T. I. Assumpção, Adrielly Julien Silva Lopes, L. Fromme, André Luís Quagliatto Santos
Brazil has six species of crocodiles belonging to the subfamily Caimaninae,one of them being Paleosuchus palpebrosus (Cuvier, 1807) (dwarf caiman).It is considered one of the smallest crocodilian species, and is sensitive toenvironmental changes. The anatomical and histological descriptions ofthe reproductive tract of reptiles are scarce, so it is important to study themorphology of the genital tract of caimans for a better understanding of thereproduction of these species. We performed the histological analysis of thetestis, epididymis, and deferent duct of the dwarf caiman Paleosuchus palpebrosus. Structures of the genital tract were collected from euthanized animals. Testes, epididymis, and deferent ducts were separated and preparedaccording to routine histological techniques for morphological characterization. The testis is covered by a capsule of fibromuscular connective tissue.Spermatogonia are observed as spherical cells with round nuclei and loosechromatin, and primary spermatocytes show dark cytoplasm, round nuclei,and pale chromatin. Spermatids are seen as cylindrical or filamentous cellswith nuclei that may be highly condensed, ranging from round to oval. Theepididymis is observed as a coiled duct lined by a tall cuboidal epitheliumwith stereocilia; the deferent duct shows ciliated pseudostratified cylindrical epithelium. The adrenal gland is found in the dorsomedial portion of thetestis. The histological structure of the reproductive tract of Paleosuchuspalpebrosus is similar to that of other reptiles’species.
{"title":"Histological Study of the Male Internal Genital Tract of Dwarf Caiman (Paleosuchus palpebrosus Cuvier, 1807)","authors":"T. I. Assumpção, Adrielly Julien Silva Lopes, L. Fromme, André Luís Quagliatto Santos","doi":"10.30564/jzr.v3i3.3448","DOIUrl":"https://doi.org/10.30564/jzr.v3i3.3448","url":null,"abstract":"Brazil has six species of crocodiles belonging to the subfamily Caimaninae,one of them being Paleosuchus palpebrosus (Cuvier, 1807) (dwarf caiman).It is considered one of the smallest crocodilian species, and is sensitive toenvironmental changes. The anatomical and histological descriptions ofthe reproductive tract of reptiles are scarce, so it is important to study themorphology of the genital tract of caimans for a better understanding of thereproduction of these species. We performed the histological analysis of thetestis, epididymis, and deferent duct of the dwarf caiman Paleosuchus palpebrosus. Structures of the genital tract were collected from euthanized animals. Testes, epididymis, and deferent ducts were separated and preparedaccording to routine histological techniques for morphological characterization. The testis is covered by a capsule of fibromuscular connective tissue.Spermatogonia are observed as spherical cells with round nuclei and loosechromatin, and primary spermatocytes show dark cytoplasm, round nuclei,and pale chromatin. Spermatids are seen as cylindrical or filamentous cellswith nuclei that may be highly condensed, ranging from round to oval. Theepididymis is observed as a coiled duct lined by a tall cuboidal epitheliumwith stereocilia; the deferent duct shows ciliated pseudostratified cylindrical epithelium. The adrenal gland is found in the dorsomedial portion of thetestis. The histological structure of the reproductive tract of Paleosuchuspalpebrosus is similar to that of other reptiles’species.","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88424440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to adapt to the low temperature environment, organisms transmitexcitement to the central system through the thermal sensing system, whichis a classic reflex reaction. The cold receptor GLR-3 perceives cold and produces cold avoidance behavior through peripheral sensory neurons ASER.In order to further understand the gene encoding of the cold sensing glr-3gene and the evolution of its homologous gene group function and proteinfunction, the nucleotide sequence and amino acid sequence of the glr-3gene and its homologous gene in 24 species were obtained and compared.By clustering with the GRIK2 gene sequence of Rana chensinensis, the bioinformatics method was used to predict and sequence analyze the change ofgene, evolution rate, physical and chemical properties of protein, glycosylation sites, phosphorylation sites, secondary structure and tertiary structureof protein. The analysis results show that the glr-3 gene and its homologousgene have obvious positive selection effect. The protein prediction analysisshowed that the glr-3 gene and its homologous genes encoded proteinsin these 25 species were hydrophilic proteins, and the proportion of sidechains of aliphatic amino acids was high. The transmembrane helix waswidespread and there were more N-glycosylation sites and O-glycosylationsites. The protein phosphorylation sites encoded were serine, threonine andtyrosine phosphorylation sites. Secondary structure prediction showed thatthe secondary structure units of the encoded protein were α-helix, β-turn,random coil and extended chain, and the proportion of α-helix was the largest. This study provides useful information on the evolution and function ofthe cold sensing gene glr-3 and its homologous genes.
{"title":"Clustering and Differentiation of glr-3 Gene Function and Its Homologous Proteins","authors":"Yue Ma, Tiantian Guo, Yihe Wang, Xinna Li, Jingyu Zhang","doi":"10.30564/jzr.v3i3.3337","DOIUrl":"https://doi.org/10.30564/jzr.v3i3.3337","url":null,"abstract":"In order to adapt to the low temperature environment, organisms transmitexcitement to the central system through the thermal sensing system, whichis a classic reflex reaction. The cold receptor GLR-3 perceives cold and produces cold avoidance behavior through peripheral sensory neurons ASER.In order to further understand the gene encoding of the cold sensing glr-3gene and the evolution of its homologous gene group function and proteinfunction, the nucleotide sequence and amino acid sequence of the glr-3gene and its homologous gene in 24 species were obtained and compared.By clustering with the GRIK2 gene sequence of Rana chensinensis, the bioinformatics method was used to predict and sequence analyze the change ofgene, evolution rate, physical and chemical properties of protein, glycosylation sites, phosphorylation sites, secondary structure and tertiary structureof protein. The analysis results show that the glr-3 gene and its homologousgene have obvious positive selection effect. The protein prediction analysisshowed that the glr-3 gene and its homologous genes encoded proteinsin these 25 species were hydrophilic proteins, and the proportion of sidechains of aliphatic amino acids was high. The transmembrane helix waswidespread and there were more N-glycosylation sites and O-glycosylationsites. The protein phosphorylation sites encoded were serine, threonine andtyrosine phosphorylation sites. Secondary structure prediction showed thatthe secondary structure units of the encoded protein were α-helix, β-turn,random coil and extended chain, and the proportion of α-helix was the largest. This study provides useful information on the evolution and function ofthe cold sensing gene glr-3 and its homologous genes.","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"829 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77545251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The main animal protein ingredient in fish diets is most often fishmeal because of its nutritional quality. However, the limited availability and increasingly cost of fishmeal has lead to investigations of either lowering or replacing the fishmeal content with more economic protein sources of animal and/or plant origin. The research for suitable and cost-effective alternative protein sources for use in commercial fish diets will be the most critical factor in the development of intensive aquaculture. Insect meals are nutritious and healthy alternatives to fishmeal because of its rich nutritional values especially protein, fat and minerals. Housefly maggot (Musca domestica) meal is also rich in B complex vitamins, trace elements and phosphorus. From the results of previous studies, Housefly maggot meal can be successfully used to replace fishmeal portion partially or completely in the fish diets. Also, the results observed that not physiological stressful was introduced in the fish by feeding Housefly maggot meal diets. This indicates that Housefly maggots meal were well utilized by the fish thus resulting in good growth of fish. In other study, observed a best performance of fish that fed diets containing maggot meal over those solely fed on fishmeal diets. This is a reflection of the nutritional quality and acceptance of this biomaterial.
{"title":"Review on using of housefly maggots (Musca domestica) in fish diets","authors":"H. Saleh","doi":"10.30564/jzr.v2i4.2190","DOIUrl":"https://doi.org/10.30564/jzr.v2i4.2190","url":null,"abstract":" The main animal protein ingredient in fish diets is most often fishmeal because of its nutritional quality. However, the limited availability and increasingly cost of fishmeal has lead to investigations of either lowering or replacing the fishmeal content with more economic protein sources of animal and/or plant origin. The research for suitable and cost-effective alternative protein sources for use in commercial fish diets will be the most critical factor in the development of intensive aquaculture. Insect meals are nutritious and healthy alternatives to fishmeal because of its rich nutritional values especially protein, fat and minerals. Housefly maggot (Musca domestica) meal is also rich in B complex vitamins, trace elements and phosphorus. From the results of previous studies, Housefly maggot meal can be successfully used to replace fishmeal portion partially or completely in the fish diets. Also, the results observed that not physiological stressful was introduced in the fish by feeding Housefly maggot meal diets. This indicates that Housefly maggots meal were well utilized by the fish thus resulting in good growth of fish. In other study, observed a best performance of fish that fed diets containing maggot meal over those solely fed on fishmeal diets. This is a reflection of the nutritional quality and acceptance of this biomaterial. ","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91226811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Saleh, Ahmed E. Semaida, S. Zidan, Ramadan M. Abou-Zied, S. Allam
This study was conducted to investigate the effect of different doses injection of human chorionic gonadotropin (HCG) hormone on fecundity and serum sex hormones (FSH, LH, estrogen (E2), progesterone (P4), testosterone (T)) of African catfish (Clarias gariepinus). African catfish spawners were intermuscularly injected with different doses of HCG (500, 1500, 3000, 6000 IU/kg female), and group is not injected as a control; males were injected at half the female dose. The results showed that, fish group injected by 6000 IU HCG/ kg female had the highest gonadsomatic index, absolute fecundity and relative fecundity, while, the lowest value of absolute fecundity and relative fecundity were recorded with 500 IU HCG/ kg female. The group injected with the highest amount of HCG (6000 IU/ kg female) recorded the lowest value from egg diameter, while the highest egg diameter was observed in 500 IU HCG/ kg female. In females, the group injected with 6000 IU HCG/ kg female reflected the lowest level of FSH and the highest level of LH and the highest level of P4 compared to other treatments. Level of T recorded the highest level with 1500 IU HCG/ kg female. The control group reflected the highest level of FSH and E2, while the control group reflected the lowest level of T and P4 level. In males, serum FSH, LH, P4 and E2 in male groups injected with HCG were relatively higher than those recorded in the control group. The highest level of T was recorded in treatment injected with the highest dose of HCG and decreased in other treatments until recorded the lowest level of T in the control group. It was observed, HCG hormone has successfully and accelerate induced spawning in African catfish (Clarias gariepinus) and increased in reproductive performance with the increase in HCG dosage and as compared to group not injected.
本实验研究了注射不同剂量人绒毛膜促性腺激素(HCG)对非洲鲶鱼(Clarias gariepinus)生殖力和血清性激素(FSH、LH、雌激素(E2)、孕酮(P4)、睾酮(T))的影响。将非洲鲶鱼产卵鱼肌间注射不同剂量的HCG(500、1500、3000、6000 IU/kg雌性)，对照组不注射HCG;雄性注射的剂量是雌性的一半。结果表明，6000 IU HCG/ kg雌鱼的性腺指数、绝对繁殖力和相对繁殖力最高，500 IU HCG/ kg雌鱼的绝对繁殖力和相对繁殖力最低。HCG注射量最高组(6000 IU/ kg)卵径值最低，500 IU HCG/ kg时卵径值最高。在女性中，注射6000 IU HCG/ kg组女性FSH水平最低，LH水平最高，P4水平最高。T水平在1500 IU HCG/ kg时最高。对照组FSH、E2水平最高，对照组T、P4水平最低。在男性中，注射HCG的男性组血清FSH、LH、P4和E2均高于对照组。在注射最高剂量HCG的治疗组中记录到最高水平的T，在其他治疗组中下降，直到对照组记录到最低水平的T。观察到，与未注射HCG的组相比，HCG激素在非洲鲶鱼(Clarias gariepinus)中成功并加速了诱导产卵，并且随着HCG剂量的增加，繁殖性能有所提高。
{"title":"Sex hormones changes in blood and their effect on fecundity of African catfish (Clarias gariepinus Burchell, 1822) after being injected with different doses of human chorionic gonadotropin (HCG) hormone","authors":"H. Saleh, Ahmed E. Semaida, S. Zidan, Ramadan M. Abou-Zied, S. Allam","doi":"10.30564/jzr.v2i4.2189","DOIUrl":"https://doi.org/10.30564/jzr.v2i4.2189","url":null,"abstract":"This study was conducted to investigate the effect of different doses injection of human chorionic gonadotropin (HCG) hormone on fecundity and serum sex hormones (FSH, LH, estrogen (E2), progesterone (P4), testosterone (T)) of African catfish (Clarias gariepinus). African catfish spawners were intermuscularly injected with different doses of HCG (500, 1500, 3000, 6000 IU/kg female), and group is not injected as a control; males were injected at half the female dose. The results showed that, fish group injected by 6000 IU HCG/ kg female had the highest gonadsomatic index, absolute fecundity and relative fecundity, while, the lowest value of absolute fecundity and relative fecundity were recorded with 500 IU HCG/ kg female. The group injected with the highest amount of HCG (6000 IU/ kg female) recorded the lowest value from egg diameter, while the highest egg diameter was observed in 500 IU HCG/ kg female. In females, the group injected with 6000 IU HCG/ kg female reflected the lowest level of FSH and the highest level of LH and the highest level of P4 compared to other treatments. Level of T recorded the highest level with 1500 IU HCG/ kg female. The control group reflected the highest level of FSH and E2, while the control group reflected the lowest level of T and P4 level. In males, serum FSH, LH, P4 and E2 in male groups injected with HCG were relatively higher than those recorded in the control group. The highest level of T was recorded in treatment injected with the highest dose of HCG and decreased in other treatments until recorded the lowest level of T in the control group. It was observed, HCG hormone has successfully and accelerate induced spawning in African catfish (Clarias gariepinus) and increased in reproductive performance with the increase in HCG dosage and as compared to group not injected. ","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78757164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objectives of this study were to: identify the relationship between vegetation higheit and birds’ species at various wetlands of Sinnar State; This study was conducted in Sinnar state, central Sudan (Latitudes 05º - 12º to 05º -14º N and longitudes 32.9º - 35.4º E), covering 12 wetlands (mayas) during the wet and the dry season duration 2011 - 2013; the wetlands are Ronga, Allahmaana, Gladeem, Elban, Rahad Kobri 45, Lawni, Kinnaf Tura 5, Rigaba, Shamiya, Wad elggack, Homrani and Sinnar Dam reservoir.The study focused on abundance of herbaceous vegetation, Parmeters of herbaceous vegetation were determined at 50-m intervals along line transects; these parameters were plant counts and vegetation height in a 1-m circular quadrat. Birds were counted twice a day (morning and evening) in all wetlands with the help of telescopes and binoculars, and species utilizing each site of the wetlands are identified. Excell programme was used for data analysis.Vegetation height is negatively correlated with birds’ species richness in wet seasons of 2011 – 2012 and 2012 – 2013, positively correlated in the dry season when herbaceous vegetation is tall, but negatively correlated when it is short.
{"title":"Relationship between Vegetation Height and Species richness of birds in some wetlands of Sinnar state, Sudan","authors":"M. Hussien","doi":"10.30564/jzr.v2i4.2136","DOIUrl":"https://doi.org/10.30564/jzr.v2i4.2136","url":null,"abstract":"Objectives of this study were to: identify the relationship between vegetation higheit and birds’ species at various wetlands of Sinnar State; This study was conducted in Sinnar state, central Sudan (Latitudes 05º - 12º to 05º -14º N and longitudes 32.9º - 35.4º E), covering 12 wetlands (mayas) during the wet and the dry season duration 2011 - 2013; the wetlands are Ronga, Allahmaana, Gladeem, Elban, Rahad Kobri 45, Lawni, Kinnaf Tura 5, Rigaba, Shamiya, Wad elggack, Homrani and Sinnar Dam reservoir.The study focused on abundance of herbaceous vegetation, Parmeters of herbaceous vegetation were determined at 50-m intervals along line transects; these parameters were plant counts and vegetation height in a 1-m circular quadrat. Birds were counted twice a day (morning and evening) in all wetlands with the help of telescopes and binoculars, and species utilizing each site of the wetlands are identified. Excell programme was used for data analysis.Vegetation height is negatively correlated with birds’ species richness in wet seasons of 2011 – 2012 and 2012 – 2013, positively correlated in the dry season when herbaceous vegetation is tall, but negatively correlated when it is short. ","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77291331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Lado, David Gwolo Phanuel Mogga, Richard Angelo Lado Benjamin
The study was carried out to determine patterns of birds’ species richness, alpha and beta diversities; and abundance in Badingilo national park using a 10 m fixed-radius point count method. A total of 2670 individuals were recorded from 182 points in the park. The highest expected number of species (Jack1 estimator) was observed in the Riverine habitat and least was in the Agriculture and Human settlement habitat type. The total number of species observed in the park was 63; however Jack1 estimator indicated that there were 68 species in the park. The majority of the birds observed during the study were resident species, few migratory and Palaearctic bird species. Few birds observed in the park were abundant. The most abundant species was the village weaver (381 individuals), and the rarest species were black-bellied bustard, barn owl, black scimitar bill and tree pipit (one individual each).
{"title":"Patterns of Species Richness and Abundance in Badingilo National Park in South Sudan","authors":"T. Lado, David Gwolo Phanuel Mogga, Richard Angelo Lado Benjamin","doi":"10.30564/jzr.v1i1.1284","DOIUrl":"https://doi.org/10.30564/jzr.v1i1.1284","url":null,"abstract":"The study was carried out to determine patterns of birds’ species richness, alpha and beta diversities; and abundance in Badingilo national park using a 10 m fixed-radius point count method. A total of 2670 individuals were recorded from 182 points in the park. The highest expected number of species (Jack1 estimator) was observed in the Riverine habitat and least was in the Agriculture and Human settlement habitat type. The total number of species observed in the park was 63; however Jack1 estimator indicated that there were 68 species in the park. The majority of the birds observed during the study were resident species, few migratory and Palaearctic bird species. Few birds observed in the park were abundant. The most abundant species was the village weaver (381 individuals), and the rarest species were black-bellied bustard, barn owl, black scimitar bill and tree pipit (one individual each).","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75538109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qianli C Ma, Wenchao Wu, N. Ye, X. Wang, P. Yan, Heping Pan
Cells are the basic unit of life structure and life activities. Because of the complex micro-environment of cells, the content of components that play a key role is relatively small, so single-cell analysis is extremely challenging. In recent years, single-cell sequencing technology has been developed and matured. Single-cell sequencing can reveal the composition and physiological diversity of cells, and the existing single-cell separation technology, single-cell whole genome amplification technology, single The principles and applications of cell whole transcriptome amplification technology and single cell transcriptome sequencing are summarized and summarized.
{"title":"Progress in Single Cell Sequencing Technology","authors":"Qianli C Ma, Wenchao Wu, N. Ye, X. Wang, P. Yan, Heping Pan","doi":"10.30564/JZR.V1I1.715","DOIUrl":"https://doi.org/10.30564/JZR.V1I1.715","url":null,"abstract":"Cells are the basic unit of life structure and life activities. Because of the complex micro-environment of cells, the content of components that play a key role is relatively small, so single-cell analysis is extremely challenging. In recent years, single-cell sequencing technology has been developed and matured. Single-cell sequencing can reveal the composition and physiological diversity of cells, and the existing single-cell separation technology, single-cell whole genome amplification technology, single The principles and applications of cell whole transcriptome amplification technology and single cell transcriptome sequencing are summarized and summarized.","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80773575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-13DOI: 10.14302/issn.2694-2275.jzr-19-2695
Généviève Nguedia, E. Miégoué, F. Tendonkeng, Mouchili Mama, Camara Sawa, H. Defang, E. Pamo
The intake and in vivo digestibility of Trypsacumlaxum in guinea pigs according to the graded level of spirulina was evaluated in Cameroon. 20 animals aged of 6 months with an average weight of 450 ± 50 g were randomly divided into 4 equivalent groups. Each group received T. laxum and 40g of compound feed containing 0% (TS0), 2% (TS2), 4% (TS4), and 6% (TS6) of spirulina. Feed intake was the different between the left over and the quantity served. The sample of T. laxum and those of each diet as well as feces were collected and analyzed for the apparent digestibility of each nutrient. Animals were weighed at the beginning and at the end of each period of the test to determine the body weight gain. This study showed that the average daily intake of dry matter (DM) for TS0, TS2, TS4 and TS6 was 74.39 ± 2.98, 78.66 ± 3.14, 83.89 ± 4.28 and 77.76 ± 4.40 g/head/day. The highest apparent digestibility coefficients of different nutrients were obtained with animals fed TS6 group while the lowest were observed in TS0.The apparent digestibility coefficient of dry matter (DM), organic matter (OM) and crude protein (CP) of the supplemented group were statistically higher than those of the control diet. Animals’ weight performances were statistically comparable between treatments. Thus, the combination of T. laxum with concentrated feed containing spirulina can be recommended for guinea-pigs, but the level of incorporation may not exceed 4% of its daily ration.
{"title":"Effect of Graded Levels of Spirulina (Arthropsira platensis) on Feed Intake and in vivo Digestibility of Trypsacum laxum in Guinea Pig (Cavia Porcellus L)","authors":"Généviève Nguedia, E. Miégoué, F. Tendonkeng, Mouchili Mama, Camara Sawa, H. Defang, E. Pamo","doi":"10.14302/issn.2694-2275.jzr-19-2695","DOIUrl":"https://doi.org/10.14302/issn.2694-2275.jzr-19-2695","url":null,"abstract":"The intake and in vivo digestibility of Trypsacumlaxum in guinea pigs according to the graded level of spirulina was evaluated in Cameroon. 20 animals aged of 6 months with an average weight of 450 ± 50 g were randomly divided into 4 equivalent groups. Each group received T. laxum and 40g of compound feed containing 0% (TS0), 2% (TS2), 4% (TS4), and 6% (TS6) of spirulina. Feed intake was the different between the left over and the quantity served. The sample of T. laxum and those of each diet as well as feces were collected and analyzed for the apparent digestibility of each nutrient. Animals were weighed at the beginning and at the end of each period of the test to determine the body weight gain. This study showed that the average daily intake of dry matter (DM) for TS0, TS2, TS4 and TS6 was 74.39 ± 2.98, 78.66 ± 3.14, 83.89 ± 4.28 and 77.76 ± 4.40 g/head/day. The highest apparent digestibility coefficients of different nutrients were obtained with animals fed TS6 group while the lowest were observed in TS0.The apparent digestibility coefficient of dry matter (DM), organic matter (OM) and crude protein (CP) of the supplemented group were statistically higher than those of the control diet. Animals’ weight performances were statistically comparable between treatments. Thus, the combination of T. laxum with concentrated feed containing spirulina can be recommended for guinea-pigs, but the level of incorporation may not exceed 4% of its daily ration.","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87741793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Current methods of ruminant ration formulation in Pakistan use foreign-based nutrient availability values. These values may not be optimal for all geographic areas, as variation in environment, agronomic factors, animal species, and diet characteristics may not be considered. The aim of present study was to establish a database of the chemical composition and dry matter degradation parameters of tropical forage commonly fed to ruminants in Pakistan and South Asian countries using Nili-Ravi buffalo and Cholistani cattle at heifer and lactating stages. Six cereal grain and four legume species were grown in 3 locations under standard agronomic conditions and sampled at booting and at 50% flowering stage for cereal and legumes, respectively. Dried and milled feeds were analyzed for chemical composition and in situ dry matter degradation parameters using 1 g samples in bags placed in the rumen of 2 Nili-Ravi buffalo heifers, 2 lactating Nili-Ravi buffaloes, 2 Cholistani heifers, and 2 lactating Cholistani cows. The forage family (cereal vs. legumes), species, and geographic location of growth significantly influenced (P < 0.001) chemical composition and in situ degradation fractions. Animal species and developmental stage showed no effect on degradation fractions (P > 0.05). Legume-by-heifer interactions significantly increased (P < 0.05), and legume-by-lactating cow interaction tended (P = 0.065), to increase the rate of degradation (Kd). The selected forages were degraded to a similar extent independent of animal species or developmental stage, and legumes are degraded at higher rates and to a greater extent than are cereals. A moderately significant relationship between Kd and effective dry matter degradability (DMD) suggests that Kd could be the single most important predictor of forage degradability in the rumen.
{"title":"Dry matter degradation kinetics of selected tropical forage in Nili-Ravi buffalo and Cholistani cows at heifer and lactating stages using NorFor in situ standards","authors":"M. Tahir","doi":"10.30564/JZR.V1I1.151","DOIUrl":"https://doi.org/10.30564/JZR.V1I1.151","url":null,"abstract":"Current methods of ruminant ration formulation in Pakistan use foreign-based nutrient availability values. These values may not be optimal for all geographic areas, as variation in environment, agronomic factors, animal species, and diet characteristics may not be considered. The aim of present study was to establish a database of the chemical composition and dry matter degradation parameters of tropical forage commonly fed to ruminants in Pakistan and South Asian countries using Nili-Ravi buffalo and Cholistani cattle at heifer and lactating stages. Six cereal grain and four legume species were grown in 3 locations under standard agronomic conditions and sampled at booting and at 50% flowering stage for cereal and legumes, respectively. Dried and milled feeds were analyzed for chemical composition and in situ dry matter degradation parameters using 1 g samples in bags placed in the rumen of 2 Nili-Ravi buffalo heifers, 2 lactating Nili-Ravi buffaloes, 2 Cholistani heifers, and 2 lactating Cholistani cows. The forage family (cereal vs. legumes), species, and geographic location of growth significantly influenced (P < 0.001) chemical composition and in situ degradation fractions. Animal species and developmental stage showed no effect on degradation fractions (P > 0.05). Legume-by-heifer interactions significantly increased (P < 0.05), and legume-by-lactating cow interaction tended (P = 0.065), to increase the rate of degradation (Kd). The selected forages were degraded to a similar extent independent of animal species or developmental stage, and legumes are degraded at higher rates and to a greater extent than are cereals. A moderately significant relationship between Kd and effective dry matter degradability (DMD) suggests that Kd could be the single most important predictor of forage degradability in the rumen.","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88871178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present feeding trial, responses of laying hens, kept at high ambient temperature, to various dietary ratios of linoleic acid (LNA) to α-linolenic acid (ALA) and vitamin A levels on performance and egg characteristics were evaluated. A total of 360, 40-wk-old, Leghorn laying hens were fed on diets with various combinations of canola oil and linseed oil to achieve LNA to ALA dietary ratios of 20:1, 10:1, 4:1, 2:1, 1:1 and 1:2, each supplemented with 3000 IU or 10000 IU vitamin A/kg of diet. The diets were fed in a 6×2 factorial Completely Randomized Design that continued for 12 weeks. Feed intake, weight gain, egg production and egg quality traits were recorded during the trial. Decreasing dietary LNA to ALA ratio in the diet affected negatively (P<0.05) on body weight gain and yolk percentage in laying hens. While, feed intake, hen-day and hen-housed egg production, feed conversion ratio (FCR) per dozen of eggs and shell quality remained unaffected (P>0.05) by dietary treatments, with the exception of FCR per kg eggs, egg weight and egg-shell thickness which responded significantly (P<0.05) to various dietary treatments. Although the dietary ratio of LNA to ALA of 4:1 or less could produce eggs by the hens with desirable quantities of n-6 and n-3 poly unsaturated fatty acids – that are characteristics of functional diets – the performance of laying hens in terms of body weight gain and egg-yolk percentage was slightly compromised.
{"title":"Effects of supplementing various linoleic to α-linolenic acid ratios and vitamin A on production performance and egg characteristics of laying hens during summer months","authors":"M. Tahir","doi":"10.30564/jzr.v1i1.112","DOIUrl":"https://doi.org/10.30564/jzr.v1i1.112","url":null,"abstract":"In the present feeding trial, responses of laying hens, kept at high ambient temperature, to various dietary ratios of linoleic acid (LNA) to α-linolenic acid (ALA) and vitamin A levels on performance and egg characteristics were evaluated. A total of 360, 40-wk-old, Leghorn laying hens were fed on diets with various combinations of canola oil and linseed oil to achieve LNA to ALA dietary ratios of 20:1, 10:1, 4:1, 2:1, 1:1 and 1:2, each supplemented with 3000 IU or 10000 IU vitamin A/kg of diet. The diets were fed in a 6×2 factorial Completely Randomized Design that continued for 12 weeks. Feed intake, weight gain, egg production and egg quality traits were recorded during the trial. Decreasing dietary LNA to ALA ratio in the diet affected negatively (P<0.05) on body weight gain and yolk percentage in laying hens. While, feed intake, hen-day and hen-housed egg production, feed conversion ratio (FCR) per dozen of eggs and shell quality remained unaffected (P>0.05) by dietary treatments, with the exception of FCR per kg eggs, egg weight and egg-shell thickness which responded significantly (P<0.05) to various dietary treatments. Although the dietary ratio of LNA to ALA of 4:1 or less could produce eggs by the hens with desirable quantities of n-6 and n-3 poly unsaturated fatty acids – that are characteristics of functional diets – the performance of laying hens in terms of body weight gain and egg-yolk percentage was slightly compromised.","PeriodicalId":12046,"journal":{"name":"European Journal of Zoological Research","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86018211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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