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Osmoregulatory Appraisal of Some Osmoprotectants on Hydrolytic Activities of Some Enzymes on Seeds of Two Water Stressed Cultivars of Sorghum bicolor (Moench) 几种渗透保护剂对两个缺水高粱品种种子部分酶水解活性的渗透调节评价
Pub Date : 2023-03-09 DOI: 10.9734/ejnfs/2023/v15i21294
Ojewumi, Anthony Wale
Enzymes play significant roles in metabolic processes of seeds. Therefore, this study evaluated osmoregulatory potential of some osmoprotectants on activities of some hydrolytic enzymes in the seeds of two cultivars (SOSAT.C-88 and CV. LCIC 9702) of sorghum bicolor. Matured seeds of the two cultivars were harvested and prepared for alpha, beta, total amylase and proteinase activities assay. The osmoprotectants produced significant variations on the enzymes at 10 and 14 days (DA) of 8 weeks after treatments (WAT). Seeds of well-watered SOSAT.C-88 produced higher alpha (2.10 IU/ml), beta (1.70 IU/ml) and total amylase activities (3.30 IU/ml) at 14 days (DA). Higher alpha (2.01 IU/ml and total amylase activities (2.61 IU/ml) were recorded in the seeds of CV. LCIC 9702 well-watered at 14 days DA 8WAT. Furthermore, total amylase activities (3.87 IU/ml) were recorded in the seeds produced by CV. LCIC 9702 well-watered at 14 days DA. Significant increase was noticed in beta (1.14 IU/ml) and alpha amylase (1.58 IU/ml) in the seeds of CV. LCIC 9702 treated with mycorrhiza. CV. LCIC 9702 well watered produced highest proteinase activities (1.57 U/ml) while least of the parameters were recorded in SOSAT.C-88 and CV. LCIC 9702 droughted. In conclusion, the osmoprotectants had regulatory effects on the activities of hydrolytic enzymes therefore the use of the osmoprotectants in farming should be encouraged.
酶在种子代谢过程中起着重要作用。因此,本研究评估了几种渗透保护剂对SOSAT.C-88和CV两个品种种子中某些水解酶活性的渗透调节潜力。LCIC 9702)高粱双色。收获两个品种的成熟种子,进行α、β、总淀粉酶和蛋白酶活性测定。渗透保护剂在处理后8周(WAT)的第10天和第14天(DA)对酶产生显著变化。水分充足的SOSAT.C-88种子在第14天(DA)产生较高的α (2.10 IU/ml)、β (1.70 IU/ml)和总淀粉酶活性(3.30 IU/ml)。CV种子α和总淀粉酶活性分别为2.01 IU/ml和2.61 IU/ml。LCIC 9702在DA 8WAT 14天水充足。此外,CV生产的种子中总淀粉酶活性为3.87 IU/ml。LCIC 9702在DA 14天浇水充足。CV种子中β -淀粉酶(1.14 IU/ml)和α -淀粉酶(1.58 IU/ml)显著升高。LCIC 9702用菌根处理。简历。水分充足的LCIC 9702蛋白酶活性最高(1.57 U/ml),而SOSAT.C-88和CV的蛋白酶活性最低。LCIC 9702干旱。综上所述,渗透保护剂对水解酶活性具有调节作用,应在农业生产中推广应用。
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引用次数: 0
Physicochemical Properties, Heavy Metals and Aflatoxins Content of Crude Palm and Groundnut Oils Produced and Marketed in Benue State, Nigeria 尼日利亚贝努埃州生产和销售的棕榈油和花生油的理化性质、重金属和黄曲霉毒素含量
Pub Date : 2023-03-04 DOI: 10.9734/ejnfs/2023/v15i21293
Q. M. Amua, J. Abah, G. M. Muyong, N. B. Bongjo
The study aims at assessing the physicochemical properties, heavy metals, and aflatoxins content of crude palm and groundnut oils produced and sold in the Adikpo, Wadata, and Otukpa areas of Benue state, Nigeria. Specific gravity, refractive index, acid value, saponification value, peroxide value, moisture content, and smoke point are the physicochemical properties evaluated. The heavy metals analyzed include; Lead, Nickel, Copper, Iron, Arsenic, and Iron. Total aflatoxins were determined by Enzyme-linked immunosorbent assay (ELISA).  Findings revealed that most of the physicochemical properties of palm and groundnut oils from these areas deviated from the Food and Agricultural Organization/World Health Organization (FAO/WHO) standard range. A high amount of Cadmium (0.89 mg/kg, 1.01 mg/kg, and 0.92 mg/kg) was detected in the local groundnut oil samples but lesser in palm oils from Adikpo, Wadata, and Otukpa respectively, while Copper content was within the FAO/WHO safe limits. The arsenic content of palm oil produced in the region was not as high as those in groundnut oil but was higher than the recommended maximum limit of 0.1 mg/kg. The Nickel content in the oil samples was higher than the 0.50 mg/kg permissible limit, but Lead was within the safe limits. Total Aflatoxin content in crude groundnut oil was within the range of 9.05 ppm to 10.13 ppm, while a range of 2.03 ppm to 2.74 ppm was recorded in crude palm oil. The locally produced oils are of lower quality, suggesting that refining should be adopted and quality seeds should be used for the extraction of oils.
该研究旨在评估尼日利亚贝努埃州Adikpo、Wadata和Otukpa地区生产和销售的粗棕榈油和花生油的理化性质、重金属和黄曲霉毒素含量。比重、折射率、酸值、皂化值、过氧化值、水分含量和烟点是评估的物理化学性质。分析的重金属包括;铅,镍,铜,铁,砷和铁。采用酶联免疫吸附法(ELISA)测定黄曲霉毒素总量。调查结果显示,来自这些地区的棕榈油和花生油的大部分理化性质偏离了粮食及农业组织/世界卫生组织(FAO/WHO)的标准范围。在当地花生油样品中检测到大量镉(0.89 mg/kg、1.01 mg/kg和0.92 mg/kg),但在Adikpo、Wadata和Otukpa的棕榈油样品中检测到的镉含量较低,而铜含量在粮农组织/世界卫生组织的安全限值之内。该地区生产的棕榈油的砷含量不像花生油那样高,但高于建议的最高限量0.1毫克/公斤。油样中镍含量高于0.50 mg/kg允许限量,铅含量在安全范围内。花生粗油中黄曲霉毒素总含量在9.05 ~ 10.13 ppm之间,棕榈油粗油中黄曲霉毒素总含量在2.03 ~ 2.74 ppm之间。当地出产的油脂质量较差,建议采用精制工艺,并选用优质种子进行油脂提取。
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引用次数: 0
Proposal for Educational Intervention in People Living with Type 2 Diabetes Mellitus and in their Family Caregiver 2型糖尿病患者及其家庭照护者的教育干预建议
Pub Date : 2023-02-21 DOI: 10.9734/ejnfs/2023/v15i21291
Aldo Pelcastre Neri, J. Vázquez, L. Lydia, Inés Tenahua Quitl, Olga Rocío Flores Chávez, María del Carmen López Zermeño, Jesús Carlos Ruvacaba Ledezma
Objective: Design a proposal of educational intervention for both, people who live with Diabetes Mellitus type 2 and their family caregiver. Methodology: Methodology. A case series study was conducted with the participation of 12 people living with type 2 diabetes mellitus and attending a Mutual Help Group in Pachuquilla, Hidalgo. Sociodemographic data, somatometric measurements (weight, height, BMI, waist circumference), blood pressure, and capillary blood samples for glycosylated hemoglobin were collected. Results: The mean age was 64 and range of 39-79 years, O.D. of 12; 67% women; 25% finished secondary school, 50% worked at home, 17% in commerce, 33% unemployed; 58% married, 25% widowed, and 17% divorced and in common law; 33.% with less than 5 years diagnosed, 67% have been diagnosed for more than 5 years; 75% depend economically; 67% have complications, 75% with family support, in diabetes knowledge 33% obtained intermediate score and 67% inadequate score; the weight average was of 77 kg and range of 57-116.5 kg, and O.D. 17 kg; in waist circumference, 100% exceed the recommended limit, in BMI, 50% is classified as overweight and 50% as obese; in blood pressure 50% > 130/80 mmHg and in glycosylated hemoglobin 75% > 7% mg/dl. Conclusion: The currently implemented strategy does not reflect good knowledge and control of the disease. This study invites us to continue with research where an educational intervention is implemented in which family caregivers are included and active participation is encouraged, in addition to implementing the IEP as a theoretical basis for the intervention.
目的:设计针对2型糖尿病患者及其家庭照顾者的教育干预方案。方法:方法。在伊达尔戈州帕丘基利亚的一个互助小组中,对12名2型糖尿病患者进行了一项病例系列研究。收集社会人口统计数据、体测数据(体重、身高、BMI、腰围)、血压和毛细血糖化血红蛋白样本。结果:患者平均年龄64岁,年龄范围39 ~ 79岁,od值12;67%的女性;25%中学毕业,50%在家工作,17%经商,33%失业;58%已婚,25%丧偶,17%离婚或同居;33.确诊时间少于5年的占%,确诊时间超过5年的占67%;75%的人在经济上依赖;67%有并发症,75%有家庭支持,在糖尿病知识方面,33%获得中等评分,67%得分不足;平均体重为77 kg,范围为57 ~ 116.5 kg, od值为17 kg;腰围100%超过建议限制,BMI 50%为超重,50%为肥胖;血压50% > 130/80 mmHg,糖化血红蛋白75% > 7% mg/dl。结论:目前实施的策略不能反映对疾病的良好认识和控制。本研究邀请我们继续研究实施教育干预,其中包括家庭照顾者并鼓励积极参与,除了实施IEP作为干预的理论基础之外。
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引用次数: 0
Antibacterial Activities of Extracts from four Wild Food Fruits 四种野生食用水果提取物的抗菌活性研究
Pub Date : 2023-01-31 DOI: 10.9734/ejnfs/2023/v15i11289
M. Konaré, Singou Keita, M. Somda, Issiaka Togola, N. Diarra, R. Sanogo
Aims: This study aimed to assess the in vitro antibacterial effects of extracts from four wild food fruits: Balanites aegyptiaca, Saba senegalensis, Ziziphus mauritiana, and Raphia sudanica. Place and Duration of Study: The samples of plant material were collected at Banamba and Sikasso, Mali between January and May 2018. The bacterial strains were collected at Research Centre for Biological Food and Nutritional Sciences (CRSBAN), University Professor Joseph Ki-Zerbo; Ouagadougou, Burkina Faso. The experimental parts were also carried out at CRSBAN from October 2019 to January 2020. Methodology: The fruit extracts were screened for antibacterial activity against Escherichia coli, Salmonella typhi, S. aureus, B. cereus, and L. monocytogenes strains. The diameters of the inhibition zones (ID), the minimum inhibitory concentrations (MIC) as well as the minimum bactericidal concentrations (MBC) were evaluated using agar diffusion method. Results: The findings revealed that these parameters have varied as a function of fruit species and/or their zones of provenances. All fruit extracts showed significant growth reducing effect against all the tested bacteria. The extracts from R. sudanica have exhibited the strongest growth-inhibiting activity specifically against E. coli (ID = 15.33±0.58 mm) and Salmonella typhi (ID = 18.00±1.00 mm) with lower MIC (from 2.08±1.44 to 5.83±1.44 mg/mL). Moreover, the MBC/MIC ratios revealed that the extracts from the studied fruits possess mainly bacteriostatic effects towards the tested strains. Conclusion: These findings support local therapeutics properties attributed to these fruits. They also demonstrate that, in addition to their nutritional values, these edible fruits could be used for developing antibiotics to treat infectious diseases and food poisoning.
目的:研究埃及巴兰、塞内加尔巴兰、毛里求斯巴兰和苏丹拉菲四种野生食用水果提取物的体外抑菌作用。研究地点和时间:植物材料样本于2018年1月至5月在马里的Banamba和Sikasso收集。细菌菌株收集于生物食品与营养科学研究中心(CRSBAN),大学教授Joseph Ki-Zerbo;瓦加杜古,布基纳法索。实验部分也于2019年10月至2020年1月在CRSBAN进行。方法:筛选水果提取物对大肠杆菌、伤寒沙门氏菌、金黄色葡萄球菌、蜡样芽孢杆菌和单核增生乳杆菌的抑菌活性。采用琼脂扩散法测定抑菌带直径(ID)、最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果:研究结果表明,这些参数随果实种类和/或其种源区域的变化而变化。所有水果提取物对所有被试细菌均有显著的抑菌作用。丹参提取物对大肠杆菌(ID = 15.33±0.58 mm)和伤寒沙门氏菌(ID = 18.00±1.00 mm)的生长抑制活性最强,MIC(2.08±1.44 ~ 5.83±1.44 mg/mL)较低。此外,MBC/MIC比值表明,所研究果实提取物对被试菌株具有主要的抑菌作用。结论:这些发现支持这些水果的局部治疗特性。他们还证明,除了营养价值外,这些可食用水果还可以用于开发治疗传染病和食物中毒的抗生素。
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引用次数: 0
Chemical and Biological Evaluation of Bakeries Produced from Golden Berries 金莓烘焙食品的化学和生物学评价
Pub Date : 2023-01-31 DOI: 10.9734/ejnfs/2023/v15i21290
G. El-Hadidy, Shereen, L. Nassef, Elshahat, G. El-Dreny
This investigation was performed with the purpose of researching the influence of  pizza containing dried golden berry fruits (DGBF) at different doses against carbon tetrachloride - induced hepatotoxicity in rats. The study shows phenols content of golden berry. 25 male rats were used in the biological investigation. Rats were divided into five groups (5 rats in group) the investigation was 12 weeks. The first group (negative group) was given a basal diet and the second group (G2, G3, G4, and G5) was injected intramuscularly with carbon tetrachloride 2 ml/kg BW (50% v/v in liquid paraffin) weekly to induce hepatotoxicity. After the injury, group G3, G4 and G5 fed on 50% basal diet supplemented with 50%pizza containing 5, 10 and 15% DGBF. Findings indicate that DGBF had a high antioxidant activity, total phenol, flavonoids, ascorbic acid and carotenoids content. Rats fed 50% pizza containing (5,10 and 15%) DGBF had a lower serum total cholesterol, LDL cholesterol, triglyceride, urea, uric acid, creatinine, GOT, GPT, MDA and SOD compared to rats fed simply the basal diet (positive control). The DGBF was added to the pizza with different proportions, and its sensory properties were evaluated, and all proportions were proper to the panelists, compared to the control. The findings of this work suggest that golden berries could be used to treat and prevent hepatotoxicity patients.
本实验旨在研究不同剂量金浆果干披萨对大鼠四氯化碳肝毒性的影响。研究表明金莓中酚类物质的含量。采用25只雄性大鼠进行生物学调查。将大鼠分为5组,每组5只,为期12周。第一组(阴性组)给予基础日粮,第二组(G2、G3、G4、G5)每周肌肉注射2 ml/kg BW(液体石蜡50% v/v)的四氯化碳诱导肝毒性。损伤后,G3、G4和G5组分别饲喂50%基础饲粮和50%含5%、10%和15% DGBF的披萨。结果表明,DGBF具有较高的抗氧化活性,总酚、类黄酮、抗坏血酸和类胡萝卜素含量较高。与仅饲喂基础日粮(阳性对照)的大鼠相比,喂食含有(5、10和15%)DGBF的比萨的大鼠血清总胆固醇、低密度脂蛋白胆固醇、甘油三酯、尿素、尿酸、肌酐、GOT、GPT、MDA和SOD均较低。将DGBF以不同比例添加到比萨中,并对其感官性能进行评估,与对照组相比,所有比例对小组成员都是合适的。本研究结果提示,金莓可用于治疗和预防肝毒性患者。
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引用次数: 0
The Lack of Nutritional Competency among the Medical Practitioners and Medical Students: A Systematic Review 医生和医学生营养能力的缺乏:一项系统综述
Pub Date : 2023-01-27 DOI: 10.9734/ejnfs/2023/v15i11287
M. James
Objective: This systematic review addressed the effects of the competency level of medical students, physicians, and practitioners in nutrition education for optimum patient care. It also addresses the perceived inadequate didactic contact hours of nutrition education. Method: There were 55 studies selected, and 25 were used for this review including quantitative and qualitative studies. The data were divided into four groups: quantitative data, qualitative data, reports, and news articles.  The categorization of the literature was as follows: 17 quantitative, 2 qualitative, 4 news articles, 1 manual, and 1 symposium report. These were evaluated to produce a credible qualitative meta-analysis of available data. Data Sources: The systematic review used databases and citation indexes including Embosses, PubMed, JAMA Network, Medline, Elsevier, and Oxford Academics, these include journals, reports of Symposiums, and news articles to ascertain evidence-based data Results: The findings of this review revealed the significant effect inadequate contact nutrition education has on the competency level of medical students, physicians, and practitioners. Limitations: Limitations of this review include several external factors. Although universities are expected to implement approximately 44 hours of nutrition education for the competency level of medical students and physicians during their tenure, this may not be the case at all schools.  Many offer only 15-25 didactics hours. In addition, in some cases, the comparisons are not equal but the researcher was unable to establish the hours and a basic curriculum structure.
目的:本系统综述探讨了医学生、医生和从业人员在营养教育中的能力水平对最佳患者护理的影响。它还解决了人们认为营养教育教学接触时间不足的问题。方法:共纳入55篇文献,其中定量研究和定性研究共25篇。数据分为四组:定量数据、定性数据、报告和新闻文章。文献分类:定量文献17篇,定性文献2篇,新闻文献4篇,手册文献1篇,会议报告文献1篇。我们对这些数据进行了评估,对现有数据进行了可靠的定性荟萃分析。数据来源:系统评价使用了数据库和引文索引,包括Embosses、PubMed、JAMA Network、Medline、Elsevier和Oxford Academics,其中包括期刊、专题讨论会报告和新闻文章,以确定循证数据。结果:本评价的发现揭示了接触营养教育不足对医学生、医生和从业人员的能力水平有显著影响。局限性:本综述的局限性包括几个外部因素。虽然预计大学将在医学生和医生的任期内实施大约44小时的营养教育,以提高他们的能力水平,但并非所有学校都是如此。许多学校只提供15-25小时的教学时间。此外,在某些情况下,比较是不平等的,但研究人员无法建立学时和基本的课程结构。
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引用次数: 0
Effect of Integrated Nutrient Management on Growth and Yield of Cauliflower 综合营养管理对花椰菜生长和产量的影响
Pub Date : 2023-01-10 DOI: 10.9734/ejnfs/2023/v15i11286
Samsun Nahar Hashi, T. Mostarin, K. Khatun, Sayma Kabir, Shapla Akter, Khodaiza Banu, Sanchita Roy, A. Ahmed, Md. Abdus Samad
With the use of integrated nutrient management, cauliflower the growth and yield can be boosted. Due to their complementing effects, the ideal combination of different organic and inorganic sources of nutrients may significantly boost cauliflower growth and yield. The experiment consisted of 13 treatments viz. T1= N120P60K100S20 kg/ha (Recommended dose of NPKS as control), T2= N120P60K100S20 kg/ha + CD (5 t/ha), T3= N120P60K100S20 kg/ha + VC (4 t/ha), T4= N120P60K100S20 kg/ha + MSC (4 t/ha), T5= N120P60K100S20B0.6Mo0.54 kg/ha + CD (5 t/ha), T6= N120P60K100S20B0.6Mo0.54 kg/ha + VC (4 t/ha), T7= N120P60K100S20B0.6Mo0.54 kg/ha + MSC (4 t/ha), T8= N120P60K100S20 kg/ha + CD (5 t/ha) + Bio. (5 kg/ha), T9= N120P60K100S20 kg/ha + VC (4 t/ha) + Bio. (5 kg/ha), T10= N120P60K100S20 kg/ha + MSC (4 t/ha) + Bio. (5 kg/ha), T11= N120P60K100S20B0.6Mo0.54 kg/ha + CD (5 t/ha) + Bio. (5 kg/ha), T12= N120P60K100S20B0.6Mo0.54 kg/ha + VC (4 t/ha) + Bio. (5 kg/ha) and T13= N120P60K100S20B0.6Mo0.54 kg/ha + MSC (4 t/ha) + Bio. (5 kg/ha). The experiment was laid out in a Randomized Complete Block Design (RCBD) having single factor with three replications. Data were recorded on growth, yield components of cauliflower and significant variation was observed for most of the studied characters. Under this investigation, it was revealed that the highest yield (36.34 t/ha) with net return (Tk. 524202) and BCR (3.59) was obtained from T12 (N120P60K100S20B0.6Mo0.54 kg/ha + VC (4 t/ha) + Bio. (5 kg/ha) treatment. On the other hand, the lowest yield (13.50 t/ha) with net return (Tk. 137869) and BCR (2.04) was obtained from T1 (N120P60K100S20 kg/ha) treatment. So, economic analysis revealed that T12 (N120P60K100S20B0.6Mo0.54 kg/ha + VC (4 t/ha) + Bio-fertilizer (5 kg/ha) treatment appeared to be the best for achieving the higher growth, yield and economic benefit of cauliflower.  
采用综合营养管理,可以促进花椰菜的生长和产量。由于有机和无机营养源的互补作用,不同营养源的理想组合可以显著促进花椰菜的生长和产量。实验由13个治疗即T1 = N120P60K100S20公斤/公顷(氮磷钾的推荐剂量控制),T2 = N120P60K100S20公斤/公顷+ CD(5吨/公顷),T3 = N120P60K100S20公斤/公顷+ VC(4吨/公顷),T4 = N120P60K100S20公斤/公顷+ MSC(4吨/公顷),T5 = N120P60K100S20B0.6Mo0.54公斤/公顷+ CD(5吨/公顷),T6 = N120P60K100S20B0.6Mo0.54公斤/公顷+ VC(4吨/公顷),T7 = N120P60K100S20B0.6Mo0.54公斤/公顷+ MSC(4吨/公顷),T8 = N120P60K100S20公斤/公顷+ CD(5吨/公顷)+生物。(5公斤/公顷),T9= N120P60K100S20公斤/公顷+ VC(4吨/公顷)+ Bio。(5公斤/公顷),T10= N120P60K100S20公斤/公顷+ MSC(4吨/公顷)+ Bio。(5 kg/ha), T11= N120P60K100S20B0.6Mo0.54 kg/ha + CD (5 t/ha) + Bio。(5 kg/ha), T12= N120P60K100S20B0.6Mo0.54 kg/ha + VC (4 t/ha) + Bio。(5 kg/ha)和T13= N120P60K100S20B0.6Mo0.54 kg/ha + MSC (4 t/ha) + Bio。(5公斤/公顷)。实验采用随机完全区组设计(RCBD),单因素,3个重复。对花椰菜的生长、产量组成进行了记录,大部分研究性状均有显著变化。结果表明,T12 (N120P60K100S20B0.6Mo0.54 kg/ha + VC (4 t/ha) + Bio)产量最高(36.34 t/ha),净收益(Tk. 524202), BCR(3.59)。(5公斤/公顷)处理。另一方面,T1 (N120P60K100S20 kg/ha)处理的产量最低(13.50 t/ha),净收益(Tk. 137869)和BCR(2.04)。因此,经济分析表明,T12 (N120P60K100S20B0.6Mo0.54 kg/ha) + VC (4 t/ha) +生物肥料(5 kg/ha)处理对花椰菜的生长、产量和经济效益均有较好的促进作用。
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引用次数: 0
Effect of Thermal Treatments on Selected Minerals and Water Soluble Vitamins of Chicken Breast Meat 热处理对鸡胸肉中部分矿物质和水溶性维生素的影响
Pub Date : 2023-01-05 DOI: 10.9734/ejnfs/2023/v15i11285
S. U. Alugwu, T. Okonkwo, M. Ngadi
The study was conducted to ascertain the effect of thermal treatments on selected minerals (Ca, K, Mg, Na, P, Fe and Zn) and water soluble vitamins (B1, B2, B3, B6, B9, B12 and C) contents of chicken breast meat. Industrial skinless chicken breast meat samples were purchased, transported to Bioprocess laboratory in cool conditions, frozen and sliced into dimensions and thawed. The samples were cooked by air frying (AF), baking (BK), deep fat frying (DF) and grilling (GR) at 170, 180 and 1900C for 0, 4, 8 and 12 min for minerals and 0, 8 and 16 min for vitamins. Thereafter, cooked and raw samples were wet acid digested overnight and 5 h digested on a block digester on slowly increased temperature to 1200C, cooled and deionized. The mineral elements were analysed by Optima 4300DV inductivity coupled plasma optical emission spectrometry (ICP-OES) and inductivity coupled plasma mass spectrometry (ICP-MS). These mineral elements were extrapolated through a calibration curve between intensity and concentration, while the vitamins were ascertained by measurement of absorbance of filtrates of the samples dissolved in their respective solvents in the Spectrophotometer against their blank samples at different wavelengths. The results showed that cooking methods decreased significantly (p < 0.05) the mineral elements with an exception of Zn cooked by grilling (GR) that increased by 19.92% and Mg that increased in the cooking methods. The ascending percentage reduction of minerals in cooked chicken breast were Zn, P, K, Fe, Na and Ca. Samples cooked by DF had significantly (p < 0.05) higher percentage reduction of 45.06% in Ca, 27.74% in Na and 18.85% in Zn and higher percentage increases of 14.96% in Mg contents than other methods. Also samples cooked by DF had higher percentage reductions of 55.10%, 37.93%, 37.11%, 34.44% and 30.99% in vitamins B1, C, B2, B9 and B6  Whereas higher percentage reductions of 41.67% and 37.84 % in vitamins B12 and B3 occurred in baking (Bk) and grilling (GR) treated samples. Cooking at 1900C had higher percent reduction in the Ca, Na, Fe, K, P and Zn as well as B1, B12, B2, C, B3, B9 and B6. Cooking methods, temperatures and times decreased significantly (p < 0.05) vitamins and minerals contents of chicken breast meat with an exception of Mg. Samples cooked at 1700C for 4 min and 1700C for 8 min had lower losses of minerals and vitamins compared to similar samples cooked at 1800C and 1900C. The AF cooking method had the least percent reduction of 22.50% than other cooking methods BK (26.88%), DF (36.04%) and GR (30.69%) in vitamin contents.
本试验旨在研究热处理对鸡胸肉中选定矿物质(Ca、K、Mg、Na、P、Fe和Zn)和水溶性维生素(B1、B2、B3、B6、B9、B12和C)含量的影响。购买工业去皮鸡胸肉样品,在凉爽的条件下运送到生物工艺实验室,冷冻,切片,解冻。样品分别在170、180和1900℃的温度下进行空气煎炸(AF)、烘烤(BK)、油炸(DF)和烧烤(GR),分别为0、4、8和12 min的矿物质和0、8和16 min的维生素。然后,将煮熟的和生的样品湿酸消化过夜,并在块状消化器上缓慢升高温度至1200℃消化5 h,冷却并去离子。采用Optima 4300DV电感耦合等离子体发射光谱法(ICP-OES)和电感耦合等离子体质谱法(ICP-MS)对矿物元素进行分析。这些矿物元素是通过强度和浓度之间的校准曲线来推断的,而维生素是通过分光光度计中溶解在各自溶剂中的样品的滤液对其空白样品在不同波长下的吸光度来确定的。结果表明:不同蒸煮方式均显著降低了矿质元素含量(p < 0.05),但不同蒸煮方式中锌含量升高了19.92%,Mg含量升高了。鸡胸肉中锌、磷、钾、铁、钠和钙的还原比例依次上升,其中DF法对Ca、Na和Zn的还原比例分别为45.06%、27.74%和18.85% (P < 0.05),对Mg的还原比例为14.96% (P < 0.05)。经DF处理的样品中维生素B1、C、B2、B9和B6的含量分别减少了55.10%、37.93%、37.11%、34.44%和30.99%,而经过烘烤(Bk)和烧烤(GR)处理的样品中维生素B12和B3的含量分别减少了41.67%和37.84%。在1900℃下烹饪,钙、钠、铁、钾、磷、锌以及B1、B12、B2、C、B3、B9和B6的减少率更高。除Mg外,不同烹饪方式、温度和时间显著降低了鸡胸肉中维生素和矿物质的含量(p < 0.05)。在1700C下煮4分钟和1700C下煮8分钟的样品与在1800C和1900C下煮的类似样品相比,矿物质和维生素的损失更低。与BK(26.88%)、DF(36.04%)和GR(30.69%) 3种蒸煮方法相比,AF蒸煮方法的维生素含量降低幅度最小,分别为22.50%。
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引用次数: 1
Microbial Load, Antibiotic Resistant Bacteria and Antibiotic Residues in Broiler Chicken 肉鸡微生物负荷、耐药菌和抗生素残留
Pub Date : 2023-01-02 DOI: 10.9734/ejnfs/2023/v15i11284
V. Kola, K. Srikanth, Shaik Muzammil Pasha, Y. S. Goutham, C. Pasha
Chicken meat is highly preferred protein food worldwide. To meet the demand, huge poultry farms are established and using antibiotics as prophylaxis and treatment against the bacterial diseases. Uncontrolled usage of antibiotics has led to development of antibiotic resistance in poultry and antibiotic residues in poultry chicken. Fifty one chicken meat samples were collected from various retail outlets. Antibiotic residues were quantified by HPLC, total microbial load was measured by growth of bacteria on growth medium and antibiotic resistant profile of Escherichia coli, Salmonella spp, Staphylococcus aureus and Campylobacter spp was determined by well diffusion method. Except neomycin, all tested antibiotics were present in the range of 10-978 ppm, the average microbial load was in the range log 10 of 7.32 per gram of chicken sample, E. coli, Salmonella spp, Staphylococcus aureus and Campylobacter spp were resistant to several antibiotics studied. Hence there is a need of appropriate usage of antibiotics in poultry and proper handling of chicken during farming and slaughtering.
鸡肉是全世界最受欢迎的蛋白质食品。为了满足需求,建立了大型家禽养殖场,并使用抗生素预防和治疗细菌性疾病。不加控制地使用抗生素已导致家禽产生抗生素耐药性和家禽鸡产生抗生素残留。在多个零售点采集了51个鸡肉样本。采用高效液相色谱法测定抗生素残留量,利用细菌在培养基上的生长测定微生物总负荷,采用孔扩散法测定大肠杆菌、沙门氏菌、金黄色葡萄球菌和弯曲杆菌的耐药谱。除新霉素外,所有抗生素均在10-978 ppm范围内,平均微生物负荷在每克鸡样品7.32的log10范围内,大肠杆菌、沙门氏菌、金黄色葡萄球菌和弯曲杆菌对几种抗生素均有耐药性。因此,需要在家禽中适当使用抗生素,并在饲养和屠宰期间妥善处理鸡只。
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引用次数: 0
Proximate Composition and Microbiological Quality of “Kunu-Aya”: A Locally Produced Non-Fermented Beverage in Nigeria “Kunu-Aya”的近似组成和微生物质量:尼日利亚当地生产的非发酵饮料
Pub Date : 2022-12-30 DOI: 10.9734/ejnfs/2022/v14i121292
F. Akubuenyi, O. H. Sylvanus
Kunu-aya” is non- fermented beverage locally produced from Cyperus esculentus (Tiger nut) and Phoenix dactylifera (Dates) based milk. This study determined the proximate composition and microbial quality of laboratory produced “Kunu-aya” following standard methods. Biochemical characterization method was carried out for identification of associated isolates.  The results revealed that the “Kunu-aya” contains; moisture content was 59.44±0.05, Ash content of 5.02±0.07, crude fat 8.108±0.03, Crude fibre 9.16±0.04, crude protein 8.983±0.26, and carbohydrate 9.3±0.05 and the energy content was 182.69kcal. “Kunu-aya” also contains significant amounts of magnesium (Mg) 2.045±0.05, potassium (K) 40.506±0.05, phosphorus (P) 4.506±0.03, copper (Cu) 0.018±0.04, zinc (Zn) 48.001±0.01, iron (Fe) 0.408±0.04, sodium (Na) of 6.031±0.07 and calcium (Ca) 0.196±0.03. The total viable bacteria count of the sample was 4.0 x 104 CFU/ml and the total fungal count was 2.7x 102 CFU/ml. Species of  Staphylococcus spp (50%), Bacilli spp. (16.7%) Streptococcus spp. (16.7%) and Micrococcus spp. (16.7%) are predominant bacteria contaminants of the beverage. Besides, fungi species of Aspergillus spp. (60%), Penicillium spp. (20%) and Rhizopus spp. (20%) were also isolated. The microbial load obtained exceeded the acceptable limit stipulated for dairy drinks. Based on the proximate and mineral contents of “Kunu-aya”, its rich essential contents can provide nutrients for human nutrition. Therefore, the production of this beverage under strict hygiene conditions will improve the microbiological quality and reduce the health risk associated with its consumption.
“Kunu-aya”是一种不发酵的饮料,由当地的虎坚果和凤凰花(枣子)牛奶制成。本研究采用标准方法测定了实验室生产的“Kunu-aya”的近似成分和微生物质量。采用生化鉴定方法对相关菌株进行鉴定。结果表明,“Kunu-aya”含有;水分含量为59.44±0.05,灰分含量为5.02±0.07,粗脂肪含量为8.108±0.03,粗纤维含量为9.16±0.04,粗蛋白质含量为8.983±0.26,碳水化合物含量为9.3±0.05,能量含量为182.69kcal。“Kunu-aya”还含有大量的镁(Mg) 2.045±0.05,钾(K) 40.506±0.05,磷(P) 4.506±0.03,铜(Cu) 0.018±0.04,锌(Zn) 48.001±0.01,铁(Fe) 0.408±0.04,钠(Na) 6.031±0.07和钙(Ca) 0.196±0.03。样品活菌总数为4.0 × 104 CFU/ml,真菌总数为2.7 × 102 CFU/ml。葡萄球菌(50%)、芽孢杆菌(16.7%)、链球菌(16.7%)和微球菌(16.7%)是饮料中主要的细菌污染物。此外,还分离到曲霉(60%)、青霉(20%)和根霉(20%)等真菌。所获得的微生物负荷超过了乳制品规定的可接受限度。从“昆努亚”的近矿物质含量来看,其丰富的必需成分可以为人体提供营养。因此,在严格的卫生条件下生产这种饮料,可以提高微生物质量,降低与消费有关的健康风险。
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引用次数: 0
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European Journal of Nutrition &amp; Food Safety
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