Pub Date : 2024-03-08DOI: 10.15312/eurasianjvetsci.2024.419
Sercan Kaya, T. Yalçın
Aim: Cisplatin (CIS), an antineoplastic agent, shows serious side effects such as hepatotoxicity. Thymoquinone (ThQ) is a powerful anti-oxidant with many beneficial effects such as anti-inflammatory, anti-tumoral. The aim of this study was to investigate whether ThQ treatment has hepatoprotective effect in CIS-induced hepatotoxicity model in rats and also to determine how CIS and/or ThQ treatments affect OTULIN levels, one of the deubiquitinases, in liver tissue. Materials and Methods: The 28 rats used in the study control (application not done), CIS (7 mg/kg CIS, first day of experiment, intraperitoneal), CIS+ThQ (7 mg/kg CIS, first day of experiment, intraperitoneal + 10 mg/kg/day ThQ, oral gavage) and ThQ (10 mg/kg/day ThQ, oral gavage) were divided into four equal groups (n=7). The experiment was terminated upon completion of all applications (day 15). The effects of CIS andor ThQ apps on liver tissues were examined biochemically, histopathologically and immunohistochemically. Results: CIS application increased liver enzyme levels, histopathological changes, inflammation, oxidative stress and apoptosis in liver tissue and caused a decrease in OTULIN level. However, ThQ administration showed a positive regulatory effect on CIS-induced changes in liver tissue. Conclusion: ThQ supplementation has a hepatoprotective effect against CIS-induced liver tissue damage.
{"title":"Potential therapeutic effect of Thymoquinone on Cisplatin-induced hepatotoxicity in rats","authors":"Sercan Kaya, T. Yalçın","doi":"10.15312/eurasianjvetsci.2024.419","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2024.419","url":null,"abstract":"Aim: Cisplatin (CIS), an antineoplastic agent, shows serious side effects such as hepatotoxicity. Thymoquinone (ThQ) is a powerful anti-oxidant with many beneficial effects such as anti-inflammatory, anti-tumoral. The aim of this study was to investigate whether ThQ treatment has hepatoprotective effect in CIS-induced hepatotoxicity model in rats and also to determine how CIS and/or ThQ treatments affect OTULIN levels, one of the deubiquitinases, in liver tissue.\u0000\u0000Materials and Methods: The 28 rats used in the study control (application not done), CIS (7 mg/kg CIS, first day of experiment, intraperitoneal), CIS+ThQ (7 mg/kg CIS, first day of experiment, intraperitoneal + 10 mg/kg/day ThQ, oral gavage) and ThQ (10 mg/kg/day ThQ, oral gavage) were divided into four equal groups (n=7). The experiment was terminated upon completion of all applications (day 15). The effects of CIS andor ThQ apps on liver tissues were examined biochemically, histopathologically and immunohistochemically. \u0000\u0000Results: CIS application increased liver enzyme levels, histopathological changes, inflammation, oxidative stress and apoptosis in liver tissue and caused a decrease in OTULIN level. However, ThQ administration showed a positive regulatory effect on CIS-induced changes in liver tissue.\u0000\u0000Conclusion: ThQ supplementation has a hepatoprotective effect against CIS-induced liver tissue damage.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"89 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-08DOI: 10.15312/eurasianjvetsci.2024.424
N. Zamirbekova, K. Parlak, Iremsu Satici, Hilmican Ergin, Sena Yazici, Ebrar Merve Eris, Melek Yildiz, Fahrettin Alkan
Aim: The aim of this study was to evaluate the use of surgical methods concerning the severity of persistent paraphimosis and describe the surgical techniques. Materials and Methods: The study material consisted of 4 dogs with paraphimosis. Based on anamnesis and clinical examination, 3 dogs were diagnosed with idiopathic permanent and one dog with traumatic paraphimosis. According to diagnoses and findings, patients underwent preputial advancement, partial penile amputation, phallopexy, and total penile amputation. Results: Recurrence of disease 1 week following surgery was recorded as postoperative complication in the first patient with preputial advancement. Intermittent bleeding in the surgical field in the second patient undergoing partial penile amputation continued for 3 days. No complications were observed as a result of the preputial advancement and phallopexy procedures performed for the treatment of the third case. The surgical wound healing time was recorded as one week in the second and third cases. In the fourth case, intermittent bleeding persisted for 12 days postoperatively and mild dermatitis was noted at the surgical site. According to the information received from the patient owners on the 30th day after the surgery, it was noted that there was no recurrence of the disease and no complications were encountered. Conclusion: According to the results of our study preputial advancement surgery technique alone was unsuccessful when the length of the prolapsed penis from the prepuce was 1,5 cm or more in patients with permanent paraphimosis. In addition, in cases where the disorder of the penile shaft and urethra allows, partial penile amputation should be preferred to total penile amputation.
{"title":"Surgical treatment of Paraphimosis in dogs: 4 Cases","authors":"N. Zamirbekova, K. Parlak, Iremsu Satici, Hilmican Ergin, Sena Yazici, Ebrar Merve Eris, Melek Yildiz, Fahrettin Alkan","doi":"10.15312/eurasianjvetsci.2024.424","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2024.424","url":null,"abstract":"Aim: The aim of this study was to evaluate the use of surgical methods concerning the severity of persistent paraphimosis and describe the surgical techniques.\u0000\u0000Materials and Methods: The study material consisted of 4 dogs with paraphimosis. Based on anamnesis and clinical examination, 3 dogs were diagnosed with idiopathic permanent and one dog with traumatic paraphimosis. According to diagnoses and findings, patients underwent preputial advancement, partial penile amputation, phallopexy, and total penile amputation.\u0000\u0000Results: Recurrence of disease 1 week following surgery was recorded as postoperative complication in the first patient with preputial advancement. Intermittent bleeding in the surgical field in the second patient undergoing partial penile amputation continued for 3 days. No complications were observed as a result of the preputial advancement and phallopexy procedures performed for the treatment of the third case. The surgical wound healing time was recorded as one week in the second and third cases. In the fourth case, intermittent bleeding persisted for 12 days postoperatively and mild dermatitis was noted at the surgical site. According to the information received from the patient owners on the 30th day after the surgery, it was noted that there was no recurrence of the disease and no complications were encountered. \u0000\u0000Conclusion: According to the results of our study preputial advancement surgery technique alone was unsuccessful when the length of the prolapsed penis from the prepuce was 1,5 cm or more in patients with permanent paraphimosis. In addition, in cases where the disorder of the penile shaft and urethra allows, partial penile amputation should be preferred to total penile amputation.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"45 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-08DOI: 10.15312/eurasianjvetsci.2024.421
Walied Fadulalseed Ahmed Ismail, B. Hanedan
Aim: This study aimed to investigate the prevalence and risk factors of canine parvovirus (CPV) infection in dogs that were presented to Animal Hospital of Atatürk University and housed in shelter. Materials and Methods: The samples were obtained from 83 dogs kept animal shelter and 17 dogs presented animal hospital showing clinical signs of CPV infection. The 40 stool samples of 100 dogs (40%) examined by the rapid test were positive for the presence of CPV, and the 60 stool samples (60%) were negative. Results: In this study, it was determined that there was an important association between the presence of CPV and vaccination status, housing place, cleanliness frequency of housing place, anthelmintic treatment as well as anorexia, vomiting, dehydration and abdominal pain findings. Conclusion: All the dogs in this study were young (1.5 to 7.5 months’ age range) and had contact with free-roaming stray dogs outside the house and garden. Thus, contact with stray dogs might play an important role in the increased prevalence of CPV. Also, the effective prevention practices should be implemented considering risk factors and common circulation of CPV infection in Erzurum province.
{"title":"Investigation of prevalence and risk factors of Parvovirus infection in dogs in Erzurum province, Turkey","authors":"Walied Fadulalseed Ahmed Ismail, B. Hanedan","doi":"10.15312/eurasianjvetsci.2024.421","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2024.421","url":null,"abstract":"Aim: This study aimed to investigate the prevalence and risk factors of canine parvovirus (CPV) infection in dogs that were presented to Animal Hospital of Atatürk University and housed in shelter. \u0000\u0000Materials and Methods: The samples were obtained from 83 dogs kept animal shelter and 17 dogs presented animal hospital showing clinical signs of CPV infection. The 40 stool samples of 100 dogs (40%) examined by the rapid test were positive for the presence of CPV, and the 60 stool samples (60%) were negative. \u0000\u0000Results: In this study, it was determined that there was an important association between the presence of CPV and vaccination status, housing place, cleanliness frequency of housing place, anthelmintic treatment as well as anorexia, vomiting, dehydration and abdominal pain findings. \u0000\u0000Conclusion: All the dogs in this study were young (1.5 to 7.5 months’ age range) and had contact with free-roaming stray dogs outside the house and garden. Thus, contact with stray dogs might play an important role in the increased prevalence of CPV. Also, the effective prevention practices should be implemented considering risk factors and common circulation of CPV infection in Erzurum province.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"24 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140258105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-08DOI: 10.15312/eurasianjvetsci.2024.422
Durmus Hatipoglu, İsmail Demircioğlu, Kürşat Fi̇li̇kci̇, Yasemin Korkmaz, H. Gungor, Muhammed Demircioğlu, B. Dik
Aim: The present study was conducted to determine the effects of pomegranate peel extract (PPE) and N-acetyl Cysteine (NAC) alone or in their treatment combinations with commercial preparation Ornipural® (ORN) on bone metabolism against experimentally cadmium (Cd)-induced toxicity in rats. Materials and Methods: Totally 36 animals were used in the study including 6 Wistar Albino rats in each group. The animals were assigned to control, Cd, Cd+PPE, Cd+NAC, Cd+PPE+ORN and Cd+NAC+ORN groups. The animals in the groups were euthanized after their blood samples were taken at the end of the 8th week. The bones were subjected to maceration for morphometric and histopathological examinations after euthanasia. Results: The statistically significant differences were determined between the treatment groups and Cd group in terms of histopathological changes (osteoporotic alterations and changes in red bone marrow) and biomarkers (Ca, P and Mg) (P<0.05). Conclusion: Although PPE, NAC and treatment combinations with ORN applied against experimentally induced cadmium toxicity were determined to have positive effects on bone metabolism, it has been thought that carrying out trials by increasing treatment duration and dose would be beneficial to determine definite efficacy of the applied treatment protocols.
{"title":"Assessment of the protective effects of pomegranate peel extract and N-acetyl cysteine alone or in combination with Ornipural® against Cadmium-induced bone toxicity in rat","authors":"Durmus Hatipoglu, İsmail Demircioğlu, Kürşat Fi̇li̇kci̇, Yasemin Korkmaz, H. Gungor, Muhammed Demircioğlu, B. Dik","doi":"10.15312/eurasianjvetsci.2024.422","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2024.422","url":null,"abstract":"Aim: The present study was conducted to determine the effects of pomegranate peel extract (PPE) and N-acetyl Cysteine (NAC) alone or in their treatment combinations with commercial preparation Ornipural® (ORN) on bone metabolism against experimentally cadmium (Cd)-induced toxicity in rats. \u0000\u0000Materials and Methods: Totally 36 animals were used in the study including 6 Wistar Albino rats in each group. The animals were assigned to control, Cd, Cd+PPE, Cd+NAC, Cd+PPE+ORN and Cd+NAC+ORN groups. The animals in the groups were euthanized after their blood samples were taken at the end of the 8th week. The bones were subjected to maceration for morphometric and histopathological examinations after euthanasia.\u0000\u0000Results: The statistically significant differences were determined between the treatment groups and Cd group in terms of histopathological changes (osteoporotic alterations and changes in red bone marrow) and biomarkers (Ca, P and Mg) (P<0.05).\u0000\u0000Conclusion: Although PPE, NAC and treatment combinations with ORN applied against experimentally induced cadmium toxicity were determined to have positive effects on bone metabolism, it has been thought that carrying out trials by increasing treatment duration and dose would be beneficial to determine definite efficacy of the applied treatment protocols.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"27 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-08DOI: 10.15312/eurasianjvetsci.2024.423
Mustafa Çam, Mahmut Samil Samli, Z. K. Kaya, Halil Harman, Abdullah Bilir, K. Kırıkçı
Aim: This study was aimed to investigate the effect of different hen ages on egg quality characteristics, hatching performance and chick quality in chukar partridges. Materials and Methods: A total of 510 eggs obtained from chukar partridge flocks were used to determine the effects of different hen age on hatching performance, egg quality traits and chick quality. Daily collected 20 eggs from each age group (1, 2 and 3 years of age) were assessed for internal quality characteristics. A total of 150 eggs per each age group were set into incubator and hatcher set. After hatching, unhatched eggs were broken to determine hatching performance and embryonic mortality. Hatched day-old chicks were scored to determine chick quality characteristics. Results: Internal egg quality traits were not statistically affected by different breeding ages. Different breeding ages did not affect hatchability, early and middle embryonic mortality while late embryonic mortality and fertility of eggs were statistically affected. The evaluated chick quality traits were affected by the different hen age. Conclusion: Despite decreasing fertility rate with age, the results of this study suggest incubating eggs of chukar hens with 3 years age for optimum chick quality and being raised chukar hens up to 3 years of age.
{"title":"Effect of hen age on egg quality, hatching performance and chick quality in Chukar Partridges (Alectoris chukar)","authors":"Mustafa Çam, Mahmut Samil Samli, Z. K. Kaya, Halil Harman, Abdullah Bilir, K. Kırıkçı","doi":"10.15312/eurasianjvetsci.2024.423","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2024.423","url":null,"abstract":"Aim: This study was aimed to investigate the effect of different hen ages on egg quality characteristics, hatching performance and chick quality in chukar partridges.\u0000\u0000Materials and Methods: A total of 510 eggs obtained from chukar partridge flocks were used to determine the effects of different hen age on hatching performance, egg quality traits and chick quality. Daily collected 20 eggs from each age group (1, 2 and 3 years of age) were assessed for internal quality characteristics. A total of 150 eggs per each age group were set into incubator and hatcher set. After hatching, unhatched eggs were broken to determine hatching performance and embryonic mortality. Hatched day-old chicks were scored to determine chick quality characteristics.\u0000\u0000Results: Internal egg quality traits were not statistically affected by different breeding ages. Different breeding ages did not affect hatchability, early and middle embryonic mortality while late embryonic mortality and fertility of eggs were statistically affected. The evaluated chick quality traits were affected by the different hen age.\u0000\u0000Conclusion: Despite decreasing fertility rate with age, the results of this study suggest incubating eggs of chukar hens with 3 years age for optimum chick quality and being raised chukar hens up to 3 years of age.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"12 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-08DOI: 10.15312/eurasianjvetsci.2024.420
D. Uluisik, E. Keskin
Aim: In this study, the potential effects of rosemary oil on the lipid profile in streptozotocin-induced diabetic rats were examined. Materials and Methods: In this study, thirty-two adult male Wistar Albino rats were used. Working groups were consisted as follows: control group (C, n=6), rosemary group (R, n=6), diabetes group (STZ, n=10) and diabetes+rosemary group (STZ+R, n=10). The rosemary oil was administered at oral dosage of 200 mg/kg daily for duration of three weeks in the groups receiving rosemary oil. Diabetes was performed by subcutaneous injections of streptozotocin at dosage of 40 mg/kg in 0.1 M citrate buffer (pH 4.5) for two consecutive days as a single daily dose. At the end of three weeks, blood samples collected from the all animals were assessed lipid parameters. Results: In this study, plasma levels of triglyceride, total cholesterol, and LDL-cholesterol significantly elevated, alongside plasma HDL-cholesterol levels in diabetes group significantly reduced compared to the control group. However, upon the application of rosemary oil to streptozotocin-induced diabetic rats, a substantial decrease in plasma triglyceride, total cholesterol, and LDL-cholesterol levels was observed, while plasma HDL-cholesterol notably increased. Conclusion: As a result of the current findings, it is concluded that rosemary oil may have antihiperlipidemic properties and could be used as a phytomedicine for the treatment of diabetes.
{"title":"Evaluation of antihyperlipidemic effect of rosemary oil in streptozotocin-induced diabetic rats","authors":"D. Uluisik, E. Keskin","doi":"10.15312/eurasianjvetsci.2024.420","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2024.420","url":null,"abstract":"Aim: In this study, the potential effects of rosemary oil on the lipid profile in streptozotocin-induced diabetic rats were examined.\u0000\u0000Materials and Methods: In this study, thirty-two adult male Wistar Albino rats were used. Working groups were consisted as follows: control group (C, n=6), rosemary group (R, n=6), diabetes group (STZ, n=10) and diabetes+rosemary group (STZ+R, n=10). The rosemary oil was administered at oral dosage of 200 mg/kg daily for duration of three weeks in the groups receiving rosemary oil. Diabetes was performed by subcutaneous injections of streptozotocin at dosage of 40 mg/kg in 0.1 M citrate buffer (pH 4.5) for two consecutive days as a single daily dose. At the end of three weeks, blood samples collected from the all animals were assessed lipid parameters.\u0000\u0000Results: In this study, plasma levels of triglyceride, total cholesterol, and LDL-cholesterol significantly elevated, alongside plasma HDL-cholesterol levels in diabetes group significantly reduced compared to the control group. However, upon the application of rosemary oil to streptozotocin-induced diabetic rats, a substantial decrease in plasma triglyceride, total cholesterol, and LDL-cholesterol levels was observed, while plasma HDL-cholesterol notably increased.\u0000\u0000Conclusion: As a result of the current findings, it is concluded that rosemary oil may have antihiperlipidemic properties and could be used as a phytomedicine for the treatment of diabetes.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"112 S140","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.15312/eurasianjvetsci.2023.413
M. L. Selcuk
Aim: This study was carried out to create three-dimensional (3D) models of the humerus and femur using computed tomography (CT) images of the New Zealand rabbit and to reveal whether there are differences between sexes and the right and left sides. Materials and Methods: The study was applied on 14 month old (10 male and 10 female) New Zealand rabbits. First, 0.5 mm thick images of the animals were taken by computed tomography (CT) and saved in DICOM format. Then, 3D models of the humerus and femur were obtained by reconstructing the images with the MIMICS 20.1 program. Morphometric data were obtained from the obtained 3D model. After this procedure, the rabbits were dissected and the weights of the humerus and femur were measured. Results: It was determined that the right humerus and femur volume and surface area in male rabbits were larger from the left side (p<0.05). There was no difference between the other morphometric values examined and rabbit bones did not show homotypic variation. In addition, it was thought that the humerus and femur bones could not be used to determine gender because the differences between the sexes were insignificant. Conclusion: It was thought that this study would provide the basis for studies and experimental models on rabbits by revealing the morphological features of the humerus and femur and would help the physician in the diagnosis of diseases by forming the basis of the data obtained with the imaging systems used in the clinical field.
{"title":"Computed tomography reconstruction and morphometric analysis of the humerus and femur in New Zealand rabbits","authors":"M. L. Selcuk","doi":"10.15312/eurasianjvetsci.2023.413","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.413","url":null,"abstract":"Aim: This study was carried out to create three-dimensional (3D) models of the humerus and femur using computed tomography (CT) images of the New Zealand rabbit and to reveal whether there are differences between sexes and the right and left sides. Materials and Methods: The study was applied on 14 month old (10 male and 10 female) New Zealand rabbits. First, 0.5 mm thick images of the animals were taken by computed tomography (CT) and saved in DICOM format. Then, 3D models of the humerus and femur were obtained by reconstructing the images with the MIMICS 20.1 program. Morphometric data were obtained from the obtained 3D model. After this procedure, the rabbits were dissected and the weights of the humerus and femur were measured. Results: It was determined that the right humerus and femur volume and surface area in male rabbits were larger from the left side (p<0.05). There was no difference between the other morphometric values examined and rabbit bones did not show homotypic variation. In addition, it was thought that the humerus and femur bones could not be used to determine gender because the differences between the sexes were insignificant. Conclusion: It was thought that this study would provide the basis for studies and experimental models on rabbits by revealing the morphological features of the humerus and femur and would help the physician in the diagnosis of diseases by forming the basis of the data obtained with the imaging systems used in the clinical field.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139185166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.15312/eurasianjvetsci.2023.417
Osman Dagar, Aysenur Tural, Zeynep Celik, Fatih Mehmet Ozturk, Mehmet Tuzcu, Erhan Gulcicek
In this study, a case of plasmacytoma with widespread metastasis in a nine-year-old male Rottweiler dog was described by histopathological and immunohistochemical methods. Macroscopic examination revealed multifocal white or pink-red colored masses ranging in size from 0,5 to 3 cm in the lung, liver, mediastinal and mesenterial lymph nodes, spleen, heart, pancreas, intestine and kidneys. In microscopic examination, tumor foci consisting mostly of atypical plasma cells were observed in the lung, liver, spleen, pancreas, mediastinal and mesenteric lymph nodes, intestine, and kidneys. Tumor-type giant cells were observed in tumor foci in the lung, mitotic figures in tumor foci in other organs, including the lung, and abundant tumor cells in capillary vessels. In Methyl Green Pyronin staining of lung, liver, spleen, pancreas, mediastinal and mesenterial lymph nodes, intestine and kidney sections, the cytoplasm of plasma cells was observed to be stained pink. Congo Red staining applied to liver and spleen sections showed amyloid deposits stained in brick red color. In addition, in the immunohistochemical staining of lung, liver, spleen, pancreas, mediastinal and mesenterial lymph nodes, intestine and kidney sections with Proliferating Cell Nuclear Antigen (PCNA) antibody, severe immunoreactivity was detected in tumor cells. As a result, due to the presence of rarely reported widespread metastases in the lung, liver, spleen, pancreas, mediastinal and mesenterial lymph nodes, intestine and kidneys of the diagnosed plasmacytoma case, it was deemed appropriate to publish the case in order to contribute to the literature by attracting the attention of veterinarians.
{"title":"A case of plasmacytoma with widespread metastasis in a dog","authors":"Osman Dagar, Aysenur Tural, Zeynep Celik, Fatih Mehmet Ozturk, Mehmet Tuzcu, Erhan Gulcicek","doi":"10.15312/eurasianjvetsci.2023.417","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.417","url":null,"abstract":"In this study, a case of plasmacytoma with widespread metastasis in a nine-year-old male Rottweiler dog was described by histopathological and immunohistochemical methods. Macroscopic examination revealed multifocal white or pink-red colored masses ranging in size from 0,5 to 3 cm in the lung, liver, mediastinal and mesenterial lymph nodes, spleen, heart, pancreas, intestine and kidneys. In microscopic examination, tumor foci consisting mostly of atypical plasma cells were observed in the lung, liver, spleen, pancreas, mediastinal and mesenteric lymph nodes, intestine, and kidneys. Tumor-type giant cells were observed in tumor foci in the lung, mitotic figures in tumor foci in other organs, including the lung, and abundant tumor cells in capillary vessels. In Methyl Green Pyronin staining of lung, liver, spleen, pancreas, mediastinal and mesenterial lymph nodes, intestine and kidney sections, the cytoplasm of plasma cells was observed to be stained pink. Congo Red staining applied to liver and spleen sections showed amyloid deposits stained in brick red color. In addition, in the immunohistochemical staining of lung, liver, spleen, pancreas, mediastinal and mesenterial lymph nodes, intestine and kidney sections with Proliferating Cell Nuclear Antigen (PCNA) antibody, severe immunoreactivity was detected in tumor cells. As a result, due to the presence of rarely reported widespread metastases in the lung, liver, spleen, pancreas, mediastinal and mesenterial lymph nodes, intestine and kidneys of the diagnosed plasmacytoma case, it was deemed appropriate to publish the case in order to contribute to the literature by attracting the attention of veterinarians.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"6 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139185028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.15312/eurasianjvetsci.2023.414
Meral Aydenizoz, Ali Can Yorulmaz
Aim: The aim of this study was conducted to determine the presence and prevalence of Nosemosis in honeybees in Kırıkkale region by Multiplex PCR. Materials and Methods: Samples were collected from 52 apiaries in Kırıkkale City Center, Bahşili, Balışeyh, Keskin, Sulakyurt and Yahşihan districts. The thorax and abdomen of ten bees collected from every 5 colonies were dissected and crushed with sterile physiological water in a porcelain mortar. The samples with Nosema spores were stained with Saffron and the multiplex PCR was applied to detect specimens to the same samples. Results: Nosema spp. spores were observed in 12 out of 52 apiaries (23.07%) microscopically. Among the positive samples, Balışeyh county was found with the highest rate of 57.14%. The township district is followed by Delice district with 33.33% and Kırıkkale /Center district with a ratio of 23.07%. Nosema spores were not found in samples taken from Bahşili, Keskin, Sulakyurt and Yahşihan districts. In general, the infection rates of the districts were determined as Central (5.76%), Balışeyh (7.69%), and Delice (9.61%). All the positive samples were detected as N. ceranae by multiplex PCR. Conclusion: This is the first study to determine the presence and prevalence of Nosemosis in honey bees in the Kırıkkale region and its etiological agent, N. ceranae. In line with the data obtained in this study, it was concluded that Nosemosis should be taken into consideration in bee losses in the region and beekeepers should be made aware of the issue.
{"title":"Investigation of Nosema apis and Nosema ceranae in bees by Multiplex PCR in Kırıkkale Region","authors":"Meral Aydenizoz, Ali Can Yorulmaz","doi":"10.15312/eurasianjvetsci.2023.414","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.414","url":null,"abstract":"Aim: The aim of this study was conducted to determine the presence and prevalence of Nosemosis in honeybees in Kırıkkale region by Multiplex PCR. Materials and Methods: Samples were collected from 52 apiaries in Kırıkkale City Center, Bahşili, Balışeyh, Keskin, Sulakyurt and Yahşihan districts. The thorax and abdomen of ten bees collected from every 5 colonies were dissected and crushed with sterile physiological water in a porcelain mortar. The samples with Nosema spores were stained with Saffron and the multiplex PCR was applied to detect specimens to the same samples. Results: Nosema spp. spores were observed in 12 out of 52 apiaries (23.07%) microscopically. Among the positive samples, Balışeyh county was found with the highest rate of 57.14%. The township district is followed by Delice district with 33.33% and Kırıkkale /Center district with a ratio of 23.07%. Nosema spores were not found in samples taken from Bahşili, Keskin, Sulakyurt and Yahşihan districts. In general, the infection rates of the districts were determined as Central (5.76%), Balışeyh (7.69%), and Delice (9.61%). All the positive samples were detected as N. ceranae by multiplex PCR. Conclusion: This is the first study to determine the presence and prevalence of Nosemosis in honey bees in the Kırıkkale region and its etiological agent, N. ceranae. In line with the data obtained in this study, it was concluded that Nosemosis should be taken into consideration in bee losses in the region and beekeepers should be made aware of the issue.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"28 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139185072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-08DOI: 10.15312/eurasianjvetsci.2023.416
Meltem Menekse, Atilla Simsek
Aim: It was aimed to reveal the antigenic and serological prevalence of bovine coronavirus (BCoV) in cattle with clinically respiratory infection and to evaluate the relationship between virus shedding and serological status of animals in this study. Materials and Methods: In the study, nasal swab samples were collected from a total of 92 cattle of different gender between 0 and 2 years of age with clinical respiratory symptoms. These samples were subjected to RT-PCR using specific primer pairs. Furthermore, serum samples were also collected from the same animals and evaluated for BCoV-specific antibodies through an indirect ELISA method. Results: Ten (10.87%) swap samples were defined as positive for BCoV using RT-PCR. The seropositivity rate was 93.48% (86/92) in cattle by ELISA and 90% (9/10) in virus-shedding animals with nasal swab samples. Conclusion: The fact that 93.47% seropositivity was detected in unvaccinated animals against coronavirus in this study indicates that the animals were exposed to BCoV through colostrum or at a certain period of their lives. However the fact that 9 out of 10 animals with BCoV detected in swab samples also have BCoV-specific antibodies casts doubt on the efficacy of these antibodies in protecting against respiratory BCoV infections. We concluded that more detailed studies are needed to reveal the role of antibodies in respiratory coronavirus infections.
{"title":"Detection of bovine coronavirus (BCoV) infection in cattle with clinical respiratory signs by PCR and investigation of the serological status of these animals","authors":"Meltem Menekse, Atilla Simsek","doi":"10.15312/eurasianjvetsci.2023.416","DOIUrl":"https://doi.org/10.15312/eurasianjvetsci.2023.416","url":null,"abstract":"Aim: It was aimed to reveal the antigenic and serological prevalence of bovine coronavirus (BCoV) in cattle with clinically respiratory infection and to evaluate the relationship between virus shedding and serological status of animals in this study. Materials and Methods: In the study, nasal swab samples were collected from a total of 92 cattle of different gender between 0 and 2 years of age with clinical respiratory symptoms. These samples were subjected to RT-PCR using specific primer pairs. Furthermore, serum samples were also collected from the same animals and evaluated for BCoV-specific antibodies through an indirect ELISA method. Results: Ten (10.87%) swap samples were defined as positive for BCoV using RT-PCR. The seropositivity rate was 93.48% (86/92) in cattle by ELISA and 90% (9/10) in virus-shedding animals with nasal swab samples. Conclusion: The fact that 93.47% seropositivity was detected in unvaccinated animals against coronavirus in this study indicates that the animals were exposed to BCoV through colostrum or at a certain period of their lives. However the fact that 9 out of 10 animals with BCoV detected in swab samples also have BCoV-specific antibodies casts doubt on the efficacy of these antibodies in protecting against respiratory BCoV infections. We concluded that more detailed studies are needed to reveal the role of antibodies in respiratory coronavirus infections.","PeriodicalId":12023,"journal":{"name":"Eurasian Journal of Veterinary Sciences","volume":"33 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139185022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}