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Healthcare Workers Should be Inoculated with The Highest Effective Vaccine Available 卫生保健工作者应接种可获得的最有效疫苗
Pub Date : 2021-08-31 DOI: 10.36519/idcm.2021.47
Özlem Kurt Azap, H. Arslan, Ç. Erol, Tuğba Yanık Yalçın, N. Sarı
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引用次数: 2
Two cases of COVID-19 reinfection in Turkey 土耳其出现两例COVID-19再感染病例
Pub Date : 2021-08-31 DOI: 10.36519/idcm.2021.59
İrem Akdemir-Kalkan1, Nejat Siraj Mohammed, G. Cınar, Ezgi Gülten, E. M. Saricaoğlu, Zeynep Ceren Kip-Karahan, A. M. Bozday, A. Azap
It is not clear whether re-infection by SARS-CoV-2 occurs as a rare event or not, but since the start of the pandemic limited number of re-infection has been reported. This study reports the clinical presentations and comperative genome analysis of two patients who were suspected of re-infection. The patients showed mild clinical symptoms and varied antibody test results in the two episodes of infections. New generation sequencing of the isolates revealed the presence of different mutations between isolates from the previous and last infections. Therefore it is important to monitor these mutations and assess their capacity of evading previously occurred antibodies
目前尚不清楚SARS-CoV-2再次感染是否罕见,但自大流行开始以来,已报告了有限数量的再次感染。本研究报告了两例疑似再感染患者的临床表现和比较基因组分析。两期感染患者临床症状轻,抗体检测结果差异大。分离株的新一代测序显示,前一次和最后一次感染的分离株之间存在不同的突变。因此,监测这些突变并评估它们逃避先前发生的抗体的能力是重要的
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引用次数: 0
First Report of SARS-CoV-2 Gamma Variant in Turkey 土耳其首次报告SARS-CoV-2伽玛变体
Pub Date : 2021-08-31 DOI: 10.36519/idcm.2021.43
M. Kuşkucu, Yeşim Tuyji Tok, O. Nohut, Zarifa Abullayeva, Ebru Yücebag, F. Can, S. ŞİMŞEK YAVUZ, H. Eraksoy, K. Midilli
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引用次数: 2
The current trend for prosthetic joint infection diagnosis from culture to molecular: a literature review 人工关节感染诊断从培养到分子的发展趋势:文献综述
Pub Date : 2021-07-02 DOI: 10.51559/jcmid.v1i1.6
Pathogen identification in prosthetic joint infection is necessary to achieve optimal patient management. The specimens for diagnosis of prosthetic joint infection could be the synovial fluid, the tissue obtained intraoperatively, and the biofilm from the implanted prosthesis. Because of the low sensitivity of the conventional specimen culture method, the preanalytic treatment of the specimen was widely studied to increase the yield of detection. This review aimed to describe the current specimen processing methods used in the clinical setting to increase the pathogen detection rate. A blood culture bottle, tissue homogenization, and explanted prosthesis sonication were the most studied methods with a good result. Molecular methods were also developed to reduce the time of pathogen detection. MALDI-TOF was studied to reduce identification time after a positive culture. Other molecular methods such as polymerase chain reaction and next-generation sequencing were studied to omit the culture step and reduce detection time. However, the impracticality and the inconsistent sensitivity of certain specimens from the molecular methods limit its application in the clinical setting. Specimen culture remains as a crucial step in the current prosthetic joint infection, with the improvement of the molecular methods toward a better prosthetic joint infection diagnosis. 
假体关节感染的病原体鉴定是实现最佳患者管理的必要条件。诊断假体关节感染的标本可以是滑液、术中获得的组织和植入假体的生物膜。由于传统的标本培养方法灵敏度低,为了提高检测收率,对标本进行前分析处理得到了广泛的研究。本文综述了目前临床中用于提高病原体检出率的标本处理方法。血培养瓶法、组织匀浆法和人工体超声法是目前研究最多的方法,效果良好。分子方法的发展也减少了病原体的检测时间。研究MALDI-TOF以减少阳性培养后的识别时间。研究了其他分子方法,如聚合酶链反应和下一代测序,以省略培养步骤,缩短检测时间。然而,分子方法的不实用性和某些标本的敏感性不一致限制了其在临床环境中的应用。标本培养仍然是当前假肢关节感染的关键步骤,随着分子方法的改进,假肢关节感染的诊断也在不断完善。
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引用次数: 0
Antiviral mechanisms targeting regulatory genes Tat and Rev to defeat latent HIV-1 infected T cells: a literature review 靶向调控基因Tat和Rev的抗病毒机制打败潜伏HIV-1感染的T细胞:文献综述
Pub Date : 2021-06-28 DOI: 10.51559/jcmid.v1i1.4
E. Sintya, Ni Luh Putu Harta Wedari, I. Pranata, Ni Nyoman Sri Budayanti
Antiviral drug therapies have been utilized to prevent disease progression in patients positive HIV-1. Various research has been conducted to investigate and develop a potential functional therapy to suppress HIV-1 replication and cure latent HIV-1 in the absence of drugs. Approaches that have been well studied are the anti-HIV-1 which targets RNAs, proteins, or peptides expressed by HIV-1 resistant cells, which can be transplanted to the patients. RNA interference in the form of small RNA has been proven as a promising therapy to prevent HIV-1 replication. It is utilized for therapy using cell transplantation and various gene combinations in clinical trials. However, many studies have been failed to show a successful eradication of latently HIV-1 infected T cells. It is happened due to the virus's ability to escape from antiviral therapies. However, this can be overcome by using a combination of ARTs. On the other hand, genetic editing has been intensively studied since it can cure various diseases caused by genetic or pathogen infections, including HIV type 1. The previous studies have designed gRNA bind to protein Cas type 9 targeting HIV functional genes, Tat and Rev sequences. Various recombination has been introduced to CRISPR-based gene editing to increase the binding affinity and efficiency of Cas9 to target Tat and Rev proteins of their exons. The best approach for the Cas9 targeted Tat and Rev is by utilizing more than one guide RNA. However, Subsequent studies are needed to confirm the ability of Cas9 with various guide RNAs to inhibit virus activation and replication in latent HIV-1. This review aims to describe the mechanisms, advantages, and disadvantages of antiviral therapies that target Tat and Rev as regulatory genes to eradicate latent HIV-1 infected T cells. 
抗病毒药物治疗已被用于预防HIV-1阳性患者的疾病进展。在没有药物的情况下,已经进行了各种各样的研究来研究和开发一种潜在的功能疗法来抑制HIV-1的复制和治愈潜伏的HIV-1。目前研究比较充分的方法是靶向HIV-1抗性细胞表达的rna、蛋白质或肽的抗HIV-1药物,这些药物可以移植到患者体内。小RNA形式的RNA干扰已被证明是防止HIV-1复制的一种有希望的治疗方法。它在临床试验中用于细胞移植和各种基因组合的治疗。然而,许多研究未能显示成功根除潜伏的HIV-1感染的T细胞。这是因为病毒有能力逃避抗病毒治疗。然而,这可以通过联合使用抗逆转录病毒药物来克服。另一方面,基因编辑可以治愈包括HIV 1型在内的多种由遗传或病原体感染引起的疾病,因此受到了广泛的研究。先前的研究设计了gRNA结合到靶向HIV功能基因Tat和Rev序列的cas9蛋白上。基于crispr的基因编辑中引入了各种重组,以提高Cas9对Tat和Rev蛋白外显子的结合亲和力和效率。针对Cas9靶向Tat和Rev的最佳方法是利用多个引导RNA。然而,需要后续的研究来证实Cas9与各种引导rna一起抑制潜伏HIV-1病毒激活和复制的能力。这篇综述旨在描述以Tat和Rev为调控基因的抗病毒治疗的机制、优缺点,以根除潜伏的HIV-1感染的T细胞。
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引用次数: 2
Urinary Tract Infection (UTI) caused by Extended-Spectrum Beta-Lactamase (ESBL)-producing Enterobacteriaceae: a case series 广谱β -内酰胺酶(ESBL)产生肠杆菌科引起的尿路感染(UTI):一个病例系列
Pub Date : 2021-06-28 DOI: 10.51559/jcmid.v1i1.2
Qonita Imma Irfani, Dessy Kurnia Sari, Dewi Purbaningsih, T. Wibawa, T. Nuryastuti
Introduction: A urinary tract infection (UTI) is an infection in any part of the urinary system, occurring in the kidneys, bladder, ureters, and urethra. UTI incidence in Indonesia was high enough. The prevalence of UTI in the community enhances in line with the increase of age. 40-60 years had a prevalence rate of 3.2%, while at the age of  ≥65, a UTI had a prevalence rate of 20%. UTIs are among the most common infections in humans. Enterobacteria that produce extended-spectrum beta-lactamase (ESBL) is one of the most frequent causes. Prevalence of the ESBL bacterial infection is developing due to the widespread prescription of antibiotics around the world. Thus, our articles established several UTI cases of various ages and their antibiotic susceptibility.Case Description: This case report series presents eight cases describing a UTI caused by Enterobacteriaceae producing ESBL in various ages. Data were collected retrospectively from secondary sources of laboratory results. Patient baby-child mostly had a history of sepsis. Other than that patient had hydronephrosis kidney, seizure, fever. On the other hand, the patient's old man and woman had a history of fever, shock spinal and fracture, malignancy. All patients were tested for antibiotic susceptibility and mostly still sensitive with amikacin, meropenem, nitrofurantoin, and fosfomycin.Conclusions: Our case series established that patients with UTI were mainly still sensitive to amikacin, meropenem, nitrofurantoin, and fosfomycin. We should implement and maintain contact precautions throughout the hospital wards to prevent the transmission of ESBL infections.
导读:尿路感染(UTI)是泌尿系统任何部位的感染,发生在肾脏、膀胱、输尿管和尿道。印度尼西亚的尿路感染发病率足够高。社区尿路感染的患病率随着年龄的增长而增加。40-60岁的尿路感染患病率为3.2%,≥65岁的尿路感染患病率为20%。尿路感染是人类最常见的感染之一。肠杆菌产生广谱β -内酰胺酶(ESBL)是最常见的原因之一。由于抗生素在世界范围内的广泛使用,ESBL细菌感染的流行正在发展。因此,我们的文章建立了几个不同年龄的尿路感染病例及其抗生素敏感性。病例描述:本病例报告系列介绍了8例由肠杆菌科产生ESBL引起的不同年龄的尿路感染。数据是从实验室结果的二手来源回顾性收集的。患儿多有败血症史。除了那个病人有肾积水,癫痫,发烧。另一方面,患者的老年男女有发热、休克、脊柱骨折、恶性肿瘤史。所有患者均进行抗生素敏感性检测,大多数患者对阿米卡星、美罗培南、呋喃妥因和磷霉素仍敏感。结论:我们的病例系列表明,尿路感染患者主要仍对阿米卡星、美罗培南、呋喃妥因和磷霉素敏感。我们应该在整个医院病房实施和保持接触预防措施,以防止ESBL感染的传播。
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引用次数: 0
A six-month epidemiologic study of carbapenem-resistant Acinetobacter baumannii and carbapenem-resistant Pseudomonas aeruginosa in early pandemic at Dr. Soetomo Hospital Surabaya 泗水Soetomo医生医院大流行早期耐碳青霉烯鲍曼不动杆菌和耐碳青霉烯铜绿假单胞菌六个月流行病学研究
Pub Date : 2021-06-16 DOI: 10.51559/jcmid.v1i1.1
Pristiawan Navy Endraputra, Kuntaman, Eko Budi Khoendori
Background: Nosocomial infection is derived from the patient itself or transmitted across patients, healthcare workers, or the hospital environment. Carbapenem-resistant Acinetobacter baumannii (CRAB) and Pseudomonas aeruginosa (CRPA) have been recognized as the most common pathogen for nosocomial infections in Southeast Asia. Afterward, CRAB and CRPA infections increase morbidity, mortality, length of stay, and cost. This study examined the prevalence of CRAB and CRPA at Dr. Soetomo Hospital, Surabaya, from January to June 2020.Methods: This descriptive study was conducted retrospectively by collecting data from the first isolate per specimen per patient of Acinetobacter baumannii and Pseudomonas aeruginosa, resistant either to meropenem or imipenem from urine, blood, pus, or sputum in Dr. Soetomo Hospital, Surabaya within January to June 2020.Results: A total of 311 Acinetobacter baumannii and 425 Pseudomonas aeruginosa were collected with 134 CRAB and 79 CRPA. The resistance rate of CRAB was ≥90% except for amikacin, cefoperazone-sulbactam, levofloxacin, tigecycline, trimethoprim-sulfamethoxazole, and fosfomycin. Moreover, CRPA had resistance ≥90% regardless of intrinsic resistance against imipenem and colistin. There was an increase in CRAB and CRPA, with the highest incidence in June 2020 in early 2020.Conclusion: The prevalence of CRAB and CRPA were 43% and 19%, respectively, in the early six months of 2020 at Dr. Soetomo Hospital, Surabaya.
背景:医院感染来源于患者自身或在患者、医护人员或医院环境中传播。耐碳青霉烯鲍曼不动杆菌(CRAB)和铜绿假单胞菌(CRPA)是东南亚地区最常见的医院感染病原体。之后,CRAB和CRPA感染会增加发病率、死亡率、住院时间和费用。本研究调查了2020年1月至6月泗水Soetomo医生医院的CRAB和CRPA患病率。方法:本描述性研究回顾性收集了2020年1月至6月泗水Dr. Soetomo医院每位患者的尿液、血液、脓液或痰中对美罗培南或亚胺培南耐药的鲍曼不动杆菌和铜绿假单胞菌的首个样本分离物的数据。结果:共检出鲍曼不动杆菌311株,铜绿假单胞菌425株,其中CRAB 134株,CRPA 79株。除阿米卡星、头孢哌酮-舒巴坦、左氧氟沙星、替加环素、甲氧苄啶-磺胺甲恶唑、磷霉素外,其他5种药物的耐药率均≥90%。此外,无论对亚胺培南和粘菌素的内在耐药性如何,CRPA的耐药率均≥90%。螃蟹和CRPA呈上升趋势,2020年初以2020年6月发病率最高。结论:泗水Dr. Soetomo医院2020年上半年的CRAB和CRPA患病率分别为43%和19%。
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引用次数: 0
Current recombinant vaccine strategy as a novel approach to prevent Ebola virus diseases: a literature review 当前重组疫苗策略作为预防埃博拉病毒病的新途径:文献综述
Pub Date : 2021-06-16 DOI: 10.51559/jcmid.v1i1.3
Agus Simahendra, Ni Luh Putu Harta Wedari, I. Pranata, Ni Nyoman Sri Budayanti
Ebola virus has resulted in a devastating hemorrhagic fever epidemic spanning several African countries and leading to thousands of deaths. There have been no vaccines approved or medication strategies toward successful prophylaxis and therapeutics critical until the rVSV-ZEBOV vaccine approved by the US Food and Drug Administration (FDA) in December 2019 as a preventative measure for people aged 18 years old and/or older. Several experimental vaccines are showing some promise. The most advanced vaccine is the clinically tested recombinant vesicular-stomatitis virus (rVSV) which encodes EBOV glycoprotein, widely known as the V920 vaccine candidate. This vaccine induces antibody-producing responses in non-human primate models, and current clinical trials suggest protective efficacy in humans. Although generally well-tolerated, the administration of this vaccine was complicated by occurrences of side effects. The development of vaccine platforms is also challenging, given that Ebola virus diseases have now reached epidemic proportions in some localities. Outcomes in terms of viral persistence after recovery are unknown, and a study explaining the role of adaptive immunity in recovery may be essential to inform effective vaccine design. This review aims to give a basic understanding on the general immunity mechanism elicited by recombinant vector vaccines and the current implementation of this relatively new technology to tackle a major infectious disease outbreak.
埃博拉病毒在几个非洲国家引发了毁灭性的出血热疫情,导致数千人死亡。在2019年12月美国食品和药物管理局(FDA)批准rVSV-ZEBOV疫苗作为18岁和/或以上人群的预防措施之前,还没有批准的疫苗或成功预防和治疗的药物策略。几种实验性疫苗显示出一些希望。最先进的疫苗是经临床试验的重组水泡性口炎病毒(rVSV),它编码EBOV糖蛋白,被广泛称为V920候选疫苗。该疫苗在非人类灵长类动物模型中诱导产生抗体反应,目前的临床试验表明对人类有保护作用。虽然这种疫苗的耐受性一般良好,但由于副作用的发生,使用这种疫苗变得复杂。鉴于埃博拉病毒疾病现已在一些地区达到流行病的程度,疫苗平台的开发也具有挑战性。康复后病毒持续存在的结果尚不清楚,一项解释适应性免疫在康复中的作用的研究可能对有效的疫苗设计至关重要。本综述旨在对重组载体疫苗引发的一般免疫机制以及目前这一相对较新的技术在应对重大传染病暴发中的应用进行基本了解。
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引用次数: 0
Persistent Symptoms After Discharge of COVID-19 Patients COVID-19患者出院后持续症状
Pub Date : 2021-04-30 DOI: 10.36519/idcm.2021.40
O. Evlice, F. Kuş, M. Bektaş
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引用次数: 3
2021: The Year of Health Care Workers 2021年:卫生保健工作者年
Pub Date : 2021-04-30 DOI: 10.36519/idcm.2021.36
Özlem Azap Kurt
{"title":"2021: The Year of Health Care Workers","authors":"Özlem Azap Kurt","doi":"10.36519/idcm.2021.36","DOIUrl":"https://doi.org/10.36519/idcm.2021.36","url":null,"abstract":"","PeriodicalId":11964,"journal":{"name":"European Journal of Clinical Microbiology and Infectious Diseases","volume":"311 1","pages":"45-47"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91310633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
European Journal of Clinical Microbiology and Infectious Diseases
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