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Chemical composition of summer truffle (Tuber aestivum Vittad.) from Bosnia and Herzegovina 波斯尼亚和黑塞哥维那夏季松露(Tuber aestivum Vittad.)的化学成分
Pub Date : 2023-11-30 DOI: 10.21303/2504-5695.2023.003191
S. Ljubojević, L. Vasilišin, G. Vučić, Ana Velemir
Ascocarps of summer truffle (Tuber aestivum Vittad.) were found in natural oak and beech forests on calcareous soils, at altitude of 840–850 m, near the town of Šipovo in Bosnia and Herzegovina (B&H), with the help of trained dogs.  Until now, no one in B&H has researched the chemical composition of summer truffle. The aim of the work is to analyze nutritional value, elemental composition, and the assortment of fatty acids in the freshly collected ascocarps and to compare achieved results with the results from the literature related to the summer truffle ascocarps found in some other locations, then with the ascomata of other species of truffles, and with the fruiting bodies of some above-ground edible mushrooms. Chemical analysis showed that Tuber aestivum contains about 75.5 % water and about 25.5 % dry matter. The most common group of compounds were carbohydrates, followed by proteins, while the mineral component and fats were much less presented. Among analyzed elements, the most prevalent was potassium with an average concentration of 26,409 mg/kgDW and the least represented was lead with an average concentration of 0.45 mg/kgDW. The main part of the mineral spectrum consisted of three elements: K, P and Ca, with a share of 95.3 %. In ascocarps of summer truffle, 24 fatty acids were detected, of which 13 were saturated and 11 unsaturated. The ratio of saturated to unsaturated fatty acids was 58.34 % to 41.66 %. The most common among saturated fatty acids was palmitic with a share of 25.89 %, while the most common unsaturated fatty acid was elaidic with a share of 28.17 %. No ingredients have been found in the fruiting bodies that would make them unfit for human consumption in any way
在训练有素的狗的帮助下,我们在波斯尼亚和黑塞哥维那(B&H)希波沃镇附近海拔 840-850 米的石灰质土壤上的天然橡树林和山毛榉林中发现了夏松露(Tuber aestivum Vittad.)的子座。 到目前为止,波黑还没有人研究过夏季松露的化学成分。这项工作的目的是分析新鲜采集的块菌的营养价值、元素组成和脂肪酸种类,并将分析结果与其他一些地方发现的夏松露块菌的相关文献结果进行比较,然后再与其他种类的块菌的子实体以及一些地面食用菌的子实体进行比较。 化学分析显示,块菌含有约 75.5% 的水分和约 25.5% 的干物质。最常见的化合物是碳水化合物,其次是蛋白质,而矿物质成分和脂肪的含量则少得多。在分析的元素中,含量最高的是钾,平均浓度为 26 409 毫克/千克DW,含量最低的是铅,平均浓度为 0.45 毫克/千克DW。矿物质光谱的主要部分由三种元素组成:钾、磷和钙,所占比例为 95.3%。在夏季松露的腹果中,检测到 24 种脂肪酸,其中 13 种是饱和脂肪酸,11 种是不饱和脂肪酸。饱和脂肪酸与不饱和脂肪酸的比例为 58.34 % 对 41.66 %。饱和脂肪酸中最常见的是棕榈酸,占 25.89%,而不饱和脂肪酸中最常见的是绵羊酸,占 28.17%。未在子实体中发现任何不适合人类食用的成分
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引用次数: 0
The effect of vermicompost and K+amino on the winter rape growth 蛭石堆肥和 K+ 氨基酸对冬油菜生长的影响
Pub Date : 2023-11-30 DOI: 10.21303/2504-5695.2023.003160
M. Olle
Vermicompost contains a high concentration of macro- and micronutrients, vitamins, growth hormones, enzymes such as proteases, amylases, lipases, cellulases, and chitinases, as well as immobilized microflora. Vermicompost improves plant germination, growth, biomass, and yield. K+amino is a biologically active liquid soil amendment that is suitable for organic gardens of all types. It is a 100 % veganic growth- and health-promoting liquid microbial inoculant for all plant types, particularly fast-growing crops like vegetables, fruits, and blooms. The purpose of investigation was to look the effect of vermicompost and k+amino on the winter rape germination and growth. Methods: There were six treatments: 1. A rape (means a rape crop in Aru Agricultural Ltd. soil), 2. A rape+YaraBela AXAN 0.3g per pot, 3. A rape+vermicompost 7.9 g per pot in the soil+the vermiextract is sprayed on the plants 1 week after sowing, 2 weeks after sowing, both treatments 1:30, 33 ml extract per 1 L water, 4. A rape+vermicompost 7.9 g per pot in the soil, 5. A rape+vermiextract is sprayed on the plants 1 week after sowing, 2 weeks after sowing, both treatments 1:30, 33 ml extract per 1 L water, 6. A rape+K+Amino biostimulator is sprayed on the plants 1 week after sowing, 2 weeks after sowing, both treatments 5 ml K+Amino per 1 L water. Results: Rape plants were elongated in treatment with YaraBela AXAN. Stem diameter was largest in K+Amino treatment. Root length was lowest in treatment with vermicompst extract (soil was too moisty and compacted). Conclusions: Vermicompost tended to improve plant growth, while stem diameter was largely increased by using k+amino fertilizer. It turns out both actually are good sources of mineral nutrients and also different enzymes and microbes, which help plants grow
蛭石堆肥含有高浓度的宏量和微量营养元素、维生素、生长激素、蛋白酶、淀粉酶、脂肪酶、纤维素酶和几丁质酶等酶类以及固定微生物菌群。蛭石堆肥可提高植物的发芽率、生长率、生物量和产量。K+amino 是一种具有生物活性的液体土壤改良剂,适用于各种类型的有机花园。它是一种 100% 纯素的促进生长和健康的液态微生物接种剂,适用于所有类型的植物,尤其是蔬菜、水果和花卉等快速生长的作物。调查的目的是了解蛭石堆肥和 k+amino 对冬油菜发芽和生长的影响。研究方法共有六个处理:1.油菜(指在阿鲁农业有限公司土壤中种植的油菜作物);2.油菜+YaraBela AXAN,每盆 0.3 克;3.油菜+蛭石,每盆 7.9 克;4.油菜+蛭石,每盆 7.9 克;5.油菜+蛭石,每盆 7.9 克;6.油菜+蛭石,每盆 7.9 克;7.油菜+蛭石,每盆 7.9 克;8.油菜+蛭石,每盆 7.9 克;9.油菜+蛭石,每盆 7.9 克;10.油菜+蛭石,每盆 7.9 克。播种 1 周后、2 周后分别向植株喷洒油菜+藜芦提取物,两种处理的比例均为 1:30,每 1 升水 33 毫升提取物;6. 播种 1 周后、2 周后分别向植株喷洒油菜+钾+氨基生物刺激剂,两种处理的比例均为每 1 升水 5 毫升钾+氨基。结果在使用雅苒贝拉 AXAN 的处理中,油菜植株变长。茎直径在 K+Amino 处理中最大。使用蛭石提取物的处理中根系长度最小(土壤过于湿润和板结)。结论蛭石往往能促进植物生长,而使用 K+ 氨基肥料则能在很大程度上增加茎直径。事实证明,这两种肥料实际上都是矿物质养分的良好来源,同时还含有不同的酶和微生物,有助于植物生长。
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引用次数: 0
Hydropriming improves seed germination in horned melon (Cucumis metuliferus E. Mey. Ex Naudin) landraces 水刺法能提高角瓜(Cucumis metuliferus E. Mey. Ex Naudin)陆生品系的种子萌发率
Pub Date : 2023-11-30 DOI: 10.21303/2504-5695.2023.003038
M. Mutetwa, Paul Chaibva, Ignatius Chagonda, V. Makuvaro, T. Mtaita, W. Ngezimana, J. Masaka, Pepukai Manjeru
The global popularity of horned melon highlights the need of sound crop establishment processes, since uniform and quick seed germination has a substantial influence on crop yield and quality. Using a Completely Randomised Design (CRD) with a factorial structure, the effects of hydro priming durations (0, 8, 16, 24, 32, and 40 h) on seeds of two horned melon landrace (L1&L2) were investigated. Hydro priming had significant (P>0.05) influence on several germination indices (germination percentage, relativized percentage, mean germination rate, germination index, synchronization, duration to 50 % germination, mean daily germination, peak value, and germination value). Among the germination indicators tested, landrace (L2) exhibited a considerably stronger germination response than L1. The findings also reveal a curvilinear relationship between priming length and germination percentage, with 24 hours being greatest proportionately. The mean germination rate, germination index, mean daily germination, peak value of germination, and germination value all rose as the hydro priming duration increased. Primed seeds germinated more quickly than unprimed ones. With increasing priming duration, the synchronization index fell linearly. The interaction between landraces and hydro priming was not significant (P>0.05) for final seed germinated, relativized germination, and synchronization indices, but it had a significant impact on all other indices. Because the efficacy of hydro priming tends to lessen with extended soaking, we recommend using it for a maximum of 32 hours. Future study should concentrate on alternative priming chemicals and the applicability of various priming solutions for various agroecosystems
角瓜在全球广受欢迎,这凸显了合理的作物种植过程的必要性,因为均匀、快速的种子萌发对作物的产量和质量有着重大影响。采用完全随机设计(CRD)的因子结构,研究了水力催芽持续时间(0、8、16、24、32 和 40 小时)对两个角瓜品种(L1 和 L2)种子的影响。水力催芽对几项发芽指标(发芽率、相对发芽率、平均发芽率、发芽指数、同步性、发芽率达到 50%的持续时间、平均日发芽率、峰值和发芽值)有显著影响(P>0.05)。在测试的发芽指标中,陆地品系(L2)的发芽反应明显强于陆地品系(L1)。研究结果还揭示了引种时间与发芽率之间的曲线关系,其中 24 小时的比例最大。平均发芽率、发芽指数、平均日发芽率、发芽峰值和发芽值都随着水引种时间的增加而上升。经催芽的种子比未经催芽的种子发芽更快。随着水力催芽持续时间的延长,同步指数呈线性下降。对于最终种子发芽率、相对发芽率和同步指数而言,土地品种与水力催芽之间的交互作用不显著(P>0.05),但对所有其他指数都有显著影响。由于水力催芽的效果会随着浸泡时间的延长而减弱,我们建议最多使用 32 小时。今后的研究应集中在替代打底化学品以及各种打底解决方案在不同农业生态系统中的适用性上。
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引用次数: 0
Assessment of the quality of cream-white candy with the addition of fruit and berry paste during storage 评估添加水果和浆果糊的乳白色糖果在储存期间的质量
Pub Date : 2023-11-30 DOI: 10.21303/2504-5695.2023.003193
O. Shydakova-Kameniuka, A. Zagorulko, K. Kasabova, A. Zahorulko
One of the trends in modern nutrition is targeting people who care about their health. Confectionery made from sugar is in stable demand among people of different age groups, and research into their enrichment with vital nutrients is promising. One of the ways to implement such a task is the use of fruit and berry processing products, in particular in the form of pastes, during the production of candies. The purpose of the research was to evaluate the quality of cream-whipped candies with the addition of apple, quince and blackcurrant paste (40: 50: 10) during storage. It was established that the process of hydrolysis of the lipid component of candies with paste occurs at a higher rate compared to the control. This is due to their higher acidity and moisture. However, the fats of candies with the addition of fruit and berry paste are less prone to the formation of peroxides, which is explained by the presence in their composition of a significant number of polyphenolic compounds with pronounced antioxidant properties (anthocyanins and catechins), which are absent in the control sample. In particular, on the 60th day of storage, the value of the peroxide value for the sample with the additive was 27.9 % less than that of the control product. It has been established that candies with paste lose moisture more slowly due to the higher content of non-starch polysaccharides. Thus, on the 60th day, the moisture content of the control decreased by 11.33 %, and the product with paste decreased by 8.72 %. Smaller moisture losses ensure greater stability of their rheological characteristics during storage. It was noted that the strength and density of such candies increase more slowly than in the sample without the additive. The results of the research indicate the compliance of cream-whipped candies with fruit and berry paste to the normative storage terms in terms of oxidative stability of the lipid complex, physico-chemical and sensory indicators
现代营养学的趋势之一是以关注健康的人群为目标。不同年龄段的人对糖制糖果的需求量都很稳定,而对其富含重要营养成分的研究也很有前景。实现这一任务的方法之一是在糖果生产过程中使用水果和浆果加工产品,特别是糊状产品。这项研究的目的是评估添加了苹果、榅桲和黑加仑果酱(40:50:10)的奶油发泡糖果在储存期间的质量。 结果表明,与对照组相比,加了糊状物的糖果中脂质成分的水解率更高。这是因为它们的酸度和水分较高。不过,添加了水果和浆果糊的糖果脂肪不易形成过氧化物,这是因为其成分中含有大量具有明显抗氧化特性的多酚化合物(花青素和儿茶素),而对照样品中不含有这些物质。特别是在储存的第 60 天,添加了添加剂的样品的过氧化值比对照产品低 27.9%。 已经证实,由于非淀粉多糖含量较高,糊状糖果的水分流失更慢。因此,在第 60 天时,对照组产品的水分含量减少了 11.33%,而添加了糊状物的产品则减少了 8.72%。较小的水分损失可确保贮藏期间流变特性的稳定性。与不添加添加剂的样品相比,这种糖果的强度和密度增加得更慢。 研究结果表明,含水果和浆果糊的奶油发泡糖果在脂质复合物的氧化稳定性、理化指标和感官指标方面都符合标准储存条件。
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引用次数: 0
Traditional cereal-based dishes of the Newari community of Nepal and their preparation process 尼泊尔纽瓦瑞社区的传统谷物菜肴及其制作过程
Pub Date : 2023-11-30 DOI: 10.21303/2504-5695.2023.003192
Rukmani Pache Magar, Grishma Shrestha, Kamana Kandel, Bijaya Lamichhane, Bidhya Laxmi Thapa, Bishal Thagunna
Around the world, cereal grains provide a significant source of dietary nutrients. Since prehistoric times, cereal-based food products have formed the foundation of the human diet. Fermented foods made from cereals account for a significant portion of the calorie intake in developing nations. Worldwide dietary recommendations are urging the consumption of whole grains due to mounting evidence that these grains and food products made from them can improve health in ways other than just giving energy and minerals. The creation of novel food items with qualities that improve health will be aided by the understanding gained from the functional qualities of the many chemical components found in whole grains. Therefore, the main aim of this study is to illuminate the varied application of cereals like rice, maize, wheat, and barley in preparing a wide range of delightful dishes like (Aila, Dhindo, Yomari, Selroti, Chatamari, Khurma, Chiura, and Lakhamari) which holds immense significance in shaping the cultural identity of the Newari community. In this study, the nutritional advantages of cereals highlight their crucial role in maintaining the health and well-being of the Newar community. Cereals are abundant in essential vitamins, fibers, minerals, and carbohydrates as a fundamental component of a well-balanced diet that promotes overall well-being
在世界各地,谷物都是膳食营养的重要来源。自史前时代起,谷物食品就成为人类饮食的基础。在发展中国家,谷物制成的发酵食品占热量摄入的很大一部分。有越来越多的证据表明,全谷物及其制成的食品不仅能提供能量和矿物质,还能在其他方面改善健康状况,因此全世界的膳食建议都敦促人们食用全谷物食品。从全谷物中发现的多种化学成分的功能特性中获得的认识,将有助于创造出具有改善健康品质的新型食品。 因此,本研究的主要目的是阐明大米、玉米、小麦和大麦等谷物在制作各种美味佳肴中的不同应用,如(艾拉、丁多、约马利、塞洛蒂、查塔马利、胡尔马、奇乌拉和拉卡马利),这些菜肴对塑造纽瓦瑞社区的文化特征具有重要意义。在这项研究中,谷物的营养优势凸显了其在维护纽瓦族人健康和福祉方面的重要作用。谷物富含人体必需的维生素、纤维、矿物质和碳水化合物,是促进整体健康的均衡饮食的基本组成部分
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引用次数: 0
Proteomic analysis of higher & lower altitude cultivars of Coffea arabica reveals differences related to environmental adaptations and coffee bean flavour. 蛋白质组学分析;低海拔阿拉比卡咖啡品种揭示了与环境适应和咖啡豆风味有关的差异。
Pub Date : 2023-11-06 DOI: 10.29173/eureka28796
Caitlin Fenrich, Phil Lauman, Prabashi Wickramasinghe
Coffee ranks among the most popular beverages worldwide and is an important commodity in developing nations. While coffee beans harvested from Coffea arabica are considered to have a superior rich and balanced flavour, they are susceptible to disease and climatic variables like temperature, precipitation, and oxygen availability, each of which varies with altitude. We performed a comprehensive proteomic comparison of two C. arabica cultivars, the high-altitude Rwanda Shyira (RS) and the lower-altitude Brazil Flor de Ipe (BFDI), using liquid chromatography MS/MS analysis. Five of the identified 531 proteins exhibited statistically significant differences in expressional intensity between the two cultivars. These differences may correspond to bitter flavonoid concentrations along with adaptations to cold, hypoxic, and disease stressors at different altitudes and geographic niches. These substantial proteomic differences identified between these elevations provide a greater understanding of the effects of altitude on the C. arabica plant and its coffee, which has implications for the global market.
咖啡是世界上最受欢迎的饮料之一,也是发展中国家的重要商品。虽然从阿拉比卡咖啡豆中收获的咖啡豆被认为具有优越的丰富和平衡的味道,但它们容易受到疾病和气候变量的影响,比如温度、降水和氧气的可用性,这些都随着海拔的变化而变化。采用液相色谱-质谱/质谱分析方法,对高海拔的卢旺达Shyira (RS)和低海拔的巴西Flor de Ipe (BFDI)两种阿拉比卡咖啡品种进行了蛋白质组学比较。所鉴定的531个蛋白中,有5个蛋白在两个品种之间的表达强度存在统计学差异。这些差异可能对应于苦味类黄酮浓度以及对不同海拔和地理位置的寒冷、缺氧和疾病压力源的适应。这些海拔高度之间确定的蛋白质组学差异提供了对海拔对阿拉比卡咖啡植物及其咖啡的影响的更好理解,这对全球市场具有重要意义。
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引用次数: 0
Effect of storage conditions on the physical properties of coffee beans with different qualities 贮存条件对不同品质咖啡豆物理性质的影响
Pub Date : 2023-09-25 DOI: 10.21303/2504-5695.2023.003008
Gabriel Carvalho Matias, Ana Paula de Carvalho Alves, Flávio Meira Borem, Luana Haeberlin, Claudia Mendes dos Santos, Sttela Dellyzete Veiga Franco da Rosa
The quality of coffee starts in the field, and goes through the harvesting and post-harvesting processes, and continues to storage and transportation. The storage of coffee beans aims to stock a product for a certain period of time. However, factors such as quality and metabolism of the grain, water content, environmental conditions of the warehouse, type of packaging, and storage time influence the maintenance of these characteristics, and may negatively affect sensory aspects of the beverage. The use of high-barrier packaging or refrigerated storage practices are alternatives that can ensure grain quality during storage. Thus, the objective of the present study was to evaluate the behavior of raw coffee beans, of different processing and quality levels, packed in impermeable packages and stored in a refrigerated environment. The specialty coffees were previously sampled and characterized as to the drink, with two lots (one of natural coffee and the other of pulped natural coffee) evaluated with a score of 82 points, and another lot of natural coffee evaluated with a score of 84 points. They were packed in two types of packaging: moisture and gas permeable, and gas, moisture, and light impermeable. The beans were stored in ambient conditions without temperature control and in refrigerated environments. The CO2 concentration inside the packaging was measured, and the physical analyses – water content, color, and apparent specific mass – were performed. According to the results there is less variation in the water content and coloration of the grains stored in high barrier packages. The storage of the beans in refrigerated condition and in high barrier packing is efficient in the retarding of the loss of quality of the coffees during the nine months of the experiment
咖啡的品质从田间开始,经过采收和采收后的过程,并持续储存和运输。咖啡豆储存的目的是将一种产品储存一段时间。然而,谷物的品质和新陈代谢、含水量、仓库的环境条件、包装类型和储存时间等因素会影响这些特性的保持,并可能对饮料的感官方面产生负面影响。使用高阻隔包装或冷藏储存方法是可以确保储存期间粮食质量的替代方法。因此,本研究的目的是评估不同加工工艺和质量水平的生咖啡豆在不透水包装和冷藏环境中储存的行为。精品咖啡之前被抽样并被描述为饮料,两批(一种是天然咖啡,另一种是天然咖啡浆)的评估得分为82分,另一批天然咖啡的评估得分为84分。它们被包装在两种类型的包装:透湿和透气,气体,潮湿,和不透光。这些豆子被储存在没有温度控制的环境条件和冷藏环境中。测量了包装内的二氧化碳浓度,并进行了物理分析-含水量,颜色和表观比质量。结果表明,储存在高阻隔包装中的谷物的含水量和颜色变化较小。在长达9个月的实验中,将咖啡豆冷藏和高阻隔包装有效地延缓了咖啡品质的下降
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引用次数: 0
Consumers’ safety of some selected ready to eat and street vended foods in Wudil, Kano state as determined by microbial content 根据微生物含量测定卡诺州乌迪尔市一些选定的即食食品和街头摊贩食品的消费者安全性
Pub Date : 2023-09-25 DOI: 10.21303/2504-5695.2023.002921
Abubakar Ibrahim Garba
Because of their cost, affordability, availability and simplicity to prepare, ready-to-eat foods are highly consumed in all over Nigeria, therefore critical to Nigerians’ health. This research aimed at ascertaining the consumers’ safety in terms of microbial load in ready-to-eat sell on the street Awara (Steamed Cowpea Beans Slurry) and Moin-moin (Bean pie) sold in three different vending locations retailed to the students of Aliko Dangote University of Science and Technology Wudil, Kano state Nigeria. Total of 24 samples consisting of equal quantity of Awara (Bean pie) and Moin-moin (Steamed Cowpea Bean Slurry) were collected from three different location in Wudil town and analyzed for total aerobic bacteria, fungi and Staphyococcus count. Seven different species of microorganism of health concern were identified to include Micrococcus spp, Staphylococcus aureous, Bacillus spp., Klebsiella spp., Mucor, Rhizophus stoonifer and Aspergillus flavus. The total mean of the aerobic bacteria, fungi and Staphylococcus count across all the location in all the samples were found to be withing the tolerable range with the standard set by the International Commission for Microbial Specification for Foods (ICMSF) and Food and Environmental Hygiene Standard (FEHD) as well as the South Wales Food Authority Standards for ready-to-eat foods. Higher microbial counts were founds on Moin-moin sample and this may be attributed to its high moisture content which is found to range from 64.2 to 70.0 %. The comparison of this research finding with international standard justified the safety of consumers and concludes the acceptability of these foods as safe to eat even though the presence of microorganisms such as Aspergilus spp. has been found to pose serious public health issue such as aflatoxin intoxication
由于其成本高、价格可承受、可获得性和制作简单,尼日利亚各地的即食食品消费量很大,因此对尼日利亚人的健康至关重要。本研究旨在确定消费者在街头出售的Awara(蒸豇豆浆)和Moin-moin(豆饼)的微生物负荷方面的安全性,这些食品在三个不同的自动售货点出售,零售给尼日利亚卡诺州乌迪尔的Aliko Dangote科技大学的学生。从乌迪勒镇三个不同地点采集等量的Awara(豆饼)和Moin-moin(蒸豇豆泥)共24份样品,分析需氧细菌、真菌和葡萄球菌总数。鉴定出7种具有卫生意义的微生物,包括微球菌、金黄色葡萄球菌、芽孢杆菌、克雷伯氏菌、毛霉菌、褐根菌和黄曲霉。所有样本在所有地点的需氧细菌、真菌及葡萄球菌总数的平均值,均符合国际食物微生物规格委员会(ICMSF)、食物及环境卫生标准(食环署)及南威尔士食物管理局即食食物标准所订定的容许范围。在Moin-moin样品中发现了较高的微生物计数,这可能归因于其高水分含量,其范围为64.2%至70.0%。将这一研究结果与国际标准进行比较,证明了消费者的安全性,并得出结论,即使发现曲霉等微生物的存在会造成黄曲霉毒素中毒等严重的公共卫生问题,这些食品也可以安全食用
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引用次数: 0
Insights on the potential of RNA-Seq on improving pomological traits of African indigenous fruit trees: a mini review RNA-Seq技术在改善非洲本土果树果学性状方面的研究进展
Pub Date : 2023-09-18 DOI: 10.21303/2504-5695.2023.003033
Paul Chaibva, Edith Mugehu, Pepukai Manjeru
Fruit tree improvement has taken great strides by roping in improved and efficient biotechnological tools to increase fruit yield and quality to meet local and export demands. For the past decade, the RNA-Seq tool has successfully been used in fruit tree improvement programs to identify genes, dissect complex traits, and understand different molecular pathways and differential expression of genes. However, despite their growing importance in food and nutrition security, medicinal uses, and climate change mitigation strategies, very little has been done to improve the pomological traits of African indigenous fruits, especially at the molecular level. African indigenous fruit trees exhibit unexplained variation in flowering, fruit load, fruit size, fruit ripening, fruit taste, fruit nutritional composition and shelf-life. The booming local commercial companies and export markets are demanding consistent quality indigenous fruits. This has necessitated the need for fast and effective tools that will hasten the understanding and improvement of fruiting qualities. The improvement of fruiting and fruit qualities will go a long way in accelerating the domestication and commercialization of African indigenous fruit trees. This review paper gives molecular biology insights on how RNA-Seq has been successfully used in fruit improvement of exotic fruits through gene identification, comparative transcriptome analysis under different conditions, and understanding molecular pathways that influence important pomological traits. The review article also unearths opportunities where RNA-Seq can improve our knowledge and improvement of undesirable traits common in African indigenous fruits
果树改良取得了巨大进展,引进了改良和高效的生物技术工具,提高了果实产量和质量,以满足当地和出口的需求。在过去的十年中,RNA-Seq工具已成功地用于果树改良项目,以鉴定基因,解剖复杂性状,并了解不同的分子途径和基因的差异表达。然而,尽管它们在粮食和营养安全、医药用途和减缓气候变化战略方面日益重要,但在改善非洲本土水果的果学特征方面,特别是在分子水平上,所做的工作却很少。非洲本土果树在开花、果实负荷、果实大小、果实成熟、果实味道、果实营养成分和保质期方面表现出无法解释的差异。蓬勃发展的当地商业公司和出口市场要求质量稳定的本土水果。这就需要有快速和有效的工具来加速了解和改进果实质量。结果和果实品质的改善将大大加快非洲本土果树的驯化和商业化。本文从基因鉴定、不同条件下的比较转录组分析以及影响重要果学性状的分子途径等方面,对RNA-Seq技术如何成功应用于外来水果的果实改良进行了分子生物学研究。这篇综述文章还揭示了RNA-Seq可以提高我们对非洲本土水果常见不良性状的认识和改进的机会
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引用次数: 0
Different nursery growing systems and growth media improved germination and seedling growth of fever tea (Lippia javanica) 不同育苗制度和培养基对热茶种子萌发和幼苗生长的影响
Pub Date : 2023-09-18 DOI: 10.21303/2504-5695.2023.003040
Ignatius Chagonda, Paul Chaibva, Nigel Ntini, Munyaradzi Gwazane, Blessing Ndau, Wonder Ngezimana, Taurira Mtaita, Moses Mutetwa
An experiment was set up in a Completely Randomized Design (CRD) to examine the influence of nursery growing methods and growth medium on fever, tea germination, and seedling development. Two nursery growth methods (Conventional-CON and Float tray system FTs) and six different growing medium (pine bark, peat moss, coco peat, vermiculite, sand, and cattle manure) were used. ANOVA was used to compare treatment means, and means were separated using the Least Significant Difference (LSD) at a 5 % significance level. The nursery growing method and growth material exhibited a strong interaction regarding the germination index, germination percentage, seedling height, leaf area, root length, density, and final crop stand. The float tray approach yielded the greatest germination index for peat moss and the lowest for cattle manure. In the float tray method, peat moss and coco peat had the highest germination percentages, whereas cattle manure had the lowest. Under the float tray technique, the tallest seedlings emerged in peat moss and the smallest in coco peat. Peat moss had the most leaf area in the float tray system, whereas coco peat had the least in the conventional technique. Peat moss in the float tray system had the highest root length density while coco peat in the conventional technique had the lowest. Peat moss in the float tray system had the highest final crop stand, whereas seedlings in the conventional and float tray systems had nothing. based on the findings, it is recommended that fever tea seedlings be grown on peat moss using the float tray method
采用完全随机设计(CRD),研究苗圃栽培方式和培养基对茶树发热、发芽率和幼苗发育的影响。采用两种苗圃生长方法(Conventional-CON和Float tray system FTs)和6种不同的生长介质(松皮、泥炭苔藓、椰子泥炭、蛭石、沙子和牛粪)。采用方差分析(ANOVA)比较治疗方法,在5%的显著性水平上使用最低显著性差异(Least Significant Difference, LSD)进行分离。苗圃生长方式和生长材料在发芽指数、发芽率、苗高、叶面积、根长、密度和最终林分等方面表现出较强的交互作用。浮盘法泥炭苔藓萌发指数最高,牛粪萌发指数最低。浮盘法发芽率最高的是泥炭苔和椰泥,发芽率最低的是牛粪。在浮盘法下,泥炭苔苗最高,可可泥炭苗最小。浮盘系统中泥炭苔的叶面积最大,而常规系统中可可泥炭的叶面积最小。浮盘法的泥炭苔根长密度最高,常规法的可可泥炭根长密度最低。浮盘系统中泥炭苔藓的最终林分最高,而传统和浮盘系统中的幼苗则没有。在此基础上,建议采用浮盘法在泥炭苔上种植热茶苗
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EUREKA: Life Sciences
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