Pub Date : 2020-07-01DOI: 10.25040/ecpb2020.02.044
{"title":"Ontogenetic peculiarities of autonomic regulation of the cardiovascular system of male rats by parameters of heart rate variability","authors":"","doi":"10.25040/ecpb2020.02.044","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.044","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77785962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.25040/ecpb2020.02.068
{"title":"Infrared spectroscopy of blood of cancer patients","authors":"","doi":"10.25040/ecpb2020.02.068","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.068","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85787432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.25040/ecpb2020.02.021
Щорічно у світі реєструється близько 17 млн інсультів, із них – від 100 до 110 тис. серед мешканців України. Інсульт посідає перше місце за наслідками стійкої інвалідизації людей у всьому світі [1,2]. У 80 % пацієнтів після завершення гострого періоду розвивається поліморфна картина порушень рухових функцій: парез, синдроми спастичності та патологічних синергій [1,2]. Відновлення рухових функцій зумовлене активацією первинних моторних представництв навколоінфарктного регіону, тоді як розвиток малоадаптивних моделей рухового контролю пов`язують із комплексним процесом реорганізації, як ураженої, так й неураженої гемісфери [2,3]. Продемонстровано, що регрес парезу корелює із відновленням кортикоспінальної іннервації за даними транскраніальної магнітної стимуляції (ТМС). Водночас розвиток спастичності та патологічних синергій не має прямої залежності зі ступенем порушення кортикоспінальної іннервації. Дані порушення асоціюють зі складною малоадаптивною реорганізацією із залученням вторинних, іпсілатеральних моторних представництв, гіперактивацією контралатеральної півкулі та вивільненням підкоркової активності [3–6]. Як відомо з наукових джерел, для вивчення реорганізації функцій головного мозку застосовується поєднання методів ТМС та електроенцефалографії (ЕЕГ) [4,6,7]. У результаті вивчення змін біоелектричної активності головного мозку продемонстровано зниження активності альфа-ритму та уповільнення ЕЕГ в навколоінфарктному регіоні, що пов’язують з негативним прогнозом відновлення та вивільненням підкоркової активності [8–11]. Підвищення потужності дельта-ритму в інтактній півкулі асоційовано із міжгемісферною дисоціацією [12–14]. Відновлення альфаактивності у центральних регіонах асоційоване з фізіологічною реорганізацією. Збереження бета-активності в ураженій півкулі вказує на патологічну реорганізацію та відсутність відновлення. Зсув когерентності з контралатеральною півкулею та збереження потужності у бета-частотному діапазоні вказує на малоадаптивну реорганізацію [6,15–17]. Враховуючи високий рівень поширеності, тяжкість наслідків інсульту та недостатню вивченість механізмів реорганізації головного мозку, вважаємо актуальним та перспективним питанням вивчення реорганізації функцій
{"title":"Features of the structure of bioelectrical activity of the brain in patients with varying degrees of injury of corticospinal innervation due to hemispheric ischemic stroke","authors":"","doi":"10.25040/ecpb2020.02.021","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.021","url":null,"abstract":"Щорічно у світі реєструється близько 17 млн інсультів, із них – від 100 до 110 тис. серед мешканців України. Інсульт посідає перше місце за наслідками стійкої інвалідизації людей у всьому світі [1,2]. У 80 % пацієнтів після завершення гострого періоду розвивається поліморфна картина порушень рухових функцій: парез, синдроми спастичності та патологічних синергій [1,2]. Відновлення рухових функцій зумовлене активацією первинних моторних представництв навколоінфарктного регіону, тоді як розвиток малоадаптивних моделей рухового контролю пов`язують із комплексним процесом реорганізації, як ураженої, так й неураженої гемісфери [2,3]. Продемонстровано, що регрес парезу корелює із відновленням кортикоспінальної іннервації за даними транскраніальної магнітної стимуляції (ТМС). Водночас розвиток спастичності та патологічних синергій не має прямої залежності зі ступенем порушення кортикоспінальної іннервації. Дані порушення асоціюють зі складною малоадаптивною реорганізацією із залученням вторинних, іпсілатеральних моторних представництв, гіперактивацією контралатеральної півкулі та вивільненням підкоркової активності [3–6]. Як відомо з наукових джерел, для вивчення реорганізації функцій головного мозку застосовується поєднання методів ТМС та електроенцефалографії (ЕЕГ) [4,6,7]. У результаті вивчення змін біоелектричної активності головного мозку продемонстровано зниження активності альфа-ритму та уповільнення ЕЕГ в навколоінфарктному регіоні, що пов’язують з негативним прогнозом відновлення та вивільненням підкоркової активності [8–11]. Підвищення потужності дельта-ритму в інтактній півкулі асоційовано із міжгемісферною дисоціацією [12–14]. Відновлення альфаактивності у центральних регіонах асоційоване з фізіологічною реорганізацією. Збереження бета-активності в ураженій півкулі вказує на патологічну реорганізацію та відсутність відновлення. Зсув когерентності з контралатеральною півкулею та збереження потужності у бета-частотному діапазоні вказує на малоадаптивну реорганізацію [6,15–17]. Враховуючи високий рівень поширеності, тяжкість наслідків інсульту та недостатню вивченість механізмів реорганізації головного мозку, вважаємо актуальним та перспективним питанням вивчення реорганізації функцій","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75414440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-01DOI: 10.25040/ecpb2020.01.036
{"title":"The aminoguanidine effect on the content of cerebellar glial fibrillary acidic protein in experimental antiphospholipid syndrome with pregnancy","authors":"","doi":"10.25040/ecpb2020.01.036","DOIUrl":"https://doi.org/10.25040/ecpb2020.01.036","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84197642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-01DOI: 10.25040/ecpb2020.01.015
I. Kovalenko, O. K. Onufrovych, R. Fafula, O. Melnyk, Z. Vorobets, Danylo Halytskyi
Antiseptic drugs have mostly microbicidal effect on the skin surface, mucous membranes, surfaces of a wound and body cavities. Decamethoxine is a chemical compound [1, 10-decameth-ylene bis (N, N – dimethylmethoxycarbonylmethyl) ammonium dichloride] that has a wide range of antimicrobial actions on Gram - positive (staphylococcus, streptococcus, pneumococcus), Gram-negative (gonococcus, meningococcus) cocci, diphtheria corynebacteria, enterobacteria, pseudomonads, protozoa, dermatophytes, yeasts-like fungi of the genus Candida , chlamydia and viruses. Pathological processes that occur in the cells of the body when inflammatory processes and pathological conditions are caused primarily by the violation of regulatory systems, the activation of lipid peroxidation processes and the violation of antioxidant protective system. Since decamethoxine has hydrophilic and lipophilic properties, it can penetrate into cells, blood through surfaces of a wound, mucous membranes, skin, etc., and be transmitted by blood to different organs and tissues, thus causing a variety of biochemical effects, particularly regarding to the regulatory NO-synthase system of cells. In this respect the biological action of decamethoxine nisms of the cell, blood lymphocytes in particular. It activates the enzymes of the glutathione antioxidant system, stimulates the activity of arginase and inhibits the activity of the constitutive isoform of NO synthase with the simultaneous activation of the inducible isoform of NO synthase.
抗菌药物主要对皮肤表面、粘膜、伤口表面和体腔有杀微生物作用。十甲氧嘧啶是一种化合物[1,10 -十甲氧基二(N, N -二甲基甲氧基羰基甲基)二氯化铵]，对革兰氏阳性(葡萄球菌、链球菌、肺炎球菌)、革兰氏阴性(淋球菌、脑膜炎球菌)球菌、白喉粘连菌、肠细菌、假单胞菌、原生动物、皮肤真菌、酵母菌属类酵母菌、衣原体和病毒具有广泛的抗菌作用。当炎症过程和病理状况主要由调节系统的破坏、脂质过氧化过程的激活和抗氧化保护系统的破坏引起时，发生在身体细胞中的病理过程。由于十甲氧胺具有亲水性和亲脂性，可通过创面、粘膜、皮肤等渗透到细胞、血液中，并经血液传递到不同的器官和组织，从而引起多种生化作用，特别是对细胞的调节性no -合成酶系统。在这方面，十甲氧苄啶对细胞的生物学作用，特别是血液淋巴细胞。它激活谷胱甘肽抗氧化系统的酶，刺激精氨酸酶的活性，抑制NO合成酶的组成异构体的活性，同时激活NO合成酶的诱导异构体。
{"title":"Characteristics of antioxidant and NO-synthase systems of blood lymphocytes under the action of decamethoxine","authors":"I. Kovalenko, O. K. Onufrovych, R. Fafula, O. Melnyk, Z. Vorobets, Danylo Halytskyi","doi":"10.25040/ecpb2020.01.015","DOIUrl":"https://doi.org/10.25040/ecpb2020.01.015","url":null,"abstract":"Antiseptic drugs have mostly microbicidal effect on the skin surface, mucous membranes, surfaces of a wound and body cavities. Decamethoxine is a chemical compound [1, 10-decameth-ylene bis (N, N – dimethylmethoxycarbonylmethyl) ammonium dichloride] that has a wide range of antimicrobial actions on Gram - positive (staphylococcus, streptococcus, pneumococcus), Gram-negative (gonococcus, meningococcus) cocci, diphtheria corynebacteria, enterobacteria, pseudomonads, protozoa, dermatophytes, yeasts-like fungi of the genus Candida , chlamydia and viruses. Pathological processes that occur in the cells of the body when inflammatory processes and pathological conditions are caused primarily by the violation of regulatory systems, the activation of lipid peroxidation processes and the violation of antioxidant protective system. Since decamethoxine has hydrophilic and lipophilic properties, it can penetrate into cells, blood through surfaces of a wound, mucous membranes, skin, etc., and be transmitted by blood to different organs and tissues, thus causing a variety of biochemical effects, particularly regarding to the regulatory NO-synthase system of cells. In this respect the biological action of decamethoxine nisms of the cell, blood lymphocytes in particular. It activates the enzymes of the glutathione antioxidant system, stimulates the activity of arginase and inhibits the activity of the constitutive isoform of NO synthase with the simultaneous activation of the inducible isoform of NO synthase.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81010878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-01DOI: 10.25040/ecpb2020.01.023
{"title":"The bioelectric activity of the efferent sciatic nerve fibers in conditions of experimental hypoandrogenemy","authors":"","doi":"10.25040/ecpb2020.01.023","DOIUrl":"https://doi.org/10.25040/ecpb2020.01.023","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75616764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-01DOI: 10.25040/ecpb2020.01.050
An important component of therapeutic measures for mucoviscidose is the determination of the functional state of the respiratory system, the fight against an opportunistic infection which accompanies the course of the chronic microbial-inflammatory process in the bronchopulmonary tract. Under conditions of reduced resistance to the child’s 9 patients (47,4 %) – fungi of the genus Candida. In 2 patients (10,5 %) Escherichia with atypical properties was found. Hemolytic E. coli were detected in one of the patients. The pathogenic enterobacteria and staphylococcus were not detected in the feces of any patient. 4 patients (21 %) were isolated because of S. coseri, C. freundii and K.pneumonia. In 2 patients (10,5 %) from the group “first diagnosed mucoviscidose” the state of the intestinal microflora was almost within the normal range. In 17 patient (89,5 %) postmedical dysbiosis was indicated. According to the results of microbiological studies, the endogenous colonization of the intestines of patients with mucoviscidose sputum has not been confirmed. One hundred percent of patients had steatorrhea; two patients had steatorrhea combined with creatorrhea.
{"title":"The physiological and microbiological characteristics of bronchopulmonary and intestinal tracts in children with cystic fibrosis","authors":"","doi":"10.25040/ecpb2020.01.050","DOIUrl":"https://doi.org/10.25040/ecpb2020.01.050","url":null,"abstract":"An important component of therapeutic measures for mucoviscidose is the determination of the functional state of the respiratory system, the fight against an opportunistic infection which accompanies the course of the chronic microbial-inflammatory process in the bronchopulmonary tract. Under conditions of reduced resistance to the child’s 9 patients (47,4 %) – fungi of the genus Candida. In 2 patients (10,5 %) Escherichia with atypical properties was found. Hemolytic E. coli were detected in one of the patients. The pathogenic enterobacteria and staphylococcus were not detected in the feces of any patient. 4 patients (21 %) were isolated because of S. coseri, C. freundii and K.pneumonia. In 2 patients (10,5 %) from the group “first diagnosed mucoviscidose” the state of the intestinal microflora was almost within the normal range. In 17 patient (89,5 %) postmedical dysbiosis was indicated. According to the results of microbiological studies, the endogenous colonization of the intestines of patients with mucoviscidose sputum has not been confirmed. One hundred percent of patients had steatorrhea; two patients had steatorrhea combined with creatorrhea.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77070975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-01DOI: 10.25040/ecpb2020.01.058
{"title":"Realization of new approaches in treatment and care The realization of new approaches in treatment and nurse care of pancreatic diseases","authors":"","doi":"10.25040/ecpb2020.01.058","DOIUrl":"https://doi.org/10.25040/ecpb2020.01.058","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"16 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88923310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-01DOI: 10.25040/ecpb2020.01.066
The article describes the psychophysiological features of people with the increased sensitivity to the vestibular analyzer. The urgency is due to the fact that the vestibular analyzer provides a sense of position, movement of a human body and its parts in space, maintains a pose in all possible types of human activity, performs the function of the body equilibrium both at rest and during the process of movement. The question about the role of the vestibular system in compensation for the linear and angular accelerations that occur during arbitrary or unexpected movements has been investigated for many decades. The increased sensitivity of the vestibular analyzer does not at first glance cause an immediate threat to a humam life, however, it degrades its quality significantly. However, it has a progressive nature and is accompanied by insufficient compensation under stress. The reactions of the organism with increased sensitivity to the vestibular analyzer are investigated in the studies, aimed mainly at identifying the professional features of functioning tation mechanisms. The functional status of these individuals is impaired as a result of reduced functional and metabolic reserves of a body, especially in the III type of weather. The obtained data also correlate neuroticism with many important physiological indicators. The data described in the article add more to the understanding of the features of the body response to the vestibular load in people with increased sensitivity to the vestibular analyzer and can be used as a prognostic criterion during prophylactic medical examinations and in choosing a career.
{"title":"Some psychophysiological features of people with hypersensitivity to the vestibular analyzer","authors":"","doi":"10.25040/ecpb2020.01.066","DOIUrl":"https://doi.org/10.25040/ecpb2020.01.066","url":null,"abstract":"The article describes the psychophysiological features of people with the increased sensitivity to the vestibular analyzer. The urgency is due to the fact that the vestibular analyzer provides a sense of position, movement of a human body and its parts in space, maintains a pose in all possible types of human activity, performs the function of the body equilibrium both at rest and during the process of movement. The question about the role of the vestibular system in compensation for the linear and angular accelerations that occur during arbitrary or unexpected movements has been investigated for many decades. The increased sensitivity of the vestibular analyzer does not at first glance cause an immediate threat to a humam life, however, it degrades its quality significantly. However, it has a progressive nature and is accompanied by insufficient compensation under stress. The reactions of the organism with increased sensitivity to the vestibular analyzer are investigated in the studies, aimed mainly at identifying the professional features of functioning tation mechanisms. The functional status of these individuals is impaired as a result of reduced functional and metabolic reserves of a body, especially in the III type of weather. The obtained data also correlate neuroticism with many important physiological indicators. The data described in the article add more to the understanding of the features of the body response to the vestibular load in people with increased sensitivity to the vestibular analyzer and can be used as a prognostic criterion during prophylactic medical examinations and in choosing a career.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74028276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-01DOI: 10.25040/ecpb2020.01.044
The Na + , K + -ATPase is an ubiquitously expressed P-type of ATPase. Its main role is to maintain Na + and K + gradients across the plasma membrane by ATP-driven active transport. The Na + , K + -ATPase was one of the first ion pumps studied because of its importance in main-taining osmotic and ionic balances between intracellular and extracellular environments. Ad-ditional extracellular stimuli have been shown to induce internalization of the Na in the incubation medium, including the loss of endogenous phosphorus Pi in the membrane preparation, expressed in μmoles Pi per minutes per milligram of protein. The amount of endogenous phosphorus has been identified by the modified Fiske-Subbarow method, the quanti-tation of protein in the membrane preparation has been determined by Lowry method and the activation energy – in the Arrhenius coordinates. The activity of ouabain-sensitive ATPase under the change in the incubation medium temperature gradually increased at the stages of embryos development by an average of 23.4 ± 1.6% as compared with the control group. The maximum value of the enzymatic activity of Na + , K + pump of the embryos has been defined at the 8-stage of blastomers division (270 min). At the 6th hour of the development, no significant changes in the activity of Na + , K + -ATPase has been observed in comparison with the previous stage of the development. The results have shown that with the temperature change (10 o C) of the incubation medium, the Na + , K + -ATPase activity increased significantly at the investigated stages of the development. For Na + , K + -ATPase, a nonlinear dependence of the enzymatic activity on the temperature in the Arrhenius coordinates was found; the activation energy is 10.6 ÷ 20.8 kJ / mol. The calculated values of the Na + , K + -ATPase activation energy of the loach embryos during embryogenesis are consistent with the existing data and are likely due to the binding strength of the investigated ATP-hydrolase to membrane lipids during the embryonal cell division.
Na +， K + - atp酶是一种普遍表达的p型atp酶。其主要作用是通过atp驱动的主动转运维持Na +和K +在质膜上的梯度。Na +， K + - atp酶是最早被研究的离子泵之一，因为它在维持细胞内和细胞外环境之间的渗透和离子平衡方面具有重要意义。额外的细胞外刺激已被证明可以诱导培养液中Na的内化，包括在膜制备中内源性磷Pi的损失，以μ摩尔Pi每分钟每毫克蛋白质表示。用改进的Fiske-Subbarow法测定了内源磷的量，用Lowry法测定了膜制备过程中蛋白质的定量，并在Arrhenius坐标系下测定了活化能。在胚胎发育阶段，随着培养液温度的变化，瓦巴因敏感atp酶的活性逐渐升高，平均较对照组升高23.4±1.6%。胚胎的Na +、K +泵酶活性在囊胚分裂第8期(270 min)达到最大值。在发育第6小时，Na +， K + - atp酶的活性与发育前阶段相比没有明显变化。结果表明，随着培养温度(10℃)的变化，Na +， K + - atp酶活性在发育阶段显著升高。对于Na +， K + - atp酶，在Arrhenius座标下，酶活性与温度呈非线性关系;泥鳅胚胎在胚胎发生过程中Na +、K + - atp酶的活化能计算值与已有数据一致，可能与胚胎细胞分裂过程中所研究的atp水解酶与膜脂的结合强度有关。
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