Pub Date : 2019-11-10DOI: 10.25040/ecpb2019.03.056
N. Sheiko, V. Feketa, K. Kivezhdi
Autonomic dysfunction syndrome is a polyetiological syndrome characterized by dysfunction of the autonomic nervous system and functional (inorganic) disorders by all body systems, the prevalence of which ranges from 20 to 56% of all diseases in young people. Most researchers consider heart rate variability as an integral marker that reflects the state of all the body's regulatory systems and is now considered an affordable, non-invasive, reasonably simple and relatively inexpensive method for evaluating the autonomic nervous system. The ability to correct autonomic dysfunction through diaphragmatic breathing in biological feedback using portable computer devices has been demonstrated. However, there is little scientific evidence on the use of breathing exercises in yoga to influence the functional state of the autonomic nervous system. The aim of the study was to investigate the possibility of using “pranayama” breathing exercises to correct autonomic dysfunction in young adults. The study involved 30 healthy young foreigners aged 18 to 24 years. Heart rate variability was recorded using the CardioLab Computer Diagnostic Complex (KhAI-Medica, Ukraine) in the supine position after 10 minutes of adaptation to the registration conditions. The last 5 min utes of registration were used as background metrics. The distribution of the examined into the dependent groups was performed according to the indicators of the output tone of the activity of autonomic system’s regulation. Subsequently, for a period of 30 days, a 15-minute session of respiratory gymnastics was conducted daily, which consisted of 3 periods of 5 minutes, during which the subject performed 5-6 breathing cycles per minute. Each period was completed with 3 spontaneous breathing cycles. The obtained numerical data were processed by the methods of variational statistics using Student's test. The dynamics of heart rate variability was assessed by pairwise comparisons, and intergroup differences were determined using one-way ANOVA. Under the influence of a 30-day course of breathing gymnastics "pranayama" indicators of the functional state of the autonomic nervous system have undergone significant changes, in particular in groups of people with initial sympathicotonia. The overall heart rate variability according to TP at the end of the course remained higher than the background values, respec -tively, by 697±101ms2 (p<0.05) in the sympathotonic group and by 398 ± 95ms 2 (p<0,05) in the parasympathicotonic group. Changes in TP in the sympathotonic group occurred mainly due to a decrease in the contribution of the low-frequency (VLF) heart rate regulation (by 98 ± 35ms2 (p <0,05) and a significant increase in the contribution of the high-frequency component of HF (by 682±72 ms2 (p<0,05) There were also significant changes in the structure of the heart rate spectrum according to the percentage contribution of waves of different frequencies at TP. At the end of the course in the sympathotonic g
{"title":"Correction of Autonomous Dysfunctions by the Prayama Respiratory Gymnastics in Young People","authors":"N. Sheiko, V. Feketa, K. Kivezhdi","doi":"10.25040/ecpb2019.03.056","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.056","url":null,"abstract":"Autonomic dysfunction syndrome is a polyetiological syndrome characterized by dysfunction of the autonomic nervous system and functional (inorganic) disorders by all body systems, the prevalence of which ranges from 20 to 56% of all diseases in young people. Most researchers consider heart rate variability as an integral marker that reflects the state of all the body's regulatory systems and is now considered an affordable, non-invasive, reasonably simple and relatively inexpensive method for evaluating the autonomic nervous system. The ability to correct autonomic dysfunction through diaphragmatic breathing in biological feedback using portable computer devices has been demonstrated. However, there is little scientific evidence on the use of breathing exercises in yoga to influence the functional state of the autonomic nervous system. The aim of the study was to investigate the possibility of using “pranayama” breathing exercises to correct autonomic dysfunction in young adults. The study involved 30 healthy young foreigners aged 18 to 24 years. Heart rate variability was recorded using the CardioLab Computer Diagnostic Complex (KhAI-Medica, Ukraine) in the supine position after 10 minutes of adaptation to the registration conditions. The last 5 min utes of registration were used as background metrics. The distribution of the examined into the dependent groups was performed according to the indicators of the output tone of the activity of autonomic system’s regulation. Subsequently, for a period of 30 days, a 15-minute session of respiratory gymnastics was conducted daily, which consisted of 3 periods of 5 minutes, during which the subject performed 5-6 breathing cycles per minute. Each period was completed with 3 spontaneous breathing cycles. The obtained numerical data were processed by the methods of variational statistics using Student's test. The dynamics of heart rate variability was assessed by pairwise comparisons, and intergroup differences were determined using one-way ANOVA. Under the influence of a 30-day course of breathing gymnastics \"pranayama\" indicators of the functional state of the autonomic nervous system have undergone significant changes, in particular in groups of people with initial sympathicotonia. The overall heart rate variability according to TP at the end of the course remained higher than the background values, respec -tively, by 697±101ms2 (p<0.05) in the sympathotonic group and by 398 ± 95ms 2 (p<0,05) in the parasympathicotonic group. Changes in TP in the sympathotonic group occurred mainly due to a decrease in the contribution of the low-frequency (VLF) heart rate regulation (by 98 ± 35ms2 (p <0,05) and a significant increase in the contribution of the high-frequency component of HF (by 682±72 ms2 (p<0,05) There were also significant changes in the structure of the heart rate spectrum according to the percentage contribution of waves of different frequencies at TP. At the end of the course in the sympathotonic g","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78586708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-10DOI: 10.25040/ecpb2019.03.051
V. Prokopiuk, V. Trifonov, O. Prokopiuk, R. Safonov, V. Lazurenko
Mesenchymal stem cells (MSCs) are actively used in regenerative medicine to treat neuropa -thies, diabetes mellitus, burns, ulcers, infertility, premature conditions are able to maintain metabolism at a level sufficient for survival. The morphological study of cells in alginate microspheres was not informative, because of the difficulty cells visualizing in a three-dimensional structure and the density of alginate. Rapid screening tests may include trypan blue staining and MTT test. Conclusions. Placental cells in alginate microsphers can be stored in subnormothermic (+20°C) conditions no more than 48 hours. Storage more than 48 hours in subnormothermic conditions, or 24 hours in hypothermic conditions leads to a sharp metabolic activity decreasing.
{"title":"Evaluation of the possibility of hypothermic and subornorthomeric storage of placental mesenchymal stem cells in alginate carriers","authors":"V. Prokopiuk, V. Trifonov, O. Prokopiuk, R. Safonov, V. Lazurenko","doi":"10.25040/ecpb2019.03.051","DOIUrl":"https://doi.org/10.25040/ecpb2019.03.051","url":null,"abstract":"Mesenchymal stem cells (MSCs) are actively used in regenerative medicine to treat neuropa -thies, diabetes mellitus, burns, ulcers, infertility, premature conditions are able to maintain metabolism at a level sufficient for survival. The morphological study of cells in alginate microspheres was not informative, because of the difficulty cells visualizing in a three-dimensional structure and the density of alginate. Rapid screening tests may include trypan blue staining and MTT test. Conclusions. Placental cells in alginate microsphers can be stored in subnormothermic (+20°C) conditions no more than 48 hours. Storage more than 48 hours in subnormothermic conditions, or 24 hours in hypothermic conditions leads to a sharp metabolic activity decreasing.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73574185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-05DOI: 10.25040/ECPB2019.02.005
M. Gzhegotskyi, N. Sukhodolska
The global environmental pollution by heavy metals and the imbalance of trace elements has some detrimental effect on pregnancy, fetal development, maternal and newborn’s health with further long-term consequences [1–4]. Heavy metals, particularly lead and cadmium, which are classified as global environmental pollutants, adversely affect the generative function even in low concentrations. Lead excess can cause miscarriage, intrauterine growth retardation, chronic fetal hypoxia, bleeding during pregnancy and labor and the occurrence of birth defects in infants [5,6]. The elevated lead level in pregnant woman’s blood leads to the higher content of this metal in the blood of a newborn child that can cause disorders of the psychological development and child’s mental abilities [6]. The experimental study has shown that the inhalation of cadmium can cause fewer offsprings and higher frequency of congenital malformations, namely embryotoxic and teratogenic effects and in adults – liver damage and alterations in the immune system [7]. The high levels of placental cadmium can result in the reduction of neonatal anthropometric data (weight, height, chest circumference) [8]. Copper as a trace element plays an important in the maturation of hematopoietic cells, in normal pregnancy and embryogenesis, fetal and post-natal growth. Lower plasma copper levels have been found in some conditions diagnosed during the first trimester including a spontaneous, threatened and missed abortion and a blighted ovum [9]. Copper deficiency can result in anemia, neutropenia, bone marrow dysplasia, chronic intrauterine hypoxia, fetal hypotrophy, coagulation abnormalities [9,10]. Serum and placental copper levels and placental lipid peroxides, a marker of oxidative stress, were increased in women with a severe preeclampsia [11]. Zinc deficiency during the pregnancy is associated with preeclampsia, a threatened abortion, a premature birth syndrome, a low birth weight, birth defects, circulatory disorders, impairment of the immune response and disorders of the psychological development [12–14]. Thus, modern ecological situation creates preconditions for increasing the frequency of gestational complications and some negative impact on women and children’s health. In this regard, a scientific search for risk factors of a potential adverse effect on the gestation process for timely implementation of adequate preventive and corrective measures is an important issue. The aim of this study was to evaluate lead, cadmium, copper and zinc levels in women’s blood during II trimester of gestation and to assess the significance of their influence on the gestational process.
{"title":"Assessment of Lead, Cadmium, Copper and Zinc Content in Women’s Blood During II Trimester of Gestation","authors":"M. Gzhegotskyi, N. Sukhodolska","doi":"10.25040/ECPB2019.02.005","DOIUrl":"https://doi.org/10.25040/ECPB2019.02.005","url":null,"abstract":"The global environmental pollution by heavy metals and the imbalance of trace elements has some detrimental effect on pregnancy, fetal development, maternal and newborn’s health with further long-term consequences [1–4]. Heavy metals, particularly lead and cadmium, which are classified as global environmental pollutants, adversely affect the generative function even in low concentrations. Lead excess can cause miscarriage, intrauterine growth retardation, chronic fetal hypoxia, bleeding during pregnancy and labor and the occurrence of birth defects in infants [5,6]. The elevated lead level in pregnant woman’s blood leads to the higher content of this metal in the blood of a newborn child that can cause disorders of the psychological development and child’s mental abilities [6]. The experimental study has shown that the inhalation of cadmium can cause fewer offsprings and higher frequency of congenital malformations, namely embryotoxic and teratogenic effects and in adults – liver damage and alterations in the immune system [7]. The high levels of placental cadmium can result in the reduction of neonatal anthropometric data (weight, height, chest circumference) [8]. Copper as a trace element plays an important in the maturation of hematopoietic cells, in normal pregnancy and embryogenesis, fetal and post-natal growth. Lower plasma copper levels have been found in some conditions diagnosed during the first trimester including a spontaneous, threatened and missed abortion and a blighted ovum [9]. Copper deficiency can result in anemia, neutropenia, bone marrow dysplasia, chronic intrauterine hypoxia, fetal hypotrophy, coagulation abnormalities [9,10]. Serum and placental copper levels and placental lipid peroxides, a marker of oxidative stress, were increased in women with a severe preeclampsia [11]. Zinc deficiency during the pregnancy is associated with preeclampsia, a threatened abortion, a premature birth syndrome, a low birth weight, birth defects, circulatory disorders, impairment of the immune response and disorders of the psychological development [12–14]. Thus, modern ecological situation creates preconditions for increasing the frequency of gestational complications and some negative impact on women and children’s health. In this regard, a scientific search for risk factors of a potential adverse effect on the gestation process for timely implementation of adequate preventive and corrective measures is an important issue. The aim of this study was to evaluate lead, cadmium, copper and zinc levels in women’s blood during II trimester of gestation and to assess the significance of their influence on the gestational process.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75503262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-05DOI: 10.25040/ECPB2019.02.031
A. Ocheretniuk, B. Kondratskyi, O. Palamarchuk, V. Kovalchuk, Y. Kondratskyi
{"title":"Ultrastructural Changes in the Respiratory Section of Rats’ lungs with a Colloid-hyperosmolar Solution Application","authors":"A. Ocheretniuk, B. Kondratskyi, O. Palamarchuk, V. Kovalchuk, Y. Kondratskyi","doi":"10.25040/ECPB2019.02.031","DOIUrl":"https://doi.org/10.25040/ECPB2019.02.031","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73162005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-05DOI: 10.25040/ECPB2019.02.077
M. Ghzehotskyi, Y. Fedorenko
{"title":"БОРИС МИХАЙЛОВИЧ ШТАБСЬКИЙ (до 90-річчя від дня народження (1929–2017)","authors":"M. Ghzehotskyi, Y. Fedorenko","doi":"10.25040/ECPB2019.02.077","DOIUrl":"https://doi.org/10.25040/ECPB2019.02.077","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"218 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75614148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-05DOI: 10.25040/ECPB2019.02.024
M. Kondro, S. Veselskyi, T. Dovbynchuk, I. Prybytko
The effect of a high-calorie diet on the dynamics of choleresis and secretion of free and conjugated fatty acids was studied in experiments on rats. They has shown that in comparison with rats fed with the standard food, in rats that were on a high-calorie diet for 20 weeks, the volume rate of bile secretion increased by 12.4 % (p < 0.05). Using the method of thin layer chromatography, it has been found out that in the bile of rats within a 3-hour experiment the concentration of taurocholic acid mixture of taurochenodeoxycholic and taurodeoxycholic acids, the concentration of the mixture of glycochenodeoxycholic and glycodeoxycholic acids, the concentration of the mixture of chenodeoxycholic and deoxycholic acids increased. At the same time, the concentration of glycocholic acid in the bile of rats decreased after 20 weeks of rats’ staying on HCD, the concentration of cholic acid did not undergo statistically significant changes. In this case, the conjugation coefficient remains unchanged and the hydroxylation coefficient has decreased which indicates the activation of some alternative pathway of biosynthesis of bile acids. The incidence of nonalcoholic steatosis, also called as a nonalcoholic fatty liver disease, is growing rapidly around the world. The growth rate is 30-40 %, which is becoming a huge medical and economic burden for patients and states in the whole [1]. Therefore, the establishment of mechanisms of pathogenesis of steatohepatosis, its consequences and the development of optimal methods of treatment is an urgent problem of our time. Previously, we have shown that the presence of rats on a high-calorie diet No. 11024 (HCD) (diet No. 11024, Research Diets, New Brunswick, NJ) for 20 weeks leads to the development of metabolic syndrome (MS), on the background of which liver steatosis was recorded [2,3]. On the background of structural changes in the liver, there is a change in its functional state, for the assessment of which it is important to determine the spectrum of bile acids in the bile and to establish a ratio between individual bile acids. Under physiological conditions, this spectrum is quite stable. However, in case of impairments of the hepatobiliary system, the content of individual bile acids can vary significantly. Despite the fact that HCD No. 11024 is widely used in experimental studies, the aim of this work was to investigate the range of bile acids cholates in the bile of rats after 20 weeks of their staying on this diet. Materials and methods. Studies were carried out on male white rats with an initial mass of 210-215 grams. During the first week all rats received the standard food and water ad libitum. On the 2nd week the animals were randomized into two groups. Rats of group 1 received the standard food and water ad libitum and served as a control group. Rats of group II were on the HCD (diet No. 11024, Research Diets, New Brunswick, NJ), which consisted
在大鼠实验中研究了高热量饮食对胆汁分泌和游离脂肪酸和共轭脂肪酸分泌的影响。他们发现，与喂食标准食物的老鼠相比，喂食高热量食物20周的老鼠，胆汁分泌的体积率增加了12.4% (p < 0.05)。利用薄层色谱法发现，在实验3小时内，大鼠胆汁中牛磺酰去氧胆酸与牛磺酰去氧胆酸混合物的浓度、糖磺酰去氧胆酸与糖磺酰去氧胆酸混合物的浓度、鹅磺酰去氧胆酸与去氧胆酸混合物的浓度升高。同时，在HCD持续20周后，大鼠胆汁中胆酸浓度下降，胆酸浓度无统计学意义变化。在这种情况下，偶联系数保持不变，羟基化系数降低，表明激活了胆汁酸生物合成的某些替代途径。非酒精性脂肪变性，也称为非酒精性脂肪肝，在世界范围内的发病率正在迅速增长。增长率为30- 40%，整体上成为患者和国家巨大的医疗和经济负担[1]。因此，建立脂肪性肝病的发病机制、其后果和发展最佳的治疗方法是我们这个时代迫切需要解决的问题。在此之前，我们已经证明，高热量饮食No. 11024 (HCD)(饮食No. 11024, Research Diets, New Brunswick, NJ)的大鼠持续20周会导致代谢综合征(MS)的发展，在此背景下记录了肝脏脂肪变性[2,3]。在肝脏结构变化的背景下，肝脏的功能状态也发生了变化，为了评估这种变化，确定胆汁中胆汁酸的光谱并建立各个胆汁酸之间的比例是很重要的。在生理条件下，这个光谱是相当稳定的。然而，在肝胆系统受损的情况下，个体胆汁酸的含量可以显著变化。尽管HCD No. 11024在实验研究中被广泛使用，但本研究的目的是研究大鼠在食用该饮食20周后胆汁中胆汁酸胆酸盐的范围。材料和方法。研究对象是初始质量为210-215克的雄性白鼠。在第一周，所有的大鼠都得到了标准的食物和水。第2周随机分为两组。第1组大鼠给予标准食物和水，随意饲喂，并作为对照组。II组大鼠饲喂HCD(日粮编号11024,Research diet, New Brunswick, NJ)
{"title":"The Range of Bile Acid Cholates in the Bile of Rats With Steatohepatosis Induced by a High-calorie Diet","authors":"M. Kondro, S. Veselskyi, T. Dovbynchuk, I. Prybytko","doi":"10.25040/ECPB2019.02.024","DOIUrl":"https://doi.org/10.25040/ECPB2019.02.024","url":null,"abstract":"The effect of a high-calorie diet on the dynamics of choleresis and secretion of free and conjugated fatty acids was studied in experiments on rats. They has shown that in comparison with rats fed with the standard food, in rats that were on a high-calorie diet for 20 weeks, the volume rate of bile secretion increased by 12.4 % (p < 0.05). Using the method of thin layer chromatography, it has been found out that in the bile of rats within a 3-hour experiment the concentration of taurocholic acid mixture of taurochenodeoxycholic and taurodeoxycholic acids, the concentration of the mixture of glycochenodeoxycholic and glycodeoxycholic acids, the concentration of the mixture of chenodeoxycholic and deoxycholic acids increased. At the same time, the concentration of glycocholic acid in the bile of rats decreased after 20 weeks of rats’ staying on HCD, the concentration of cholic acid did not undergo statistically significant changes. In this case, the conjugation coefficient remains unchanged and the hydroxylation coefficient has decreased which indicates the activation of some alternative pathway of biosynthesis of bile acids. The incidence of nonalcoholic steatosis, also called as a nonalcoholic fatty liver disease, is growing rapidly around the world. The growth rate is 30-40 %, which is becoming a huge medical and economic burden for patients and states in the whole [1]. Therefore, the establishment of mechanisms of pathogenesis of steatohepatosis, its consequences and the development of optimal methods of treatment is an urgent problem of our time. Previously, we have shown that the presence of rats on a high-calorie diet No. 11024 (HCD) (diet No. 11024, Research Diets, New Brunswick, NJ) for 20 weeks leads to the development of metabolic syndrome (MS), on the background of which liver steatosis was recorded [2,3]. On the background of structural changes in the liver, there is a change in its functional state, for the assessment of which it is important to determine the spectrum of bile acids in the bile and to establish a ratio between individual bile acids. Under physiological conditions, this spectrum is quite stable. However, in case of impairments of the hepatobiliary system, the content of individual bile acids can vary significantly. Despite the fact that HCD No. 11024 is widely used in experimental studies, the aim of this work was to investigate the range of bile acids cholates in the bile of rats after 20 weeks of their staying on this diet. Materials and methods. Studies were carried out on male white rats with an initial mass of 210-215 grams. During the first week all rats received the standard food and water ad libitum. On the 2nd week the animals were randomized into two groups. Rats of group 1 received the standard food and water ad libitum and served as a control group. Rats of group II were on the HCD (diet No. 11024, Research Diets, New Brunswick, NJ), which consisted","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74229098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-05DOI: 10.25040/ECPB2019.02.066
O. Kutelmakh
{"title":"Assessment of Dental Status in Rats with Experimental Hyperhomocysteinemia and its Correction with Choline and Vitamin D","authors":"O. Kutelmakh","doi":"10.25040/ECPB2019.02.066","DOIUrl":"https://doi.org/10.25040/ECPB2019.02.066","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79372405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-05DOI: 10.25040/ECPB2019.02.051
O. Dulo
{"title":"The Comparative Characteristics of Aerobic and Anaerobic Productivity of Girls in Lowland and Mountainous Transcarpathian Areas According to the Body Component composition","authors":"O. Dulo","doi":"10.25040/ECPB2019.02.051","DOIUrl":"https://doi.org/10.25040/ECPB2019.02.051","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"688 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91449069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-05DOI: 10.25040/ECPB2019.02.072
I. Terletskyi, M. Verkhola, M. Antoniv, Y. Orel
Chronic wounds constitute a significant challenge confronting patients and health care professionals. In the USA approximately 5.7 million patients are currently suffering from chronic wounds. Expenses on their treatment are estimated at $20 billion annually [1,2]. Lower-extremities ulcers, especially in individuals older than 65 years, are a frequent cause for attending a podiatrist, wound care specialist, primary care physician, vascular surgeon or dermatologist. The bulk of vascular leg ulcers is chronic or recurrent. They can present significant complications among patients with the peripheral vascular disease, including work incapacity. The management of vascular leg ulcers puts a considerable burden on a patient and the health care system. Moreover, these non-healing ulcers increase the risk for lower extremity amputation [3]. Vascular leg ulcers are often multifactorial and can be caused by both arterial and venous diseases. Hypertension and atherosclerosis of peripheral vessels lead to the arterial disease associated with ischemic ulcers. In some cases, difficulties with determining the underlying cause of wound formation complicates treatment and prevents the recurrences. Systemic sclerosis (or scleroderma) is a rare autoimmune connective tissue disorder which is associated with a various degree of systemic manifestations [4]. The organs most frequently affected by scleroderma are skin, gastrointestinal tract, lungs, kidneys, skeletal muscle, and pericardium [5]. One of the challenging complications of systemic sclerosis are non-digital lower extremity ulcers seen both in limited and diffuse scleroderma, with not exactly clear etiology that tends to reflect chronic vasculopathy. They aggravate some pain and ability of the advanced disease. As the incidence of nonhealing lower extremity ulcers in scleroderma has not specifically been studied, more researches are considered to be appropriate in this field of study. The delayed wound healing is typical for systemic sclerosis and, likewise in other chronic leg ulcers, characterized by multifactorial etiology. A role of vascular disease has been proposed, but in spite of restoring the good blood flow and venous drainage, many scleroderma ulcers still remain refractory [6,7]. The 73-year-old female patient V. was sent to the surgical department No 1 of Lviv regional clinical hospital in 2013 due to ulcers on both legs, which she developed 3 years before the clinical attendance. Her medical history was remarkable for myocardial infarction which she suffered in 2009. She suffered from atrial fibrillation (treated by Varfarin 5 mg) and hypertension (treated by Valsartan 80 mg plus Hydrochlorothiazide
{"title":"Successful Treatment of Multiple Chronic Leg Wounds - a Case Report","authors":"I. Terletskyi, M. Verkhola, M. Antoniv, Y. Orel","doi":"10.25040/ECPB2019.02.072","DOIUrl":"https://doi.org/10.25040/ECPB2019.02.072","url":null,"abstract":"Chronic wounds constitute a significant challenge confronting patients and health care professionals. In the USA approximately 5.7 million patients are currently suffering from chronic wounds. Expenses on their treatment are estimated at $20 billion annually [1,2]. Lower-extremities ulcers, especially in individuals older than 65 years, are a frequent cause for attending a podiatrist, wound care specialist, primary care physician, vascular surgeon or dermatologist. The bulk of vascular leg ulcers is chronic or recurrent. They can present significant complications among patients with the peripheral vascular disease, including work incapacity. The management of vascular leg ulcers puts a considerable burden on a patient and the health care system. Moreover, these non-healing ulcers increase the risk for lower extremity amputation [3]. Vascular leg ulcers are often multifactorial and can be caused by both arterial and venous diseases. Hypertension and atherosclerosis of peripheral vessels lead to the arterial disease associated with ischemic ulcers. In some cases, difficulties with determining the underlying cause of wound formation complicates treatment and prevents the recurrences. Systemic sclerosis (or scleroderma) is a rare autoimmune connective tissue disorder which is associated with a various degree of systemic manifestations [4]. The organs most frequently affected by scleroderma are skin, gastrointestinal tract, lungs, kidneys, skeletal muscle, and pericardium [5]. One of the challenging complications of systemic sclerosis are non-digital lower extremity ulcers seen both in limited and diffuse scleroderma, with not exactly clear etiology that tends to reflect chronic vasculopathy. They aggravate some pain and ability of the advanced disease. As the incidence of nonhealing lower extremity ulcers in scleroderma has not specifically been studied, more researches are considered to be appropriate in this field of study. The delayed wound healing is typical for systemic sclerosis and, likewise in other chronic leg ulcers, characterized by multifactorial etiology. A role of vascular disease has been proposed, but in spite of restoring the good blood flow and venous drainage, many scleroderma ulcers still remain refractory [6,7]. The 73-year-old female patient V. was sent to the surgical department No 1 of Lviv regional clinical hospital in 2013 due to ulcers on both legs, which she developed 3 years before the clinical attendance. Her medical history was remarkable for myocardial infarction which she suffered in 2009. She suffered from atrial fibrillation (treated by Varfarin 5 mg) and hypertension (treated by Valsartan 80 mg plus Hydrochlorothiazide","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"132 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73509268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-05DOI: 10.25040/ECPB2019.02.011
O. Mel’nyk, O. Lukyanchenko, O. Gozhenko, I. Popovych
In animal experiments and clinical observations, it was found out that the reactions of the immune system to stress are characterized by features of individuality due to the individual reactions of the main stress-realizing systems: sympatho-adrenomedulary and hypothalamic-pituitary-corticoadrenal [1,2,3,4,5,6]. The secondary, but essential role is also played by stress-induced changes in vagus tone, blood levels of sex and thyroid hormones as well as parathyrin and calcitonin [6,7,8,9,10,11,12]. In order to find out the role induced by chronic stress changes in immune parameters of the sympatho-vagal balance in the previous study, we [13,14] compared the individuals who were retrospectively divided into two groups, almost identical to the average value of LF/HF ratio and its dispersion, but with opposite deviations from the standards of the links of immunity. In stress-sensitive individuals the integral state of Phagocytosis as Z-scores was compiled: –1,82 ± 0,39, of Cellular link: –1,69 ± 0,21, of Humoral link: +0,23 ± 0,19 versus +0,76 ± 0,43; +0,23 ± 0,26 and +0,76 ± 0,11 in stress-resistant persons respectively. The immune profiles constructed on Z-scores can be divided into three networks. The first set contains 7 parameters (IL-6, Entropy of Leukocytogram (LCG), Killing Index vs E. coli and Staph. aureus, Microbial Count E. coli, Igg G and M) that are not significantly different from persons who are stress-sensitive or stress-resistant. 18 parameters of the second set (total Leukocytes, Segmented and Stub Neutrophils, Phagocytose Index vs E. coli and Staph. aur., Microbial Count Staph. aur., Bactericidity vs E. coli and Staph. aur., T-active, T-helper, T-cytolytic, Natural Killers and B-Lymphocytes, IgA, CIC, Popovych’s Adaptation Index of LCG as well as Bifidobacterium and Lactobacillus feces) to a greater or lesser degree are higher in stress-resistant persons. The 8 parameters of the third set (total and 0-Lymphocytes, Monocytes and Eosinophils, Popovych’s Strain Index of LCG, TNF-α, IL-1 as well as Hemolytica E. coli) are higher in stress-sensitive persons. Previously, in our laboratory, significant relationships were found between the parameters of phagocytic, cellular and humoral links of immunity, on the one
在动物实验和临床观察中发现，由于主要的应激实现系统交感神经-肾上腺髓质和下丘脑-垂体-肾上腺皮质的个体反应，免疫系统对应激的反应具有个体化特征[1,2,3,4,5,6]。应激引起的迷走神经张力、血液中性激素、甲状腺激素以及甲状旁腺素和降钙素水平的变化也起着次要但重要的作用[6,7,8,9,10,11,12]。为了研究慢性应激变化对交感迷走神经平衡免疫参数的影响，我们[13,14]将回顾性分为两组的个体进行比较，两组的LF/HF比值平均值及其离散度几乎相同，但与免疫环节的标准偏差相反。在应激敏感个体中，吞噬作用的整体状态作为z分数被编译为:- 1,82±0,39，细胞连接:- 1,69±0,21，体液连接:+0,23±0,19 vs +0,76±0,43;应激抵抗者分别为+0,23±0,26和+0,76±0,11。基于z分数构建的免疫图谱可分为三个网络。第一组包含7个参数(IL-6、白细胞熵(LCG)、对大肠杆菌和葡萄球菌的杀伤指数)。金黄色葡萄球菌，微生物计数大肠杆菌，Igg G和M)，与应激敏感或应激抗性的人没有显著差异。第二组18个参数(白细胞总数，分节和短中性粒细胞，吞噬指数vs .大肠杆菌和葡萄球菌。本身。微生物计数;葡萄球菌;本身。细菌性vs大肠杆菌和葡萄球菌。本身。抗逆性人群中t活性、t辅助、t细胞溶解、自然杀伤和b淋巴细胞、IgA、CIC、LCG以及双歧杆菌和乳杆菌粪便的Popovych适应指数或多或少都较高。应激敏感者的第三组8个参数(总淋巴细胞和0淋巴细胞、单核细胞和嗜酸性粒细胞、LCG、TNF-α、IL-1和溶血性大肠杆菌的波波维奇菌株指数)较高。以前，在我们的实验室中，我们发现免疫的吞噬、细胞和体液环节的参数之间存在显著的关系
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