指南”下制定的有效食品安全风险沟通的原则和实践。该框架建议,有效的食品安全风险沟通需要以保护消费者健康的明确目标为中心,通过确保透明度和及时性,并在双向过程中进行有关食品安全事项的沟通。它强调需要向公众提供基于科学和证据的可靠信息,并强调食品安全是所有利益攸关方(包括行业、政府机构、媒体组织和消费者)的共同责任。它进一步强调,食品安全风险沟通需要以一致、系统、包容、协商和预防的方式进行。该框架还表明,有效的食品安全风险沟通需要关注相关受众的背景、经验和需求。它强调了持续改进沟通体系以确保食品安全风险沟通有效的重要性。这些原则和实用指南侧重于应对食品安全事件、紧急或危机情况以及日常食品安全沟通的有效沟通,将有助于食品安全监管机构、食品行业、食品安全教育工作者和其他利益攸关方改善与目标受众的沟通。
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引用次数: 0
Nutritional Status and Nutrient Adequacy of Food Consumed by Commercial Drivers in Abeokuta South Local Govt Area, Ogun State, Nigeria 尼日利亚奥贡州阿贝奥库塔南部地方政府区商业司机所消费食物的营养状况和营养充足性
Pub Date : 2023-06-29 DOI: 10.9734/ejnfs/2023/v15i81323
Opeyemi S. Oyesanya, G. Oladosu, Emmanuel Ayotunde Akinlose, O. Bolajoko, O. Onabanjo
Introduction: The nutritional status and adequacy of nutrients in the food consumed by commercial drivers have significant implications for their overall health, well-being, and job performance. Aims: The study assessed the nutritional status and nutrient adequacy of food consumed by commercial drivers in Abeokuta South Local Government area, Ogun State. Study Design: A descriptive cross-sectional design was used to carry out this study. Place and Duration of Study: The study was carried out in the Major Parks of Abeokuta South Local Government from November 2019 to February 2020. Methodology: The data was obtained from randomly selected three hundred (300) commercial drivers in the major parks (Asero, Ijaiye, Kuti, and Sapon) of Abeokuta South Local Government. A structured-interviewer-administered questionnaire was used to obtain data on the respondent’s demographics and socioeconomic characteristics, anthropometrics characteristics were assessed using a standardized method by trained anthropometrists, and the dietary intake of the respondents was obtained using a validated 24-hour recall questionnaire. Data were analyzed and presented using descriptive and inferential statistics. Results: All (100%) of the respondents were males with a mean age of 38.7±0.49 years. The mean height and weight of the respondents were found to be 1.68±0.86 m and 68.50±8.47 kg respectively. More than half (59.7%) of the respondents had a normal BMI, 34.3% and 0.3% were overweight and obese respectively and only a few (5.7%) were underweight. The nutrient adequacy results revealed inadequacies of Vitamin C (83.3%), Vitamin B1 (66.7%), Vitamin B6 (79.3%), Calcium (89.3%), Zinc (53.3%), and Magnesium (70.7%). Conclusion: It is concluded that overweight, obesity, and micronutrients inadequacy is prevalent among commercial drivers. Malnutrition and micronutrient inadequacy among commercial drivers should be recognized as a public health problem and strategies to improve their status and nutrient intake should be implemented.
导读:商业司机所消费食物的营养状况和营养充足性对他们的整体健康、福祉和工作表现有重要影响。目的:该研究评估了奥贡州阿贝奥库塔南部地方政府地区商业司机消费的食物的营养状况和营养充足性。研究设计:采用描述性横断面设计进行本研究。研究地点和时间:该研究于2019年11月至2020年2月在阿贝奥库塔南部地方政府的主要公园进行。方法:数据是从阿贝奥库塔南部地方政府主要公园(Asero、Ijaiye、Kuti和Sapon)随机抽取的300名商业司机中获得的。采用结构化访谈者管理的问卷来获取受访者的人口统计学和社会经济特征数据,由训练有素的人体测量学家使用标准化方法评估人体测量特征,并使用经过验证的24小时召回问卷获得受访者的饮食摄入量。使用描述性和推理统计对数据进行分析和呈现。结果:全部(100%)为男性,平均年龄38.7±0.49岁。调查对象的平均身高为1.68±0.86 m,体重为68.50±8.47 kg。超过一半(59.7%)的受访者BMI正常,34.3%的人超重,0.3%的人肥胖,只有少数(5.7%)的人体重不足。维生素C(83.3%)、维生素B1(66.7%)、维生素B6(79.3%)、钙(89.3%)、锌(53.3%)、镁(70.7%)缺乏。结论:商业司机中存在超重、肥胖和微量营养素缺乏的普遍现象。应认识到商业司机的营养不良和微量营养素不足是一个公共卫生问题,并应实施改善其状况和营养摄入的战略。
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引用次数: 0
Willingness to Pay for Centralized Modern Locust Bean Processing Equipment in Ekiti State, Nigeria 尼日利亚埃基蒂州购买集中式现代刺槐豆加工设备的意愿
Pub Date : 2023-06-27 DOI: 10.9734/ejnfs/2023/v15i71321
K. A. Abdu-Raheem, F. Oluwatusin, A. Kolawole, A. O. Adekunmi, O. Ajewole, F. Osundare
This study investigated the propensity to pay for modern apparatus processing locust beans in Ekiti State, Nigeria. Utilizing primary data and a multistage sampling strategy, 240 processors were chosen for the study. The collected data were analyzed utilizing descriptive statistics and an inferential statistical instrument, such as Logit and ordinary least square regression analysis. About 72 percent of processors were married and had an average of eight years of experience processing locust legumes. The majority of respondents belonged to a cooperative group (66.7%) and had no access to credit (100%). The average monthly income from processing was N18,000. The majority of respondents (65%) were unwilling to pay for the service of centralized, advanced or contemporary processing equipment. According to the results of the Logit regression analysis, the variables that significantly influence willingness to pay for the service of centralized modern locust bean processing equipment were household income and processing experience (1%), level of education and age (5%), and household size (10%). The Ordinary Least Square (OLS) analysis reveals that household size and education level were significant at the 1% level, while age and marital status were significant at the 5% level and processing experience was significant at the 1% level. The study suggests that processors ready to pay should form a formidable cooperative to secure grants or loans to establish a centralized, contemporary locust processing cottage. The government should educate processors on modern food processing and safety at all levels.
本研究调查了尼日利亚埃基蒂州购买现代刺槐豆加工设备的倾向。利用原始数据和多阶段采样策略,选择240个处理器进行研究。收集的数据采用描述性统计和推理统计工具,如Logit和普通最小二乘回归分析进行分析。大约72%的加工者已婚,平均有8年的加工刺槐豆科作物的经验。大多数受访者属于合作社(66.7%),无法获得信贷(100%)。加工的平均月收入为1.8万奈拉。大多数受访者(65%)不愿意为集中的、先进的或现代的处理设备的服务付费。Logit回归分析结果显示,显著影响集中式现代刺槐豆加工设备服务付费意愿的变量为家庭收入和加工经验(1%)、教育程度和年龄(5%)、家庭规模(10%)。普通最小二乘(OLS)分析显示,家庭规模和教育水平在1%水平上显著,年龄和婚姻状况在5%水平上显著,加工经验在1%水平上显著。该研究建议,准备好支付的加工商应该组建一个强大的合作社,以获得赠款或贷款,建立一个集中的、现代化的蝗虫加工小屋。政府应该在各个层面对加工者进行现代食品加工和安全教育。
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引用次数: 0
Minerals Composition of Solanum aethiopicum L. and Amaranthus hybridus L. Leaves from Burkina Faso 布基纳法索茄和苋菜叶的矿物组成
Pub Date : 2023-06-26 DOI: 10.9734/ejnfs/2023/v15i71320
Charles W. Yaméogo, F. Garanet
Aim: Malnutrition remains a public health problem in children aged under five years in Burkina Faso. Research to find indigenous vegetables with high content of minerals can contribute to fight against children malnutrition. The aim of this study was to assess minerals composition of Solanum aethiopicum L. and Amaranthus hybridus L. leaves grown in Burkina Faso. Methodes: The leaves have been collected in three markets of Ouagadougou. For the two plants, the dry leaves have been analysed for the following minerals content: Potassium (K), Calcium (Ca), Magnesium (Mg), Sodium (Na), Phosphor (P), Iron (Fe) and Zinc (Zn). The analyses have been done using the atomic absorption spectrophotometer and flame photometer methodes. Results: The results showed high mean concentration in K, Ca and Mg in the leaves of the two plants. For Solanum aethiopicum leaves, the concentration in K, Ca and Mg was respectively 3064; 1048 and 666 mg/100 g. The trace elements content were also high: Fe (12 mg/100 g) and Zn (20 mg/100 g). For Amaranthus hybridus leaves, the concentration in K, Ca and Mg was respectively 3573; 606 and 475 mg/100 g. The leaves of Solanum aethiopicum had the highest content of following minerals: Ca, Mg, Na, P, Fe and Zn compared to Amaranthus hybridus leaves. Conclusion: This study showed that both plants are good sources of important minerals. They are essential to be included in the diet of children to promote growth and contribute to fight against malnutrition.
目的:营养不良仍然是布基纳法索五岁以下儿童的一个公共卫生问题。研究发现富含矿物质的本土蔬菜有助于对抗儿童营养不良。本研究的目的是评估生长在布基纳法索的埃塞俄比亚茄和苋菜叶片的矿物质组成。方法:在瓦加杜古的三个市场采集。对这两种植物的干叶进行了以下矿物质含量分析:钾(K)、钙(Ca)、镁(Mg)、钠(Na)、磷(P)、铁(Fe)和锌(Zn)。用原子吸收分光光度计和火焰光度计进行了分析。结果:两种植物叶片中钾、钙、镁的平均浓度均较高。茄叶中K、Ca、Mg的浓度分别为3064;1048和666毫克/100克。微量元素含量也较高,Fe (12 mg/100 g)、Zn (20 mg/100 g),苋菜叶片中K、Ca、mg的浓度分别为3573;606和475毫克/100克。茄叶中Ca、Mg、Na、P、Fe和Zn的含量均高于苋菜叶。结论:本研究表明,这两种植物都是重要矿物质的良好来源。它们必须被纳入儿童的饮食中,以促进生长并有助于对抗营养不良。
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引用次数: 0
Molecular Detection of Enterotoxigenic Staphylococcus aureus in Ready-to-eat Cowhide (‘Ponmo’) Collected from Lagos Metropolis, Nigeria 尼日利亚拉各斯市即食牛皮(Ponmo)中产肠毒素金黄色葡萄球菌的分子检测
Pub Date : 2023-06-23 DOI: 10.9734/ejnfs/2023/v15i71319
Teniola, Olakunle David, Abisoye, Fiyinfoluwa Solomon, Folounso, Temitayo Veronica, Fowora Muinah, Ikuesan, Adeleke Felix, A. Tajudeen
Aims: Ready-to-eat [RTE] animal products like ponmo are preferred by consumers due to its palatability and quality. However, foodborne pathogens particularly Staphylococcus aureus are sources of concern due to cross-contamination of raw and cooked cowhide. This study aimed to investigate the incidence of enterotoxigenic S. aureus in ready-to-eat locally processed cowhide. Methodology: Sixty (60) RTE cowhide samples were collected from different locations in Lagos, Nigeria and analyzed using conventional microbiological and molecular techniques for the detection of toxigenic S. aureus contamination. Suspected S. aureus isolates were confirmed by the presence of thermostable endonuclease [nuc] gene in their genome. Results: Result showed that 25 (41.67%) and 20 (33.50%) samples harbored coagulase-positive S. aureus and 20 other bacterial species different from S. aureus, respectively while 15 (24.83%) of the tested ponmo samples yielded no bacterial growth. Thirteen of the 15 randomly selected from the 25 suspected isolates were confirmed as S. aureus by the presence of thermostable endonuclease [nuc] gene in their genome. Enterotoxigenic genes were confirmed in all the 13 PCR detected S. aureus. Enterotoxin B gene is most prevalent in ponmo. Multiplex PCR detection of S. aureus enterotoxins [SE] genes revealed the molecular detection of different isolates carrying staphylococcal enterotoxin types A and B, mixed strain carrying both staphylococcal enterotoxins type A and type D. Antibiotic susceptibility of 20 S. aureus isolates revealed varying degrees of susceptibility patterns against the antimicrobial agents. Generally, gentamicin 70% (14/20), azithromycin 75% (15/20), co-trimoxazole 85% (17/20), levofloxacin 95% (19/20) were the most effective antibiotics to S. aureus. A low, ≥50% susceptibility was recorded to chloramphenicol 55% (11/20) and nitrofurantoin 65% (13/20). A higher resistance to streptomycin (90%; 18/20) and ceftazidime (95%; 19/20) was identified, with resistance to ceftazidime being the highest (95%; 19/20). Conclusion: It can be concluded that RTE ponmo vended in the study sites is of low hygienic quality and may be of health risk to consumers. High level hygiene practice and good manufacturing practices are required during the production, distribution and marketing of ponmo to curb the potential health consequences of eating ponmo.
目的:即食[RTE]动物产品,如ponmo因其适口性和质量而受到消费者的青睐。然而,由于生牛皮和熟牛皮的交叉污染,食源性病原体,特别是金黄色葡萄球菌是令人担忧的来源。本研究旨在调查当地加工的即食牛皮中产肠毒素金黄色葡萄球菌的发生率。方法:从尼日利亚拉各斯不同地点采集60(60)份RTE牛皮样品,采用常规微生物学和分子技术对其进行分析,以检测产毒金黄色葡萄球菌污染。通过在金黄色葡萄球菌基因组中存在耐热内切酶[nuc]基因,证实了疑似金黄色葡萄球菌分离株的存在。结果:25份(41.67%)和20份(33.50%)样品检出凝固酶阳性金黄色葡萄球菌和20种与金黄色葡萄球菌不同的细菌,15份(24.83%)样品检出无细菌生长。从25株疑似分离株中随机选取的15株中,有13株被证实为金黄色葡萄球菌,其基因组中存在耐热性内切酶[nuc]基因。PCR检测到的13株金黄色葡萄球菌均存在产肠毒素基因。肠毒素B基因在ponmo中最为普遍。多重PCR检测金黄色葡萄球菌肠毒素[SE]基因,发现携带A型和B型葡萄球菌肠毒素的不同分离株,同时携带A型和d型葡萄球菌肠毒素的混合菌株,20株金黄色葡萄球菌对抗菌药物的药敏表现出不同程度的药敏模式。总的来说,庆大霉素70%(14/20)、阿奇霉素75%(15/20)、复方新诺明85%(17/20)、左氧氟沙星95%(19/20)是对金黄色葡萄球菌最有效的抗生素。对氯霉素的敏感性为55%(11/20),对呋喃妥因的敏感性为65%(13/20)。对链霉素有较高的耐药性(90%;18/20)和头孢他啶(95%;19/20),其中头孢他啶耐药性最高(95%;19/20)。结论:研究地点销售的RTE ponmo卫生质量较差,可能对消费者造成健康风险。在ponmo的生产、分销和销售过程中,需要高水平的卫生规范和良好的生产规范,以遏制食用ponmo对健康的潜在影响。
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引用次数: 0
Physicochemical and Nutritional Properties of Baby-Led Cookies Produced from Rice, Banana and Cashew-nut Flour Blends 由大米、香蕉和腰果粉混合制成的婴儿饼干的理化和营养特性
Pub Date : 2023-06-19 DOI: 10.9734/ejnfs/2023/v15i71318
Abraham Olasupo Oladebeye, Aderonke Adenike Oladebeye
Aim: Cookies from rice, banana and cashew-nut flour blends were prepared as alternative to gluten-free baby-led food for children. Place and Duration of Study: The study was carried out in the Chemistry Laboratory, Department of Science Laboratory Technology, University of Medical Sciences, Ondo, Nigeria and Food Processing Laboratory, Department of Food Technology, Auchi Polytechnic, Auchi, Nigeria between August, 2022 and January, 2023. Methodology: Baby-led weaning cookies were formulated from flour blends as 100% raw rice (RRC), 50% rice and 50% wheat (RWC) and 40% rice, 20% cashew and 40% unripe banana (RCB) and their physical properties, sensory attributes, proximate properties, mineral compositions, vitamin profiles and anti-nutrients were determined and compared with 100% commercial cookie (CMC) with the view to substituting wheat flour with suitable flour blends with enhanced nutritional quality. Results: The peak diameter (10.40±0.20 mm), thickness (6.37±0.06 mm) and weight (17.65±0.04 g) were obtained in RCB. Spread ratio was highest (2.66±0.11) and lowest (1.42±0.01) in CMC and RWC respectively while spread factor decreased from 100% in CMC to 77.19% (RRC), 53.46% (RWC) and 62.47% (RWC). The grittiness, texture, aroma, taste and general acceptability of RCB were significantly similar to those of CMC (P < 0.05). Na, K, Mg and Zn contents increased in RCB. Proximate compositions (%) of the cookies formulated varied significantly from CMC. Peak crude protein (14.49±0.59), crude fibre (4.03±0.02) and fat (32.22±0.00) in RCB, ash (3.28±0.02) and carbohydrate by difference (58.15±0.13) in CMC and moisture (18.19±0.11) in RRC. The peak values of fat-soluble vitamins (A, D, E, K) and water-soluble vitamins (B1, B2, B3, B6, B9, B12, C) were most abundant in RCB. The proportions (mg/g) of phytate, oxalate, tannins and phenols in the cookies formulated were comparatively lower than the lethal dose, implying that the cookies would be safe for consumption. Conclusion: RCB cookie had excellent nutritional quality, which, as a novel baby-led weaning cookie, could serve as a suitable alternative to commercial cookies.
目的:用大米、香蕉和腰果粉混合制成的饼干,作为儿童无麸质婴儿食品的替代品。研究地点和时间:该研究于2022年8月至2023年1月在尼日利亚奥奇医学大学科学实验室技术系化学实验室和尼日利亚奥奇理工学院食品技术系食品加工实验室进行。方法:采用100%生米(RRC)、50%米加50%小麦(RWC)、40%米、20%腰果和40%未熟香蕉(RCB)的混合面粉配制婴儿断奶饼干,测定其物理特性、感官特性、近似特性、矿物质成分、维生素和抗营养成分,并与100%商品饼干(CMC)进行比较,以期用合适的混合面粉替代小麦粉,提高营养品质。结果:获得RCB峰直径(10.40±0.20 mm)、厚度(6.37±0.06 mm)、重量(17.65±0.04 g)。CMC和RWC的扩散比最高(2.66±0.11),最低(1.42±0.01),而CMC的扩散系数从100%降至77.19% (RRC)、53.46% (RWC)和62.47% (RWC)。RCB的粒度、质地、香气、口感和一般接受度与CMC有显著性差异(P < 0.05)。RCB中Na、K、Mg、Zn含量增加。配制的饼干的近似成分(%)与CMC有显著差异。RCB中粗蛋白质(14.49±0.59)、粗纤维(4.03±0.02)、脂肪(32.22±0.00)、灰分(3.28±0.02)、碳水化合物(58.15±0.13)、水分(18.19±0.11)的差异值分别为CMC和RRC。脂溶性维生素(A、D、E、K)和水溶性维生素(B1、B2、B3、B6、B9、B12、C)在RCB中含量最高。该饼干中植酸盐、草酸盐、单宁酸和酚类物质的含量(mg/g)均低于致死剂量,可安全食用。结论:RCB饼干具有优良的营养品质,是一种新型的婴儿断奶饼干,可作为市场饼干的替代产品。
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引用次数: 0
Optimization Method of Whole Wheat Flour, Soybean Flour, Oyster Meat Powder for Nutrient- Dense Composite Flour 全麦粉、大豆粉、蚝油粉制备营养致密复合面粉的优化方法
Pub Date : 2023-06-14 DOI: 10.9734/ejnfs/2023/v15i71317
Wilson Tamunotonye Orunaboka, S. Kosoko, V. Wabali, A. Olokoshe
Aim: To study the production of Nutrient – Dense Composite Flour from the blends of whole wheat flour, soybean flour (full fat) and oyster meat powder. Study Design: The study was design using the D-optimal combination design of Response Surface Methodology (RSM). Place and Duration of Study: The study was carried out at Department of Food, Nutrition and Home Science, University of Port Harcourt (Processing of raw materials) and the Department of Food Technology, Federal Institute of industrial research Oshodi, Lagos (Analysis of raw materials) between October 2021 and August 2022. Methodology: The raw materials were each processed to have wholesome flours, and then they were combined according to the matrix generated, which had ranges of 70 – 100%, 0 – 22%, and 0 – 8% for whole wheat flour (WWF), soybean flour (SBF) and oyster meat powder (OMP) respectively. Results: The design was used to assess the significance (5% probability) of the moisture, fat, and protein content, which ranged from 8.09 to 11.37%, 1.80 to 8.52% and 9.70 to 19.07% respectively; the water absorption (72.00 - 79.10BU), dough stability (9.3 - 17.5BU) and mixing tolerance index (25 - 50BU); and lightness and yellowness, which ranged from 65.48 - 83.2 and 13.77 - 23.58 respectively, of the flour blends. Protein content, water absorption dough stability, and mixing tolerance index were prioritized while moisture content, fat content, and yellowness were minimized for the numerical optimization of the responses. This study highlights the possibilities of utilizing non-conventional raw materials in the production of composite flour with balance nutritional and baking qualities. Conclusion: The best flour combination was 72.51% whole wheat flour, 19.63% soybean flour, and 7.86% oyster meat powder.
目的:研究以全麦粉、大豆粉(全脂)和蚝油粉为原料生产营养致密复合面粉的工艺。研究设计:采用响应面法(RSM)的d -最优组合设计。研究地点和时间:该研究于2021年10月至2022年8月在哈科特港大学食品、营养和家庭科学系(原材料加工)和拉各斯奥肖迪联邦工业研究所食品技术系(原材料分析)进行。方法:将各原料加工成卫生粉后,根据生成的基质进行组合,其中全麦粉(WWF)、大豆粉(SBF)和蚝油粉(OMP)的组合范围分别为70 ~ 100%、0 ~ 22%和0 ~ 8%。结果:采用设计对水分、脂肪和蛋白质含量进行显著性评估(5%概率),其显著性范围分别为8.09 ~ 11.37%、1.80 ~ 8.52%和9.70 ~ 19.07%;吸水率(72.00 ~ 79.10BU)、面团稳定性(9.3 ~ 17.5BU)、耐混指数(25 ~ 50BU);混合面粉的明暗度为65.48 ~ 83.2,黄度为13.77 ~ 23.58。以蛋白质含量、吸水性、面团稳定性和混合耐受指数为优先指标,以水分含量、脂肪含量和黄度为最小值,对响应进行数值优化。本研究强调了利用非常规原料生产营养和烘焙品质平衡的复合面粉的可能性。结论:最佳面粉组合为72.51%全麦粉、19.63%大豆粉和7.86%蚝油粉。
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引用次数: 0
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European Journal of Nutrition &amp; Food Safety
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