Pub Date : 2023-12-08DOI: 10.15312/eurasianjvetsci.2023.411
Z. Ozdemir Kutahya, Petek PİNER BENLİ
Aim:This study aimedto investigate the effect of robenacoxibonhematological and biochemical parameters in healthy Alpine and Saanen goats 24 hours after subcutaneous administration. Materials and Methods: 12 healthy Alpine (n=6) and Saanen (n=6) breed goats were used. Robenacoxib was administered to Alpine and Saanen goats at a dose of 4 mg/kg once subcutaneously. Blood samples were collected before drug administration (0 hour) and then 24-hours. Hematological and biochemical parameters were analyzed using a hematology analyzer and biochemistry auto analyzer, respectively. Results: No change was observed in the clinical examination findings of both goat breeds. Statistical differences in hematological and biochemical parameters were determined within physiological limits in both goat breeds. The white blood cell, red blood cell and lymphocyte values were found to be low, and the monocyte% value was found to be high in Alpine goats. Decreases in red blood cell, hemoglobin and hematocrit levels and increases in the mean corpuscular volume and mean corpuscular hemoglobin values were observed at 24-hours in Saanen goats. Robenacoxib increases blood urea nitrogen value in both goat breeds, it decreases creatinine value in Saanen goats. Conclusion: There were no significant changes in hematological and biochemical parameters after robenacoxib administrations to Alpine and Saanen goats. Consequently, further studies are needed to investigate the effects of robenacoxib in high-dose and repeated administrations, liver and kidney dysfunctions, different age, breed, and physiological conditions. It was evaluated that robenacoxib could be used as an alternative NSAID to prevent pain and inflammation in goats, since no adverse effect was observed in the blood profile.
{"title":"Hematological and biochemical effect of subcutaneous administration of robenacoxib in different goat breeds","authors":"Z. Ozdemir Kutahya, Petek PİNER BENLİ","doi":"10.15312/eurasianjvetsci.2023.411","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.411","url":null,"abstract":"Aim:This study aimedto investigate the effect of robenacoxibonhematological and biochemical parameters in healthy Alpine and Saanen goats 24 hours after subcutaneous administration. Materials and Methods: 12 healthy Alpine (n=6) and Saanen (n=6) breed goats were used. Robenacoxib was administered to Alpine and Saanen goats at a dose of 4 mg/kg once subcutaneously. Blood samples were collected before drug administration (0 hour) and then 24-hours. Hematological and biochemical parameters were analyzed using a hematology analyzer and biochemistry auto analyzer, respectively. Results: No change was observed in the clinical examination findings of both goat breeds. Statistical differences in hematological and biochemical parameters were determined within physiological limits in both goat breeds. The white blood cell, red blood cell and lymphocyte values were found to be low, and the monocyte% value was found to be high in Alpine goats. Decreases in red blood cell, hemoglobin and hematocrit levels and increases in the mean corpuscular volume and mean corpuscular hemoglobin values were observed at 24-hours in Saanen goats. Robenacoxib increases blood urea nitrogen value in both goat breeds, it decreases creatinine value in Saanen goats. Conclusion: There were no significant changes in hematological and biochemical parameters after robenacoxib administrations to Alpine and Saanen goats. Consequently, further studies are needed to investigate the effects of robenacoxib in high-dose and repeated administrations, liver and kidney dysfunctions, different age, breed, and physiological conditions. It was evaluated that robenacoxib could be used as an alternative NSAID to prevent pain and inflammation in goats, since no adverse effect was observed in the blood profile.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"8 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139184830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.15312/eurasianjvetsci.2023.415
Ayşegül Bulut, Goktug Senturk, Durmus Hatipoglu, M. B. Ateş
Aim: This study aimed to investigate the protective effects of carvacrol (CRV) on rat liver against acrylamide (AA)-induced hepatotoxicity. Materials and Methods: A total of 30 Wistar Albino rats were used in the study. Rats were divided into 4 groups: Control (n:6), CRV (n:8, 50 mg/kg/ day carvacrol, i.p), AA (n:8, 40 mg/kg/day acrylamide, gavage) and AA+CRV (n:8, 40 mg/kg/day acrylamide, gavage + 50 mg/kg/day carvacrol i.p). At the end of the 15-day study period, the rats, whose blood samples were taken under anesthesia, were euthanized and necropsied. Liver function tests were examined from serums. At the same time for histological analysis, scoring, and hematoxylin-eosin staining, liver tissues were preserved in 10% formaldehyde solution. Results: In microscopic examination, it was determined that AA causes hydropic/vacuolar degeneration, fatty changes, necrosis/apoptosis, bile duct proliferation, hepatic cord dissociation, congestion, megalocytosis and mononuclear cell infiltration. Parallelly, AA raised the levels of ALP, AST, and ALT. The inclusion of CRV to the diet improved histological structure and liver function tests considerably. These results show that CRV has a hepatoprotective effect by reducing AA-induced liver damage. Conclusion: This study showed that CRV attenuated AA-induced liver injury. It is considered that its preventive effect against liver damage is due to its antioxidant properties. However, more research is needed to determine the specific mechanism at the molecular level and offer therapeutic management suggestions.
{"title":"Carvacrol alleviates acrylamide-induced hepatotoxicity in rats: An Experimental insights","authors":"Ayşegül Bulut, Goktug Senturk, Durmus Hatipoglu, M. B. Ateş","doi":"10.15312/eurasianjvetsci.2023.415","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.415","url":null,"abstract":"Aim: This study aimed to investigate the protective effects of carvacrol (CRV) on rat liver against acrylamide (AA)-induced hepatotoxicity. Materials and Methods: A total of 30 Wistar Albino rats were used in the study. Rats were divided into 4 groups: Control (n:6), CRV (n:8, 50 mg/kg/ day carvacrol, i.p), AA (n:8, 40 mg/kg/day acrylamide, gavage) and AA+CRV (n:8, 40 mg/kg/day acrylamide, gavage + 50 mg/kg/day carvacrol i.p). At the end of the 15-day study period, the rats, whose blood samples were taken under anesthesia, were euthanized and necropsied. Liver function tests were examined from serums. At the same time for histological analysis, scoring, and hematoxylin-eosin staining, liver tissues were preserved in 10% formaldehyde solution. Results: In microscopic examination, it was determined that AA causes hydropic/vacuolar degeneration, fatty changes, necrosis/apoptosis, bile duct proliferation, hepatic cord dissociation, congestion, megalocytosis and mononuclear cell infiltration. Parallelly, AA raised the levels of ALP, AST, and ALT. The inclusion of CRV to the diet improved histological structure and liver function tests considerably. These results show that CRV has a hepatoprotective effect by reducing AA-induced liver damage. Conclusion: This study showed that CRV attenuated AA-induced liver injury. It is considered that its preventive effect against liver damage is due to its antioxidant properties. However, more research is needed to determine the specific mechanism at the molecular level and offer therapeutic management suggestions.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139185160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.15312/eurasianjvetsci.2023.418
Mustafa Arican, E. Uzunlu, Yasemin Tanirli, Halil Caltiner, İremsu Satici
Progressive retinal atrophy (PRA) is described as an inherited degenerative disease of the retina that is common in dogs. The case was a 9-year-old female Turkish shepherd dog. It was presented to the Small Animal Hospital with complaints of polyuria and stranguria. After examination, the diagnosis was acute hepatitis. Medical treatment (ursofalk, milk thistle, prednisolone and N acetyl cystein) was given. The case lost vision after three days of treatment. The ophthalmological examinations were performed as included slit lamp, Schirmer's tear test, applanation tonometry, fluorescein staining and ultrasound. Ophthalmoscopic and fundoscopic examinations revealed hyperreflectivity and colour change in the tapetal region, marked attenuation of retinal vessels, depigmentation in the non-tapetal region, and optic disc atrophy with scalloped borders. Fundoscopic examination revealed retinal degeneration in both eyes. The aim of this case report is to diagnose PRA in Turkish shepherd dogs, to highlight its significance and to share the results with clinicians.
进行性视网膜萎缩（PRA）是一种遗传性视网膜变性疾病，常见于犬类。该病例是一只 9 岁的雌性土耳其牧羊犬。它因主诉多尿和绞尿而被送往小动物医院。经检查，诊断为急性肝炎。医生对它进行了治疗（乌索福克、奶蓟草、泼尼松龙和 N 乙酰半胱氨酸）。治疗三天后，该病例失去了视力。眼科检查包括裂隙灯、希尔默泪液测试、眼压测量、荧光素染色和超声波检查。眼镜和眼底镜检查显示，视网膜锥体部位出现高反光和颜色变化，视网膜血管明显衰减，非锥体部位色素沉着，视盘萎缩且边缘呈扇形。眼底镜检查显示双眼视网膜变性。本病例报告旨在诊断土耳其牧羊犬的 PRA，强调其重要性，并与临床医生分享结果。
{"title":"Progressive retinal atrophy in Turkish Shepherd Dog; A case report","authors":"Mustafa Arican, E. Uzunlu, Yasemin Tanirli, Halil Caltiner, İremsu Satici","doi":"10.15312/eurasianjvetsci.2023.418","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.418","url":null,"abstract":"Progressive retinal atrophy (PRA) is described as an inherited degenerative disease of the retina that is common in dogs. The case was a 9-year-old female Turkish shepherd dog. It was presented to the Small Animal Hospital with complaints of polyuria and stranguria. After examination, the diagnosis was acute hepatitis. Medical treatment (ursofalk, milk thistle, prednisolone and N acetyl cystein) was given. The case lost vision after three days of treatment. The ophthalmological examinations were performed as included slit lamp, Schirmer's tear test, applanation tonometry, fluorescein staining and ultrasound. Ophthalmoscopic and fundoscopic examinations revealed hyperreflectivity and colour change in the tapetal region, marked attenuation of retinal vessels, depigmentation in the non-tapetal region, and optic disc atrophy with scalloped borders. Fundoscopic examination revealed retinal degeneration in both eyes. The aim of this case report is to diagnose PRA in Turkish shepherd dogs, to highlight its significance and to share the results with clinicians.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"17 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139185177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-07DOI: 10.15312/eurasianjvetsci.2022.385
Ozgur Kanat, M. Ortatatli
Amaç: Bu çalışma, köpeklerin bağırsaklarında meydana gelen patolojik değişiklikleri araştırmak, yaygınlıklarını tespit etmek ve ölüm nedeni olarak rollerini belirlemek amacıyla yapılmıştır. Gereç ve Yöntem: 100 ölü sokak köpeğin bağırsakları makroskopik, histopatolojik, mikrobiyolojik ve parazitik olarak incelendi. Parvovirüs ve distemper hastalıkları için immünohistokimyasal boyamalar da yapıldı. Bulgular: Köpeklerin 61'inde kanama, 8'inde ülser, 36'sında parazit, 14'ünde invaginasyon ve 2'sinde yabancı cisim tespit edildi. Mikroskobik olarak epitellerde dejenerasyon, nekroz ve deskuamasyon, bakteri kolonileri, propriyada hiperemi, kanama, fibrozis ve yangısal hücre infiltrasyonları, kriptlerde dejenerasyon, dilatasyon, hiperplazi, lenfoid dokuda boşalma gibi histopatolojik bulgular gözlendi ve her birinin şiddeti skorlandı. 100 köpekten 90'ında köpek parvovirüs pozitif IHC reaksiyonu ve 27 köpekte de distemper virüsü pozitif reaksiyonu bulundu. Mikrobiyolojik olarak 58 köpekte Campylobacter spp., E. coli, Enterococcus spp., Streptococcus spp. belirlendi. Histopatolojik olarak; sırasıyla 29 akut, 51 kronik, 15 lenfositik ve plazmasitik enteritis; kalın bağırsaklarda 5 akut, 10 kronik, 6 diffuz ve 20 idiopatik mukozal kolitis tanımlandı. Öneri: Köpeklerde, başta Parvoviral enteritis olmak üzere sindirim kanalı hastalıkları ve lezyonlarının birinci derecede ölüm sebebi olduğu ortaya konuldu. Genelde miks enfeksiyonların görüldüğü, sekonder etkenlerin enfeksiyona katılma dönemlerine göre lezyonların arttığı veya primer lezyonların maskelenmiş olduğu dikkati çekti. Anahtar kelimeler: Hastalıklar, immunohistokimya, ince ve kalın bağırsak, köpek, patoloji
{"title":"Detection of multiple etiologies and comparison and investigation of pathological changes in small and large intestine lesions of dogs","authors":"Ozgur Kanat, M. Ortatatli","doi":"10.15312/eurasianjvetsci.2022.385","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2022.385","url":null,"abstract":"Amaç: Bu çalışma, köpeklerin bağırsaklarında meydana gelen patolojik değişiklikleri araştırmak, yaygınlıklarını tespit etmek ve ölüm nedeni olarak rollerini belirlemek amacıyla yapılmıştır. Gereç ve Yöntem: 100 ölü sokak köpeğin bağırsakları makroskopik, histopatolojik, mikrobiyolojik ve parazitik olarak incelendi. Parvovirüs ve distemper hastalıkları için immünohistokimyasal boyamalar da yapıldı. Bulgular: Köpeklerin 61'inde kanama, 8'inde ülser, 36'sında parazit, 14'ünde invaginasyon ve 2'sinde yabancı cisim tespit edildi. Mikroskobik olarak epitellerde dejenerasyon, nekroz ve deskuamasyon, bakteri kolonileri, propriyada hiperemi, kanama, fibrozis ve yangısal hücre infiltrasyonları, kriptlerde dejenerasyon, dilatasyon, hiperplazi, lenfoid dokuda boşalma gibi histopatolojik bulgular gözlendi ve her birinin şiddeti skorlandı. 100 köpekten 90'ında köpek parvovirüs pozitif IHC reaksiyonu ve 27 köpekte de distemper virüsü pozitif reaksiyonu bulundu. Mikrobiyolojik olarak 58 köpekte Campylobacter spp., E. coli, Enterococcus spp., Streptococcus spp. belirlendi. Histopatolojik olarak; sırasıyla 29 akut, 51 kronik, 15 lenfositik ve plazmasitik enteritis; kalın bağırsaklarda 5 akut, 10 kronik, 6 diffuz ve 20 idiopatik mukozal kolitis tanımlandı. Öneri: Köpeklerde, başta Parvoviral enteritis olmak üzere sindirim kanalı hastalıkları ve lezyonlarının birinci derecede ölüm sebebi olduğu ortaya konuldu. Genelde miks enfeksiyonların görüldüğü, sekonder etkenlerin enfeksiyona katılma dönemlerine göre lezyonların arttığı veya primer lezyonların maskelenmiş olduğu dikkati çekti. Anahtar kelimeler: Hastalıklar, immunohistokimya, ince ve kalın bağırsak, köpek, patoloji","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88382247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: The aim of this study was to reveal the dimorphic structures of the foramen magnum and condyle occipitalis through an interspecies shape analysis and to determine the variability between sheep and goats. Materials and Methods: The study includes data from 81 skulls (46 sheep and 35 goat) for this aim. The foramen magnum frame shape and the condyle occipitalis variation were determined using type I (anatomical) and type III (semilandmarks). Results: Accordingly, was determined 30.76, 14.94 and 14.07 of the total shape variation of PC1, PC2 and PC3, respectively. It was determined that condylus occipitalis was primarily responsible for the shape variation according to PC1, and foramen magnum was responsible for the secondarly. It was found to explain the shape variation of the right condyle occipitalis to a great extent compared with PC2, while in PC3, it caused the entire right condyle occipitalis to explain the shape variation of the extreme lateral corner of the left condyle occipitalis and the left edge of the foramen magnum. The discriminant function analysis determined the Procrustes and Mahalanobis distances to be 0.12293879 (p < 0.0001) and 67.7482 (p < 0.0044), respectively. Conclusion: As a result, the geometric morphometry method is regarded to be a useful tool for detecting changes in skull shape between species and can thus be used successfully for taxonomic, archaeological, and forensic research.
{"title":"Geometric Morphometric Analysis of the Condylus Occipitalis and Foramen Magnum in Sheep and Goat","authors":"İsmail Demircioglu, Yasin Demiraslan, Funda Aksunger Karaavci, Iftar Gurbuz, Ozcan Ozgel","doi":"10.15312/eurasianjvetsci.2023.408","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.408","url":null,"abstract":"Aim: The aim of this study was to reveal the dimorphic structures of the foramen magnum and condyle occipitalis through an interspecies shape analysis and to determine the variability between sheep and goats. Materials and Methods: The study includes data from 81 skulls (46 sheep and 35 goat) for this aim. The foramen magnum frame shape and the condyle occipitalis variation were determined using type I (anatomical) and type III (semilandmarks). Results: Accordingly, was determined 30.76, 14.94 and 14.07 of the total shape variation of PC1, PC2 and PC3, respectively. It was determined that condylus occipitalis was primarily responsible for the shape variation according to PC1, and foramen magnum was responsible for the secondarly. It was found to explain the shape variation of the right condyle occipitalis to a great extent compared with PC2, while in PC3, it caused the entire right condyle occipitalis to explain the shape variation of the extreme lateral corner of the left condyle occipitalis and the left edge of the foramen magnum. The discriminant function analysis determined the Procrustes and Mahalanobis distances to be 0.12293879 (p < 0.0001) and 67.7482 (p < 0.0044), respectively. Conclusion: As a result, the geometric morphometry method is regarded to be a useful tool for detecting changes in skull shape between species and can thus be used successfully for taxonomic, archaeological, and forensic research.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.15312/eurasianjvetsci.2023.410
Ercan Erot, Mustafa Kose
Aim: This study was carried out to determine the prevalence of lungworm infections in small ruminants in the Uşak province. Materials and Methods: The study was carried out by testing the stool samples of 250 sheep and 250 goats collected from 5 different localities in the Uşak province using the Baermann-Wetzel method. Results: While the prevalence of lungworm infections was 9.6% in sheep, it was 34.4% in goats. The prevalence of infection was found to be 22% in all sheep and goats. While the prevalence of lungworm infections in sheep was determined to be 9.8% in females, no infection was found in males. The prevalence of infection in goats was determined as 33.3% in females and 53.8% in males. The prevalence of Muellerius capillaris was 5.6%, Dictyocaulus filaria 2%, Protostrongylus sp. 1.2% and Cystocaulus ocreatus 0.8% in sheep. In goats, the prevalence of M. capillaris was 34.4% and C. ocreatus was 0.4%. Mix infections caused by two lungworm species were detected in three sheep. However, mix infection was detected in only one goat. When the prevalence of lungworm infection in sheep was compared according to breeds, infection was detected in two of the five sheep breeds. The prevalence of lungworm infection was 14.1% in Eşme breed sheep and 9.1% in Kıvırcık breed sheep. Conclusion: The prevalence and risk status of lungworm infections in small ruminant breeding have been demonstrated. In addition, it has been determined that raising sheep and goats together and grazing animals from different age groups together may pose a risk in terms of lungworm infections.
{"title":"Lungworm infections in small ruminants in Uşak province","authors":"Ercan Erot, Mustafa Kose","doi":"10.15312/eurasianjvetsci.2023.410","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.410","url":null,"abstract":"Aim: This study was carried out to determine the prevalence of lungworm infections in small ruminants in the Uşak province. Materials and Methods: The study was carried out by testing the stool samples of 250 sheep and 250 goats collected from 5 different localities in the Uşak province using the Baermann-Wetzel method. Results: While the prevalence of lungworm infections was 9.6% in sheep, it was 34.4% in goats. The prevalence of infection was found to be 22% in all sheep and goats. While the prevalence of lungworm infections in sheep was determined to be 9.8% in females, no infection was found in males. The prevalence of infection in goats was determined as 33.3% in females and 53.8% in males. The prevalence of Muellerius capillaris was 5.6%, Dictyocaulus filaria 2%, Protostrongylus sp. 1.2% and Cystocaulus ocreatus 0.8% in sheep. In goats, the prevalence of M. capillaris was 34.4% and C. ocreatus was 0.4%. Mix infections caused by two lungworm species were detected in three sheep. However, mix infection was detected in only one goat. When the prevalence of lungworm infection in sheep was compared according to breeds, infection was detected in two of the five sheep breeds. The prevalence of lungworm infection was 14.1% in Eşme breed sheep and 9.1% in Kıvırcık breed sheep. Conclusion: The prevalence and risk status of lungworm infections in small ruminant breeding have been demonstrated. In addition, it has been determined that raising sheep and goats together and grazing animals from different age groups together may pose a risk in terms of lungworm infections.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.15312/eurasianjvetsci.2023.407
Mustafa Emin Oz, Oguzhan Avci, Muge Dogan
Aim: This study aimed to determine the deformed wing virus (DWV) master variants in managed honey bee hives in Central Anatolia and the Mediterranean Regions of Türkiye. Also, the relationship of DWV genotypes circulating in the apiaries with clinical signs observed in honey bee hives was investigated. Materials and Methods: For this study, adult honey bees were collected from the same 25 hives in the spring-summer and autumn seasons of 2019 from the provinces of Aksaray, Isparta, Karaman, Konya and Nigde. DWV-specific nucleic acid and DWV genotypes were detected by DWV real-time RT-PCR assay and ABC assay, respectively. Results: Deformed wing virus infection was detected in each sampling season. While many colonies were without any clinical signs, in some of the apiaries where samples were collected, wing deformity, trembling, paralysis, swelling in the abdomen, loss of productivity, and dead bees were observed. The prevalences of DWV-A, DWV-B, and DWV-C in adult honey bees were 62%, 82%, and 24%, respectively. The dominant genotype detected in bee hives was the DWV-B master variant (98%). Also, the virus load of the DWV-A master variant was high in all of the honey bee hives with wintering losses. Conclusion: In this present study, data on the current status of DWV master variants circulating in Turkey and their impacts on honey bee colonies are reported for the first time. Thus, it is thought that DWV, which causes yield losses at varying rates in every season of the year in Turkish bee hives, should be carefully monitored.
{"title":"Impact of deformed wing virus master variants (DWV-A, DWV-B, and DWV-C) in managed honey bee colonies of Türkiye","authors":"Mustafa Emin Oz, Oguzhan Avci, Muge Dogan","doi":"10.15312/eurasianjvetsci.2023.407","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.407","url":null,"abstract":"Aim: This study aimed to determine the deformed wing virus (DWV) master variants in managed honey bee hives in Central Anatolia and the Mediterranean Regions of Türkiye. Also, the relationship of DWV genotypes circulating in the apiaries with clinical signs observed in honey bee hives was investigated. Materials and Methods: For this study, adult honey bees were collected from the same 25 hives in the spring-summer and autumn seasons of 2019 from the provinces of Aksaray, Isparta, Karaman, Konya and Nigde. DWV-specific nucleic acid and DWV genotypes were detected by DWV real-time RT-PCR assay and ABC assay, respectively. Results: Deformed wing virus infection was detected in each sampling season. While many colonies were without any clinical signs, in some of the apiaries where samples were collected, wing deformity, trembling, paralysis, swelling in the abdomen, loss of productivity, and dead bees were observed. The prevalences of DWV-A, DWV-B, and DWV-C in adult honey bees were 62%, 82%, and 24%, respectively. The dominant genotype detected in bee hives was the DWV-B master variant (98%). Also, the virus load of the DWV-A master variant was high in all of the honey bee hives with wintering losses. Conclusion: In this present study, data on the current status of DWV master variants circulating in Turkey and their impacts on honey bee colonies are reported for the first time. Thus, it is thought that DWV, which causes yield losses at varying rates in every season of the year in Turkish bee hives, should be carefully monitored.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.15312/eurasianjvetsci.2023.405
Tahir Yilmaz, Egemen Gurdemir, Ayse Nizamlioglu, Yasin Akkemik, Ahmet Guner
Aim: The research was carried out to draw attention to online fresh meat sales and the precautions to be taken in this area by determining some quality characteristics of fresh meat sold online. Materials and Methods: The pH values of the samples were determined using a digital pH meter and the temperature values were determined using an infrared sensor-operated immersion thermometer. The color values of the samples were determined by measuring the L*, a*, and b* color values with a Chromameter color measuring device. The total viable counts was determined in Plate Count Agar medium, coliform bacteria number in Violet Red Bile Agar medium, Staphylococcus spp. number in Baird Parker Agar medium supplemented with Egg Yolk Tellurite Emulsion using classical culture techniques. Results: The mean pH values of pieced, cubed and minced meat purchased online and customers were respectively 5.62/5.62, 5.64/5.70, and 5.81/5.84. The average temperature values of pieced, cubed, and minced meat purchased online and customers were respectively 11.35/11.1°C, 11.26/11.7°C, and 12.07/12.7°C. Total viable counts of pieced, cubed, and minced meat purchased online and customers were respectively determined as 5.69/5.09, 6.34/5.68, and 7.01/6.36 log10 cfu/g. Temperature values and microbiological results determined in both meat samples purchased online with cold chain and customers without cold chain were similar. Conclusion: In light of the results, it is thought that the temperature values, packaging forms, and shipping conditions of online meat should be more prominent in the reorganization of legislation.
{"title":"Investigation of Some Physio-Chemical and Microbiological Quality of Fresh Meat Sold Online","authors":"Tahir Yilmaz, Egemen Gurdemir, Ayse Nizamlioglu, Yasin Akkemik, Ahmet Guner","doi":"10.15312/eurasianjvetsci.2023.405","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.405","url":null,"abstract":"Aim: The research was carried out to draw attention to online fresh meat sales and the precautions to be taken in this area by determining some quality characteristics of fresh meat sold online. Materials and Methods: The pH values of the samples were determined using a digital pH meter and the temperature values were determined using an infrared sensor-operated immersion thermometer. The color values of the samples were determined by measuring the L*, a*, and b* color values with a Chromameter color measuring device. The total viable counts was determined in Plate Count Agar medium, coliform bacteria number in Violet Red Bile Agar medium, Staphylococcus spp. number in Baird Parker Agar medium supplemented with Egg Yolk Tellurite Emulsion using classical culture techniques. Results: The mean pH values of pieced, cubed and minced meat purchased online and customers were respectively 5.62/5.62, 5.64/5.70, and 5.81/5.84. The average temperature values of pieced, cubed, and minced meat purchased online and customers were respectively 11.35/11.1°C, 11.26/11.7°C, and 12.07/12.7°C. Total viable counts of pieced, cubed, and minced meat purchased online and customers were respectively determined as 5.69/5.09, 6.34/5.68, and 7.01/6.36 log10 cfu/g. Temperature values and microbiological results determined in both meat samples purchased online with cold chain and customers without cold chain were similar. Conclusion: In light of the results, it is thought that the temperature values, packaging forms, and shipping conditions of online meat should be more prominent in the reorganization of legislation.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.15312/eurasianjvetsci.2023.409
Asli Balevi, Aysegul Ilban, Ali Uslu, Zafer Sayin, Ayten Gok, Beatriz Padron, Eda Toslak, Osman Erganis
Aim: Spontaneous capsular loss or capsular changes in Pasteurella multocida can result from repeated laboratory passages, positive or negative regulatory genes, or an unknown gene. This study, it was aimed to compare the properties of phenotypic, genotypic, and biotypic of each non-typical, and typical Pasteu-rella multocida strain, to determine the dominant genes on capsule synthesis. Materials and Methods: Fifty-six strains, which capsular type was determined, and thirty -six, which capsular type was not determined, were used in this study. Multiple linear regression analysis was used based on the presence of dominant genes (serogroup, serotype, toxin, adhesin, iron acquisition, and protectin) in the isolates. Results: When colony morphologies of strains were evaluated of these strains by culture methods, mucoid colony formation was commonly detected in typical strains (87.5%), in contrast to non-typical strains (27.7%). In typical strains, the highest percentages of ptfA, ompA, and tadD genes were 78.57%, 75%, and 69.64%, respectively. In non-typical strains, the highest rates of ompA, ptfA, and tadD genes were 61.1%, 52.78%, and 52.78%, respectively. According to multiple linear regression analysis results, the together hgbA with hgbB genes caused the increase of capsule synthesis in these strains. The presence of the ompA gene in these strains was secondly a induction on these strains. Other genes were not effective in capsule synthesis in these strains. Conclusion: It was determined that the most significant effect in the forming of non-typical P. multocida strains was related to not enough HgbA and HgbB genes. It was supposed that P. multocida may not be heavily encapsulated under iron-restricted conditions. Consequently, P. multocida may change its capsule or lose its capsule related to iron acquisition proteins.
{"title":"The enigmatical manipulators in the capsule synthesis of Pasteurella multocida: Iron acquisition proteins","authors":"Asli Balevi, Aysegul Ilban, Ali Uslu, Zafer Sayin, Ayten Gok, Beatriz Padron, Eda Toslak, Osman Erganis","doi":"10.15312/eurasianjvetsci.2023.409","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.409","url":null,"abstract":"Aim: Spontaneous capsular loss or capsular changes in Pasteurella multocida can result from repeated laboratory passages, positive or negative regulatory genes, or an unknown gene. This study, it was aimed to compare the properties of phenotypic, genotypic, and biotypic of each non-typical, and typical Pasteu-rella multocida strain, to determine the dominant genes on capsule synthesis. Materials and Methods: Fifty-six strains, which capsular type was determined, and thirty -six, which capsular type was not determined, were used in this study. Multiple linear regression analysis was used based on the presence of dominant genes (serogroup, serotype, toxin, adhesin, iron acquisition, and protectin) in the isolates. Results: When colony morphologies of strains were evaluated of these strains by culture methods, mucoid colony formation was commonly detected in typical strains (87.5%), in contrast to non-typical strains (27.7%). In typical strains, the highest percentages of ptfA, ompA, and tadD genes were 78.57%, 75%, and 69.64%, respectively. In non-typical strains, the highest rates of ompA, ptfA, and tadD genes were 61.1%, 52.78%, and 52.78%, respectively. According to multiple linear regression analysis results, the together hgbA with hgbB genes caused the increase of capsule synthesis in these strains. The presence of the ompA gene in these strains was secondly a induction on these strains. Other genes were not effective in capsule synthesis in these strains. Conclusion: It was determined that the most significant effect in the forming of non-typical P. multocida strains was related to not enough HgbA and HgbB genes. It was supposed that P. multocida may not be heavily encapsulated under iron-restricted conditions. Consequently, P. multocida may change its capsule or lose its capsule related to iron acquisition proteins.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.15312/eurasianjvetsci.2023.406
Emre Arslan, Orhan Cetin
Aim: This study was carried out to investigate the effect of different rearing conditions on the growth and fattening performance of American bronze turkeys Materials and Methods: In the study, data from a total of 123 American bronze turkeys were analyzed. The hatchlings, whose hatching weight was measured, were reared together for the first 10 weeks, and then divided into the experimental groups. The intensive rearing system group (control group) was fed only with concentrated ad libitum. Pasture groups are semi-intensive and extensive rearing systems. The semi-intensive group was subjected to 75% restricted feeding of the concentrate that the intensive group should consume. The turkey flock in the extensive rearing system was fed 50% of the feed that the turkeys in the intensive rearing system group should consume. Pasture groups benefited from the pasture between 09:00-17:00. The growth and fattening performances of the turkeys were examined by regular weighing and measurements every two weeks as they were being reared in these growing conditions between the ages of 10-32 weeks. Results: The 32-week live weights of turkeys were found to be 4.60-4.70 and 4.52 kg in females and 7.58-7.79 and 6.71 kg in males, in intensive, semi-intensive, and extensive systems, respectively. The effect of the rearing system was significant in males at 12-32 weeks, moreover, a difference was determined among groups in females at 14-28 weeks. Conclusion: As a result of, American bronze turkeys can be suitable breed for pasture conditions. Based on the live weight gain and feed efficiency values of the study groups, this breed can continue to grow for longer than 32 weeks when quality pastures are provided.
{"title":"Effects of Different Rearing Systems on Growth and Fattening Performance of American Bronze Turkeys","authors":"Emre Arslan, Orhan Cetin","doi":"10.15312/eurasianjvetsci.2023.406","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.406","url":null,"abstract":"Aim: This study was carried out to investigate the effect of different rearing conditions on the growth and fattening performance of American bronze turkeys Materials and Methods: In the study, data from a total of 123 American bronze turkeys were analyzed. The hatchlings, whose hatching weight was measured, were reared together for the first 10 weeks, and then divided into the experimental groups. The intensive rearing system group (control group) was fed only with concentrated ad libitum. Pasture groups are semi-intensive and extensive rearing systems. The semi-intensive group was subjected to 75% restricted feeding of the concentrate that the intensive group should consume. The turkey flock in the extensive rearing system was fed 50% of the feed that the turkeys in the intensive rearing system group should consume. Pasture groups benefited from the pasture between 09:00-17:00. The growth and fattening performances of the turkeys were examined by regular weighing and measurements every two weeks as they were being reared in these growing conditions between the ages of 10-32 weeks. Results: The 32-week live weights of turkeys were found to be 4.60-4.70 and 4.52 kg in females and 7.58-7.79 and 6.71 kg in males, in intensive, semi-intensive, and extensive systems, respectively. The effect of the rearing system was significant in males at 12-32 weeks, moreover, a difference was determined among groups in females at 14-28 weeks. Conclusion: As a result of, American bronze turkeys can be suitable breed for pasture conditions. Based on the live weight gain and feed efficiency values of the study groups, this breed can continue to grow for longer than 32 weeks when quality pastures are provided.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135363463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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