Pub Date : 2023-05-15DOI: 10.21303/2504-5695.2023.002906
Daniel Thakuma Tizhe, Dentsen Fortune Dashe, J. Kwaga
Dengue is a disease caused by dengue virus. It is a major mosquito-borne viral disease of humans that is endemic in areas of tropical and subtropical regions, which are environmentally suitable for vector propagation. The disease poses a major threat to the overwhelmed and weak public healthcare delivery system in the developing world, especially in Africa where febrile illnesses are common. The disease undermines the fight against febrile illnesses as infection with dengue often remains undetected or misdiagnosed as malaria or other febrile diseases. This review article highlights dengue epidemiology in Nigeria, dengue transmission dynamics, pathogenesis, diagnosis and current interventions strategies, challenges in addressing dengue infection and future prospects towards dengue elimination. The literature search for publications on dengue virus infection in Nigeria was performed using Google Scholar, PubMed, Web of Science, African Journals Online and other scholarly online databases. Dengue remains a threat to Nigeria and other African countries. In Nigeria, the occurrence of this infection remains a low priority in the public health sector even though it has been reported in about 17 states across the country. The reports of dengue infection in all the geo-political zones suggests active transmission of dengue, hence the need to consider other etiologies of febrile illnesses and engage the public to sustain local involvement as local risk to dengue outbreaks is linked to the population’s knowledge, attitude and behavioral practices that encourage vector breeding
登革热是一种由登革热病毒引起的疾病。它是一种主要的人类蚊媒病毒性疾病，在热带和亚热带地区流行，这些地区的环境适合媒介传播。这种疾病对发展中国家不堪重负和薄弱的公共卫生保健提供系统构成重大威胁，特别是在发热性疾病普遍存在的非洲。该病破坏了防治发热性疾病的斗争，因为登革热感染往往未被发现或被误诊为疟疾或其他发热性疾病。这篇综述文章重点介绍了尼日利亚的登革热流行病学、登革热传播动力学、发病机制、诊断和当前干预策略、应对登革热感染的挑战以及消除登革热的未来前景。利用Google Scholar、PubMed、Web of Science、African Journals Online和其他学术在线数据库对尼日利亚登革热病毒感染出版物进行了文献检索。登革热仍然对尼日利亚和其他非洲国家构成威胁。在尼日利亚，尽管全国约有17个州报告了这种感染，但这种感染的发生仍然是公共卫生部门的低优先事项。所有地缘政治区域的登革热感染报告表明登革热传播活跃，因此需要考虑发热性疾病的其他病因，并让公众保持当地参与，因为当地爆发登革热的风险与居民鼓励媒介繁殖的知识、态度和行为做法有关
{"title":"Dengue fever in Nigeria: a mini review","authors":"Daniel Thakuma Tizhe, Dentsen Fortune Dashe, J. Kwaga","doi":"10.21303/2504-5695.2023.002906","DOIUrl":"https://doi.org/10.21303/2504-5695.2023.002906","url":null,"abstract":"Dengue is a disease caused by dengue virus. It is a major mosquito-borne viral disease of humans that is endemic in areas of tropical and subtropical regions, which are environmentally suitable for vector propagation. The disease poses a major threat to the overwhelmed and weak public healthcare delivery system in the developing world, especially in Africa where febrile illnesses are common. The disease undermines the fight against febrile illnesses as infection with dengue often remains undetected or misdiagnosed as malaria or other febrile diseases. This review article highlights dengue epidemiology in Nigeria, dengue transmission dynamics, pathogenesis, diagnosis and current interventions strategies, challenges in addressing dengue infection and future prospects towards dengue elimination. The literature search for publications on dengue virus infection in Nigeria was performed using Google Scholar, PubMed, Web of Science, African Journals Online and other scholarly online databases. Dengue remains a threat to Nigeria and other African countries. In Nigeria, the occurrence of this infection remains a low priority in the public health sector even though it has been reported in about 17 states across the country. The reports of dengue infection in all the geo-political zones suggests active transmission of dengue, hence the need to consider other etiologies of febrile illnesses and engage the public to sustain local involvement as local risk to dengue outbreaks is linked to the population’s knowledge, attitude and behavioral practices that encourage vector breeding","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72600716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.21303/2504-5695.2023.002905
H. Bishop, H. Inabo, Elijah Ekah Ella, Mohammed Bello
Improper waste disposal, unsafe water and indiscriminate water-contact activities are major factors enhancing continuous spread of schistosomiasis in Nigeria. Many water bodies are prone to contamination with human wastes directly discharged into them or due to surface runoff, and are infested with parasites. Open defecation and discharge of household sewage into water channels is still practiced. Children conduct activities in these water bodies, thereby exposing themselves to infections with schistomes among other pathogens. Urine samples (10 mL each) were collected from 600 consented school adolescents across six Local Government Areas of Kaduna State, Nigeria. Information on their water-contact activities were obtained by means of questionnaires. Urine sediment was examined for Schistosoma haematobium eggs by microscopy. No infection was recorded in adolescents who had awareness about the disease. Those who engaged in swimming (9.2 %, OR=2.2) and fishing (10.3 %, OR=2.1) were significantly more infected than those who did not (P≤0.05). Adolescents who worked on irrigated farms (9.0 %, OR=1.4), washed clothes in rivers (9.0 %, OR=1.6), or fetch water from rivers for domestic purpose (10.0 %) were more infected than others who did not engage in those activities. Therefore, swimming and fishing are important factors enhancing the spread of schistosomiasis among school adolescents in Kaduna State. Irrigation farming, washing of clothes in rivers or fetching water from rivers exposed the adolescents to schistosome infections. Widespread awareness campaigns, provision safe water to communities, and standard water-based recreational centers are paramount
{"title":"Urinary schistosomiasis: risk factors and symptoms among school adolescents in Kaduna State, Nigeria","authors":"H. Bishop, H. Inabo, Elijah Ekah Ella, Mohammed Bello","doi":"10.21303/2504-5695.2023.002905","DOIUrl":"https://doi.org/10.21303/2504-5695.2023.002905","url":null,"abstract":"Improper waste disposal, unsafe water and indiscriminate water-contact activities are major factors enhancing continuous spread of schistosomiasis in Nigeria. Many water bodies are prone to contamination with human wastes directly discharged into them or due to surface runoff, and are infested with parasites. Open defecation and discharge of household sewage into water channels is still practiced. Children conduct activities in these water bodies, thereby exposing themselves to infections with schistomes among other pathogens. Urine samples (10 mL each) were collected from 600 consented school adolescents across six Local Government Areas of Kaduna State, Nigeria. Information on their water-contact activities were obtained by means of questionnaires. Urine sediment was examined for Schistosoma haematobium eggs by microscopy. No infection was recorded in adolescents who had awareness about the disease. Those who engaged in swimming (9.2 %, OR=2.2) and fishing (10.3 %, OR=2.1) were significantly more infected than those who did not (P≤0.05). Adolescents who worked on irrigated farms (9.0 %, OR=1.4), washed clothes in rivers (9.0 %, OR=1.6), or fetch water from rivers for domestic purpose (10.0 %) were more infected than others who did not engage in those activities. Therefore, swimming and fishing are important factors enhancing the spread of schistosomiasis among school adolescents in Kaduna State. Irrigation farming, washing of clothes in rivers or fetching water from rivers exposed the adolescents to schistosome infections. Widespread awareness campaigns, provision safe water to communities, and standard water-based recreational centers are paramount","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75096246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.21303/2504-5695.2023.002841
Shitta Kefas Babale, Hasber Salim, I. Yakudima, Buda Mohammed Kabir, Rashidu Mamman, Usman Mohammed Chiroma, Shehu Kura Ibrahim
Malaria is the most significant protozoan disease in Africa and the principal vector-borne disease (VBD) in Nigeria, which is influenced by the quality of breeding habitats that are reflected through the stage preceding adult. Control of Anopheles gambiae s.l. populations through source reduction is still considered the most effective way of prevention and control, although it has proven unsustainable in Niger State. Physico-chemical cues were investigated. Samples were taken inside and outside 1m2 cages weekly by dipping and emptying the cages from May 2019 to March 2020. The data subjected to analysis of variance (one and two-way Anova). The findings revealed the mean pupae abundance (MPA), were significantly higher in Large Water bodies (624.50±217.81), and followed by Gutters (436.00±184.2) and Swamps (285.50±125.06). The mean pupae productivity (MPP), followed the order of descending rate >GT (717.50±219.38) >LW (677.21±145.10) >SW (530.40±136.97). The result also showed that emptying technique (ET) was more sufficient and reliable than dipping technique (DT). The peak abundance and productivity of the pupal stage was June to August then declined in March,2020 both habitats. However, MPP differed significantly (p<0.05) from one another across the months in all the habitat types. The physical and chemical cues of the breeding sites, varied significantly, except in temperature, total hardness, biochemical oxygen demands, conductivity, and pH in all the habitats. This study revealed high utilization of physico-chemical properties and poses increased risk of malaria. Thus, emphasis on the vector management strategies should be given specially on gutters and large water bodies as breeding habitats of malaria vectors (MV), in Niger State
{"title":"Seasonal assessment of pupal habitat productivity of malaria vector: Anopheles gambiae s.l as influence by physico-chemical conditions at selected breeding habitats in Niger, Nigeria","authors":"Shitta Kefas Babale, Hasber Salim, I. Yakudima, Buda Mohammed Kabir, Rashidu Mamman, Usman Mohammed Chiroma, Shehu Kura Ibrahim","doi":"10.21303/2504-5695.2023.002841","DOIUrl":"https://doi.org/10.21303/2504-5695.2023.002841","url":null,"abstract":"Malaria is the most significant protozoan disease in Africa and the principal vector-borne disease (VBD) in Nigeria, which is influenced by the quality of breeding habitats that are reflected through the stage preceding adult. Control of Anopheles gambiae s.l. populations through source reduction is still considered the most effective way of prevention and control, although it has proven unsustainable in Niger State. Physico-chemical cues were investigated. Samples were taken inside and outside 1m2 cages weekly by dipping and emptying the cages from May 2019 to March 2020. The data subjected to analysis of variance (one and two-way Anova). The findings revealed the mean pupae abundance (MPA), were significantly higher in Large Water bodies (624.50±217.81), and followed by Gutters (436.00±184.2) and Swamps (285.50±125.06). The mean pupae productivity (MPP), followed the order of descending rate >GT (717.50±219.38) >LW (677.21±145.10) >SW (530.40±136.97). The result also showed that emptying technique (ET) was more sufficient and reliable than dipping technique (DT). The peak abundance and productivity of the pupal stage was June to August then declined in March,2020 both habitats. However, MPP differed significantly (p<0.05) from one another across the months in all the habitat types. The physical and chemical cues of the breeding sites, varied significantly, except in temperature, total hardness, biochemical oxygen demands, conductivity, and pH in all the habitats. This study revealed high utilization of physico-chemical properties and poses increased risk of malaria. Thus, emphasis on the vector management strategies should be given specially on gutters and large water bodies as breeding habitats of malaria vectors (MV), in Niger State","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88990502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.21303/2504-5695.2023.002909
A. O. Kolawole, K. Adamu, I. Usman, Yakubu Manbe Mohammed, M. Aliyu-Paiko
Globally the need to substitute fishmeal with other animal protein sources in the fish diet has gained more attention; which is occasioned by the increasing cost of fish. A 12-week trial was conducted to ascertain the growth indices and biochemical response of hybrid catfish (Clarias gariepinus(♀) and Heterobranchus bidorsalis (♂)) fed cockroach meal supplement. A total of 800 hybrid catfish were randomly distributed into four dietary treatments (Diet A- control feed), Diet B (50 % cockroach and 50 % fishmeal), Diet C (100 % cockroach meal) and Diet D (100 % fishmeal). Each treatment was replicated with 35 fish per experimental pond in a completely randomized design. Progressive weight gain was determined bi-weekly. At the end of the study period, fishes were randomly sacrificed for proximate compositions and plasma biochemical analyses (glucose, triglyceride, cholesterol, total protein, albumin) using standard protocols. The study revealed that growth parameters (Weight gain, Average weight gain, Protein efficiency ratio, Feed Conversion rate, Specific growth rate and Survival rate (SR) were influenced by the dietary treatments (p>0.05). The highest weight gain was recorded in Diet 1 (8847.73±292.65 g) > Diet 2 (7816.33±121.71 g)>Diet 3 (7811.67±566.08 g) >Diet 4 (7022.00±44.193 g). Similarly, the best feed conversion ratio was found in fish fed Diet A (10.12±0.01),>Diet B, C and D had the same values of 0.11 g respectively. There was no significant (p>0.05) difference in plasma biochemical indicators measured when compared to the control (Diet A). Thus, indicating that all the fish were healthy and had normal physiological activities. Cockroach meal can therefore, be used as a feed supplement without having any adverse effect on the growth and well-being of hybrid catfish fingerlings
{"title":"Dietary supplement using cockroach meal: impact on growth indices and biochemical responses in hybrid catfish","authors":"A. O. Kolawole, K. Adamu, I. Usman, Yakubu Manbe Mohammed, M. Aliyu-Paiko","doi":"10.21303/2504-5695.2023.002909","DOIUrl":"https://doi.org/10.21303/2504-5695.2023.002909","url":null,"abstract":"Globally the need to substitute fishmeal with other animal protein sources in the fish diet has gained more attention; which is occasioned by the increasing cost of fish. A 12-week trial was conducted to ascertain the growth indices and biochemical response of hybrid catfish (Clarias gariepinus(♀) and Heterobranchus bidorsalis (♂)) fed cockroach meal supplement. A total of 800 hybrid catfish were randomly distributed into four dietary treatments (Diet A- control feed), Diet B (50 % cockroach and 50 % fishmeal), Diet C (100 % cockroach meal) and Diet D (100 % fishmeal). Each treatment was replicated with 35 fish per experimental pond in a completely randomized design. Progressive weight gain was determined bi-weekly. At the end of the study period, fishes were randomly sacrificed for proximate compositions and plasma biochemical analyses (glucose, triglyceride, cholesterol, total protein, albumin) using standard protocols. The study revealed that growth parameters (Weight gain, Average weight gain, Protein efficiency ratio, Feed Conversion rate, Specific growth rate and Survival rate (SR) were influenced by the dietary treatments (p>0.05). The highest weight gain was recorded in Diet 1 (8847.73±292.65 g) > Diet 2 (7816.33±121.71 g)>Diet 3 (7811.67±566.08 g) >Diet 4 (7022.00±44.193 g). Similarly, the best feed conversion ratio was found in fish fed Diet A (10.12±0.01),>Diet B, C and D had the same values of 0.11 g respectively. There was no significant (p>0.05) difference in plasma biochemical indicators measured when compared to the control (Diet A). Thus, indicating that all the fish were healthy and had normal physiological activities. Cockroach meal can therefore, be used as a feed supplement without having any adverse effect on the growth and well-being of hybrid catfish fingerlings","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88290739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chosita Norkaew, S. Roytrakul, Sawanya Charoenlappanit, Siriwan Thaisakun, Dalina Tanyong
AIMS This study attempted to explore the mechanisms involved in pinostrobin (PN)-mediated acute leukemia cell apoptosis regulated by miR-410-5p. MATERIAL AND METHODS NB4 and MOLT-4 cells were cultured and treated with PN at the IC50 concentration. Apoptosis was examined by Annexin V-FITC/PI staining. RT-qPCR was used to measure the expression of caspase-3, BAK, BCL-W, and MCL-1. The target protein of PN was identified using LC-MS/MS followed by bioinformatic analysis. TargetScan, DIANA, and miRDB were used for the prediction of miRNAs involved in the PN-induced apoptosis mechanism. miRNA mimic transfection, RT-qPCR, and western blot analysis were performed to evaluate the regulatory effect of miRNA on its target and the involvement of miRNA in apoptosis induction by PN. In addition, the synergistic effect of PN and daunorubicin (DNR) were investigated by using the MTT assay. KEY FINDINGS The results showed that PN reduced cell viability and induced apoptosis in both leukemia cell lines. From the LC-MS/MS and bioinformatics analysis, SFRP5 and miR-410-5p were selected as a potential PN target protein and miRNA, respectively. After miRNA mimic transfection, miR-410-5p, which is an onco-miRNA, was decreased and led to increased apoptosis in both cell lines, indicating that this miRNA is involved in PN-mediated apoptosis mechanisms. Moreover, PN demonstrated a synergistic effect with DNR, suggesting that PN may be used in combination with conventional chemotherapy drugs. SIGNIFICANCE PN regulates the expression of miR-410-5p and SFRP5 to promote apoptosis in acute leukemia cells. It could be developed as an alternative treatment for leukemia in the future.
{"title":"Pinostrobin induces acute leukemia cell apoptosis via the regulation of miR-410-5p and SFRP5.","authors":"Chosita Norkaew, S. Roytrakul, Sawanya Charoenlappanit, Siriwan Thaisakun, Dalina Tanyong","doi":"10.2139/ssrn.4379242","DOIUrl":"https://doi.org/10.2139/ssrn.4379242","url":null,"abstract":"AIMS\u0000This study attempted to explore the mechanisms involved in pinostrobin (PN)-mediated acute leukemia cell apoptosis regulated by miR-410-5p.\u0000\u0000\u0000MATERIAL AND METHODS\u0000NB4 and MOLT-4 cells were cultured and treated with PN at the IC50 concentration. Apoptosis was examined by Annexin V-FITC/PI staining. RT-qPCR was used to measure the expression of caspase-3, BAK, BCL-W, and MCL-1. The target protein of PN was identified using LC-MS/MS followed by bioinformatic analysis. TargetScan, DIANA, and miRDB were used for the prediction of miRNAs involved in the PN-induced apoptosis mechanism. miRNA mimic transfection, RT-qPCR, and western blot analysis were performed to evaluate the regulatory effect of miRNA on its target and the involvement of miRNA in apoptosis induction by PN. In addition, the synergistic effect of PN and daunorubicin (DNR) were investigated by using the MTT assay.\u0000\u0000\u0000KEY FINDINGS\u0000The results showed that PN reduced cell viability and induced apoptosis in both leukemia cell lines. From the LC-MS/MS and bioinformatics analysis, SFRP5 and miR-410-5p were selected as a potential PN target protein and miRNA, respectively. After miRNA mimic transfection, miR-410-5p, which is an onco-miRNA, was decreased and led to increased apoptosis in both cell lines, indicating that this miRNA is involved in PN-mediated apoptosis mechanisms. Moreover, PN demonstrated a synergistic effect with DNR, suggesting that PN may be used in combination with conventional chemotherapy drugs.\u0000\u0000\u0000SIGNIFICANCE\u0000PN regulates the expression of miR-410-5p and SFRP5 to promote apoptosis in acute leukemia cells. It could be developed as an alternative treatment for leukemia in the future.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80682843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
AIMS Medroxyprogesterone acetate (MPA) is the most common fertility-sparing treatment in patients with early-stage endometrial cancer. If MPA treatment fails, hysterectomy is recommended. Thus, there is an urgent need for novel treatment approaches for MPA-resistant endometrial cancer patients who wish to preserve their fertility. Ferroptosis is a recently discovered type of regulated cell death caused by the excessive accumulation of reactive oxygen species (ROS), followed by aberrant lipid peroxidation. Recent studies have shown that inducing ferroptosis is a potential therapeutic strategy for cancer. However, the role of ferroptosis in endometrial cancer treatment remains to be discussed. We therefore investigated the effects of ferroptosis inducers on MPA-resistant endometrial cancer cells. MAIN METHODS The levels of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4), the main mediators of ferroptosis, were examined. Cell viability was evaluated after treatment with the ferroptosis inducers sulfasalazine, erastin, or RSL3. The degree of intracellular oxidative stress after treatment with these drugs was evaluated by the glutathione level, ROS level, ferrous iron level, lipid peroxidation and changes in mitochondrial morphology. The effect of ferroptosis inducers in vivo was also examined. KEY FINDINGS The expression of SLC7A11 and GPX4 in MPA-resistant ECC-1 cells decreased in comparison to parental ECC-1 cells. Sulfasalazine, erastin, and RSL3 significantly reduced cell viability and increased intracellular oxidative stress in MPA-resistant ECC-1 cells. Ferroptosis inducers also suppressed in vivo tumor growth more effectively in MPA-resistant ECC-1. SIGNIFICANCE Treatment with ferroptosis inducers could be a novel therapeutic approach for MPA-resistant endometrial cancer.
{"title":"Medroxyprogesterone acetate-resistant endometrial cancer cells are susceptible to ferroptosis inducers.","authors":"H. Murakami, M. Hayashi, S. Terada, M. Ohmichi","doi":"10.2139/ssrn.4355249","DOIUrl":"https://doi.org/10.2139/ssrn.4355249","url":null,"abstract":"AIMS\u0000Medroxyprogesterone acetate (MPA) is the most common fertility-sparing treatment in patients with early-stage endometrial cancer. If MPA treatment fails, hysterectomy is recommended. Thus, there is an urgent need for novel treatment approaches for MPA-resistant endometrial cancer patients who wish to preserve their fertility. Ferroptosis is a recently discovered type of regulated cell death caused by the excessive accumulation of reactive oxygen species (ROS), followed by aberrant lipid peroxidation. Recent studies have shown that inducing ferroptosis is a potential therapeutic strategy for cancer. However, the role of ferroptosis in endometrial cancer treatment remains to be discussed. We therefore investigated the effects of ferroptosis inducers on MPA-resistant endometrial cancer cells.\u0000\u0000\u0000MAIN METHODS\u0000The levels of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4), the main mediators of ferroptosis, were examined. Cell viability was evaluated after treatment with the ferroptosis inducers sulfasalazine, erastin, or RSL3. The degree of intracellular oxidative stress after treatment with these drugs was evaluated by the glutathione level, ROS level, ferrous iron level, lipid peroxidation and changes in mitochondrial morphology. The effect of ferroptosis inducers in vivo was also examined.\u0000\u0000\u0000KEY FINDINGS\u0000The expression of SLC7A11 and GPX4 in MPA-resistant ECC-1 cells decreased in comparison to parental ECC-1 cells. Sulfasalazine, erastin, and RSL3 significantly reduced cell viability and increased intracellular oxidative stress in MPA-resistant ECC-1 cells. Ferroptosis inducers also suppressed in vivo tumor growth more effectively in MPA-resistant ECC-1.\u0000\u0000\u0000SIGNIFICANCE\u0000Treatment with ferroptosis inducers could be a novel therapeutic approach for MPA-resistant endometrial cancer.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81301839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. G. da Silva, K. F. Moura, A. C. de Souza, Kenny Gutemberg Nunes Silva, C. B. Vidigal, Juliana da Silva Jezuíno, Rafaella Cardoso Gravena, G. G. Pelosi, D. C. Gerardin, Maria do Carmo Franco, G. Ceravolo
AIMS This study aimed to evaluate the short- and long-term adverse effects of blood pressure (BP), vascular endothelial function, and estrogen receptor (ERα and ERβ) modulation on endothelial function in female Wistar rats treated with topiramate (TPM), an antiepileptic drug, during the peripubertal period. MATERIALS AND METHODS Female Wistar rats were treated with TPM (41 mg/kg) or water (CTR group) by gavage from postnatal day (PND) 28 to 50 (peripubertal phase). At the end of the treatment, the TPM and CTR rats were divided into two groups and evaluated after 24 h or from PND 85 (adulthood). The rats were evaluated for: thoracic aorta reactivity to phenylephrine (Phenyl), acetylcholine (ACh), and sodium nitroprusside (SNP); aortic ring reactivity after ERα and ERβ antagonism; and BP. KEY FINDINGS It was observed that vascular response to Phenyl, ACh, and SNP was similar between TPM and CTR rats in the short- and long-term evaluations. In addition, the ER antagonism did not interfere with aortic contraction or relaxation in either TPM or CTR. SIGNIFICANCE Taken together, the results show that TPM treatment during the peripubertal period does not alter aortic endothelial function and its estrogen modulation via classic ER in female Wistar rats, suggesting that TPM treatment in this period is safe for the vascular system.
{"title":"Topiramate treatment during the peripubertal period does not alter aortic endothelial function in female Wistar rats.","authors":"D. G. da Silva, K. F. Moura, A. C. de Souza, Kenny Gutemberg Nunes Silva, C. B. Vidigal, Juliana da Silva Jezuíno, Rafaella Cardoso Gravena, G. G. Pelosi, D. C. Gerardin, Maria do Carmo Franco, G. Ceravolo","doi":"10.2139/ssrn.4333542","DOIUrl":"https://doi.org/10.2139/ssrn.4333542","url":null,"abstract":"AIMS\u0000This study aimed to evaluate the short- and long-term adverse effects of blood pressure (BP), vascular endothelial function, and estrogen receptor (ERα and ERβ) modulation on endothelial function in female Wistar rats treated with topiramate (TPM), an antiepileptic drug, during the peripubertal period.\u0000\u0000\u0000MATERIALS AND METHODS\u0000Female Wistar rats were treated with TPM (41 mg/kg) or water (CTR group) by gavage from postnatal day (PND) 28 to 50 (peripubertal phase). At the end of the treatment, the TPM and CTR rats were divided into two groups and evaluated after 24 h or from PND 85 (adulthood). The rats were evaluated for: thoracic aorta reactivity to phenylephrine (Phenyl), acetylcholine (ACh), and sodium nitroprusside (SNP); aortic ring reactivity after ERα and ERβ antagonism; and BP.\u0000\u0000\u0000KEY FINDINGS\u0000It was observed that vascular response to Phenyl, ACh, and SNP was similar between TPM and CTR rats in the short- and long-term evaluations. In addition, the ER antagonism did not interfere with aortic contraction or relaxation in either TPM or CTR.\u0000\u0000\u0000SIGNIFICANCE\u0000Taken together, the results show that TPM treatment during the peripubertal period does not alter aortic endothelial function and its estrogen modulation via classic ER in female Wistar rats, suggesting that TPM treatment in this period is safe for the vascular system.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76091530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juliette is an undergraduate student in the Faculty of Science who is researching the effects of Rho protein activation on mast cells degranulation. Her image titled Mast Cell Degranulation in Response to Rho Activating Drugs is featured on the front cover of this issue.
{"title":"Student Researcher Spotlight","authors":"Juliette Eshleman","doi":"10.29173/eureka28788","DOIUrl":"https://doi.org/10.29173/eureka28788","url":null,"abstract":"Juliette is an undergraduate student in the Faculty of Science who is researching the effects of Rho protein activation on mast cells degranulation. Her image titled Mast Cell Degranulation in Response to Rho Activating Drugs is featured on the front cover of this issue.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86257761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"From Dr. Lisa Willis","authors":"L. Willis","doi":"10.29173/eureka28786","DOIUrl":"https://doi.org/10.29173/eureka28786","url":null,"abstract":"","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79294746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Matthew is an undergraduate student in the Faculty of Science who is researching the effects of functional electrical stimulation on glial cells of the nervous system. His image titled Galaxies of Glia Interface with Technology is featured on the back cover of this issue.
{"title":"Student Researcher Spotlight","authors":"Matthew Birtle","doi":"10.29173/eureka28789","DOIUrl":"https://doi.org/10.29173/eureka28789","url":null,"abstract":"Matthew is an undergraduate student in the Faculty of Science who is researching the effects of functional electrical stimulation on glial cells of the nervous system. His image titled Galaxies of Glia Interface with Technology is featured on the back cover of this issue.","PeriodicalId":11962,"journal":{"name":"EUREKA: Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72402301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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