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Evaluation of Regulatory T Cells in Chronic Hepatitis C Patients 慢性丙型肝炎患者调节性T细胞的评估
Pub Date : 2021-12-30 DOI: 10.36519/idcm.2021.68
Yesim Tuyji-Tok, A. Şener, Aysegul Aksoy-Gokmen, T. Demirdal, Selçuk Kaya
Background: Although there have been significant improvements in treatment strategies, hepatitis C infection is still among the most critical public health problems worldwide. Upon entry of hepatitis C virus (HCV) into host liver cells, many antagonist immune responses are induced, including the production of regulatory T cells (Tregs). The main function of Tregs is coordinating an appropriate immune response, including suppression of this response once it is no longer needed. Tregs protect cells from immunopathologic damage of HCVspecific T cells but, on the other hand, cause viral persistence, cirrhosis and hepatocellular carcinoma (HCC). In this study, we aimed to determine whether the evaluation of Tregs in hepatitis C patients was a useful method for indicating disease chronicity. Materials and Methods: The peripheral blood samples were taken from sixty volunteers, including 30 non-cirrhotic chronic hepatitis C patients and 30 healthy controls selected from infectious diseases outpatient/clinic service applicants. Their CD4+CD25high FoxP3+CD152+CD127low Treg distributions were measured by flow cytometry, using recently identified markers combined. Results: Treg percentages of the subjects with hepatitis C infection (7.97±1.25) were significantly higher than those of the healthy control group (4.29±1.37) (p<0.05). Additionally, the percentages of Tregs were strongly correlated with HCV RNA load (r=0.725, p<0.05) as well as serum AFP levels (r=0.711, p<0.05). Conclusion: Tregs are thought to play an important role in the immunology of HCV infection, thereby providing a promising method for the prediction of disease prognosis and for potentially developing new therapeutic strategies by targeting the Treg.
背景:尽管治疗策略有了显著的改进,但丙型肝炎感染仍然是世界范围内最严重的公共卫生问题之一。当丙型肝炎病毒(HCV)进入宿主肝细胞后,许多拮抗免疫反应被诱导,包括调节性T细胞(Tregs)的产生。Tregs的主要功能是协调适当的免疫反应,包括在不再需要这种反应时抑制这种反应。Tregs保护细胞免受hcv特异性T细胞的免疫病理损伤,但另一方面,导致病毒持续存在,肝硬化和肝细胞癌(HCC)。在这项研究中,我们旨在确定丙型肝炎患者Tregs的评估是否是一种指示疾病慢性性的有用方法。材料与方法:采集60名志愿者的外周血样本,其中30名非肝硬化慢性丙型肝炎患者和30名健康对照者,均选自传染病门诊/门诊服务申请人。用流式细胞术检测CD4+CD25high FoxP3+CD152+CD127low Treg的分布。结果:丙型肝炎感染者Treg百分比(7.97±1.25)显著高于健康对照组(4.29±1.37),差异有统计学意义(p<0.05)。此外,Tregs百分比与HCV RNA载量(r=0.725, p<0.05)和血清AFP水平(r=0.711, p<0.05)密切相关。结论:Treg在HCV感染的免疫学中发挥重要作用,为预测疾病预后提供了一种有前景的方法,并有可能开发出针对Treg的新治疗策略。
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引用次数: 0
Do the Most Cited Web Sources Provide Proper Information on Influenza Vaccination? 被引用最多的网络来源是否提供了正确的流感疫苗接种信息?
Pub Date : 2021-12-30 DOI: 10.36519/10.36519/idcm.2021.71
E. M. Saricaoğlu, Ayda Gholizadeh, Cemre Kırım, Elif Yunt, Ihsan Mert Turk, İlker Anıl Celik, Kardelennur Yıldırım, Pelin Ozbakir, A. Azap
Objective: The Internet is increasingly preferred as a source of health-related information. However, several studies revealed deficiencies in the quality of web-based health information. Therefore, this study aimed to analyze the content and design of influenza vaccine-related websites. Materials and Methods: This study was conducted using a search engine, Google Search, in the Turkish language. We identified 15 influenza-related search terms, and for each term, the top ten links on the first page were included in the study after removing duplicate links. Then, we evaluated identified websites in terms of content and design criteria. Results: Seventy-one links related to influenza vaccination were evaluated in our study. The mean content criteria score of all selected websites was 11.5 points. While academic websites received the highest contents point with 22.5, news and hospital sites remained under the average with 10.5 and 8.9 points, respectively. Thirty-eight per cent of the websites had aesthetic/content professionalism, and 77.5% included contact information. References were shown only in 14.1% of websites. The websites with higher design criteria did not have higher content points. Conclusion: The Internet remains a frequently referenced source of information. The accuracy of the information on health topics included on the Internet is important in terms of public health. Unfortunately, according to our study, the top websites giving information on influenza vaccination were not well designed and had insufficient content.
目的:互联网作为健康相关信息的来源越来越受欢迎。然而,几项研究揭示了基于网络的健康信息的质量不足。因此,本研究旨在分析流感疫苗相关网站的内容和设计。材料和方法:本研究使用土耳其语的搜索引擎Google search进行。我们确定了15个与流感相关的搜索词,对于每个词,在删除重复链接后,第一页上的前十个链接被包括在研究中。然后,我们根据内容和设计标准评估确定的网站。结果:我们的研究评估了71个与流感疫苗接种相关的环节。所有入选网站的内容标准平均得分为11.5分。学术网站的内容得分最高,为22.5分,而新闻网站和医院网站的内容得分分别为10.5分和8.9分,低于平均水平。38%的网站具有美学/内容专业性,77.5%的网站包含联系信息。只有14.1%的网站显示了参考文献。设计标准较高的网站并没有较高的内容点。结论:互联网仍然是一个经常被引用的信息来源。就公共卫生而言,互联网上关于卫生主题的信息的准确性很重要。不幸的是,根据我们的研究,提供流感疫苗信息的顶级网站设计不佳,内容不足。
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引用次数: 0
Refik Saydam: A Pioneer in the Fight Against Infectious Diseases Refik Saydam:对抗传染病的先驱
Pub Date : 2021-12-30 DOI: 10.36519/idcm.2021.109
Cemaliye Başaran
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引用次数: 0
Antifungal susceptibility profile of Candida spp. causing candidemia in an Indonesian tertiary hospital 印度尼西亚一家三级医院念珠菌引起念珠菌病的抗真菌敏感性分析
Pub Date : 2021-12-21 DOI: 10.51559/jcmid.v1i2.5
A. Wulandari, Rebriarina Hapsari, Mujahidah, Desvita Sari, Iva Puspitasari, D. Pramukarso
Introduction: Invasive candidiasis has emerged as a significant public health problem in recent years, and candidemia is the most common form. Identification of the Candida species is important since some Candida species showed an increasing trend of resistance to commonly used antifungal agents. This study was conducted to describe primary demographic data and the distribution of Candida species along with their antifungal susceptibility profiles among patients with candidemia.Methods: We conducted a retrospective descriptive study on patients with candidemia and their Candida species identification and antifungal susceptibility testing (ID&AST) results at Dr. Kariadi Hospital, Indonesia, from January 2017 to December 2020. Identification and antifungal susceptibility testing of Candida species during these 4 years were determined using the Vitek-2 system. After collecting the data, it was analyzed by using SPSS 25.sav. The data was analyzed distributively, and was presented by table and barchart.Result: A total of 85 Candida species were isolated from 74 patients. Infant age (58.10%) and intensive care (64.86%) patients contributed to most of the candidemia cases. The three most common Candida species isolated during the study period were C. parapsilosis (36.9%), C. tropicalis (25%), and C. albicans (25%). Among all Candida isolates, 98.9% were susceptible to Fluconazole and Voriconazole, 99% were susceptible to Caspofungin, Micafungin, and Flucytosine, 95.7 % were susceptible to Amphotericin B. Echinocandin resistance emerged in 2020 from a C. glabrata isolate.Conclusion: The present study, conducted in a tertiary care Indonesian hospital, showed the predominance of non-albicans Candida with favorable antifungal susceptibility profiles.
近年来,侵袭性念珠菌病已成为一个重大的公共卫生问题,而念珠菌是最常见的形式。念珠菌种类的鉴定是重要的,因为一些念珠菌种类对常用抗真菌药物的耐药性呈上升趋势。本研究旨在描述念珠菌患者的主要人口统计数据和念珠菌种类分布及其抗真菌敏感性特征。方法:对2017年1月至2020年12月印度尼西亚Dr. Kariadi医院的念珠菌感染患者及其念珠菌种类鉴定和抗真菌药敏试验(ID&AST)结果进行回顾性描述性研究。采用Vitek-2系统对4年假丝酵母菌种进行鉴定和药敏试验。收集数据后,采用SPSS 25.sav软件进行分析。数据采用分布分析,以表格和柱状图的形式呈现。结果:74例患者共分离到念珠菌85种。婴儿(58.10%)和重症监护(64.86%)患者是念珠菌感染的主要来源。在研究期间分离到的3种最常见的念珠菌是假丝酵母菌(36.9%)、热带假丝酵母菌(25%)和白色假丝酵母菌(25%)。念珠菌对氟康唑和伏立康唑的敏感性为98.9%,对卡泊芬净、米卡芬净和氟胞嘧啶的敏感性为99%,对两性霉素b的敏感性为95.7%。结论:目前的研究,在三级护理印度尼西亚医院进行,显示非白色念珠菌具有良好的抗真菌敏感性的优势。
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引用次数: 1
A rare finding of Burkholderia pseudomallei isolate from neck abscess, 4 years after the last report in Makassar: a case report 在望加锡最后一次报告4年后,从颈部脓肿中发现罕见的假马利氏伯克霍尔德菌分离物:一例报告
Pub Date : 2021-12-21 DOI: 10.51559/jcmid.v1i2.8
Andi Meutiah Ilhamjaya, Munawir Muhammad, Ahmad Rahmat Ramadhan, R. Sjahril
Introduction: Burkholderia pseudomallei is the causative agent of melioidosis, known as Whitmore's disease, a tropical disease in humans. The bacteria can infect humans or animals, mostly found in contaminated water and soil. It spreads by direct contact with contaminated sources, inhalation or ingestion of contaminated dust or water droplets, or contact with soil through skin abrasions. Melioidosis is highly endemic in Southeast Asia and Northern Australia but rare in Makassar.Case description: A 44-year-old woman shows a red mass on the right neck, reddish, palpable, ±6x5x4mm in size, and is soft in consistency and has a cystic impression. Direct gram showed purulent mixed with blood. 24 hours after incubation under aerobic conditions, grew small colonies, smooth, cream in color, non-lactose fermenter on Mac Conkey agar. Indirect gram shows a small, gram-negative rod (safety pins appearance). The colonies were positive in the catalase and oxidase test, with citrate and urease test was negative. Burkholderia pseudomallei was identified by Vitek2 with 97% Probability. It was susceptible to doxycycline, ceftazidime, and Trimethoprim/Sulfamethoxazole.Conclusion: This is a rare finding in a clinical isolate found in Makassar. A newly emerged case, four years after the last report in Makassar. This is still a challenge to public health problems in Makassar, must be reported consistently and never lost to follow up to get proper treatment.
简介:假假杆菌伯克霍尔德氏菌是类鼻疽病的病原体,被称为惠特莫尔病,是一种人类热带疾病。这种细菌可以感染人类或动物,主要存在于被污染的水和土壤中。它通过直接接触污染源,吸入或摄入受污染的灰尘或水滴,或通过皮肤磨损与土壤接触而传播。类鼻疽病在东南亚和澳大利亚北部高度流行,但在望加锡很少见。病例描述:44岁女性,右颈部有红色肿块,微红色,可触及,大小为±6x5x4mm,质地柔软,有囊性影。直接革兰氏图显示脓性混血。在有氧条件下培养24小时后,在Mac Conkey琼脂上培养出小菌落,光滑,奶油色,无乳糖发酵。间接革兰氏示小的革兰氏阴性棒(安全别针外观)。菌落过氧化氢酶和氧化酶试验呈阳性,柠檬酸盐和脲酶试验呈阴性。用Vitek2鉴定假马尔杆菌的概率为97%。对强力霉素、头孢他啶、甲氧苄啶/磺胺甲恶唑敏感。结论:这是在望加锡发现的临床分离株中罕见的发现。这是在望加锡最后一次报告四年后新出现的病例。这仍然是望加锡公共卫生问题面临的挑战,必须持续报告,绝不能失去后续行动以获得适当治疗。
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引用次数: 0
Effect of Tocilizumab Therapy On Patient Outcome In Severe COVID-19 Pneumonia 托珠单抗治疗对重症COVID-19肺炎患者预后的影响
Pub Date : 2021-08-31 DOI: 10.36519/idcm.2021.51
M. Yıldız, F. Sultanova, H. S. Özger, Merve Büyükkörük, K. Erbay, Ü. Gaygısız, A. Tufan, Ö. Gülbahar, Fatma Zehra Özcan, Nazlı Elif Kuscu, Deniz Gazioglu, Beyza Çiftçi, F. Sen, E. Senol, Kenan Hızal, Özlem Güzel Tunçcan, Pınar Aysert Yıldız, Yeşim Yıldız, M. Dizbay
Background: This study aimed to evaluate the impact of tocilizumab treatment on clinical improvement in severe COVID-19 cases and the factors affecting clinical response
背景:本研究旨在评价托珠单抗治疗对COVID-19重症患者临床改善的影响及影响临床反应的因素
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引用次数: 0
The effects of polypharmacy, nutritional and functional status on the progression of COVID-19 in older adults 多种药物、营养和功能状况对老年人COVID-19进展的影响
Pub Date : 2021-08-31 DOI: 10.36519/idcm.2021.60
Asiye Bahar Kaçmaz, P. Soysal, S. G. Tan, B. Durdu, Y. Akkoyunlu, Hamza Ogün, Fatmanur Karaköse-Okyaltırık, Sibel Bölükcü, G. Okay, Bilge Sümbül, Bedia Gülen, Ertan Sönmez, M. Meriç-Koç, R. Kazancioglu
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引用次数: 0
COVID-19 Vaccination in The Wake of a Fourth Wave of the Pandemic: An Evidence-Based Strategy is Desperately Needed 第四波大流行过后的COVID-19疫苗接种:迫切需要循证战略
Pub Date : 2021-08-31 DOI: 10.36519/idcm.2021.82
M. Akova
As of August 21, 2021, more than 32% of the world population has received at least one dose of the COVID-19 vaccine and almost one fourth is fully vaccinated. However, only 1.4% of the population in the developing world have received at least one dose (1). While the pandemic ravages the unvaccinated communities, a new wave of the disease is emerging in countries with high rates of full vaccination status (2, 3). There may be at least three factors responsible for the latter situation: 1. Emerging new variants (e.g. delta) which are much higher contagious than their predecessors and more resistant to antibodies produced by both natural disease and the available vaccines 2. Relaxing the preventive measures (e.g. use of masks, public gatherings) in the community mostly due to initial confidence provided by vaccination 3. Waning immunity from the initial course of vaccination
截至2021年8月21日,超过32%的世界人口至少接种了一剂COVID-19疫苗,近四分之一的人接种了完全疫苗。然而,在发展中国家,只有1.4%的人口接种了至少一剂疫苗(1)。当大流行肆虐未接种疫苗的社区时,新一波疾病正在全面疫苗接种率高的国家出现(2,3)。造成后一种情况可能至少有三个因素:1。正在出现的新变种(如德尔塔病毒)比其前身具有更高的传染性,并且对自然疾病和现有疫苗产生的抗体更具抵抗力2。在社区放松预防措施(例如使用口罩、公共集会),主要是由于疫苗接种提供了初步信心3。最初接种疫苗后免疫力下降
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引用次数: 3
Diabetic Foot Infections and Outcomes: A Single Center Study 糖尿病足部感染和结果:一项单中心研究
Pub Date : 2021-08-31 DOI: 10.36519/idcm.2021.62
D. Yapar, Özlem Akdoğan, Murat Kendirci, Aysel Kocagül Çelikbaş, N. Baykam
{"title":"Diabetic Foot Infections and Outcomes: A Single Center Study","authors":"D. Yapar, Özlem Akdoğan, Murat Kendirci, Aysel Kocagül Çelikbaş, N. Baykam","doi":"10.36519/idcm.2021.62","DOIUrl":"https://doi.org/10.36519/idcm.2021.62","url":null,"abstract":"","PeriodicalId":11964,"journal":{"name":"European Journal of Clinical Microbiology and Infectious Diseases","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90970321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Predictors of COVID-19 among Healthcare Workers who were Exposed to COVID-19 暴露于COVID-19的医护人员中COVID-19的预测因素
Pub Date : 2021-08-31 DOI: 10.36519/idcm.2021.74
Ç. Erol, Tugba Yanik-Yalcin, N. Sarı, Özlem Azap Kurt, H. Arslan
,
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引用次数: 1
期刊
European Journal of Clinical Microbiology and Infectious Diseases
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