Experimental and Clinical Physiology and Biochemistry最新文献

{"title":"Characteristic of changes in the blood transaminases activity in the dynamic of the combined pathology – immobilization stress and adrenaline-induced myocardial injury and their correction of L-arginine","authors":"","doi":"10.25040/ecpb2020.03-04.026","DOIUrl":"https://doi.org/10.25040/ecpb2020.03-04.026","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74977383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological features of leptin in patients with diabetes type 2 and eating disorders","authors":"","doi":"10.25040/ecpb2020.03-04.020","DOIUrl":"https://doi.org/10.25040/ecpb2020.03-04.020","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78294515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ammonium glycyrrhizinate has a protective effect on oogenesis and reduces genotoxic stress and death of immune cell in lipopolysaccharide-induced endotoxemia","authors":"O. Kondratska","doi":"10.25040/ECPB2020.03-04.005","DOIUrl":"https://doi.org/10.25040/ECPB2020.03-04.005","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82244246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphisms of paraoxonase genes – the common background of gallbladder and heart diseases","authors":"","doi":"10.25040/ecpb2020.02.064","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.064","url":null,"abstract":"","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84175613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrostimulation with the devices «VEB-1» and «VEB-2» causes almost identical changes in the parameters of gas-discharge visualization","authors":"N. Babelyuk","doi":"10.25040/ecpb2020.02.005","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.005","url":null,"abstract":"Introduction . In 2015, a generator for electrotherapy and stimulation oh human nerve centers was created, called “VEB-1”. Preliminary observation of volunteers revealed a modulating effect of a four-day course of electrical stimulation on the parameters of electroencephalogram, metabolism, as well as gas-discharge visualization (GDV). Recently designed device \"VEB-2\". This report launches a series of articles on a comparative study of the course effects of the devices \"VEB-1\" and \"VEB-2\" on the human body. GDV was chosen as the first method. Material and research methods. The object of observation were 18 volunteers: 11 women aged 29-62 years (Mean±SD: 51±12) without clinical diagnose but with dysfunction of neuro-endocrine-immune complex and metabolism. In the morning registered kirlianogram by the method of GDV by the device of “GDV Chamber”. After the initial testing, an electrical stimulation session was performed with a VEB-1 or a VEB-2 devices/ The next morning after completing the four-day course, retesting was performed. The reference values of the parameters are taken from the database of our laboratory. the following [17], then the price change of the latter parameters will wait. The effects on the set of GDV parameters are almost equally pronounced in people of both sexes when using both devices. Conclusion. The use of an additional electrode for direct impact on individual organs in the device \"VEB-2\" is excessive, it is enough to affect only the nerve plexuses.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82347713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid Peroxidation Processes in Loach Embryos under the Effect of Microwave Radiation","authors":"A. Tarnovska, Yu. Shcorbatov","doi":"10.25040/ecpb2020.02.030","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.030","url":null,"abstract":"Various physical and chemical factors influence leads to disruption of pro-oxidant and antioxidant systems. Interaction between electromagnetic field and living cell and organism are complicated and is currently obscure. Well known that embryonic objects are sensitive to the effects of radiofrequency electromagnetic radiation (RF EMR). The aim of this study was to evaluate the effect of microwave radiation on free radical processes changes in loach embryos. The experiment has been carried out on loach embryos ( Misgurnus fossilis L.). Zygotes were exposed to microwave radiation created by electromagnetic wave (EMW) generator and immedi-ately after fertilization (5, 10 and 20 minutes exposure). EMW generator with 0,00197 mW/cm 2 energy intensity was in a distance of 3 centimeters above the Petri dishes. In selected samples determined the intensity of lipid peroxidation process by the content of secondary product of lipid peroxidation by Timirbulatov method. Quantitation of total protein content in the membrane preparation was determined by Lowry method. Statistical processing of experimental data was carried out by two-way analysis of variance. The results of recent studies indicate that microwave radiation leads to significant activation of free radical processes. Current data indicates that effect of radiofrequency EMR at 5, 10 to 20 minutes duration causes reduction of lipid peroxidation at early stages of embryos development. It was established that in the early hours of germs microwave radiation at 5 minutes leads to significant increasing of MDA level by 16,5±3,5 %, but on stage 16 blastomers there were tendency to the elevation as compared to control (P > 0.05). However, on stage 64, 256 and 1024 blastomers was found significant decreasing of MDA level by 21–31 % under the effect of RF EMR at 5 minutes duration. Same tendency in MDA level changes observed under the action of 10 min exposure of low-intensity microwave radiation. Higher exposure of radiofrequency EMR (20 minutes) leads to a significant decrease in MDA levels by 26–42 % on stage 2, 16, 64 and 256 blastomers compared with the control. However, 20 minutes exposure leads to not confidentially significant MDA level changes at the last stage of synchronous divisions of blastomers (X division, 330 minutes). Analysis of variance results specify the significant EMR influence at 20 minute on lipid peroxidation indexes (59,6 %). The results suggested that the influence of low-intensity microwave radiation (1850 MHz) leads to disruption of lipoperoxidation processes. Further studies on the effects of radiofrequency EMR on the enzyme activity of antioxidant defense system of loach embryos are needed.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88941509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the effect of body fat content, measured by different methods, on the indicators of central hemodynamics","authors":"","doi":"10.25040/ecpb2020.02.057","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.057","url":null,"abstract":"OBJECTIVES Investigate whether the effect of body fat on central hemodynamics is different in the body mass index (BMI) and fat distribution model using a bioimpedance method. BACKGROUND Central hemodynamic indicators are independent predictors of the risk of cardiovascular disease (CVD) development and progression. Obesity is a well-known risk factor for metabolic disorders and one of the first triggers of the mechanism of atherosclerosis. Excess weight leads to maladaptive modification of myocardium. However, another scientific works point on positive effect of overweight on prognosis and risk of development of CVD, so called “paradox of obesity”. For example, patients with hypertension and coronary heart disease (CHD) who were overweight had a lower mortality rate than the normal weight group. Another study also reported overall mortality from both CVD and noncardiovascular disease, which was higher in the group with the lowest BMI. Whereas another large study indicated a significantly increased risk of coronary heart disease and stroke in groups with a BMI> 25 compared with the normal weight group. In our study, we wanted to investigate whether the influence of body fat distribution, measured by various methods, on hemodynamic parameters differs. METHODS The survey was attended by 174 women who had their body composition indicators measured using a TANITA BC-601 weight analyzer and a BMI calculation. A total of 151 healthy women, aged 26–53 years, were selected for the survey (exclusion criteria were acute and chronic diseases, menopause, lactation or pregnancy) who were investigated for the parameters of central hemodynamics using the ReoCom (KhAI-Medica) rheographic complex. We made two models for statistical analysis. In the first model we divided women on groups depending on their BMI. In the second model we used bioimpedance method for grouping (depending on total fat content in percent, according to their age). Statistical analysis were processed using ANOVA analysis of variance and using the hypothesis of equality of the two means using Stu-dent’s t-test in the program STATISTICA 10.0 (StatSoftInc, USA). RESULTS Dispersion analysis showed that in the BMI fat grouping model, fat had an effect on more parameters of central hemodynamics than in the total fat% grouping model In the first model fat had statistically significantly effect on the following parameters: stroke volume, total blood volume, total peripheral vascular resistance, left ventricle work, index of left ventricle work, left ventricle capacity . In the BMI model, most indicators differed statistically significantly in all three groups (obesity, overweight and normal fat content), whereas in the other model, the hemodynamic indicators had a significant difference between the obese and normal fat groups. However, the mean of parameters of central hemodynamics in the respective groups were not significantly different in the two models. CONCLUSIONS The data obtained method for ","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87282190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pubertal development of male offspring born to mothers of different ages with fetoplacental insufficiency","authors":"","doi":"10.25040/ecpb2020.02.036","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.036","url":null,"abstract":"Today, the problem of male infertility or poor reproductive health is very relevant around the world. The formation of the reproductive system begins in the womb, when all the organs and systems are formed. Fetoplacental insufficiency – a clinical syndrome caused by morpho logical and functional changes in the placenta, manifested by disorders of the fetus, growth and development. To date, it is not known how fetoplacental insufficiency in mothers affects the condition and functioning of the reproductive system in adult male offspring. The purpose of this scientific work was to investigate the development of puberty in males-offspring born to mothers of different age with fetoplacental insufficiency and to evaluate efficacy of base and combined drug therapy during pregnancy. Materials. Healthy, mature Vistar females-mothers of young (3–4 months) and mature (8–10 months) reproductive age have been used in the experiment. Modeling of fetoplacental insufficiency has been carried out by subcutaneous daily introduction of 50% tetrachlomethane oil solution in dose of 2ml/kg of body weight from 12 th to 18 th day of pregnancy. General body masses and sexual development have been researched. The samples of blood serum with the aim to evaluate estradiol, testosterone and progesterone hormones levels have been taken. Results. Discussion. In the study of the reproductive weight of males, no changes were found, but at the autopsy of not lowered into the scrotum (cryptorchidism), such rats were born to young mothers with feto placental insufficiency. Such changes occurred against the background of hormonal imbalance, which manifested itself in a decrease in total testosterone and, conversely, increased estradiol and progesterone. There was a lag in sexual development in animals born to mothers of two age groups with fetoplacental insufficiency. Pharmaceutical composition contributed to normal physiological sexual development in males born to mothers of mature reproductive age with fetoplacental insufficiency, in young females, pharmaceutical composition did not show any corrective effect on offspring as well as the comparison drug – Dipyridamole. Conclusions and prospects of further developments. Fetoplacental insufficiency affects the somato-sexual development of male offspring born to mothers of different reproductive ages, which is manifested in delayed puberty and imbalance of sex hormones (decreased testosterone in all animals and increased estrogen levels only in offspring born to reproductively young). Which in the future can lead to problems with the functioning of the reproductive system. In the application of the new pharmaceutical composition which is administered to correct abnormal condition of pregnancy and observed high levels of testosterone but not estradiol levels decrease high only in offspring born to young females, which subsequently led to the normal sexual development of males. The comparison drug Dipyridamole did not show a corrective ","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72835492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Searching efficient antibacterial agents and active metabolites of streptomycetes with relatively high-resistance to clinical isolates of bacteria","authors":"","doi":"10.25040/ecpb2020.02.052","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.052","url":null,"abstract":"The aim of our work was to study the species profiles of microorganisms that complicate the course of trophic ulcers of the lower extremities, determination of the sensitivity spectrum of isolated strains to antibacterial drugs and the search for effective metabolites of streptomycetes relative to clinical isolates of microorganisms. During the research were examined 65 patients with chronic trophic ulcers and clinical material was collected by using sterile cotton swabs. A total of 113 strains of microorganisms were isolated. P. aeruginosa was the most common pathogen in 45 patients, amounted to in monoculture 12 cases (18.46%±4.81%). The second place of the terms for frequency were isolation among of contaminants of trophic wounds is S.aureus , isolated in 33 patients, which is 50,77%±6,2%, and in monoculture isolated – only 4 strains (6,15%±2,98%). The third place is occupied by S. epidermidis , isolated from 10 patients (15,38%±4,47%), in monoculture it was detected in one case (1,54%±1,53%). Klebsiella pneumoniae , which is 4,62%±2,6%, and Proteus vulgaris (1,54%±1,53%) were detected in 3 cases in the monoculture. Other microorganisms are isolated only in associations. The most common association of S.aureus and P.aeruginosa Streptococcus spp. P.aeruginosa (3,08%); Acinetobacter spp. S.aureus (3,08%); Klebsiella spp. and S. epidermidis (1,54%); E.coli Proteus spp . (1,54%); P.aeruginosa Acinetobacter spp. (1,54%); Acinetobacter spp. and S.epidermidis (1,54%); Cit robacter spp. and S. aureus (3,08%); Peptostreptococcus spp. and P.aeruginosa (3,08%); S.epider midis and Proteus spp . Klebsiella spp. and S. aureus Peptostreptococcus spp. and S. aureus (1,54%); Enterobacter spp., Candida albicans and S.aureus Enterococcus spp., Klebsiella pneumoniae and S. epidermidis actinomycetes. According to this technique, screening of 369 actin omycetes isolates isolated from the juniper sphere of high Juniperus excelsa Bieb was performed. Among the streptomycetes isolates tested, seven of them were detected with activity against the reference strain P. aeruginosa ATCC 9027. The active metabolites of these streptomycetes were tested for activity against P. aeruginosa clinical isolates. One strain of actinomycetes Je 1–79 was found to exhibit high inhibitory activity against all clinical isolates of pseudomonads. Active metabolites of Je 1–79 are promising to create a tool for topical use in patients with P. aeruginosa infected trophic ulcers.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78324849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of whole body vibration on structural properties of bone in conditions of obesity and limited mobility","authors":"","doi":"10.25040/ecpb2020.02.014","DOIUrl":"https://doi.org/10.25040/ecpb2020.02.014","url":null,"abstract":"In this study we assessed the impact of high-frequency whole body vibration with acceleration of 0,3 g on the structure of the femur in in conditions of obesity and limited mobility. It is known that mechanical loads stimulate bone remodeling by reducing the apoptosis of osteoblasts and osteocytes, increasing their proliferation and differentiation. Reducing the functional load inhibits osteogenesis and activates bone resorption. mineral composites at the end of the experiment. Therefore, ex-ercises are an effective method to improve bone condition and should be used as an adjunct to pharmacological therapy for osteoporosis.","PeriodicalId":12101,"journal":{"name":"Experimental and Clinical Physiology and Biochemistry","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77869652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}