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Multiple Nodular Lesions in Spleen and Liver in Visceral Leishmaniasis 内脏利什曼病脾、肝多发结节性病变
Pub Date : 2019-10-07 DOI: 10.36519/idcm.2019.19011
S. Kutlu, M. Kutlu, D. Herek, D. Erdoğan, Kevser Ozdemi̇r, Nilay Sen Turk, Olga Yaylalı, S. Hacıoğlu, H. Turgut
1 Department of Infectious Diseases and Clinical Microbiology, Pamukkale University School of Medicine, Denizli, Turkey 2 Department of Radiology, Pamukkale University School of Medicine, Denizli, Turkey 3 Department of Parasitology, Ege University School of Medicine, İzmir, Turkey 4 Department of Pathology, Pamukkale University School of Medicine, Denizli, Turkey 5 Department of Nuclear Medicine, Pamukkale University School of Medicine, Denizli, Turkey 6 Department of Hematology, Pamukkale University School of Medicine, Denizli, Turkey
1土耳其代尼兹利Pamukkale大学医学院传染病与临床微生物学系2土耳其代尼兹利Pamukkale大学医学院放射学学系3土耳其埃格大学医学院寄生虫学学系İzmir 4土耳其代尼兹利Pamukkale大学医学院病理学系5土耳其代尼兹利Pamukkale大学医学院核医学系6血液学学系Pamukkale大学医学院,代尼兹利,土耳其
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引用次数: 2
The Factors Affecting Specialty Preference and Job Satisfaction of Medical Residents 医疗住院医师专业偏好与工作满意度的影响因素
Pub Date : 2019-06-12 DOI: 10.5152/IDCM.2019.19006
Yusuf Kuzukiran, Busra Kurtul, Betul Kurtul, Zehra Tarhan, Oğuzhan Taş, A. Azap
Objective: The choice of a residency program is one milestone in the professional life of a medical doctor. Residents may be affected by many factors while choosing a specialty, and if some of these factors come true during the residency period, job satisfaction (JS) can be high. We aimed to improve our understanding of the factors affecting the selection of specialties by medical doctors, JS of residents, and factors affecting the JS. Materials and Methods: The study was conducted in a university hospital with 932 beds and 240 residents. Data were collected by a questionnaire consisting of 39 questions to achieve information about demographic characteristics and the factors affecting the preferences of the residents. Participants were also asked to answer the Minnesota Job Satisfaction Questionnaire to obtain their JS scores. Results: JS of the residents was high in 69.7% (n=108) of participants. Residents who have chosen their current specialty from within the first three options according to their rank in specialty exam had higher JS (p=0.023). The top factors affecting the residents’ preference were an inclination towards the specialty and compliance with personal characteristics. The most crucial factor for choosing a surgical specialty was “an inclination towards the specialty” while “compliance with personal characteristics” was the most crucial factor for choosing non-surgical specialties. The factors that affect Infectious Diseases and Clinical Microbiology (IDCM) residents’ preference were the quality of the education, the inclination for the specialty, compliance with personal characteristics and career opportunities. JS of IDCM residents was very high (%100). For all residents, the inclination for the specialty was more critical than expected economic income (p<0.001). Conclusion: Inclination towards the specialty is the primary determinant for the preference of specialty among medical doctors. The educational quality of the residency program and career opportunities are also important.
目的:住院医师项目的选择是医生职业生涯中的一个里程碑。住院医师在选择专业时可能会受到许多因素的影响,如果其中一些因素在住院期间得以实现,那么工作满意度(JS)就会很高。我们的目的是提高我们对医生选择专科的影响因素、住院医师的JS以及影响JS的因素的了解。材料与方法:本研究在某大学附属医院进行,该医院共有床位932张,住院医师240人。通过问卷调查收集数据,问卷共39个问题,了解人口特征和影响居民偏好的因素。参与者还被要求回答明尼苏达州工作满意度问卷,以获得他们的JS分数。结果:69.7% (n=108)的居民JS较高。根据专业考试排名在前三个选项中选择当前专业的住院医师JS较高(p=0.023)。影响住院医师选择的最主要因素是专业倾向和符合个人特点。选择外科专业最关键的因素是“对专业的倾向性”,而选择非手术专业最关键的因素是“符合个人特点”。影响传染病与临床微生物学(IDCM)住院医师专业偏好的因素为教育质量、专业倾向、符合个人特点和就业机会。IDCM居民的JS非常高(%100)。对于所有居民来说,专业倾向比预期经济收入更重要(p<0.001)。结论:专科倾向性是医师专业偏好的主要决定因素。住院医师项目的教育质量和就业机会也很重要。
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引用次数: 0
Safety of a Carbapenem-Sparing Approach as Part of an Antibiotic Stewardship Program, in a Setting with Increased Carbapenem Resistance 在碳青霉烯耐药性增加的情况下,作为抗生素管理计划一部分的碳青霉烯节约方法的安全性
Pub Date : 2019-06-12 DOI: 10.5152/IDCM.2019.19003
A. Makina, G. Poulakou, S. Sympardi, M. Souli, E. Liakopoulou, D. Matthaiou, Aggeliki Karaiskou, A. Arvaniti, Nikoleta Charalabaki, A. Antoniadou, A. Papadopoulos, Eleftheria Trikka-Grafakos, A. Sklavounos, H. Giamarellou
1 Antimicrobial Stewarship Program Team, Thriasio General Hospital of Elefsina, Attica, Greece; 2 3rd Department of Medicine, National and Kapodistrian University of Athens, Medical School, Sotiria General Hospital of Athens, Greece; 3 4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, Attikon University General Hospital of Athens, Greece; 4 Department of Critical Care, Attikon University General Hospital of Athens, Greece; 5 Department of Intensive Care, Viokliniki Athinon, Athens, Greece; 6 1st Department of Internal Medicine-Infectious Diseases, Hygeia General Hospital, Athens, Greece
1希腊阿提卡埃莱夫西纳特里拉西奥总医院抗菌蒸汽项目小组;2希腊雅典国立和卡波迪斯特里安大学医学院第三医学部,雅典Sotiria总医院;3希腊雅典国立大学和卡波迪斯特里安大学医学院内科第4科;4希腊雅典阿提肯大学总医院重症监护科;5希腊雅典Viokliniki Athinon重症监护室;6希腊雅典Hygeia总医院内科-感染性疾病1科
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引用次数: 2
Biofilm Formation of Acinetobacter baumannii Under in vitro and in vivo Colistin Exposure 体外和体内暴露于粘菌素下鲍曼不动杆菌生物膜的形成
Pub Date : 2019-06-12 DOI: 10.5152/IDCM.2019.19004
Berna Özer, Cansel Vatansever, O. Dogan, Ş. Keske, O. Ergonul, F. Can
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引用次数: 0
Rhombencephalitis with Listeria monocytogenes 菱形脑炎伴单核细胞增生李斯特菌
Pub Date : 2019-06-12 DOI: 10.5152/IDCM.2019.19008
A. Yerlikaya, B. Eryılmaz, B. Kahyaoglu, O. Ergonul
We described clinical course and outcome of a patient with Listeria monocytogenes meningitis and rhombencephalitis (RE). A 65-year-old woman with past medical history of chemotherapy and radiotherapy for cervical cancer applied to emergency room with the history of fever and headache for three days. Clinical course indicating meningitis evolved to RE over time. We present this case because of the significance of early diagnosis and adequate antimicrobial therapy targeted to the pathogen. At the end, we suggested an algorithm for the case management.
我们描述了一例单核细胞增生李斯特菌脑膜炎和菱形脑炎(RE)患者的临床过程和结果。65岁女性,既往宫颈癌化疗、放疗病史,以发热、头痛3天就诊于急诊室。临床病程表明脑膜炎随着时间的推移演变为RE。我们提出这一情况,因为早期诊断和适当的抗菌治疗针对病原体的意义。最后,提出了一种病例管理算法。
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引用次数: 2
The effectiveness of the Culture for Dientamoeba fragilis from the Stool 粪便中脆弱地阿米巴培养的效果
Pub Date : 2019-06-12 DOI: 10.5152/IDCM.2019.19005
Ö. Kurt, N. Girginkardesler, A. Özbilgin, Ü. Z. Ok
Objective: In the identification of Dientamobea fragilis, Polymerase chain reaction (PCR) is by far more sensitive than microscopic examination of permanent-stained smears, while it is faster and less labour-intensive than culture; however, it is expensive. We aimed to assess the sensitivity of culture and compare the efficacies of three culture media: Robinson’s medium (RM), Dobell’s medium (DM) and Talis’s Medium (TM), for the identification of D. fragilis. Materials and Methods: Stool samples of 104 individuals admitted to Manisa Celal Bayar University Hospital with complaints such as abdominal pain, itching, meteorism and diarrhea, and no detection of D. fragilis in the initial examination of trichrome-stained smears of their stools, were cultivated in RM, DM and TM on the same day. Results: Trophozoites of D. fragilis were detected in 11 of 104 (10.6%) samples, where all were positive in RM, while 3 and 4 of 11 were also positive in DM and TM, respectively. Two samples were found to be positive in all three media. RM was by far the leading medium to supply vast numbers of trophozoites. Conclusion: These results indicate that culture is more effective than microscopic examination of permanent stained smears of stools to identify D. fragilis when PCR is not available, and RM could be the first choice for culture as it supplies huge amounts of trophozoites useful for further molecular studies.
目的:聚合酶链反应(PCR)在鉴定脆弱双塔莫菌时,灵敏度远高于永久染色涂片显微镜检查,而比培养更快,劳动强度更低;然而,它是昂贵的。我们的目的是评估培养的敏感性,并比较三种培养基:罗宾逊培养基(RM)、多贝尔培养基(DM)和塔利斯培养基(TM)对脆性裂裂菌的鉴定效果。材料与方法:将104例以腹痛、瘙痒、头晕、腹泻等症状就诊于马尼萨·塞拉尔·巴亚尔大学医院,粪便三色涂片初检未检出脆弱梭菌的患者,于同一天在RM、DM和TM进行培养。结果:104份样品中检出脆弱单胞菌滋养体11份(10.6%),均为RM阳性,DM阳性3份,TM阳性4份。两份样本在所有三种培养基中均呈阳性。到目前为止,RM是提供大量滋养体的主要介质。结论:这些结果表明,在没有PCR的情况下,培养比显微镜检查粪便永久染色涂片更有效地鉴定脆弱单胞菌,RM可作为培养的首选,因为它提供了大量的滋养体,可用于进一步的分子研究。
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引用次数: 1
Photo Quiz; Anthrax or Orf? 照片测验;炭疽还是口蹄疫?
Pub Date : 2019-06-12 DOI: 10.5152/IDCM.2019.19002
N. Tulek, Esra Kaya Kılıç, G. Ertem, Esra Yüksekkaya, Ankara Training Clinical Microbiology
A 38-year-old man presented to the hospital with complaints of fever and multiple lesions on his face, hands, and arms. The lesions were appeared five days earlier before admission as painless small papules and developed into larger lesions with the darkish center. He was experiencing a fever for the last 24 hours. He was a farmer, he sacrificed and butchered a bull at the Eid al-Adha, ten days ago. He reported that he was healthy previously and did not use any drug. On physical examination, his body temperature was 39°C. He had multiple lesions on arms, hands, face and erythematous diffuse edema on his right hand, on 4th finger extending to the arm. There were some satellite lesions on the left arm (Figure1, 2), as well. He had a red lymphangitic streak on the right arm that extended from the hands toward the axillae with a palpable and tender axillary lymph node 2 cm in diameter. Laboratory examination revealed white blood count 11600 cells/μL with left shift, and C-reactive protein 85 mg/ dL (N: 0-5). Aspiration material was obtained from the lesion on the 4th finger, stained with Gram stain and cultured.
一名38岁男性以发热和面部、手部和手臂多发病变主诉就诊。该病变于入院前5天出现,为无痛性小丘疹,后来发展为较大的病灶,中心呈黑色。过去24小时他一直在发烧。他是个农民,十天前,他在宰牲节上宰杀了一头公牛。他报告说,他以前很健康,没有使用任何药物。体格检查体温39℃。手臂、手部、面部多处病变,右手4指至上臂红斑性弥漫性水肿。左臂也有一些卫星病变(图1,2)。患者右臂有一红色淋巴管条纹,从手向腋窝延伸,腋窝淋巴结直径2厘米,可触及且柔软。实验室检查:白细胞11600个/μL,左移,c -反应蛋白85 mg/ dL (N: 0 ~ 5)。取4指病变部位吸出材料,革兰氏染色培养。
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引用次数: 0
Identification and Assemblage Types of Giardia duodenalis from Patients in Thrace, Turkey 土耳其色雷斯患者十二指肠贾第虫的鉴定和组合类型
Pub Date : 2019-06-11 DOI: 10.5152/IDCM.2019.19001
Sadiye Kaplan Kucuk, G. Akyildiz, R. Bircan, N. Yilmazer, A. Gargılı, S. Kar
Objective: Giardiasis is a common disease, and clinical forms can vary based on the assemblage types of the parasite. Detailed information on the subgenotypes may indicate the transmission routes and enlighten the gaps in the epidemiology of the disease. This study aims to reveal the occurrence of giardiasis in Thrace, Turkey, and assemblage types of Giardia duodenalis. Materials and Methods: In total, 573 stool samples taken from the individuals applied to Tekirdağ Central State Hospital in 2009, were examined by wet-mount and zinc sulfate floatation methods. Giardia-positive 26 samples and 64 samples taken from the individuals with gastrointestinal complaints were analyzed by nested PCR-RFLP to differentiate the assemblage types. Sequence analysis was employed for confirmation of assemblage types and subgenotypes. Results: Giardia spp. cysts were detected in 3.66% and 4.54% of the samples with wetmount and zinc sulphate floatation techniques respectively. A total of 27 samples were found positive by nested PCR-RFLP out of 90 samples. Fifteen samples were determined as assemblage A, 2 and 10 samples as B and B/E mix respectively. Sequence analysis showed that the latter assemblage (B/E mix) as A3-B3 mix. Conclusion: Fast identification techniques, namely zinc sulphate flotation can be used for screening stool samples in order to determine Giardia cysts with considerably high sensitivity and specificity. Based on this method, the occurrence rate of giardiasis was found as 4.54% in the studied group. DNA sequencing is necessary to distinguish assemblages and confirm the results of PCR-RFLP.
目的:贾第虫病是一种常见的疾病,临床形式可根据寄生虫的组合类型而变化。亚基因型的详细信息可能指示传播途径,并启发疾病流行病学的空白。本研究旨在揭示贾第虫病在色雷斯、土耳其的发生和十二指肠贾第虫的聚集类型。材料和方法:采用湿法浮法和硫酸锌浮法对2009年在泰克尔达伊尔中央国家医院采集的573份粪便样本进行检测。采用巢式PCR-RFLP方法对26份贾第鞭毛虫阳性标本和64份胃肠道疾病患者标本进行分析,以区分其组合类型。序列分析确定了组合型和亚基因型。结果:湿法和硫酸锌法检出贾第鞭毛虫囊蚴的检出率分别为3.66%和4.54%。巢式PCR-RFLP检测90份样品中27份呈阳性。15个样品分别确定为组合A, 2个样品确定为组合B, 10个样品确定为组合B/E混合物。序列分析表明,后一组合(B/E混合)为A3-B3混合。结论:快速鉴定技术即硫酸锌浮选技术可用于粪便样品筛选,对贾第鞭毛虫囊肿的检测具有较高的敏感性和特异性。根据该方法,研究组贾第虫病发病率为4.54%。DNA测序是区分组合和确认PCR-RFLP结果的必要手段。
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引用次数: 1
CRISPR-Cas System: A Brief History and Future in the Diagnosis and Treatment of Infectious Diseases CRISPR-Cas系统:传染病诊断和治疗的简史和未来
Pub Date : 2019-06-11 DOI: 10.5152/IDCM.2019.19007
B. Otlu
T he discovery of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) and CRISPR-Associated System (Cas) have led to revolutionary advancements in almost every field of biology. The CRISPR-Cas system is part of an adaptive immune system in bacteria, and this system protects the organism from invading genetic elements of bacteriophages and plasmids. In this article, the discovery of the CRISPR-Cas system, its mechanism of action, and particularly its use in infectious diseases will be discussed.
聚类规则间隔短回文重复序列(CRISPR)和CRISPR相关系统(Cas)的发现几乎在生物学的每个领域都带来了革命性的进步。CRISPR-Cas系统是细菌适应性免疫系统的一部分,该系统保护生物体免受噬菌体和质粒遗传元素的入侵。本文将讨论CRISPR-Cas系统的发现及其作用机制,特别是其在传染病中的应用。
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引用次数: 1
Streptococcus agalactiae (Group B Streptococcus) – An Uncommon Cause of Postpartum Spinal Epidural Abscess 无乳链球菌(B群链球菌)-产后脊髓硬膜外脓肿的罕见病因
Pub Date : 1900-01-01 DOI: 10.36519/idcm.2021.24
A. Cohen, Elchanan Parnasa, N. Hiller, Oren Wasser, Josh E. Schroeder, Hananel Shear-Yashuv, J. Strahilevitz
A nulliparous previously healthy pregnant woman who suffered from postpartum spinal epidural abscess due to Streptococcus agalactiae which probably evolved during delivery.
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引用次数: 0
期刊
European Journal of Clinical Microbiology and Infectious Diseases
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