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Potential therapeutic effect of Thymoquinone on Cisplatin-induced hepatotoxicity in rats 胸腺醌对顺铂诱导的大鼠肝毒性的潜在治疗作用
Pub Date : 2024-03-08 DOI: 10.15312/eurasianjvetsci.2024.419
Sercan Kaya, T. Yalçın
Aim: Cisplatin (CIS), an antineoplastic agent, shows serious side effects such as hepatotoxicity. Thymoquinone (ThQ) is a powerful anti-oxidant with many beneficial effects such as anti-inflammatory, anti-tumoral. The aim of this study was to investigate whether ThQ treatment has hepatoprotective effect in CIS-induced hepatotoxicity model in rats and also to determine how CIS and/or ThQ treatments affect OTULIN levels, one of the deubiquitinases, in liver tissue.Materials and Methods: The 28 rats used in the study control (application not done), CIS (7 mg/kg CIS, first day of experiment, intraperitoneal), CIS+ThQ (7 mg/kg CIS, first day of experiment, intraperitoneal + 10 mg/kg/day ThQ, oral gavage) and ThQ (10 mg/kg/day ThQ, oral gavage) were divided into four equal groups (n=7). The experiment was terminated upon completion of all applications (day 15). The effects of CIS andor ThQ apps on liver tissues were examined biochemically, histopathologically and immunohistochemically. Results: CIS application increased liver enzyme levels, histopathological changes, inflammation, oxidative stress and apoptosis in liver tissue and caused a decrease in OTULIN level. However, ThQ administration showed a positive regulatory effect on CIS-induced changes in liver tissue.Conclusion: ThQ supplementation has a hepatoprotective effect against CIS-induced liver tissue damage.
目的:顺铂(CIS)是一种抗肿瘤药物,具有严重的副作用,如肝脏毒性。胸腺醌(ThQ)是一种强效抗氧化剂,具有抗炎、抗肿瘤等多种有益作用。本研究旨在探讨 ThQ 治疗在 CIS 诱导的大鼠肝毒性模型中是否具有保肝作用,并确定 CIS 和/或 ThQ 治疗如何影响肝组织中去泛素酶之一 OTULIN 的水平:研究中使用的 28 只大鼠分为 4 组(n=7),分别为对照组(未应用)、CIS 组(7 毫克/千克 CIS,实验第一天,腹腔注射)、CIS+ThQ 组(7 毫克/千克 CIS,实验第一天,腹腔注射 + 10 毫克/千克/天 ThQ,口服)和 ThQ 组(10 毫克/千克/天 ThQ,口服)。实验在所有应用结束后(第15天)终止。对CIS和ThQ应用对肝组织的影响进行生化、组织病理学和免疫组织化学检测。结果显示应用 CIS 会增加肝组织中的肝酶水平、组织病理学变化、炎症、氧化应激和细胞凋亡,并导致 OTULIN 水平下降。然而,服用 ThQ 对 CIS 引起的肝组织变化有积极的调节作用:结论:补充 ThQ 对 CIS 诱导的肝组织损伤具有保肝作用。
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引用次数: 0
Surgical treatment of Paraphimosis in dogs: 4 Cases 狗副阴茎畸形的手术治疗:4 例
Pub Date : 2024-03-08 DOI: 10.15312/eurasianjvetsci.2024.424
N. Zamirbekova, K. Parlak, Iremsu Satici, Hilmican Ergin, Sena Yazici, Ebrar Merve Eris, Melek Yildiz, Fahrettin Alkan
Aim: The aim of this study was to evaluate the use of surgical methods concerning the severity of persistent paraphimosis and describe the surgical techniques.Materials and Methods: The study material consisted of 4 dogs with paraphimosis. Based on anamnesis and clinical examination, 3 dogs were diagnosed with idiopathic permanent and one dog with traumatic paraphimosis. According to diagnoses and findings, patients underwent preputial advancement, partial penile amputation, phallopexy, and total penile amputation.Results: Recurrence of disease 1 week following surgery was recorded as postoperative complication in the first patient with preputial advancement. Intermittent bleeding in the surgical field in the second patient undergoing partial penile amputation continued for 3 days. No complications were observed as a result of the preputial advancement and phallopexy procedures performed for the treatment of the third case. The surgical wound healing time was recorded as one week in the second and third cases. In the fourth case, intermittent bleeding persisted for 12 days postoperatively and mild dermatitis was noted at the surgical site. According to the information received from the patient owners on the 30th day after the surgery, it was noted that there was no recurrence of the disease and no complications were encountered. Conclusion: According to the results of our study preputial advancement surgery technique alone was unsuccessful when the length of the prolapsed penis from the prepuce was 1,5 cm or more in patients with permanent paraphimosis. In addition, in cases where the disorder of the penile shaft and urethra allows, partial penile amputation should be preferred to total penile amputation.
目的:本研究旨在评估针对顽固性副阴茎炎严重程度的手术方法的使用情况,并描述手术技术:研究材料由 4 只患有副阴茎炎的狗组成。根据病史和临床检查,3 只犬被诊断为特发性永久性副阴茎畸形,1 只犬被诊断为外伤性副阴茎畸形。根据诊断和检查结果,患者接受了阴茎前端推进术、阴茎部分切除术、阴茎整形术和阴茎全切除术:第一例接受阴茎前庭推进术的患者术后并发症为术后一周疾病复发。第二位接受阴茎部分切除术的患者术野间歇性出血持续了 3 天。为治疗第三例患者而进行的阴茎前庭推进术和阴茎整形术均未发现并发症。第二例和第三例患者的手术伤口愈合时间均为一周。在第四个病例中,术后间歇性出血持续了 12 天,手术部位出现轻度皮炎。术后第 30 天,根据患者提供的信息,病情没有复发,也没有出现并发症。结论根据我们的研究结果,当永久性包皮过长患者的阴茎脱出包皮的长度达到或超过 1.5 厘米时,单纯的阴茎前突手术技术是不成功的。此外,在阴茎轴和尿道失调的情况下,阴茎部分截除术应优于阴茎全部截除术。
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引用次数: 0
Investigation of prevalence and risk factors of Parvovirus infection in dogs in Erzurum province, Turkey 土耳其埃尔祖鲁姆省犬只感染细小病毒的流行情况和风险因素调查
Pub Date : 2024-03-08 DOI: 10.15312/eurasianjvetsci.2024.421
Walied Fadulalseed Ahmed Ismail, B. Hanedan
Aim: This study aimed to investigate the prevalence and risk factors of canine parvovirus (CPV) infection in dogs that were presented to Animal Hospital of Atatürk University and housed in shelter. Materials and Methods: The samples were obtained from 83 dogs kept animal shelter and 17 dogs presented animal hospital showing clinical signs of CPV infection. The 40 stool samples of 100 dogs (40%) examined by the rapid test were positive for the presence of CPV, and the 60 stool samples (60%) were negative. Results: In this study, it was determined that there was an important association between the presence of CPV and vaccination status, housing place, cleanliness frequency of housing place, anthelmintic treatment as well as anorexia, vomiting, dehydration and abdominal pain findings. Conclusion: All the dogs in this study were young (1.5 to 7.5 months’ age range) and had contact with free-roaming stray dogs outside the house and garden. Thus, contact with stray dogs might play an important role in the increased prevalence of CPV. Also, the effective prevention practices should be implemented considering risk factors and common circulation of CPV infection in Erzurum province.
目的:本研究旨在调查阿塔图尔克大学动物医院收治的收容犬感染犬细小病毒 (CPV) 的流行率和风险因素。材料和方法:从动物收容所饲养的 83 只狗和动物医院就诊的 17 只狗身上采集样本,这些狗都有 CPV 感染的临床症状。用快速检测法检测了 100 只狗中的 40 份粪便样本(占 40%),结果显示 CPV 呈阳性,60 份粪便样本(占 60%)呈阴性。结果:在这项研究中,CPV 的存在与疫苗接种情况、饲养地点、饲养地点的清洁频率、驱虫治疗以及厌食、呕吐、脱水和腹痛结果之间存在重要关联。结论本研究中的所有犬只均为幼犬(1.5 至 7.5 月龄),曾在屋外和花园与自由活动的流浪狗接触。因此,与流浪狗的接触可能是 CPV 发病率上升的重要原因。此外,考虑到埃尔祖鲁姆省 CPV 感染的风险因素和常见传播途径,应采取有效的预防措施。
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引用次数: 0
Assessment of the protective effects of pomegranate peel extract and N-acetyl cysteine alone or in combination with Ornipural® against Cadmium-induced bone toxicity in rat 评估石榴皮提取物和 N-乙酰半胱氨酸单独或与 Ornipural® 联合使用对镉诱导的大鼠骨毒性的保护作用
Pub Date : 2024-03-08 DOI: 10.15312/eurasianjvetsci.2024.422
Durmus Hatipoglu, İsmail Demircioğlu, Kürşat Fi̇li̇kci̇, Yasemin Korkmaz, H. Gungor, Muhammed Demircioğlu, B. Dik
Aim: The present study was conducted to determine the effects of pomegranate peel extract (PPE) and N-acetyl Cysteine (NAC) alone or in their treatment combinations with commercial preparation Ornipural® (ORN) on bone metabolism against experimentally cadmium (Cd)-induced toxicity in rats. Materials and Methods: Totally 36 animals were used in the study including 6 Wistar Albino rats in each group. The animals were assigned to control, Cd, Cd+PPE, Cd+NAC, Cd+PPE+ORN and Cd+NAC+ORN groups. The animals in the groups were euthanized after their blood samples were taken at the end of the 8th week. The bones were subjected to maceration for morphometric and histopathological examinations after euthanasia.Results: The statistically significant differences were determined between the treatment groups and Cd group in terms of histopathological changes (osteoporotic alterations and changes in red bone marrow) and biomarkers (Ca, P and Mg) (P<0.05).Conclusion: Although PPE, NAC and treatment combinations with ORN applied against experimentally induced cadmium toxicity were determined to have positive effects on bone metabolism, it has been thought that carrying out trials by increasing treatment duration and dose would be beneficial to determine definite efficacy of the applied treatment protocols.
目的:本研究旨在确定石榴皮提取物(PPE)和 N-乙酰半胱氨酸(NAC)单独使用或与商业制剂 Ornipural® (ORN) 组合使用对大鼠骨代谢的影响,以防止镉(Cd)诱导的实验毒性。材料和方法:研究共使用了 36 只大鼠,每组 6 只 Wistar Albino 大鼠。动物被分为对照组、Cd 组、Cd+PPE 组、Cd+NAC 组、Cd+PPE+ORN 组和 Cd+NAC+ORN 组。各组动物在第 8 周结束时采集血样后安乐死。安乐死后对骨骼进行浸渍,以进行形态计量学和组织病理学检查:结果:治疗组与镉组在组织病理学变化(骨质疏松和红骨髓变化)和生物标志物(钙、磷和镁)方面差异有统计学意义(P<0.05):尽管针对实验诱导的镉毒性应用 PPE、NAC 和 ORN 治疗组合确定对骨代谢有积极影响,但认为通过延长治疗时间和增加剂量进行试验将有利于确定所应用治疗方案的确切疗效。
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引用次数: 0
Effect of hen age on egg quality, hatching performance and chick quality in Chukar Partridges (Alectoris chukar) 母鸡年龄对丘卡鹧鸪(Alectoris chukar)鸡蛋质量、孵化性能和雏鸟质量的影响
Pub Date : 2024-03-08 DOI: 10.15312/eurasianjvetsci.2024.423
Mustafa Çam, Mahmut Samil Samli, Z. K. Kaya, Halil Harman, Abdullah Bilir, K. Kırıkçı
Aim: This study was aimed to investigate the effect of different hen ages on egg quality characteristics, hatching performance and chick quality in chukar partridges.Materials and Methods: A total of 510 eggs obtained from chukar partridge flocks were used to determine the effects of different hen age on hatching performance, egg quality traits and chick quality. Daily collected 20 eggs from each age group (1, 2 and 3 years of age) were assessed for internal quality characteristics. A total of 150 eggs per each age group were set into incubator and hatcher set. After hatching, unhatched eggs were broken to determine hatching performance and embryonic mortality. Hatched day-old chicks were scored to determine chick quality characteristics.Results: Internal egg quality traits were not statistically affected by different breeding ages. Different breeding ages did not affect hatchability, early and middle embryonic mortality while late embryonic mortality and fertility of eggs were statistically affected. The evaluated chick quality traits were affected by the different hen age.Conclusion: Despite decreasing fertility rate with age, the results of this study suggest incubating eggs of chukar hens with 3 years age for optimum chick quality and being raised chukar hens up to 3 years of age.
目的:本研究旨在探讨不同鸡龄对鹧鸪蛋品质特征、孵化性能和雏鸟质量的影响:从鹧鸪群中共获得 510 枚蛋,用于测定不同鸡龄对孵化性能、蛋品质特征和雏鸟质量的影响。每天从每个龄组(1、2 和 3 龄)收集 20 枚鸡蛋,对其内部质量特征进行评估。每个日龄组共有 150 枚鸡蛋被放入孵化器中孵化。孵化后,对未孵化的蛋进行破壳,以确定孵化性能和胚胎死亡率。对孵出的日龄雏鸡进行评分,以确定雏鸡的质量特性:不同育种日龄对蛋的内部质量特征没有统计学影响。不同育种年龄对孵化率、早期和中期胚胎死亡率没有影响,而对晚期胚胎死亡率和鸡蛋的受精率有统计学影响。评估的雏鸡质量性状受不同鸡龄的影响:尽管受精率随鸡龄增长而下降,但本研究结果仍建议孵化 3 岁以上的蛋鸡,以获得最佳的雏鸡质量,并将蛋鸡饲养到 3 岁。
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引用次数: 0
Evaluation of antihyperlipidemic effect of rosemary oil in streptozotocin-induced diabetic rats 评估迷迭香油对链脲佐菌素诱导的糖尿病大鼠的降血脂作用
Pub Date : 2024-03-08 DOI: 10.15312/eurasianjvetsci.2024.420
D. Uluisik, E. Keskin
Aim: In this study, the potential effects of rosemary oil on the lipid profile in streptozotocin-induced diabetic rats were examined.Materials and Methods: In this study, thirty-two adult male Wistar Albino rats were used. Working groups were consisted as follows: control group (C, n=6), rosemary group (R, n=6), diabetes group (STZ, n=10) and diabetes+rosemary group (STZ+R, n=10). The rosemary oil was administered at oral dosage of 200 mg/kg daily for duration of three weeks in the groups receiving rosemary oil. Diabetes was performed by subcutaneous injections of streptozotocin at dosage of 40 mg/kg in 0.1 M citrate buffer (pH 4.5) for two consecutive days as a single daily dose. At the end of three weeks, blood samples collected from the all animals were assessed lipid parameters.Results: In this study, plasma levels of triglyceride, total cholesterol, and LDL-cholesterol significantly elevated, alongside plasma HDL-cholesterol levels in diabetes group significantly reduced compared to the control group. However, upon the application of rosemary oil to streptozotocin-induced diabetic rats, a substantial decrease in plasma triglyceride, total cholesterol, and LDL-cholesterol levels was observed, while plasma HDL-cholesterol notably increased.Conclusion: As a result of the current findings, it is concluded that rosemary oil may have antihiperlipidemic properties and could be used as a phytomedicine for the treatment of diabetes.
目的:本研究探讨了迷迭香油对链脲佐菌素诱导的糖尿病大鼠血脂谱的潜在影响:本研究使用 32 只成年雄性 Wistar Albino 大鼠。各组包括:对照组(C,n=6)、迷迭香组(R,n=6)、糖尿病组(STZ,n=10)和糖尿病+迷迭香组(STZ+R,n=10)。接受迷迭香油的组每天口服迷迭香油 200 毫克/千克,持续三周。糖尿病组则通过皮下注射链脲佐菌素,剂量为 40 毫克/公斤(0.1 M 柠檬酸盐缓冲液(pH 4.5)),连续两天,每天一次。三周后,对所有动物的血样进行血脂参数评估:结果:与对照组相比,糖尿病组的血浆甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平明显升高,血浆高密度脂蛋白胆固醇水平明显降低。然而,在链脲佐菌素诱导的糖尿病大鼠身上涂抹迷迭香油后,观察到血浆甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平大幅下降,而血浆高密度脂蛋白胆固醇则明显上升:结论:根据目前的研究结果,迷迭香油可能具有抗高血脂特性,可用作治疗糖尿病的植物药。
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引用次数: 0
Computed tomography reconstruction and morphometric analysis of the humerus and femur in New Zealand rabbits 新西兰兔肱骨和股骨的计算机断层扫描重建和形态计量分析
Pub Date : 2023-12-08 DOI: 10.15312/eurasianjvetsci.2023.413
M. L. Selcuk
Aim: This study was carried out to create three-dimensional (3D) models of the humerus and femur using computed tomography (CT) images of the New Zealand rabbit and to reveal whether there are differences between sexes and the right and left sides. Materials and Methods: The study was applied on 14 month old (10 male and 10 female) New Zealand rabbits. First, 0.5 mm thick images of the animals were taken by computed tomography (CT) and saved in DICOM format. Then, 3D models of the humerus and femur were obtained by reconstructing the images with the MIMICS 20.1 program. Morphometric data were obtained from the obtained 3D model. After this procedure, the rabbits were dissected and the weights of the humerus and femur were measured. Results: It was determined that the right humerus and femur volume and surface area in male rabbits were larger from the left side (p<0.05). There was no difference between the other morphometric values examined and rabbit bones did not show homotypic variation. In addition, it was thought that the humerus and femur bones could not be used to determine gender because the differences between the sexes were insignificant. Conclusion: It was thought that this study would provide the basis for studies and experimental models on rabbits by revealing the morphological features of the humerus and femur and would help the physician in the diagnosis of diseases by forming the basis of the data obtained with the imaging systems used in the clinical field.
目的:本研究旨在利用新西兰兔的计算机断层扫描(CT)图像创建肱骨和股骨的三维(3D)模型,并揭示性别和左右侧之间是否存在差异。材料和方法:研究对象为 14 个月大(10 雄 10 雌)的新西兰兔。首先,用计算机断层扫描(CT)拍摄动物 0.5 毫米厚的图像,并保存为 DICOM 格式。然后,用 MIMICS 20.1 程序重建图像,获得肱骨和股骨的三维模型。从获得的三维模型中获取形态测量数据。之后,解剖兔子并测量肱骨和股骨的重量。结果结果表明,雄兔右侧肱骨和股骨的体积和表面积均大于左侧(P<0.05)。其他形态测量值之间没有差异,兔子骨骼也没有同型变异。此外,由于肱骨和股骨的性别差异不明显,因此认为不能用肱骨和股骨来确定性别。结论这项研究通过揭示肱骨和股骨的形态特征,为兔子的研究和实验模型提供了依据,并通过形成临床领域使用的成像系统所获得的数据基础,帮助医生诊断疾病。
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引用次数: 0
A case of plasmacytoma with widespread metastasis in a dog 一例伴有广泛转移的狗浆细胞瘤
Pub Date : 2023-12-08 DOI: 10.15312/eurasianjvetsci.2023.417
Osman Dagar, Aysenur Tural, Zeynep Celik, Fatih Mehmet Ozturk, Mehmet Tuzcu, Erhan Gulcicek
In this study, a case of plasmacytoma with widespread metastasis in a nine-year-old male Rottweiler dog was described by histopathological and immunohistochemical methods. Macroscopic examination revealed multifocal white or pink-red colored masses ranging in size from 0,5 to 3 cm in the lung, liver, mediastinal and mesenterial lymph nodes, spleen, heart, pancreas, intestine and kidneys. In microscopic examination, tumor foci consisting mostly of atypical plasma cells were observed in the lung, liver, spleen, pancreas, mediastinal and mesenteric lymph nodes, intestine, and kidneys. Tumor-type giant cells were observed in tumor foci in the lung, mitotic figures in tumor foci in other organs, including the lung, and abundant tumor cells in capillary vessels. In Methyl Green Pyronin staining of lung, liver, spleen, pancreas, mediastinal and mesenterial lymph nodes, intestine and kidney sections, the cytoplasm of plasma cells was observed to be stained pink. Congo Red staining applied to liver and spleen sections showed amyloid deposits stained in brick red color. In addition, in the immunohistochemical staining of lung, liver, spleen, pancreas, mediastinal and mesenterial lymph nodes, intestine and kidney sections with Proliferating Cell Nuclear Antigen (PCNA) antibody, severe immunoreactivity was detected in tumor cells. As a result, due to the presence of rarely reported widespread metastases in the lung, liver, spleen, pancreas, mediastinal and mesenterial lymph nodes, intestine and kidneys of the diagnosed plasmacytoma case, it was deemed appropriate to publish the case in order to contribute to the literature by attracting the attention of veterinarians.
本研究采用组织病理学和免疫组化方法描述了一例9岁雄性罗威纳犬浆细胞瘤并广泛转移的病例。宏观检查发现,在肺、肝脏、纵隔和肠系膜淋巴结、脾脏、心脏、胰腺、肠道和肾脏中存在多灶性白色或粉红色肿块,大小从 0.5 厘米到 3 厘米不等。显微镜检查发现,肺、肝、脾、胰腺、纵隔和肠系膜淋巴结、肠道和肾脏中的肿瘤病灶主要由非典型浆细胞组成。在肺部的肿瘤灶中观察到肿瘤型巨细胞,在包括肺部在内的其他器官的肿瘤灶中观察到有丝分裂图形,在毛细血管中观察到大量肿瘤细胞。在肺部、肝脏、脾脏、胰腺、纵隔和肠系膜淋巴结、肠道和肾脏切片的甲基绿原酸染色中,浆细胞的细胞质被染成粉红色。肝脏和脾脏切片的刚果红染色显示淀粉样沉积物呈砖红色。此外,用增殖细胞核抗原(PCNA)抗体对肺、肝、脾、胰腺、纵隔和肠系膜淋巴结、肠和肾切片进行免疫组化染色时,在肿瘤细胞中发现了严重的免疫反应性。因此,鉴于该例浆细胞瘤在肺部、肝脏、脾脏、胰腺、纵隔和肠系膜淋巴结、肠道和肾脏出现广泛转移的罕见报道,我们认为应该公布该病例,以引起兽医的注意,为文献做出贡献。
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引用次数: 0
Investigation of Nosema apis and Nosema ceranae in bees by Multiplex PCR in Kırıkkale Region 利用多重 PCR 技术调查克勒克卡莱地区蜜蜂中的蜂鼻疽和陶瓷鼻疽病毒
Pub Date : 2023-12-08 DOI: 10.15312/eurasianjvetsci.2023.414
Meral Aydenizoz, Ali Can Yorulmaz
Aim: The aim of this study was conducted to determine the presence and prevalence of Nosemosis in honeybees in Kırıkkale region by Multiplex PCR. Materials and Methods: Samples were collected from 52 apiaries in Kırıkkale City Center, Bahşili, Balışeyh, Keskin, Sulakyurt and Yahşihan districts. The thorax and abdomen of ten bees collected from every 5 colonies were dissected and crushed with sterile physiological water in a porcelain mortar. The samples with Nosema spores were stained with Saffron and the multiplex PCR was applied to detect specimens to the same samples. Results: Nosema spp. spores were observed in 12 out of 52 apiaries (23.07%) microscopically. Among the positive samples, Balışeyh county was found with the highest rate of 57.14%. The township district is followed by Delice district with 33.33% and Kırıkkale /Center district with a ratio of 23.07%. Nosema spores were not found in samples taken from Bahşili, Keskin, Sulakyurt and Yahşihan districts. In general, the infection rates of the districts were determined as Central (5.76%), Balışeyh (7.69%), and Delice (9.61%). All the positive samples were detected as N. ceranae by multiplex PCR. Conclusion: This is the first study to determine the presence and prevalence of Nosemosis in honey bees in the Kırıkkale region and its etiological agent, N. ceranae. In line with the data obtained in this study, it was concluded that Nosemosis should be taken into consideration in bee losses in the region and beekeepers should be made aware of the issue.
目的:本研究旨在通过多重 PCR 检测 Kırıkkale 地区蜜蜂中诺塞病的存在和流行情况。材料和方法:从克勒克卡莱市中心、Bahşili、Balışeyh、Keskin、Sulakyurt 和 Yahşihan 地区的 52 个养蜂场采集样本。从每 5 个蜂群中采集 10 只蜜蜂的胸部和腹部进行解剖,并在瓷研钵中用无菌生理水进行粉碎。将带有诺斯玛孢子的样本用藏红花染色,并应用多重 PCR 检测同一样本的标本。结果在 52 个养蜂场中,有 12 个(23.07%)在显微镜下观察到了诺斯马属孢子。在阳性样本中,巴勒谢县的阳性率最高,为 57.14%。其次是德莱斯区(33.33%)和克勒克卡莱/中心区(23.07%)。在巴希利、凯斯金、苏拉尤尔特和亚希汉区采集的样本中未发现诺斯马孢子。总体而言,各地区的感染率分别为中部(5.76%)、巴勒谢(7.69%)和德莱斯(9.61%)。所有阳性样本均通过多重 PCR 检测出为 N. ceranae。结论这是首次研究确定 Kırıkkale 地区蜜蜂中诺塞姆病的存在和流行情况及其病原体 N. ceranae。根据这项研究获得的数据,得出的结论是,该地区的蜜蜂损失应考虑到诺塞米病,养蜂人也应意识到这一问题。
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引用次数: 0
Detection of bovine coronavirus (BCoV) infection in cattle with clinical respiratory signs by PCR and investigation of the serological status of these animals 通过 PCR 检测有临床呼吸道症状的牛是否感染了牛冠状病毒 (BCoV),并调查这些动物的血清学状况
Pub Date : 2023-12-08 DOI: 10.15312/eurasianjvetsci.2023.416
Meltem Menekse, Atilla Simsek
Aim: It was aimed to reveal the antigenic and serological prevalence of bovine coronavirus (BCoV) in cattle with clinically respiratory infection and to evaluate the relationship between virus shedding and serological status of animals in this study. Materials and Methods: In the study, nasal swab samples were collected from a total of 92 cattle of different gender between 0 and 2 years of age with clinical respiratory symptoms. These samples were subjected to RT-PCR using specific primer pairs. Furthermore, serum samples were also collected from the same animals and evaluated for BCoV-specific antibodies through an indirect ELISA method. Results: Ten (10.87%) swap samples were defined as positive for BCoV using RT-PCR. The seropositivity rate was 93.48% (86/92) in cattle by ELISA and 90% (9/10) in virus-shedding animals with nasal swab samples. Conclusion: The fact that 93.47% seropositivity was detected in unvaccinated animals against coronavirus in this study indicates that the animals were exposed to BCoV through colostrum or at a certain period of their lives. However the fact that 9 out of 10 animals with BCoV detected in swab samples also have BCoV-specific antibodies casts doubt on the efficacy of these antibodies in protecting against respiratory BCoV infections. We concluded that more detailed studies are needed to reveal the role of antibodies in respiratory coronavirus infections.
目的:本研究旨在揭示临床呼吸道感染牛的牛冠状病毒(BCoV)抗原和血清学流行情况,并评估病毒脱落与动物血清学状态之间的关系。材料和方法:本研究共采集了 92 头 0 至 2 岁有临床呼吸道症状的不同性别牛的鼻拭子样本。使用特定引物对这些样本进行 RT-PCR 检测。此外,还采集了相同动物的血清样本,并通过间接 ELISA 方法评估了 BCoV 特异性抗体。结果通过 RT-PCR 检测,10 份(10.87%)交换样本被确定为 BCoV 阳性。通过 ELISA 方法,牛的血清阳性率为 93.48%(86/92),通过鼻拭子样本,病毒感染动物的血清阳性率为 90%(9/10)。结论本研究在未接种冠状病毒疫苗的动物中检测到 93.47% 的血清阳性率,这表明这些动物通过初乳或在其生命的某个时期接触过 BCoV。然而,在拭子样本中检测到 BCoV 的 10 只动物中有 9 只也有 BCoV 特异性抗体,这让人怀疑这些抗体在预防呼吸道 BCoV 感染方面的功效。我们的结论是,需要进行更详细的研究,以揭示抗体在呼吸道冠状病毒感染中的作用。
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Eurasian Journal of Veterinary Sciences
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